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the effect of piper aduncum linn. (family: piperaceae) essential oil as aerosol spray against aedes aegypti (l.) and aedes albopictus skuse.the bioefficacy of piper aduncum l. essential oil formulated in aerosol cans was evaluated against aedes aegypti and aedes albopictus in a simulated room. the aerosol spray test was based on the malaysian test standard for aerosol (ms 1221:1991udc 632.982.2 modified from who 2009 methodology) and examined the knockdown effect within 20 minutes of exposure. mortality rate after 24 hour of holding period was also determined. a commercial aerosol spray (0.09% prallethrin 0.05% d-phenothrin) was als ...201122041743
development and evaluation of a single-step multiplex pcr to differentiate the aquatic stages of morphologically similar aedes (subgenus: stegomyia) species.to develop a single-step multiplex pcr to differentiate the aquatic stages of aedes aegypti, aedes albopictus and aedes vittatus collected from different breeding spots in arbovirus endemic/epidemic areas and to detect the most abundant species by the multiplex pcr.201222040518
field performance of engineered male mosquitoes.dengue is the most medically important arthropod-borne viral disease, with 50-100 million cases reported annually worldwide. as no licensed vaccine or dedicated therapy exists for dengue, the most promising strategies to control the disease involve targeting the predominant mosquito vector, aedes aegypti. however, the current methods to do this are inadequate. various approaches involving genetically engineered mosquitoes have been proposed, including the release of transgenic sterile males. how ...201122037376
[knowledge, attitudes and community practice during an outbreak of dengue in a town in colombia, 2010].17 cases of dengue were reported from the town of el playón (7°28'15"-n/73°12'00"-w/altitude 510 masl) during epidemiological study weeks 5-6 and 12-13/2010. the santander department's health secretariat sought outbreak investigation and community knowledge-attitudes-practice (kap) assessment on 16-4-2010 concerning a dengue fever outbreak in the town after an epidemiological surveillance committee had met; this was to strengthen local action regarding aedes aegypti vector promotion-prevention-c ...201122031003
[a mathematical model for the chemical control of aedes aegypti (diptera: culicidae) having acquired chemical resistance].dengue fever is a common vector-borne disease in tropical and subtropical areas. it is transmitted to humans by the bite of an infected female aedes mosquito. since no vaccines are currently available which can protect against infection, disease control relies on controlling the mosquito population. this work was aimed at modelling such mosquito's population dynamics regarding chemical control of the adult population and its acquired resistance to chemicals. the model was analysed by using class ...201022030690
aedes aegypti susceptibility to insecticide from abidjan city, cote d'ivoire.the susceptibility of aedes aegypti adults of three places in abidjan city selected for an entomological surveillance of potential arbovirus vectors to permethrin, deltamethrin, lambdacyhalothrin, and propoxur was determined using who standard procedures. the wild populations of a. aegypti were susceptible to permethrin, deltamethirn, and lambdacyhalothin. resistance to propoxur was detected in strains collected at the autonomous port of abidjan and at koumassi (mortality rate: 77%) but possibly ...201222029425
phylogeography of aedes aegypti in argentina: long-distance colonization and rapid restoration of fragmented relicts after a continental control campaign.aedes (stegomyia) aegypti, the main vector of dengue and yellow fever viruses, is present in all the northern and central provinces of argentina. during 2009, a dengue outbreak spread broadly throughout the country, causing 27,752 infections in 13 provinces. in argentina, little is known about the demographic history of this vector, which suffered a drastic decrease in abundance and distribution during a major control campaign performed in the americas between 1950 and 1960. with the aim of unco ...201222022812
green synthesis of silver nanoparticles for the control of mosquito vectors of malaria, filariasis, and dengue.a biological method was used to synthesize stable silver nanoparticles that were tested as mosquito larvicides against aedes aegypti, anopheles stephensi, and culex quinquefasciatus. annona squamosa leaf broth (5%) reduced aqueous 1 mm agno₃ to stable silver nanoparticles with an average size of 450 nm. the structure and percentage of synthesized nanoparticles was characterized by using ultraviolet spectrophotometry, x-ray diffraction, fourier transform infrared spectroscopy, and scanning electr ...201222022807
mosquito aedes aegypti (l.) leucokinin receptor is critical for in vivo fluid excretion post blood feeding.the evolution of the blood feeding adaptation in mosquitoes required hormonal coordination of multiple physiological processes (behavior, digestion, diuresis, oogenesis). the aedes kinins (leucokinin-like neuropeptides) are involved in post blood feeding physiological processes, having diuretic and myotropic functions. to understand the in vivo contribution of the kinin receptor to overall female post-prandial fluid excretion, rnai knockdown was followed by fluid secretion assays which proved it ...201122019456
ground application of aquareslin and aquakontrol against anopheles quadrimaculatus, aedes aegypti, and aedes albopictus.two permethrin formulations, aquareslin and aquakontrol, were applied by ultra-low volume truck-mounted sprayers on test plots under appropriate meteorological condition at jacksonville, fl. the method of application was followed from the product label. the application rate for each formulation was 0.79 g permethrin per acre. aquareslin and aquakontrol demonstrated similar results against anopheles quadrimaculatus; the former was superior to the latter used against aedes aegypti, and ae. albopic ...201122017102
biosynthesis of silver nanoparticles by using mangrove plant extract and their potential mosquito larvicidal property.to identify the larvicidal activities of silver nano particles synthesised with rhizophora mucronata (r. mucronata) leaf extract against the larvae of aedes aegypti (ae. aegypti) and culex quinquefasciatus (cx. quinquefasciatus).201122014736
threat of dengue fever and dengue haemorrhagic fever to egypt from travelers.dengue (df) and dengue hemorrhagic fevers (dhf) are present in urban and suburban areas in the americas, south-east asia, the eastern mediterranean and the western pacific, but dengue fever is present mainly in the rural areas of africa. several factors have combined to produce epidemiological conditions in developing countries in the tropics and subtropics that favour viral transmission by the main mosquito vector, aedes aegypti as the rapid population growth, rural-urban migration, inadequate ...201121980768
larvicidal and repellent activity of tetradecanoic acid against aedes aegypti (linn.) and culex quinquefasciatus (say.) (diptera:culicidae).to investigate the larvicidal and repellent efficacy of tetradecanoic acid against aedes aegypti (ae. aegypti) l. and culex quinquefasciatus (cx. quinquefasciatus) say (diptera: culicidae).201121967693
brain development in the yellow fever mosquito aedes aegypti: a comparative immunocytochemical analysis using cross-reacting antibodies from drosophila melanogaster.considerable effort has been directed towards understanding the organization and function of peripheral and central nervous system of disease vector mosquitoes such as aedes aegypti. to date, all of these investigations have been carried out on adults but none of the studies addressed the development of the nervous system during the larval and pupal stages in mosquitoes. here, we first screen a set of 30 antibodies, which have been used to study brain development in drosophila, and identify 13 o ...201121956584
changes in the fat body during the post-embryonic development of the predator toxorhynchites theobaldi (dyar & knab) (diptera: culicidae).several studies have focused on understanding the biochemistry and morphology of the fat body of the hematophagous mosquito aedes aegypti (l.) (diptera: culicidae). in contrast, few studies, if any, have focused on morphological characters of the fat body in other mosquitoes, especially non-hematophagous taxa such as the culicid toxorhynchites. larvae of toxorhynchites prey upon the larvae of other mosquito species and are used in vector mosquito control. we investigated aspects of the fat body ...201121952962
mosquitocidal and water purification properties of cynodon dactylon, aloe vera, hemidesmus indicus and coleus amboinicus leaf extracts against the mosquito vectors.ethanolic extracts of cynodon dactylon, aloe vera, hemidesmus indicus and coleus amboinicus were tested for their toxicity effect on the third-instar larvae of anopheles stephensi, culex quinquefasciatus and aedes aegypti. the leaves of c. dactylon, a. vera, h. indicus and c. amboinicus were collected from natural habitats (forests) in western ghats, tamil nadu, india. a total of 250 g of fresh, mature leaves were rinsed with distilled water and dried in shade. the dried leaves were put in soxhl ...201221947308
molecular characterization of genes encoding trypsin-like enzymes from aedes aegypti larvae and identification of digestive enzymes.trypsin-like enzymes play an important role in the aedes aegypti digestive process. the trypsin-like enzymes present in adults were characterized previously, but little is known about trypsins in larvae. in the present work, we identified one of the trypsin enzymes from ae. aegypti larval midgut using a library of trypsin gene fragments, which was the sequence known as aael005607 from the ae. aegypti genome. quantitative pcr analysis showed that aael005607 was transcribed in all larval instars, ...201121914468
costs of dengue prevention and incremental cost of dengue outbreak control in guantanamo, cuba.to assess the economic cost of routine aedes aegypti control in an at-risk environment without dengue endemicity and the incremental costs incurred during a sporadic outbreak.201221906216
a transient increase in total head phosphotyrosine levels is observed upon the emergence of aedes aegypti from the pupal stage.phosphorylation and dephosphorylation of protein tyrosine residues constitutes a major biochemical regulatory mechanism for the cell. we report a transient increase in the total tyrosine phosphorylation of the aedes aegypti head during the first days after emergence from the pupal stage. this correlates with an initial reduction in total head protein tyrosine phosphatase (ptp) activity. similarly, phosphotyrosine (ptyr)-containing bands are seen in extracts prepared from both male and female hea ...201121894374
comparative larvicidal potential of different plant parts of withania somnifera against vector mosquitoes in the semi-arid region of rajasthan.larvicidal potential of the extracts from different parts viz. green and red fruits, seeds, fruit without seeds, leaves and roots of withania somnifera in different solvents was evaluated against larvae of anopheles stephensi, aedes aegypti and culex quinquefasciatus, the important disease vectors prevalent in the semi-arid region. experiments were carried out on late 3rd or early 4th instar larvae of these mosquitoes using standard who technique. 24 and 48 hr lc50 values along with their 95% co ...201121888235
biological activity of selected lamiaceae and zingiberaceae plant essential oils against the dengue vector aedes aegypti l. (diptera: culicidae).the larvicidal activity of hydrodistillate extracts from mentha piperita l. ocimum basilicum l. curcuma longa l. and zingiber officinale l. were investigated against the dengue vector aedes aegypti l. (diptera: culicidae).the results indicated that the mortality rates at 80, 100, 200 and 400 ppm of m. piperita, z. officinale, c. longa and o. basilicum concentrations were highest amongst all concentrations of the crude extracts tested against all the larval instars and pupae of a. aegypti. result ...201221881945
prevalence of aedes aegypti linnaeus and aedes albopictus skuse in koderma, jharkhand.entomological survey was carried out in different localities of koderma district of jharkhand with a view to study the prevalence, distribution and stratification of areas for aedes mosquito species. a total of 233 houses were covered during house to house larval and adult survey. aedes breeding could be detected in 157 houses. in all, a total of 942 domestic water containers were searched, out of which 461 were found positive. the overall house index(hi) container index(ci) breteau index(b1) an ...201123781636
epidemiological significance of the breeding of mosquitoes in discarded automobile tyres in zaria, northern nigeria.waste automobile tyres are suitable receptacles of rainwater thus capable of supporting the breeding of vector mosquito species in close proximity to humans and altering disease transmission risks. bionomics of mosquitoes in carelessly discarded tyres was explored to unravel species composition and abundance, physicochemical parameters of microhabitats and epidemiological significance. of the 1179 larvae isolated from 90/212 (42.45%) positive tyres were ten species belonging to aedes and culex g ...201123781631
[convalescence of pregnant women who suffered from dengue serotype 3].dengue is an infectious disease caused by arn genome virus and has 4 recognized serotypes (den-1, den-2, den-3 and den-4) that are transmitted by aedes aegypti as the main vector. the clinical manifestations of dengue in pregnancy are similar to those of the general population, except that the ecosystem is made up by the mother and the fetus where the virus with its genetic characteristics is introduced and finds special conditions for development and evolution.201323444608
daily oviposition activity of aedes aegypti in orán, argentina.the study aimed to determinate the maximum daily peak of aedes aegypti oviposition in the city of oran, northwestern argentina. biweekly samplings were taken between november 2006 and february 2007 (spring-summer). the city was divided into three areas (north, center, and south) and households were randomly selected. two ovitraps were placed outdoors in the selected houses. ovitraps were replaced every four hours, from morning (8 a.m.) to late afternoon (8 p.m.). the largest number of eggs was r ...201121860910
larvicidal activity of copaifera sp. (leguminosae) oleoresin microcapsules against aedes aegypti (diptera: culicidae) larvae.studies have demonstrated the potential of copaifera sp. oleoresin to control aedes aegypti proliferation. however, the low water solubility is a factor that limits its applicability. thus, the micro- or nanoencapsulation could be an alternative to allow its use in larval breeding places. the purpose of this study was to evaluate if achievable lethal concentrations could be obtained from copaifera sp. oleoresin incorporated into polymers (synthetic or natural) and, mainly, if it can be sustained ...201221850452
identification of mosquito biting deterrent constituents from the indian folk remedy plant jatropha curcas.an investigation of the indian folk remedy plant jatropha curcas l., was performed to identify the constituents responsible for the mosquito biting deterrent activity of the oil. j. curcas seed oil is burned in oil lamps in india and parts of africa to repel biting insects, primarily mosquitoes. the seed oil was thoroughly analyzed by 1h nmr, 13c nmr, high-performance liquid chromatography-refractive index, and gas chromatography-flame ionization detection to identify the constituents in the oil ...201121845943
[spatial interpolation of aedes aegypti larvae abundance for locating infestation foci].design and implement a surveillance method for locating aedes aegypti infestation foci with the use of larvae traps and spatial interpolation techniques, which facilitate the ongoing estimation of vector abundance in the area by counting the individuals collected in the study area.201121829965
chemical composition and antioxidant, antimicrobial, and larvicidal activities of the essential oils of annona salzmannii and a. pickelii (annonaceae).the essential oils from the leaves of annona salzmannii and a. pickelii (annonaceae) growing in sergipe, northeastern region of brazil, were obtained by hydrodistillation using a clevenger-type apparatus, and analyzed by gc/ms and gc/fid. thirty-four compounds were identified in the essential oil of a. salzmannii and twenty-seven in that of a. pickelii; sesquiterpenes predominated in both essential oils. bicyclogermacrene (20.3%), (e)-caryophyllene (19.9%), delta-cadinene (15.3%), alpha-copaene ...201121815437
efficacy evaluation of a fumigant containing permethrin and deltamethrin against vector mosquitoes.the efficacy of a new smoke-generating formulation (fumigant), containing the active ingredient permethrin (69 g/kg) and deltamethrin (3.9 g/kg) per cartridge, was evaluated against adult female anopheles albimanus, aedes aegypti, and culex quinquefasciatus in a large vehicle storage building (22 x 13 x 4.5 m3), florida, u.s.a., and against cx. pipiens complex and ae. albopictus in a furnished apartment (12 x 7 x 3.5 m3) in israel. in the storage building, the use of 2 fumigant cartridges result ...201121805854
larvicidal activity of tagetes erecta against aedes aegypti.the aim of this study was to evaluate the activity of essential oil from tagetes erecta against 3rd instars of aedes aegypti and to determine the amounts of larvicidal thiophenes in all plant tissues. the oil obtained by steam distillation and analyzed by gas chromatography/mass spectrometry showed 14 compounds. the main compounds were piperitone (45.72%), d-limonene (9.67%), and piperitenone (5.89%). the essential oil was active against larvae of ae. aegypti, with lc50 of 79.78 microg/ml and lc ...201121805850
effects of temperature and diet on development and interspecies competition in aedes aegypti and aedes albopictus.we asked whether climate change might affect the geographic distributions of aedes aegypti (l.) and aedes albopictus (skuse) (diptera: culicidae). we tested the effects of temperature, diet and the presence of congeneric species on the performance of immature stages of these two aedine species in the laboratory. mosquitoes in three different species-density combinations were reared at four constant temperatures (20 °c, 25 °c, 30 °c, 35 °c) on low- or high-level diets. of the four temperatures te ...201221781139
mosquito larvicidal and ovicidal activity of cardiospermum halicacabum linn. (family: sapindaceae) leaf extract against culex quinquefasciatus (say.) and aedes aegypti (linn.) (diptera: culicidae).to investigate the larvicidal and ovicidal efficacy of different extracts of cardiospermum halicacabum l. against culex quinquefasciatus say and aedes aegypti l. (diptera: culicidae).201121780548
tree holes as larval habitats for aedes aegypti in public areas in aguaray, salta province, argentina. 201121780333
deltamethrin: promising mosquito control agent against adult stage of aedes aegypti l.to evaluate the effects of deltamethrin against field-collected adults of aedes aegypti l. (ae. aegypti).201121771693
evaluation of andrographis paniculata burm.f. (family:acanthaceae) extracts against culex quinquefasciatus (say.) and aedes aegypti (linn.) (diptera:culicidae).to investigate the larvicidal and ovicidal efficacy of different extracts of andrographis paniculata (a. paniculata) against culex quinquefasciatus (cx. quinquefasciatus) say and aedes aegypti (ae. aegypti) l. (diptera: culicidae).201121771448
mosquito larvicidal and ovicidal properties of eclipta alba (l.) hassk (asteraceae) against chikungunya vector, aedes aegypti (linn.) (diptera: culicidae).the present study deals with the investigation of larvicidal and ovicidal activities of benzene, hexane, ethyl acetate, methanol and chloroform leaf extract of eclipta alba (e. alba) against dengue vector, aedes aegypti (ae. aegypti).201121771410
evaluation of time toxicity, residual effect, and growth-inhibiting property of carapa guianensis and copaifera sp. in aedes aegypti.oils of carapa guianensis and copaifera spp. are well-known in the amazonian region as natural insect repellents, and studies have reported their efficiency as larvicide against some mosquito species. however, toxicity persistence and effect on mosquito development have not yet been evaluated. thus, the objective of this study was to evaluate the initial time of larvicidal activity, residual effect, and the effect of very low concentrations of these oils on aedes aegypti. different concentration ...201221766235
mosquito larvicidal activity of methyl-p-hydroxybenzoate isolated from the leaves of vitex trifolia linn.the vector-borne diseases caused by mosquitoes are one of the major health problems in many countries especially in tropical and sub-tropical countries. the resistance of mosquitoes to synthetic chemicals and environmental toxicity created by the chemicals raised the demand for finding of alternate natural molecules that control mosquito. in the present study, a crystalline compound methyl-p-hydroxybenzoate was isolated from the methanol extract of vitex trifolia leaves and it was identified by ...201221763671
bioefficacy of larvicdial and pupicidal properties of carica papaya (caricaceae) leaf extract and bacterial insecticide, spinosad, against chikungunya vector, aedes aegypti (diptera: culicidae).the present study was carried out to establish the properties of carica papaya leaf extract and bacterial insecticide, spinosad on larvicidal and pupicidal activity against the chikungunya vector, aedes aegypti. the medicinal plants were collected from the area around bharathiar university, coimbatore, india. c. papaya leaf was washed with tap water and shade-dried at room temperature. an electrical blender powdered the dried plant materials (leaves). the powder (500 g) of the leaf was extracted ...201221750871
evaluation of larvicidal activity of acalypha alnifolia klein ex willd. (euphorbiaceae) leaf extract against the malarial vector, anopheles stephensi, dengue vector, aedes aegypti and bancroftian filariasis vector, culex quinquefasciatus (diptera: culicidae).the leaf extract of acalypha alnifolia with different solvents - hexane, chloroform, ethyl acetate, acetone and methanol - were tested for larvicidal activity against three important mosquitoes such as malarial vector, anopheles stephensi, dengue vector, aedes aegypti and bancroftian filariasis vector, culex quinquefasciatus. the medicinal plants were collected from the area around kallar hills near the western ghats, coimbatore, india. a. alnifolia plant was washed with tap water and shade drie ...201221748350
[behavior of immatures aedes aegypti in the coast state of são paulo, brazil].in a region of high dengue incidence, on the coast of the state of são paulo, 9 areas were selected to evaluate the behavior of immature aedes aegypti.201121739074
effect of myracrodruon urundeuva leaf lectin on survival and digestive enzymes of aedes aegypti larvae.aedes aegypti transmits the viruses that cause yellow and dengue fevers. vector control is essential, since a vaccine for dengue has not as yet been made available. this work reports on the larvicidal activity of myracrodruon urundeuva leaf lectin (mull) against a. aegypti fourth-stage larvae (l(4)). also, the resistance of mull to digestion by l(4) gut proteases and the effects of mull on protease, trypsin-like and α-amylase activities from l(4) gut were evaluated to determine if lectin remains ...201221735148
enantiomeric selectivity in behavioural and electrophysiological responses of aedes aegypti and culex quinquefasciatus mosquitoes.1-octen-3-ol is a kairomone for many haematophagous insects including mosquitoes. numerous studies have examined the effects of racemic 1-octen-3-ol; however, few studies have investigated the role of individual enantiomers in relation to mosquito attraction. in the present study, we investigated the behavioural and electrophysiological responses of two mosquito species, aedes aegypti and culex quinquefasciatus, to individual enantiomers and mixtures of 1-octen-3-ol, employing a laboratory y-tub ...201121729394
droplet size and efficacy of an adulticide-larvicide ultralow-volume formulation on aedes aegypti using different solvents and spray application methods.when cases of dengue are reported or the density of adult aedes aegypti (l.) becomes too high, ultralow-volume (ulv) application of insecticides is the recommended control method. the droplet size of an aerosol insecticide influences its efficiency in killing adult mosquitoes. many studies have been carried out to determine the optimum droplet size that maximises vector control efforts, but only a few have determined droplet-size spectra for specific equipment using different solvents and compar ...201221714060
antioxidative systems defense against oxidative stress induced by blood meal in aedes aegypti.the release of iron from hemoglobin via the digestion of a blood meal in female mosquitoes can potentially induce oxidative damage and even death. these mosquitoes need an effective antioxidant to prevent this. we carried out this study to determine the antioxidant activities of ferritin, glutathione peroxidase (gpx), glutathione s-transferase (gst) and catalase, and glutathione (gsh). these enzymes had their greatest activity among 4 day old virgin female mosquitoes. using a single blood feed m ...201121706932
is routine dengue vector surveillance in central brazil able to accurately monitor the aedes aegypti population? results from a pupal productivity survey.to assess how well the aedes aegypti infestation rapid survey, levantamento rapido de indice para aedes aegypti (liraa), is able to accurately estimate dengue vector densities and target the most important breeding sites in goiania, brazil.201121702871
composition of the essential oil constituents from leaves and stems of korean coriandrum sativum and their immunotoxicity activity on the aedes aegypti l.the leaves and stems of coriandrum sativum were extracted and the essential oil composition and immunotoxicity effects were studied. the analyses were conducted by gas chromatography-mass spectroscopy (gc-ms), which revealed the essential oils of c. sativum leaves and stems. thirty-nine components representing 99.62% of the total oil were identified from the leaves. the major components are cyclododecanol (23.11%), tetradecanal (17.86%), 2-dodecenal (9.93%), 1-decanol (7.24%), 13-tetradecenal (6 ...201221692682
compatible interaction with its rice host leads to enhanced expression of the gamma subunit of oligosaccharyl transferase in the asian rice gall midge, orseolia oryzae.the asian rice gall midge, orseolia oryzae, is a fast evolving, damaging pest of rice. understanding the underlying molecular mechanism of interaction between the gall midge and rice will help in devising strategies to control and manage the pest. the present study aims to identify rice-responsive genes in the gall midge that aid pest survival. the abundance of transcripts coding for enzymes related to glycosylation, in a cdna library prepared from maggots of the rice gall midge feeding on susce ...201121668550
productive container types for aedes aegypti immatures in mérida, méxico.during 2007-2010, we examined which container types in mérida, méxico, are most productive for aedes aegypti (l.) immatures. surveys for mosquito immatures followed routine surveillance methodology and container type classifications used by servicios de salud de yucatán. our main findings were that (1) small and larger discarded containers that serve no particular purpose and therefore can be removed from the environment contribute strongly to larval and pupal production in mérida, and (2) the i ...201121661326
changes in host-seeking behavior of puerto rican aedes aegypti after colonization.the effects of colonization on host-seeking behavior of mosquitoes was examined by comparing attraction responses of newly colonized aedes aegypti (l.) from field-collected eggs in puerto rico to that of the gainesville (florida) strain, originally from orlando (florida) and in colony since 1952. females from the orlando and the f0 through f10 generations of the puerto rico strain were evaluated using attractant odors in a triple-cage dual-port olfactometer. two attractant sources were used: odo ...201121661313
why ridl is not sit.history teaches that sterile insect technique (sit) is a feasible strategy for mosquito population suppression. female killing (fk) technologies developed later theoretically had greater potential than sit, but depended upon chromosomal translocations. unfortunately these were genetically unstable. new transgenic strategies have been misinterpreted as a replacement for sit. instead these strategies provide a means to revisit fk. conditional lethal mutations inserted into mosquito genomes allow f ...201121659002
risk of dengue occurrence based on the capture of gravid aedes aegypti females using mosquitrap.we assessed the risk classification of dengue fever based on the capture of aedes aegypti adults using mosquitrap, a type of sticky trap, in comparison with traditional larval infestation indices. a total of 27 mosquitraps were installed, with one trap per block, and were inspected weekly between november 2008-february 2009. infestation baseline data were obtained from a survey conducted prior to trap installation. the index generated by mosquitrap and house index (hi) classified the area "in al ...201121655827
multiple insecticide resistance in aedes aegypti (diptera: culicidae) populations compromises the effectiveness of dengue vector control in french guiana.in french guiana, pyrethroids and organophosphates have been used for many years against aedes aegypti. we aimed to establish both the resistance level of ae. aegypti and the ultra low volume spray efficacy to provide mosquito control services with practical information to implement vector control and resistance management. resistance to deltamethrin and fenitrothion was observed. in addition, the profound loss of efficacy of aquak'othrine® and the moderate loss of efficacy of paluthion® 500 wer ...201121655824
insights into the transcriptome of oenocytes from aedes aegypti pupae.oenocytes are ectodermic cells present in the fat body of several insect species and these cells are considered to be analogous to the mammalian liver, based on their role in lipid storage, metabolism and secretion. although oenocytes were identified over a century ago, little is known about their messenger rna expression profiles. in this study, we investigated the transcriptome of aedes aegypti oenocytes. we constructed a cdna library from ae. aegypti moyo-r strain oenocytes collected from pup ...201121655818
evaluation of culture filtrates of culicinomyces clavisporus: mycoadulticide for culex quinquefasciatus, aedes aegypti and anopheles stephensi.the culicinomyces clavisporus is a fungal pathogen of a wide range of mosquito larvae. the c. clavisporus was isolated from the larvae of culiseta inornata. we have investigated into potential pathogenicity against the adults of culex quinquefasciatus, aedes aegypti and anopheles stephensi. the culture filtrates released from the strain of c. clavisporus 46258 were grown in the emypss broth, were filtered and used for the bioassays after a growth of 15 days. the results demonstrated these metabo ...201221647670
frequency of pyrethroid resistance in aedes aegypti and aedes albopictus (diptera: culicidae) in thailand.thirty-two aedes aegypti populations collected throughout thailand and five populations of aedes albopictus from southern thailand were subjected to standard who contact bioassays to assess susceptibility to three commonly used synthetic pyrethroids: permethrin, deltamethrin, and lambda-cyhalothrin. a wide degree of physiological response to permethrin was detected in ae. aegypti, ranging from 56.5% survival (lampang, northern thailand) to only 4% (kalasin in northeastern and phuket in southern ...201121635659
heteropteran insects as mosquito predators in water jars in southern vietnam.residents of vietnam living in areas with water shortages and/or poor tap water maintain water storage containers, such as jars, in and around their domiciles in order to store water used in daily life. although these water jars are known to be important breeding sources of the aedes mosquito, use of chemical larvicides in such containers is legally prohibited in vietnam. in this study, we identified the dominant mosquito insect predators in water jars in and around residences located in tan cha ...201121635655
cold season mortality and hatching behavior of aedes aegypti l. (diptera: culicidae) eggs in buenos aires city, argentina.in temperate regions, populations of aedes aegypti survive the cold season in the egg stage. in the present work, we studied the cold-season mortality of ae. aegypti eggs and their subsequent hatching pattern in buenos aires city. eggs were exposed during the winter season (three months) in three neighborhoods located along a gradient of distance toward the río de la plata river, coincident with a gradient of activity of ae. aegypti. results showed mortalities lower (30.6%) than those from tropi ...201121635646
abundance and prevalence of aedes aegypti immatures and relationships with household water storage in rural areas in southern viet nam.since 2000, the government of viet nam has committed to provide rural communities with increased access to safe water through a variety of household water supply schemes (wells, ferrocement tanks and jars) and piped water schemes. one possible, unintended consequence of these schemes is the concomitant increase in water containers that may serve as habitats for dengue mosquito immatures, principally aedes aegypti. to assess these possible impacts we undertook detailed household surveys of ae. ae ...201124038184
does targeting key-containers effectively reduce aedes aegypti population density?the elimination of aedes aegypti breeding sites has been broadly adopted worldwide to keep vector population density below a critical threshold. we observed the effectiveness of targeting the most productive containers on adult a. aegypti females density, which was evaluated weekly.201121605290
effects of natal habitat odour, reinforced by adult experience, on choice of oviposition site in the mosquito aedes aegypti.the effects of natal experience on the oviposition behaviour of adult female mosquitoes were investigated in the laboratory using aedes aegypti (l.) (diptera: culicidae). 'treatment' mosquitoes were exposed to a dilute repellent (inducing stimulus) in their breeding water (aquatic stages) and/or in the air (adults) during various combinations of life stages [larval only (l regime); larval and pupal (lp regime); larval, pupal and emergent adult (lpe regime); larval, pupal, emergent adult and adul ...201121605148
ovitrap surveillance of the dengue vectors, aedes (stegomyia) aegypti (l.) and aedes (stegomyia) albopictus skuse in selected areas in bentong, pahang, malaysia.ovitrap surveillance was conducted in methodically selected areas in bentong, pahang, malaysia from june 2008 till december 2009 in order to identify insular sites with stable aedes aegypti population. eleven sites were surveyed in bentong district, pahang, and one of these locations (n3º33' e101º54') was found to have an ovitrap index of ae. aegypti and aedes albopictus ranging from 8%-47% and 37%-78% respectively, indicating that this site could be a high-risk area for dengue outbreak. ae. aeg ...201121602768
does native bromeliads represent important breeding sites for aedes aegypti (l.) (diptera: culicidae) in urbanized areas?this study evaluates the importance of native bromeliads growing on rocky outcrops interspersed with urbanized areas as breeding sites for the aedes aegypti (l.) in vitória, state of espírito santo, brazil. oviposition traps were installed in backyards of houses in two separate zones. in the first zone houses were up to 50 m away from the rocky outcrops, while in the second zone they were at least at 200 m from the rocky outcrops. aedes aegypti was significantly more abundant in the latter zone. ...201121584412
breeding sites of aedes aegypti in metropolitan vacant lots in greater vitória, state of espírito santo, brazil.this study aimed to evaluate the presence of aedes aegypti in breeding sites located in vacant lots (vls) and determine the effectiveness of vl cleaning to reduce insect foci.201121552743
effects of different temperature regimens on the development of aedes aegypti (l.) (diptera: culicidae) mosquitoes.this study was conducted to determine the effects of increased water temperatures on the development of aedes aegypti immatures under laboratory conditions in trinidad, west indies using temperature regulated water baths to cover a range of temperatures from 24-25°c to 34-35°c at a relative humidity of 80%. two experiments were designed: (1) at constant temperature regimens and (2) under diurnal temperature regimens ranging from 24-25°c to 34-35°c. at 24-25°c egg hatching success was 98% at 48 h ...201121549680
[targeted treatment of aedes aegypti at localities with high risk for dengue transmission, morelos, mexico].to determine the relative importance of aedes aegyti breeding sites for potential targeted dengue control interventions in morelos.201321537805
aedes aegypti and aedes albopictus (diptera: culicidae): coexistence and susceptibility to temephos, in municipalities with occurrence of dengue and differentiated characteristics of urbanization.the aim of the present study was to verify the coexistence between aedes aegypti and aedes albopictus populations in municipalities of the states of paraná and santa catarina with different urbanization profiles where dengue occurs and evaluate their susceptibility to the organophosphate temephos.201321537795
[contributions made by the cuban advisory work to the entomological surveillance in haiti].the earthquake occurred in haiti in january 2010 led to the worsening of the hygienic, sanitary and environmental conditions and increased the number of breeding sites for disease-borne vectors in the country.201323437528
[effectiveness of insecticidal formulations for the aedes aegypti adult population control in la habana, cuba].the vector control remains the sole sustainable measure to prevent dengue transmission in which the key role goes to adulticides in case of high rates of infestation by the vector, outbreaks or dengue epidemics. pyrethroids are the most used whereas organophosphorous chloripirifos has been occasionally used because of the cost.201323437526
spatial modeling of dengue and socio-environmental indicators in the city of rio de janeiro, brazil.this study analyzed the spatial distribution of dengue in rio de janeiro, brazil, in 2006, and associations between the incidence per 100,000 inhabitants and socio-environmental variables. the study analyzed reported dengue cases among the city's inhabitants, rainfall, breteau index (for aedes aegypti and aedes albopictus), gini index, and social development index. we conducted mapping and used the global moran index to measure the indicators' spatial autocorrelation, which was positive for all ...201121519709
temephos resistance and esterase activity in the mosquito aedes aegypti in havana, cuba increased dramatically between 2006 and 2008.aedes aegypti (l.) (diptera: culicidae) control programmes in cuba rely on the application of the organophosphate temephos for larval control. hence, the monitoring of resistance to this insecticide is an essential component of such programmes. here, 15 field populations from different municipalities of havana city were assayed for resistance to temephos. high levels of resistance were detected in all strains and resistance ratios were highly correlated with esterase activity (p = 0.00001). popu ...201121501201
larvicidal activity of myrtaceae essential oils and their components against aedes aegypti, acute toxicity on daphnia magna, and aqueous residue.the larvicidal activity of 11 myrtaceae essential oils and their constituents was evaluated against aedes aegypti l. of the 11, melaleuca linariifolia sm., melaleuca dissitiflora f. muell., melaleuca quinquenervia (cav.) s. t. blake, and eucalyptus globulus labill oils at 0.1 mg/ml exhibited > or = 80% larval mortality. at this same concentration, the individual constituents tested, allyl isothiocyanate, alpha-terpinene, p-cymene, (+)-limonene, (-)-limonene, gamma-terpinene, and (e)-nerolidol, r ...201121485381
the mosquitocidal activity of methanolic extracts of lantana cramera root and anacardium occidentale leaf: role of glutathione s-transferase in insecticide resistance.larvicidal activity of methanolic plant extracts of lantana cramera (p1) root and anacardium occidentale (p2) leaf was investigated against the larvae of the three mosquito species (culex quinquefasciatus, anopheles stephensi, and aedes aegypti reared in the laboratory), and the respective glutathione s-transferase (gst) activity was analyzed as an index of protection against the extracts. the lc50 (extract concentration that shows 50% mortality) values of p1 extract for an. stephensi, ae. aegyp ...201121485364
variation in cold hardiness of nondiapausing eggs of nine aedes (stegomyia) species (diptera: culicidae) from eastern asia and pacific islands ranging from the tropics to the cool-temperate zone.variation in cold hardiness was studied in the laboratory for the nondiapausing eggs of nine aedes (stegomyia) species from eastern asia and pacific islands, ranging from the tropics to the cool-temperate zone. species included were aedes aegypti (l.), aedes albopictus (skuse), aedes flavopictus yamada, aedes galloisi yamada, aedes hensilli farner, aedes palauensis bohart, aedes paullusi stone and farner, aedes riversi bohart and ingram, and aedes scutellaris (walker). for species with distribut ...201121485356
mosquitocidal properties of nereistoxin against anopheles stephensi, aedes aegypti and culex quinquefasciatus (diptera: culicidae).emergence of resistance among mosquitoes is a recent problem. safe and eco-friendly agents from biological origins are the need of the hour. nereistoxin, a naturally occurring substance, was first isolated from the marine annelid lumbriconereis heteropoda and stored in the freezer. in the present study, the toxicity of nereistoxin was evaluated against vector mosquitoes. the larvicidal, ovicidal and adulticidal activities of nereistoxin were assayed for their toxicity against three important vec ...201121472401
[ultrastructural alterations in larvae of aedes aegypti subject to labdane diterpene isolated from copaifera reticulata (leguminosae) and a fraction enriched with tannins of magonia pubescens (sapindaceae)].dengue is an important public health problem in many countries and its main vector aedes aegypti, is the mosquito most adapted to urban areas. for the first time, the mechanism of action of labdane diterpenoid extracted from copaifera reticulata and the fraction enriched of catechin tannins extracted from magonia pubescens is demonstrated through ultrastructural alterations of aedes aegypti larvae.201621468474
evidence on impact of community-based environmental management on dengue transmission in santiago de cuba.during the dengue outbreak that struck santiago de cuba in 2006-2007, we conducted an observational study in the mariana grajales district, the former setting of a community trial for aedes aegypti control. in the trial, community working groups (cwg) had been created in 29 randomly selected intervention house blocks, and routine vector control activities alone were conducted in the remaining 30 control blocks. the cwg elaborated and implemented with the population plans and activities to reduce ...201121418448
larvicidal activity of major essential oils from stems of allium monanthum maxim. against aedes aegypti l.the stems of allium monanthum were extracted, and the major essential oil composition and larvicidal effects were studied. the analyses were conducted by gas chromatography and mass spectroscopy revealed that the essential oils of a. monanthum stems. the a. monanthum essential oil yield was 4.25%, and gas chromatography and mass spectroscopy analysis revealed that its major constituents were dimethyl trisulfide (23.21%), dimethyl tetrasulfide (11.24%) and methlyl propyl trisulfide (8.21%). the e ...201121417962
vertical dispersal of aedes (stegomyia) spp. in high-rise apartments in putrajaya, malaysia.a preliminary study on the vertical dispersal of aedes populations in high-rise apartments was carried out in presint 9, putrajaya, malaysia. ovitraps were placed indoors within four blocks of high-rise apartments from the ground floors (0.0 - 3.0 m) until up to the tenth floors (28.1 - 30.0 m). aedes aegypti was the dominant species found in the ovitraps (87.85%), while aedes albopictus was found in lower numbers. from total number of larvae collected (650), 40.92% of these larvae were obtained ...201021399609
larvicidal activities of six plants extracts against two mosquito species, aedes aegypti and anopheles stephensi.larvicidal activity of crude chloroform, dichloromethane and methanol extracts of the leaves and roots of six indian plants, aegle marmelos l., balanites aegyptica l., calotropis gigantica l., murraya koenigii l., nyctanthes arbor-tristis l. and plumbago zeylanica l., were tested against the early fourth instar larvae of aedes aegypti l. and anopheles stephensi. the larval mortality was observed after 24 h of exposure. all extracts showed moderate larvicidal effects. however, the highest larval ...201021399575
multiple activities of insect repellents on odorant receptors in mosquitoes.several lines of evidence suggest that insect repellent molecules reduce mosquito-host contacts by interacting with odorants and odorant receptors (ors), thereby ultimately affecting olfactory-driven behaviours. we describe the molecular effects of 10 insect repellents and a pyrethroid insecticide with known repellent activity on two highly specific aedes aegypti (diptera: culicidae) ors, aaor2 + aaor7 and aaor8 + aaor7, exquisitely sensitive to key mosquito attractants indole and (r)-(-)-1-octe ...201121395633
insecticide resistance status of aedes aegypti (l.) from colombia.to evaluate the insecticide susceptibility status of aedes aegypti (l.) in colombia, and as part of the national network of insecticide resistance surveillance, 12 mosquito populations were assessed for resistance to pyrethroids, organophosphates and ddt. bioassays were performed using who and cdc methodologies. the underlying resistance mechanisms were investigated through biochemical assays and rt-pcr.201121394876
structure-activity relationships of larvicidal monoterpenes and derivatives against aedes aegypti linn.in the search for larvicidal compounds against aedes aegypti l. (diptera: culicidae), a collection of monoterpenes were selected and evaluated. r- and s-limonene exhibited the highest larvicidal potency (lc(50)=27 and 30 ppm, respectively), followed by γ-terpinene (lc(50)=56 ppm) and rs-carvone (lc(50)=118 ppm). structural characteristics which may contribute to the understanding of the larvicidal activity of monoterpenes were empirically identified. the presence of heteroatoms in the basic hydr ...201121376365
high-throughput assays for detection of the f1534c mutation in the voltage-gated sodium channel gene in permethrin-resistant aedes aegypti and the distribution of this mutation throughout thailand.to develop rapid monitoring tools to detect the f1534c permethrin-resistance mutation in domain iiis6 of the aedes aegypti voltage-gated sodium channel gene and determine the frequency and distribution of this mutation in thailand.201121342372
enhanced repellency of binary mixtures of zanthoxylum armatum seed oil, vanillin, and their aerosols to mosquitoes under laboratory and field conditions.the repellency of zanthoxylum armatum seed oil (za-so), alone or in combination with vanillin (va), its six major constituents, and another four major previously known zanthoxylum piperitum fruit oil constituents, as well as aerosol products containing 5 or 10% za-so and 5% va, was evaluated against female aedes aegypti in laboratory and field studies. results were then compared with those of n,n-diethyl-3-methylbenzamide (deet) as a standard. hand in cage laboratory tests showed that 0.2, 0.1, ...201121337949
enzymes-based resistant mechanism in pyrethroid resistant and susceptible aedes aegypti strains from northern thailand.previous studies have shown that permethrin resistance in our selected pmd-r strain of aedes aegypti from chiang mai, thailand, was associated with a homozygous mutation in the knockdown resistance (kdr) gene and other mechanisms. in this study, we investigated the metabolic mechanism of resistance of this strain compared to the pmd strain which is susceptible to permethrin. the permethrin susceptibility of larvae was determined by a dose-response bioassay. two synergists, namely piperonyl butox ...201121336645
can piperonyl butoxide enhance the efficacy of pyrethroids against pyrethroid-resistant aedes aegypti?pyrethroid resistance can be considered the main threat to the continued control of many mosquito vectors of disease. piperonyl butoxide (pbo) has been used as a synergist to help increase the efficacy of certain insecticides. this enhancement stems from its ability to inhibit two major metabolic enzyme systems, p450s and non-specific esterases, and to enhance cuticular penetration of the insecticide.201121324051
expression of trypsin-like serine peptidases in pre-imaginal stages of aedes aegypti (diptera: culicidae).this study reports the biochemical characterization and comparative analyses of highly active serine proteases in the larval and pupal developmental stages of aedes aegypti (linnaeus) using substrate-sds-page. zymographic analysis of larval stadia detected proteolytic activity in 6-8 bands with apparent molecular masses ranging from 20 to 250 kda, with activity observed from ph 5.5 to 10.0. the pupal stage showed a complex proteolytic activity in at least 11 bands with apparent mr ranging from 2 ...201121308760
insecticide resistance status of aedes aegypti in 10 localities in colombia.insecticide resistance is one of the major threats to the effectiveness of vector control programs. in order to establish a baseline susceptibility profile of aedes aegypti in the southwest of colombia, 10 localities in four departments (states) were evaluated. standardized who bioassay, cdc bottle bioassay and microplate biochemical assays of non-specific β-esterase (nse), mixed function oxidases (mfo) and acetylcholinesterase were used. cross resistance was evaluated with field collected mosqu ...201121300017
chemical constituents and larvicidal activity of hymenaea courbaril fruit peel.the chemical compositions of the essential oils from the peel of ripe and unripe fruits of hymenaea courbaril l., obtained by hydrodistillation, were analyzed by gc and gc-ms. the main constituents of the essential oil from the peel of the ripe fruits were the sesquiterpenes alpha-copaene (11.1%), spathulenol (10.1%) and beta-selinene (8.2%), while germacrene-d (31.9%), beta-caryophyllene (27.1%) and bicyclogermacrene (6.5%) were the major compounds in the oil from unripe fruits. the essential o ...201021299135
characterisation of ddt and pyrethroid resistance in trinidad and tobago populations of aedes aegypti.insecticide resistance is an important factor in the effectiveness of aedes aegypti control and the related spread of dengue. the objectives of this study were to investigate the status of the organochlorine dichlorodiphenyltrichloroethane (ddt) and pyrethroid (permethrin and deltamethrin) resistance in trinidad and tobago populations of ae. aegypti and the underlying biochemical mechanisms. nine populations of ae. aegypti larvae from trinidad and tobago were assayed to ddt and pys using the cen ...201121272394
[the effect of water quality in the life cycle and in the attraction for the egg oviposition of aedes aegypti (l.) (diptera: culicidae)].the present research aimed at evaluating the influence of the water quality in the life cycle and attraction of aedes aegypti (l.) females to oviposit using different sources of water (raw sewage, effluent of uasb reactor, effluent of polishing lagoon, effluent of anaerobic filter, rain water and de-chlorinated water). the immature development time and survivorship were evaluated on a daily basis in two distinct feeding systems (with and without food). the quality of the water was shown to affec ...201321271073
the biological effects of the insect growth regulators; pyriproxyfen and diflubenzuron on the mosquito aedes aegypti.the biological effects of two insect growth regulators (igrs), pyriproxyfen and diflubenzuron against larvae of the mosquito aedes aegypti (l.) have been evaluated. mosquitoes were collected from jeddah governorate, west of the kingdom of saudi arabia and maintained in the laboratory. according to ic50 values obtained (concentrations which inhibit the emergence of 50% of adults), diflubenzuron (0.00036 ppm) proved to be more effective by 11.4 fold of pyriproxyfen (0.0041 ppm). in addition to the ...201021268527
isolation, primary culture and morphological characterization of oenocytes from aedes aegypti pupae.oenocytes are ectodermic cells that participate in a number of critical physiological roles such as detoxification and lipid storage and metabolism in insects. in light of the lack of information on oenocytes from aedes aegypti and the potential role of these cells in the biology of this major yellow fever and dengue vector, we developed a protocol to purify and maintain ae. aegypti pupa oenocytes in primary culture. ae. aegypti oenocytes were cultured as clustered and as isolated ovoid cells wi ...201121255811
distribution of aedes albopictus (diptera: culicidae) in northwestern china.the asian tiger mosquito, aedes albopictus (diptera: culicidae), has a wide distribution in china with its northwestern limits among seven provinces. during 2006-2008, distribution information was collected in 33 urban and rural areas in those boundary provinces by collecting larvae or adult mosquito from different breeding sites. additional information of seven sites was gathered from local health authorities. three generally accepted climatic delineations affecting distribution of the species ...201121254912
effects of extract of soapnut sapindus emarginatus on esterases and phosphatases of the vector mosquito, aedes aegypti (diptera: culicidae).our earlier investigations with kernels from the soapnut sapindus emarginatus revealed it as a new source of botanical biocide with potent antimosquito activity, as evident from the proven unique ability of the aqueous kernel extract to kill all the developmental stages of three important vector mosquito species, aedes aegypti, anopheles stephensi and culex quinquefasciatus. this extract was also found to be safe for two non-target aquatic insects. as a sequel to these findings, we have further ...201121251906
analysis of the workers head transcriptome of the asian subterranean termite, coptotermes gestroi.the lower termite, coptotermes gestroi (isoptera: rhinotermitidae), is originally from southeast asia and has become a pest in brazil. the main goal of this study was to survey c. gestroi transcriptome composition. to accomplish this, we sequenced and analyzed 3003 expressed sequence tags (ests) isolated from libraries of worker heads. after assembly, 695 uniests were obtained from which 349 have similarity with known sequences. comparison with insect genomes demonstrated similarity, primarily w ...201121205397
travel-related mosquito-transmitted disease questionnaire survey among health professionals in taiwan.health-care professionals can help travelers by providing accurate pre-travel counseling for mosquito-transmitted diseases such as malaria, yellow fever, and dengue fever. governments and international organizations will benefit from knowledge survey among health professionals in this field to promote the development of travel health profession. this study investigates physicians' and nurses' knowledge regarding malaria, yellow fever, and dengue fever.201521199140
the role of the aedes aegypti epsilon glutathione transferases in conferring resistance to ddt and pyrethroid insecticides.the epsilon glutathione transferase (gst) class in the dengue vector aedes aegypti consists of eight sequentially arranged genes spanning 53,645 bp on super contig 1.291, which maps to chromosome 2. one epsilon gst, gste2, has previously been implicated in conferring resistance to ddt. the amino acid sequence of gste2 in an insecticide susceptible and a ddt resistant strain differs at five residues two of which occur in the putative ddt binding site. characterization of the respective recombinan ...201121195177
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