| metabolite identification of the antimalarial naphthoquine using liquid chromatography-tandem high-resolution mass spectrometry in combination with multiple data-mining tools. | naphthoquine (nq) is one of important partner drugs of artemisinin-based combination therapy (act), which is recommended for the treatment of uncomplicated plasmodium falciparum. nq shows a high cure rate after a single oral administration. it is absorbed quickly (time to peak concentration 2-4 h) and has a long elimination half-life (255 h). however, the metabolism of nq has not been clarified. in this work, the metabolite profiling of nq was studied in six liver microsomal incubates (human, cy ... | 2018 | 29430676 |
| a cross-species comparison of abnormal behavior in three species of singly-housed old world monkeys. | abnormal behavior occurs in a number of captive nonhuman primate species and is often used as an indicator of welfare. however, reported levels of abnormal behavior often vary across species, making general welfare judgments difficult. the purpose of this study was to assess differences in levels of abnormal behavior and associated risk factors across three species of old world monkeys in order to identify similarities and differences across species. the subjects were 415 (109 females) cynomolgu ... | 2018 | 29422700 |
| inotropic effects of nicorandil on cardiac contractility assessed by left ventricular pressure-volume relationship analyses in anesthetized monkeys. | nicorandil is a representative antianginal drug that has dual properties of a nitrate and adenosine triphosphate-sensitive potassium (katp) channel agonist; however, its effects on integrated cardiac function have not been fully understood. this study was conducted to clarify the functional, hemodynamic, and electrophysiological effects of nicorandil using ventricular pressure-volume loop analysis in isoflurane-anesthetized monkeys. nicorandil was given intravenously at therapeutic doses of 0.2 ... | 2018 | 29420355 |
| rna-seq analysis of the transcriptome of the liver of cynomolgus monkeys with type 2 diabetes. | genetic and environmental factors such as high-fat diet are involved in the development of type 2 diabetes mellitus (t2dm). cynomolgus monkey shares similar genetic makeup, tissue structures, physiology and metabolic function to human. this study aimed to establish t2dm model in cynomolgus monkey and compare expression profiles of hepatic genes and their associated pathways in normal cynomolgus monkeys and those with t2dm. we employed rna-seq technique and identified 1451 differentially expresse ... | 2018 | 29414690 |
| discovery of a cynomolgus monkey family with retinitis pigmentosa. | to accelerate the development of new therapies, an inherited retinal degeneration model in a nonhuman primate would be useful to confirm the efficacy in preclinical studies. in this study, we describe the discovery of retinitis pigmentosa in a cynomolgus monkey (macaca fascicularis) pedigree. | 2018 | 29411010 |
| in vivo characterization of two 18f-labeled pde10a pet radioligands in nonhuman primate brains. | positron emission tomography (pet) with phosphodiesterase 10a (pde10a) specific radioligands provides a noninvasive and quantitative imaging tool to access the expression of this enzyme in vivo under normal and diseased conditions. we recently reported two potent 18f-labeled pde10a radioligands (18f-tz19106b and 18f-tz8110); initial evaluation in rats and nonhuman primates indicated stable metabolic profiles and excellent target-to-nontarget ratio (striatum/cerebellum) for both tracers. herein, ... | 2018 | 29400443 |
| a case report of spontaneous staphylococcal meningitis in a cynomolgus monkey. | this report describes a suppurative meningitis in a young cynomolgus. the animal had neutrophil aggregation in the subarachnoid space and hemorrhage in bilateral adrenal glands. staphylococcus was identified by fish in brain. to our knowledge, this is the first case of staphylococcal meningitis with waterhouse-friderichsen syndrome in a cynomolgus monkey. | 2018 | 29399828 |
| cloning of macaque monkeys by somatic cell nuclear transfer. | generation of genetically uniform non-human primates may help to establish animal models for primate biology and biomedical research. in this study, we have successfully cloned cynomolgus monkeys (macaca fascicularis) by somatic cell nuclear transfer (scnt). we found that injection of h3k9me3 demethylase kdm4d mrna and treatment with histone deacetylase inhibitor trichostatin a at one-cell stage following scnt greatly improved blastocyst development and pregnancy rate of transplanted scnt embryo ... | 2018 | 29395327 |
| analysis of carboxylesterase 2 transcript variants in cynomolgus macaque liver. | carboxylesterase (ces) is important for the detoxification of a wide range of drugs and xenobiotics. in this study, the hepatic level of ces2 mrna was examined in cynomolgus macaques used widely in preclinical studies for drug metabolism. three ces2 mrnas were present in cynomolgus macaque liver. the mrna level was highest for cynomolgus ces2a (formerly ces2v3), much lower for cynomolgus ces2b (formerly ces2v1) and extremely low for cynomolgus ces2c (formerly ces2v2). most various transcript var ... | 2019 | 29384423 |
| metamaterial-based transmit and receive system for whole-body magnetic resonance imaging at ultra-high magnetic fields. | magnetic resonance imaging (mri) at ultra-high fields (uhf), such as 7 t, provides an enhanced signal-to-noise ratio and has led to unprecedented high-resolution anatomic images and brain activation maps. although a variety of radio frequency (rf) coil architectures have been developed for imaging at uhf conditions, they usually are specialized for small volumes of interests (voi). so far, whole-body coil resonators are not available for commercial uhf human whole-body mri systems. the goal of t ... | 2018 | 29370245 |
| simultaneous quantification of endogenous and exogenous plasma glucose by isotope dilution lc-ms/ms with indirect mrm of the derivative tag. | quantification of endogenous and exogenous plasma glucose can help more comprehensively evaluate the glucose metabolic status. a ratio-based approach using isotope dilution liquid chromatography tandem mass spectrometry (id lc-ms/ms) with indirect multiple reaction monitoring (mrm) of the derivative tag was developed to simultaneously quantify endo-/exogenous plasma glucose. using diluted d-[13c6] glucose as tracer of exogenous glucose, 12c6/13c6 glucoses were first derivatized and then data wer ... | 2018 | 29362854 |
| curcumin as an in vivo selective intestinal breast cancer resistance protein inhibitor in cynomolgus monkeys. | to estimate the clinical impact of pharmacokinetic modulation via breast cancer resistance protein (bcrp), in vivo approaches in nonclinical settings are desired in drug development. clinical observation has identified curcumin as a promising candidate for in vivo selective bcrp inhibition, in addition to several well known inhibitors, such as lapatinib and pantoprazole. this study aimed to confirm the inhibitory efficacy of curcumin on gastrointestinal bcrp function in cynomolgus monkeys and to ... | 2018 | 29358184 |
| biotherapeutics in non-clinical development: strengthening the interface between safety, pharmacokinetics-pharmacodynamics and manufacturing. | biological drugs comprise a wide field of different modalities with respect to structure, pharmacokinetics and pharmacological function. considerable non-clinical experience in the development of proteins (e.g. insulin) and antibodies has been accumulated over the past thirty years. in order to improve the efficacy and the safety of these biotherapeutics, fc modifications (e.g. fc silent antibody versions), combinations (antibody-drug conjugates, protein-nanoparticle combinations), and new const ... | 2018 | 29355662 |
| in vitro model for predicting bioavailability of subcutaneously injected monoclonal antibodies. | monoclonal antibodies (mabs), which are now more frequently administered by subcutaneous (sc) injection rather than intravenously, have become a tremendously successful drug format across a wide range of therapeutic areas. preclinical evaluations of mabs to be administered by sc injection are typically performed in species such as mice, rats, minipigs, and cynomolgus monkeys to obtain critical information regarding formulation performance and prediction of pk/pd outcomes needed to select clinica ... | 2018 | 29355621 |
| glutazumab, a novel long-lasting glp-1/anti-glp-1r antibody fusion protein, exerts anti-diabetic effects through targeting dual receptor binding sites. | glucagon like-peptide-1 (glp-1)-based drugs have been proposed as mono- or combined therapy for type 2 diabetes mellitus. thus we characterized a novel antibody fusion protein engineered by linking the human glp-1 derivative to a humanized glp-1 receptor (glp-1r) antibody via a peptide linker. | 2018 | 29355505 |
| disrupting cd147-rap2 interaction abrogates erythrocyte invasion by plasmodium falciparum. | effective vaccines against malaria caused by plasmodium falciparum are still lacking, and the molecular mechanism of the host-parasite interaction is not fully understood. here we demonstrate that the interaction of rap2, a parasite-secreted rhoptry protein that functions in the parasitophorous vacuole formation stage of the invasion, and cd147 on the host erythrocyte is essential for erythrocyte invasion by p falciparum and is independent from all previously identified interactions involved. im ... | 2018 | 29352039 |
| lack of remuscularization following transplantation of human embryonic stem cell-derived cardiovascular progenitor cells in infarcted nonhuman primates. | human pluripotent stem cell-derived cardiovascular progenitor cells (hpsc-cvpcs) should be thoroughly investigated in large animal studies before testing in clinical trials. | 2018 | 29343525 |
| metabolism and pharmacokinetics of a potent n-acylindole antagonist of the oxe receptor for the eosinophil chemoattractant 5-oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ete) in rats and monkeys. | we previously identified the indole 264 as a potent in vitro antagonist of the human oxe receptor that mediates the actions of the powerful eosinophil chemoattractant 5-oxo-ete. no antagonists of this receptor are currently commercially available or are being tested in clinical studies. the lack of a rodent ortholog of the oxe receptor has hampered progress in this area because of the unavailability of commonly used mouse or rat animal models. in the present study, we examined the feasibility of ... | 2018 | 29339225 |
| technique for orthotopic liver transplantation in cynomolgus monkeys (macaca fascicularis). | recent studies investigating new strategies to modulate the immune system have utilized animal models of liver transplantation (lt). however, the anhepatic phase (ahp) remains a crucial problem in lt. the aim of the present study is to introduce a technique for successful orthotopic lt in cynomolgus monkeys using an early-reperfusion strategy. | 2018 | 29333420 |
| non-destructive determination of muscle architectural variables through the use of dicect. | the fascicular architecture of skeletal muscle dictates functional parameters such as force production and contractile velocity. muscle microarchitecture is typically determined by means of manual dissection, a technique that is inherently destructive to specimens. furthermore, fascicle lengths and pennation angles are commonly assessed at only a limited number of sampling sites per muscle. we present the results of a digital technique to non-destructively assess muscle architectural variables f ... | 2018 | 29330959 |
| sustained plasma hepcidin suppression and iron elevation by anticalin-derived hepcidin antagonist in cynomolgus monkey. | anaemia of chronic disease (acd) has been linked to iron-restricted erythropoiesis imposed by high circulating levels of hepcidin, a 25 amino acid hepatocyte-derived peptide that controls systemic iron homeostasis. here, we report the engineering of the human lipocalin-derived, small protein-based anticalin prs-080 hepcidin antagonist with high affinity and selectivity. | 2018 | 29329501 |
| generation of a precise oct4-hrgfp knockin cynomolgus monkey model via crispr/cas9-assisted homologous recombination. | | 2018 | 29327727 |
| generation of knock-in cynomolgus monkey via crispr/cas9 editing. | | 2018 | 29327726 |
| brain structural changes in cynomolgus monkeys administered with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine: a longitudinal voxel-based morphometry and diffusion tensor imaging study. | in animal models of parkinson's disease (pd), 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (mptp) is one of the most widely used agents that damages the nigrostriatal dopaminergic pathway. however, brain structural changes in response to mptp remain unclear. this study aimed to investigate in vivo longitudinal changes in gray matter (gm) volume and white matter (wm) microstructure in primate models administered with mptp. in six cynomolgus monkeys, high-resolution magnetic resonance imaging (mri ... | 2018 | 29320500 |
| selective cd28 inhibition modulates alloimmunity and cardiac allograft vasculopathy in anti-cd154-treated monkeys. | selective cd28 inhibition is actively pursued as an alternative to b7 blockade using cytotoxic t lymphocyte antigen 4 ig based on the hypothesis that the checkpoint immune regulators cytotoxic t lymphocyte antigen 4 and programmed death ligand 1 will induce tolerogenic immune signals. we previously showed that blocking cd28 using a monovalent nonactivating reagent (single-chain anti-cd28 fv fragment linked to alpha-1 antitrypsin [sc28at]) synergizes with calcineurin inhibitors in nonhuman primat ... | 2018 | 29319621 |
| usefulness of simultaneous and sequential monitoring of glucose level and electrocardiogram in monkeys treated with gatifloxacin under conscious and nonrestricted conditions. | drug-induced cardiac electrophysiological abnormalities accompanied by hypoglycemia or hyperglycemia increase the risk for life-threatening arrhythmia. to assess the drug-induced cardiotoxic potential associated with extraordinary blood glucose (glu) levels, the effect of gatifloxacin (gflx) which was frequently associated with glu abnormality and qt/qtc prolongations in the clinic on blood glu and electrocardiogram (ecg) parameters was investigated in cynomolgus monkeys (n=4) given gflx orally ... | 2018 | 29311442 |
| dual blockade of interleukin-1β and interleukin-17a reduces murine arthritis pathogenesis but also leads to spontaneous skin infections in nonhuman primates. | despite the efficacy of biologics for treatment of rheumatoid arthritis (ra), many patients show inadequate responses and likely require neutralization of multiple mediators. neutralization of both interleukin (il)-1β and il-17a with monoclonal antibodies showed greater efficacy than either agent alone in a mouse arthritis model with cooperative inhibition of key inflammatory factors, il-6, granulocyte colony-stimulating factor (g-csf), and cxc chemokine ligand (cxcl)1. given the potential clini ... | 2018 | 29311111 |
| proprioceptive changes in bilateral knee joints following unilateral anterior cruciate ligament injury in cynomolgus monkeys. | background the anterior cruciate ligament (acl) is one of the most important structures maintaining stability of knee joints, and the proprioception of the acl plays a key role in it. if the acl is injured in the unilateral knee joint, it changes nerve electrophysiology, morphology, and quantity of the proprioceptors in the bilateral acl. the aim of this study was to explore the proprioceptive changes in the bilateral knee joints following unilateral acl injury, and to provide a theoretical foun ... | 2018 | 29305572 |
| long-term nonhuman primate renal allograft survival without ongoing immunosuppression in recipients of delayed donor bone marrow transplantation. | we have previously reported successful induction of renal allograft tolerance in nonhuman primates (nhp) after an initial posttransplant period of conventional immunosuppression (delayed tolerance) using a nonmyeloablative conditioning regimen consisting of anti-cd154 and anti-cd8 mabs plus equine antithymocyte globulin (atgam) and donor bone marrow transplantation (dbmt). because these reagents are not currently clinically available, the protocol was revised to be applicable to human recipients ... | 2018 | 29300231 |
| adct-402, a pbd dimer-containing antibody drug conjugate targeting cd19-expressing malignancies. | human cd19 antigen is a 95-kda type i membrane glycoprotein in the immunoglobulin superfamily whose expression is limited to the various stages of b-cell development and differentiation and is maintained in the majority of b-cell malignancies, including leukemias and non-hodgkin lymphomas of b-cell origin. coupled with its differential and favorable expression profile, cd19 has rapid internalization kinetics and is not shed into the circulation, making it an ideal target for the development of a ... | 2018 | 29298756 |
| tlr2 agonism reverses chemotherapy-induced neutropenia in macaca fascicularis. | neutropenia is a common consequence of radiation and chemotherapy in cancer patients. the resulting immunocompromised patients become highly susceptible to potentially life-threatening infections. granulocyte colony-stimulating factor (g-csf) is known to stimulate neutrophil production and is widely used as a treatment of chemotherapy-induced neutropenia. a small-molecule g-csf secretagogue without a requirement for refrigerated supply chain would offer a more convenient and cost-effective treat ... | 2017 | 29296907 |
| retosiban prevents stretch-induced human myometrial contractility and delays labor in cynomolgus monkeys. | stretch of the myometrium promotes its contractility and is believed to contribute to the control of parturition at term and to the increased risk of preterm birth in multiple pregnancies. | 2018 | 29293998 |
| genome-wide mosaicism in divergence between zoonotic malaria parasite subpopulations with separate sympatric transmission cycles. | plasmodium knowlesi is a significant cause of human malaria transmitted as a zoonosis from macaque reservoir hosts in south-east asia. microsatellite genotyping has indicated that human infections in malaysian borneo are an admixture of two highly divergent sympatric parasite subpopulations that are, respectively, associated with long-tailed macaques (cluster 1) and pig-tailed macaques (cluster 2). whole-genome sequences of clinical isolates subsequently confirmed the separate clusters, although ... | 2018 | 29292549 |
| [effect of pushing manipulation on qiaogong acupoint on hemodynamics in cynomolgus monkeys with mild carotid atherosclerotic plaques]. | to explore the hemodynamic changes in cynomolgus monkeys with mild carotid atherosclerotic (cas) plaques after therapy with pushing manipulation on qiaogong acupoint (mpq). | 2017 | 29292250 |
| a novel quantification method for serine hydrolases in cellular expression system using fluorophosphonate-biotin probe. | in the present study, we established a quantitative western blotting method to measure the expression level of recombinant serine hydrolases based on their catalytic mechanism. fluorophosphonate (fp)-biotin was selected as a universal probe to quantify their expression levels, since fp moiety irreversibly inhibits serine hydrolases through strong stoichiometric binding to active serine residue. the linearity of detection using fp-biotin was assessed on three serine hydrolases; human carboxyleste ... | 2018 | 29289670 |
| rna expression profiling of nonhuman primate renal allograft rejection identifies tolerance. | tolerance induction to prevent allograft rejection is a long-standing clinical goal. however, convincing and dependable tolerance identification remains elusive. hypothesizing that intragraft rna expression is informative in both rejection and tolerance, we profile intrarenal allograft rna expression in a mixed chimerism renal allograft model of cynomolgus monkeys and identify biologically significant tolerance. analysis of 67 genes identified 3 dominant factors, each with a different pattern of ... | 2018 | 29288556 |
| the 17,18-epoxyeicosatetraenoic acid-g protein-coupled receptor 40 axis ameliorates contact hypersensitivity by inhibiting neutrophil mobility in mice and cynomolgus macaques. | metabolites of eicosapentaenoic acid exert various physiologic actions. 17,18-epoxyeicosatetraenoic acid (17,18-epete) is a recently identified new class of antiallergic and anti-inflammatory lipid metabolite of eicosapentaenoic acid, but its effects on skin inflammation and the underlying mechanisms remain to be investigated. | 2018 | 29288079 |
| rna expression profiling of renal allografts in a nonhuman primate identifies variation in nk and endothelial gene expression. | rna transcript expression estimates are a promising method to study the mechanisms and classification of renal allograft rejections. here we use the nanostring platform to profile rna expression in renal allografts in a nonhuman primate (nhp), the cynomolgus monkey. we analyzed protocol and indication 278 archival renal allograft samples, both protocol and indication from 76 animals with diagnoses of chronic antibody-mediated rejection (camr), acute cellular rejection (tcmr), and mixed (both cam ... | 2018 | 29286578 |
| an anti-gdnf family receptor alpha 1 (gfra1) antibody-drug conjugate for the treatment of hormone receptor-positive breast cancer. | luminal a (hormone receptor-positive) breast cancer constitutes 70% of total breast cancer patients. in an attempt to develop a targeted therapeutic for this cancer indication, we have identified and characterized glial cell line-derived neurotrophic factor (gdnf) family receptor alpha 1 (gfra1) antibody-drug conjugates (adc) using a cleavable valine-citrulline-mmae (vcmmae) linker-payload. rnaseq and ihc analysis confirmed the abundant expression of gfra1 in luminal a breast cancer tissues, whe ... | 2018 | 29282299 |
| melengestrol acetate (veterinary medicinal products). | food safety commission of japan (fscj) conducted a risk assessment of melengestrol acetate (mga, cas no. 2919-66-6), a synthetic hormone, based on results from various studies. mga was recognized to have no genotoxicity relevant to human health, and it enabled fscj to specify an acceptable daily intake (adi) in the assessment. in vitro studies using various human hormone-receptors showed that mga exerts biological action primarily as progestogens and secondarily as glucocorticoids. major adverse ... | 2017 | 32231940 |
| combinatorial inputs to the ventral striatum from the temporal cortex, frontal cortex, and amygdala: implications for segmenting the striatum. | the canonical striatal map, based predominantly on frontal corticostriatal projections, divides the striatum into ventromedial-limbic, central-association, and dorsolateral-motor territories. while this has been a useful heuristic, recent studies indicate that the striatum has a more complex topography when considering converging frontal and nonfrontal inputs from distributed cortical networks. the ventral striatum (vs) in particular is often ascribed a "limbic" role, but it receives diverse inf ... | 2018 | 29279863 |
| formulation and in vivo pharmacokinetic evaluation of ethyl cellulose-coated sustained release multiple-unit system of tacrolimus. | a novel once-a-day sustained-release (sr) system of tacrolimus (fk506), a poorly water-soluble immunosuppressive agent, was designed employing ethyl cellulose (ec) polymer as release retardant. drug (5 mg) was layered onto sugar spheres (518.3 mg) with hypromellose (5 mg), to transform the drug from a crystalline to an amorphous form. subsequently, the drug-layered pellets were recoated with ec polymer (0.5-1.5 mg) using a fluid bed granulator. drug release from the reservoir-type pellets was ma ... | 2018 | 29277422 |
| abaloparatide, a novel pth receptor agonist, increased bone mass and strength in ovariectomized cynomolgus monkeys by increasing bone formation without increasing bone resorption. | abaloparatide, a novel pth1 receptor agonist, increased bone formation in osteopenic ovariectomized cynomolgus monkeys while increasing cortical and trabecular bone mass. abaloparatide increased bone strength and maintained or enhanced bone mass-strength relationships, indicating preserved or improved bone quality. | 2018 | 29260289 |
| physiologic correlates of interactions between adult male and immature long-tailed macaques (macaca fascicularis). | interactions between adult males and immature members of the same species are rare in most mammals; in contrast, an estimated 40% of primate species are characterized by an involvement of males in the social life of infants and juveniles. the proximate mechanisms of male-infant interactions are largely unstudied, and very few direct benefits for males have been proposed, especially in uniparental species in which the identity of the male parent is uncertain. in this study, we aimed to assess the ... | 2017 | 29256366 |
| dihydrobenzisoxazole-4-one compounds are novel selective inhibitors of aldosterone synthase (cyp11b2) with in vivo activity. | 6,7-dihydro-5h-2,1-benzisoxazol-4-one analogs are potent inhibitors of aldosterone synthase (cyp11b2) with selectivity over the highly homologous enzyme cortisol synthase (cyp11b1). these compounds are unique among inhibitors of cyp11b2 in their lack of a strong-heme binding group such as a pyridine or imidazole. poor metabolic stability in hepatocyte incubations was found to proceed via a reduction of the isoxazole ring. while the enzyme responsible for the reductive metabolism remains unknown, ... | 2018 | 29254646 |
| müller glial cells of the primate foveola: an electron microscopical study. | previous studies on the ultrastructure of the primate foveola suggested the presence of an inverted cone-like structure which is formed by 25-35 specialized müller cells overlying the area of high photoreceptor density. we investigated the ultrastructure of the müller cells in the foveola of a human and macaque retina. sections through the posterior poles of an eye of a 40 years-old human donor and an eye of an adult cynomolgus monkey (macaca fascicularis) were investigated with transmission ele ... | 2018 | 29242027 |
| multivalent fcγ-receptor engagement by a hexameric fc-fusion protein triggers fcγ-receptor internalisation and modulation of fcγ-receptor functions. | engagement of fcγ-receptors triggers a range of downstream signalling events resulting in a diverse array of immune functions. as a result, blockade of fc-mediated function is an important strategy for the control of several autoimmune and inflammatory conditions. we have generated a hexameric-fc fusion protein (hexameric-fc) and tested the consequences of multi-valent fcγ-receptor engagement in in vitro and in vivo systems. in vitro engagement of hexameric-fc with fcγrs showed complex binding i ... | 2017 | 29213127 |
| novel d-galactosamine-induced cynomolgus monkey model of acute liver failure. | to establish a simplified, reproducible d-galactosamine-induced cynomolgus monkey model of acute liver failure having an appropriate treatment window. | 2017 | 29204057 |
| erratum: samhd1 polymorphisms were significantly correlated with hiv/siv virus load in pbmc from chinese rhesus macaques and cynomolgus macaques | | 2017 | 29197320 |
| whole body and cns biodistribution of rhhns in cynomolgus monkeys after intrathecal lumbar administration: treatment implications for patients with mps iiia. | mucopolysaccharidosis iii type a (mps iiia; sanfilippo syndrome), a genetic lysosomal disorder causing a deficiency of heparan n-sulfatase (hns), leads to progressive cognitive decline from an early age. an effective enzyme replacement therapy (ert) for mps iiia requires central nervous system (cns) biodistribution. recombinant human heparan n-sulfatase (rhhns), an investigatory ert for mps iiia, has been formulated for intrathecal (it) administration since intravenous (iv) administration cannot ... | 2017 | 29194406 |
| safety evaluation of lipid nanoparticle-formulated modified mrna in the sprague-dawley rat and cynomolgus monkey. | the pharmacology, pharmacokinetics, and safety of modified mrna formulated in lipid nanoparticles (lnps) were evaluated after repeat intravenous infusion to rats and monkeys. in both species, modified mrna encoding the protein for human erythropoietin (hepo) had predictable and consistent pharmacologic and toxicologic effects. pharmacokinetic analysis conducted following the first dose showed that measured hepo levels were maximal at 6 hours after the end of intravenous infusion and in excess of ... | 2018 | 29191134 |
| subretinal and intravitreal retinal injections in monkeys. | ocular anatomy and physiology in cynomolgus monkeys (macacca fascicularis) are very similar to that found in the human visual system. therefore and despite significant ethical and economical implications, these animals constitute an excellent model system for toxicology and biodistribution studies in the development of new and meaningful treatment strategies for ocular disorders. the methods for delivery of investigational new drugs (inds) to the ocular tissue are virtually identical to the meth ... | 2018 | 29188519 |
| first molecular characterization of balantioides coli (malmsten, 1857) isolates maintained in vitro culture and from feces of captive animals, rio de janeiro, brazil. | ciliate protozoa of the genus balantioides can parasitize a variety of animals. the morphology of the evolutionary forms of the parasite and the host species affected have long been the only characteristics used to taxonomically identify the species of these protozoa, but these variables are not very precise. to confirm species identity, molecular biology tools are currently used. in this context, this study aimed to analyze protozoan isolates maintained in culture medium and from fecal samples ... | 2017 | 31014580 |
| designing an immunocyte-targeting delivery system by use of beta-glucan. | a β-1,3-d-glucan called schizophyllan (spg) can form a novel complex with homo oligodeoxynucleotides (odns) via the combination of hydrogen bonding and hydrophobic interactions. dectin-1 is a major receptor involved in the recognition of β-1,3-d-glucans and expressed on antigen presenting cells (apcs) including macrophages, dendritic cells, monocytes, neutrophils, and a subset of t cells. therefore, the spg/odn complex can be used as apcs cell-specific delivery of functional odns including unmet ... | 2018 | 29174675 |
| the cellular composition and glia-neuron ratio in the spinal cord of a human and a nonhuman primate: comparison with other species and brain regions. | the cellular composition of brains shows largely conserved, gradual evolutionary trends between species. in the primate spinal cord, however, the glia-neuron ratio was reported to be greatly increased over that in the rodent spinal cord. here, we re-examined the cellular composition of the spinal cord of one human and one nonhuman primate species by employing two different counting methods, the isotropic fractionator and stereology. we also determined whether segmental differences in cellular co ... | 2018 | 29150977 |
| polymyxin derivatives nab739 and nab815 are more effective than polymyxin b in murine escherichia coli pyelonephritis. | extremely multiresistant strains of enterobacteriaceae, such as those of escherichia coli and klebsiella pneumoniae, are emerging and spreading at a worrisome speed. polymyxins (polymyxin b, colistin) are used as last-line therapy against such strains, in spite of their notable nephrotoxicity that may even require discontinuation of the therapy. we have previously developed polymyxin derivatives nab739 and nab815 that are better tolerated in cynomolgus monkeys than polymyxin b and are, in contra ... | 2018 | 29149329 |
| cat-02-106, a site-specifically conjugated anti-cd22 antibody bearing an mdr1-resistant maytansine payload yields excellent efficacy and safety in preclinical models. | hematologically derived tumors make up ∼10% of all newly diagnosed cancer cases in the united states. of these, the non-hodgkin lymphoma (nhl) designation describes a diverse group of cancers that collectively rank among the top 10 most commonly diagnosed cancers worldwide. although long-term survival trends are improving, there remains a significant unmet clinical need for treatments to help patients with relapsed or refractory disease, one cause of which is drug efflux through upregulation of ... | 2018 | 29142069 |
| a safety and immunogenicity study of a novel subunit plague vaccine in cynomolgus macaques. | plague has led to millions of deaths in history and outbreaks continue to the present day. the efficacy limitations and safety concerns of the existing killed whole cell and live-attenuated vaccines call for the development of new vaccines. in this study, we evaluated the immunogenicity and safety of a novel subunit plague vaccine, comprising native f1 antigen and recombinant v antigen. the cynomolgus macaques in low- and high-dose vaccine groups were vaccinated at weeks 0, 2, 4 and 6, at dose l ... | 2018 | 29134676 |
| allogeneic stem cell transplantation in fully mhc-matched mauritian cynomolgus macaques recapitulates diverse human clinical outcomes. | allogeneic hematopoietic stem cell transplantation (hsct) is a critically important therapy for hematological malignancies, inborn errors of metabolism, and immunodeficiency disorders, yet complications such as graft-vs.-host disease (gvhd) limit survival. development of anti-gvhd therapies that do not adversely affect susceptibility to infection or graft-vs.-tumor immunity are hampered by the lack of a physiologically relevant, preclinical model of allogeneic hsct. here we show a spectrum of di ... | 2017 | 29127275 |
| endodontic treatment of canine teeth in a cynomolgus monkey ( macaca fascicularis). | a case of bilateral superior canine tooth pulp necrosis in an 18-year-old male macaca fascicularis is described . a skull computed tomography scan was performed to confirm the diagnostic suspicion. a successful bilateral endodontic treatment with coronotomy was performed using rotary nickel-titanium instruments. post-operative dental radiographs confirmed the correct positioning of the sealant. a rapid and excellent clinical recovery was obtained after each surgery. this innovative approach may ... | 2018 | 29126360 |
| temporal cytokine and lymphoid responses to an inhaled tlr7 antedrug agonist in the cynomolgus monkey demonstrates potential safety and tolerability of this approach. | azd8848 is a tlr7 agonist antedrug developed for administration by inhalation dosing for the treatment of allergic diseases, such as asthma. allergic asthma is associated with increased levels of th2 cytokines which are suppressed for extended periods by tlr7 agonists in a number of preclinical models of allergic airway inflammation. however, tlrs form a central part of innate immunity and their activation often results in proinflammatory responses. whilst azd8848's antedrug mechanism is designe ... | 2018 | 29122671 |
| a mouse anti-myostatin antibody increases muscle mass and improves muscle strength and contractility in the mdx mouse model of duchenne muscular dystrophy and its humanized equivalent, domagrozumab (pf-06252616), increases muscle volume in cynomolgus monkeys. | the treatments currently approved for duchenne muscular dystrophy (dmd), a progressive skeletal muscle wasting disease, address the needs of only a small proportion of patients resulting in an urgent need for therapies that benefit all patients regardless of the underlying mutation. myostatin is a member of the transforming growth factor-β (tgf-β) family of ligands and is a negative regulator of skeletal muscle mass. loss of myostatin has been shown to increase muscle mass and improve muscle fun ... | 2017 | 29121992 |
| development and characterisation of a novel glucagon like peptide-1 receptor antibody. | glucagon like peptide-1 (glp-1) enhances glucose-dependent insulin secretion by binding to glp-1 receptors (glp1rs) on pancreatic beta cells. glp-1 mimetics are used in the clinic for the treatment of type 2 diabetes, but despite their therapeutic success, several clinical effects of glp-1 remain unexplained at a mechanistic level, particularly in extrapancreatic tissues. the aim of this study was to generate and characterise a monoclonal antagonistic antibody for the glp1r for use in vivo. | 2018 | 29119245 |
| host responses to concurrent combined injuries in non-human primates. | multi-organ failure (mof) following trauma remains a significant cause of morbidity and mortality related to a poorly understood abnormal inflammatory response. we characterized the inflammatory response in a non-human primate soft tissue injury and closed abdomen hemorrhage and sepsis model developed to assess realistic injury patterns and induce mof. | 2017 | 29118676 |
| neuroactive steroid (3α,5α)3-hydroxypregnan-20-one (3α,5α-thp) and pro-inflammatory cytokine mcp-1 levels in hippocampus ca1 are correlated with voluntary ethanol consumption in cynomolgus monkey. | neuroactive steroids such as (3α,5α)3-hydroxypregnan-20-one (3α,5α-thp, allopregnanolone) are potent neuromodulators that enhance gabaergic neurotransmission and produce inhibitory neurobehavioral and anti-inflammatory effects. chronic ethanol (etoh) consumption reduces 3α,5α-thp levels in human plasma, but has brain region- and species-specific effects on central nervous system levels of 3α,5α-thp. we explored the relationship between 3α,5α-thp levels in the hippocampus and voluntary etoh consu ... | 2018 | 29112774 |
| age-dependent elevations of oligomeric and phosphorylated alpha-synuclein synchronously occurs in the brain and gastrointestinal tract of cynomolgus monkeys. | fibrillary α-synuclein (α-syn), which constitutes the major component of lewy pathology characterized for parkinson's disease (pd), is found also in the aged enteric nervous system (ens) and central nervous system (cns). however, what happens to oligomeric α-syn (o-α-syn) in the aged ens and cns remains poorly understood. here, by using elisa methods specific for o-α-syn and phosphorylated α-syn (p-α-syn), we measured the levels of o-α-syn and p-α-syn in the gastrointestinal (gi) tract and brain ... | 2018 | 29107086 |
| genetic analysis of samples from wild populations opens new perspectives on hybridization between long-tailed (macaca fascicularis) and rhesus macaques (macaca mulatta). | in the past decade, many researchers have published papers about hybridization between long-tailed and rhesus macaques. these previous works have proposed unidirectional gene flow with the isthmus of kra as the zoogeographical barrier of hybridization. however, these reports analyzed specimens of unknown origin and/or did not include specimens from thailand, the center of the proposed area of hybridization. collected specimens of long-tailed and rhesus macaques representing all suspected hybridi ... | 2017 | 29095514 |
| the fcrn inhibitor rozanolixizumab reduces human serum igg concentration: a randomized phase 1 study. | pathogenic immunoglobulin g (igg) autoantibodies characterize some human autoimmune diseases; their high concentration and long half-life are dependent on recycling by the neonatal fc receptor (fcrn). inhibition of fcrn is an attractive new treatment concept for igg-mediated autoimmune diseases. rozanolixizumab (ucb7665; ca170_01519.g57 igg4p) is an anti-human fcrn monoclonal antibody. in cynomolgus monkeys, rozanolixizumab reduced igg (maximum 75 to 90% by about day 10), was well tolerated, and ... | 2017 | 29093180 |
| half-life extension using serum albumin-binding darpin® domains. | a long systemic half-life is key for therapeutic proteins. to that end we have generated serum albumin-binding designed ankyrin repeat domains. these domains bind serum albumin of different species with nanomolar affinities, and have significantly improved pharmacokinetic properties both in mouse and cynomolgus monkey compared to non-serum albumin-binding darpin® domains. in addition, they exhibit high thermal stability and long storage stability, which is an essential feature for their use in d ... | 2017 | 29088432 |
| extractive foraging and tool-aided behaviors in the wild nicobar long-tailed macaque (macaca fascicularis umbrosus). | macaques possess a repertoire of extractive foraging techniques that range from complex manipulation to tool-aided behaviors, to access food items that increase their foraging efficiency substantially. however, the complexity and composition of such techniques vary considerably between species and even between populations. in the present study, we report seven such complex manipulative behaviors that include six extractive foraging behaviors, and teeth flossing, in a population of nicobar long-t ... | 2018 | 29086889 |
| relationships of depressive behavior and sertraline treatment with walking speed and activity in older female nonhuman primates. | depression is the most common mental health problem in aging persons and is a leading risk factor for physical disability, especially in women. though antidepressant drugs such as serotonin reuptake inhibitors (ssri) are commonly prescribed, epidemiological evidence reveals mixed effects of long-term antidepressant use on physical function and activity, possibly depending on depressive status. the purpose of this preclinical trial was to determine the relationships of depressive behavior and the ... | 2017 | 29080976 |
| allowable warm ischemic time and morphological and biochemical changes in uterine ischemia/reperfusion injury in cynomolgus macaque: a basic study for uterus transplantation. | | 2017 | 29077872 |
| immunoaffinity lc-ms/ms for quantitative determination of a free and total protein target as a target engagement biomarker. | ip-10 is a protein target for the treatment of crohn's disease. inhibition of ip-10 by anti-ip-10 mabs neutralizes its various biological activities. the measurement of free ip-10 suppression as a target engagement biomarker is required for the assessment of drug effect on the target. | 2017 | 29072496 |
| transient changes in hepatic physiology that alter bilirubin and bile acid transport may explain elevations in liver chemistries observed in clinical trials of ggf2 (cimaglermin alfa). | ggf2 is a recombinant human neuregulin-1β in development for chronic heart failure. phase 1 clinical trials of ggf2 were put on hold when transient elevations in serum aminotransferases and total bilirubin were observed in 2 of 43 subjects who received single doses of ggf2 at 1.5 or 0.378 mg/kg. however, aminotransferase elevations were modest and not typical of liver injury sufficient to result in elevated serum bilirubin. cynomolgus monkeys administered a single 15 mg/kg dose of ggf2 had simil ... | 2018 | 29069498 |
| transabdominal ultrasound-guided multifetal pregnancy reduction in 10 cases of monkeys. | intracytoplasmic sperm injection (icsi) and embryo transfer (et) in nonhuman primates, e.g. rhesus and cynomolgus monkeys, has been widely used in researches of reproductive and developmental biology, and the success rate has been improved significantly. however, unwanted multiple pregnancy occurs frequently during the icsi-et in monkeys, most of which leads to miscarriages. to improve the birth rate of pregnancies and to safeguard health of host and baby monkeys, multifetal pregnancy reduction ... | 2017 | 29069285 |
| effects of carbon monoxide on early dysfunction and microangiopathy following galt-ko porcine pulmonary xenotransplantation in cynomolgus monkeys. | despite progress in the current genetic manipulation of donor pigs, most non-human primates were lost within a day of receiving porcine lung transplants. we previously reported that carbon monoxide (co) treatment improved pulmonary function in an allogeneic lung transplant (ltx) model using miniature swine. in this study, we evaluated whether the perioperative treatment with low-dose inhalation of co has beneficial effects on porcine lung xenografts in cynomolgus monkeys (cynos). | 2018 | 29067747 |
| technological response of wild macaques (macaca fascicularis) to anthropogenic change. | anthropogenic disturbances have a detrimental impact on the natural world; the vast expansion of palm oil monocultures is one of the most significant agricultural influences. primates worldwide consequently have been affected by the loss of their natural ecosystems. long-tailed macaques (macaca fascilularis) in southern thailand have, however, learned to exploit oil palm nuts using stone tools. using camera traps, we captured the stone tool behavior of one macaque group in ao phang-nga national ... | 2017 | 29056799 |
| effect of human milk formula with bovine colostrum supplementation on bone mineral density in infant cynomolgus macaques. | insulin-like growth factor 1 (igf1) is a regulator of human growth during infancy and childhood, known to promote bone and muscle growth as well as lipid accumulation. this study aimed to investigate the effects of formula milk with or without igf1 supplementation (in the form of pure igf1 or bovine colostrum) on growth and body composition in infant cynomolgus macaques during the first 6 months of life. three groups of infants were nursery-reared and received formula milk with or without igf1 o ... | 2018 | 29039296 |
| design and characterization of mp0250, a tri-specific anti-hgf/anti-vegf darpin® drug candidate. | mp0250 is a multi-domain drug candidate currently being tested in clinical trials for the treatment of cancer. it comprises one anti-vascular endothelial growth factor-a (vegf-a), one anti-hepatocyte growth factor (hgf), and two anti-human serum albumin (hsa) darpin® domains within a single polypeptide chain. while there is first clinical validation of a single-domain darpin® drug candidate, little is known about darpin® drug candidates comprising multiple domains. here, we show that mp0250 can ... | 2019 | 29035637 |
| synthesis and biologic evaluation of a novel 18f-labeled adnectin as a pet radioligand for imaging pd-l1 expression. | the programmed death protein (pd-1) and its ligand (pd-l1) play critical roles in a checkpoint pathway cancer cells exploit to evade the immune system. a same-day pet imaging agent for measuring pd-l1 status in primary and metastatic lesions could be important for optimizing drug therapy. herein, we have evaluated the tumor targeting of an anti-pd-l1 adnectin after 18f-fluorine labeling. methods: an anti-pd-l1 adnectin was labeled with 18f in 2 steps. this synthesis featured fluorination of a no ... | 2018 | 29025984 |
| riparian vegetation structure and the hunting behavior of adult estuarine crocodiles. | riparian ecosystems are amongst the most biodiverse tropical habitats. they are important, and essential, ecological corridors, linking remnant forest fragments. in this study, we hypothesised that crocodile's actively select nocturnal resting locations based on increased macaque predation potential. we examined the importance of riparian vegetation structure in the maintenance of crocodile hunting behaviours. using airborne light detection and ranging (lidar) and gps telemetry on animal movemen ... | 2017 | 29020111 |
| physiologically based pharmacokinetic modeling of transporter-mediated hepatic clearance and liver partitioning of oatp and oct substrates in cynomolgus monkeys. | in the present investigations, we evaluate in vitro hepatocyte uptake and partitioning for the prediction of in vivo clearance and liver partitioning. monkeys were intravenously co-dosed with rosuvastatin and bosentan, substrates of the organic anion transporting polypeptides (oatps), and metformin, a substrate of organic cation transporter 1 (oct1). serial plasma and liver samples were collected over time. liver and plasma unbound fraction was determined using equilibrium dialysis. in vivo unbo ... | 2017 | 29019117 |
| collagen sponge functionalized with chimeric anti-bmp-2 monoclonal antibody mediates repair of nonunion tibia defects in a nonhuman primate model: an exploratory study. | recombinant human bone morphogenetic protein (bmp)-2 is an fda-approved therapy for nonunion tibia fracture, though it has a number of biological and practical disadvantages. our research group has developed a novel tissue engineering strategy termed antibody-mediated osseous regeneration. this entails application of anti-bmp-2 monoclonal antibodies (mabs) to capture endogenous bmp's to mediate in vivo bone formation. this has been documented in a number of animal models. the present exploratory ... | 2017 | 28992803 |
| an anti-glypican 3/cd3 bispecific t cell-redirecting antibody for treatment of solid tumors. | cancer care is being revolutionized by immunotherapies such as immune checkpoint inhibitors, engineered t cell transfer, and cell vaccines. the bispecific t cell-redirecting antibody (trab) is one such promising immunotherapy, which can redirect t cells to tumor cells by engaging cd3 on a t cell and an antigen on a tumor cell. because t cells can be redirected to tumor cells regardless of the specificity of t cell receptors, trab is considered efficacious for less immunogenic tumors lacking enou ... | 2017 | 28978751 |
| healing of two-wall intra-bony defects treated with a novel emd-liquid-a pre-clinical study in monkeys. | to investigate the effect of a novel enamel matrix derivative formulation (emd-liquid or osteogain) combined with an absorbable collagen sponge (acs) on periodontal wound healing in intra-bony defects in monkeys. | 2017 | 28965367 |
| hazard characterization of an anti-human tissue factor antibody by combining results of tissue cross-reactivity studies and distribution of hemorrhagic lesions in monkey toxicity studies. | tissue cross-reactivity (tcr) studies are conducted when developing therapeutic antibodies, but their value is sometimes questioned because the positive organs often do not match the target organs of toxicity. we conducted tcr studies in human and cynomolgus monkey tissues for the development of an anti-human tissue factor antibody (tfab) and also for a commercially available antibody, to clarify the true distribution of the target antigen. tissue factor (tf) was found to be distributed in a wid ... | 2017 | 28964847 |
| chronic toxicity test in cynomolgus monkeys for 98 days with repeated intravenous infusion of cynomolgus umbilical cord mesenchymal stem cells. | stem cell-based therapy is attractive in many clinical studies, but current data on the safety of stem cell applications remains inadequate. this study observed the safety, immunological effect of cynomolgus monkey umbilical cord mesenchymal stem cells (muc-mscs) injected into cynomolgus monkeys, in order to evaluate the safety of human umbilical cord mesenchymal stem cells (huc-mscs) prepared for human clinical application. | 2017 | 28957810 |
| [effect of electroacupuncture on proprioception in cynomolgus monkeys after unilateral anterior cruciate ligament injury]. | to study the effect of electroacupuncture on proprioception in cynomolgus monkeys after unilateral anterior cruciate ligament (acl) injury. | 2017 | 28951357 |
| [11c]az10419096 - a full antagonist pet radioligand for imaging brain 5-ht1b receptors. | the serotonergic system is widely present in all regions of the central nervous system (cns) and plays a key modulatory role in many of its functions. positron emission tomography (pet) is used to study several serotonin receptors in cns in vivo. the g-protein coupled receptor 5-ht1b is mostly present in the occipital cortex and in midbrain and is linked to several psychiatric disorders. there is evidence that agonist pet radioligands for neuroreceptors are more sensitive to endogenous neurotran ... | 2017 | 28950161 |
| small-scale variability in a mosaic tropical rainforest influences habitat use of long-tailed macaques. | pristine habitats have generally been considered to be the most important ecological resource for wildlife conservation, but due to forest degradation caused by human activities, mosaics of secondary forests have become increasingly prominent. we studied three forest types in a mosaic tropical forest consisting of short secondary forest (ss), tall secondary forest (ts) and freshwater swamp forest (sf). these forests differed in stand structure and floristic composition, as well as phenological p ... | 2018 | 28948386 |
| rhesus macaques are more susceptible to progressive tuberculosis than cynomolgus macaques: a quantitative comparison. | in the past 2 decades, it has become increasingly clear that nonhuman primates, specifically macaques, are useful models for human tuberculosis (tb). several macaque species have been used for tb studies, and questions remain about the similarities and differences in tb pathogenesis among macaque species, which can complicate decisions about the best species for a specific experiment. here we provide a quantitative assessment, using serial positron emission tomography and computed tomography (pe ... | 2018 | 28947646 |
| the corticostriatal adenosine a2a receptor controls maintenance and retrieval of spatial working memory. | working memory (wm) taps into multiple executive processes including encoding, maintenance, and retrieval of information, but the molecular and circuit modulation of these wm processes remains undefined due to the lack of methods to control g protein-coupled receptor signaling with temporal resolution of seconds. | 2018 | 28941549 |
| novel h-shunt venovenous bypass for liver transplantation in cynomolgus macaques. | cynomolgus monkeys are often used in preclinical transplantation research. performing liver transplantation in cynomolgus monkeys is challenging because they poorly tolerate portal vein clamping during the anhepatic phase. finding an alternative to portal vein clamping is necessary before preclinical liver transplant models can be performed with reliable outcomes. we used 3 different techniques to perform 5 liver transplants in male cynomolgus macaques (weight, 7.4-10.8 kg; mismatched for mhc i ... | 2017 | 28935006 |
| analysis of 18fdg pet/ct imaging as a tool for studying mycobacterium tuberculosis infection and treatment in non-human primates. | mycobacterium tuberculosis remains the number one infectious agent in the world today. with the emergence of antibiotic resistant strains, new clinically relevant methods are needed that evaluate the disease process and screen for potential antibiotic and vaccine treatments. positron emission tomography/computed tomography (pet/ct) has been established as a valuable tool for studying a number of afflictions such as cancer, alzheimer's disease, and inflammation/infection. outlined here are a numb ... | 2017 | 28930979 |
| nucleic acid polymers with accelerated plasma and tissue clearance for chronic hepatitis b therapy. | rep 2139 is a nucleic acid polymer (nap) currently under clinical development for chronic hepatitis b (hbv) therapy. this preclinical study investigated different rep 2139 analogs that would display reduced accumulation in the serum and tissues, while retaining an antiviral effect against hbv infection. rep 2139 analogs were evaluated in human plasma, cd-1 mice, cynomolgus monkeys, and pekin ducks. discrete ribose transformation to 2'oh in selected riboadenosines resulted in a slow degradation i ... | 2017 | 28918011 |
| potential effect of prolonged sevoflurane anesthesia on the kinetics of [11c]raclopride in non-human primates. | positron emission tomography (pet) in non-human primates (nhp) is commonly performed under anesthesia, with sevoflurane being a widely used inhaled anesthetic. pet measurement in nhp can be repeated, and a difference in radioligand kinetics has previously been observed between the first and second pet measurement on the same day using sevoflurane anesthesia. in this study, we evaluated the effect of prolonged sevoflurane anesthesia on kinetics and binding potential (bpnd) of [11c]raclopride in n ... | 2018 | 28916921 |
| effects of icos+ t cell depletion via afucosylated monoclonal antibody medi-570 on pregnant cynomolgus monkeys and the developing offspring. | medi-570 is a fully human afucosylated monoclonal antibody (mab) against inducible t-cell costimulator (icos), highly expressed on cd4+ t follicular helper (tfh) cells. effects of medi-570 were evaluated in an enhanced pre-postnatal development toxicity (eppnd) study in cynomolgus monkeys. administration to pregnant monkeys did not cause any abortifacient effects. changes in hematology and peripheral blood t lymphocyte subsets in maternal animals and infants and the attenuated infant igg immune ... | 2017 | 28916434 |
| multilocus genotyping of giardia duodenalis in captive non-human primates in sichuan and guizhou provinces, southwestern china. | giardia duodenalis is a common human and animal pathogen. it has been increasingly reported in wild and captive non-human primates (nhps) in recent years. however, multilocus genotyping information for g. duodenalis infecting nhps in southwestern china is limited. in the present study, the prevalence and multilocus genotypes (mlgs) of g. duodenalis in captive nhps in southwestern china were determined. we examined 207 fecal samples from nhps in sichuan and guizhou provinces, and 16 specimens wer ... | 2017 | 28910395 |
| longitudinal multiparameter single-cell analysis of macaques immunized with pneumococcal protein-conjugated or unconjugated polysaccharide vaccines reveals distinct antigen specific memory b cell repertoires. | the efficacy of protein-conjugated pneumococcal polysaccharide vaccines has been well characterized for children. the level of protection conferred by unconjugated polysaccharide vaccines remains less clear, particularly for elderly individuals who have had prior antigenic experience through immunization with unconjugated polysaccharide vaccines or natural exposure to streptococcus pneumoniae. | 2017 | 28910279 |
| evaluation of infrared thermometry in cynomolgus macaques (macaca fascicularis). | recording an accurate body temperature is important to assess an animal's health status. we compared temperature data from sedated cynomolgus macaques (macaca fascicularis) to evaluate differences between rectal, infrared (inguinal and chest), and implanted telemetry techniques with the objective of demonstrating the diagnostic equivalence of the infrared device with other approaches. infrared thermometer readings are instantaneous and require no contact with the animal. body temperature data we ... | 2017 | 28905720 |