| complete genome sequence of the polychlorinated biphenyl-degrading bacterium pseudomonas putida kf715 (nbrc 110667) isolated from biphenyl-contaminated soil. | pseudomonas putida kf715 (nbrc 110667) utilizes biphenyl as a sole source of carbon and degrades polychlorinated biphenyls (pcbs). here, we report a complete genome sequence of the kf715 strain, which comprises a circular chromosome and four plasmids. biphenyl catabolic genes were located on the largest plasmid, pkf715a. | 2017 | 28209826 |
| quantifying bacterial attachment and detachment using leaching solutions of various ionic strengths after bacterial pulse. | in this study, we quantified the attachment and detachment of bacteria during transport in order to elucidate the contributions of reversible attachment on bacterial breakthrough curves. the first set of breakthrough experiment was performed for a laboratory sand column using leaching solutions of deionized water and mineral salt medium (msm) of 200 mm with reference to kcl solution by employing pseudomonas putida as a model bacterium. in the second set of experiment, the ionic strengths of leac ... | 2017 | 28197984 |
| carbon source-dependent inducible metabolism of veratryl alcohol and ferulic acid in pseudomonas putida csv86. | pseudomonas putida csv86 degrades lignin-derived metabolic intermediates, viz, veratryl alcohol, ferulic acid, vanillin, and vanillic acid, as the sole sources of carbon and energy. strain csv86 also degraded lignin sulfonate. cell respiration, enzyme activity, biotransformation, and high-pressure liquid chromatography (hplc) analyses suggest that veratryl alcohol and ferulic acid are metabolized to vanillic acid by two distinct carbon source-dependent inducible pathways. vanillic acid was furth ... | 2017 | 28188206 |
| pathogen cross-transmission via building sanitary plumbing systems in a full scale pilot test-rig. | the who consensus document on the epidemiology of the sars epidemic in 2003, included a report on a concentrated outbreak in one hong kong housing block which was considered a 'super-spreading event'. the who report conjectured that the sanitary plumbing system was one transmission route for the virus. empty u-traps allowed the aerosolised virus to enter households from the sewerage system. no biological evidence was presented. this research reports evidence that pathogens can be aerosolised and ... | 2017 | 28187135 |
| acc deaminase-containing plant growth-promoting rhizobacteria protect papaver somniferum from downy mildew. | the aim of this study was to determine whether acc (1-aminocyclopropane-1-carboxylic acid) deaminase-containing bacterial treatments could enhance the tolerance of poppy (papaver somniferum l.) plants against biotic stress of downy mildew caused by peronospora sp. | 2017 | 28186676 |
| medium chain length polyhydroxyalkanoates biosynthesis in pseudomonas putida mt-2 is enhanced by co-metabolism of glycerol/octanoate or fatty acids mixtures. | the synthesis of medium chain length polyhydroxyalkanoates (mcl-phas) by pseudomonas putida mt-2 was investigated under nitrogen-rich then deficient conditions with glycerol/octanoate or long-chain fatty acids (lcfas) as carbon sources. when mixed, glycerol and octanoate were co-assimilated regardless of nitrogen availability but provided that glycerol uptake has been already triggered under non-limiting nutrient conditions. this concomitant consumption allowed to enhance mcl-phas accumulation ( ... | 2017 | 28174083 |
| a two-column flash chromatography approach to pyoverdin production from pseudomonas putida gb1. | our knowledge of the biological and environmental reactivity of siderophores is limited by the difficulty and cost of obtaining reasonable quantities by purification or synthesis. in this note, we describe a modified procedure for the low-cost, mg-scale purification of pyoverdin-type siderophores using a dual-flash chromatography (reverse-phase absorption and size exclusion) approach. | 2017 | 28161587 |
| itraq-based quantitative proteomic analysis of the global response to 17β-estradiol in estrogen-degradation strain pseudomonas putida sjte-1. | microorganism degradation is efficient to remove the steroid hormones like 17β-estradiol (e2); but their degradation mechanism and metabolic network to these chemicals are still not very clear. here the global responses of the estrogen-degradation strain pseudomonas putida sjte-1 to 17β-estradiol and glucose were analyzed and compared using the itraq (isobaric tags for relative and absolute quantization) strategy combined with lc-ms/ms (liquid chromatography-tandem mass spectrometry). 78 protein ... | 2017 | 28155874 |
| structure and biochemistry of phenylacetaldehyde dehydrogenase from the pseudomonas putida s12 styrene catabolic pathway. | phenylacetaldehyde dehydrogenase catalyzes the nad(+)-dependent oxidation of phenylactealdehyde to phenylacetic acid in the styrene catabolic and detoxification pathway of pseudomonas putida (s12). here we report the structure and mechanistic properties of the n-terminally histidine-tagged enzyme, npadh. the 2.83 å x-ray crystal structure is similar in fold to sheep liver cytosolic aldehyde dehydrogenase (aldh1), but has unique set of intersubunit interactions and active site tunnel for substrat ... | 2017 | 28153386 |
| rapid proteomics to prospect and validate novel bacterial metabolism induced by environmental burden. | understanding the pathophysiology of genes and enzymes involved in caffeine metabolism can have extracurricular benefits, such as providing distinct methylxanthines as intermediates for pharmaceutical synthesis, and also improve environmental waste remediation. the strains pseudomonas putida cbb5 and ces may provide insights into these applications because they may both be induced to degrade caffeine, yet the latter thrives in concentrations >8.0gl(-1); threefold higher than any other bacteria. ... | 2017 | 28137573 |
| proteins with chads (conserved histidine α-helical domains) are attached to polyphosphate granules in vivo and constitute a novel family of polyphosphate-associated proteins (phosins). | on the basis of bioinformatic evidence, we suspected that proteins with a cyth (cyab thiamine triphosphatase) domain and/or a chad (conserved histidine α-helical domain) motif might represent polyphosphate (polyp) granule-associated proteins. we found no evidence of polyp targeting by proteins with cyth domains. in contrast, two chad motif-containing proteins from ralstonia eutropha h16 (a0104 and b1017) that were expressed as fusions with enhanced yellow fluorescent protein (eyfp) colocalized w ... | 2017 | 28130300 |
| global transcriptional responses to osmotic, oxidative, and imipenem stress conditions in pseudomonas putida. | bacteria cope with and adapt to stress by modulating gene expression in response to specific environmental cues. in this study, the transcriptional response of pseudomonas putida kt2440 to osmotic, oxidative, and imipenem stress conditions at two time points was investigated via identification of differentially expressed mrnas and small rnas (srnas). a total of 440 srna transcripts were detected, of which 10% correspond to previously annotated srnas, 40% to novel intergenic transcripts, and 50% ... | 2017 | 28130298 |
| microbial biotransformation of five pyrrolidinophenone-type psychoactive substances in wastewater and a wastewater isolated pseudomonas putida strain. | wastewater-based epidemiology (wbe) employs the analysis of wastewater to detect and quantify drug use and discharge within a community. in this work, transformation products (tp) by microbes in the environment were identified after incubations in wastewater and an isolated microbial strain. the microbial strain was isolated from an enrichment culture of wastewater supplemented with 3,4-methylenedioxy-pyrovalerone, and identified by matrix assisted laser desorption - time of flight mass spectrom ... | 2017 | 28092435 |
| pathway-consensus approach to metabolic network reconstruction for pseudomonas putida kt2440 by systematic comparison of published models. | over 100 genome-scale metabolic networks (gsmns) have been published in recent years and widely used for phenotype prediction and pathway design. however, gsmns for a specific organism reconstructed by different research groups usually produce inconsistent simulation results, which makes it difficult to use the gsmns for precise optimal pathway design. therefore, it is necessary to compare and identify the discrepancies among networks and build a consensus metabolic network for an organism. here ... | 2017 | 28085902 |
| the pseudomonas putida t6ss is a plant warden against phytopathogens. | bacterial type vi secretion systems (t6sss) are molecular weapons designed to deliver toxic effectors into prey cells. these nanomachines have an important role in inter-bacterial competition and provide advantages to t6ss active strains in polymicrobial environments. here we analyze the genome of the biocontrol agent pseudomonas putida kt2440 and identify three t6ss gene clusters (k1-, k2- and k3-t6ss). besides, 10 t6ss effector-immunity pairs were found, including putative nucleases and pore-f ... | 2017 | 28045455 |
| active antibacterial and antifouling surface coating via a facile one-step enzymatic cross-linking. | prevention of microbial contamination of surfaces is one of the biggest challenges for biomedical applications. establishing a stable, easily produced, highly antibacterial surface coating offers an efficient solution but remains a technical difficulty. here, we report on a new approach to create an in situ hydrogel film-coating on glass surfaces made by enzymatic cross-linking under physiological conditions. the cross-linking is catalyzed by horseradish peroxidase (hrp)/glucose oxidase (god)-co ... | 2017 | 28005352 |
| plant defence induced by pgpr against spodoptera litura in tomato (solanum lycopersicum l.). | the research conducted including its rationale: spodoptera litura is the major pest of tomato causing significant reduction in tomato yield. application of plant growth promoting rhizobacteria(pgpr) prevent use of chemical fertilizer and synthetic pesticides through enhancement of plant growth and yield and induction of systemic resistance. present investigation is an attempt to evaluate the role of pgpr, pseudomonas putida and rothia sp. on the physiology and yield of tomato fruit infested with ... | 2017 | 28004873 |
| phylogenetic analysis reveals the taxonomically diverse distribution of the pseudomonas putida group. | pseudomonas putida is well-known for degradation activities for a variety of compounds and its infections have been reported. thus, p. putida includes both clinical and nonclinical isolates. to date, no reports have examined the phylogenetic relationship between clinical and nonclinical isolates of the p. putida group. in this study, fifty-nine strains of p. putida group containing twenty-six clinical, and thirty-three nonclinical, isolates, were subjected to phylogenetic and taxonomic analyses ... | 2017 | 27989998 |
| structural analysis provides mechanistic insight into nicotine oxidoreductase from pseudomonas putida. | the first structure of nicotine oxidoreductase (nica2) was determined by x-ray crystallography. pseudomonas putida has evolved nicotine-degrading activity to provide a source of carbon and nitrogen. the structure establishes nica2 as a member of the monoamine oxidase family. residues 1-50 are disordered and may play a role in localization. the nicotine-binding site proximal to the isoalloxazine ring of flavin shows an unusual composition of the classical aromatic cage (w427 and n462). the active ... | 2016 | 27933790 |
| saponaria officinalis l. extract: surface active properties and impact on environmental bacterial strains. | plant-derived surfactants are characterised by low toxicity, high biodegradability and environmental compatibility. they therefore have many applications; for instance, they can be used in bioremediation to accelerate biodegradation processes, especially of hydrophobic pollutants. this paper analyses the properties of an extract from saponaria officinalis l. containing saponins and its impact on bacterial strains isolated from soil, as well as its potential for application in hydrocarbon bioreme ... | 2017 | 27918965 |
| functional components of the bacterial czccba efflux system reduce cadmium uptake and accumulation in transgenic tobacco plants. | cadmium (cd) is a toxic trace element released into the environment by industrial and agricultural practices, threatening the health of plants and contaminating the food/feed chain. biotechnology can be used to develop plant varieties with a higher capacity for cd accumulation (for use in phytoremediation programs) or a lower capacity for cd accumulation (to reduce cd levels in food and feed). here we generated transgenic tobacco plants expressing components of the pseudomonas putida czccba effl ... | 2017 | 27902938 |
| methods for the isolation of genes encoding novel pha metabolism enzymes from complex microbial communities. | development of different phas as alternatives to petrochemically derived plastics can be facilitated by mining metagenomic libraries for diverse pha cycle genes that might be useful for synthesis of bio-plastics. the specific phenotypes associated with mutations of the pha synthesis pathway genes in sinorhizobium meliloti and pseudomonas putida, allows the use of powerful selection and screening tools to identify complementing novel pha synthesis genes. identification of novel genes through thei ... | 2017 | 27900694 |
| a high-throughput microtiter plate assay for the discovery of active and enantioselective amino alcohol-specific transaminases. | chiral vicinal amino alcohols are important chiral building blocks and intermediates in the pharmaceutical industry. the transaminase (tam) catalyzed kinetic resolution of racemic amino alcohols provides a straightforward approach to access these important compounds. this study describes the development of a novel microtiter plate assay to screen vicinal amino alcohol-specific tams using a tetrazolium red-based colorimetric assay to monitor the rate of α-hydroxy ketone formation at 510 nm. this ... | 2017 | 27899283 |
| application of the freeze-dried bioluminescent bioreporter pseudomonas putida mt-2 kg1206 to the biomonitoring of groundwater samples from monitoring wells near gasoline leakage sites. | this study examined the applicability of a freeze-dried bioluminescent bioreporter, pseudomonas putida mt-2 kg1206 (called kg1206), to the biomonitoring of groundwater samples. samples were collected from the monitoring wells of gas station tanks or old pipeline leakage sites in korea. in general, the freeze-dried strain in the presence of pure inducer chemicals showed low bioluminescence activity and a different activity order compared with that of the subcultured strain. the effects of kno3 as ... | 2017 | 27853856 |
| a broad mercury resistant strain of pseudomonas putida secretes pyoverdine under limited iron conditions and high mercury concentrations. | the pseudomonas putida fb1, known as a broad-spectrum mercury resistant strain, becomes yellow-green due to the secretion of pyoverdine (pvds) under limited iron conditions and high mercury concentrations. different modified nelson's media were obtained by adding mercury, iron, and the complexing agent nitrilotriacetic acid to demonstrate that the strain produces only the highest concentrations of pvds due to the induction with 25 µm hg(2+). an amount of 250 mg pvds was purified from the superna ... | 2016 | 27848043 |
| biofilm removal using carbon dioxide aerosols without nitrogen purge. | biofilms can cause serious concerns in many applications. not only can they cause economic losses, but they can also present a public health hazard. therefore, it is highly desirable to remove biofilms from surfaces. many studies on co2 aerosol cleaning have employed nitrogen purges to increase biofilm removal efficiency by reducing the moisture condensation generated during the cleaning. however, in this study, periodic jets of co2 aerosols without nitrogen purges were used to remove pseudomona ... | 2016 | 27842343 |
| influence of ph on dynamics of microbial enhanced oil recovery processes using biosurfactant producing pseudomonas putida: mathematical modelling and numerical simulation. | in present work, the influence of reservoir ph conditions on dynamics of microbial enhanced oil recovery (meor) processes using pseudomonas putida was analysed numerically from the developed mathematical model for meor processes. further, a new strategy to improve the meor performance has also been proposed. it is concluded from present study that by reversing the reservoir ph from highly acidic to low alkaline condition (ph 5-8), flow and mobility of displaced oil, displacement efficiency, and ... | 2017 | 27836230 |
| elimination of persistent emerging micropollutants in a suspended-bed photocatalytic reactor: influence of operating conditions and combination with aerobic biological treatment. | a larger, lab-scale photocatalytic suspended-bed reactor using tio2 sol-gel-coated expanded clay granules as a bed material was evaluated for oxidative removal of the persistent pharmaceuticals doxycycline, prednisolone, amoxicillin, and sulfamethizole, as well as their mixture, in ppm concentrations. the photocatalytic degradation potential of drug molecules increases as their adsorption affinity increases towards tio2-containing coatings. the performance of the photocatalytic reactor in the re ... | 2016 | 27830220 |
| development and evaluation of an experimental protocol for 3-d visualization and characterization of the structure of bacterial biofilms in porous media using laboratory x-ray tomography. | the development of a reliable model allowing accurate predictions of biofilm growth in porous media relies on a good knowledge of the temporal evolution of biofilm structure within the porous network. since little is known about the real 3-d structure of biofilms in porous media, this work was aimed at developing a new experimental protocol to visualize the 3-d microstructure of the inside of a porous medium using laboratory x-ray microtomography. a reliable and reproducible methodology is propo ... | 2016 | 27827532 |
| ecological risk assessment of pharmaceuticals in the receiving environment of pharmaceutical wastewater in pakistan. | the pharmaceutical industry of pakistan is growing with an annual growth rate of 10%. besides this growth, this industry is not complying with environmental standards, and discharging its effluent into domestic wastewater network. only limited information is available about the occurrence of pharmaceutical compounds (pcs) in the environmental matrices of pakistan that has motivated us to aim at the occurrence and ecological risk assessment of 11 pcs of different therapeutic classes in the wastew ... | 2017 | 27810578 |
| responses of pseudomonas putida to zinc excess determined at the proteome level: pathways dependent and independent of colrs. | zinc is an important micronutrient for bacteria, but its excess is toxic. recently, the colrs two-component system was shown to detect and respond to zinc excess and to contribute to zinc tolerance of pseudomonas putida. here, we applied a label-free whole-cell proteome analysis to compare the zinc-induced responses of p. putida and colr knockout. we identified dozens of proteins that responded to zinc in a colr-independent manner, among others, known metal efflux systems czccba1, czccba2, cada2 ... | 2016 | 27797207 |
| biochemical and genetic bases of indole-3-acetic acid (auxin phytohormone) degradation by the plant-growth-promoting rhizobacterium paraburkholderia phytofirmans psjn. | several bacteria use the plant hormone indole-3-acetic acid (iaa) as a sole carbon and energy source. a cluster of genes (named iac) encoding iaa degradation has been reported in pseudomonas putida 1290, but the functions of these genes are not completely understood. the plant-growth-promoting rhizobacterium paraburkholderia phytofirmans psjn harbors iac gene homologues in its genome, but with a different gene organization and context than those of p. putida 1290. the iac gene functions enable p ... | 2017 | 27795307 |
| host range of the conjugative transfer system of incp-9 naphthalene-catabolic plasmid nah7 and characterization of its orit region and relaxase. | nah7 and pww0 from gammaproteobacterial pseudomonas putida strains are incp-9 conjugative plasmids that carry the genes for degradation of naphthalene and toluene, respectively. although such genes on these plasmids are well-characterized, experimental investigation of their conjugation systems remains at a primitive level. to clarify these conjugation systems, in this study, we investigated the nah7-encoded conjugation system by (i) analyzing the origin of its conjugative transfer (orit)-contai ... | 2017 | 27742684 |
| production of encapsulated creatinase using yeast spores. | yeast spores can be used as a carrier to produce enzyme capsules. in the present study, this technique was applied to a diagnostic enzyme named creatinase. we found that a secretory form of pseudomonas putida creatinase could be entrapped in the spore wall, and such spores were used as creatinase capsules. the activity of the encapsulated creatinase was largely improved by mild spore wall defective mutations, such as dit1 or osw2 deletions. the advantages of this method include the following: en ... | 2016 | 27791465 |
| effect of crc and hfq proteins on the transcription, processing, and stability of the pseudomonas putida crcz srna. | in pseudomonas putida, the hfq and crc proteins regulate the expression of many genes in response to nutritional and environmental cues, by binding to mrnas that bear specific target motifs and inhibiting their translation. the effect of these two proteins is antagonized by the crcz and crcy small rnas (srnas), the levels of which vary greatly according to growth conditions. the crcz and crcy genes are transcribed from promoters pcrcz and pcrcy, respectively, a process that relies on the cbrb tr ... | 2016 | 27777366 |
| characterization of a novel blaimp gene, blaimp-58, using whole genome sequencing in a pseudomonas putida isolate detected in denmark. | a multidrug-resistant strain of pseudomonas putida was isolated from the urine of a 65-year-old women hospitalized for serious clinical conditions. using whole genome sequencing a novel blaimp gene, blaimp-58 was discovered and characterized. | 2017 | 27771209 |
| global transcriptional response of solvent-sensitive and solvent-tolerant pseudomonas putida strains exposed to toluene. | pseudomonas putida strains are generally recognized as solvent tolerant, exhibiting varied sensitivity to organic solvents. pan-genome analysis has revealed that 30% of genes belong to the core-genome of pseudomonas. accessory and unique genes confer high degree of adaptability and capabilities for the degradation and synthesis of a wide range of chemicals. for the use of these microbes in bioremediation and biocatalysis, it is critical to understand the mechanisms underlying these phenotypic di ... | 2017 | 27768818 |
| recombineering and i-scei-mediated pseudomonas putida kt2440 scarless gene deletion. | pseudomonas putida kt2440 is a saprophytic, generally recognized as safe microorganism that plays important roles in the biodegradation and production of value-added chemicals. chromosomal gene deletion of p. putida kt2440 usually involves time-consuming gene coning, conjugal transfer and counterselection. recently, we developed a p. putida kt2440 markerless gene deletion method based on recombineering and cre/loxp site-specific recombination. pcr-based λ red recombineering circumvents the tedio ... | 2016 | 27765807 |
| interactive effects of phosphorus and pseudomonas putida on chickpea (cicer arietinum l.) growth, nutrient uptake, antioxidant enzymes and organic acids exudation. | phosphorus (p) availability in alkaline soils of arid and semi-arid regions is a major constraint for decreased crop productivity. use of plant growth promoting rhizobacteria (pgpr) may enhance plant growth through the increased plant antioxidation activity. additionally, pgpr may increase nutrient uptake by plants as a result of induced root exudation and rhizosphere acidification. the current study was aimed to investigate combined effects of p and pesudomonas putida (pgpr) on chickpea growth ... | 2016 | 27485620 |
| structural basis for the transcriptional repressor nicr2 in nicotine degradation from pseudomonas. | nicotine is an environmental toxicant in tobacco wastes, imposing severe hazards for the health of human and other mammalians. nicr2, a tetr-like repressor from pseudomonas putida s16, plays a critical role in regulating nicotine degradation. here, we determined the crystal structures of nicr2 and its complex with the inducer 6-hydroxy-3-succinoyl-pyridine (hsp). the n-terminal domain of nicr2 contains a conserved helix-turn-helix (hth) dna-binding motif, while the c-terminal domain contains a c ... | 2017 | 27741553 |
| robust at-line quantification of poly(3-hydroxyalkanoate) biosynthesis by flow cytometry using a bodipy 493/503-syto 62 double-staining. | poly(3-hydroxyalkanoates) (phas) are bio-based and biodegradable polyesters which have been considered as a promising alternative to petrol-based plastics. their bacterial production is a dynamic process in which intracellular polymerization and depolymerization are closely linked and depend on the availability of carbon substrates and other nutrients. these dynamics require a fast and quantitative method to determine the optimal harvest-time of pha containing cells or to adjust carbon supply. i ... | 2016 | 27720900 |
| identification and validation of novel small proteins in pseudomonas putida. | small proteins of 50 amino acids or less have been understudied due to difficulties that impede their annotation and detection. in order to obtain information on small open reading frames (sorfs) in pseudomonas putida, bioinformatic and proteomic approaches were used to identify putative sorfs in the well-characterized strain kt2440. a plasmid-based system was established for sorf validation, enabling expression of c-terminal sequential peptide affinity tagged variants and their detection via pr ... | 2016 | 27717237 |
| toxicity evaluation of selected ammonium-based ionic liquid forms with mcpp and dicamba moieties on pseudomonas putida. | combination of the hydrophilic herbicidal anion with hydrophobic, antimicrobial ammonium cation allows to obtain compounds in ionic liquid form with better properties then conventional herbicides. both cation and anion can be modified by selection of herbicide and the length of alkyl chains in cation structure. however the knowledge of their potential toxic effects are still limited. furthermore, the relation between hydrophobicity associated with the length of alkyl chains and toxicity for ioni ... | 2017 | 27716584 |
| stepwise simulation of 3,5-dihydro-5-methylidene-4h-imidazol-4-one (mio) biogenesis in histidine ammonia-lyase. | a 3,5-dihydro-5-methylidene-4h-imidazol-4-one (mio) electrophilic moiety is post-translationally and autocatalytically generated in homotetrameric histidine ammonia-lyase (hal) and other enzymes containing the tripeptide ala-ser-gly in a suitably positioned loop. the backbone cyclization step is identical to that taking place during fluorophore formation in green fluorescent protein from the tripeptide ser-tyr-gly, but dehydration, rather than dehydrogenation by molecular oxygen, is the reaction ... | 2016 | 27682658 |
| expression of the phosphodiesterase bifa facilitating swimming motility is partly controlled by flia in pseudomonas putida kt2440. | flagella-mediated motility is an important capability of many bacteria to survive in nutrient-depleted and harsh environments. decreasing the intracellular cyclic di-gmp (c-di-gmp) level by overexpression of phosphodiesterase bifa promotes flagellar-mediated motility and induces planktonic lifestyle in pseudomonas. the mechanism that regulates expression of bifa gene was poorly studied. here we showed that expression of bifa was partly controlled by flagellar sigma factor flia (σ(28) ) in pseudo ... | 2017 | 27663176 |
| performance of an ultraviolet mutagenetic polyphosphate-accumulating bacterium pz2 and its application for wastewater treatment in a newly designed constructed wetland. | total phosphorus (tp) removal performance and application for wastewater treatment of polyphosphate-accumulating bacteria (pab) in constructed wetlands (cws) were investigated. in this study, a novel isolated ultraviolet (uv) mutant pz2 with phosphate-accumulating ability was screened from domestic wastewater and identified as pseudomonas putida by 16s ribosomal dna (rdna) sequencing analysis. the tp removal performance of pz2 in the synthetic wastewater reached the highest of 93.95 % within 45 ... | 2017 | 27659999 |
| the response of aggregated pseudomonas putida cp1 cells to uv-c and uv-a/b disinfection. | uv radiation is a spread method used worldwide for the disinfection of water. however, much of the research on the disinfection of bacterial cells by uv has focused on planktonic cells. many bacterial cells in nature are present in clumps or aggregates, and these aggregates, which are more resistant to disinfection than their planktonic counterparts, can be problematic in engineered water systems. the current research used pseudomonas putida (p. putida) cp1, an environmental and non-pathogenic m ... | 2016 | 27655528 |
| a pseudo-outbreak of pseudomonas putida and stenotrophomonas maltophilia in a bronchoscopy unit. | endoscopes represent the medical devices most commonly linked to health care-associated outbreaks and pseudo-outbreaks. most of the recent outbreaks and pseudo-outbreaks have resulted from contaminated automated endoscope reprocessors (aer) or the use of damaged or malfunctioning bronchoscopes or contaminated equipment. | 2016 | 27627819 |
| a rapid method to estimate nadh regeneration rates in living cells. | we introduce a rapid whole cell assay for the estimation of nadh regeneration rates based on the fluorescent dye resazurin. a co-feed of formate and glucose, known to increase the intrinsic nadh regeneration rate of p. putida kt2440, was chosen as model system for the validation of this assay. | 2016 | 27592588 |
| structural and kinetic properties of the aldehyde dehydrogenase nahf, a broad substrate specificity enzyme for aldehyde oxidation. | the salicylaldehyde dehydrogenase (nahf) catalyzes the oxidation of salicylaldehyde to salicylate using nad(+) as a cofactor, the last reaction of the upper degradation pathway of naphthalene in pseudomonas putida g7. the naphthalene is an abundant and toxic compound in oil and has been used as a model for bioremediation studies. the steady-state kinetic parameters for oxidation of aliphatic or aromatic aldehydes catalyzed by 6xhis-nahf are presented. the 6xhis-nahf catalyzes the oxidation of ar ... | 2016 | 27580341 |
| competitive adsorption of pb and cd on bacteria-montmorillonite composite. | the characteristics and mechanisms of competitive adsorption of trace metals on bacteria-associated clay mineral composites have never been studied, despite their being among the most common organic-mineral complexes in geological systems. herein, competitive adsorption of pb and cd on pseudomonas putida-montmorillonite composite was investigated through adsorption-desorption experiment, isothermal titration calorimetry (itc), and synchrotron micro x-ray fluorescence (μ-xrf). from the experiment ... | 2016 | 27566847 |
| spatial pattern of copper phosphate precipitation involves in copper accumulation and resistance of unsaturated pseudomonas putida cz1 biofilm. | bacterial biofilms are spatially structured communities that contain bacterial cells with a wide range of physiological states. the spatial distribution and speciation of copper in unsaturated pseudomonas putida cz1 biofilms that accumulated 147.0 mg copper per g dry weight were determined by transmission electron microscopy coupled with energy dispersive x-ray analysis, and micro-x-ray fluorescence microscopy coupled with micro-x-ray absorption near edge structure (micro-xanes) analysis. it was ... | 2016 | 27558435 |
| continuous multistep synthesis of perillic acid from limonene by catalytic biofilms under segmented flow. | the efficiency of biocatalytic reactions involving industrially interesting reactants is often constrained by toxification of the applied biocatalyst. here, we evaluated the combination of biologically and technologically inspired strategies to overcome toxicity-related issues during the multistep oxyfunctionalization of (r)-(+)-limonene to (r)-(+)-perillic acid. pseudomonas putida gs1 catalyzing selective limonene oxidation via the p-cymene degradation pathway and recombinant pseudomonas taiwan ... | 2017 | 27530691 |
| synthesis gas (syngas)-derived medium-chain-length polyhydroxyalkanoate synthesis in engineered rhodospirillum rubrum. | the purple nonsulfur alphaproteobacterium rhodospirillum rubrum s1 was genetically engineered to synthesize a heteropolymer of mainly 3-hydroxydecanoic acid and 3-hydroxyoctanoic acid [p(3hd-co-3ho)] from co- and co2-containing artificial synthesis gas (syngas). for this, genes from pseudomonas putida kt2440 coding for a 3-hydroxyacyl acyl carrier protein (acp) thioesterase (phag), a medium-chain-length (mcl) fatty acid coenzyme a (coa) ligase (pp_0763), and an mcl polyhydroxyalkanoate (pha) syn ... | 2016 | 27520812 |
| cellshape: a user-friendly image analysis tool for quantitative visualization of bacterial cell factories inside. | visualization of the intracellular constituents of individual bacteria while performing as live biocatalysts is in principle doable through more or less sophisticated fluorescence microscopy. unfortunately, rigorous quantitation of the wealth of data embodied in the resulting images requires bioinformatic tools that are not widely extended within the community-let alone that they are often subject to licensing that impedes software reuse. in this context we have developed cellshape, a user-frien ... | 2017 | 27492366 |
| rhizosphere selection of pseudomonas putida kt2440 variants with increased fitness associated to changes in gene expression. | as the interface between plant roots and soil, the rhizosphere is a complex environment where nutrients released by the plant promote microbial growth. increasing evidences indicate that the plant also exerts a selective pressure on microbial populations in the rhizosphere, favouring colonization by certain groups. in this work, we have designed an experimental setup to begin analysing the evolution of a specific bacterial population in the rhizosphere, using pseudomonas putida kt2440 as model o ... | 2016 | 27487165 |
| biosorption of aluminum through the use of non-viable biomass of pseudomonas putida. | living and non-living biomass of pseudomonas putida a (atcc 12633) was used as biosorbent for the removing of al(3+) from aqueous solutions. the process was stable with time, efficient at ph 4.3 and between 15°c and 42°c. two isotherms models were applied to describe the interaction between the biosorbent and al(3+). non-living biomass of p. putida a (atcc 12633) was found to be the most efficient at adsorbing al(3+) with a maximum sorption capacity of 0.55mg al(3+)/gr adsorbent and with 36×10(5 ... | 2016 | 27485814 |
| proteome analysis of pseudomonas putida f1 genes induced in soil environments. | knowledge of the gene expression dynamics of a single soil bacterial strain contributes to the understanding of its behaviour, physiological state and surrounding microenvironment. genes expressed in soil environments rather than in laboratory media are considered to particularly relevant. here, we compared genome-wide gene expression profiles of the bacterium pseudomonas putida f1 inoculated in three different types of nonsterile soils deduced using proteome analysis via sodium dodecyl sulfate- ... | 2016 | 27452675 |
| production of glucaric acid from myo-inositol in engineered pichia pastoris. | a potential myo-inositol oxygenase (ppmiox) was identified as a functional enzyme and a glucaric acid synthetic pathway was firstly constructed in pichia pastoris. coexpression of the native ppmiox and the urinate dehydrogenase (udh) from pseudomonas putida kt2440 led to obvious accumulation of glucaric acid (90.46±0.04mg/l) from myo-inositol whereas no glucaric acid was detected from glucose. in comparison, coexpression of the heterologous mouse miox (mmiox) and udh resulted in higher titers of ... | 2016 | 27444324 |
| content of cardiolipin of the membrane and sensitivity to cationic surfactants in pseudomonas putida. | to establish the role of cardiolipin (cl) of the membrane in response to the presence of tetradecyltrimethylammonium in pseudomonas putida a (atcc 12633). | 2016 | 27442261 |
| self-regulation and interplay of rsm family proteins modulate the lifestyle of pseudomonas putida. | in the plant-beneficial bacterium pseudomonas putida kt2440, three genes have been identified that encode posttranscriptional regulators of the csra/rsma family. their regulatory roles in the motile and sessile lifestyles of p. putida have been investigated by generating single-, double-, and triple-null mutants and by overexpressing each protein (rsma, rsme, and rsmi) in different genetic backgrounds. the rsm triple mutant shows reduced swimming and swarming motilities and increased biofilm for ... | 2016 | 27422830 |
| pseudomonas putida as a platform for the synthesis of aromatic compounds. | aromatic compounds such as l-phenylalanine, 2-phenylethanol and trans-cinnamate are aromatic compounds of industrial interest. current trends support replacement of chemical synthesis of these compounds by 'green' alternatives produced in microbial cell factories. the solvent-tolerant pseudomonas putida dot-t1e strain was genetically modified to produce up to 1 g l-1 of l-phenylalanine. in order to engineer this strain, we carried out the following stepwise process: (1) we selected random mutant ... | 2016 | 27417954 |
| biofilm adaptation to iron availability in the presence of biotite and consequences for chemical weathering. | bacteria in nature often live within biofilms, exopolymeric matrices that provide a favorable environment that can differ markedly from their surroundings. biofilms have been found growing on mineral surfaces and are expected to play a role in weathering those surfaces, but a clear understanding of how environmental factors, such as trace-nutrient limitation, influence this role is lacking. here, we examine biofilm development by pseudomonas putida in media either deficient or sufficient in fe d ... | 2016 | 27384343 |
| role of conserved met112 residue in the catalytic activity and stability of ketosteroid isomerase. | ketosteroid isomerase (3-oxosteroid δ(5)-δ(4)-isomerase, ksi) from pseudomonas putida catalyzes allylic rearrangement of the 5,6-double bond of δ(5)-3-ketosteroid to 4,5-position by stereospecific intramolecular transfer of a proton. the active site of ksi is formed by several hydrophobic residues and three catalytic residues (tyr14, asp38, and asp99). in this study, we investigated the role of a hydrophobic met112 residue near the active site in the catalysis, steroid binding, and stability of ... | 2016 | 27375051 |
| 3-fluorophenmetrazine, a fluorinated analogue of phenmetrazine: studies on in vivo metabolism in rat and human, in vitro metabolism in human cyp isoenzymes and microbial biotransformation in pseudomonas putida and wastewater using gc and lc coupled to (hr)-ms techniques. | wastewater-based epidemiology (wbe) as means to estimate illicit drug and new psychoactive substance (nps) consumption with spatial and temporal resolution is gaining increasing attention. in order to evaluate a given nps using wbe, in vivo metabolism and microbial biotransformation of excretion products and unchanged compounds need evaluation. the aims of this study were to identify in vivo phase i and ii metabolites of the nps 3-fluorophenmetrazine (3-fpm) in human and rat urine and study the ... | 2016 | 27372653 |
| pyridine nucleotide transhydrogenases enable redox balance of pseudomonas putida during biodegradation of aromatic compounds. | the metabolic versatility of the soil bacterium pseudomonas putida is reflected by its ability to execute strong redox reactions (e.g., mono- and di-oxygenations) on aromatic substrates. biodegradation of aromatics occurs via the pathway encoded in the archetypal tol plasmid pww0, yet the effect of running such oxidative route on redox balance against the background metabolism of p. putida remains unexplored. to answer this question, the activity of pyridine nucleotide transhydrogenases (that ca ... | 2016 | 27348295 |
| novel polyhydroxyalkanoate copolymers produced in pseudomonas putida by metagenomic polyhydroxyalkanoate synthases. | bacterially produced biodegradable polyhydroxyalkanoates (phas) with versatile properties can be achieved using different pha synthases (phacs). this work aims to expand the diversity of known phacs via functional metagenomics and demonstrates the use of these novel enzymes in pha production. complementation of a pha synthesis-deficient pseudomonas putida strain with a soil metagenomic cosmid library retrieved 27 clones expressing either class i, class ii, or unclassified pha synthases, and many ... | 2016 | 27333909 |
| in silico analysis for prediction of degradative capacity of pseudomonas putida sf1. | the study employs draft genome sequence data to explore p-nitrophenol (pnp) degradation activity of pseudomonas putida strain sf-1 at a genomic scale. annotation analysis proposes that the strain sf1 not only possesses the gene cluster for pnp utilization but also for the utilization of benzoate, catechol, hydroxybenzoate, protocatechuate, and homogentisate. further, the analysis was carried out to understand more details of pnp 4-monooxygenase and its regulator. a comparative analysis of pnp 4- ... | 2016 | 27317892 |
| signaling states of a short blue-light photoreceptor protein ppsb1-lov revealed from crystal structures and solution nmr spectroscopy. | light-oxygen-voltage (lov) domains represent the photo-responsive domains of various blue-light photoreceptor proteins and are widely distributed in plants, algae, fungi, and bacteria. here, we report the dark-state crystal structure of ppsb1-lov, a slow-reverting short lov protein from pseudomonas putida that is remarkably different from our previously published "fully light-adapted" structure [1]. a direct comparison of the two structures provides insight into the light-activated signaling mec ... | 2016 | 27291287 |
| fusion proteins of an enoate reductase and a baeyer-villiger monooxygenase facilitate the synthesis of chiral lactones. | nature uses the advantages of fusion proteins for multi-step reactions to facilitate the metabolism in cells as the conversion of substrates through intermediates to the final product can take place more rapidly and with less side-product formation. in a similar fashion, also for enzyme cascade reactions, the fusion of biocatalysts involved can be advantageous. in the present study, we investigated fusion of an alcohol dehydrogenase (adh), an enoate reductase (ered) and a baeyer-villiger monooxy ... | 2017 | 27289001 |
| μmore: a microfluidic magnetic oscillation reactor for accelerated parameter optimization in biocatalysis. | enzymatic parameter determination is an essential step in biocatalytic process development. therefore higher throughput in miniaturized devices is urgently needed. an ideal microfluidic device should combine easy immobilization and retention of a minimal amount of biocatalyst with a well-mixed reaction volume. together, all criteria are hardly met by current tools. here we describe a microfluidic reactor (μmore) which employs magnetic particles for both enzyme immobilization and efficient mixing ... | 2016 | 27288595 |
| total growth and root-cluster production by legumes and proteas depends on rhizobacterial strain, host species and nitrogen level. | root clusters are bunches of hairy rootlets produced by >1800 species in nine families. the possible involvement of micro-organisms in root-cluster formation has produced conflicting results over the last 40 years. in addition, any effect of rhizobacteria on overall plant growth of root-cluster-bearing species remains unknown. | 2016 | 27288511 |
| mobilization of pollutant-degrading bacteria by eukaryotic zoospores. | the controlled mobilization of pollutant-degrading bacteria has been identified as a promising strategy for improving bioremediation performance. we tested the hypothesis whether the mobilization of bacterial degraders may be achieved by the action of eukaryotic zoospores. we evaluated zoospores that are produced by the soil oomycete pythium aphanidermatum as a biological vector, and, respectively, the polycyclic aromatic hydrocarbon (pah)-degrading bacteria mycobacterium gilvum vm552 and pseudo ... | 2016 | 27286642 |
| coexistence of two d-lactate-utilizing systems in pseudomonas putida kt2440. | it is advantageous for rhizosphere-dwelling microorganisms to utilize organic acids such as lactate. pseudomonas putida kt2440 is one of the most widely studied rhizosphere-dwelling model organisms. the p. putida kt2440 genome contains an nad-dependent d-lactate dehydrogenase encoding gene, but mutation of this gene does not play a role in d-lactate utilization. instead, it was found that d-lactate utilization in p. putida kt2440 proceeds via a multidomain nad-independent d-lactate dehydrogenase ... | 2016 | 27264531 |
| 3-chloro-1,2-propanediol biodegradation by ca-alginate immobilized pseudomonas putida dsm 437 cells applying different processes: mass transfer effects. | 3-chloro-1,2-propanediol (3-cpd) biodegradation by ca-alginate immobilized pseudomonas putida cells was performed in batch system, continuous stirred tank reactor (cstr), and packed-bed reactor (pbr). batch system exhibited higher biodegradation rates and 3-cpd uptakes compared to cstr and pbr. the two continuous systems (cstr and pbr) when compared at 200 mg/l 3-cpd in the inlet exhibited the same removal of 3-cpd at steady state. external mass-transfer limitations are found negligible at all s ... | 2016 | 27262716 |
| nitrile-synthesizing enzyme: screening, purification and characterization. | cyanide is known as a toxic compound for almost all living organisms. we have searched for cyanide-resistant bacteria from the soil and stock culture collection of our laboratory, and have found the existence of a lot of microorganisms grown on culture media containing 10 mm potassium cyanide. almost all of these cyanide-resistant bacteria were found to show β-cyano-l-alanine (β-cnala) synthetic activity. β-cnala synthase is known to catalyze nitrile synthesis: the formation of β-cnala from pota ... | 2016 | 27250663 |
| from dirt to industrial applications: pseudomonas putida as a synthetic biology chassis for hosting harsh biochemical reactions. | the soil bacterium pseudomonas putida is endowed with a central carbon metabolic network capable of fulfilling high demands of reducing power. this situation arises from a unique metabolic architecture that encompasses the partial recycling of triose phosphates to hexose phosphates-the so-called edemp cycle. in this article, the value of p. putida as a bacterial chassis of choice for contemporary, industrially-oriented metabolic engineering is addressed. the biochemical properties that make this ... | 2016 | 27239751 |
| complete biodegradation of chlorpyrifos by engineered pseudomonas putida cells expressing surface-immobilized laccases. | the long-term abuse use of chlorpyrifos-like pesticides in agriculture and horticulture has resulted in significant soil or water contamination and a worldwide ecosystem threat. in this study, the ability of a solvent-tolerant bacterium, pseudomonas putida mb285, with surface-displayed bacterial laccase, to biodegrade chlorpyrifos was investigated. the results of compositional analyses of the degraded products demonstrate that the engineered mb285 was capable of completely eliminating chlorpyrif ... | 2016 | 27231878 |
| impact of pnpr, a lysr-type regulator-encoding gene, on the cellular processes of pseudomonas putida dll-e4. | lysr-type transcriptional regulators (lttrs) regulate various cellular processes in bacteria. pnpr is an lttr-encoding gene involved in the regulation of hydroquinone (hq) degradation, and its effects on the cellular processes of pseudomonas putida dll-e4 were investigated at the physiological, biochemical and molecular levels. reverse transcription polymerase chain reaction revealed that pnpr positively regulated its own expression and that of the pnpc1c2decx1x2 operon; additionally, pnpr parti ... | 2016 | 27190157 |
| biofilm formation-defective mutants in pseudomonas putida. | out of 8000 candidates from a genetic screening for pseudomonas putida kt2442 mutants showing defects in biofilm formation, 40 independent mutants with diminished levels of biofilm were analyzed. most of these mutants carried insertions in genes of the lap cluster, whose products are responsible for synthesis, export and degradation of the adhesin lapa. all mutants in this class were strongly defective in biofilm formation. mutants in the flagellar regulatory genes fleq and flhf showed similar d ... | 2016 | 27190143 |
| u(vi) biosorption by bi-functionalized pseudomonas putida @ chitosan bead: modeling and optimization using rsm. | in this work, pseudomonas putida cells immobilized into chitosan beads (picb) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for u(vi) biosorption from aqueous solutions. response surface methodology (rsm) based on central composite design (ccd) was utilized to evaluate the performance of the picb in comparison with chitosan beads (cb) under batch mode. performing experiments under optimal condition sets viz. ph 5, initial u( ... | 2016 | 27180295 |
| sulfhydryl binding sites within bacterial extracellular polymeric substances. | in this study, the concentration of sulfhydryl sites on bacterial biomass samples with and without extracellular polymeric substances (eps) was measured in order to determine the distribution of sulfhydryl sites on bacteria. three different approaches were employed for eps removal from pseudomonas putida, and the measured sulfhydryl concentrations on bacterial eps molecules are independent of the eps removal protocols used. prior to eps removal, the measured sulfhydryl sites within p. putida sam ... | 2016 | 27177017 |
| enhancing indigo production by over-expression of the styrene monooxygenase in pseudomonas putida. | as an important traditional blue dye, indigo has been used in food and textile industry for centuries, which can be produced via the styrene oxygenation pathway in pseudomonas putida. hence, the styrene monooxygenase gene styab and oxide isomerase gene styc are over-expressed in p. putida to investigate their roles in indigo biosynthesis. rt-qpcr analysis indicated that transcriptions of stya and styb were increased by 2500- and 750-folds in the styab over-expressed strain b4-01, compared with t ... | 2016 | 27154464 |
| c-di-gmp regulates the expression of lapa and bcs operons via fleq in pseudomonas putida kt2440. | cyclic diguanylate (c-di-gmp) positively modulates the production of biofilm matrix components from the transcriptional to the post-translational level in a variety of bacterial species. however, mechanisms by which it regulates these opponents in pseudomonas putida kt2440 remain unclear. here we show that c-di-gmp regulates the adhesin lapa, lapf and exopolysaccharides bcs, pea at transcriptional level. transcriptional regulator fleq is required for the modulation of lapa and bcs expression by ... | 2016 | 27120564 |
| bio-statistical enhancement of acyl transfer activity of amidase for biotransformation of n-substituted aromatic amides. | acyl transfer activity (ata) of amidase transfers an acyl group of different amides to hydroxylamine to form the corresponding hydroxamic acid. bacterial isolate br-1 was isolated from cyanogenic plant cirsium vulgare rhizosphere and identified as pseudomonas putida br-1 by 16s rdna sequencing. this organism exhibited high ata for the biotransformation of n-substituted aromatic amide to the corresponding hydroxamic acid. optimization of media, tryptone (0.6%), inducer, ph 8.5, and a growth tempe ... | 2016 | 27118077 |
| genome-wide mapping of transcription start sites yields novel insights into the primary transcriptome of pseudomonas putida. | the environmental bacterium pseudomonas putida is an organism endowed with a versatile metabolism and stress tolerance traits that are desirable in an efficient production organism. in this work, differential rna sequencing was used to investigate the primary transcriptome and rna regulatory elements of p. putida strain kt2440. a total of 7937 putative transcription start sites (tsss) were identified, where over two-thirds were located either on the opposite strand or internal to annotated genes ... | 2016 | 27111755 |
| broad-host-range prouser vectors enable fast characterization of inducible promoters and optimization of p-coumaric acid production in pseudomonas putida kt2440. | pseudomonas putida kt2440 has gained increasing interest as a host for the production of biochemicals. because of the lack of a systematic characterization of inducible promoters in this strain, we generated prouser broad-host-expression plasmids that facilitate fast uracil-based cloning. a set of prouser-reporter vectors was further created to characterize different inducible promoters. the prhab and pm promoters were orthogonal and showed titratable, high, and homogeneous expression. to optimi ... | 2016 | 27092814 |
| a pseudomonas putida cbrb transposon insertion mutant displays a biofilm hyperproducing phenotype that is resistant to dispersal. | the cbrab two-component system in the pseudomonads controls a variety of metabolic and behavioural traits required for its adaptation to changing environmental conditions, including the uptake or assimilation of certain carbon sources, and processes such as chemotaxis or stress tolerance. in this work we characterise a minitn5-luxab-km transposon insertion mutant in cbrb (mpo406) in pseudomonas putida leading to a biofilm overproducing phenotype that is not dispersed when nutrients are depleted. ... | 2016 | 27085034 |
| methylmercury degradation by pseudomonas putida v1. | environmental contamination of mercury (hg) has caused public health concerns with focuses on the neurotoxic substance methylmercury, due to its bioaccumulation and biomagnification in food chains. the goals of the present study were to examine: (i) the transformation of methylmercury, thimerosal, phenylmercuric acetate and mercuric chloride by cultures of pseudomonas putida v1, (ii) the presence of the genes mera and merb in p. putida v1, and (iii) the degradation pathways of methylmercury by p ... | 2016 | 27062344 |
| ventosimonas gracilis gen. nov., sp. nov., a member of the gammaproteobacteria isolated from cephalotes varians ant guts representing a new family, ventosimonadaceae fam. nov., within the order 'pseudomonadales'. | cephalotes 'turtle' ants are known to harbor a core group of gut symbionts, including members belonging to the gammaproteobacteria. here, we describe the cultivation and characterization of strain cv58t, a novel member of the gammaproteobacteria order pseudomonadales isolated from the guts of the ant cephalotes varians. strain cv58t was rod-shaped, gram-stain-negative, non-motile and formed pale-yellow colonies on trypticase soy agar. optimum growth occurred under an atmosphere of 4-20 % (v/v) o ... | 2016 | 27054961 |
| transcriptional kinetics of the cross-talk between the ortho-cleavage and tol pathways of toluene biodegradation in pseudomonas putida mt-2. | the tol plasmid promoters are activated by toluene leading to gene expression responsible for the degradation of the environmental signal. benzoate is formed as an intermediate, activating the benr protein of the chromosomal ortho-cleavage pathway that up-regulates the chromosomal pbena promoter and the tol pm promoter resulting in cross-talk between the two networks. herein, the transcriptional kinetics of the pbenr and pbena promoters in conjunction with tol promoters was monitored by real-tim ... | 2016 | 27046069 |
| loop-grafted old yellow enzymes in the bienzymatic cascade reduction of allylic alcohols. | the enzymatic reduction of c=c bonds in allylic alcohols with old yellow enzymes represents a challenging task, due to insufficient activation through the hydroxy group. in our work, we coupled an alcohol dehydrogenase with three wild-type ene reductases-namely nicotinamide-dependent cyclohex-2-en-1-one reductase (ncr) from zymomonas mobilis, oye1 from saccharomyces pastorianus and morphinone reductase (mr) from pseudomonas putida m10-and four rationally designed β/α loop variants of ncr in the ... | 2016 | 27037735 |
| pseudomonas putida biofilm dynamics following a single pulse of silver nanoparticles. | pseudomonas putida mono-species biofilms were exposed to silver nanoparticles (ag nps) in artificial wastewater (aw) under hydrodynamic conditions. specifically, 48 h old biofilms received a single pulse of ag nps at 0, 0.01, 0.1, 1, 10 and 100 mg l(-1) for 24 h in confocal laser scanning microscopy (clsm) compatible flow-cells. the biofilm dynamics (in terms of morphology, viability and activity) were characterised at 48, 72 and 96 h. consistent patterns were found across flow-cells and experim ... | 2016 | 27031799 |
| numerical modelling of biophysicochemical effects on multispecies reactive transport in porous media involving pseudomonas putida for potential microbial enhanced oil recovery application. | ph and resident time of injected slug plays a critical role in characterizing the reservoir for potential microbial enhanced oil recovery (meor) application. to investigate meor processes, a multispecies (microbes-nutrients) reactive transport model in porous media was developed by coupling kinetic and transport model. the present work differs from earlier works by explicitly determining parametric values required for kinetic model by experimental investigations using pseudomonas putida at diffe ... | 2016 | 27030954 |
| comparative study of wastewater treatment and nutrient recycle via activated sludge, microalgae and combination systems. | algal-bacterial synergistic cultivation could be an optional wastewater treatment technology in temperate areas. in this study, a locally screened vigorous chlorella strain was characterized and then it was used in a comparative study of wastewater treatment and nutrient recycle assessment via activated sludge (as), microalgae and their combination systems. chlorella sp. cultured with as in light showed the best performance, in which case the removal efficiencies of cod, nh3-n and tp were 87.3%, ... | 2016 | 26995615 |
| tactic response of bacteria to zero-valent iron nanoparticles. | the microbial assessment of pollutant toxicity rarely includes behavioral responses. in this study, we investigated the tactic response of pseudomonas putida g7, a representative of soil bacterium, towards engineered zero-valent iron nanoparticles (nzvis), as a new end-point assessment of toxicity. the study integrated the characterization of size distribution and charge of nzvis and tactic reaction response by means of inverted capillary assay and computer-assisted motion analysis of motility b ... | 2016 | 26967351 |
| rationally rewiring the connectivity of the xylr/pu regulatory node of the m-xylene degradation pathway in pseudomonas putida. | the xylr/pu regulatory node of the m-xylene biodegradation pathway of pseudomonas putida mt-2 is one of the most intricate cases of processing internal and external cues into a single controlling element. despite this complexity, the performance of the regulatory system is determined in vivo only by the occupation of pu by m-xylene-activated xylr and σ(54)-rnap. the stoichiometry between these three elements defines natural system boundaries that outline a specific functional space. this space c ... | 2016 | 26961967 |
| photoactivation reduces side-chain dynamics of a lov photoreceptor. | we used neutron-scattering experiments to probe the conformational dynamics of the light, oxygen, voltage (lov) photoreceptor ppsb1-lov from pseudomonas putida in both the dark and light states. global protein diffusion and internal macromolecular dynamics were measured using incoherent neutron time-of-flight and backscattering spectroscopy on the picosecond to nanosecond timescales. global protein diffusion of ppsb1-lov is not influenced by photoactivation. observation-time-dependent global dif ... | 2016 | 26958884 |
| airborne toluene removal for minimizing occupational health exposure by means of a trickle-bed biofilter. | the paper presents the experimental results on a biotrickling pilot plant, with a water scrubber as pre-treatment, finalized to the treatment of an airborne toluene stream in a working place. the air stream was characterized by a very high variability of the inlet concentrations of toluene (range: 4.35-68.20 mg nm(-3)) with an average concentrations of 16.41 mg nm(-3). the pilot plant has proved its effectiveness in toluene removal, along a 90-day experimentation period, in steady-state conditio ... | 2016 | 26946504 |
| biological manganese oxidation by pseudomonas putida in trickling filters. | biological oxidation has been researched as a viable alternative for treating waters with high manganese (mn) concentrations, typically found in mine drainage or in some geological formations. in this study, laboratory-scale trickling filters were constructed to compare the mn removal efficiency between filters inoculated with the mn oxidizing bacteria, pseudomonas putida, and filters without inoculation. manganese oxidation and removal was found to be significantly greater in trickling filters ... | 2016 | 26943637 |