| naturally occurring entomopathogenic fungi infecting stored grain insect species in punjab, pakistan. | the occurrence of entomopathogenic fungi isolated from stored grain insect pests sampled from various geographical regions of punjab, pakistan, was investigated. in total, 25,720 insects from six different species were evaluated, and 195 isolates from 24 different fungal species were recovered. these included the ascomycetes beauveria bassiana sensu lato (balsamo) vuillemin (hypocreales: clavicipitaceae), metarhizium anisopliae sensu lato (metschnikoff) sorokin (hypocreales: clavicipitaceae), pu ... | 2014 | 25480970 |
| responsiveness of entomopathogenic fungi to menadione-induced oxidative stress. | entomopathogenic fungi are predisposed to ros induced by heat and uv-a radiation when outside the insect host. when inside the host, they are subject to phagocytic cells that generate ros to eliminate invading pathogens. the oxidative stress tolerance of the entomopathogenic fungi aschersonia aleyrodis (arsef 430 and 10276), aschersonia placenta (arsef 7637), beauveria bassiana (arsef 252), isaria fumosorosea (arsef 3889), lecanicillium aphanocladii (arsef 6433), metarhizium acridum (arsef 324), ... | 2014 | 25457946 |
| til-type protease inhibitors may be used as targeted resistance factors to enhance silkworm defenses against invasive fungi. | entomopathogenic fungi penetrate the insect cuticle using their abundant hydrolases. these hydrolases, which include cuticle-degrading proteases and chitinases, are important virulence factors. our recent findings suggest that many serine protease inhibitors, especially til-type protease inhibitors, are involved in insect resistance to pathogenic microorganisms. to clarify the molecular mechanism underlying this resistance to entomopathogenic fungi and identify novel genes to improve the silkwor ... | 2015 | 25453359 |
| mortality risk from entomopathogenic fungi affects oviposition behavior in the parasitoid wasp trybliographa rapae. | biological control of pests in agroecosystems could be enhanced by combining multiple natural enemies. however, this approach might also compromise the control efficacy through intraguild predation (igp) among the natural enemies. parasitoids may be able to avoid the risk of unidirectional igp posed by entomopathogenic fungi through selective oviposition behavior during host foraging. trybliographa rapae is a larval parasitoid of the cabbage root fly, delia radicum. here we evaluated the suscept ... | 2015 | 25446037 |
| rna sequencing analysis identifies the metabolic and developmental genes regulated by bbsnf1 during conidiation of the entomopathogenic fungus beauveria bassiana. | conidiation promotes fungal dispersal and survival in the environment, and is a determinant for the biocontrol potential of beauveria bassiana. the snf1/ampk protein kinases function as an important regulator of fungal development and energy metabolism, and play a crucial role in conidiation of the filamentous fungi. in previous study, it has been established that the b. bassiana homolog (bbsnf1) controls conidial production. this study showed that the δbbsnf1 mutants displayed a delayed develop ... | 2015 | 25417093 |
| complete dsrna sequence of a novel victorivirus isolated from the pear stem wart fungus botryosphaeria dothidea. | botryosphaeria dothidea is a pathogen that causes many important diseases of deciduous fruit trees worldwide. in this study, a double-stranded (ds) rna of 5322 base pairs (bp) in length from strain gy25 of b. dothidea was isolated and sequenced. the dsrna potentially contained two overlapping open reading frames (orfs). the predicted gene product of orf1 had the highest amino acid sequence similarity, 37 % (e-value, 8e(-101)), to the capsid protein of rosellinia necatrix victorivirus 1 (rnv1), a ... | 2015 | 25416855 |
| a novel mitochondrial membrane protein, ohmm, limits fungal oxidative stress resistance and virulence in the insect fungal pathogen beauveria bassiana. | the hog1 mitogen-activated protein (map) kinase regulates environmental stress responses and virulence in the entomopathogenic fungus beauveria bassiana. to further characterize this pathway, we constructed a subtraction library enriched for genes regulated by hog1. one targeted gene, encoding a novel membrane protein, ohmm (oxidative homeostasis membrane-protein-mitochondria), was uniquely identified as being downregulated in the δhog1 background during growth under non-stress and osmotic stres ... | 2015 | 25403093 |
| selectivity of metarhizium anisopliae and beauveria bassiana (hypocreales: clavicipitaceae) on adults of cotesia flavipes (hymenoptera: braconidae). | understanding mortality patterns and interactions between entomopathogenic fungi and parasitoids is important to improve insect biological control programs. the aim of this study was to evaluate the effect of metarhizium anisopliae (metschnikoff, 1879) sorokin, 1833 and beauveria bassiana (balsamo) vuillemin, 1912 (hypocreales: clavicipitaceae) on adults of cotesia flavipes (cameron, 1891) (hymenoptera: braconidae) with biological insecticides biometha wp plus (m. anisopliae), biovéria g (b. bas ... | 2014 | 25403080 |
| selection of beauveria isolates pathogenic to adults of nilaparvata lugens. | the brown planthopper, nilaparvata lugens stål (hemiptera: delphacidae), is a destructive invasive pest and has become one of the most economically-important rice pests in china. effective control measures are desperately needed. entomopathogenic fungi, such as beauveria bassiana (balsamo-crivelli) vuillemin (hypocreales: clavicipitaceae) and b. brongniartii (saccardo), have shown great potential for the management of some sucking pest species. in this study, to explore alternative strategies fo ... | 2014 | 25373179 |
| functional analysis of insect molting fluid proteins on the protection and regulation of ecdysis. | molting fluid accumulates between the old and new cuticles during periodical ecdysis in ecdysozoa. natural defects in insect ecdysis are frequently associated with melanization (an immunity response) occurring primarily in molting fluids, suggesting that molting fluid may impact immunity as well as affect ecdysis. to address this hypothesis, proteomic analysis of molting fluids from bombyx mori during three different types of ecdysis was performed. many proteins were newly identified, including ... | 2014 | 25368323 |
| susceptibility of adults of the cerambycid beetle hedypathes betulinus to the entomopathogenic fungi beauveria bassiana, metarhizium anisopliae, and purpureocillium lilacinum. | the cerambycid beetle hedypathes betulinus (klug) (coleoptera: cerambycidae) causes severe damage to yerba mate plants (ilex paraguariensis (st. hilaire) (aquifoliales: aquifoliaceae)), which results in large losses of production. in this study, the pathogenicity of entomopathogenic fungi of the species beauveria bassiana (balsamo-crivelli) vuillemin (hypocreales: cordycipitaceae), metarhizium anisopliae sensu lato (metschnikoff) sorokin (hypocreales: clavicipitaceae), and purpureocillium lilaci ... | 2014 | 25368071 |
| a novel bioassay to evaluate the potential of beauveria bassiana strain ni8 and the insect growth regulator novaluron against lygus lineolaris on a non-autoclaved solid artificial diet. | a non-autoclaved solid diet was used to evaluate the entomopathogenic fungus beauveria bassiana (balsamo) vuillemin (hypocreales: clavicipitaceae) strain ni8 and the insect growth regulator novaluron (diamond® 0.83ec insecticide) for control of the tarnished plant bug, lygus lineolaris (palisot de beauvois) (hemiptera: miridae). the diet was composed of toasted wheat germ, ground lima bean meal, soy flour, yolk of chicken eggs, inhibitor, and agar. it was prepared in one step by blending the ing ... | 2014 | 25368059 |
| a case of beauveria bassiana keratitis confirmed by internal transcribed spacer and lsu rdna d1-d2 sequencing. | we describe a case of fungal keratitis due to beauveria bassiana in a farmer with fuchs' dystrophy, treated with amphotericin b. surgery with penetrating keratoplasty was necessary to resolve the lesions. susceptibility testing and molecular sequencing permitted the identification and treatment of this rare aetiological agent of invasive fungal disease. | 2014 | 25356350 |
| physiological changes in rhipicephalus microplus (acari: ixodidae) experimentally infected with entomopathogenic fungi. | carbohydrate metabolism plays an important role in the physiology and maintenance of energy stores within living organisms. however, when organisms are exposed to adverse physiological conditions, such as during pathogenic infection, these organisms begin to use alternative substrates (proteins and lipids) for energy production. this paper studied the carbohydrate metabolism of rhipicephalus microplus after infection with beauveria bassiana and metarhizium anisopliae. the parameters evaluated we ... | 2015 | 25346195 |
| effects of endophyte colonization of vicia faba (fabaceae) plants on the life-history of leafminer parasitoids phaedrotoma scabriventris (hymenoptera: braconidae) and diglyphus isaea (hymenoptera: eulophidae). | effects of the fungal endophytes beauveria bassiana (isolates icipe 279, g1lu3, s4su1) and hypocrea lixii (isolate f3st1) on the life-history of phaedrotoma scabriventris and diglyphus isaea, parasitoids of the pea leafminer liriomyza huidobrensis, were studied in the laboratory. parasitoids were allowed to parasitize 2(nd) and 3(rd) instar l. huidobrensis larvae reared on endophytically-inoculated faba bean, vicia faba. in the control, parasitoids were reared on non-inoculated host plants. para ... | 2014 | 25338084 |
| the role of three calcineurin subunits and a related transcription factor (crz1) in conidiation, multistress tolerance and virulence in beauveria bassiana. | the eukaryotic calcineurin (cn) pathway comprising catalytic a (cna) and regulatory b subunits (cnb) is crucial for many biological processes but functionally unexplored in entomopathogenic fungi. here, we characterise three cn subunits (cna1, cna2 and cnb) and a downstream cn-responsive zinc finger transcription factor (crz1) in beauveria bassiana. cn-mediated phosphatase activity decreased by 16-38 % in all deletion mutants compared with wild type. growth and conidiation were most defective in ... | 2015 | 25324131 |
| unveiling equal importance of two 14-3-3 proteins for morphogenesis, conidiation, stress tolerance and virulence of an insect pathogen. | two conserved 14-3-3 proteins orthologous to saccharomyces cerevisiae bmh1/2 are poorly understood in filamentous fungi. here we show that bmh1 and bmh2 contribute equally to the fundamental biology and physiology of beauveria bassiana by targeting many sets of proteins/enzymes. single bmh deletion caused similar upregulation of another. excellent knockdown (∼91%) expressions of bmh1 in δbmh2 and bmh2 in δbmh1 resulted in equally more severe multiphenotypic defects than the single deletions, inc ... | 2015 | 25315061 |
| oxidative stress in entomopathogenic fungi grown on insect-like hydrocarbons. | entomopathogenic fungi mostly attack their insect hosts by penetration through the cuticle. the outermost insect surface is covered by a lipid-rich layer, usually composed of very long chain hydrocarbons. these fungi are apt to grow on straight chain hydrocarbons (alkanes) as the sole carbon source. insect-like hydrocarbons are first hydroxylated by a microsomal p450 monooxygenase system, and then fully catabolized by peroxisomal β-oxidation reactions in beauveria bassiana. in this review, we wi ... | 2015 | 25274493 |
| distinct contributions of one fe- and two cu/zn-cofactored superoxide dismutases to antioxidation, uv tolerance and virulence of beauveria bassiana. | beauveria bassiana, a filamentous entomopathogen, has five distinct superoxide dismutases (sods), including cytosolic and mitochondrial mnsods (sod2/3) which have proved contributing primarily to intracelluar sod activity and additively to antioxidation and virulence. here we characterized cytosolic cu/znsod (sod1), mitochondrial fesod (sod4) and cell wall-anchored cu/znsod (sod5). the latter two are unexplored despite existence in many filamentous fungi, and their subcellular localization was w ... | 2015 | 25263710 |
| fungal keratitis caused by beauveria bassiana: drug and temperature sensitivity profiles: a case report. | beauveria bassiana is an entomopathogenic fungus and is a rare cause of keratitis. we present a case of fungal keratitis caused by b. bassiana that was diagnosed by in vivo confocal microscopy and in vitro corneal cultures. in addition, we determined the temperature- and drug-sensitivities of the isolated strain of b. bassiana. | 2014 | 25260975 |
| five vacuolar ca(2+) exchangers play different roles in calcineurin-dependent ca(2+)/mn(2+) tolerance, multistress responses and virulence of a filamentous entomopathogen. | multiple vcx1 (vacuolar calcium exchanger) paralogues exist in many filamentous fungi but are functionally unexplored unlike a single vcx1 ortholog well characterized in yeasts. here we show that five vcx1 paralogues (vcx1a-e) in beauveria bassiana are conditionally functional for intracellular ca(2+) homeostasis and contribute differentially to multistress tolerance and virulence in the filamentous entomopathogen. each vcx1 deletion drastically upregulated transcriptional expressions of four ot ... | 2014 | 25256588 |
| lethal effects of a mexican beauveria bassiana (balsamo) strain against meccus pallidipennis (stal). | the entomopathogenic fungus beauveria bassiana (balsamo 1835) vuillemin is an effective alternative control agent against some agricultural pests and biological vectors of important diseases such as chagas disease. in this work we studied an isolate of beauveria bassiana from of the town of san antonio rayón, puebla, mexico and its entomopathogenic effects on meccus pallidipennis (stal 1872). phylogenetic analysis using molecular comparison of the its and ef1α genes, showed that the resulting cl ... | 2014 | 25242941 |
| evaluation of insect associated and plant growth promoting fungi in the control of cabbage root flies. | delia radicum l. or cabbage maggot is an important pest for brassicaceous crops. there are currently no registered chemical control agents for its control in slovenia. fungal control agents for cabbage maggot were therefore sought among nine rhizosphere-compatible and plant growth-promoting, soil-adapted, and entomopathogenic species to cabbage maggots and were assayed in in vitro and soil laboratory bioassays. in the in vitro tests, the conidial suspensions were applied directly to cabbage magg ... | 2014 | 25195421 |
| a putative methyltransferase, mtra, contributes to development, spore viability, protein secretion and virulence in the entomopathogenic fungus beauveria bassiana. | the filamentous fungus, beauveria bassiana, is a ubiquitously distributed insect pathogen, currently used as an alternative to chemical pesticides for pest control. conidiospores are the means by which the fungus disseminates in the environment, and these cells also represent the infectious agent most commonly used in field applications. little, however, is known concerning the molecular basis for maintenance of spore viability, a critical feature for survival and persistence. here, we report on ... | 2014 | 25194143 |
| foster carers influence brood pathogen resistance in ants. | social organisms face a high risk of epidemics, and respond to this threat by combining efficient individual and collective defences against pathogens. an intriguing and little studied feature of social animals is that individual pathogen resistance may depend not only on genetic or maternal factors, but also on the social environment during development. here, we used a cross-fostering experiment to investigate whether the pathogen resistance of individual ant workers was shaped by their own col ... | 2014 | 25143036 |
| toxicity of naturally occurring bio-fly and chitosan compounds to control the mediterranean fruit fly ceratitis capitata (wiedemann). | the efficacy of five compounds of a biopolymer chitosan and bio-fly (beauveria bassiana fungus) as biopesticide was evaluated on ceratitis capitata under laboratory conditions. the inhibitory effects on acetylcholinesterase (ache) and adenosinetriphosphatase (atpase) as biochemical indicators were also determined in vivo. the results indicated that b. bassiana based bio-fly exhibited significant toxicity against c. capitata (lc50 = 3008 and 3126 mg/l after 48 h in females and males, respectively ... | 2015 | 25117027 |
| laboratory and field efficacy of entomopathogenic fungi for the management of the sweetpotato weevil, cylas formicarius (coleoptera: brentidae). | the sweetpotato weevil, cylas formicarius (f.) (coleoptera: brentidae), is one of the most important pests of sweet potatoes in the world. with free trade between the united states and the u.s.-controlled mariana islands, c. formicarius has spread along with this commodity. because of the cryptic nature of the larvae and nocturnal activity of the adults, and the cancellation of long-residual pesticides, this pest has become increasingly difficult to control. therefore, the present study sought t ... | 2014 | 25111763 |
| a molecular tool for detection and tracking of a potential indigenous beauveria bassiana strain for managing emerald ash borer populations in canada. | emerald ash borer is an invasive species from asia. beauveria bassiana strain l49-1aa is being tested for the control of emerald ash borer in canada, using an autocontamination trapping system. we have developed a simplified allele discrimination polymerase chain reaction (pcr) assay to screen b. bassiana strain, l49-1aa from other beauveria species by targeting the inter-strain genetic differences in 5' end of ef1-α gene of the genus beauveria. a single nucleotide polymorphism (snp) site, t→c w ... | 2014 | 25110340 |
| the entomopathogenic fungal endophytes purpureocillium lilacinum (formerly paecilomyces lilacinus) and beauveria bassiana negatively affect cotton aphid reproduction under both greenhouse and field conditions. | the effects of two entomopathogenic fungal endophytes, beauveria bassiana and purpureocillium lilacinum (formerly paecilomyces lilacinus), were assessed on the reproduction of cotton aphid, aphis gossypii glover (homoptera:aphididae), through in planta feeding trials. in replicate greenhouse and field trials, cotton plants (gossypium hirsutum) were inoculated as seed treatments with two concentrations of b. bassiana or p. lilacinum conidia. positive colonization of cotton by the endophytes was c ... | 2014 | 25093505 |
| occurrence of entomopathogenic fungi in tejocote (crataegus mexicana) orchard soils and their pathogenicity against rhagoletis pomonella. | to determine the abundance and diversity of entomopathogenic fungi in tejocote orchard soils and evaluate their ability to infect rhagoletis pomonella walsh., the main pest of tejocote. | 2014 | 25081747 |
| microbial metabolism of danazol: a contribution to doping analysis. | microbial metabolism of danazol (17alpha-pregna-2,4-dien-20-yno[2,3-d]isoxazol-17beta-ol) by beauveria bassiana atcc 7159 and glyocladium viride atcc 10097 afforded four metabolites. the isolated metabolites were identified by different spectroscopic techniques as 6beta-hydroxy danazol, which is a not yet reported danazol metabolite, 17beta-hydroxy-17alpha-pregn-4-en-20-yn-3-one (ethisterone) and 17beta-hydroxy-2alpha-(hydroxymethyl)-17alpha-pregn-4-en-20-yn-3-one (2alpha-hydroxymethyl ethistero ... | 2014 | 25069163 |
| prevalence of beauveria pseudobassiana among entomopathogenic fungi isolated from the hard tick, ixodes ricinus. | human and animal disease-transmitting hard ticks (acari: ixodidae) are of great concern for public health and animal farming. alternatives to tick control by chemical acaricides are urgently needed, and one intensively evaluated biocontrol strategy is based on the use of tick-pathogenic filamentous fungi. an indispensable prerequisite for the development of tick-derived fungal isolates into registered myco-acaricides is their sound taxonomic characterisation. a set of fungal strains isolated fro ... | 2014 | 25065606 |
| enhanced conidial thermotolerance and field efficacy of beauveria bassiana by saccharides. | insecticide efficacy of beauveria bassiana conidia was improved by optimizing the concentrations of conidial heat-protective saccharides (glucose, sucrose, maltose, trehalose, α-lactose, and mannitol) using response surface methodology. two field trials in tea gardens were carried out to control leafhopper (empoasca vitis) by spraying b. bassiana conidia together with the optimized saccharides (0.26 g glucose, 0.28 g lactose, 0.24 g mannitol per ml). in the field studies, b. bassiana conidia wer ... | 2014 | 25062642 |
| antifungal activity of stenotrophomonas maltophilia in stomoxys calcitrans larvae. | the microbiota present in stomoxys calcitrans larvae may assist their survival in contaminated environments through production of inhibitory substances. bacteriological identification methods, the polymerase chain reaction (pcr) and scanning electron microscopy (sem) were used to detect a bacterium naturally present in mucus and macerated s. calcitrans larvae. the antifungal activity was determined based on the results from disk diffusion tests on an artificial solid medium. the bacterium was id ... | 2014 | 25054498 |
| comparison of in vitro and in vivo phototoxicity tests with s-(-)-10,11-dihydroxyfarnesic acid methyl ester produced by beauveria bassiana kacc46831. | beauveria bassiana is a fungi that is well-known for demonstrating a resistance to environmental change. to confirm whether s-(-)-10,11-dihydroxyfarnesic acid methyl ester (dhfame) produced by beauveria bassiana kacc46831 causes phototoxicity when used for cosmetic purposes due to its anti-tyrosinase activity, we conducted in vitro and in vivo phototoxicity tests. there were no significant changes or damage observed in the compound-treated group with regards to skin phototoxicity, while 8-methox ... | 2014 | 25054007 |
| s-(-)-10,11-dihydroxyfarnesoic acid methyl ester inhibits melanin synthesis in murine melanocyte cells. | the development of antimelanogenic agents is important for the prevention of serious aesthetic problems such as melasmas, freckles, age spots, and chloasmas. in the course of screening for melanin synthesis inhibitors, we found that the culture broth from an insect morphopathogenic fungus, beauveria bassiana cs1029, exhibits potent antimelanogenic activity. we isolated and purified an active metabolite and identified it as s-(-)-10,11-dihydroxyfarnesoic acid methyl ester (dhfame), an insect juve ... | 2014 | 25046747 |
| interactions between the entomopathogenic fungi beauveria bassiana (ascomycota: hypocreales) and the aphid parasitoid diaeretiella rapae (hymenoptera: braconidae) on myzus persicae (hemiptera: aphididae). | the interactions between the entomopathogenic fungus beauveria bassiana (balsamo-crivelli) vuillemin (ascomycota: hypocreales) and the aphid parasitoid diaeretiella rapae mcintoch (hymenoptera: braconidae) were evaluated under laboratory conditions. nymphs of myzus persicae sulzer (hemiptera: aphididae) were first exposed to parasitoid females for 24 h and then 0, 24, and 48 h afterwards sprayed with a solution of b. bassiana. likewise, aphids were also sprayed with b. bassiana and then exposed ... | 2014 | 25026650 |
| bbsnf1 contributes to cell differentiation, extracellular acidification, and virulence in beauveria bassiana, a filamentous entomopathogenic fungus. | snf1/ampk protein kinase plays important roles in fungal development and activation of catabolite-repressed genes. in this study, we characterized the role of the beauveria bassiana snf1 ortholog. the vegetative growth of the δbbsnf1 mutant was reduced by 16 to 50 % on diverse carbon/nitrogen sources. transcriptional analysis revealed a collection of proteases and chitinases that were not induced when the mutant was grown on complex carbon/nitrogen sources. bbsnf1 also contributes to extracellul ... | 2014 | 25016341 |
| pathogenicity bioassays of isolates of beauveria bassiana on rhynchophorus ferrugineus. | the control of rhynchophorus ferrugineus (olivier), the main palm pest in the mediterranean basin, is problematic because of its biology and the current restrictions in many european countries on the use of chemical insecticides in urban areas. entomopathogenic fungi have been studied as potential biological control agents, but information on their natural incidence is limited. strains of beauveria bassiana (balsamo) vuillemin were isolated from symptomatic insects collected on dead palms, and t ... | 2015 | 24990249 |
| three α-1,2-mannosyltransferases contribute differentially to conidiation, cell wall integrity, multistress tolerance and virulence of beauveria bassiana. | members of α-1,2-mannosyltransferase (ktr) family are required for protein o-mannosylation for the elongation of ser/thr mannose residues in yeasts but functionally unknown in most filamentous fungi. here we characterized the functions of the ktr orthologues ktr1, ktr4 and kre2/mnt1 in beauveria bassiana, a filamentous enotmopathogen, and found that they were positive, but differential, mediators of many biological traits. inactivation of ktr4 and kre2 resulted in 92% reduction of conidial yield ... | 2014 | 24981201 |
| bbmsn2 acts as a ph-dependent negative regulator of secondary metabolite production in the entomopathogenic fungus beauveria bassiana. | fungal secondary metabolites are chemical compounds important for development, environmental adaptation and for potential biotechnological and pharmaceutical applications. oosporein, a red-pigmented benzoquinone, produced by many fungal insect pathogenic beauveria spp., shows remarkable functional diversity, displaying antimicrobial, antiviral and even anti-proliferative activities. a homologue of the msn2/seb1 transcription factor was identified in a beauveria bassiana random t-dna insertion li ... | 2015 | 24965521 |
| immune priming and pathogen resistance in ant queens. | growing empirical evidence indicates that invertebrates become more resistant to a pathogen following initial exposure to a nonlethal dose; yet the generality, mechanisms, and adaptive value of such immune priming are still under debate. because life-history theory predicts that immune priming and large investment in immunity should be more frequent in long-lived species, we here tested for immune priming and pathogen resistance in ant queens, which have extraordinarily long life span. we expose ... | 2014 | 24963375 |
| screening of liquid media and fermentation of an endophytic beauveria bassiana strain in a bioreactor. | a novel approach for biological control of insect pests could be the use of the endophytic entomopathogenic beauveria bassiana isolate atp-02. for the utilization of the endophyte as a commercial biocontrol agent, the fungus has to be mass-produced. b. bassiana was raised in shake flask cultures to produce high concentrations of total spores (ts), which include blastospores (bs) and submerged conidiospores (scs). the highest concentration of 1.33×10(9) ts/ml and the highest yield of 5.32×10(10) ... | 2014 | 24949278 |
| evaluation of the effectiveness of entomopathogens for the management of wireworms (coleoptera: elateridae) on spring wheat. | wireworms, the larval stage of click beetles (coleoptera: elateridae), are serious soil dwelling pests of small grains, corn, sugar beets, and potatoes. limonius californicus and hypnoidus bicolor are the predominant wireworm species infesting wheat in montana, particularly in the 'golden triangle' area of north-central montana. wireworm populations in field crops are increasing, but currently available insecticides provide only partial control, and no alternative management tools exist. in our ... | 2014 | 24944009 |
| three types of geranylgeranyl diphosphate synthases from the medicinal caterpillar fungus, cordyceps militaris (ascomycetes). | geranylgeranyl diphosphate synthase (ggpps) is a key enzyme in the carotenoid biosynthetic pathway, catalyzing the synthesis of its c20 precursor. in the present study, three types of ggpps genes were cloned and analyzed from the caterpillar medicinal fungus cordyceps militaris, a valued carotenoid-producing species. the sequences were named as ggpps727, ggpps191, and ggpps595. the open reading frame codes for predicted polypeptides of 464, 550, and 431 aa. three predicted ggppss had a high simi ... | 2014 | 24941033 |
| effect of fungal colonization of wheat grains with fusarium spp. on food choice, weight gain and mortality of meal beetle larvae (tenebrio molitor). | species of fusarium have significant agro-economical and human health-related impact by infecting diverse crop plants and synthesizing diverse mycotoxins. here, we investigated interactions of grain-feeding tenebrio molitor larvae with four grain-colonizing fusarium species on wheat kernels. since numerous metabolites produced by fusarium spp. are toxic to insects, we tested the hypothesis that the insect senses and avoids fusarium-colonized grains. we found that only kernels colonized with f. a ... | 2014 | 24932485 |
| wee1 and cdc25 control morphogenesis, virulence and multistress tolerance of beauveria bassiana by balancing cell cycle-required cyclin-dependent kinase 1 activity. | modification of cell cycle in entomopathogenic fungi is likely crucial for host infection and environmental adaptation. here we show that wee1 and cdc25 can balance cell cycle-required cyclin-dependent kinase 1 (cdk1) activity in beauveria bassiana. the cdk1 phosporylation signal was strong in δcdc25 but very weak in δwee1 and absent in δwee1δcdc25. consequently, cell cycles, septation patterns and many septation-dependent gene transcripts of these mutants were reversely changed. hyphal cells we ... | 2015 | 24910927 |
| myco-synthesis of silver nanoparticles using beauveria bassiana against dengue vector, aedes aegypti (diptera: culicidae). | the efficacy of silver synthesized biolarvicide with the help of entomopathogenic fungus, beauveria bassiana, was assessed against the different larval instars of dengue vector, aedes aegypti. the silver nanoparticles were observed and characterized by a scanning electron microscope (sem) and energy-dispersive x-ray (edx). a surface plasmon resonance band was observed at 420 nm in uv-vis spectrophotometer. the characterization was confirmed by shape (spherical), size 36.88-60.93 nm, and edx spec ... | 2014 | 24861012 |
| differentially expressed genes in the cuticle and hemolymph of the silkworm, bombyx mori, injected with the fungus beauveria bassiana. | the most important pathogenic fungus of the silkworm, bombyx mori l. (lepidoptera: bombycidae), is beauveria bassiana (balsamo-crivelli ) vuillemin (hypocreales: clavicipitaceae), which causes significant damage to sericulture production. therefore, diagnosing fungal disease and developing new control measures are crucial to silk production. to better understand the responsive and interactive mechanisms between the host silkworm and this fungus, variations in silkworm gene expression were invest ... | 2013 | 24794288 |
| mycosis inhibits cannibalism by melanoplus sanguinipes, m. differentialis, schistocerca americana, and anabrus simplex. | cannibalism is common among the acrididae and the mormon cricket, anabrus simplex haldeman (orthoptera: tettigoniidae). this behavior has been proposed as a mechanism for the horizontal transmission of microsporida and entomopathogenic fungi. aanecdotal observations suggested that the migratory grasshopper, melanoplus sanguinipes fabricius (acrididae), and a. simplex did not eat cadavers that had been killed by insect pathogenic fungi. the hypothesis tested was that a. simplex or m. sanguinipes ... | 2013 | 24786183 |
| [identification of spore germination and virulence related biomarkers from beauveria bassiana using an lc-ms-based metabolomic technique]. | to identify biomarkers associated with germination and virulence of beauveria bassiana. | 2014 | 24783852 |
| sustainable management tactics for control of phyllotreta cruciferae (coleoptera: chrysomelidae) on canola in montana. | the crucifer flea beetle, phyllotreta cruciferae (goeze) (coleoptera: chrysomelidae), has recently emerged as a serious pest of canola (brassica napus l.) in montana. the adult beetles feed on canola leaves, causing many small holes that stunt growth and reduce yield. in 2013, damage to canola seedlings was high (approximately 80%) in many parts of montana, evidence that when flea beetles emerge in large numbers, they can quickly destroy a young canola crop. in the current study, the effectivene ... | 2014 | 24772547 |
| a novel approach: beauveria bassiana granules applied to nursery soil for management of rice water weevils in paddy fields. | spraying of entomopathogenic fungi formulated as wettable powders and suspension concentrates based on oils or water has been used for the management of crop pests. however, the use of fungal granules to control paddy field pests has not been fully explored. herein, several beauveria bassiana isolates (erl1170, erl1578 and erl836) were produced as granules using a millet-based solid culture. the granules were applied to the rice nursery 3 days before transplanting, and their control efficacy aga ... | 2014 | 24771507 |
| specific diversity of the entomopathogenic fungi beauveria and metarhizium in mexican agricultural soils. | prior knowledge of the local population structure of entomopathogenic fungi is considered an important requisite when developing microbial control strategies against major pests of crops such as white grubs. an extensive survey in the estate of guanajuato, one of the main agricultural regions of mexico, was carried out to determine the abundance and diversity of entomopathogenic fungi in soil. soil collected from 11 locations was baited for entomopathogenic fungi using galleria mellonella. in ad ... | 2014 | 24769124 |
| adenylate cyclase orthologues in two filamentous entomopathogens contribute differentially to growth, conidiation, pathogenicity, and multistress responses. | adenylate cyclase (ac) is a core element of camp signalling network. here we show functional diversity and differentiation of beauveria bassiana ac (bbac) and metarhizium robertsii ac (mrac). severe growth defects occurred in δbbac and δmrac grown on nutrition-rich sday and several minimal media but were largely alleviated by adding camp to sday. conidial yield increased greatly in δbbac but decreased in δmrac. during colony growth, δbbac was highly sensitive to oxidation, high osmolarity, cell ... | 2014 | 24742837 |
| the connection of protein o-mannosyltransferase family to the biocontrol potential of beauveria bassiana, a fungal entomopathogen. | o-mannosylation dependent on the protein o-mannosyltransferase (pmt) family is an essential post-translational modification process in eukaryotes, but their connection to the biocontrol potential of a filamentous entomopathogen against arthropod pests is not understood. here, we characterized the functions of three pmt orthologues (pmt1, pmt2 and pmt4) in the pmt family of beauveria bassiana and found that they were positive, but differential, regulators of the fungal growth, conidiation, multi- ... | 2014 | 24727441 |
| phytochrome controls conidiation in response to red/far-red light and daylight length and regulates multistress tolerance in beauveria bassiana. | phytochromes (phy) in filamentous fungi are group viii histidine kinases that share a unique n-terminal photosensory core, but their functions are largely unknown. here we show that beauveria bassiana phy (bbphy) is functionally vital for growth, conidiation and multistress tolerance of the fungal entomopathogen lacking sexual stage. colony growth of δbbphy was significantly slower in a nutrition-rich medium but faster in several minimal media. conidial yield of δbbphy in the rich medium increas ... | 2014 | 24725588 |
| host-to-pathogen gene transfer facilitated infection of insects by a pathogenic fungus. | metarhizium robertsii is a plant root colonizing fungus that is also an insect pathogen. its entomopathogenicity is a characteristic that was acquired during evolution from a plant endophyte ancestor. this transition provides a novel perspective on how new functional mechanisms important for host switching and virulence have evolved. from a random t-dna insertion library, we obtained a pathogenicity defective mutant that resulted from the disruption of a sterol carrier gene (mr-npc2a). phylogene ... | 2014 | 24722668 |
| vasorelaxant activity of 7-β-o-glycosides biosynthesized from flavonoids. | in this work we report the vasorelaxant activity of 7-β-o-glycosides obtained with biosynthesis of naringenin-7-β-o-glycoside (3) and quercetin-7-β-o-glycoside (4). these compounds were obtained from naringenin (1) and quercetin (2) glycosylation catalyzed by beauveria bassiana atcc 7159. screening of the best strain as a catalyst for glycosylation was carried out and the reaction conditions established. cultures were grown in pdsm medium for 7 days at 27 °c. after purification by reverse-phase ... | 2014 | 24704375 |
| fitness consequences of larval exposure to beauveria bassiana on adults of the malaria vector anopheles stephensi. | entomopathogenic fungi have shown to be effective in biological control of both larval and adult stages of malaria mosquitoes. however, a small fraction of mosquitoes is still able to emerge after treatment with fungus during the larval stage. it remains unclear whether fitness of these adults is affected by the treatment during the larval stage and whether they are still susceptible for another treatment during the adult stage. therefore, we tested the effects of larval exposure to the entomopa ... | 2014 | 24694552 |
| establishment of fungal entomopathogens beauveria bassiana and bionectria ochroleuca (ascomycota: hypocreales) as endophytes on artichoke cynara scolymus. | entomopathogenic fungi (epf) are commonly found in diverse habitats and are known to cause mycoses in many different taxa of arthropods. various unexpected roles have been recently reported for fungal entomopathogens, including their presence as fungal endophytes, plant disease antagonists, rhizosphere colonizers and plant growth promoting fungi. in tunisia, a wide range of indigenous epf isolates from different species, such as beauveria bassiana and bionectria ochroleuca, were found to occur i ... | 2014 | 24681358 |
| physiological aspects of the interaction of multi-walled carbon nanotubes with beauveria bassiana (balsamo) vuillem in entomopathogenic spores. | the objective of the study was to evaluate the in vitro effect of aqueous suspensions of commercial multi-walled carbon nanotubes (mwcnts) on spores of three entomopathogenic fungus species of beauveria bassiana. spores were placed in contact with mwcnts for 1, 24, 72 and 216 h and then an in vitro evaluation in the subculture was performed on the linear growth of the vegetative mycelium of the spores after the contact with nanomaterial, as well as its sporulation after completion of the culture ... | 2014 | 24679095 |
| bbssk1, a response regulator required for conidiation, multi-stress tolerance, and virulence of beauveria bassiana. | ssk1-type response regulator proteins are the core elements of histidine-to-aspartate systems that mediate fungal stress tolerance, a determinant to the biocontrol potential of fungal entomopathogens. we characterized the functions of beauveria bassiana ssk1 (bbssk1) by analyzing multi-phenotypic changes in δbbssk1 and differentially expressed genes in the digital gene expression (dge) libraries of δbbssk1 and wild-type constructed under osmotic stress. the bbssk1 disruption caused 25 % reductio ... | 2014 | 24633371 |
| transcriptome analysis of silkworm, bombyx mori, during early response to beauveria bassiana challenges. | host-pathogen interactions are complex processes and it is a central challenge to reveal these interactions. fungal infection of silkworm, bombyx mori, may induce a variety of responsive reaction. however, little is known about the molecular mechanism of silkworm immune response against the fungal infection. to obtain an overview of the interaction between silkworm and an entomopathogenic fungus beauveria bassiana, digital gene expression profiling, a tag based high-throughput transcriptome sequ ... | 2014 | 24618587 |
| the transcriptional co-activator multiprotein bridging factor 1 from the fungal insect pathogen, beauveria bassiana, mediates regulation of hyphal morphogenesis, stress tolerance and virulence. | multiprotein bridging factors (mbfs) are evolutionarily highly conserved cofactors that link tata-binding protein and the associated basal transcription machinery to transcription factors. the filamentous fungus, beauveria bassiana, has a multipotential lifestyle capable of growing as a saprophyte, plant endophyte and insect pathogen. deletion of the single b. bassiana mbf homologue (bbmbf1) affected fungal growth and hyphal morphogenesis, stress response and virulence. compared with wild type, ... | 2014 | 24612420 |
| evaluation of etest performed in mueller-hinton agar supplemented with glucose for antifungal susceptibility testing of clinical isolates of filamentous fungi. | although reference broth microdilution protocol is currently available for filamentous fungi antifungal susceptibility testing (afst), simpler alternatives as etest(®) tend to be favoured in clinical routine, making their validation of utmost importance. in this study, etest(®) method using 2% glucose supplemented muller-hinton agar was compared to the clinical and laboratory standards institute (clsi) m38-a2 protocol for filamentous fungi afst. the echinocandins, caspofungin and anidulafungin, ... | 2014 | 24570038 |
| histopathological and molecular insights into the ovicidal activities of two entomopathogenic fungi against two-spotted spider mite. | entomopathogenic fungi can infect and kill spider mite eggs but their ovicidal activities are poorly understood. here we gain histopathogenical and molecular insights into the ovicidal activities of beauveria bassiana and isaria fumosorosea against the two-spotted spider mite, tetranychus urticae. scanning electronic microscopy indicated successful adhesion and germination of fungal conidia on egg shell at 24h post-spray (hps). germ tubes of both fungi could penetrate into egg shell with penetra ... | 2014 | 24566192 |
| effect of beauveria bassiana infection on detoxification enzyme transcription in pyrethroid resistant anopheles arabiensis: a preliminary study. | fungal biopesticides are of great interest to vector control scientists as they provide a novel and environmentally friendly alternative to insecticide use. the aim of this study was to determine whether genes associated with pyrethroid resistance in anopheles arabiensis from sudan and south africa are further induced following exposure to the entomopathogenic fungus, beauveria bassiana (strain gha). | 2014 | 24561325 |
| characterization and biocontrol potential of entomopathogenic fungus, beauveria bassiana isolates against spilarctia obliqua. | beauveria bassiana is a known natural enemy of a number of insect pests of crop plants. in order to screen different isolates of any given entomopathogens molecular markers provide a means for constructing the molecular phylogeny, diversity and link to virulent phenotypes. eight isolates of b. bossiona isolated from different insect hosts and from different location at pantnagar (uttrakhand) were characterized by pcr-based rapd markers. bioassays were conducted by using first, second and third i ... | 2013 | 24558805 |
| the hidden habit of the entomopathogenic fungus beauveria bassiana: first demonstration of vertical plant transmission. | beauveria bassiana strain 04/01-tip, obtained from a larva of the opium poppy stem gall wasp iraella luteipes (hymenoptera; cynipidae), endophytically colonizes opium poppy (papaver somniferum l.) plants and protects them against this pest. the goal of this study was to monitor the dynamics of endophytic colonization of opium poppy by b. bassiana after the fungus was applied to the seed and to ascertain whether the fungus is transmitted vertically via seeds. using a species-specific nested pcr p ... | 2014 | 24551242 |
| the gpi-anchored protein ecm33 is vital for conidiation, cell wall integrity, and multi-stress tolerance of two filamentous entomopathogens but not for virulence. | ecm33 is one of several glycosylphosphatidylinositol (gpi)-anchored proteins. this protein is known to be involved in fungal cell wall integrity, but its contribution to multi-stress tolerance is largely unknown. here we characterized the functions of two ecm33 orthologues, i.e., bbecm33 in beauveria bassiana and mrecm33 in metarhizium robertsii. bbecm33 and mrecm33 were both confirmed as gpi-anchored cell wall proteins in immunogold localization. single-gene disruptions of bbecm33 and mrecm33 c ... | 2014 | 24549768 |
| efficacy of two fungus-based biopesticide against the honeybee ectoparasitic mite, varroa destructor. | the varroa mite, varroa destructor (anderson and trueman) (acari: varroidae), is known as the most serious ectoparasitic mite on honeybee, apis mellifera (hymenoptera: apidae) in the world. based on the spores of entomopathogenic fungi, two commercial preparations; bioranza (metarhizium anisopliae) and biovar (beauveria bassiana) were evaluated through application into the hives against varroa mite. data showed significant differences between treatments with bioranza and biovar, the results were ... | 2013 | 24498835 |
| laboratory assessment of beauveria bassiana (hypocreales: clavicipitaceae) strain gha for control of listronotus maculicollis (coleoptera: curculionidae) adults. | bioassays were designed to evaluate beauveria bassiana (balsamo) vuillemin strain gha against listronotus maculicollis (kirby) adults. b. bassiana and its "inert" carrier oil in the product botanigard and the inert carrier oil alone provided 99 and 96% mortality, respectively, in petri dish assays 1 d after treatment when applied in 1 ml water. when the same treatments were applied in 0.5 ml of carrier water, mortality was only 1.4 and 0.7%, respectively, 1 d after treatment. after 10 d in petri ... | 2013 | 24498729 |
| three mitogen-activated protein kinases required for cell wall integrity contribute greatly to biocontrol potential of a fungal entomopathogen. | bck1, mkk1 and slt2 are three mitogen-activated protein (map) kinases constituting cell wall integrity (cwi) pathway that may control multi-stress responses via crosstalk with high-osmolarity glycerol (hog) pathway in budding yeast. in this study, bck1, mkk1 and slt2 orthologues in beauveria bassiana were confirmed as the three-module cascade essential for cwi because cell wall impairment occurred in the hyphae and conidia of δbck1, δmkk1 and δslt2 examined in multiple experiments. strikingly, a ... | 2014 | 24498410 |
| production of conidia of beauveria bassiana in solid-state culture: current status and future perspectives. | beauveria bassiana is an important entomopathogenic fungus widely commercialized in the world. recent progress and achievements on conidia production have focused on a yield goal of 10(9) to 10(10) conidia per gram of dry substrate. due to cost-competitive perspectives, these yields should be associated with better production rates or productivities. this study presents a review of relevant studies of b. bassiana conidia production on solid-state cultures and the parameters that should be taken ... | 2015 | 24494702 |
| biological control of aphid using fungal culture and culture filtrates of beauveria bassiana. | aphids are one of the most destructive pests in crop production such as pepper, cucumber, and eggplants. the importance of entomopathogenic fungi as alternative pest control agents is increasing. conidia of entomopathogenic fungi are influenced by environmental conditions, such as temperature and relative humidity, and cause slow and fluctuating mortality. these factors have prevented wider application and use of biocontrol agents. for investigation of means of mitigation of such problems, we co ... | 2013 | 24493943 |
| microbial baeyer-villiger oxidation of 5α-steroids using beauveria bassiana. a stereochemical requirement for the 11α-hydroxylation and the lactonization pathway. | beauveria bassiana kch 1065, as was recently demonstrated, is unusual amongst fungal biocatalysts in that it converts c19 3-oxo-4-ene and 3β-hydroxy-5-ene as well as 3β-hydroxy-5α-saturated steroids to 11α-hydroxy ring-d lactones. the baeyer-villiger monooxygenase (bvmo) of this strain is distinguished from other enzymes catalyzing bvo of steroidal ketones by the fact that it oxidizes solely substrates with 11α-hydroxyl group. the current study using a series of 5α-saturated steroids (androstero ... | 2014 | 24486796 |
| pathogenicity, ovicidal action, and median lethal concentrations (lc 50 ) of entomopathogenic fungi against exotic spiralling whitefly, aleurodicus dispersus russell. | biological control using entomopathogenic fungi could be a promising alternative to chemical control. entomopathogenic fungi, beauveria bassiana (balsamo) vuillemin, metarhizium anisopliae (metschnikoff) sorokin, lecanicillium lecanii (zimmerm.) zare and gams, and paecilomyces fumosoroseus (wize) brown and smith, were tested for their pathogenicity, ovicidal effect, and median lethal concentrations (lc50) against exotic spiralling whitefly, aleurodicus dispersus russell. the applications were ma ... | 2013 | 24455279 |
| an entomopathogenic strain of beauveria bassiana against frankliniella occidentalis with no detrimental effect on the predatory mite neoseiulus barkeri: evidence from laboratory bioassay and scanning electron microscopic observation. | among 28 isolates of beauveria bassiana tested for virulence against f. occidentalis in laboratory bioassays, we found strain sz-26 as the most potent, causing 96% mortality in adults at 1×10(7) ml(-1)conidia after 4 days. the effect of the strain sz-26 on survival, longevity and fecundity of the predatory mite neoseiulus (amblyseius) barkeri hughes were studied under laboratory conditions. the bioassay results showed that the corrected mortalities were less than 4 and 8% at 10 days following in ... | 2014 | 24454744 |
| immune response of chilo suppressalis walker (lepidoptera: crambidae) larvae to different entomopathogenic fungi. | the current study reports mortality and effects on cellular immune response of several entomopathogenic fungi including isoleates bb1, bb2 and bb3 of beauveria bassiana, metarhizium anisopliae, isaria fumosoroseus and lecanicilium lecanii against larvae of chilo suppressalis. prohemocytes, granulocytes, plasmatocytes and oenocytoids were identified as the main circulating hemocytes in the hemolymph of larvae using giemsa staining solution. entomopathogenic fungi caused differential mortality on ... | 2014 | 24447729 |
| nutritional optimization of a native beauveria bassiana isolate (hq917687) pathogenic to housefly, musca domestica l. | a new beauveria bassiana isolate, showing high activity against musca domestica l. (diptera: muscidae) adults (mortality-100.0 %), larvae (mortality-72.3 %) and pupae (infection in emerged flies-96.7 %) was used. the isolate was subjected to a combinational approach towards selection of process parameters for its growth optimization. initial screening of several carbon and nitrogen sources revealed glucose and nano3 as the most suitable source for optimal biomass and spore production. further, o ... | 2013 | 24431571 |
| evaluation of the entomopathogenic fungi beauveria bassiana, metarhizium anisopliae, and isaria fumosorosea for control of sitophilus oryzae. | the entomopathogenic fungi beauveria bassiana, metarhizium anisopliae, and isaria fumosorosea were tested against the stored-grain pest sitophilus oryzae. the fungi were isolated from the soil (from three locations in attica, greece: b. bassiana from tatoion, m. anisopliae from marathon, and i. fumosorosea from aghios stefanos) using larvae of galleria mellonella as bait. suspensions of 2.11 × 10(7) and 2.11 × 10(8), 1.77 × 10(7) and 1.77 × 10(8), and 1.81 × 10(7) and 1.81 × 10(8) conidia per ml ... | 2014 | 24406003 |
| expression and promoter characterization of bbpacc, a ph response transcription factor gene of the entomopathogenic fungus beauveria bassiana. | to survive, the entomopathogenic fungus beauveria bassiana, which shows promise as a biocontrol agent for a variety of pests, including agricultural and forestry pests and vectors of human pathogens, must tailor gene expression to the particular ph of its environment. the ph response transcription factor gene bbpacc and its flanking sequence were cloned from this fungus. quantitative reverse transcription (rt)-pcr revealed that it is highly induced by alkaline ph and salt stress, and the express ... | 2014 | 24336463 |
| ubiquity of insect-derived nitrogen transfer to plants by endophytic insect-pathogenic fungi: an additional branch of the soil nitrogen cycle. | the study of symbiotic nitrogen transfer in soil has largely focused on nitrogen-fixing bacteria. vascular plants can lose a substantial amount of their nitrogen through insect herbivory. previously, we showed that plants were able to reacquire nitrogen from insects through a partnership with the endophytic, insect-pathogenic fungus metarhizium robertsii. that is, the endophytic capability and insect pathogenicity of m. robertsii are coupled so that the fungus acts as a conduit to provide insect ... | 2014 | 24334669 |
| molecular characterization of a novel victorivirus from the entomopathogenic fungus beauveria bassiana. | new zealand isolates of the entomopathogenic fungus beauveria were examined for the presence of dsrnas and virus-like particles. seven out of nine isolates contained one or more high-molecular-weight dsrnas and all seven contained isometric virus particles ranging in size from 30 to 50 nm. b. bassiana isolate icmp#6887 contained a single dsrna band of ~6 kb and isometric virus-like particles of ~50 nm in diameter. sequencing revealed that the virus from icmp#6887 had a genome of 5,327 nt with tw ... | 2014 | 24327093 |
| ablation of the crea regulator results in amino acid toxicity, temperature sensitivity, pleiotropic effects on cellular development and loss of virulence in the filamentous fungus beauveria bassiana. | for most organisms, carbon and nitrogen uptake are essential for growth, development and, where applicable, pathogenesis. the role of the carbon catabolite repressor transcription factor homologue bbcrea in the entomopathogenic fungus beauveria bassiana was investigated. deletion of bbcrea resulted in pleiotropic effects, including nutrient toxicity, leading to a novel cell lytic phenotype. fungal growth in rich media and minimal media containing select amino acids/peptides was severely compromi ... | 2014 | 24320078 |
| genetic variability of beauveria bassiana and a dna marker for environmental monitoring of a highly virulent isolate against cosmopolites sordidus. | the banana weevil cosmopolites sordidus (germar) is one of a number of pests that attack banana crops. the use of the entomopathogenic fungus beauveria bassiana as a biological control agent for this pest may contribute towards reducing the application of chemical insecticides on banana crops. in this study, the genetic variability of a collection of brazilian isolates of b. bassiana was evaluated. samples were obtained from various geographic regions of brazil, and from different hosts of the c ... | 2012 | 24293712 |
| investigations on a hyper-proteolytic mutant of beauveria bassiana: broad substrate specificity and high biotechnological potential of a serine protease. | a new strain of beauveria bassiana was identified on the basis of the 18s rrna gene sequence homology. this strain, called p2, is a spontaneously arisen mutant that was isolated after successive sub-culturing the wild-type b. bassiana p1 strain. p2 showed hyper-production of extracellular protease(s) as much as ninefold more than p1. an extracellular protease (sbp) having a molecular weight of 32 kda was purified from the p2 strain. sbp was completely inhibited by the phenyl methyl sulphonyl flu ... | 2014 | 24267723 |
| synergistic interaction between the fungus beauveria bassiana and desiccant dusts applied against poultry red mites (dermanyssus gallinae). | the poultry red mite, dermanyssus gallinae, is a major pest in egg production, feeding on laying hens. widely used non-chemical control methods include desiccant dusts, although their persistence under field conditions is often short. entomopathogenic fungi may also hold potential for mite control, but these fungi often take several days to kill mites. laboratory experiments were carried out to study the efficacy of 3 types of desiccant dusts, the fungus beauveria bassiana and combinations of th ... | 2014 | 24253584 |
| the potential for controlling pangaeus bilineatus (heteroptera: cydnidae) using a combination of entomopathogens and an insecticide. | the peanut burrower bug, pangaeus bilineatus (say), is an important pest of peanut (arachis hypogaea l.) in the southern united states. current control methods for this pest, which are based on the use of chemical insecticides, have not been successful. our objective was to determine if entomopathogens applied alone or in combination with a standard chemical insecticide would provide superior levels of p. bilineatus mortality compared with the standard chemical applied alone. specifically, we in ... | 2013 | 24224248 |
| evaluation of beauveria bassiana for management of citrus thrips (thysanoptera: thripidae) in california blueberries. | citrus thrips, scirtothrips citri (moulton), is a plant-feeding pest most widely recognized for causing damage to citrus and mango fruits. this insect has broadened its host range to become a significant pest of commercial blueberries grown in the san joaquin valley of california. we evaluated beauveria bassiana (balsamo) for control of citrus thrips in blueberries grown under two watering regimes (drip irrigation with and without overhead sprinklers) and using two fungal formulations (commercia ... | 2013 | 24224239 |
| beauveria bassiana strains for biological control of cosmopolites sordidus (germ.) (coleoptera: curculionidae) in plantain. | the objective of this study was to select strains of beauveria bassiana for controlling cosmopolites sordidus (germ.) in plantain farms (cv. terra) of the "recôncavo" and southern regions in the state of bahia, brazil. the virulence of 32 b. bassiana isolates against c. sordidus was determined under laboratory conditions. three isolates (cnpmf 407, cnpmf 218, and cnpmf 416) were selected for evaluation under field conditions in plantations located in the counties of mutuípe and wenceslau guimarã ... | 2013 | 24187659 |
| cytokinesis-required cdc14 is a signaling hub of asexual development and multi-stress tolerance in beauveria bassiana. | a dual-specificity, paralogue-free cdc14 phosphatase was located in the nuclei of beauveria bassiana (filamentous entomopathogen) and functionally characterized. inactivation of cdc14 caused defective cytokinesis due to multinucleate cells formed in δcdc14 and 89% decrease of blastospore production, followed by slower growth and a loss of ≥ 96% conidial yield under normal conditions. these defects coincided well with drastic down-regulation of 25 genes required for mitosis and conidiation. moreo ... | 2013 | 24169500 |
| oxidative stress response of beauveria bassiana to bordeaux mixture and its influence on fungus growth and development. | chemical fungicides used to manage plant diseases may negatively affect beneficial fungi such as entomopathogens. in this study, the participation of the antioxidative system in the entomopathogenic fungus beauveria bassiana exposed to the copper-based bordeaux mixture fungicide and its relation with fungus growth and development were examined. the fungus was grown in submerged culture containing bordeaux mixture at the recommended dose. within the first 24 h of elicitation, the vegetative growt ... | 2014 | 24166767 |
| chytrid mycoparasitism of entomophthoralean azygospores. | mycoparasitism - when one fungus parasitizes another - has been reported to affect beauveria bassiana and mycorrhizal fungi in the field. however, mycoparasitism of any fungi in the order entomophthorales has never been reported before now. the majority of entomophthoralean species persist as resting spores (either zygospores or azygospores) in the environment and dormant entomophthoralean resting spores (whether formed as zygospores or azygospores) are thought to be especially well adapted for ... | 2013 | 24140499 |
| evaluation of eye irritation by s-(-)-10,11-dihydroxyfarnesic acid methyl ester secreted by beauveria bassiana cs1029. | the aim of this study was to investigate whether s-(-)-10,11-dihydroxyfarnesic acid methyl ester produced by cell subtype beauveria bassiana cs1029 causes acute toxicity when used for cosmetic purposes by performing an eye irritation test. new zealand white (nzw) rabbits were treated with a 100 mg/dose of s-(-)-10,11-dihydroxyfarnesic acid methyl ester according to standard procedure guidelines. no significant changes in terms of ocular lesions of the cornea, turbidity of the cornea, swelling of ... | 2013 | 24137288 |
| pathogenicity of beauveria bassiana isolated from moroccan argan forests soil against larvae of ceratitis capitata (diptera: tephritidae) in laboratory conditions. | the mediterranean fruit fly, ceratitis capitata wiedemann (diptera: tephritidae), is the major tephritid pest in morocco. this pest survives in moroccan forests argania spinosa and continually invades the nearest agricultural areas. entomopathogenic fungi are an interesting tool for fruit fly control and hold a useful alternative to conventional insecticides. however, primary selection of effective pathogens should be taken in laboratory condition prior to applying them in the field. here, we us ... | 2014 | 24122125 |
| entomopathogenic fungi in cornfields and their potential to manage larval western corn rootworm diabrotica virgifera virgifera. | entomopathogenic ascomycete fungi are ubiquitous in soil and on phylloplanes, and are important natural enemies of many soil-borne arthropods including larval western corn rootworm, diabrotica virgifera virgifera, which is a major pest of corn. we measured the prevalence of beauveria bassiana and metarhizium anisopliae sensu lato in ten cornfields in iowa, usa by baiting with larval insects. b. bassiana and m. anisopliae s.l. were present in 60% ± 6.3% and 55% ± 6.4% of soil samples, respectivel ... | 2013 | 24120889 |
| synthesis and bioconversions of formestane. | in an effort to generate new steroidal aromatase inhibitors, formestane (4-hydroxyandrost-4-ene-3,17-dione) (1) was biotransformed by rhizopus oryzae to yield the known 4β,5α-dihydroxyandrostane-3,17-dione as the major product (5) and bioconverted by beauveria bassiana to afford the known reduced 4,17β-dihydroxyandrost-4-en-3-one (6) and 3α,17β-dihydroxy-5β-androstan-4-one (7) and the new 4,11α,17β-trihydroxyandrost-4-en-3-one (8). all the metabolites showed more potent activities than their par ... | 2013 | 24074257 |
| beauveria bassiana (ascomycota: hypocreales) wound dressing for the control of euzophera pinguis (lepidoptera: pyralidae). | injury to olive tree trunks and branches because of biotic and abiotic factors, such as pruning and mechanical harvesting, attracts the olive pyralid moth euzophera pinguis haworth (lepidoptera: pyralidae). this moth has become increasingly important in the mediterranean region during recent years. the use of an entomopathogenic fungus for wound dressing for pest control is reported for the first time in this study. beauveria bassiana (ascomycota: hypocreales) strain eabb 08/04-ep was originally ... | 2013 | 24020271 |