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bee - keepers' dermatitis - sensitivity to propolis.a patient working in the bee-keeping department developed contact dermatitis on.201728128148
serial studies on the functional affinity and heterogeneity of antibodies of different igg subclasses to phospholipase a2 produced in response to bee-venom immunotherapy.high functional affinity and high titer igg4 antibodies to phospholipase a2 were produced by allergic patients in response to bee-venom immunotherapy. in contrast, the affinity of igg1 antibodies decreased after immunotherapy, and both the titer and affinity of igg1 antiphospholipase a2 remained significantly lower compared to igg4 1 to 2 years after treatment. analysis of affinity heterogeneity suggested a loss of igg1 high-affinity antibody-producing clones during immunotherapy and a preferent ...19892778238
[the use of the products of bee raising in medicine]. 19892591547
[preparation of highly-purified melittin from bee venom by an affinity chromatography method].the method of melittin (a lytic peptide from bee venom) purification is described. the method is based on application of immunosorbent with antibodies against phospholipase a2 and permits obtaining peptide without the residual phospholipase activity. it can be also used for the phospholipase a2 purification from melittin admixtures.20152588352
relevance of basophil histamine release changes during venom immunotherapy.we investigated the effect of rush and long-term venom immunotherapy on histamine release parameters in bee venom allergic patients. ten patients received rush venom immunotherapy, and histamine release data were obtained immediately before and after treatment. 17 patients were assessed by histamine release 24 to 63 months after termination of long-term venom immunotherapy. a control group of 10 non-allergic subjects was included in this study. histamine released from whole blood was determined ...19892479278
mast cell degranulating peptide forms voltage gated and cation-selective channels in lipid bilayers.mast cell degranulating peptide, a toxin of bee venom, is a polypeptide composed of 22 amino acids. exposure of the asolectin bilayer to the peptide results in the formation of channels that are more permeable to k+ than cl-. these channels are activated when the voltage of the cis side, to which the peptide is added, is made positive.19892476125
predictive value of venom-specific ige, igg and igg subclass antibodies in patients on immunotherapy with honey bee venom.sixty-seven patients with a history of severe systemic reactions following honey bee stings were treated by immunotherapy (it) with honey bee venom. during maintenance therapy all were submitted to a sting challenge under clinical conditions. 15 developed mostly minor symptoms of a systemic reaction while 52 showed only a local swelling at the sting site. phospholipase a2-specific ige, igg and igg subclass serum antibodies were estimated in samples obtained before it and immediately before the c ...19892802114
histochemistry of acetylcholinesterase and immunocytochemistry of an acetylcholine receptor-like antigen in the brain of the honeybee.a histochemical staining method for acetylcholinesterase (ache) and an antiserum raised against nicotinic acetylcholine receptors (achr) of locust nervous tissue were applied in order to reveal certain candidates of cholinergic pathways in the brain of the honeybee. the ache staining marked layers in the optic lobes, fibers connecting the two brain hemispheres, and fiber tracts as well as soma clusters within the protocerebrum. the calycal input regions of the mushroom bodies were labelled, wher ...19892768558
[allergy to hymenoptera venom in children. diagnosis and therapy].we studied 52 children, 3 to 15 years of age, suffering from allergic reactions following hymenoptera stings. the diagnosis of allergy was based on history, results of skin tests and rast performed by purified venoms of apis mellifera, vespula species and polistes species. the history of children with multiple stings of the same insect showed an essential favourable prognosis, thus suggesting to submit to specific immunotherapy only children with severe reactions. for these patients it is necess ...19892601661
[bee and wasp venom allergy].since allergic reactions to insect venom can become life-threatening, accurate diagnosis and proper treatment are important. the diagnosis of insect venom allergy is made on the basis of the history, a skin test taking the form of an intradermal titration series, and serodiagnosis (ige). in proven allergy, the patient must be informed about measures to be taken to avoid being stung. furthermore, the patient will have always to carry a "shock kit" comprising an antihistaminic, a steroid and adren ...19892670712
[bee husbandry products in the treatment of diseases of the digestive organs]. 19892796680
efficacy of tube feeding in supplying energy requirements of hospitalized patients.during a 6-week period, all adult patients in a university hospital receiving ready-to-feed nasoenteric tube feeding formula were prospectively studied. the study objective was to determine each patient's caloric intake from tube feeding relative to their energy needs and to identify factors causing decreased feeding intake. each of 35 patients was visited at least once daily to determine their volumetric intake of tube feeding formula. daily review of patient care records and nursing interviews ...20172506375
a high-sensitivity differential scanning calorimetric study of the interaction of melittin with dipalmitoylphosphatidylcholine fused unilamellar vesicles.high-sensitivity differential scanning calorimetry has been used to examine the interaction of bee venom melittin with dipalmitoylphosphatidylcholine fused unilamellar vesicles. experiments were performed under conditions for which melittin in solution is either monomeric (in low salt) or tetrameric (in high salt). it was found that under both sets of conditions melittin abolishes the pretransition at a relatively high lipid-to-protein molar incubation ratio, ri (about 200) and that at intermedi ...19892472839
volatiles of two chemotypes of majorana syriaca l. (labiatae) as olfactory cues for the honeybee.a study of olfactory discrimination by honeybee has been set up in order to understand the role of volatiles of the aromatic plant majorana syriaca in attracting pollinating insects. the honeybee's response to volatiles from leaves and inflorescences of two m. syriaca chemotypes, which differ in the thymol carvacrol ratio of their volatiles, was tested using a bioessay method based on associative conditioning and recruitement techniques. behavioural data show that a honeybee identifies and react ...198928313476
alarm pheromone production by two honeybee (apis mellifera) types.of 12 alarm pheromones assayed in european and africanized honeybees, nine were found in larger quantities in the africanized population. isopentyl and 2-heptanone levels were similar in both; 2-methylbutanol-1 was greater in european workers. these differences were not due to age or geographical location. significant positive correlations between alarm pheromone levels and defensive behavior, especially numbers of stings, were observed.198924272178
the rate of sulfur hexafluoride escape from a plastic syringe.sulfur hexafluoride (sf6) gas is widely used for internal tamponade during retinal reattachment surgery and is commonly injected into the eye from a 10-ml plastic syringe. the rate of diffusion of sf6 out of a plastic syringe has not bee studied. we measured the percentage of sf6 gas in a 10-ml plastic syringe by gas chromatography, confirmed by infrared spectrometry. measurements were obtained immediately after aspiration, and at 30 s and 10, 15, 60, 90, and 120 minutes, and 18 hours. a marked ...19892730405
the formamidine amitraz as a hyperglycemic alpha-agonist in worker honeybees (apis mellifera mellifera l.) in vivo.intra-abdominal injection of amitraz (0.25 nmol per honeybee, i.e., approx 2.3 nmol/g) to emerging worker bees, in vivo, led to a significant hypertrehalosemia (300-400%) followed by a hyperglucosemia (approximately equal to 600%). maxima were reached at 0.5 and 2 h, respectively. a strong negative correlation between glucosemia and trehalosemia appeared after injection of pure phentolamine (1 nmol per bee), suggesting stimulation of trehalase activities. simultaneous administration of the alpha ...19892574043
evidence from mitochondrial dna that african honey bees spread as continuous maternal lineages.african honey bees have populated much of south and central america and will soon enter the united states. the mechanism by which they have spread is controversial. africanization may be largely the result of paternal gene flow into extant european populations or, alternatively, of maternal migration of feral swarms that have maintained an african genetic integrity. we have been using both mitochondrial and nuclear dna restriction fragment length polymorphisms to follow the population dynamics b ...19892566122
[ca-inhibited binding of melittin with parvalbumin. a new role for parvalbumins?].it was found that pike parvalbumins pi 4.2 and 5.0 bind amphiphilic peptide melittin extracted from bee venom in an extraordinary ca-dependent manner: in apo-state the protein forms a tight equimolar complex with melittin (ka = 10(6) m-1 at 18 degrees c); in ca- (and mg-) loaded state it does not take place. heating of the protein up to temperatures above the denaturation temperature of apo-parvalbumin does not change the stoichiometry of the complex but increases its association constant by an ...20132770738
anaphylactic shock occurring outside hospitals.in a retrospective study, all cases of anaphylactic shock (as) occurring in a hospital catchment area during a 13-year period were analysed. twenty cases were found, giving an incidence of 3.2 cases per 100,000 inhabitants per year, and one patient died. the precipitating agents were penicillin (7 cases), aspirin (3), food (2), and bee or wasp sting (8). the drugs had all been given orally. penicillin-related as was much commoner than its incidence reported by the danish national health authorit ...19892735517
arrangement of filaments and cross-links in the bee flight muscle z disk by image analysis of oblique sections.information from oblique thin sections and from three-dimensional reconstructions of tilted, transverse thin sections (cheng, n., and j. f. deatherage. 1989. j. cell biol. 108:1761-1774) has been combined to determine the three-dimensional structure of the honeybee flight muscle z disk at 70-a resolution. the overall symmetry and structure of the z disk and its relationship to the rest of the myofibril have been determined by tracing filaments and connecting elements on electron images of obliqu ...19892715177
three-dimensional reconstruction of the z disk of sectioned bee flight muscle.the three-dimensional structure of the central region of the z disk of honeybee flight muscle has been determined to a resolution of 70 a by three-dimensional reconstruction from electron micrographs of tilted thin sections. the reconstructions show a complex assembly in which actin filaments terminate and are cross-linked together; a number of structural domains of this network are resolved in quantitative three-dimensional detail. the central region of the z disk contains two sets of overlappi ...19892715176
[insect sting allergy--clinical aspects, diagnosis and therapy].systemic allergic reactions following insect stings, most often by hymenoptera (honey bee, wasp), are usually of the immediate type and thus mediated by venom specific ige-antibodies. the most frequent clinical symptoms are: urticaria, angioedema, asthma and anaphylactic shock. while up to 4% of individuals in epidemiological surveys give a history of systemic allergic reactions following hymenoptera sings, fatal cases are extremely rare. diagnosis is based on history, skin test and estimation o ...19892658345
hypereosinophilia, neurologic, and gastrointestinal symptoms after bee-pollen ingestion.a patient developed hypereosinophilia (13,440 cells per cubic millimeter) 6 weeks after beginning the ingestion of bee pollen. symptoms included generalized malaise, headache, nausea, abdominal pain diarrhea, generalized pruritus, and decreased memory. evaluation revealed no other known cause for the patient's hypereosinophilia, which resolved after bee-pollen ingestion was stopped. the product contained a mixture of entomophilous and anemophilous pollens to which the patient was skin test posit ...19892708739
amplification of small signals by voltage-gated sodium channels in drone photoreceptors.photoreceptor cells of the drone, apis mellifera male, have a voltage-gated na+ membrane conductance that can be blocked by tetrodotoxin (ttx) and generates an action potential on abrupt depolarization: an action potential is triggered by the rising phase of a receptor potential evoked by an intense light flash (autrum and von zwehl 1964; baumann 1968). we measured the intracellular voltage response to a small (9%), brief (30 ms) decrease in light intensity from a background, and found that its ...19892555482
concomitant augmentation of cd4+ cd45r+ suppressor/inducer subset and diminution of cd4+ cdw29+ helper/inducer subset during rush hyposensitization in hymenoptera venom allergy.a longitudinal study of patients undergoing rush hyposensitization by honey-bee or yellow jacket venom revealed significant changes of the immunophenotypes until the optimal dose was reached, and a progressive reversion to pre-treatment values in the following months. the activation markers cd23 on b cells and cd25 (il-2 receptor) on t and b lymphocytes decreased. although there was little variation of the major cd4 and cd8 lymphocyte populations, cd45r+ cells increased whilst cdw29+ lymphocytes ...19892525435
allosteric inhibition of nicotinic acetylcholine receptors of vertebrates and insects by philanthotoxin.the effects of pure philanthotoxin (phtx), a component of the venom of the wasp philanthus triangulum, were studied on nicotinic acetylcholine receptors (nachrs) of vertebrates and insects so as to compare their sensitivities and the mechanism of action of phtx. electrophysiological techniques were used on frog muscles and cockroach thoracic ganglia and biochemical techniques were applied to membranes from torpedo electric organ and honeybee brain. phtx (1-20 microm) inhibited reversibly the ind ...19892468760
the bee situation: africanized honey bees and bee mites. 198917847268
implementation of ternary phase amplitude filters using a magnetooptic spatial light modulator.ternary modulation can be achieved with a single slm and improvedc orrelationd iscriminationc an bee xperimentally realized.198920548613
bee-sting anaphylaxis. 19892716633
bee visitation rates to trees of prockia crucis differing in flower number.annual flower production ranged over four orders of magnitude among individuals of the tropical tree prockia crucis (flacourtiaceae), but the number of visits per flower by bees did not increase with flower number. in a population in jalisco, méxico, the trees flower for about one week each year, offering only pollen to the bees (mostly solitary species). in a small group of trees, the number of visits per flower was less on trees with greater flower production but in a large group visitation di ...198928312586
covariance between relatives for x-chromosomal loci in a population in disequilibrium.according to hardy-weinberg, for a single autosomal locus, a population achieves equilibrium in one generation of random mating if allelic frequency is the same in the sexes, or in two generations if the frequency is not. for a single x-chromosomal locus, however, the approach to equilibrium oscillates and is gradual. covariances between relatives for autosomal and for x-chromosomal loci are in the literature for a random mating population in equilibrium. although assumption of equilibrium is de ...198924232606
inbreeding, coancestry, and covariance between relatives for x-chromosomal loci.knowledge about the relationships between relatives for x-chromosomal loci is necessary to compute genetic variances and covariances for the genetic evaluation of individuals for economically important traits in livestock and poultry. using a unified approach, we derived explicitly the coefficient of inbreeding for individuals and the coefficient of coancestry between collateral and lineal relatives of the same or different sex, assuming that the male is heterogametic and the female is homogamet ...20082926116
local mediator release in bee venom anaphylaxis. 19892752323
natural history of insect sting allergy: the rhode island experience.the frequency of insect sting allergy in a given population varies depending on the age group of the population and the type of criteria used (history, skin test or rast). in a pediatric population with a mean age of 13 years (boy scouts), the frequency is 0.8% using history as the only criteria. with this same criteria in an adult population, other authors found the frequency to be higher, 3.3%. the difference is probably due to the prolonged exposure rate to insect stings in adults. using skin ...20042737467
hymenoptera venom immunotherapy. i. induction of t cell-mediated immunity by honeybee venom immunotherapy: relationships with specific antibody responses.the specific cell-mediated and humoral immune responses of 14 children allergic to honeybee venom were studied. an 8-day rush venom immunotherapy induced an increase in t proliferative (p less than 0.04) and t suppressive (p less than 0.003) cell-specific activities. antibody variations, an increase in specific igg4 (p not equal to 0.05), and a decrease in specific ige (p less than 0.01) were observed 1 year later. initial high t suppressive cell activity prevents t proliferative cell increase d ...19892522473
lethality of 'killer' bee stings. 19892915687
dopamine-like immunoreactivity in the brain and suboesophageal ganglion of the honeybee.the distribution of dopamine in the brain and suboesophageal ganglion of the honeybee apis mellifera was investigated by means of immunocytochemistry with a well-characterized antiserum against dopamine. the binding of the antiserum in paraffin serial sections was studied with the peroxidase-antiperoxidase method. dopamine-like immunoreactive neurons are present in most parts of the brain and in the suboesophageal ganglion. only the optic lobes are devoid of label. there are ca. 330 dopamine imm ...19892918095
sensory pathways and motoneurons of the proboscis reflex in the suboesophageal ganglion of the honey bee.a morphological description is given for the motoneurons underlying the proboscis extension reflex in the honey bee (apis mellifera). sensory neurons from the mandibles and labium were examined and their projection areas were related to those of the motoneurons. motoneurons are located in the ventral part of the suboesophageal ganglion, have their somata in the hemiganglion ipsilateral to the filled nerve, and send prominent branches through identified ventral commissures into the contralateral ...19892918084
age-related changes in aggression in ant cataglyphis cursor (hymenoptera, formicidae): influence on intercolonial relationships.cataglyphis cursor forms monogynous societies which may however accept some foreign viduals. the factors influencing the degree of aggressiveness of the workers have been studied as follows. we have shown that the aggressive behaviour is expressed mainly by the foragers, the nurses are very little. aggressive. we observe therefore an evolution in the reactivity of the workers to a foreign odour in terms of age-related changes. however the closing of the society still depends on the behaviour of ...198924897675
sunflower volatiles involved in honeybee discrimination among genotypes and flowering stages.in order to define the part of olfactory cues in the selective behavior of honeybees, observation on their foraging behavior was carried out on various sunflower genotypes in parallel with chemical analysis of aromatic extracts of the genotypes. foragers show a preference for the early stages of flowering and, when they are given a choice between couples of parental lines of two commercial hybrids, marianne and mirasol, they are randomly distributed on mirasol parents, but they prefer the female ...198924271447
intercellular junctions and rhombic particle arrays in the developing and adult dorsal ocelli of the honeybee.intercellular junctions and particle arrays in the developing and mature dorsal ocelli of the honeybee apis mellifera have been studied with conventional and freeze-fracture electron microscopy. four types of junctions are found in the lentigenic and retinogenic part during development. these are desmosomes, septate junctions, tight junctions, and gap junctions. gap junctions and septate junctions are found between differentiating photoreceptor cells only as long as the rhabdoms are beginning to ...198918620260
laboratory investigations of the electrical characteristics of honey bees and their exposure to intense electric fields.bees exposed to 60-hz electric (e) fields greater than 150 kv/m show field-induced vibrations of wings, antennae, and body hairs. they also show altered behavior if exposed while in contact with a conductive substrate. measurements indicate that approximately 240 na is coupled to a bee standing on a conductive substrate in a 100-kv/m e field. in lab experiments, bee disturbance and sting result from exposure to e field greater than 200 kv/m (bee current greater than 480 na) and reduced voluntary ...19892712835
[varroatosis and methods for its control].since 1977 varroatosis has spread over the whole federal republic of germany. this mite infection of bees, which is very new in europe, has already killed many swarms and could not be stopped by veterinary regulatory measures (notifiability, restricted movements, obligatory treatment). at present bee-keepers are trying to avoid losses by a number of biologic measures and medications. such measures should be based on exact knowledge of the parasite and its behaviour in the bee population. the pre ...19892669231
energy expenditure and the abnormal eating pattern of a bulimic: a case report.bulimia nervosa has been characterized by binge eating, which is often followed by either vomiting or laxative use. however, some bulimic individuals restrict caloric intake rather than purge as a means of controlling their weight. treatment may include psychiatric evaluation, drug therapy, and nutrition counseling. this study evaluated the eating pattern of a bulimic woman from food records made before and while she was receiving nutrition counseling at an eating disorders clinic. energy expend ...19892642496
chloride enters glial cells and photoreceptors in response to light stimulation in the retina of the honey bee drone.double-barrelled ion-selective microelectrodes were used to measure free [cl-] in photoreceptors, extracellular space, and glial cells in superfused slices of drone retina. tests indicated that with normal superfusate the intracellular electrode signal was due essentially to cl- and not to some other interfering anion. the results indicate that cl- is more concentrated in both photoreceptors and glial cells than would be predicted for a passive electrochemical distribution. when the photorecepto ...19892530169
functions of glial cells in the retina of the honeybee drone.in the retina of the honey bee drone, apis mellifera male, physiological interactions between glial cells and neurons (the photoreceptors) are exceptionally clear-cut and amenable to investigation. the principal glia (outer pigment cells) contribute to the homeostasis of extracellular [k+] and [na+] by 1) spatial buffering of k+ and 2) net uptake of k+ and cl-. the glia supply carbohydrate metabolic substrate to the neurons; only the glia take up and phosphorylate glucose. neuronal activity 1) m ...19892523335
use of total parenteral nutrition in pediatric bone marrow transplantation.to evaluate the extent of the nutritional stress of pediatric bone marrow transplantation (bmt) and to evaluate the use of total parenteral nutrition (tpn), 35 consecutive pediatric patients who received bmt were studied retrospectively. voluntary cessation of oral nutrition in almost all patients was observed, and significant decreases of serum albumin levels were seen after bmt. in 85% of these patients, tpn was necessary in response to severe wasting and fasting. no deaths were related to ind ...20072520253
the influence of time of day on the foraging behavior of the honeybee, apis mellifera.the honeybee time sense, or zeitgedächtnis, is highly adaptive, allowing bees to synchronize their foraging behavior with the peak time of daily floral nectar rhythms. each foraging group within the honeybee colony shows a high degree of fidelity to one species of flower. across the day, the temporal accuracy of foraging visits to experimental feeding times varies considerably, being nearly exact for morning-trained foraging groups but becoming less so for foraging groups trained later in the da ...19892519596
analysis of differing patterns of cross-reactivity of honeybee and yellow jacket venom-specific ige: use of purified venom fractions.prior studies of sera from insect sting-allergic patients have analyzed the relationship of coexisting honeybee venom- and yellow jacket venom-specific ige. radioallergosorbent (rast)-inhibition tests with these venoms revealed four different patterns of activity. in this present study, purified fractions prepared from these venoms were used to analyze these varying patterns. the hyaluronidases of yellow jacket venom and honeybee venom showed extensive cross-reaction. the phospholipases from the ...19892499550
characterization of the honeybee haemolymph alpha-glycosidase specificity and apparent cooperativity as related to substrate-directed aggregation of enzyme asymmetric protomers.based on experimentally determined glycosidase molecular forms, their specificity and apparent hill coefficients against trehalose (1.4) and sucrose (0.6), respectively, in honeybee haemolymph, a theoretical model is proposed involving differential types of non-random aggregation of a single enzyme protomer. this basic unit contains one trehalose-specific site and two asymmetrical subsites: one holds a catalytic zone and both share a proper affinity to any substrate non-specific binding zone. th ...19892487009
antibodies to the mammalian adhesion molecule j1/tenascin and its carbohydrate epitope l2/hnk-1 label the receptor lymph cavities of insect sensilla.data from light- and electronimmunocytochemistry gave evidence that the antibodies to the mammalian adhesion molecule j1/tenascin and its carbohydrate structure l2/hnk-1 react with immunoreactive structures present in the inner and outer receptor lymph cavities of antennal sensilla of the honey bee. immunoreactivity was additionally present in the cytoplasm of the enveloping cells surrounding the receptor lymph cavities. cell contacts between enveloping cells and between dendrites and enveloping ...19892482552
management of bee-sting anaphylaxis.a retrospective case analysis of 101 adverse reactions to bee-stings and a prospective questionnaire analysis of the proposed management by local medical practitioners and resident hospital staff members of three hypothetical bee-sting reactions has revealed that understanding of the use of adrenaline in patients with reactions to bee envenomation is confused with regard to the indications for its use, dosage and route; that corticosteroid agents are used or are recommended too frequently, somet ...20153200185
"bee-sting kills mother of 10". 20133200180
eclectic chemisociality of the honeybee : a wealth of behaviors, pheromones, and exocrine glands.a dazzling variety of honeybee behaviors are triggered by pheromones produced by disparate exocrine glands. a multiplicity of chemical releasers of social behavior has been demonstrated to regulate a diversity of societal interactions, and many of these compounds are synthesized with great caste specificity. recent investigations have resulted in the identification of a host of new compounds that are products of either worker or queen honeybees. this report fractionates these newly identified ex ...198824277146
comb wax mediates the acquisition of nest-mate recognition cues in honey bees.honey bees, apis mellifera, acquire nest-mate recognition cues from wax, the predominant material used in nest construction. exposure of a newly emerged worker bee to wax-comb substrate significantly reduced the acceptability of that worker to sister bees. cues acquired from the comb provided colony-specific information about the identity of worker bees; moreover, the effect of comb exposure has been previously shown to override individually produced cues. food odors (anise oil), when dissolved ...198816593995
solution structure of apamin determined by nuclear magnetic resonance and distance geometry.the solution structure of the bee venom neurotoxin apamin has been determined with a distance geometry program using distance constraints derived from nmr. twenty embedded structures were generated and refined by using the program dspace. after error minimization using both conjugate gradient and dynamics algorithms, six structures had very low residual error. comparisons of these show that the backbone of the peptide is quite well-defined with the largest rms difference between backbone atoms i ...19883242598
allergens in hymenoptera venom. xx. isolation of four allergens from imported fire ant (solenopsis invicta) venom.commercial solenopsis invicta (sol i) venom was fractionated by gel filtration and high-performance cation exchange chromatography. four proteins were isolated and purified to homogeneity. the four proteins were tested with a panel of sera from patients allergic to fire ant venom; all proteins had significant allergenic activity. these proteins corresponded to four of the bands we previously reported to be allergenic by immunoblot analysis. sol i i has an apparent molecular weight of 37,000 dalt ...19883192865
late onset reactions following venom immunotherapy and venom skin tests.this report describes patients who had late onset reactions following venom immunotherapy and venom skin tests. six adult patients had symptoms of fatigue, malaise, fever, headache, and joint ache, starting four to six hours after venom immunotherapy and lasting up to four days. two of the patients had prolonged reactions at or adjacent to the skin test sites. all of these patients had a history of venom anaphylaxis; four had severe cardiovascular symptoms. all received yellow jacket venom immun ...19883189964
honeybee retinal glial cells transform glucose and supply the neurons with metabolic substrate.the retina of the honeybee drone is a nervous tissue in which glial cells and photoreceptor cells (sensory neurons) constitute two distinct metabolic compartments. retinal slices incubated with 2-deoxy[3h]glucose convert this glucose analogue to 2-deoxy[3h]glucose 6-phosphate, but this conversion is made only in the glial cells. hence, glycolysis occurs only in glial cells. in contrast, the neurons consume o2 and this consumption is sustained by the hydrolysis of glycogen, which is contained in ...19883186756
thermal effects on activity patterns and behavioural switching in a concourse of foragers on stachytarpheta mutabilis (verbenaceae) in papua new guinea.1. the foraging activities of the papilionid butterflies ornithoptera priamus poseidon and papilio ulysses, and the solitary bee amegilla sapiens (apoidea, anthophoridae) on the shrub stachytarpheta mutabilis were studied in highland papua new guinea. 2. the insects' activity patterns were analysed at three sites with differing diurnal microclimate variation. o. priamus and a. sapiens foraged in the morning (after a period of basking and wing-whirring) and late afternoon when temperatures were w ...198828312315
kinetics of oxygen consumption after a flash of light in the lateral ocellus of the barnacle.until recently, polarographic methods for measuring the time course of transient changes in the rate of oxygen consumption (deltaqo(2)) have been applied only to tissue preparations containing thousands of cells. here, we describe deltaqo(2) measurements on the lateral ocellus of the barnacle (balanus eburneus) which contains only three photoreceptor cells. the decrement of partial pressure of oxygen (deltapo(2)) elicited by an 80 ms flash of light was measured near the cells with a microelectro ...198819431731
inactivation of phospholipase a2 by manoalide. localization of the manoalide binding site on bee venom phospholipase a2.the marine natural product manoalide (mld), a potent inhibitor of phospholipases, completely inactivates bee venom phospholipase a2 (pla2) by an irreversible mechanism. it has been proposed [k. b. glaser and r. s. jacobs, biochem. pharmac. 36, 2079 (1987)] that the reaction of mld with pla2 may involve the selective reactivity of mld to a peptide sequence, possibly a lys-x-x-lys peptide. localization of the mld binding site on bee venom pla2 demonstrated that upon mld modification of bee venom p ...19883178877
[the basophilic histamine liberation test as a diagnostic method in hymenoptera venom allergy].in 181 patients with systemic reactions to hymenoptera stings as a result of immediate-type allergy to bee or wasp venom confirmed by history, skin testing and demonstration of hymenoptera venom-specific ige antibodies on radioallergosorbent testing (rast), in vitro histamine release from peripheral basophilic granulocytes following stimulation with bee or wasp venom was evaluated. suspensions of washed peripheral leukocytes at a density of 2 x 10(6)/ml were incubated with four different concent ...19882466814
representation of conspecific song by chickadees: comparisons among embedded 'fee bees'.captive black-capped chickadees (parus atricapillus) were presented with normal (2-note) and lengthened (3-note) songs to investigate how note number and order are represented. normal fee bee (fb) song was embedded in four different 3-note sequences containing an additional fee (f) or bee (b). latency to vocalize after playback varied with the position, but not the type of added note, (i.e. slower responses to bfb and ffb than to fbf and fbb). latency did not differ appreciably between normal (2 ...198824897546
the oldest fossil bee: apoid history, evolutionary stasis, and antiquity of social behavior.trigona prisca, a stingless honey bee (apidae; meliponinae), is reported from cretaceous new jersey amber (96-74 million years before present). this is about twice the age of the oldest previously known fossil bee, although trigona is one of the most derived bee genera. t. prisca is closely similar to modern neotropical species. most of bee evolution probably occurred during the approximately 50 million years between the beginning of the cretaceous when flowering plants (on which bees depend) ap ...198816593976
[hypertensive syndrome after bee and wasp stings]. 19883204952
should x-rays be ordered to find a bee's stinger?on several occasions, we have been asked to locate bee stingers so that they could be removed. the studies were negative, and we did not know if identification was even possible. an experiment was done using both film screen studies and xerograms at various techniques to attempt to locate a honey bee stinger. the stinger could not be identified.19883186527
the hymenoptera venom study i, 1979-1982: demographics and history-sting data.the hymenoptera venom study, a study based on case histories, skin test results and adverse reactions, immunotherapy and adverse reactions, and treatment efficacy, for 3236 hymenoptera-allergic subjects, was begun in 1979 after the food and drug administration approval of hymenoptera venoms. eighty-four fellows and members of the american academy of allergy and immunology participated in the study. all subjects had a history of an adverse reaction to one or more hymenoptera insects. the mean age ...19883170986
[the honeybee (apis mellifera) as an indicator of lead and cadmium levels in two locations]. 19883053102
prolonged interval maintenance venom immunotherapy.current guidelines recommend indefinite administration of venom immunotherapy at 4-week intervals. to simplify this therapy, we examined the clinical and immunologic response after extending the interval between maintenance venom injections to 6, 8, and 12 weeks. twenty-six patients who had had sting anaphylaxis and positive skin tests received maintenance bee or yellow jacket venom immunotherapy at greater or equal to 6-week intervals. this extended interval was started after a prior average 4- ...19882458060
temperature-dependent shift of fluorescence spectra without conformational changes in protein; studies of dipole relaxation in the melittin molecule.when studying bee venom melittin in an ordered tetrameric form we found a shift of the fluorescence spectrum to a longer wavelength with a rise in temperature above 25 degrees c. the application of the methods of circular dichroism, temperature-perturbation difference spectrophotometry, gel filtration, ionic quenching and polarization of trp-19 fluorescence argues against the possibility of dissociation and change in conformation with the rise in temperature. the spectral shifts are, probably, c ...19883401493
the role of insect water balance in pollination ecology: xylocopa and calotropis.two carpenter bees (xylocopa spp.) in southern israel both use the asclepiad calotropis procera as a primary nectar source. this plant genus is coevolved with carpenter bees, and aspects of the insect-flower interaction in israel suggest that the smaller bee, x. sulcatipes, is the natural co-adapted pollinator, a view borne out by the geographical distributions of the species concerned. there are significant mismatches between the plant and the larger x. pubescens, involving physical fit and beh ...198828312024
in situ localization of the transcripts of a homeobox gene in the honeybee apis mellifera l. (hymenoptera).we have isolated and characterized a homeoboxcontaining gene from the honeybee apis mellifera. its homeobox region shows a high degree of sequence similarity to the homeobox of the drosophila gene deformed (dfd). at the dna level 82% of the basepairs are the same, whereas the putative amino acid sequences are identical between the bee and the fruitfly genes. similarity is also present 5' and 3' to the homeobox. using this isolate as a probe we have performed in situ hybridization on sections fro ...198828305786
structure of p401 (mast cell degranulating peptide) in solution.p401 (also known as mast cell degranulating protein, mcd) is a minor component of honeybee venom. its primary structure is related to that of apamin. we have studied the structure of p401 in solution by high-resolution two-dimensional 1h-nmr spectroscopy. almost all the backbone proton resonances have been assigned by sequential assignment strategy. analysis of noes shows that p401 has a conformation very similar to that of apamin. n-terminal residues ile-1-cys-5 are in an extended conformation ...19883233281
spin-label assay for phospholipase a2.a rapid and continuous method for measuring phospholipase a2 activity using electron spin resonance spectroscopy and a spin-labeled phospholipid as a substrate has been developed. the substrate, 1-palmitoyl-2-(4-doxylpentanoyl)glycerophosphocholine, gives rise principally to a broad esr line in aqueous solution due to strong spin-spin interactions, probably resulting from its micellar formation. upon addition of bee venom phospholipase a2, the water-soluble product, 4-doxylpentanoic acid, is rel ...19882847584
electrocardiographic changes following bee-sting anaphylaxis. 19883411592
systemic reactions during maintenance immunotherapy with honey bee venom.immunotherapy with hymenoptera venoms is safe and effective in most patients but treatment failures have been reported. five patients experienced systemic symptoms of anaphylaxis when they were in maintenance immunotherapy with honey bee venom. in one case, the patient presented a severe life-threatening reaction when stung by a honey bee. three others had the development of new clinical sensitivity suggesting a re-sensitization. this occurred in the fifth patient after a severe viral infection. ...19883389573
the ontogeny of kin discrimination cues in the honey bee, apis mellifera. 19883190638
kin recognition in the sweat bee, lasioglossum zephyrum. 19883190637
social plasticity and early-diapausing females in a primitively social bee.many of the females of the first summer brood of the sweat bee halictus rubicundus mate, soon vanish from the nesting site, undergo diapause, and return unworn the following spring to found their own nests without ever functioning as workers. roughly half a season's foundresses may originate in this way. this finding contradicts previous assumptions regarding timing of reproductive production in sweat bees and offers a remarkable example of intraspecific social diversity; some females in the pop ...198816593945
[effect of nutritional treatment of esophageal cancer patients on pre and post-operative hemodynamic changes and postoperative lung complications].in 35 patients with esophageal cancer, preoperative nutritional state and hemodynamic changes on exercise test using swan-ganz catheter were studied. thirty-five patients were divided into two groups. group 1; preoperative daily calorie intake was less than 1.75 x bee (basal energy expenditure), group 2; preoperative daily calorie intake was more than 1.75 x bee. there were significant differences between group 1 and group 2 in preoperative daily calorie intake, serum transferrin, retinal bindin ...19883185468
glutamate-like immunoreactivity in identified neuronal populations of insect nervous systems.glutamate is considered to be the most likely transmitter candidate at excitatory synapses onto skeletal muscles of insects. we investigated the distribution of glutamate-like immunoreactivity (glu-li) in identified motor neurons of glutaraldehyde-fixed metathoracic ganglia of the locust in paraffin serial sections. the presumably glutamatergic fast and slow extensor tibiae motor neurons show glu-li, whereas other cells, including the gabaergic common inhibitory motor neurons and the cluster of ...19882898517
severe anaphylactic reactions outside hospital: etiology, symptoms and treatment.twenty-seven patients with severe anaphylactic reactions were treated by two anesthesiologist-staffed ambulance helicopters during a 5-year period. all patients had signs of respiratory and/or circulatory failure. cutaneous symptoms were described in 70% of the patients, gastrointestinal symptoms in 30% and central nervous symptoms in 85%. wasp sting and drugs were the most common causes. other etiologies were bee sting, snakebite, nuts and exercise. all patients were provided with advanced pre- ...19883394485
synthesis of two allergenic constituents of propolis and poplar bud excretion.the prenyl ester and the phenylethyl ester of caffeic acid, formed in the bud excretion of poplar species, were shown recently to be the major contact allergens in bee-glue. an unambiguous synthesis of these compounds, based on the reaction of caffeic acid with 1-bromo-3-methyl-2-butene and with beta-bromoethylbenzene, respectively, is reported. the synthetic products confirm the previously described structures of the natural products and allow further testing of their allergenic properties.20132458657
[nutritional support for preoperative patients with esophageal cancer].nutritional support for patients undergoing major operations seems to be very important for improving the operative results, especially in preoperative patients with conditions such as esophageal carcinoma. in our department, patients who have lost more than 10% of their normal weight have been given intravenous hyperalimentation for at least two weeks preoperatively to prevent postoperative pulmonary complications. however good responses to nutritional support were not always obtained in these ...19883389828
the use of quetol 651 for the post-embedding immunohistochemical demonstration of gamma-aminobutyric acid on semithin sections.quetol 651 was used as an embedding medium for the demonstration of gamma aminobutyric acid (gaba) in semithin sections by the peroxidase-anti-peroxidase method. in order to demonstrate the immunoreactivity, the embedding medium was partially dissolved using absolute ethanol containing 0.8-1 m naoh or koh for 5-7 min. the experimental procedure was elaborated by testing the gabaergic sites in the endings surrounding the small neurones of the anterior exterolateral nucleus of a mormyrid fish and ...19883209422
bee disease prevention. 19883381433
o-aminoacetophenone, a pheromone that repels honeybees (apis mellifera l.).o-aminoacetophenone is a pheromone produced by virgin honeybee queens and released with feces. in small social groups, the pheromone repels and is used to terminate agonistic interactions between queens and workers.19883350140
polymorphisms in mitochondrial dna of european and africanized honeybees (apis mellifera).this study demonstrates polymorphisms in both the length and in the restriction enzyme cleavage sites of honeybee mitochondrial dna (mtdna). the levels of variation are typical of those found in other metazoan species. these polymorphisms are potentially useful for the identification of africanized bees in the western hemisphere and for study of honeybee phylogenetics.19882895016
synthesis of carrier-free tritium-labeled queen bee pheromone.a short synthesis of [4,5-(3)h2] (e)-9-oxo-2-decenoic acid (oda), a high-specific-activity tritium-containing isotopomer of the queen bee pheromone, is described. catalytic tritiation of the ketal of ethyl 9-oxo-4-decenoate introduces tritium into two positions, one of which is completely unactivated. subsequent transformation by selenation, oxidation, and hydrolysis affords the labeled 9-oda at >60 ci/mmol. the material is suitable for biochemical studies of binding and catabolism in ovarian, a ...198824276144
self-reliance through fp program.the yogyakarta chapter of the indonesian planned parenthood association (ippa), having been successful in bringing about a realization by the local community of the importance of family planning, has broadened its activities in an effort to further promote family welfare. since 1980, the organization has begun a number of projects designed to increase the income of its client population. given the fact that family planning acceptors of ippa's yogyakarta chapter range from government officials' w ...198812282154
[isolation of modified allergens from bee venom].to obtain modified bee venom (bv) allergens, covalent binding of bv with previously carboxylated polyethylene glycol (peg) has been used. the conjugation of bv and peg has been achieved by means of carbodiimide. thus 4 kinds of the conjugate with bv/peg ratio ranging from 30:1 to 63:1 have been obtained. the study has shown that chemical treatment in the process of this reaction, dialysis and chromatography does not lead to a decrease in the specific activity of bv, while lyophilization produces ...19883389007
bias current modifies the selectivity of liquid membrane ion-selective microelectrodes.a negative bias potential of up to -80 mv applied to the back of a liquid membrane ion-selective microelectrode containing classical "k+" ion-exchanger was found to make it more selective for millimolar concentrations of k+ over micromolar concentrations of choline, tetramethylammonium, tetraethylammonium and 5-hydroxytryptamine. conversely, positive bias potential increased severalfold the sensitivity to micromolar concentrations of these ions while decreasing the sensitivity to k+. an increase ...19883380648
isolation of nuclei from melittin-destabilized cells.melittin, the hydrophobic polypeptide from bee venom, sufficiently destabilizes the plasma membrane of cultured cells to allow cell disruption in the absence of detergents with minimal homogenization. nuclei are thus isolated in high yield with intact nuclear membranes and high transcriptional activity.19883164171
[propolis-induced contact allergy].six patients with contact dermatitis to propolis are reported (3 women, 3 men; mean age 57 years). all three men had eczema on their hands and had acquired their sensitization in the course of bee keeping. the women had been sensitized by propolis contained in ointments of various kinds. upon patch testing all six patients reacted to propolis and four also to peruvian balsam. while ten flavonoids were test-negative, a cinnamic acid ester (1.1-dimethylallyl-3',4'-dihydroxy cinnamic acid ester) is ...19882967807
profound reversible myocardial depression after anaphylaxis.profound myocardial depression developed in 2 patients after severe anaphylactic reactions following the induction of anaesthesia in 1 case and a bee-sting in the other. neither patient had pre-existent cardiac disease. in both patients haemodynamic assessment, radionuclide ventriculography, and two-dimensional echocardiography confirmed the clinical impression of profound systolic myocardial dysfunction. haemodynamic stability was attained by intra-aortic balloon counterpulsation, which was pro ...19882893192
nest plundering allomones of the fire beetrigona (oxytrigona) mellicolor.ten volatile compounds derived from the cephalic glands of the fire beetrigona (oxytrigona)mellicolor were bioassayed for possible allomonal activities facilitating nest plundering. two diketones, (e)-3-heptene-2,5-dione and (e)-3-nonene-2,5-dione, caused the honeybeeapis mellifera to display avoidance behavior and reduced defensive behavior. these diketones are produced in relatively large quantities in fire-bee cephalic glands.198824275996
nutritional support of the chronically ill elderly female at risk for elective or urgent surgery.among the elderly, those at highest risk are the chronically ill, inactive patients. assessing macronutrient requirements and outcome depends on longitudinal studies. seven chronically ill, elderly female patients suffering from persistent infections, were studied monthly, over a 6-month period to determine their protein and energy requirements. calorie and protein requirements were based on clinical status. the results of the nutrition support program were monitored using: weight change, nitrog ...19883343473
basophil histamine release in insect venom allergy.the aim of the present study was to investigate venom-related and venom-non-related immunological reactions in patients stung by bee or wasp. sixteen consecutive patients (7 with local and 9 with systemic reactions) were tested with skin tests, rast and basophil histamine release (bhr) test immediately after the insect sting and 2, 4, and 16 weeks later. no test was useful immediately after the insect sting, the "anergic period". in agreement with earlier findings, the spt was the only allergy t ...19882452581
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