| draft genome sequences of yersinia frederiksenii, yersinia intermedia, and yersinia kristensenii strains from brazil. | yersinia enterocolitica-like strains are usually understudied. in this work, we reported the draft genome sequences of two yersinia frederiksenii, two yersinia intermedia, and two yersinia kristensenii strains isolated from humans, animals, food, and the environment in brazil. these draft genomes will provide better molecular characterizations of these species. | 2017 | 28798182 |
| engineering of yersinia phytases to improve pepsin and trypsin resistance and thermostability and application potential in the food and feed industry. | susceptibility to proteases usually limits the application of phytase. we sought to improve the pepsin and trypsin resistance of yeappa from yersinia enterocolitica and ykappa from y. kristensenii by optimizing amino acid polarity and charge. the predicted pepsin/trypsin cleavage sites f89/k226 in pepsin/trypsin-sensitive yeappa and the corresponding sites (f89/e226) in pepsin-sensitive but trypsin-resistant ykappa were substituted with s and h, respectively. six variants were produced in pichia ... | 2017 | 28752758 |
| galectin-1-driven tolerogenic programs aggravate yersinia enterocolitica infection by repressing antibacterial immunity. | yersinia enterocolitica is an enteropathogenic bacterium that causes gastrointestinal disorders, as well as extraintestinal manifestations. to subvert the host's immune response, y. enterocolitica uses a type iii secretion system consisting of an injectisome and effector proteins, called yersinia outer proteins (yops), that modulate activation, signaling, and survival of immune cells. in this article, we show that galectin-1 (gal-1), an immunoregulatory lectin widely expressed in mucosal tissues ... | 2017 | 28716827 |
| secondary focal form of yersinia enterocolitica infection with prolonged polyarthritis in young caucasian male: a case report. | current issue deals with an interesting clinical case of a rare infectious disease in a caucasian young male patient, caused by yersinia enterocоlitica. infection proceeded in the development of secondary focal form, which was accompanied by prolonged polyarthritis. we described a clinical case of secondary focal form with prolonged polyarthritis caused by y. enterocolitica o:3 serogroup in young patient with the purpose of focusing on the early clinical and laboratory diagnosistics of yersinios ... | 2017 | 28711901 |
| development of a novel multiplexed qpcr and pyrosequencing method for the detection of human pathogenic yersiniae. | the purpose of this study was to develop a novel and robust molecular assay for the detection of human pathogenic yersiniae (i.e. yersinia enterocolitica, y. pseudotuberculosis and y. pestis) in complex food samples. the assay combines multiplexed real-time pcr (qpcr) and pyrosequencing for detecting and differentiating human pathogenic yersiniae with high confidence through sequence based confirmation. the assay demonstrated 100% specificity and inclusivity when tested against a panel of 14 y. ... | 2017 | 28704728 |
| a dynamic and adaptive network of cytosolic interactions governs protein export by the t3ss injectisome. | many bacteria use a type iii secretion system (t3ss) to inject effector proteins into host cells. selection and export of the effectors is controlled by a set of soluble proteins at the cytosolic interface of the membrane spanning type iii secretion 'injectisome'. combining fluorescence microscopy, biochemical interaction studies and fluorescence correlation spectroscopy, we show that in live yersinia enterocolitica bacteria these soluble proteins form complexes both at the injectisome and in th ... | 2017 | 28653671 |
| lactoferrin protects against intestinal inflammation and bacteria-induced barrier dysfunction in vitro. | the iron-binding glycoprotein lactoferrin (lf) is naturally present in human breast milk. several studies suggest that lf contributes to infant health and development owing to a variety of protective effects, including antimicrobial and anti-inflammatory features. therefore, we aimed to elucidate its protective properties on intestinal epithelial barrier dysfunction induced by infection or inflammation using the human epithelial cell culture models ht-29/b6 and t84. during barrier perturbation i ... | 2017 | 28614589 |
| thirty years of human infections caused by yersinia enterocolitica in northern spain: 1985-2014. | yersinia enterocolitica infection is a zoonosis with worldwide distribution, gastroenteritis being by far the most common clinical manifestation of human infection. in gipuzkoa, northern spain, human y. enterocolitica infections increased from the mid-1980s to the beginning of the 21st century (from 7·9 to 23·2 annual episodes per 100 000 population) to decrease to 7·2 annual episodes per 100 000 population in the last years of the study. the hospital admission rate due to yersiniosis during the ... | 2017 | 28578732 |
| next-generation sequencing virulome analysis of a yersinia enterocolitica subsp. palearctica bioserotype 4/o:3 st18 isolated from human blood in brazil. | yersinia enterocolitica is a widespread gram-negative bacterium that causes gastrointestinal disease and other clinical manifestations in humans. potentially pathogenic y. enterocolitica has been isolated in brazil, from human, environmental, food, and animal sources. herein we report a genome sequence of y. enterocolitica subsp. palearctica strain ye 19, serotype o:3, biotype 4, sequence type 18, with virulence determinants isolated from human blood in rio de janeiro in 2005. the results corrob ... | 2017 | 28571687 |
| antimicrobial resistance and plasmid replicons in yersinia enterocolitica strains isolated in brazil in 30 years. | some studies evaluated the resistance profile of the y. enterocolitica strains isolated in diverse countries. however, in brazil the isolation and the study of y. enterocolitica are not common and therefore information about the antimicrobial resistance profile of this species in this country is scarce. therefore, the aim of this study was to evaluate the antimicrobial resistance of y. enterocolitica of biotypes 1a, 2 and 4 isolated from clinical and non-clinical sources between 1979 and 2012, i ... | 2017 | 28558260 |
| development of an lps-based elisa for diagnosis of yersinia enterocolitica o:3 infections in danish patients: a follow-up study. | the bacterium yersinia enterocolitica causes gastroenteritis in humans. the study aimed to develop a diagnostic enzyme-linked immunosorbent assay (elisa) for detection of yersinia enterocolitica o:3 lps antibodies in sera from danish patients with suspected yersinia enterocolitica o:3 gastrointestinal infection. as a part of this, antibody decay profiles after culture confirmed yersinia enteritis were studied. | 2017 | 28545413 |
| genetic diversity, virulotyping and antimicrobial resistance susceptibility of yersinia enterocolitica isolated from pigs and porcine products in malaysia. | the objectives of the present study were to determine the antimicrobial resistance, virulotypes and genetic diversity of yersinia enterocolitica isolated from uncooked porcine food and live pigs in malaysia. | 2017 | 28542807 |
| comparison of growth and the cytokines induced by pathogenic yersinia enterocolitica bio-serotypes 3/o: 3 and 2/o: 9. | pathogenic yersinia enterocolitica is widely distributed in china where the primary bio-serotypes are 3/o: 3 and 2/o: 9. recently, the distribution of 2/o: 9 strains are being gradually replaced by 3/o: 3 strains where presently 3/o: 3 strains are the major pathogenic y. enterocolitica in china. to identify the growth conditions and cytokines induced by y. enterocolitica and providing some clues for this shift, we performed competitive growth in vitro and in vivo for these two bio-serotype strai ... | 2017 | 28507952 |
| reactive arthritis caused by yersinia enterocolitica enteritis. | we report a case of reactive arthritis (rea) triggered by yersinia enterocolitica enteritis. a 24-year-old japanese man developed polyarthritis in the lower limbs. two weeks prior to these symptoms, he noted diarrhea, right lower abdominal pain and a fever. y. enterocolitica was not isolated from a stool culture; however, he was diagnosed with rea based on the colonoscopic findings of a high anti-y. enterocolitica antibody titer and hla-b27 antigen positivity. following treatment with methotrexa ... | 2017 | 28502944 |
| an improved kinetic assay for the characterization of metal-dependent pectate lyases. | pectate lyases are a subset of polysaccharide lyases (pls) that specifically utilize a metal dependent β-elimination mechanism to cleave glyosidic bonds in homogalacturonan (hg; α-d-1,4-galacturonic acid). most commonly, pls harness calcium for catalysis; however, some pl families (e.g., pl2 and pl22) display preferences for transitional metals. deploying alternative metals during β-elimination is correlated with signature coordination pocket chemistry, and is reflective of the evolution, functi ... | 2017 | 28417359 |
| draft genome sequences of four yersinia enterocolitica strains, isolated from wild ungulate carcasses. | this study describes the draft genome sequences of four yersinia enterocolitica strains, originally isolated from ungulate carcasses. these isolates were typed biochemically and two were determined to be highly virulent (biotype 1b). the draft genome sequences had a mean size of 4.77 mb and a mean g+c content of 47.1%. | 2017 | 28408689 |
| low prevalence of human enteropathogenic yersinia spp. in brown rats (rattus norvegicus) in flanders. | brown rats (rattus norvegicus) have been identified as potential carriers of yersinia enterocolitica and y. pseudotuberculosis, the etiological agents of yersiniosis, the third most reported bacterial zoonosis in europe. enteropathogenic yersinia spp. are most often isolated from rats during yersiniosis cases in animals and humans, and from rats inhabiting farms and slaughterhouses. information is however lacking regarding the extent to which rats act as carriers of these yersinia spp.. in 2013, ... | 2017 | 28403184 |
| evaluation of virulence genes in yersinia enterocolitica strains using sybr green real-time pcr. | yersinia enterocolitica comprises six biotypes 1a, 1b, 2, 3, 4, and 5. the virulence of the strains belonging to biotypes 1b and 2-5 depends on the presence of both chromosomal and plasmid-borne genes. strains belonging to biotype 1a do not carry the virulence plasmid pyv. however, they carry other virulence genes, such as ystb and hrep. the aim of this study was to investigate the distribution of yada, virf, inv, ysta, ystb, myfa, hrep and ymoa virulence genes in y. enterocolitica strains in or ... | 2017 | 28400007 |
| role of the transcriptional activator, virf, and temperature in the expression of the pyv plasmid genes of yersinia enterocolitica. | upon incubation at 37°c in the absence of ca(2+) ions, pathogenic yersiniae release high amounts of pyv plasmid-encoded proteins called yops, involved in pathogenesis. yersinia enterocolitica also express two outer membrane proteins, an adhesin called yada and a lipoprotein called yipa. the production of the yops is co-ordinately regulated by a 20kb region of the plasmid referred to as the 'ca(2+) dependence region' and containing at least four loci called vira, virb, virc, and virf. the 8.5kb v ... | 1992 | 28776801 |