| bee-sting anaphylaxis in childhood. | the syndrome of bee-sting anaphylaxis is described. children who have suffered crescendo reactions to previous bee stings, especially children with a history of asthma, are significantly at risk. desensitization is required in such cases; adrenaline should be kept in the home, and parents should be trained in its emergency use. the immunological mechanisms of bee-sting anaphylaxis are described. the striking seasonal incidence of anaphylaxis suggests that pollen or plant products which are incor ... | 1979 | 514147 |
| flower-feeding specialization in wild bee and wasp communities in seasonal neotropical habitats. | flower visitation records were obtained for 192 species of bees and 138 species of wasps in three neotropical habitats. four indices of feeding specialization were calculated for common species, and similar patterns were observed using each index. variation in specialization could be attributed to several factors: the habitat and season in which species were found, the degree of sociality of species, and whether species were bees or wasps. important differences among habitats and seasons were th ... | 1979 | 28309659 |
| [comparative analysis of thermoregulation in the nests of certain species of social insects]. | studies have been made on thermal regulation in the nests of families of the honey bee apis mellifera, wasp dolihovespula silvestris and bumblebees bombus terrestris, b. agrorum and b. lapidaris during their maximum development. it was shown that thermoregulation significantly stimulated the brood in the nest. among the species investigated, the highest thermoregulatory capacities are exhibited by honey bees, this fact being associated with the large number of individuals in their colonies. duri ... | 2010 | 506590 |
| ige antibodies to bee venom, phospholipase a, melittin and wasp venom. | specific ige antibodies against bee venom, phospholipase a, melittin and wasp venom have been examined in fifty patients with an unusually severe reaction after bee or wasp sting. two thirds of the bee venom-sensitive patients also have detectable ige antibodies to wasp venom. more than 50% of the wasp venom-sensitive patients are also allergic to bee venom. phospholipase a and melittin ige antibodies were found, respectively, in two thirds and one third of the bee venom-sensitive cases. specifi ... | 1979 | 498494 |
| the first optic ganglion of the bee. iii. regional comparison of the morphology of photoreceptor-cell axons. | the nine receptor cells in each ommatidium of the worker bee end as six short visual fibres in the lamina and as three long visual fibres in the medulla. behavioural and physiological evidence for regional variation in spectral sensitivity prompted observations on the morphology of the visual units. the distribution, branching pattern, diameter and the arrangement of axonal protusions of the characteristic receptor-cell axons were studied in various regions of the lamina. the six short visual fi ... | 1979 | 487403 |
| measurement of the potency of pollen extract by radioimmunoprecipitation. | we describe a novel procedure for standardization of allergen extracts using radioiodinated crude extracts. radiolabeled extracts were reacted with immunoglogulin e (ige) antibody, and the resulting complexes precipitated by anti-ige. experiments with ragweed pollen, grass pollen, honeybee venom, and alternaria extracts revealed that 1% to 14% of the radioactivity was precipitated, these percentages ranged from 2 to 20 times blank values. binding of labeled ragweed extracts to ige antibody could ... | 1979 | 469117 |
| [the basophil degranulation test in the diagnosis of bee and wasp sting allergies]. | comparison of basophil degranulation test (bdt) with radioallergosorbent test (rast) in 50 patients with anamnestically certain or probable hymenoptera allergy yielded practically equal results. in patients who had experienced their allergic reaction a long time ago, bdt seemed to react positively over a longer period of time than rast. as bdt is technically easy to perform and moderate in price, it is suited to substitute rast. | 1979 | 493908 |
| [varroatosis of the honey bee]. | | 1979 | 383454 |
| comparison of skin tests and rast for the diagnosis of bee sting allergy. | skin tests and estimation of specific ige-antibodies by the rast are evaluated as diagnostic procedures in bee sting hypersensitivity with the followiny extracts. (2) with bee venom results of skin tests and rast correspond in 82%, with bee wholebody extracts in 68%. (3) both with skin tests and the rast with bee venom a good discrimination between patients with bee sting hypersensitivity and non-allergic controls is observed. skin tests with bee venom are somewhat more sensitive than bee venom- ... | 1979 | 517710 |
| enzymes of bee venom, sac and whole body. | by using the api-zym system 55 enzymes were determined to be present in bee venom, venom sac, sacless whole body extract and whole body commercial extracts. acid phosphatase activity was high in bee venom and sacless whole body extracts. arylamidase activities were elevated in sacless whole body extracts and very low or undetectable in venoms. conversely, glycyl-prolin arylamidase activity was high in venoms and very low in the other extracts. | 1979 | 37787 |
| influence of enzymatic phospholipid cleavage on the permeability of the erythrocyte membrane. ii. protein-mediated transfer of monosaccharides and anions. | in order to investigate the influence of membrane lipids on transport via the protein domain of the erythrocyte membrane, a number of facilitated diffusion processes was studied by tracer flux techniques in whole cells after cleavage of up to 65% of the phosphatidylcholine or the sphingomyelin by phospholipase a2 from naja naja or bee venom, or by sphingomyelinase, respectively. the mediated fluxes of l-arabinose, which is transported by the glucose carrier, and of l-lactate, which uses a specif ... | 1979 | 486450 |
| natural history of allergy to hymenoptera. | one hundred and nineteen patients who had a generalized reaction to a bee sting and who subsequently had a history of being re-stung before desensitization were studied. the reaction, in these 119 patients was as follows: 44.5% had an improved grade of reaction, 42.9% had the same grade of reaction and 12.6% had a worse grade of reaction. of these 119 patients, 39.5% had only a local reaction to their re-sting. those patients who were re-stung within 2 weeks (anergic period) or over 5 years afte ... | 1979 | 476909 |
| [pathology of the honeybee]. | | 1979 | 462784 |
| isolation of the cytoplasmic form of malate dehydrogenase from honey bee (apis mellifera) larvae. | | 1979 | 111673 |
| insects as inhalant allergens. | the importance of insect dust as inhalant allergen (e.g. locust, cockroach, mite, butterfly, bee, fly, etc.) is frequently underestimated as a cause of allergic conjunctivitis, rhinitis and bronchial asthma. the high allergenic potency of insect dust is demonstrated in cases of occupational allergy against locust. this dust is an obligatory sensitizing agent depending upon the degree of exposure and the potency of the allergen irrespective of constitutional factors. no doubts exist any more abou ... | 2006 | 474330 |
| rush venom immunotherapy program for honeybee sting sensitivity. | | 1979 | 429714 |
| [death from a bee sting]. | | 1979 | 442781 |
| natural polyesters: dufour's gland macrocyclic lactones form brood cell laminesters in colletes bees. | bees in the genus colletes make their brood cells in the ground and coat them with a highly resistant, waterproof, transparent membrane. this membrane is a polyester constructed mainly from 18-hydroxyoctadecanoic acid and 20-hydroxy-eicosanoic acid, which are stored as their corresponding lactones in the dufour's gland of the bee. when lining the cells, the bee secretes its glandular content, and the membrane is apparently a product of polycondensation reaction of its contents. this appears to b ... | 1979 | 17758016 |
| enzyme-linked immunosorbent assay of allergen-specific igg antibodies in bee sting allergic patients hyposensitized with pure bee venom. | an elisa is presented for detection of igg antibodies to bee venom. by this method, sera of 11 bee sting allergic patients, who were treated with rapid hyposensitization with pure bee venom, were tested. the highest antibody titers were observed after 30 days of treatment, a maximum rise of 7.4 +/- 1.5 log 2-titer steps. pure bee venom is shown to be more potent immunologically than whole body bee extract. prediction of the clinical success, measured by tolerance to a bee sting challenge, is not ... | 1979 | 459376 |
| the use and interpretation of rast to stinging insect venoms. | quality controlled, standardized rast systems for five stinging insect venoms are described. these systems were evaluated using 375 sera from insect reactive patients from six diverse geographic regions. ninety-four percent of patients with recent histories of systemic allergic reactions to stings were positive to at least one venom while 4 percent of normal local reactors were positive. the cross reactions between honey bee and vespid venoms were studied by rast inhibition. a quantitative metho ... | 1979 | 434584 |
| population dynamics of sex-determining alleles in honey bees and self-incompatibility alleles in plants. | mathematical theories of the population dynamics of sex-determining alleles in honey bees are developed. it is shown that in an infinitely large population the equilibrium frequency of a sex allele is 1/n, where n is the number of alleles in the population, and the asymptotic rate of approach to this equilibrium is 2/(3n) per generation. formulae for the distribution of allele frequencies and the effective and actual numbers of alleles that can be maintained in a finite population are derived by ... | 1979 | 17248901 |
| [epidemiological studies on the symptoms due to strawberry culture in the vinyl-house. 2. an analysis of clinical examinations with emphasis on allergic response (author's transl)]. | clinical mass surveys were carried out on the residents to whom questionnaires on symptoms with reference to strawberry culture in the vinyl-house had been delivered. two hundred and eleven persons attended at the first survey in september, 1976 (off the season of strawberry culture) and the 58 attended at the second survey in march, 1977 (within the season). the participants of the first survey consisted of three groups, i.e. farmers with and without strawberry culture and people without farmin ... | 1979 | 491286 |
| solid phase synthesis of 13-lysine-apamin, 14-lysine-apamin and the corresponding guanidinated derivatives. | in order to study the importance of arginine residues 13 and 14 in apamin, the bee venom neurotoxin, four analogues, [lys13]-apamin, [lys14]-apamin, [har4, har13]-apamin and [har4, har14]-apamin were synthesized and tested with respect to their neurotoxicity. the two lysine-apamins were prepared by the solid phase method on benzhydrylamine resins. before oxidation to disulphides, the (s-acm)4-peptides were isolated and characterized. portions of the purified lysin peptides were converted to homo ... | 1979 | 429106 |
| desensitization of patients with bee sting allergy using pure bee venom. | forty patients who had previously experienced severe systemic reactions after a bee sting were desensitized using pure bee venom. a modified 'rush' regimen was employed whereby patients received two injections a week and reached maximal desensitization in 5 weeks. eleven patients have subsequently been stung again and have developed no generalized reaction. although this form of desensitization is considered to be highly effective in protecting sensitive patients, both generalized and local side ... | 1979 | 375434 |
| [diagnosis of insect-toxin allergy (bee, wasp) using the radioallergosorbent test]. | | 1979 | 425600 |
| the localization of honey bee thorax trehalase. | differential and sucrose gradient centrifugation of honey bee thoraces, disrupted by gentle methods and using mannitol-triethanolamine-edta buffer at ph 6.5, showed that in the honey bee thorax 92-94.8% of the trehalase was mitochondrial. since only 92-95% of the cytochrome c oxidase, a known mitochondrial enzyme, was found in the mitochondrial fraction by these methods, it was concluded that honey bee trehalase is totally mitochondrial. significant amounts of 'microsomal' or 'soluble' trehalase ... | 1979 | 455114 |
| anticholinesterase activity and knock-down of substituted phenyl n-methylcarbamates to honey bee. | | 1979 | 444724 |
| x-ray microanalytical studies on native myofibrils and mitochondria isolated by microdissection from honey-bee flight muscle. | the distribution of elements was studied by means of energy dispersive x ray microanalysis in the honey-bee flight muscle. semi-quantitative analysis of bulk muscle specimen, isolated mitochondria and isolated myofibrils either in resting or stretched state was performed. high k peak was observed in the spectra of bulk muscle specimen resembling the spectra of myofibrils and high p peak resembling the spectra of mitochondria. in the myofibrils no substantial difference was found between the spec ... | 1979 | 553443 |
| contraction of muscle fibril under the effect of salt solutions without atp. | it is possible to elicite contraction on isolated muscle fibrils of honey-bee in isotonic sugar-solution by adding certain salt solutions without the addition of atp. from this phenomenon we can conclude that the muscle contraction can appear under the effect of electricity. | 1979 | 547669 |
| effects of phospholipase a2 from cobra and bee venom on the presynaptic membrane. | | 1979 | 516081 |
| evaluation of bee sting allergy by skin tests and serum antibody assays. | we studied 55 subjects who had had anaphylactic reactions to bee stings within the previous 3 years. 38 out of 54 tested had ige antibody to honey bee venom (hbv) as measured by radioallergosorbent test (rast). on skin testing, 30 out of 34 had a positive test to hbv. of these, 26 had a positive rast. a positive skin test to hbv at high dilution or else a high anti-hbv rast score appeared to identify those who, in a 6-month follow-up period, were at risk of developing further anaphylaxis followi ... | 1979 | 468389 |
| [zinc content in bee honey and mead from the olsztyn province]. | | 1979 | 451425 |
| relationship between age and hyaluronidase activity in the venom of queen and worker honey bees (apis mellifera l.). | | 1979 | 419541 |
| purification and biochemical characterization of an 11 000-dalton beta-bungarotoxin. | the chromatographic separation and biochemical characterization of a beta-bungarotoxin is described. this toxin is isolated as the most basic eluting protein of bungarus multicinctus venom when separated by column chromatography on cm-sephadex c-25. the protein migrated as a single band on ph 4.3 and sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis. the molecular weight of this toxin was estimated to be 10 000 +/- 1000 by analytical sedimentation analysis. this value was consisten ... | 1978 | 569500 |
| [clinical picture and diagnosis of bee-venom allergy. a comparison between skin tests and rast determinations]. | of a group of 53 anamnestically assured bee venom allergies 81.1% positive skin tests (bee whole body extract) as well as 79% positive rast (bee venom) could be proven. a statistical connection between skin test and clinic reaction degree, as well as between "rast-clinic reaction degree" and "skin test--rast" could not be found. the agreement between skin test and rast results was of 71.7%, the positive correlation of 66%. the problem of the diagnosis and the therapy of allergy to hymenopteren s ... | 1978 | 721471 |
| activation of bee venom phospholipase a2 by oleoyl imidazolide. | imidazolide derivatives of long-chain fatty acids are shown to be potent irreversible activators of bee venom phospholipase a2. activation corresponds to the addition of a single acyl residue to the protein molecule. the rate of activation increases with the acyl chain length of the activator, but the highest activation factors are given by the oleoyl and linoleoyl residues. acyl group activation of the enzyme substitutes completely for activation by free fatty acids (but produces 3--4-fold high ... | 1978 | 720331 |
| a bee counter for monitoring bee activity and bee behaviour [proceedings]. | | 1978 | 719316 |
| honey bee venom allergy immunological studies of systemic and large local reactions. | the ige and igg antibody responses to four allergen fractions of honey bee venom were studied in 13 sera from patients who experienced systemic reactions from bee stings and eight from those who experienced large locals. both groups were similar in allergen response. ige and igg levels and ratios. a significant number of patients were found to have experienced both types of reactions from bee stings. it is proposed that ige antibody positive systemic and large local reactions are both ige mediat ... | 1978 | 717848 |
| [hyposensitisation treatment with pure bee venom (author's transl)]. | hyposensitisation with pure bee venom was undertaken in eleven persons with proven allergy to bee stings. rapid hyposensitisation led to side effects in seven of them during dosage increase. serial determination of total iga, igg, igm and alpha1-antitrypsin revealed no changes, while allergen-specific igg and ige and total ige increased maximally after 30 days. all persons were exposed to one bee sting during the treatment: all tolerated it well without the allergic reactions which had previousl ... | 1978 | 308876 |
| competitive interactions between neotropical pollinators and africanized honey bees. | the africanized honey bee, a hybrid of european and african honey bees, is thought to displace native pollinators. after experimental introduction of africanized honey bee hives near flowers, stingless bees became less abundant or harvested-less resource as visitation by africanized honey bees increased. shifts in resource use caused by colonizing africanized honey bees may lead to population decline of neotropical pollinators. | 1978 | 17743636 |
| the use of the radioallergosorbent test in the diagnosis of hymenoptera anaphylaxis. | ige antibody levels to hymenoptera (honey bee, hornet, wasp or yellow jacket) venom or venom sac were measured in the serum of ninety-six patients with a history of immediate hypersensitivity reactions to these insect stings. normal levels of ige antibody were found in fourty-four of these patients tested. the severity of the systemic reaction was similar in those patients with normal and elevated ige antibody levels, and the interval from the systemic reaction to antibody determination was also ... | 1978 | 709792 |
| [oribatid mites in honeybee nests]. | | 1978 | 695308 |
| [effect of melitten from bee venom on mitochondrial functions]. | effect of different concentrations of bee venom melittin on respiration, mitochondrial oxidative phosphorylation, and permeability of mitochondrial inner membranes for different ions is studied. low melittin concentrations (1-2 mcm) increased in several times the rate of h+, k+, na+ ions and tris transport, in a lesser degree--bivalent ions and cl- transport and did not affect the permeability for succrose. thus, the uncoupling effect of low melittin concentrations on mitochondrial energetic app ... | 1978 | 737229 |
| use of rast technique in wasp sting hypersensitivity. cross-reactions between various insect antigens are specially considered. | clinical hypersensitivity to wasp stings was found to be fairly well correlated with the presence of serum ige-antibodies against yellow jacket venom as detected by the rast technique. such antibodies were never found in a control group of non-allergic blood donors, but they were detected in a surprisingly large proportion of patients with bee sting allergy without known allergic reactions to wasps. studies using rast inhibition technique failed to prove cross-reactions between bee and wasp veno ... | 1978 | 717704 |
| the honey bee "language" controversy. | | 1978 | 672244 |
| the interaction of bee melittin with lipid bilayer membranes. | the influence of melittin and the related 8-26 peptide on the stability and electrical properties of bilayer lipid membranes is reported. melittin, unlike the 8-26 peptide, has a dramatic influence on lipid membranes, causing rupture at dilute concentrations. the circular dichroism of melittin demonstrated that under physiological conditions, in water, melittin is in extended conformation, which is enhanced in aqueous ethanol. however in 'membrane-like' conditions it is essentially alpha-helical ... | 1978 | 667038 |
| morphological evidence for calcium stores in photoreceptors of the honeybee drone retina. | | 1978 | 682224 |
| effect of the state of phosphatidylcholine on the rate of its hydrolysis by phospholipase a2 (bee venom). | | 1978 | 677904 |
| comparison of binding of ige and igg antibodies to honeybee venom phospholipase-a. | | 1978 | 659884 |
| hymenoptera sting hypersensitivy: ige, igg and haemagglutinating antibodies to bee venom constituents in relation to exposure and clinical reaction to bee stings. | high levels of ige antibodies (ab) to bee venom constituents were mainly found in patients with bee sting hypersensitivity, whereas the sera of bee keepers usually contained high levels of igg ab and haemagglutinins, representing blocking antibody activity. in bee keepers there was a positive correlation between the degree of severity of an adverse reaction to bee stings and ige ab, a negative one between the severity of reaction and igg ab and haemagglutinins, a positive one between the number ... | 1978 | 668100 |
| relationship of atopy and anaphylactic sensitization: a bee sting allergy model. | | 1978 | 668099 |
| diagnosis of honey bee venom allergy. | studies using both an optimized rast assay and intradermal skin testing with whole bee venom indicate that both methods should be used for diagnosis. rast and skin test are of similar sensitivity but each misses some patients found positive by the other. correlation between the tests was 91% with an additional 5% only rast positive and 4% only skin test positive. | 1978 | 646187 |
| [the role of the glial cells in the maintenance of the ionic environment of the photoreceptors of the retina of the drone (author's transl)]. | a double-barrelled potassium sensitive microelectrode was used to record electrical potentials and k+ activities in the retina of the drone apis mellifera during stimulation with trains of flashes, 1 per sec, intense enough to produce receptor potentials of near maximal amplitude. during the stimulation photoreceptors lose about 25% of their intracellular potassium concentration. during stimulation the potassium activity in the extracellular space increased transitorily up to 20 mm and then fell ... | 1978 | 651211 |
| solid phase synthesis of apamin, the principal neurotoxin in bee venom. isolation and characterization of acetamidomethyl apamin. | the synthesis of apamin, the principal neurotoxin in bee venom, has been accomplished by the solid phase method on a benzhydrylamine resin, 2-phenylisopropyloxycarbonyl amino acids were used throughout the synthesis except for the c-terminal histidine. improved yields in the coupling steps in the n-terminal part of the molecule were obtained by coupling each amino acid both in dichloromethane and dimethylformamide. the use of acetamidomethyl as an s-protecting group for cysteine made it possible ... | 1978 | 649258 |
| allergens in hymenoptera venom. v. identification of some of the enzymes and demonstration of multiple allergens in yellow jacket venom. | yellow jacket venom (yjv) contains acid phosphatase, hyaluronidase and phospholipase but neither allergen c nor melittin as bee venom (bv). yjv enzymes did not cross react with antisera to bv enzymes. yjv was separated into seven fractions, all of which had some activity in rast. sera were found specific for the fractions containing phospholipase and acid phosphatase. the other three protein fractions also exhibited substantial rast activity, suggesting there are at least five allergens in yjv. | 1978 | 637376 |
| the structures of some peptides from bee venom. | the sequences are given of two peptides (secapin and melittin f) whose isolation from the venom of the common european honey bee (apis mellifera) has previously been described [gauldie et al. (1976) eur. j. biochem. 61, 369--376]. some structural studies on the known peptide 401 (mcd peptide) are described. the positions of the two disulphide bridges in peptide 401 have been determined by (an essentially) novel method. | 1978 | 631126 |
| the preparation of activated bee venom phospholipase a2. | | 1978 | 620827 |
| amino acid sequence of honeybee prepromelittin synthesized in vitro. | translation of melittin messenger rna from queen bee venom glands in a cell-free system from wheat germ yielded prepromelittin. sequence analysis of the labeled in vitro product was performed by automatic edman degradation of the intact polypeptide as well as by analysis of some of its proteolytic fragments. prepromelittin was shown to be composed of 70 amino acids, two of which have not been identified. the sequence of melittin is located in the cooh-terminal third of the polypeptide chain (res ... | 1978 | 273232 |
| growth, energy and nitrogen budgets and efficiencies of the growing larvae of megachile pacifica (panzer) (hymenoptera: megachilidae). | the larvae of megachile pacifica, the leafcutter bee, develop within a cell constructed of pieces of leaf by the adult female which also provides a plug of nectar and pollen, the sole source of nutrient for the larvae. in this study, eggs (1 per cell) hatched in 2-3 days and larvae were fully grown after a further 9 days at 28° c.the mean larval dry weight (dw) when fully grown (p l ) was 18.89 mg. larvae ate 45.99 mg (dw) (c) of their food leaving 0.90 mg (dw) unconsumed. the faeces (fu) weighe ... | 1978 | 28309132 |
| life history strategy of the honey bee, apis mellifera. | the feral honey bee queens (colonies) of central new york state (usa) show a k-type life history strategy. their demographic characteristics include low early life mortality, low reproductive rate, long lifespan, high population stability and repeated reproductions. identifying the life history strategy of these bees reveals the general pattern of selection for competitive ability, rather than productivity, which has shaped their societies. selection for competitive power explains the adaptivene ... | 1978 | 28308672 |
| fluorescent pigment and pentane production by lipid peroxidation in honey bees, apis mellifera. | | 1978 | 738384 |
| radioprotective potential and chelating properties of glycylhistamine, and analog of histamine terminal peptides found in bee venom. | | 1978 | 725963 |
| sensitization following hymenoptera whole body extract therapy. | treatment of an insect-sensitive patient with hymenoptera whole body extracts (wbe) led, on five occasions, to a serum sickness--like syndrome which did not recur after therapy was stopped. the patient was found to be sensitive by skin test, histamine release, and radioallergosorbent test (rast) to both venom and wbe as well as to venomless bee body preparations. subsequent therapy with honeybee and yellow jacket venoms was without sequelae, and after treatment the patient did not react to a sti ... | 1978 | 637904 |
| [effectiveness of physical rehabilitation in myocardial infarct taking into account long-term observation data]. | the article discusses the results of a one-year follow-up of 439 persons who had suffered from macrofocal or transmural myocardial infarction; aneurysm of the heart was suspected in 128 of them. four to six months after recovery, 60.1% of patients with suspected aneurysm of the heart and 69.1% of those with transmural or macrofocal myocardial infarction were engaged in work involving small physical exertion. mortality in the follow-up period after the end of the acute period of the disease was 8 ... | 1978 | 628132 |
| [kinetic properties of an alkaline phosphatase from honeybee intestine determined by a new rigorous algebraic method]. | the hill-parameters : max. velocity (vm), affinity constant (k) and resultant reaction order (n) of an alk. phosphatase (mol. weight 100 to 130,000) from guts of foraging worker-bees, have been calculated according to new algebraic equations allowing a rigorous determination without any extrapolation. the parameters determined from 2 strains of the same race, at 3 years interval, do not differ, and the hill coefficients (n) are significantly lower than 1, namely : n = 0.621 +/- 0.014 (3 detn.) a ... | 1978 | 157198 |
| effects of passive antibody in bee venom anaphylaxis. | twelve patients allergic to honeybees were challenged by injections of bee venom; five responded systemically to the venom, with symptoms ranging from angioedema to respiratory distress. these patients were given intramuscular or intravenous infusions of gamma-globulin obtained from the plasma of hyperimmune beekeepers who had high levels of antibody to an allergen (phospholipase a) in the venom. post-infusion, all five patients tolerated 1.5 to 5 times the venom dose that previously elicited ad ... | 1978 | 75280 |
| [bee-venom allergy]. | the allergy to bee venom is dangerous and life threatening. skin tests and immunotherapy should be done with bee venom extracts. the rast proves to be helpful if blood taking is done within a short period after stinging. in the meantime immunotherapy should be given lifelong and total and specific ige antibodies controlled. in addition we believe that the determination of igg-blocking antibodies is important. | 1977 | 602341 |
| [characterization of some membrane-active components of vespa orientalis venom]. | the molecular weight distribution of the components of giant hornet (vespa orientalis) venom was studied, using gel-filtration on a column with sephadex g-50. the effects of the venom and its constituent fractions on the permeability and stability of artificial bilayer phospholipid membranes, potassium ions release from the erythrocytes and mitochondrial oxidative phosphorylation parameters, as well as on the activity and stability of polyenzymic systems of the mitochondrial respiratroy chain, w ... | 1977 | 597518 |
| [the presence of conjugated noradrenaline in the wax from the honey comb of apis mellifera linne (proceedings)]. | | 1977 | 79374 |
| [mixed "n" type inhibition of alpha-glucosidase activity by glucose in hemolymph of honeybee prenymphs]. | under in vitro inhibition of alpha-glucosidasic activity by glucose in hemolymph of bee prenymphas, the reaction order (n) (predetermined according to the initial natural glycemia) decreases with increasing inhibitor concentration and the affinity constant between enzyme and substrate undergoes lower variations than in other cases where (n) does not change. the study of inhibited molecular forms suggests the possibility of a gradual negative-cooperativity mechanism concerning a dimeric form of t ... | 1977 | 413666 |
| fisher's syndrome in children. | fisher's syndrome is characterized by ophthalmoplegia, ataxia, and areflexia. the clinical course is usually benign with complete recovery. we report a case that developed following a bee sting and review the literature of fisher's syndrome in children. its history, pathogenesis, and relationship to guillain-barré syndrome are also discussed. | 1977 | 917636 |
| [ideas on the part of patients and doctors regarding the adequacy of psychiatric inpatient and outpatient treatment (author's transl)]. | one fixed day the doctors of a psychiatric university clinic were asked what form of therapy would be best for each of their inpatients (n = 164), and whether these patients could leave the inpatient treatment in case the desired ambulant therapy institutions existed. half a year after this inquiry questionnaires were sent to the patients, and 104 replies were received. the opinions of the patients which treatment would have bee best for them were compared the doctors' judgements. both, doctors ... | 1977 | 609667 |
| [allergy to insect stings (author's transl)]. | generalised reactions after an insect bite call for an exact diagnosis: nonspecific general reactions must be distinguished from true allergic reactions. 12 children with allergic reactions to bee and/or wasp stings were investigated. the results of the skin tests and the allergen-specific ige determinations were compared and found to vary distinctly in some cases. in addition to immediate therapy (emergency pharmacy), specific hyposensitization over several years is indicated. observation of th ... | 1977 | 563944 |
| some carbohydrates found in pollen and pollen substitutes are toxic to honey bees. | carbohydrates in some pollen substitutes (galactose, lactose, raffinose, stachyose, glucuronic acid, galacturonic acid, polygalacturonic acid, and pectin) were toxic to caged adult apis mellifera l. these toxins can be diluted to safe levels by sucrose. collected nectar apparently dilutes the toxic sugars in pollen thus permitting assimilation of essential nutrients from pollen. | 1977 | 903829 |
| bee venon and wbe--a sharp dissent. | | 1977 | 903128 |
| bee and wasp stings of the eye. retained intralenticular wasp sting: a case report. | two different stages should be considered in the management of bee and wasp stings to the eye. the first is the acute stage of activity of the specific insect venom on the structures the eye. the reaction of the eye to the particular insult is considered here in regard to the anterior and posterior segments of the eye. the second stage is that of the retained intraocular foreign body, the inert sting. little is known about the reaction of the eye to the presence of chitinous sting and its effect ... | 1977 | 588520 |
| genetic control and development expression of malate dehydrogenase in apis mellifera. | starch gel electrophoresis of extracts of apis mellifera indicates that genetic variability exists for the enzyme cytoplasmic malate dehydrogenase (e.c. 1.1.1.37). analysis of individuals throughout development indicates that the isozyme patterns are identical for larvae and adults and suggests a dimeric structure for the molecule. the isozyme pattern observed in pupae is more complex than that of larvae and adults may be due to an additional pupal-specific mdh gene being expressed or to an epig ... | 1977 | 588235 |
| polistes wasp hypersensitivity: diagnosis by venom-induced release of histamine in vitro. | polistes wasps cause a majority of hymenoptera-induced anaphylactic reactions in texas. using the in vitro release of histamine from basophils of patients allergic to polistes stings, we have studied the cross-reactivity of venoms from three species of polistes wasps as well as the cross-reactivity among polistes, honeybee, and vespula maculifrons (yellow jacket) venoms. venom collected by an extrusion technique from pollistes exclamans, pollistes apachus, and pollistes carolina caused release o ... | 1977 | 71310 |
| removing a honeybee stinger. | | 1977 | 18748046 |
| a high molecular weight allergenic fraction of honeybee venom. | chromatography of honeybee venom on sephadex g-150 super fine revealed a high molecular weight (hmw) fraction that elutes prior to hyaluronidase (hyal) and comprises 2% to 4% of the venom weight. hmw appears to exist in polymeric form, and the polymer which is present in greatest concentration has an estimated molecular weight of 105,000 d. the 12% nitrogen content of hmw suggests it may not be all protein. hmw is antigenically and enzymatically distinct from hyal and phospholipase a2 (phos a). ... | 1977 | 70436 |
| phospholipases: melittin facilitation of bee venom phospholipase a2-catalyzed hydrolysis of unsonicated lecithin liposomes. | | 1977 | 20842 |
| ethological study of african carpenter bees of the genus xylocopa (hymenoptera, anthophoridae). | over 200 bees of 4 african xylocopa species were observed for 3 months on the island of rubondo (l. victoria, tanzania). some 40 burrows were investigated, 100 bees marked. building techniques are minutely reported; burrow construction simplifies defence and allows re-use by succeeding generations. food plants, collecting, provisioning and all aspects of ontogenesis are treated, insight given into pupal leg mobility and the much-debated emergence order after eclosion: the first-hatched bee, in t ... | 1977 | 563151 |
| the treatment of bee stings. | | 1977 | 331296 |
| [determination of the kinetic parameters of enzyme inhibition of the k-type mechanism: application to the control of alpha-glucosidase activity of honeybee hemolymph by glucose]. | the alpha-glucosidasic activity of emerging honeybees haemolymph is submitted to a feed-back inhibition by glucose, according to a mechanism of the "k" type (competitive). the "resulting affinity-constant" (measured in the presence of the enzyme both with substrate and inhibitor) is linear function of the inhibitor concentration. the affinity constants between enzyme and pure substrate on one hand, and between enzyme and pure inhibitor on the other hand, were determined by means of this relation ... | 1977 | 409564 |
| separation and detection of phencyclidine in urine by gas chromatography. | a rapid, sensitive and selective analytical procedure for phencyclidine and one of its metabolites in urine has bee developed. three techniques have been studied for extraction of the drug from the biological matrix: (a) reversed-phase xad resin, (b) charcoal absorption, and (c) solvent extraction using chloroform. temperature-programmed gas chromatography was used to quantitate the illicit drug. solvent extraction appears to offer the most efficient separation of the drug and its metabolite, as ... | 1977 | 893578 |
| [modification of alpha-glucosidases (e.c. 3.2.1.20) activity in honeybee hemolymph during nymphosis]. | the main alpha-glucosidase fraction of honey-bee hemolymph undergoes an important increase of activity for a short time, at a precise stage of nymphosis (pink-brown eyes), without any changes of the apparent-michaelis constant, nor corresponding increase of the protein quantity. | 1977 | 409545 |
| the mycoflora of adult worker honeybees, apis mellifera: effects of 2,4,5-t and caging of bee colonies. | | 1977 | 925366 |
| [allergic reactions in patients with wasp and bee stings]. | | 1977 | 898868 |
| estimation of the number of sex alleles and queen matings from diploid male frequencies in a population of apis mellifera. | the distribution of diploid males in a population of apis mellifera was obtained by direct examination of the sexual phenotypes of the larvae. using these data, estimates are derived for the number of sex alleles and the number or matings undergone by the queen. the number of sex alleles is estimated to be 18.9. the estimate is larger than previous ones, which have ranged between 10 and 12. however, the increase in the number of sex alleles can be explained by the large effective population numb ... | 1977 | 892423 |
| concept of internal structural controls for evaluation of inactive synthetic peptide analogs: synthesis of [orn13,14]apamin and its guanidination to an apamin derivative with full neurotoxic activity. | the importance of arginine residues 13 and 14 in the bee venom neurotoxin, apamin, was teste by the synthesis of replacement analogs. [13,14-di-ndelta-trifluoroacetylornithine]apamin was synthesized by the solid phase method on a benzhydrylamine resin. it was deprotected to [13,14-diornithine]apamin, which was then guanidinated to produce the 4-homoarginine-13,14-diarginine analog, [har4]apamin. neither the trifluoroacetylornithine analog nor the ornithine analog produced any detectable symptoms ... | 1977 | 268626 |
| [value of total thyroidectomy with cervical dissection in the treatment of differentiated thyroid cancers]. | study of 52 cases of differentiated carcinoma of the thyroid treated by anatomically total thyroidectomy with block dissection of the cervical nodes indicated three amin findings: examination by puncture for cytological examination would have missed 8 micro-carcinomas; unilateral excistion would have bee inadequate in 12 patients; lymph node excision confined to detectable lymphadenopathies would have left neoplastic tissue in place in 8 cases. in the light of these advantages from an oncologica ... | 1977 | 887418 |
| succinyl bee venom melittin is a leukocyte chemotactic factor. | | 1977 | 876392 |
| allergy to bee venom: in vitro diagnosis and identification and isolation of allergens. | | 1977 | 872619 |
| [treatment by bee stings]. | | 1977 | 587841 |
| incorporation into lipid bilayer membranes of a photo--sensitive pigment from the honeybee compound eye. | an increase of electrical conductance up to a factor 10(2)--5-10(2) was obtained by adding, in the dark, the honeybee photopigment to a positively charged lipid bilayer. the increase in conductance was made slower by illuminating the system during the incorporation of the protein into the membrane and it was negligible when the photopigment was bleached before the incorporation. the interaction of the photopigment with the membrane is tentatively interpreted in terms of formation of channels. | 1977 | 861223 |
| [specific hyposensitization in patients with allergic reaction to bee and wasp bites]. | | 1977 | 875344 |
| allergens in hymenoptera venoms. iv. comparison of venom and venom sac extracts. | honeybee venom sac extract is compared with pure venom. all five known allergens of venom are present in venom sac extract. enzyme analyses indicate that the sac extracts contain 11% to 16% venom. at least 10 additional components, several of which are proteins, are present in venom sac extract. radioallergosorbent test (rast) studies of yellow jacket venom and venom sac extract yielded a correlation of r = 0.94, with only some weakly reactive sera positive to only one preparation. a few sera we ... | 1977 | 856902 |
| allergens in bee venom. iii. identification of allergen b of bee venom as an acid phosphatase. | allergen b previously isolated from honeybee venom and shown to be a mildly acidic protein consisting of polymers of a chain of 49,000 d is shown to have acid phosphatase activity. allergen b is homogeneous by several criteria. no acid phosphatase, alkaline phosphatase, or esterase activity was found in any other allergen or fraction of bee venom. acid phosphatase activity was also found in yellow jacket venom and extracts of venom sacs from bumblebees and paper wasps. | 1977 | 853176 |
| serological investigations in hymenoptera sting allergy: ige and haemagglutinating antibodies against bee venom in patients with bee sting allergy, bee keepers and non-allergic blood donors. | specific ige-antibodies and haemagglutinins to bee venom were determined in fifty-five bee sting allergic patients, fifty-seven bee keepers and fifty-two blood donors without evident allergy to hymenoptera stings. ige-antibodies were detected by rast in 70% of allergic patients, 40% of bee keepers and 12% of blood donors. most bee keepers with detectable ige-antibodies to bee venom recorded severe local or even general reactions to bee stings. most blood donors with detectable specific ige had b ... | 1977 | 872361 |
| [basic peptides in bee venom, iii. synthesis of peptide fragments from the sequence of the mast-cell-degranulating peptide (author's transl)]. | the synthesis by conventional methods of the following three peptides is described: mcd(8-11) boc-his(trt)-val-ile-lys(z) (iii) mcd(5-7) boc-cys(sipr)-lys(z)-arg(tos) (iv) and mcd(1-4) boc-ile-lys(z)-cys(trt)-asn(mbh) (v). these peptides are fragments of the mast cell degranulating peptide from bee venom. the purity of the fragments synthesized was examined by thin-layer chromatography, amino acid and elementary analysis. including the fragments boc-lys(z)-ile-cys(sipr)-gly-lys(z) (i) and boc-pr ... | 1977 | 856709 |