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territorial analysis of aedes aegypti distribution in two colombian cities: a chorematic and ecosystem approach.a territorial analysis of aedes aegypti density was conducted in two colombian cities using an ecosystem and chorematic approach. entomological and behavioral data (by cluster) and information on the urban context were used to analyze the relationship between territorial structures and dynamics and vector density. the results were represented in graphic (chorematic) models. arauca showed higher vector density than armenia. higher density was related to unplanned urbanization, flood-prone areas, ...201525859719
gc-ms analysis of bioactive components and synthesis of gold nanoparticle using chloroxylon swietenia dc leaf extract and its larvicidal activity.a rapid bio-reduction of chloroauric acid (haucl4) was achieved by chloroxylon swietenia dc leaf extract (csle), which resulted in the formation of well dispersed c. swietenia gold nanoparticles (csgnps). the formation of gnps was confirmed by color changes from yellowish green to purple and their characteristic peak at 545 nm. the characterization of synthesized csgnps was made through x-ray diffraction (xrd), fourier transform infrared spectroscopy (ftir), high resolution transmission electron ...201525854160
evaluation of the atraedes™ lure for collection of culex quinquefasciatus in gravid traps.the typical attractant used in gravid trapping of culex quinquefasciatus is an aged infusion of organic materials, which can change in attractiveness over time. a standardized chemical attractant dispenser derived from grass infusion, the atraedes™ lure, has been produced for the surveillance of the dengue vector aedes aegypti. a study using this lure in combination with the centers for disease control and prevention gravid traps was conducted in tanga, tanzania. the addition of the lure to trap ...201525843185
evaluation of a new spraying machine for barrier treatment and penetration of bifenthrin on vegetation against mosquitoes.the effectiveness and penetration of a novel, truck-mounted mist sprayer (3wc-30-4p provided by american longray) was evaluated with bifenthrin in a large, park-like setting with historic floodwater and woodland mosquito populations. efficacy evaluations were determined through adult population collections and excised leaf bioassays. trapping results showed a mean reduction of 77% in mosquito populations in the treated area for 5 sampling events up to 4 wk posttreatment. leaf bioassays revealed ...201525843180
larvicidal activity of naturally occurring naphthalenedione and its structurally related analogs against three mosquito species.the objective of this study was to determine the larvicidal activity of an active compound isolated from cercis chinensis heartwood and its structurally related analogs against 4th-stage aedes aegypti, culex pipiens pallens, and ae. togoi. the larvicidal compound of c. chinensis was isolated with the use of various chromatographic techniques and identified as analogs of 1,4-naphthalenedione. based on the median lethal concentration (lc(50)) values of commercially procured analogs against ae. aeg ...201525843178
laboratory studies of aedes aegypti attraction to ketones, sulfides, and primary chloroalkanes tested alone and in combination with l-lactic acid.the attraction of female aedes aegypti to single compounds and binary compositions containing l-lactic acid and an additional saturated compound from a set of ketones, sulfides, and chloroalkanes was studied using a triple-cage dual-port olfactometer. these chemical classes were studied because of their structural relation to acetone, dimethyl disulfide, and dichloromethane, which have all been reported to synergize attraction to l-lactic acid. human odors, carbon dioxide, and the binary mixture ...201525843177
two novel bioassays to assess the effects of pyrethroid-treated netting on knockdown-susceptible versus resistant strains of aedes aegypti.we describe 2 new mosquito bioassays for use with insecticide-treated netting or other textiles. the 1st is a cylinder bioassay in which a mosquito is forced to contact treated material regardless of where it lands within the bioassay construct. the 2nd is a repellency/irritancy and biting-inhibition bioassay (ribb) in which human arms and breath are used as attractants. mosquitoes have the choice to pass through holes cut in untreated or treated netting to move from a center release chamber int ...201525843176
comparison of bg-sentinel® trap and oviposition cups for aedes aegypti and aedes albopictus surveillance in jacksonville, florida, usa.the bg-sentinel® (bgs) trap and oviposition cups (ocs) have both proven effective in the surveillance of aedes species. this study aimed to determine which of the 2 traps could best characterize the relative population sizes of aedes albopictus and aedes aegypti in an urban section of jacksonville, fl. until 1986, ae. aegypti was considered the dominant container-breeding species in urban northeastern florida. since the introduction of ae. albopictus, ae. aegypti has become almost completely ext ...201525843173
insecticide resistance and metabolic mechanisms involved in larval and adult stages of aedes aegypti insecticide-resistant reference strains from cuba.studies were conducted to compare levels of insecticide resistance and to determine the metabolic resistance mechanisms in larval and adult stages of aedes aegypti from cuba. three insecticide-resistant reference strains of ae. aegypti from cuba were examined. these strains were derived from a santiago de cuba strain isolated in 1997; it was previously subjected to a strong selection for resistance to temephos (san-f6), deltamethrin (san-f12), and propoxur (san-f13) and routinely maintained in t ...201425843136
truck-mounted area-wide application of pyriproxyfen targeting aedes aegypti and aedes albopictus in northeast florida.this study was conducted to determine the efficacy of truck-mounted ultra-low volume applications of pyriproxyfen against aedes aegypti larvae in artificial water containers and wild adult ae. albopictus populations in an urban setting. the study was conducted over a 3.5-month period (june-october 2012), during which 3 pyriproxyfen applications were conducted. beginning 6 wk prior to the 1st pyriproxyfen spray, 10 biogents-sentinel® traps were used each week to survey the adult ae. albopictus po ...201425843135
mosquito vector biology and control in latin america-a 24th symposium.the 24th annual latin american symposium presented by the american mosquito control association (amca) was held as part of the 80th annual meeting in seattle, wa, in february 2014. the principal objective, for the previous 23 symposia, was to promote participation in the amca by vector control specialists, public health workers, and academicians from latin america. this publication includes summaries of 26 presentations that were given orally in spanish or presented as posters by participants fr ...201425843096
an in vitro bioassay for the quantitative evaluation of mosquito repellents against stegomyia aegypti (=aedes aegypti) mosquitoes using a novel cocktail meal.to assess the efficacy of new insect repellents, an efficient and safe in vitro bioassay system using a multiple-membrane blood-feeding device and a cocktail meal was developed. the multiple-membrane blood-feeding device facilitates the identification of new insect repellents by the high-throughput screening of candidate chemicals. a cocktail meal was developed as a replacement for blood for feeding females of stegomyia aegypti (=aedes aegypti) (l.) (diptera: culicidae). the cocktail meal consis ...201525828787
cymbopogon citratus-synthesized gold nanoparticles boost the predation efficiency of copepod mesocyclops aspericornis against malaria and dengue mosquitoes.plant-borne compounds can be employed to synthesize mosquitocidal nanoparticles that are effective at low doses. however, how they affect the activity of mosquito predators in the aquatic environment is unknown. in this study, we synthesized gold nanoparticles (aun) using the leaf extract of cymbopogon citratus, which acted as a reducing and capping agent. aun were characterized by a variety of biophysical methods and sorted for size in order to confirm structural integrity. c. citratus extract ...201525819295
extract of bowdichia virgilioides and maackiain as larvicidal agent against aedes aegypti mosquito.the larvicidal activities of extracts of three hardwood species (hymenaea stigonorcapa, anadenanthera colubrina and bowdichia virgilioides) against 4th instar larvae of aedes aegypti were evaluated using who guidelines. extracts of h. stignocarpa and a. colubrina showed weak activity. the highest larvicidal effect was obtained with the cyclohexane extract of the heartwood of b. virgilioides, which caused 100% mortality at concentrations at 50 and 100 µg/ml. fraction toluene/etoac (8:2) from this ...201525819294
effect of salinity on the behavior of aedes aegypti populations from the coast and plateau of southeastern brazil.mosquito larvae can develop in fresh, salty and brackish water. the larvae of aedes aegypti develop in fresh water. however, in laboratory studies, tolerance of this species for oviposition and hatching in brackish water was observed. immature forms of ae. aegypti have also been found developing in brackish water in coastal areas. the purpose of the study was to compare the effects of salt on the biological cycle of ae. aegypti populations from coastal and plateau areas of southeastern brazil.201525815871
[assessment of the risk of expansion of the habitats of the mosquitoes aedes aegypti l. and aedes albopictus skus. in russia].based on climatic characteristics, the authors show that an invasive aedes albopictus species is likely to emerge in new areas of the russian federation.201525812400
an entomological review of invasive mosquitoes in europe.among the invasive mosquitoes registered all over the world, aedes species are particularly frequent and important. as several of them are potential vectors of disease, they present significant health concerns for 21st century europe. five species have established in mainland europe, with two (aedes albopictus and aedes japonicus) becoming widespread and two (ae. albopictus and aedes aegypti) implicated in disease transmission to humans in europe. the routes of importation and spread are often e ...201525804287
low efficacy of delthamethrin-treated net against singapore aedes aegypti is associated with kdr-type resistance.there has been a worldwide surge in the number and severity of dengue in the past decades. in singapore, relentless vector control efforts have been put in to control the disease since the 1960's. space spraying, fogging, chemical treatment and source reduction are some commonly used methodologies for controlling its vectors, particularly aedes aegypti. here, as we explored the use of a commercially available delthamethrin-treated net as an alternative strategy and the efficacy of the treated ne ...201525801264
an investigation on the diversity of mosquitocidal bacteria and its relationship with incidence of vector borne diseases.control of mosquitoes is the most important aspect of public health, as mosquitoes transmit many human diseases, including the fatal infection, japanese encephalitis. this paper addresses the isolation of new mosquitocidal bacteria from soil samples in the union territory of pondicherry, india, where, no clinical cases of vector borne infections have been reported. bacterial isolates from soil samples were screened for potential mosquitocidal strains and bioassays against mosquito vectors (culex ...201525801257
evaluation of sumithion l-40 against aedes aegypti (l.) and aedes albopictus skuse.space spraying of chemical insecticides is still an important mean of controlling aedes mosquitoes and dengue transmission. for this purpose, the bioefficacy of space-sprayed chemical insecticide should be evaluated from time to time. a simulation field trial was conducted outdoor in an open field and indoor in unoccupied flat units in kuala lumpur, to evaluate the adulticidal and larvicidal effects of sumithion l-40, a ulv formulation of fenitrothion. a thermal fogger with a discharge rate of 2 ...201525801256
feeding stage, species, body part and sex-specific activity of glutathione s-transferase in mosquito.in the present study, the feeding stage, body parts, development and sex specific activity of glutathione s-transferases (gsts) were observed in different mosquito species (aedes aegypti, culex quinquefasciatus, anopheles stephensi, an. culicifacies, an. annularis, an. subpictus, an. vagus). gst activity was assayed spectrophotometrically at 23°c, using a uv max microplate reader, to measure the rate of conjugation of gsh to cdnb. a significant species-specific difference in the activity of gst ...201525801255
surveillance of aedes albopictus skuse breeding preference in selected dengue outbreak localities, peninsular malaysia.entomological surveillance was conducted in order to determine the abundance and to evaluate any changes of biological vectors or ecology, especially in the dengue outbreak areas. the abundance and breeding preference of aedes albopictus and aedes aegypti were conducted in selected dengue outbreak localities in three states of peninsular malaysia namely selangor, federal territory of kuala lumpur, and penang island using ovitraps and larval survey method. it was determined that ae. albopictus wa ...201525801254
nectar protein content and attractiveness to aedes aegypti and culex pipiens in plants with nectar/insect associations.we chose five easily propagated garden plants previously shown to be attractive to mosquitoes, ants or other insects and tested them for attractiveness to culex pipiens and aedes aegypti. long term imbibition was tested by survival on each plant species. both mosquito species survived best on impatiens walleriana, the common garden impatiens, followed by asclepias curassavica, campsis radicans and passiflora edulis, which sponsored survival as well as the 10% sucrose control. immediate preferenc ...201525792420
possible association between recent migration and hospitalisation for dengue in an urban population: a prospective case-control study in northern vietnam.a prospective case-control study was conducted in urban districts in hanoi, northern vietnam to evaluate the effect of migration on the risk of hospitalisation for dengue in a vietnamese urban population. we enrolled laboratory-confirmed dengue patients aged ≥ 18 years who were hospitalised in local hospitals in november and december 2010. four neighbourhood-matched controls for each case were recruited within a week of hospitalisation. sociodemographic data were collected by interviews, and the ...201425776595
angelica sinensis (umbelliferae) with proven repellent properties against aedes aegypti, the primary dengue fever vector in thailand.botanical resources with great diversity in medicinal and aromatic plants are a rich and reliable source for finding insect repellents of plant origin, which are widely popular among today's consumers. although some herbal-based repellents have been proven comparable to or even better than synthetics, commercially available natural repellents generally tend to be expensive, with short-lived effectiveness. this critical flaw leads to ongoing research for new and effective repellents, which provid ...201525773182
aedes aegypti salivary gland extract ameliorates experimental inflammatory bowel disease.current therapies for inflammatory bowel disease (ibd) are not totally effective, resulting in persistent and recurrent disease for many patients. mosquito saliva contains immunomodulatory molecules and therein could represent a novel therapy for ibd. here, we demonstrated the therapeutic activity of salivary gland extract (sge) of aedes aegypti on dextran sulfate sodium (dss)-induced colitis. for this purpose, c57bl/6 male mice were exposed to 3% dss in drinking water and treated with sge at ea ...201525770821
first report of l1014f kdr mutation in culex quinquefasciatus in mexico.the l1014f mutation in the voltage-sodium channel gene has been associated with resistance to ddt and pyrethroids in various arthropod species including mosquitoes. we determined the frequency of the l1014f kdr mutation in 16 field populations of culex quinquefasciatus from northeastern mexico collected between 2008 and 2013. the l1014f was present in all populations analyzed with the lowest frequency (3.33%) corresponding to the population from monclova collected in 2012, and the highest freque ...201625765734
cytostatic and genotoxic effect of temephos in human lymphocytes and hepg2 cells.temephos is an organophosphorus pesticide that is used in control campaigns against aedes aegypti mosquitoes, which transmit dengue. in spite of the widespread use of temephos, few studies have examined its genotoxic potential. the aim of this study was to evaluate the cytotoxic, cytostatic and genotoxic effects of temephos in human lymphocytes and hepatoma cells (hepg2). the cytotoxicity was evaluated with simultaneous staining (fda/etbr). the cytostatic and genotoxic effects were evaluated usi ...201525746384
rnai knockdown of bmrab3 led to larva and pupa lethality in silkworm bombyx mori l.rab3 gtpases are known to play key a role in vesicular trafficking, and express highest in brain and endocrine tissues. in mammals, rab3 gtpases are paralogs unlike in insect. in this study, we cloned rab3 from the silk gland tissue of silkworm bombyx mori, and identified it as bmrab3. our in silico analysis indicated that bmrab3 is an isoform with a theoretical isoelectric point and molecular weight of 5.52 and 24.3 kda, respectively. further, bmrab3 showed the c-terminal hypervariability for g ...201525735242
effect of temperature on life history traits during immature development of aedes aegypti and culex quinquefasciatus (diptera: culicidae) from córdoba city, argentina.we investigated how ambient temperature under fluctuating conditions affects the larval-pupal immature traits of aedes aegypti and culex quinquefasciatus mosquitoes from córdoba city, argentina, and established each species development threshold and physiological time. based on life tables, three cohorts of each mosquito species were reared in the laboratory under small fluctuating temperatures conditions of 15.2±1.7°c, 17.9±1.6°c, 21.6±0.7°c and 25.3±0.4°c for ae. aegypti, and 16.6±1.7°c, 18.7± ...201525733491
identification of chemical constituents and larvicidal activity of essential oil from murraya exotica l. (rutaceae) against aedes aegypti, anopheles stephensi and culex quinquefasciatus (diptera: culicidae).this study was conducted to evaluate the phytochemical composition and larvicidal effect of leaf essential oil from murraya exotica against early fourth-instar larvae of aedes aegypti, anopheles stephensi and culex quinquefasciatus. gas chromatography (gc) and gas chromatography mass spectrometry (gc-ms) analyses revealed that the essential oil contained 27 components. the major chemical components identified were β-humulene (40.62%), benzyl benzoate (23.96%), β-caryophyllene (7.05%) and α-terpi ...201525697880
tackling the growing threat of dengue: phyllanthus niruri-mediated synthesis of silver nanoparticles and their mosquitocidal properties against the dengue vector aedes aegypti (diptera: culicidae).mosquitoes are vectors of devastating pathogens and parasites, causing millions of deaths every year. dengue is a mosquito-borne viral infection found in tropical and subtropical regions around the world. recently, transmission has strongly increased in urban and semiurban areas, becoming a major international public health concern. aedes aegypti (diptera: culicidae) is the primary vector of dengue. the use of synthetic insecticides to control aedes mosquitoes lead to high operational costs and ...201525669140
synthesis and characterization of silver nanoparticles using gmelina asiatica leaf extract against filariasis, dengue, and malaria vector mosquitoes.mosquitoes are blood-feeding insects and serve as the most important vectors for spreading human diseases such as malaria, yellow fever, dengue fever, and filariasis. the continued use of synthetic insecticides has resulted in resistance in mosquitoes. synthetic insecticides are toxic and affect the environment by contaminating soil, water, and air, and then natural products may be an alternative to synthetic insecticides because they are effective, biodegradable, eco-friendly, and safe to envir ...201525666372
immunological aspects of the immune response induced by mosquito allergens.allergies caused by mosquito bites may produce local or systemic reactions. the inhalation of mosquito allergens may also cause asthma and/or allergic rhinoconjunctivitis in sensitized individuals. the mechanisms implicated in the development of these immune responses involve ige antibodies, different subtypes of igg and proinflammatory cytokines as well as basophils, eosinophils and mast cells. several allergenic components have been identified in the saliva and bodies of mosquitoes and some of ...201425661054
evaluation of some aromatic plant extracts for mosquito larvicidal potential against culex quinquefasciatus, aedes aegypti, and anopheles stephensi.in the present investigation, larvicidal potential of hexane, choloroform, ethyl acetate, acetone, and methanol extracts of seven aromatic plants, viz., blumea mollis, chloroxylon swietenia, clausena anisata, feronia limnonia, lantana camera, plectranthus amboinicus, and tagetes erecta were screened against culex quinquefasciatus, aedes aegypti, and anopheles stephensi. the larval mortality was observed after 12 and 24 h of exposure period. the results revealed that all the extracts showed varie ...201525630696
dengue in brazil and colombia: a study of knowledge, attitudes, and practices.introduction this study was conducted in brazil and colombia,where dengue is endemic and vector control programs use chemical insecticides. methods we identified knowledge, attitudes, and practices about dengue and determined the infestation levels of aedes aegypti in one brazilian and four colombian communities. results the surveys show knowledge of the vector, but little knowledge about diagnosis, prognosis, and treatment. vector infestation indices show brazil to have good relative control, w ...201525626660
immature aedes mosquitoes colonize culex quinquefasciatus breeding sites in neighborhoods in the municipality of olinda, state of pernambuco.the present study shows the colonization of aedes mosquitoes in breeding sites specific for culex quinquefasciatus in neighborhoods in the municipality of olinda.201525626658
[control discourses and power relations of yellow fever: philadelphia in 1793].1793 yellow fever in philadelphia was the most severe epidemics in the late 18th century in the united states. more than 10% of the population in the city died and many people fled to other cities. the cause of yellow fever in the united states had close relationship with slaves and sugar in philadelphia. sugarcane plantation had needed many labors to produce sugar and lots of africans had to move to america as slaves. in this process, aëdes aegypti, the vector of yellow fever had migrated to am ...201425608507
mukia maderaspatana (cucurbitaceae) extract-mediated synthesis of silver nanoparticles to control culex quinquefasciatus and aedes aegypti (diptera: culicidae).mosquitoes and mosquito-borne diseases are prone to raise health and economic impacts. synthetic insecticide-based interventions are indeed in situations of epidemic outbreak and sudden increases of adult mosquitoes. nanoparticles are being used in many commercial applications and were found that aqueous silver ions can be reduced by an aqueous extract of plant parts to generate extremely stable silver nanoparticles in water. based on this, silver nanoparticles (snps) were synthesized using leaf ...201525601441
insecticidal and repellent activity of clausena dentata (rutaceae) plant extracts against aedes aegypti and culex quinquefasciatus mosquitoes (diptera: culicidae).mosquito control is facing a threat due to the emergence of resistance to synthetic insecticides. insecticides of botanical origin may serve as suitable alternative biocontrol agents. the present study is to evaluate adulticidal activity of clausena dentata plant extract against aedes aegypti and culex quinquefasciatus mosquitoes. the adult mortality was observed after 24 h of exposure. the highest mortality was found in acetone extracts against ae. aegypti and cx. quinquefasciatus with the lc50 ...201525573693
hptlc analysis of scoparia dulcis linn (scrophulariaceae) and its larvicidal potential against dengue vector aedes aegypti.this study evaluates the larvicidal activity of scoparia dulcis aqueous extract against dengue vector and determines its major chemical components. the extract was tested at various concentrations ranging from 0.1 to 2 mg/ml against aedes aegypti larvae. the extracts displayed significant larvicidal efficacy against ae. aegypt species after 24 h exposure revealing lc50 of 3.3835 (mg/ml) and lc90 of 5.7578 (mg/ml). finger printing profile carried out by camag automatic tlc sample applicator progr ...201525573588
aealrack1 expression and localization in response to stress in c6/36 ht mosquito cells.the receptor for activated c kinase 1 (rack1), a scaffold protein member of the tryptophan-aspartate (wd) repeat family, folds in a seven-bladed β-propeller structure that permits the association of proteins to form active complexes. mosquitoes of the genus aedes sp., are vectors of virus producing important diseases such as: dengue, chikungunya and yellow fever. based on the highly conserved gene sequence of aeaerack1 of the mosquito aedes aegypti we characterized the mrna and protein of the ho ...201525555378
larvicidal and repellent properties of streptomyces sp. vitjs4 crude extract against anopheles stephensi, aedes aegypti and culex quinquefasciatus (diptera: culicidae).the aim of the present study was to assess the larvicidal and repellent properties of marine streptomyces sp. vitjs4 crude extracts. the marine soil samples were collected from the puducherry coast, tamil nadu, india. the isolate streptomyces sp. vitjs4 was taxonomically characterized and identified. the ethyl acetate crude extract tested for larvicidal property showed 100% mortality for all the 3 species after 24 h exposure against the early fourth instar larvae of malarial vector--anopheles st ...201425546945
mosquito larvicidal potential of silver nanoparticles synthesized using chomelia asiatica (rubiaceae) against anopheles stephensi, aedes aegypti, and culex quinquefasciatus (diptera: culicidae).mosquitoes transmit serious human diseases, causing millions of deaths every year. mosquito control is to enhance the health and quality of life of county residents and visitors through the reduction of mosquito populations. mosquito control is a serious concern in developing countries like india due to the lack of general awareness, development of resistance, and socioeconomic reasons. today, nanotechnology is a promising research domain which has a wide ranging application in vector control pr ...201525544703
use of different coloured ovitraps in the surveillance of aedes mosquitoes in an arid-urban area of western rajasthan, india.in the absence of any commercially available dengue vaccine or antiviral therapy, controlling the primary mosquito vector, aedes aegypti, is currently the only means to prevent dengue outbreaks. ovitraps are being employed as a sensitive method for detecting the presence of ae. aegypti. size, colour, material, paddle and use of oviposition attractant have been reported as important factors influencing the efficacy of ovitraps.201425540965
dengue vectors prevalence and the related risk factors involved in the transmission of dengue in thiruvananthapuram district, kerala, south india.a longitudinal, entomological and virological study was conducted from 2007 to 2010 in four dengue fever affected areas of thiruvananthapuram district, kerala to understand the risk factors involved in the dengue transmission.201425540964
larvicidal activity of the leaf extracts of spondias mombin linn. (anacardiaceae) from various solvents against malarial, dengue and filarial vector mosquitoes (diptera: culicidae).aedes aegypti, anopheles gambiae and culex quinquefasciatus are vector mosquitoes of dengue, malaria, and filariasis, respectively. since no vaccine is available to treat these diseases, the control of the main mosquito vectors is essential. as conventional insecticides have limited success, plants may be alternative larvicidal agents, since they contain a rich source of bioactive chemicals. the aim of this study was to evaluate the larvicidal activity of methanol crude extract, hexane, dichloro ...201425540962
chemical composition and biological activity of four salvia essential oils and individual compounds against two species of mosquitoes.the chemical compositions of essential oils obtained from four species of genus salvia were analyzed by gas chromatography with a flame ionization detector (gc-fid) and gas chromatography-mass spectrometry (gc-ms). the main compounds identified from salvia species essential oils were as follows: 1,8-cineole (71.7%), α-pinene (5.1%), camphor (4.4%), and β-pinene (3.8%) in salvia apiana; borneol (17.4%), β-eudesmol (10.4%), bornyl acetate (5%), and guaiol (4.8%) in salvia elegans; bornyl acetate ( ...201525531412
effect of confertifolin from polygonum hydropiper l. against dengue vector mosquitoes aedes aegypti l.the essential oil from the leaves of polygonum hydropiper l. (polygonaceae) was tested against aedes aegypti l. the lc50 values were 190.72 and 234.37 ppm against second and fourth instar larvae of a. aegypti, respectively. confertifolin (6,6,9a-trimethy l-4,5,5a,6,7,8,9,9a-octahydronaphtho [1,2-c] furan-3 (1h)-one) was isolated from the essential oil of p. hydropiper leaves using silica gel column chromatography. the lc50 values were 2.90 and 2.96 ppm for second and fourth instar larvae of a. a ...201525523289
são paulo urban heat islands have a higher incidence of dengue than other urban areas.urban heat islands are characterized by high land surface temperature, low humidity, and poor vegetation, and considered to favor the transmission of the mosquito-borne dengue fever that is transmitted by the aedes aegypti mosquito. we analyzed the recorded dengue incidence in sao paulo city, brazil, in 2010-2011, in terms of multiple environmental and socioeconomic variables. geographical information systems, thermal remote sensing images, and census data were used to classify city areas accord ...201525523076
[evaluation of lethal ovitraps as a strategy for aedes aegypti control].dengue fever is a vector-borne viral disease of major public health importance. in the absence of a vaccine, control measures are primarily focused on reducing the mosquito vector. however, these strategies are very expensive, and the use of lethal ovitraps could contribute, in a practical manner, to the design of cost-effective strategies for vector control.201425504133
[ovitraps evaluation for surveillance and control of aedes aegypti in two urban settlements of urabá, antioquia].aedes (stegomyia) aegypti is the main vector of dengue. chemical insecticides have been used to control the mosquito and it has developed resistance. it is necessary to evaluate alternative strategies that are efficient, economical and easy to apply, such as ovitraps with bacillus thuringiesis israeliensis .201425504128
larvicidal activity of selected aloe species against aedes aegypti (diptera: culiciade).management of mosquito vectors by current classes of mosquitocides is relatively ineffective and necessitates prospecting for novel insecticides with different modes of action. larvicidal activities of 15 crude extracts from three geographically isolated aloe ngongensis (christian), aloe turkanensis (christian), and aloe fibrosa (lavranos & l.e.newton) (xanthorrhoeaceae) species (five each) were evaluated against aedes aegypti (linnaeus in hasselquist) (diptera: culiciade l.) yellow fever mosqui ...201425502038
bioactive compound synthesis of ag nanoparticles from leaves of melia azedarach and its control for mosquito larvae.larvicidal activity of synthesized ag nanoparticles using 2,7.bis[2-[diethylamino]-ethoxy]fluorence isolate from the melia azedarach leaves against aedes aegypti and culex quinquefasciatus. six fractions were collected and concentrated, fraction three showed a single spot on tlc which was found to be a pure compound. the structures were elucidated by analyses of uv, ms, and nmr spectral data. the maximum mortality was fluorence against a. aegypti and c. quinquefasciatus (lc50 = 7.94, lc90 = 23.8 ...201525496834
the use of modified mosquitoes in brazil for the control of aedes aegypti: methodological and ethical constraints. 201425493981
effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of aedes aegypti.the effects of piperonyl butoxide (pbo) on the toxicity of the organophosphate temephos (te) and the role of esterases in the resistance of aedes aegypti to this insecticide were evaluated.201425467258
impact of insecticide resistance on the field control of aedes aegypti in the state of são paulo.the need to control dengue transmission by means of insecticides has led to the development of resistance to most of the products used worldwide against mosquitoes. in the state of são paulo, the superintendência de controle de endemias (sucen) has annually monitored the susceptibility of aedes aegypti to insecticides since 1996; since 1999, surveys were conducted in collaboration with the national network of laboratories (morenaa network) and were coordinated by the ministry of health. in this ...201425467257
susceptibility to insecticides and resistance mechanisms in aedes aegypti from the colombian caribbean region.we determined the susceptibility to insecticides and the biochemical and molecular mechanisms involved in resistance in nine populations of aedes aegypti (l.) of the colombian caribbean region. bioassays were performed on larvae for susceptibility to temephos and on adults to the insecticides malathion, fenitrothion, pirimiphos-methyl, permethrin, deltamethrin, λ-cyhalothrin and cyfluthrin. the resistance ratio (rr) for each insecticide in the populations was determined, using the susceptible ro ...201425454522
a model for the development of aedes (stegomyia) aegypti as a function of the available food.we discuss the preimaginal development of the mosquito aedes aegypti from the point of view of the statistics of developmental times and the final body-size of the pupae and adults. we begin the discussion studying existing models in relation to published data for the mosquito. the data suggest a developmental process that is described by exponentially distributed random times. the existing data show as well that the idea of cohorts emerging synchronously is verified only in optimal situations c ...201525451964
chitin is a necessary component to maintain the barrier function of the peritrophic matrix in the insect midgut.in most insects, the peritrophic matrix (pm) partitions the midgut into different digestive compartments, and functions as a protective barrier against abrasive particles and microbial infections. in a previous study we demonstrated that certain pm proteins are essential in maintaining the pm's barrier function and establishing a gradient of pm permeability from the anterior to the posterior part of the midgut which facilitates digestion (agrawal et al., 2014). in this study, we focused on the e ...201525449129
efficacy of thai herbal essential oils as green repellent against mosquito vectors.repellency activity of thai essential oils derived from ylang ylang (cananga odorata (lamk.) hook.f. & thomson: annonaceae) and lemongrass (cymbopogon citratus (dc.) stapf: poaceae) were tested against two mosquito vectors, aedes aegypti (l.) and culex quinquefasciatus (say). there were compared with two chemical repellents (deet 20% w/w; sketolene shield(®) and ir3535, ethyl butylacetylaminopropionate 12.5% w/w; johnson's baby clear lotion anti-mosquito(®)). each herbal repellent was applied in ...201525438256
susceptibility of florida aedes aegypti and aedes albopictus to dengue viruses from puerto rico.locally acquired dengue cases in the continental u.s. are rare. however, outbreaks of dengue-1 during 2009, 2010, and 2013 in florida and dengue-1 and -2 in texas suggest vulnerability to transmission. travel and commerce between puerto rico and the u.s. mainland is common, which may pose a risk for traveler-imported dengue cases. mosquitoes were collected in florida and used to evaluate their susceptibility to dengue viruses (denv) from puerto rico. aedes aegypti and ae. albopictus were suscept ...201425424270
swarming mechanisms in the yellow fever mosquito: aggregation pheromones are involved in the mating behavior of aedes aegypti.mosquitoes of various species mate in swarms comprised of tens of thousands of flying males. in this study, we examined aedes aegypti swarming behavior and identified associated chemical cues. novel evidence is provided that ae. aegypti females aggregate by means of olfactory cues, such as aggregation pheromones. isolation of ae. aegypti aggregation pheromones was achieved by aeration of confined mosquitoes and collection of associated volatiles by glass filters. the collected volatiles were ide ...201425424264
behavioral responses of aedes aegypti, aedes albopictus, culex quinquefasciatus, and anopheles minimus against various synthetic and natural repellent compounds.the behavioral responses of colony populations of aedes aegypti, aedes albopictus, culex quinquefasciatus, and anopheles minimus to four essential oils (citronella, hairy basil, catnip, and vetiver), two standard repellents (deet and picaridin), and two synthetic pyrethroids (deltamethrin and permethrin) were conducted in the laboratory using an excito-repellency test system. results revealed that cx. quinquefasciatus and an. minimus exhibited much stronger behavioral responses to all test compo ...201425424262
synergistic repellent and irritant effect of combined essential oils on aedes aegypti (l.) mosquitoes.this study was designed to compare the behavioral responses of aedes aegypti to a single essential oil and to a mixture of two or three essential oils using an excito-repellency test chamber. mixtures were prepared from essential oils extracted from litsea cubeba (lc), litsea salicifolia (ls), and melaleuca leucadendron (ml). in general, the mixture of essential oils produced a much stronger escape response by ae. aegypti, regardless of the test conditions. no significant difference in escape re ...201425424258
a novel multiple membrane blood-feeding system for investigating and maintaining aedes aegypti and aedes albopictus mosquitoes.a novel multiple membrane blood-feeding system for mosquitoes has been developed for the study and routine maintenance of aedes aegypti l. and aedes albopictus skuse that require a meal of vertebrate blood to produce eggs. this blood-feeding system uses cattle collagen sausage-casing membrane to facilitate feeding. the efficiency of this blood-feeding system was compared to a live mice blood source. we observed that ae. aegypti that fed on pig whole blood had 89.7% (w/o atp) and 90.7% (w/ atp) b ...201425424255
the single water-surface sweep estimation method accurately estimates very low (n = 4) to low-moderate (n = 25-100) and high (n > 100) aedes aegypti (diptera: culicidae) pupae numbers in large water containers up to 13 times faster than the exhaustive sweep and total count method and without any sediment contamination.to confirm that a single water-surface sweep-net collection coupled with three calibration factors (2.6, 3.0 and 3.5 for 1/3, 2/3 and 3/3 water levels, respectively) (wscf) could accurately estimate very low to high aedes aegypti pupae numbers in water containers more rapidly than the exhaustive 5-sweep and total count (estc) method recommended by who.201525418067
evaluation of the role of atp-binding cassette transporters as a defence mechanism against temephos in populations of aedes aegypti.the role of atp-binding cassette (abc) transporters in the efflux of the insecticide, temephos, was assessed in the larvae of aedes aegypti. bioassays were conducted using mosquito populations that were either susceptible or resistant to temephos by exposure to insecticide alone or in combination with sublethal doses of the abc transporter inhibitor, verapamil (30, 35 and 40 μm). the best result in the series was obtained with the addition of verapamil (40 μm), which led to a 2x increase in the ...201425411004
chikungunya.chikungunya is a rapidly emerging infectious disease caused by a virus of the genus alphavirus, family togaviridae. most commonly, patients have an acute onset of fever with often debilitating symmetric joint discomfort that can relapse months after the initial infection. this infection is typically transmitted by the bite of an infected aedes aegypti or aedes albopictus mosquito, vectors that also transmit dengue and yellow fever. special operations forces medical providers should be aware of t ...201425399382
the larvicidal activity of agave sisalana against l4 larvae of aedes aegypti is mediated by internal necrosis and inhibition of nitric oxide production.dengue is a viral disease that affects about 50 million people per year around the world. the aim of this study was to investigate the larvicidal activity of agave sisalana crude extract in order to develop a new insecticide against aedes aegypti. in larvicidal activity assays, fourth-stage ae. aegypti larvae were exposed to different concentrations of a. sisalana crude extract for 3, 6, 12, and 24 h for determining the lc50. next, we explored its cytotoxic activity by flow cytometry. furthermor ...201525395257
detection and isolation of the α-proteobacterium asaia in culex mosquitoes.investigations of microbiota within mosquitoes continue to widen the spectrum of possible symbiont-based applications against vector-borne diseases. in this context, α-proteobacteria of the genus asaia (rhodospirillales: acetobacteraceae) are emerging as possible endosymbiotic candidates, particularly in paratransgenic approaches aimed at interrupting pathogen transmission. previous studies have shown that asaia spp. distribution among anopheles gambiae and anopheles stephensi (diptera: culicida ...201425387864
oviposition deterring and oviciding potentials of ipomoea cairica l. leaf extract against dengue vectors.bioprospecting of plant-based insecticides for vector control has become an area of interest within the last two decades. due to drawbacks of chemical insecticides, phytochemicals of plant origin with mosquito control potential are being utilized as alternative sources in integrated vector control. in this regard, the present study aimed to investigate oviposition deterring and oviciding potentials of ipomoea cairica (l.) (family: convolvulaceae) crude leaf extract against dengue vectors, aedes ...201425382472
surveillance and resistance status of aedes population in two suburban residential areas in kampar town, perak, malaysia.in this study, 13 weeks (october to december 2012) of ovitrap surveillance was conducted in two suburban residential areas in kampar town, perak. a total of 17,310 aedes mosquitoes were found in taman kampar jaya, whereas taman juloong recorded a higher number at 19,042. less than 1% of these were identified as aedes aegypti, with the remaining confirmed as aedes albopictus. the female ae. albopictus were subsequently subjected to who standard diagnostic test kits against two pyrethroids (0.05% ...201425382470
larvicidal efficacy of different plant parts of railway creeper, ipomoea cairica extract against dengue vector mosquitoes, aedes albopictus (diptera: culicidae) and aedes aegypti (diptera: culicidae).natural insecticides from plant origin against mosquito vectors have been the main concern for research due to their high level of eco-safety. control of mosquitoes in their larval stages are an ideal method since aedes larvae are aquatic, thus it is easier to deal with them in this habitat. the present study was specifically conducted to explore the larvicidal efficacy of different plant parts of ipomoea cairica (l.) or railway creeper crude extract obtained using two different solvents; methan ...201425368088
dengue outbreaks in divinopolis, south-eastern brazil and the geographic and climatic distribution of aedes albopictus and aedes aegypti in 2011-2012.to entomologically monitor aedes spp. and correlate the presence of these vectors with the recent epidemic of dengue in divinopolis, minas gerais state, brazil.201525328988
adulticidal and smoke toxicity of cipadessa baccifera (roth) plant extracts against anopheles stephensi, aedes aegypti, and culex quinquefasciatus.mosquito vectors are responsible for the transmission of parasitic and viral infections, including loss in commercial and labor outputs, particularly in developing countries with tropical and subtropical climates. the aim of the present study is to evaluate the adulticidal and smoke toxicity of cipadessa baccifera (roth) against three important mosquitoes vectors, anopheles stephensi, aedes aegypti, and culex quinquefasciatus (diptera: culicidae). adult mortality was observed after 24-h recovery ...201525320044
screening of rubiaceae and apocynaceae extracts for mosquito larvicidal potential.rubiaceae and apocynaceae families are well known for the expression of cyclotides having insecticidal properties. leaves and flowers extracts of plants from the families rubiaceae (ixora coccinea) and apocynaceae (allamanda violacea) were evaluated for mosquito larvicidal effect against early ivth instars of aedes aegypti and anopheles stephensi. two forms of plant extracts, one untreated and the other treated with heat and proteolytic enzyme were used for assay. after primary assay, the extrac ...201525317964
effects of croton rhamnifolioides essential oil on aedes aegypti oviposition, larval toxicity and trypsin activity.although numerous reports are available concerning the larvicidal potential of essential oils, very few investigations have focused on their mechanisms of action. in the present study, we have investigated the chemical composition of the leaf oil of croton rhamnifolioides during storage and its effects on oviposition and survival of larvae of the dengue fever mosquito aedes aegypti. in addition, we have established a possible mechanism of action for the larvicidal activity of the essential oil. ...201425317582
effective surveillance of vector dynamics of aedes aegypti in a hospital setting in cuiabá, mato grosso, brazil.hospitals are important for vector control of endemic diseases.201425313617
social sustainability of mesocyclops biological control for dengue in south vietnam.copepod mesocyclops as biological control agents for dengue was previously proven to be effective and sustainable in the northern and central provinces of vietnam. we aim to study social sustainability of mesocyclops intervention in south vietnam. both quantitative and qualitative approaches were used. an entomological survey was carried out in 100 random households of chanh an commune, vinh long province. aedes larval indices and mesocyclops prevalence were compared with historical pre- and pos ...201525312335
distribution of yellow fever vectors in northwestern and western provinces, zambia.to determine the distribution of yellow fever (yf) vectors species in northwestern and western of zambia, which sampled mosquitoes inside and outside houses in rural, urban, peri-urban and forest areas.201425312199
larvicidal activity of persea americana mill. against aedes aegypti.to evaluate the toxicity of the ethanol and hexane extracts of the different parts of persea americana mill. (p. americana) toward third and fourth instars larvae of aedes aegypti (ae. aegypti) and to characterize the ethanol extract by qualitative phytochemical analysis.201425312114
evaluation of plant-mediated synthesized silver nanoparticles against vector mosquitoes.diseases transmitted by blood-feeding mosquitoes, such as dengue fever, dengue hemorrhagic fever, japanese encephalitis, malaria, and filariasis, are increasing in prevalence, particularly in tropical and subtropical zones. to control mosquitoes and mosquito-borne diseases, which have worldwide health and economic impacts, synthetic insecticide-based interventions are still necessary, particularly in situations of epidemic outbreak and sudden increases of adult mosquitoes. green nanoparticle syn ...201425300419
subacute toxicity assessment of diflubenzuron, an insect growth regulator, in adult male rats.diflubenzuron (dfb), an insecticide and acaricide insect growth regulator, can be used in agriculture against insect predators and in public health programs, to control insects and vectors, mainly aedes aegypti larvae. due to the lack of toxicological assessments of this compound, the objective of the present study was to evaluate the toxicological effects of subacute exposure to the dfb insecticide in adult male rats. adult male rats were exposed (gavage) to 0, 2, 4, or 8 mg/kg of dfb for 28 da ...201625266294
larvicidal and phytochemical properties of callistemon rigidus r. br. (myrtaceae) leaf solvent extracts against three vector mosquitoes.due to ever-growing insecticide resistance in mosquito vectors and environmental contamination by synthetic insecticides, plants may be a source of alternative agents for mosquito control. therefore, the present investigation involved the determination of larvicidal and phytochemical properties of callistemon rigidus leaf extracts against anopheles gambiae, aedes aegypti and culex quinquefasciatus.201425253215
how dengue vector aedes albopictus (diptera: culicidae) survive during the dry season in dhaka city, bangladesh?in 2000, a dengue outbreak occurred in bangladesh that included dhaka city. both dengue vectors, aedes aegypti and ae. albopictus are present in bangladesh. aedes aegypti mosquitoes mainly breed in and around houses and ae. albopictus is an outside breeder. there are many old trees throughout dhaka city in different parks, streets and the university campus which may have holes that can contribute as potential breeding habitat for the dengue vector. therefore, a survey was conducted to investigat ...201425253210
multiobjective genetic algorithm applied to dengue control.dengue fever is an infectious disease caused by a virus of the flaviridae family and transmitted to the person by a mosquito of the genus aedes aegypti. this disease has been a global public health problem because a single mosquito can infect up to 300 people and between 50 and 100 million people are infected annually on all continents. thus, dengue fever is currently a subject of research, whether in the search for vaccines and treatments for the disease or efficient and economical forms of mos ...201425230238
discrepancies between aedes aegypti identification in the field and in the laboratory after collection with a sticky trap.currently, sticky traps are regularly employed to assist in the surveillance of aedes aegypti infestation. we tested two alternative procedures for specimen identification performed by local health agents: directly in the field, as recommended by certain manufacturers, or after transportation to the laboratory. a total of 384 sticky traps (mosquitrap) were monitored monthly during one year in four geographically representative brazilian municipalities. when the same samples were inspected in the ...201425230130
synthesis of silver nanoparticles from azadirachta indica--a most effective method for mosquito control.mosquitoes transmit major communicable diseases such as dengue, malaria, filariasis, japanese encephalitis, chikungunya, and so on. vector control is important in epidemic disease situations as there is an urgent need to develop new and improved mosquito control methods that are economical and effective yet safe for non-targeted organisms. in the present study, silver nanoparticles (agnps) were synthesized from the aqueous leaf extract of neem plant (azadirachta indica), and their effects on mos ...201525226837
dengue in children.dengue is a mosquito-borne viral disease of expanding geographical range and increasing incidence. the vast majority of dengue cases are children less than 15 years of age. dengue causes a spectrum of illness from mild fever to severe disease with plasma leakage and shock. infants and children with secondary heterologous dengue infections are most at risk for severe dengue disease. laboratory diagnosis of dengue can be established within five days of disease onset by direct detection of viral co ...201425225163
studies on repellent activity of seed oils alone and in combination on mosquito, aedes aegypti.the study was undertaken to investigate the relative repellency of pongamia pinnata and azadirachta indica seed oils on vector mosquito, aedes aegypti under laboratory conditions. the repellents were formulated into 3 groups: seed oils, their mixture and combination of seed oils with three carrier oils viz. olive, mustard and coconut oil. different formulations of each oil were tested at the concentrations of 1% and 5% on human baits. efficiency was assessed, based on the total protection time; ...201425204067
[dengue fever in portuguese speaking countries: which epidemiological links may we set?].the recent occurrence of a number of outbreaks of dengue in portuguese speaking countries with no previous disease, aroused curiosity about the competing factors for the phenomenon and a need for better knowledge of the pathology. we review the dengue-related situation in portuguese speaking countries, linking the various outbreaks and trying to contribute to a better understanding of the phenomenon.201425203960
chikungunya fever. rheumatic manifestations of an emerging disease in europe.chikungunya fever is a viral disease caused by an alphavirus belonging to the togaviridae family, transmitted by several species of aedes mosquitoes: aedes aegypti and aedes albopictus (a. albopictus). it is endemic in africa and asia with recurrent outbreaks. it is an emerging disease and cases in europe transmitted by a. albopictus have been established in mediterranean areas. the first autochthonous cases detected on the caribbean islands suppose a serious threat of spreading disease to ameri ...201625192946
dengue and dengue vectors in the who european region: past, present, and scenarios for the future.after 55 years of absence, dengue has re-emerged in the who european region both as locally transmitted sporadic cases and as an outbreak in madeira, driven by the introduction of people infected with the virus and the invasion of the vector mosquito species aedes aegypti and aedes albopictus. models predict a further spread of a albopictus, particularly under climate change conditions. dengue transmission models suggest a low risk in europe, but these models too rarely include transmission by a ...201425172160
invasion of wolbachia at the residential block level is associated with local abundance of stegomyia aegypti, yellow fever mosquito, populations and property attributes.wolbachia can suppress dengue and control mosquito populations and this depends on the successful invasion of wolbachia-infected mosquitoes into local populations. ovitrap data collected during the recent invasion of wmel-infected stegomyia aegypti (diptera: culicidae) (linnaeus) into gordonvale near cairns, australia, were used to identify variables that help predict the success of localized invasion. based on the variance in wolbachia frequencies across gordonvale as well as at another release ...201425171611
estimation of aedes aegypti (diptera: culicidae) population size and adult male survival in an urban area in panama.traditional mosquito control strategies rely heavily on the use of chemical insecticides. however, concerns about the efficiency of traditional control methods, environmental impact and emerging pesticide resistance have highlighted the necessity for developing innovative tools for mosquito control. some novel strategies, including release of insects carrying a dominant lethal gene (ridl®), rely on the sustained release of modified male mosquitoes and therefore benefit from a thorough understand ...201425165979
insecticidal, repellent and fungicidal properties of novel trifluoromethylphenyl amides.twenty trifluoromethylphenyl amides were synthesized and evaluated as fungicides and as mosquito toxicants and repellents. against aedes aegypti larvae, n-(2,6-dichloro-4-(trifluoromethyl)phenyl)-3,5-dinitrobenzamide (1e) was the most toxic compound (24 h lc50 1940 nm), while against adults n-(2,6-dichloro-4-(trifluoromethyl)phenyl)-2,2,2-trifluoroacetamide (1c) was most active (24 h ld50 19.182 nm, 0.5 μl/insect). however, the 24 h lc50 and ld50 values of fipronil against ae. aegypti larvae and ...201325149248
insecticide resistance and, efficacy of space spraying and larviciding in the control of dengue vectors aedes aegypti and aedes albopictus in sri lanka.unprecedented incidence of dengue has been recorded in sri lanka in recent times. source reduction and use of insecticides in space spraying/fogging and larviciding, are the primary means of controlling the vector mosquitoes aedes aegypti and ae. albopictus in the island nation. a study was carried out to understand insecticide cross-resistance spectra and mechanisms of insecticide resistance of both these vectors from six administrative districts, i.e. kandy, kurunegala, puttalam, gampaha, ratn ...201325149242
adulticidal properties of synthesized silver nanoparticles using leaf extracts of feronia elephantum (rutaceae) against filariasis, malaria, and dengue vector mosquitoes.mosquito-borne diseases with an economic impact create loss in commercial and labor outputs, particularly in countries with tropical and subtropical climates. mosquito control is facing a threat because of the emergence of resistance to synthetic insecticides. extracts from plants may be alternative sources of mosquito control agents because they constitute a rich source of bioactive compounds that are biodegradable into nontoxic products and potentially suitable for use to control mosquitoes. i ...201425146645
larvicidal efficacy screening of anacardaciae crude extracts on the dengue hemorrhagic vector, aedes aegypti.vector-borne diseases are still rife because of the re-emergence of diseases transmitted by mosquitoes. the objective of this paper is to evaluate the larvicidal efficacy of crude leaf extract of mangifera indica, gluta renghas, and melanochyla fasciculiflora against vector of dengue hemorrhagic fever, aedes aegypti. these plant species are endemic species and widely distributed in malaysian forests. leaves of ma. indica, g. renghas and m. fascculiflora were collected from teluk bahang national ...201425134898
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