| effect of multispecies probiotics on irritable bowel syndrome: a randomized, double-blind, placebo-controlled trial. | the efficacy of treatment with multispecies probiotics on irritable bowel syndrome (ibs) symptoms and the alterations of gut microbiota in patients who have taken probiotics were investigated. | 2014 | 23829297 |
| exposure of bifidobacterium longum subsp. infantis to milk oligosaccharides increases adhesion to epithelial cells and induces a substantial transcriptional response. | in this study, we tested the hypothesis that milk oligosaccharides may contribute not only to selective growth of bifidobacteria, but also to their specific adhesive ability. human milk oligosaccharides (3'sialyllactose and 6'sialyllactose) and a commercial prebiotic (beneo orafti p95; oligofructose) were assayed for their ability to promote adhesion of bifidobacterium longum subsp. infantis atcc 15697 to ht-29 and caco-2 human intestinal cells. treatment with the commercial prebiotic or 3'sialy ... | 2013 | 23805302 |
| strain-dependent augmentation of tight-junction barrier function in human primary epidermal keratinocytes by lactobacillus and bifidobacterium lysates. | in this study, we investigated whether probiotic lysates can modify the tight-junction function of human primary keratinocytes. the keratinocytes were grown on cell culture inserts and treated with lysates from bifidobacterium longum, lactobacillus plantarum, lactobacillus reuteri, lactobacillus fermentum, or lactobacillus rhamnosus gg. with the exception of l. fermentum (which decreased cell viability), all strains markedly enhanced tight-junction barrier function within 24 h, as assessed by me ... | 2013 | 23770906 |
| [distribution of genes of toxin-antitoxin systems of mazef and relbe families in bifidobacteria from human intestinal microbiota]. | the in silico analysis of 36 sequenced genomes of bacteria of the bifidobacterium genus determined the presence of 19 genes of toxin-antitoxin (ta) systems that belong to the mazef and relbe families, including five mazf and two rele genes that encode toxins and 12 relb genes that encode antitoxins. a high level ofgene (at the level of nucleotide changes) and genomic (presence or absence of genes in distinct genomes) polymorphism in the investigated genes was revealed. the highest level of polym ... | 2013 | 23755531 |
| perinatal pet exposure, faecal microbiota, and wheezy bronchitis: is there a connection? | background. the hygiene hypothesis suggests that high hygiene standards have led to an immune dysfunction and an increase in allergic diseases. farming-related exposures are associated with a decreased risk of asthma. since the gut microbiota may be a pivotal component in the hygiene hypothesis, we studied whether perinatal exposure to pets, doctor's diagnosed wheezy bronchitis (wb), and compositional changes in the gut microbiota are interrelated among urban infants. methods. data were collecte ... | 2013 | 23724248 |
| the applications of polysaccharides from various mushroom wastes as prebiotics in different systems. | the bases or stipes of mushrooms are normally discarded as low-economic value animal feed and compost. there are no known reports on deriving polysaccharides from these mushroom wastes for use as prebiotics. this study showed that the relatively low concentration (0.1% to 0.5%) of polysaccharides from lentinula edodes stipe, pleurotus eryngii base, and flammulina velutipes base can enhance the survival rate of lactobacillus acidophilus, lactobacillus casei, and bifidobacterium longum subsp. long ... | 2013 | 23701736 |
| draft genome sequences of two pairs of human intestinal bifidobacterium longum subsp. longum strains, 44b and 1-6b and 35b and 2-2b, consecutively isolated from two children after a 5-year time period. | we report the genome sequences of four isolates of a human gut symbiont, bifidobacterium longum. strains 44b and 35b were isolated from two 1-year-old infants, while 1-6b and 2-2b were isolated from the same children 5 years later. the sequences permit investigations of factors enabling long-term colonization of bifidobacteria. | 2013 | 23682142 |
| bifidobacterium longum ncc3001 inhibits ah neuron excitability. | bifidobacterium longum (b. longum) ncc3001 can affect behavior and brain biochemistry, but identification of the cellular targets needs further investigation. our hypothesis was that the communication with the brain might start with action on enteric sensory neurons. | 2013 | 23663494 |
| oral administration of bifidobacterium longum cect 7347 ameliorates gliadin-induced alterations in liver iron mobilisation. | coeliac disease is an autoimmune disorder triggered by gluten intake, causing intestinal inflammation and mucosal damage commonly associated with the malabsorption of nutrients and ferropenic anaemia. the present study evaluates the effects of the oral administration of bifidobacterium longum cect 7347 on gliadin-mediated alterations in hepatic fe deposition and hb concentration, liver transferrin receptor (tfr)-2, il-6, tnf-α and hepcidin (hamp) expression (mrna), and active hepcidin peptide pr ... | 2013 | 23657133 |
| [the role of small bowel microflora in the development of secondary lactase deficiency and the possibilities of its treatment with probiotics]. | to estimate the incidence of secondary lactase deficiency (sld) in patients with postinfectious irritable bowel syndrome (pibs) and the value of the small bowel microflora in its development and to elaborate treatment options for sld. | 2013 | 23653934 |
| faecalibacterium prausnitzii upregulates regulatory t cells and anti-inflammatory cytokines in treating tnbs-induced colitis. | faecalibacterium prausnitzii (f. prausnitzii) is a common anaerobic bacteria colonized in the human gut and inflammatory bowel disease (ibd) patients are usually lack of f. prausnitzii. the aims of this study were to evaluate the anti-inflammatory and immunomodulatory capacity of f. prausnitzii by comparing it with bifidobacterium longum (b. longum) in both cellular and animal experiments. | 2013 | 23643066 |
| preparation of n,o-carboxymethyl chitosan coated alginate microcapsules and their application to bifidobacterium longum bioma 5920. | in order to greatly improve vitality of probiotic bacteria within the application, a novel biocompatible vehicle, n,o-carboxymethyl chitosan (nocs) with appropriate degrees of substitution coat alginate (alg) microparticles, was prepared by electrostatic droplet generation. the amount of chitosan (cs) and n,o-carboxymethyl chitosan (nocs) coated on the alg microparticles was determined by differential scanning calorimetry. the surface morphology of alg microparticles, cs coated alg microparticle ... | 2013 | 23623131 |
| modulatory effects of bifidobacterium longum bb536 on defecation in elderly patients receiving enteral feeding. | to investigate the effects of the probiotic bifidobacterium longum bb536 on the health management of elderly patients receiving enteral feeding. | 2013 | 23599642 |
| probiotic administration in congenital heart disease: a pilot study. | to investigate the impact of probiotic bifidobacterium longum ssp. infantis on the fecal microbiota and plasma cytokines in neonates with congenital heart disease. | 2013 | 23599119 |
| tolerance of bifidobacterium human isolates to bile, acid and oxygen. | bifidobacteria are part of the human gastrointestinal microbiota and are used as probiotics in functional food products because of their health promoting properties. however, only few data are available on the phenotypic characteristics displayed by human bifidobacteria strain populations. in this study we compared the in vitro tolerance to acid, bile and oxygen of the largest number of independent human intestinal strains. bile and acid tolerance varied among species and independent strains wit ... | 2013 | 23598280 |
| comparative proteome analysis of bifidobacterium longum subsp. infantis grown on β-glucans from different sources and a model for their utilization. | recent studies have demonstrated that β-glucans from different sources, which are considered as potential prebiotics, could enhance growth of bifidobacteria. to elucidate the metabolic pathway of β-glucans in the widely used probiotic b. longum subsp. infantis, a comparative proteomic analysis was carried out along with two-dimensional difference gel electrophoresis (2d-dige), real-time rt-pcr, and enzyme activity assay on samples obtained from cultures grown on β-glucans derived from barley, se ... | 2013 | 23577653 |
| probiotic administration reduces mortality and improves intestinal epithelial homeostasis in experimental sepsis. | recent clinical trials indicate that probiotic administration in critical illness has potential to reduce nosocomial infections and improve clinical outcome. however, the mechanism(s) of probiotic-mediated protection against infection and sepsis remain elusive. the authors evaluated the effects of lactobacillus rhamnosus gg (lgg) and bifidobacterium longum (bl) on mortality, bacterial translocation, intestinal epithelial homeostasis, and inflammatory response in experimental model of septic peri ... | 2013 | 23571641 |
| structures of cell-wall phosphate-containing glycopolymers of bifidobacterium longum bim b-476-d. | glycopolymers with oligosaccharyl phosphate repeats of two types and ribitol and glycerol teichoic acids were isolated from cell wall of bifidobacterium longum bim b-476-d by stepwise extraction with 10% ccl3co2h. the following structures of the glycopolymers were established by sugar analysis, selective cleavage with aq 2% hoac, dephosphorylation with 48% hf, 2d nmr spectroscopy, and high-resolution esi ms: [structure: see text]. the ribitol teichoic acid also contains minor d-alanine, whose po ... | 2013 | 23571164 |
| development of an ion-exchange chromatography method for monitoring the degradation of prebiotic arabinoxylan-oligosaccharides in a complex fermentation medium. | arabinoxylan-oligosaccharides (axos) are a new class of prebiotics with promising health-promoting characteristics. however, the mechanism by which bacteria break down these compounds in the colon is still uncharacterized, due to their structural complexity. a new analytical method that offers structural information was developed to characterize axos degradation during fermentation. the method was based on the simultaneous determination of arabinose, xylose, xylo-oligosaccharides (xos), and axos ... | 2013 | 23541153 |
| crystal structure of a type ii dehydroquinate dehydratase-like protein from bifidobacterium longum. | dehydroquinate dehydratase (dhqd) catalyzes the third step in the biosynthetic shikimate pathway. here we identify a bifidobacterium longum protein with high sequence homology to type ii dhqds but no detectable dhqd activity under standard assay conditions. a crystal structure reveals that the b. longum protein adopts a dhqd-like tertiary structure but a distinct quaternary state. apparently forming a dimer, the b. longum protein lacks the active site aspartic acid contributed from a neighboring ... | 2013 | 23539270 |
| faecal levels of bifidobacterium and clostridium coccoides but not plasma lipopolysaccharide are inversely related to insulin and homa index in women. | the abundance of specific microbes might be associated with plasma lipopolysaccharide and insulin levels. the aims were to quantify the abundance of specific microbes and plasma lps in females and assess their association with anthropometric, body composition and biochemical measurements. | 2013 | 23538004 |
| physicochemical properties of scamorza ewe milk cheese manufactured with different probiotic cultures. | the present study was undertaken to produce functional scamorza cheese from gentile di puglia ewe milk by incorporating probiotic strains into the cheese matrix and to evaluate the physicochemical characteristics of scamorza ewe milk cheese. gentile di puglia ewe bulk milk was used for scamorza cheese production. cheeses were denoted s-co for control scamorza cheese, s-bb for scamorza cheese made using a mix of bifidobacterium longum and bifidobacterium lactis, and s-la for scamorza cheese made ... | 2013 | 23522678 |
| composition and sensory profiling of probiotic scamorza ewe milk cheese. | the present study aimed to assess the effect of the addition of different usually recognized as probiotic bacterial strains on chemical composition and sensory properties of scamorza cheese manufactured from ewe milk. to define the sensory profile of scamorza cheese, a qualitative and quantitative reference frame specific for a pasta filata cheese was constructed. according to the presence of probiotic bacteria, cheeses were denoted s-bb for scamorza cheese made using a mix of bifidobacterium lo ... | 2013 | 23522674 |
| application of protective cultures against listeria monocytogenes and campylobacter jejuni in chicken products packaged under modified atmosphere. | to control the growth, or reduce the numbers, of food pathogens such as listeria monocytogenes and campylobacter jejuni in chicken products packaged under modified atmosphere (map), the effectiveness of protective cultures was evaluated in this study. leuconostoc pseudomesenteroides pck18 reduced the counts of l. monocytogenes by 1.22 log cfu/g in chicken burgers under map after 24 d. furthermore, a reduction of 1.16 log cfu/g in c. jejuni together with a delay in the growth of lactic acid bacte ... | 2013 | 23472035 |
| proteomic analysis of bifidobacterium longum subsp. infantis reveals the metabolic insight on consumption of prebiotics and host glycans. | bifidobacterium longum subsp. infantis is a common member of the intestinal microbiota in breast-fed infants and capable of metabolizing human milk oligosaccharides (hmo). to investigate the bacterial response to different prebiotics, we analyzed both cell wall associated and whole cell proteins in b. infantis. proteins were identified by lc-ms/ms followed by comparative proteomics to deduce the protein localization within the cell. enzymes involved in the metabolism of lactose, glucose, galacto ... | 2013 | 23469017 |
| fast screening of bifidobacterium longum sublethal stress conditions in a novel two-stage continuous culture strategy. | a central issue in the application of probiotics as food additives is their fastidious production and their sensitivity to many environmental stresses. the importance of inducible cell-protective mechanisms triggered by application of sublethal stresses for survival under stress conditions has been demonstrated. continuous cultures could be a suitable and more efficient method to test stress factors on one culture instead of several repeated batch cultures. in this study, the application of a tw ... | 2013 | 23443949 |
| a probiotics-containing biscuit modulates the intestinal microbiota in the elderly. | evaluation of the impact of a biscuit containing the probiotics bifidobacterium longum bar33 and lactobacillus helveticus bar13 on the intestinal microbiota in the elderly. | 2013 | 23364497 |
| development of antimicrobial synbiotics using potentially-probiotic faecal isolates of lactobacillus fermentum and bifidobacterium longum. | the aims of the present study were to investigate in vitro the antimicrobial activity of lactobacillus fermentum and bifidobacterium longum, isolated from faeces of healthy elderly individuals, against enterohaemorrhagic escherichia coli (e. coli o157:h7) and enteropathogenic e. coli (e. coli o86), to determine the capability of the selected strains to tolerate acid and bile in vitro, to select suitable carbohydrates in order to enhance the growth and maximise antimicrobial activity of the putat ... | 2013 | 23354005 |
| thymosin alpha-1-transformed bifidobacterium promotes t cell proliferation and maturation in mice by oral administration. | thymosin alpha-1 (tα1) has been used as an immune potentiator for treatment of immune deficiency diseases by injection administration. however, injection is inconvenient and may cause many side effects. in order to improve the administration convenience of tα1, a human tα1 gene transformed bifidobacterium longum (bl-tα1) was prepared and its effects on mice immunity by oral administration were investigated. the balb/c mice were treated with bl-tα1, which was pre-induced with 0.2% l-arabinose, ev ... | 2013 | 23352493 |
| oral immunization of mice using bifidobacterium longum expressing vp1 protein from enterovirus 71. | bifidobacterium longum is an attractive candidate for delivering biologically active proteins by the mucosal route due to its non-pathogenic and colonizing properties. enterovirus 71 (ev71) has aroused widespread attention recently due to several epidemics, and great attention should be paid to the fact that there are currently no effective antiviral drugs or vaccines against ev71 infection. in this report, we described a recombinant b. longum that could be used to develop an oral vaccine agains ... | 2013 | 23275129 |
| the pyre gene as a bidirectional selection marker in bifidobacterium longum 105-a. | we constructed a deletion mutant of the pyre gene in bifidobacterium longum 105-a. a pyre knockout cassette was cloned into pkkt427, a bifidobacterium-escherichia coli shuttle vector, and then introduced into b. longum 105-a by electroporation. the transformants were propagated and spread onto mrs plates containing 5-fluoroorotic acid (5-foa) and uracil. 5-foa-resistant mutants were obtained at a frequency of 4.7 × 10(-5) integrations per cell. to perform pyre gene complementation, the pyre gene ... | 2013 | 24936363 |
| effects of enteric-coated lactoferrin tablets containing lactobacillus brevis subsp. coagulans on fecal properties, defecation frequency and intestinal microbiota of japanese women with a tendency for constipation: a randomized placebo-controlled crossover study. | the effects of oral administration of enteric-coated tablets containing lactoferrin (lf; 100 mg/tablet) and heat-killed lactobacillus brevis subsp. coagulans frem bp-4693 (lb; 6×10(9) bacteria/tablet) on fecal properties were examined in 32 japanese women (20-60 years of age) with a tendency for constipation (defecation frequency at equal to or less than 10 times/2 weeks) by a double-blind placebo-controlled crossover design. a significant increase in defecation days per week was obserbed in the ... | 2013 | 24936358 |
| bifidobacterium infantis 35624 protects against salmonella-induced reductions in digestive enzyme activity in mice by attenuation of the host inflammatory response. | salmonella-induced damage to the small intestine may decrease the villi-associated enzyme activity, causing malabsorption of nutrients and diarrhea, and thus contribute to the symptoms of infection. the objective of this study was to determine the mechanism by which different doses and durations of salmonella infection and lipopolysaccharide (lps) affect brush border enzyme activity in the mouse, and to determine if the probiotic bifidobacterium longum subspecies infantis 35624 could attenuate t ... | 2012 | 23238232 |
| transcriptome analysis and physiology of bifidobacterium longum ncc2705 cells under continuous culture conditions. | a central issue in the use of probiotics in food and food supplements is their sensitivity to many environmental stress factors. the resistance of probiotic cells to lethal stress can be improved by application of homologous or heterologous sub-lethal stress during culture. this screening procedure is generally performed using batch cultures. continuous cultures could be a suitable and more efficient method to test different stress factors on one culture instead of repeating several batch cultur ... | 2012 | 23234728 |
| sequence analysis of plasmid psp02 from bifidobacterium longum m62 and construction of psp02-derived cloning vectors. | replicons from bifidobacteria species are required for the construction of general- and special-purpose vectors that would allow the undertaking of molecular studies of these bacteria. in this work, psp02, a cryptic plasmid from bifidobacterium longum m62, was cloned, sequenced and characterized. psp02 was found to consist of 4896bp with four orfs coding for proteins over 50 amino acids long. among the deduced protein sequences only a replicase (repa) and a mobilization-like protein (moba) showe ... | 2013 | 23228478 |
| utilization of galactooligosaccharides by bifidobacterium longum subsp. infantis isolates. | prebiotics are non-digestible substrates that stimulate the growth of beneficial microbial populations in the intestine, especially bifidobacterium species. among them, fructo- and galacto-oligosaccharides are commonly used in the food industry, especially as a supplement for infant formulas. mechanistic details on the enrichment of bifidobacteria by these prebiotics are important to understand the effects of these dietary interventions. in this study the consumption of galactooligosaccharides w ... | 2013 | 23200660 |
| developing an efficient and reproducible conjugation-based gene transfer system for bifidobacteria. | bifidobacteria are widely used as probiotics and have attracted increasing research interest worldwide. however, molecular techniques are still very scarce mainly due to the low efficiencies and strain-specific electroporation protocols that have been developed. bacterial conjugation enables the transfer of genetic material among a relatively wide range of organisms and with virtually no size limitation. a conjugation protocol was developed based on the rp4 conjugative machinery in the escherich ... | 2013 | 23197173 |
| clinical effects of probiotic bifidobacterium longum bb536 on immune function and intestinal microbiota in elderly patients receiving enteral tube feeding. | immune system function declines with age. we evaluated the effects of supplementation with the probiotic bifidobacterium longum bb536 on immune function and intestinal microbiota in the elderly. | 2013 | 23192454 |
| combination of ononis hirta and bifidobacterium longum decreases syngeneic mouse mammary tumor burden and enhances immune response. | the resistance of solid tumors to conventional therapies has prompted the need for alternative therapies. | 2012 | 23174725 |
| application of bifidobacterial phytases in infant cereals: effect on phytate contents and mineral dialyzability. | phytase activity was recently described in probiotic bifidobacterial strains, opening the possibilities for their use in foods, due to the generally regarded as safe/qualified presumption of safety status of these bacteria. two raw materials for infant cereals (multicereal and gluten-free) were examined by measuring the myo-inositol phosphates content and the in vitro ca, fe, and zn availability after a dephytinization process with purified phytases from bifidobacterium longum spp. infantis and ... | 2012 | 23151205 |
| maternal probiotic supplementation during pregnancy and breast-feeding reduces the risk of eczema in the infant. | probiotics have shown promising potential in reducing the risk of eczema in infants. optimal probiotic intervention regimen remains to be determined. | 2012 | 23083673 |
| attenuation of post-myocardial infarction depression in rats by n-3 fatty acids or probiotics starting after the onset of reperfusion. | proinflammatory cytokines play a central role in depression-like behaviour and apoptosis in the limbic system after myocardial infarction (mi). a pufa n-3 diet or the combination of lactobacillus helveticus r0052 and bifidobacterium longum r0175 probiotics, when given before the ischaemic period, reduce circulating proinflammatory cytokines as well as apoptosis in the limbic system. the present study was designed to determine if the same nutritional interventions maintain their beneficial effect ... | 2013 | 23068715 |
| image analyzing method to evaluate in situ bioluminescence from an obligate anaerobe cultivated under various dissolved oxygen concentrations. | an image analyzing method was developed to evaluate in situ bioluminescence expression, without exposing the culture sample to the ambient oxygen atmosphere. using this method, we investigated the effect of dissolved oxygen concentration on bioluminescence from an obligate anaerobe bifidobacterium longum expressing bacterial luciferase which catalyzes an oxygen-requiring bioluminescent reaction. | 2013 | 23040354 |
| the principal fucosylated oligosaccharides of human milk exhibit prebiotic properties on cultured infant microbiota. | breast-fed infant microbiota is typically rich in bifidobacteria. herein, major human milk oligosaccharides (hmos) are assessed for their ability to promote the growth of bifidobacteria and to acidify their environment, key features of prebiotics. during in vitro anaerobic fermentation of infant microbiota, supplementation by hmos significantly decreased the ph even greater than supplementation by fructooligosaccharide (fos), a prebiotic positive control. hmos elevated lactate concentrations, in ... | 2013 | 23028202 |
| oral administration of bifidobacterium longum cect 7347 modulates jejunal proteome in an in vivo gliadin-induced enteropathy animal model. | celiac disease is an immune-mediated disorder triggered by gluten proteins of wheat (gliadins) and other cereals. gliadin-mediated effects on weanling animals, sensitized or not with interferon (ifn)-γ, were investigated. also, the influence of the co-administration of bifidobacterium longum cect 7347 was studied together with changes in the proteome of jejunal sections, using 2de and malditof-tof peptide fingerprinting. findings were compared to results for control animal groups. in the princip ... | 2012 | 23023000 |
| bifidobacterium spp. influences the production of autoinducer-2 and biofilm formation by escherichia coli o157:h7. | the effect of bifidobacterium spp. on the production of quorum-sensing (qs) signals and biofilm formation by enterohemorrhagic escherichia coli (ehec) o157:h7 was investigated. in an ai-2 bioassay, cell extracts of bifidobacterium longum atcc 15707 resulted in a 98-fold reduction in ai-2 activity in ehec o157:h7 as well as in the vibrio harveyi reporter strain, even though they did not inhibit the growth of ehec o157:h7. in addition, they resulted in a 36% reduction in biofilm formation by the o ... | 2012 | 23010308 |
| cultivable and pyrosequenced fecal microflora in centenarians and young subjects. | the aim of this study was to characterize the composition of the intestinal microbiota in healthy centenarians in comparison with younger adults, considering both quantitative and qualitative aspects of gut community structure. | 2012 | 22955365 |
| active hexose-correlated compound and bifidobacterium longum bb536 exert symbiotic effects in experimental colitis. | active hexose-correlated compound (ahcc) is a commercial extract obtained from basidiomycetes under controlled conditions, yielding a 74 % content in oligosaccharides, especially α-glucans. ahcc has a number of therapeutic effects, including intestinal anti-inflammatory activity. bifidobacterium longum bb536 is a probiotic with potential health-promoting effect at the gut level. the purpose of the present study was to evaluate the possibility of synergism between ahcc, which is believed to act a ... | 2013 | 22941198 |
| emulsifying, rheological and physicochemical properties of exopolysaccharide produced by bifidobacterium longum subsp. infantis ccug 52486 and bifidobacterium infantis ncimb 702205. | the rheological, emulsification and certain physicochemical properties of purified exopolysaccharides (eps) of bifidobacterium longum subsp. infantis ccug 52486 and bifidobacterium infantis ncimb 702205 were studied and compared with those of guar gum and xanthan gum. the two strains were grown in skim milk supplemented with 1.5% (w/v) casein hydrolysate at 37 °c for 24h; they both produced heteropolysaccharides with different molecular mass and composition. the carbohydrate content of both poly ... | 2012 | 24751074 |
| d-tagatose production in the presence of borate by resting lactococcus lactis cells harboring bifidobacterium longum l-arabinose isomerase. | bifidobacterium longum nrrl b-41409 l-arabinose isomerase (l-ai) was overexpressed in lactococcus lactis using a phosphate depletion inducible expression system. the resting l. lactis cells harboring the b. longum l-ai were used for production of d-tagatose from d-galactose in the presence of borate buffer. multivariable analysis suggested that high ph, temperature and borate concentration favoured the conversion of d-galactose to d-tagatose. almost quantitative conversion (92 %) was achieved at ... | 2013 | 22903573 |
| evidence of immunomodulatory effects of a novel probiotic, bifidobacterium longum bv. infantis ccug 52486. | bifidobacterium longum bv. infantis ccug 52486 was originally isolated from healthy elderly subjects and demonstrated to have particular ecological fitness and anti-pathogenic effects. bifidobacteria are commonly associated with immuno-modulatory properties, especially in older people, but this strain has not been investigated for effects on immune function. this study aimed to explore the immunomodulatory effects of this novel probiotic, compared with three commercial strains, b. longum sp 07/3 ... | 2012 | 22882710 |
| hypocholesterolaemic effect of yoghurt containing bifidobacterium pseudocatenulatum g4 or bifidobacterium longum bb536. | the effect of a yoghurt supplement containing bifidobacterium pseudocatenulatum g4 or bifidobacterium longum bb536 on plasma lipids, lipid peroxidation and the faecal excretion of bile acids was examined in rats fed a cholesterol-enriched diet. after 8 weeks, the rats in the positive control (pc) group who were fed the cholesterol-enriched diet showed significant increases in plasma total cholesterol (tc), low-density lipoprotein (ldl) cholesterol, and malondialdehyde (mda). however, groups fed ... | 2012 | 22868099 |
| oral administration of bifidobacterium longum in a gastro-resistant seamless capsule decreases serum phosphate levels in patients receiving haemodialysis. | | 2012 | 25874106 |
| bifidobacterium longum l-arabinose isomerase--overexpression in lactococcus lactis, purification, and characterization. | bifidobacterium longum nrrl b-41409 l-arabinose isomerase (l-ai) was cloned and overexpressed in lactococcus lactis using a phosphate-depletion-inducible expression system. the purified b. longum l-ai was characterized using d-galactose and l-arabinose as the substrates. the enzyme was active and stable at acidic ph with an optimum at ph 6.0-6.5. the enzyme showed the highest activity at 55 °c during a 20-min incubation at ph 6.5. the k(m) value was 120 mm for l-arabinose and 590 mm for d-galact ... | 2012 | 22763951 |
| probiotic cheese attenuates exercise-induced immune suppression in wistar rats. | intense physical activity results in a substantial volume of stress and hence a significant probability of immunosuppression in athletes, with milk proteins being, perhaps, the most recommended protein supplements. consumption of a probiotic cheese can attenuate immune suppression induced by exhausting exercise in rats. a popular brazilian fresh cheese (minas frescal cheese) containing lactobacillus acidophilus la14 and bifidobacterium longum bl05 was fed for 2wk to adult wistar rats, which then ... | 2012 | 22720913 |
| encapsulation of probiotic bacteria in lamb rennet paste: effects on the quality of pecorino cheese. | lamb rennet pastes containing encapsulated lactobacillus acidophilus and a mix of bifidobacterium longum and bifidobacterium lactis were produced for pecorino cheese manufacture from gentile di puglia ewe milk. cheeses were denoted as rp cheese when made with traditional rennet paste, rp-l cheese when made with rennet paste containing l. acidophilus culture, and rp-b cheese when made with rennet paste containing a mix of b. lactis and b. longum. biochemical features of pecorino cheese were studi ... | 2012 | 22720908 |
| immunomodulatory effects of feeding with bifidobacterium longum on allergen-induced lung inflammation in the mouse. | the intestinal microbiota has important effects on host immune responses and feeding with certain commensal organisms has anti-inflammatory effects in a variety of diseases, including experimental asthma. the aim of the current study was to examine how robust the effects of feeding with the commensal strain, bifidobacterium longum (bif) were on the pulmonary responses to allergen sensitization and challenge. | 2012 | 22705947 |
| effects of bile salt deconjugation by probiotic strains on the survival of antibiotic-resistant foodborne pathogens under simulated gastric conditions. | this study was designed to evaluate the effects of bile acid deconjugation by probiotic strains on the antibiotic susceptibility of antibiotic-sensitive and multiple antibiotic-resistant salmonella typhimurium and staphylococcus aureus. eight probiotic strains, bifidobacterium longum b6, lactobacillus acidophilus adh, lactobacillus brevis kacc 10553, lactobacillus casei kacc 12413, lactobacillus paracasei atcc 25598, lactobacillus rhamnosus gg, leuconostoc mesenteroides kacc 12312, and pediococc ... | 2012 | 22691477 |
| galacto-oligosaccharides have prebiotic activity in a dynamic in vitro colon model using a (13)c-labeling technique. | galacto-oligosaccharides (gos) are considered to be prebiotic, although the contribution of specific members of the microbiota to gos fermentation and the exact microbial metabolites that are produced upon gos fermentation are largely unknown. we aimed to determine this using uniformly (13)c-labeled gos. the normal (control) medium and unlabeled or (13)c-labeled gos was added to a dynamic, validated, in vitro model of the large-intestine containing an adult-type microbiota. liquid-chromatography ... | 2012 | 22623395 |
| antagonistic action of lactobacilli and bifidobacteria in relation to staphylococcus aureus and their influence on the immune response in cases of intravaginal staphylococcosis in mice. | the antibacterial activity of lactobacillus casei imv b-7280, lact. acidophilus imv b-7279, bifidobacterium longum vk1, and b. bifidum vk2 strains or their various compositions in relation to staphylococcus aureus in vitro and on models of experimental intravaginal staphylococcosis of mice was determined. it was found that under the influence of these strains and their various compositions, the in vitro growth of staph. aureus was inhibited, and the number of colonies of staph. aureus plated fro ... | 2012 | 22611454 |
| a molecular basis for bifidobacterial enrichment in the infant gastrointestinal tract. | bifidobacteria are commonly used as probiotics in dairy foods. select bifidobacterial species are also early colonizers of the breast-fed infant colon; however, the mechanism for this enrichment is unclear. we previously showed that bifidobacterium longum subsp. infantis is a prototypical bifidobacterial species that can readily utilize human milk oligosaccharides as the sole carbon source. ms-based glycoprofiling has revealed that numerous b. infantis strains preferentially consume small mass o ... | 2012 | 22585920 |
| development of a double-crossover markerless gene deletion system in bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. | functional analysis of bifidobacterium genes is essential for understanding host-bifidobacterium interactions with beneficial effects on human health; however, the lack of an effective targeted gene inactivation system in bifidobacteria has prevented the development of functional genomics in this bacterium. here, we report the development of a markerless gene deletion system involving a double crossover in bifidobacterium longum. incompatible plasmid vectors were used to facilitate a second cros ... | 2012 | 22582061 |
| novel phytases from bifidobacterium pseudocatenulatum atcc 27919 and bifidobacterium longum subsp. infantis atcc 15697. | two novel phytases have been characterized from bifidobacterium pseudocatenulatum and bifidobacterium longum subsp. infantis. the enzymes belong to a new subclass within the histidine acid phytases, are highly specific for the hydrolysis of phytate, and render myo-inositol triphosphate as the final hydrolysis product. they represent the first phytases characterized from this group of probiotic microorganisms, opening the possibilities for their use in the processing of high-phytate-content foods ... | 2012 | 22582052 |
| an in vitro evaluation of the effect of probiotics and prebiotics on the metabolic profile of human microbiota. | in the current study, batch culture fermentations on fecal samples of 3 healthy individuals were performed to assess the effect of the addition of prebiotics (fos), probiotics (bifidobacterium longum bar33 and lactobacillus helveticus bar13) and synbiotics (b. longum bar33 + l. helveticus bar13 + fos) on the fecal metabolic profiles. a total of 84 different metabolites belonging to the families of sulfur compounds, nitrogen compounds, aldehydes, ketones, esters, alcohols, phenols, organic acids, ... | 2012 | 22579985 |
| release and utilization of n-acetyl-d-glucosamine from human milk oligosaccharides by bifidobacterium longum subsp. infantis. | human milk contains high amounts of complex oligosaccharides, which can be utilized especially by bifidobacterium species in the infant gut as a carbon and energy source. n-acetyl-d-glucosamine is a building block of these oligosaccharides, and molecular details on the release and utilization of this monosaccharide are not fully understood. in this work we have studied some of the enzymatic properties of three n-acetyl-β-d-hexosaminidases encoded by the genome of the intestinal isolate bifidobac ... | 2012 | 22579845 |
| in vitro utilization of gold and green kiwifruit oligosaccharides by human gut microbial populations. | we examined the effects of whole kiwifruit on gut microbiota using an in vitro batch model of gastric-ileal digestion and colonic fermentation. faecal fermentations of gold and green kiwifruit, inulin and water (control) digests were performed for up to 48 h. as compared to the control, gold and green kiwifruit increased bifidobacterium spp. by 0.9 and 0.8 log(10) cfu/ml, respectively (p < 0.001), and the bacteroides-prevotella-porphyromonas group by 0.4 and 0.5 log(10) cfu/ml, respectively. inu ... | 2012 | 22576129 |
| supplementation of broccoli or bifidobacterium longum-fermented broccoli suppresses serum lipid peroxidation and osteoclast differentiation on alveolar bone surface in rats fed a high-cholesterol diet. | high-cholesterol diet enhances osteoclastic activity on alveolar bone by increasing serum lipid peroxidation. we hypothesized that supplementation with dietary antioxidants, such as found in broccoli and its fermented products, might suppress increases in serum lipid peroxidation, contributing to the inhibition of osteoclastic activity after high-cholesterol diet intake. the purpose of the present study was to investigate the effects of broccoli and fermented broccoli consumption on serum lipid ... | 2012 | 22575044 |
| yacon flour and bifidobacterium longum modulate bone health in rats. | yacon flour has been considered a food with prebiotic potential because of the high levels of fructooligosaccharides, which allows for its use in formulating synbiotic foods. the purpose of this study was to evaluate the effect of yacon flour and probiotic (bifidobacterium longum) on the modulation of variables related to bone health. thirty-two wistar rats were divided into 4 groups: control, yacon flour, diet+b. longum, and yacon flour+b. longum. after euthanasia, the bones were removed for an ... | 2012 | 22510044 |
| differential expression of virulence and stress fitness genes during interaction between listeria monocytogenes and bifidobacterium longum. | bifidobacterium is well known to have an inhibitory effect on the survival, growth, and proliferation of various foodborne pathogens, but the mechanism of the molecular action of b. longum in blocking the invasion of listeria monocytogenes is not yet well defined. in the present study, following rna extraction and cdna synthesis, differential expression of virulence and stress fitness genes in l. monocytogenes and b. longum was determined by real-time pcr. the results indicate that l. monocytoge ... | 2012 | 22484935 |
| metabolism of ginsenoside rb1 by human intestinal microflora and cloning of its metabolizing β-d-glucosidase from bifidobacterium longum h-1. | to understand the role of intestinal microflora in expressing the pharmacological effect of ginsenoside rb1, the metabolic activity of ginsenoside rb1 by 148 fecal specimens was measured and its metabolizing β-glucosidase was cloned. the average activities for p-nitrophenyl-β-d-glucopyranoside and ginsenoside rb1 were 0.097±0.059 μmol/min/mg and 0.311±0.118 pmol/min/mg, respectively. these enzyme activities were not different between male and female, or between ages. a gene encoding β-d-glucosid ... | 2012 | 22466563 |
| comparative analysis of mycobacterium and related actinomycetes yields insight into the evolution of mycobacterium tuberculosis pathogenesis. | the sequence of the pathogen mycobacterium tuberculosis (mtb) strain h37rv has been available for over a decade, but the biology of the pathogen remains poorly understood. genome sequences from other mtb strains and closely related bacteria present an opportunity to apply the power of comparative genomics to understand the evolution of mtb pathogenesis. we conducted a comparative analysis using 31 genomes from the tuberculosis database (tbdb.org), including 8 strains of mtb and m. bovis, 11 addi ... | 2012 | 22452820 |
| cell viability and immunostimulating and protective capacities of bifidobacterium longum 51a are differentially affected by technological variables in fermented milks. | to investigate the cell viability of bifidobacterium longum 5(1a) in fermented milks and to study its immunostimulating and protective capacity against salmonella enterica ssp. enterica serovar typhimurium infection in mice. | 2012 | 22394272 |
| bifidobacteria isolated from infants and cultured on human milk oligosaccharides affect intestinal epithelial function. | human milk oligosaccharides (hmos) are the third most abundant component of breast milk. our laboratory has previously revealed gene clusters specifically linked to hmo metabolism in selected bifidobacteria isolated from fecal samples of infants. our objective was to test the hypothesis that growth of selected bifidobacteria on hmo stimulates the intestinal epithelium. | 2012 | 22383026 |
| effect of water activity and protective solutes on growth and subsequent survival to air-drying of lactobacillus and bifidobacterium cultures. | probiotic cultures of lactobacillus plantarum, lactobacillus rhamnosus, bifidobacterium longum, lactobacillus casei and lactobacillus acidophilus were grown in media having water activities (a (w)) adjusted between 0.99 and 0.94 with nacl or with a mixture of glycerol and sucrose in order to find conditions of osmotic stress which would still allow for good growth. cultures grown at a (w) = 0.96 or 0.99 were then recovered by centrifugation, added to a sucrose-phosphate medium and air-dried. in ... | 2012 | 22350318 |
| bifidobacterium longum cect 7347 modulates immune responses in a gliadin-induced enteropathy animal model. | coeliac disease (cd) is an autoimmune disorder triggered by gluten proteins (gliadin) that involves innate and adaptive immunity. in this study, we hypothesise that the administration of bifidobacterium longum cect 7347, previously selected for reducing gliadin immunotoxic effects in vitro, could exert protective effects in an animal model of gliadin-induced enteropathy. the effects of this bacterium were evaluated in newborn rats fed gliadin alone or sensitised with interferon (ifn)-γ and fed g ... | 2012 | 22348021 |
| safety assessment of bifidobacterium longum jdm301 based on complete genome sequences. | to assess the safety of bifidobacterium longum (b. longum) jdm301 based on complete genome sequences. | 2012 | 22346255 |
| efficient one-pot multienzyme synthesis of udp-sugars using a promiscuous udp-sugar pyrophosphorylase from bifidobacterium longum (blusp). | a promiscuous udp-sugar pyrophosphorylase (blusp) was cloned from bifidobacterium longum strain atcc55813 and used efficiently with a pasteurella multocida inorganic pyrophosphatase (pmppa) with or without a monosaccharide 1-kinase for one-pot multienzyme synthesis of udp-galactose, udp-glucose, udp-mannose, and their derivatives. further chemical diversification of a udp-mannose derivative resulted in the formation of udp-n-acetylmannosamine. | 2012 | 22306833 |
| removal of cholera toxin from aqueous solution by probiotic bacteria. | cholera remains a serious health problem, especially in developing countries where basic hygiene standards are not met. the symptoms of cholera are caused by cholera toxin, an enterotoxin, which is produced by the bacterium vibrio cholerae. we have recently shown that human probiotic bacteria are capable of removing cyanobacterial toxins from aqueous solutions. in the present study we investigate the ability of the human probiotic bacteria, lactobacillus rhamnosus strain gg (atcc 53103) and bifi ... | 2012 | 24281668 |
| investigation of the factors influencing the survival of bifidobacterium longum in model acidic solutions and fruit juices. | the survival of bifidobacterium longum ncimb 8809 was studied during refrigerated storage for 6weeks in model solutions, based on which a mathematical model was constructed describing cell survival as a function of ph, citric acid, protein and dietary fibre. a central composite design (ccd) was developed studying the influence of four factors at three levels, i.e., ph (3.2-4), citric acid (2-15g/l), protein (0-10g/l), and dietary fibre (0-8g/l). in total, 31 experimental runs were carried out. a ... | 2011 | 25212334 |
| selenium-bifidobacterium longum as a delivery system of endostatin for inhibition of pathogenic bacteria and selective regression of solid tumor. | bifidobacterium longum (b. longum) as a delivery system for endostatin was shown to have definite antitumor effects. moreover, it was found that the enrichment of selenium was able to enhance the immunity of mice. in order to further evaluate the safety and efficacy of b. longum carrying pbv22210-endostatin (b. longum-en) enriched with selenium (se-b. longum-en), we determined the biochemical characteristics of se-b. longum-en. we then investigated its effect on macrophage activity, as well as i ... | 2010 | 23136605 |
| determination of differentially expressed genes involved in arabinoxylan degradation by bifidobacterium longum ncc2705 using real-time rt-pcr. | real-time quantitative pcr (qrt-pcr) can be used to monitor specific catabolic activity by gene transcriptional analysis of bacterial cultures. this methodology has been applied to determine if the differential expression of genes putatively involved in arabinoxylan degradation by bifidobacterium longum ncc2705 could be associated to the consumption of this prebiotic. three genes putatively encoding arabinofuranosidases (abfi, abfa, and abfb) and one putatively encoding endoxylanase (xynd) were ... | 2009 | 26783166 |
| a nutritious medida (sudanese cereal thin porridge) prepared by fermenting malted brown rice flour with bifidobacterium longum bb 536. | the nutritive value of spontaneously fermented brown rice flour medida, a sudanese cereal thin porridge, is low. this study was carried out to improve the nutritional quality of medida. the flour was soaked and malted at 30◦c to optimise the protein content. flour malted for two days had the highest protein content. skim milk was added to the malted brown rice flour medida and fermented using bifidobacterium longum bb 536. maximum count of b. longum bb 536 up to 9 log cfu/ ml was attained at 4.6 ... | 2004 | 22691739 |
| probiotics and colon cancer prevention. | this review examines some of the evidence regarding probiotic bacteria as agents to reduce the risk of colon cancer in humans. while some of the evidence using rodent models of colon cancer is convincing for a reduction in cancer incidence and burden with the introduction orally of such bacteria as bifidobacterium longum, lactobacillus acidophilus and gg, convincing evidence in humans is more difficult to find. it consists of epidemiological studies or marker intervention studies using faecal en ... | 1996 | 24394467 |