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[clinical examination of the blood of roe deer (capreolus capreolus l.) and fallow deer (dama dama l.) naturally invaded by parasites].roe-deer (capreolus capreolus l. -- five animals) and fallow deer (dama dama l. -- eleven animals) of both sexes and at different age were subject to blood examination. the deer were killed in several preserves in bohemia during autumn and winter shootings in two years. the following blood values were determined: erythrocyte count, leucocyte count, hemoglobin content, hematocrit values, mcv, mch, mchc, white blood picture, total protein, its fractions (albumin, alpha-, beta-, and gamma-globulin) ...1975809892
susceptibilities of gram-negative bacteria to combinations of antimicrobial agents in vitro.combinations of either clindamycin or erythromycin with gentamicin, colimycin, or carbenicillin were studied for their antimicrobial effects on clinical isolates of escherichia coli, klebsiella sp., proteus mirabilis, serratia marcescens, enterobacter aerogenes, and pseudomonas aeruginosa. no antagonistic effects of the antibiotic combinations were observed. synergistic effects of the antibiotic combinations were noted against most strains of e. coli tested, especially when either clindamycin or ...1975810077
a very rapidly migrating f1 histone associated with gene-sized pieces of dna in the macronucleus of oxytricha sp.histones bound to gene-sized pieces of dna in the macronucleus of oxytricha sp. consist of five main fractions: f1, f2a1, f2a2, f2b and f3. although the slightly lysine-rich and the arginine-rich histones in oxytricha macronuclei are similar to comparable fractions in vertebrates, the lysine-rich f1 fraction differs. oxytricha f1 is unique among eukaryotes in that it migrates faster than f2a1 and is located nearest the cathode in polyacrylamide-urea gels.1975810173
the amino acid sequence of cerexin a (studies on antibiotics from the genus bacillus. vii.n-bromosuccinimide cleavage reaction on cerexin a liberated allo-isoleucine. treatment with conc. hydrochloric acid cleaved the antibiotic into two peptide fragments selectively at gamma-hydroxylysine residue. deacylation with an enzyme preparation from pseudomonas sp. afforded deacyl cerexin a. the amino acid sequences of these peptide fragments were examined by edman degradation. from all the results, the entire amino acid sequence of cerexin a was deduced.1975810467
interhemispheric functional differences in prefrontal cortex of monkeys.monkeys had nonpolarizable electrodes implanted bilaterally in prefrontal (principal sulcus), precentral, and occipital cortex. they were trained on a spatial delayed-response (dr) task (8-sec intratrial delay), while cortical potentials were recorded. three groups of monkeys were trained to 90% criterion: (a) 4 monkeys with only the right hand (the left wrist was attached to the testing chair); (b) 2 monkeys with only the left hand; and (c) 2 monkeys with the left and right hands on alternate s ...1975810542
light and electron microscopic observations of the life cycle of sarcocystis orientalis sp. n. in the rat (rattus norvegicus) and the malaysian reticulated python (python reticulatus).a light and electron microscopic study of sarcocystis orientalis sp. n. was made. the life cycle of this parasite is in two hosts. gametogony is in the intestinal epithelial cells of a predator, python reticulatus. isospora-like oocysts developed. sporocysts average 9.1 by 7.7 mum. rats (rattus norvegicus) were infected with sporocysts and asexual stages developed. ten days after infection large zoites (average 7.85 by 2.48 mum) were observed free in peripheral blood and within white blood cells ...1975810990
scanning electron microscopy of fruiting body formation by myxobacteria.scanning electron microscopy was used to follow fruiting body formation by pure cultures of chondromyces crocatus m38 and stigmatella aurantica. vegetative cells were grown on sp agar and then transferred to bonner salts agar for fructification. fruiting in both species commences with the formation of aggregation centers which resemble a fried egg in appearance. in chondromyces the elevated center or "yolk" region of the aggregation enlarges into a bulbous structure under which the stalk forms a ...1975811649
rumen ciliates of the tsessebe (damaliscus lunatus lunatus) in south africa.ciliate protozoa from the rumen of the tsessebe, damaliscus lunatus lunatus, all belong to the family ophryoscolescidae and no holotrichs were found in any of the 16 animals. eighteen species were identified of which entodinium fyferi sp. n., enoploplastron garstangi sp. n. and epidinium lunatus sp. n. are new and have been described for the first time. a short redescription of eremoplastron gigantium kofoid & maclennan is given.1975811788
encephalitozoon sp. in the blue-masked lovebird, agapornis personata (reichenow): first confirmed report of microsporidan infection in birds.a microsporidan tentatively classified as encephalitozoon sp. on the basis of structure and tinctorial qualities was identified in tissues from a group of blue-masked lovebirds. agapornis personata (reichenow). the organism was present in renal tubular epithelial cells, hepatocytes, bile duct epithelial cells, and intestinal epithelial cells. focal hepatic necrosis occurred around clusters of the organisms but inflammation was minimal or absent in the other infected tissues. attempts to infect l ...1975811790
hepatozoon sp. infection in mink from southwestern ontario.schizonts of hepatozoon sp. were found in the lungs of 10 of 18 (56%) mink (mustela vison). schizonts were located in microgranulomas within the pulmonary parenchyma, occasionally in peribronchiolar or perivascular sites.1975811814
hemoprotozoa in mourning doves and other small birds of western oklahoma.blood smears obtained from 370 birds live-trapped in western oklahoma were examined for hemoprotozoa. haemoproteus spp. were found in 189 (90.4%) mourning doves (zenaida macroura), one oriole (icterus galbula), two mockingbirds (mimus polyglottos), and three brown thrashers (toxostoma rufum). plasmodium sp. was present in one brown thrasher. haemoproteus spp. in the mourning dove were identified as h. sacharovi and h. maccallumi, with the latter species predominating. the average parasitemia for ...1975811816
an analytical study of the role of various factors causing red tide outbreaks of trichodesmium as deduced from field and laboratory observation.the marine blue green alga trichodesmium sp is found in red-tide outbreaks in sub-tropical areas in the pacific and indian oceans, the arabian seas and the equatorial currents of the gulf of mexico. giberellic acid stimulated the growth of trichodesmium cultures to a greater extent than in the diatom melosira sulcata. while increasing either the nitrate or phosphate above the normal levels in the culture medium did not greatly stimulate the growth of this organism, trichodesmium did thrive in ni ...1975812691
nosema slovaca sp. n.: a second microsporidian of the tick ixodes ricinus. 1975812923
[etiology of dairy cows mastitis in slovakia in the years 1972-1974].in the period from 1972 to 1974, veterinary institutes in slovakia performed bacteriological examinations in 178,853 milk samples coming from 72,454 dairy cows. on an annual average, the number of examined samples were higher by 61% than in 1967-1971. germs responsible for the inflammation of the mammary gland were found in the milk of 22.32% of the dairy cows. the following bacterial germs were represented: streptococcus agalactiae -- 15.77%, staphylococcus aureus -- 4.19%, other streptococci ...1975813355
an unusual mouse myeloma protein binding native dna.sp 104 is an iga-kappa myeloma protein produced by a lymphoid tumour of ca f1 mice. it arose originally in mice injected intraperitoneally with cell-free extract of spleen from a dog with systemic lupus erythematosus. the monoclonal nature of the iga was shown by characteristic appearance on immunoelectrophoresis, restriction to a single light chain type and ability to induce anti-idiotypic antiserum. this protein has antibody activity against native (double-stranded) dna and its specificity is ...1975813922
[etiology of mastitis in dairy cows in slovakia during 1972-1974].in the period from 1972 to 1974, veterinary institutes in slovakia performed bacteriological examinations in 178,853 milk samples coming from 72,454 dairy cows. on an annual average, the number of examined samples was higher by 61% than in 1967-1971. germs responsible for the inflammation of the mammary gland were found in the milk of 22.32% of the dairy cows. the following bacterial germs were represented: streptococcus agalactiae - 15.77%, staphylococcus aureus - 4.19%, other streptococci - 1. ...1975814671
[reproductive capacity and milk production in cows of various cattle breeds as related to age at the ist calving].the course of parturition, the ability to get in-calf, and milk production were studied in 348 first-calved cows, in 162 cows of the bohemian spotted breed, 88 cows of the black-pied lowland breed, and 98 crossbreds of these two breeds divided into three categories according to the age at the first calving (from 21 to 23 months, 24 months, and from 25 to 29 months). there were only slight differences in the average calf birth weight between the cows of all the breeds calving for the first time b ...1975814676
[infectious pancreatic necrosis virus: acquired susceptibility to trout serum neutralizing factor after repeated passage in cell culture].it has been shown previously that in normal sera from ipn free rainbow trout a "6 s molecule" neutralized sp type ipn viruses routinely used in european fish diseases laboratories. these viruses were unable to reproduce the disease under experimental conditions. after a survey of trout farms, a sp type pathogenic virus was isolated (isolate 31/75). after one passage on rtg2 cells this virus was fed to 7 weeks old fry and led to a 70% mortality. in contrast, a routinely passed and cloned virus (2 ...1975815032
prevalence of sarcocystis infection and other intestinal parasitisms in cats from a humane shelter in ohio.feces of 1,000 cats from a humane shelter in columbus, ohio were examined for evidence of intestinal parasitisms. prevalences (in percentage of oocysts or helminth eggs) were: sarcocytis sp, 0.2; toxoplasma-like sp, 1.0; isospora felis, 6.2; isopora rivolta, 3.2; toxocara cati, 25; capillaria sp, 1.3; ancylostoma sp, 9.4; uncinaria sp, 0.2; and taenia sp, 0.5. infectivity of coccidia found in feces was tested by oral inoculation into mice and by feeding inoculated mice to cats. toxoplasma-like o ...1976815229
prevalence of sarcocystis infection and other intestinal parasitisms in dogs from a humane shelter in ohio.fecal specimens from 500 stray dogs in columbus, oh, were examined for evidence of intestinal parasitisms. prevalences (%) of coccidia were: sarcocystis sp, 1.8; isospora canis, 1.8; and isospora ohioensis, 3.6. prevalences (%) of helminths were: toxocara canis, 19.2; toxascaris leonina, 10.2; trichuris sp, 42.2; capillaria sp; 1.0; ancylostoma sp, 60.8; uncinaria sp, 2.4; and taenia sp, 7.4.1976815230
in vitro synthesis of the unit that links teichoic acid to peptidoglycan.the role of cytidine diphosphate (cdp)-glycerol in gram-positive bacteria whose walls lack poly(glycerol phosphate) was investigated. membrane preparations from staphylococcus aureus h, bacillus subtilis w23, and micrococcus sp. 2102 catalyzed the incorporation of glycerol phosphate residues from radioactive cdp-glycerol into a water-soluble polymer. in toluenized cells of micrococcus sp. 2102, some of this product became linked to the wall. in each case, maximum incorporation of glycerol phosph ...1976815251
microbial metabolism of polychlorinated biphenyls. studies on the relative degradability of polychlorinated biphenyl components by alkaligenes sp. 1976815303
biotransformation of antibiotics. i. acylation of chloramphenicol by spores of streptomyces griseus isolated from the egyptian soil .incubation of spores, washed mycelium or whole cultures of a streptomyces sp. with chloramphenicol (i) resulted in the loss of in vitro bioactivity of the antibiotic. gas chromatographic estimation of an appropriate extract revealed that more than 95% of the antibiotic was inactivated under the specified conditions. the spores inactivated chloramphenicol in an inorganic buffer solution, or in distilled water, without the addition of carbohydrate or external co-factor. however, addition of certai ...1976816764
the intestinal parasites of a community of feral chimpanzees, pan troglodytes schweinfurthii.fecal specimens from 32 champanzees living in gombe national park, tanzania, were examined. six species of helminths and 2 species of ciliates were found: probstmayris gombensis file (in press), strongyloides fuelleborni von linstow 1905, necator sp., oesophagostomum sp., abbreviata caucasica von linstow 1902, trichuris sp., troglodytella abrassarti brumpt and joyeux 1921, and an unidentified ciliate. none of the parasitic infections were heavy. this is the first such survey of the chimpanzee in ...1976817011
[nematodes of the genus spirura, parasites of tupaia and nycticebus in malaysia (author's transl)].morphological study of two spirura parasites of the oesophageal and the gastric wall of tupaia and nycticebus in malaysia. -- spirura malayensis n. sp. is found both in tupaia in the district of selangor (west malaysia) and in nycticebus coucang in borneo. its very primitive characteristics relate it to s. diplocyphos chabaud, brygoo and petter, 1965, parasite of lemurs from madagascar. its larval development was obtained experimentally in blatella germanica. -- spirura aurangabadensis (ali and ...1975818936
observations on haemogregarina balli sp. n. from the common snapping turtle, chelydra serpentina.haemogregarina balli sp. n. is described from the blood and organs of the common snapping turtle chelydra serpentina serpentina and from the gastric and intestinal ceca of the presumed invertebrate hosts, the leeches placobdella parasitica and placobdella ornata. in the peripheral blood of the turtle, male and female gametocytes and immature erythrocytic schizonts are found within erythrocytes. the maturation of erythrocytic schizonts containing 6-8 merozoites is recorded from liver imprints. sc ...1976819649
anthrax in captive carnivores in ibadan, nigeria.between 20 and 22 june 1974, three captive carnivores (two genets, genetta sp. and one caracal, felis caracal) died suddenly in the university of ibadan zoological garden without previous signs of illness. bacillus anthracis was isolated from their blood and tissues. the organism was highly pathogenic to laboratory animals. the disease was believed to have been contracted from meat fed the animals.1976819662
a study of the feasibility of the use of gentian violet as a fungistat for poultry feed.a 96% formulation of gentian violet was fungistatic to aspergillus flavus, alternaria sp., candida albicans, fusarium moniliforme, and penicillum citrinum when incorporated into corn meal agar at 6.5, 39.0, and 156.0 p.p.m. a. flavus, alternaria sp., f. roseeum, and p. citrinum were inhibited on corn meal agar which had been amended with 12.8, 26.6, and 38.4 p.p.m. of gentian violet in a 2.54% commercial liquid concentrate of gentian violet employed as the active ingredient in a commercial poult ...1976819918
cyanobacterial dna-binding protein related to escherichia coli hu.a dna-binding protein has been isolated from two cyanobacteria (blue-green algae): anabaena sp and aphanocapsa sp. it has a molecular weight as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis of about 10,000, is rich in lysine and arginine, and lacks tyrosine, tryptophan, and cysteine. the proteins from both strains show immunological identity with a similar dna-binding protein from escherichia coli, when tested by immunodiffusion with an antiserum prepared against the e. ...1976819928
role of the micropore in nutrition of the sporozoa. ultrastructural study of plasmodium cathemerium, eimeria ferrisi, e. stiedai, besnoitia jellisoni, and frenkelia sp. 1976821187
[thermosensitive sporulation and extracellular serylprotease mutant of b. subtilis].isolation and properties of b. subtilis ts 19 mutant, isolated as thermosensitive for sporulation, are described. at the non permissive temperature (42degreesc), the mutant cells are blocked at stage zero of sporulation and do not excrete extracellular enzymes such as serylprotease and esterase. at the permissive temperature (30degreesc), sporulation and excretion of extracellular enzymes are normal but the serylprotease is modified in its structure. two molecular forms of this enzyme can be sep ...1976821540
facultative wood-digesting bacteria from the hind-gut of the termite reticulitermes hesperus.among the facultative bacteria capable of growth on mesquite wood which were isolated from the asceptically dissected hind-gut of the termite reticulitermes hesperus were two strains of bacillus cereus, one strain each of arthrobacter, alcaligenes and serratia, and a very small gram-negative fermentative rod. the b. cereus strains, the serratia marcescens strain and the arthrobacter sp. grew well on a mineral salts alpha-cellusose agar. one of the bacillus cereus strains and serratia marcescens ...1976822127
nonidentical subunits of protocatechuate 3,4-dioxygenase.protocatechuate 3,4-dioxygenase (ec 1.13.11.3) has been reported to have a molecular weight of 700,000 and to consist of eight identical subunits, each containing one atom of ferric iron and a substrate binding site. this subunit has now been found to dissociate further into four smaller subunits of two nonidentical types (alpha2beta2), upon sodium dodecyl sulfate gel electrophoresis. the molecular weights of the alpha and beta subunits were estimated to be 22,500 and 25,000, respectively. isoel ...1976822864
autochton blue-green alga (oscillatoria sp.) with high protein content and self-aggregation properties. 1976822900
[intestinal coccidiosis of the rabbitt: comparison of the pathogenic power of eimeria intestinalis with 3 other eimeria].the study of the specific pathogenicity of eimeria sp. was impossible in the absence of coccidium free rabbits and in consequence of pure strains of coccidia. recently, we could realise these two conditions and started the comparative study of e. magna, e. media, e. perforans and e. intestinalis infection. e. intestinalis is characterised by a higher pathogenicity: symptoms and mortality are more important and complete recovery is rare.1976822959
[importance of the fox (vulpes vulpes) in the transmission of sarcosporidiosis from the steinbock (capra ibex) at the gran paradiso national park].sarcosporidiasis is a very common infection of steinbocks (capra ibex) and chamois (rupicapra rupicapra) of gran paradiso national park, italian western alps, frequently with serious cardiac involvement. no dogs or cats are allowed inside the park, to the contrary foxes (vulpes vulpes) are present every where, near steinbocks and chamois, and eat on the bodies of dead animals. feces of vulpes vulpes were collected in the same area, where the infected capra ibex used for the following experiment ...1975823518
influences of copper and design of intrauterine devices on endometrial bleeding in monkeys.uterine bleeding in 8 rhesus monkeys was compared in response to plain vs. a specially fitted copper-wrapped iud. all animals had utero-cutaneous surgical fistulas for accurate iud insertion and removal. daily observations for uterine bleeding between menstruations and quantitation of menstrual blood loss (mbl) were made. following control periods of observation, each monkey was inserted with either a plain or a copper-wrapped iud and then studied for 4 consecutive menstrual periods. each iu ...1976824096
purification and partial characterization of human pancreatic elastase.1) an elastolytic enzyme has been isolated from human pancreatic juice. the concentration of the enzyme was about 0.6 mg/ml corresponding to about 10% of the protein concentration of the juice. 2) the purification procedure included chromatography on sephadex g-25 followed by ion-exchange chromatography on sp-sephadex c-50 at ph 6.0, affinity chromatography on trasylol-sepharose 4-b and a final ion-exchange chromatography on sp-sephadex at ph 7.6. the yield was about 50%. 3) the elastase isolate ...1976824194
a review of sarcocystis of domestic animals and of other coccidia of cats and dogs.the nomenclature, life cycles, and pathogenicity of sarcocystis of domestic animals are reviewed. sarcocystis had a 2-host life cycle, with carnivores as definitive hosts and herbivores as intermediate hosts. the following species are found in domestic animals (with the definitive hosts given in parentheses): 3 species in the ox: s cruzi (dog, wolf, coyote, raccoon, fox), s hirsuta (cat), s hominis (man, monkey); 2 species in the sheep: s ovicanis (dog), s tenella (cat); 3 species in the pig: s ...1976824260
derepressed synthesis of cellulase by cellulomonas.a cellulomonas sp. was isolated from the soil which hydrolyzed cellulose, as shown by clear-zone formation on cellulose agar medium. catabolite repression of cellulase synthesis occurred when moderate levels of glucose were added to the medium. a stable mutant that no longer exhibits catabolite repression was produced through treatment of the wild-type organism with n-methyl-n'-nitro-n-nitrosoguanidine. both enzyme concentration and specific activity, as determined by the rate of hydrolysis of c ...1976824282
growth and nitrogen fixation of mutants defective in heterocyst differentiation in the blue-green alga cylindrospermum sp.three classes of spontaneous and n-methyl-n'-nitro-n-nitrosoguanidine (ntg)-induced mutants of the blue-green alga cylindrospermum sp. were isolated in the presence of combined nitrogen. mutants with complete loss of heterocyst differentiation formed longer filaments than the parent alga and were unable to grow in nitrogen-free medium and to fix nitrogen. the second type of mutants formed long filaments with terminal heterocysts. in the third type of mutants, paired proheterocyst differentiation ...1976824870
[production and characteristic constituents of the algae spirulina platensis and maxima].microscopic blue-green spirulina algae, such as have been consumed since ancient times, are algae having a very high protein content (70 p. 100 of dry matter). at present the spirulina platensis species in the kanem region in chad is gathered by primitive methods, sold in the marketplace and consumed by the local population. the spirulina maxima species exists in a seminatural state in mexico city, and an industrial pilot plant harvests and produces one ton per day. both species, sp. platensis a ...1975824992
virus and bacteria removal from wastewater by land treatment.secondary sewage effluent and renovated water from four wells at the flushing meadows wastewater renovation project near phoenix, arizona, in operation since 1967, were assayed approximately every 2 months in 1974 for viruses and enteric bacteria during flooding periods. no viruses of salmonella sp. were detected in any renovated well water samples, and the numbers of fecal coliforms, fecal streptococci, and total bacteria were decreased by about 99.9% in the renovated well waters after the wast ...1976825040
[action of synthetic substance p (sp) on the respiration, general metabolism and systemic hemodynamics of the anesthetized dog].in the anaesthetized dog, synthetic substance p in one dose (0.5 mug/kg i.v.) induced a quick hypotensive but short-lasting action and a respiratory analeptic activity which only appeared with some delay and lasted more than one hour. the possibility of a rapid metabolization of synthetic sp is discussed.1976825246
[inhibition of proteus sp. by nalidixic acid].the investigation comprised 192-positive and gram-negative strains of bacteria, including 111 proteus sp. the degree of resistance of these strains to nalidixic-acid was investigated on blood agar with 25 mug, 40 mug, and 50 mug nalidixin-acid per ml medium. none of the proteus strains were able to grow on medium with 50 mug nalidixic-acid per ml, whereas streptococcus sp., corynebacterium pyogenes, peptococcus indolicus, pseudomonas aeruginosa, and beta-toxic staphylococci all were found resist ...1976825831
[peptidases of preparations of proteolytic enzymes from several species of bacteria, molds and actinomycetes].the activity of carboxy- and aminopeptidases of enzymic preparations obtained from bacteria bacillus subtilis 1 m, 17, bacillus mesentericus 100/26, 100/56, 16; pseudomonas aeruginosa 1/7a, 1/4; bacillus thermophilicus s. sp. nov b-22, moulds aspergillus oryzae 1-746, aspergillus flavus 716 and actinomycete streptomyces griseus 32-13 was studied. in the bacterial preparations the proteolytic activity was 1.5-2.5 times lower than in the fungal and actinomycete preparations. the carboxypeptidase a ...1976825850
[action of various beta(1-3)-d-glucanases on the wall of yeasts: taxonomic applications].purified beta-(1-3)-d-glucanases (from aspergillus nidulans, badidiomycetes sp. qm 806, trichoderma viride) are used to release protoplasts from various yeasts. two of them may prove taxonomic correlation. the enzyme of basidomycetes sp. releases protoplasts from ascomycetes, the enzyme of t. viride releases protoplasts from ascomycetes and heterobasidiomycetes; none of them acts on the basidiomycetes.1976826331
blood parasites of taiwan birds.blood films were examined from 1477 birds of taiwan (193 species, 49 families). haemoproteus kruse was by far the commonest parasite, with leucocytozoon danilewski a not very close second. it is probable that some of the haemoproteus infections represented new species, and 1 occurring in the bamboo partridge (bambusicola thoracica sonorivox gould) seemed characteristic enough to justify recognition as such; the name haemoproteus bambusicolae sp. n. is proposed for this organism. malaria was foun ...1976826624
[isolation of aspergillus sp. strains producing aflatoxins from food for human consumption in mexico]. 1976827002
[antifertility effect of an active immunization of monkeys with human pregnancy-specific beta 1-glycoprotein (sp1) (author's transl)].fertile female cynomolgus monkeys (macaca fascicularis) were actively immunized against human pregnancy-specific beta 1-glycoprotein (sp1). native sp1 as well as chemically modified derivatives of sp1 served as antigens. the effect of the immunization on reproduction in these animals was investigated. the immunized monkeys showed a significant reduction in fertility: the conception rate in these animals was only slightly reduced when compared with the controls; but in the immunized monkeys pregn ...1976827272
oxidation of methanol by facultative and obligate methylotrophs.1. the newly isolated methanol obligate methylomonas sp. and the methanol facultative pseudomonas sp. oxidize methanol at an unchanged rate over concentration range from 0.1 to 600 mm; the oxidation rate by the obligate methylotroph is 2.5 times higher (300 nmoles o2/min/mg dry wt.). low-molecular alcohols, formaldehyde and formate serve as respiratory substrates for the intact cells of both methylotrophs. 2. methanol dehydrogenase of both methylotrophs isolated should be classified as the phena ...1976827889
[on the acute respiratory infections of anaerobes bacteria: clinical, bacteriological and therapeutic results (author's transl)].in order to isolate and identify aerobe and obligate anaerobe (o.a.) bacteria investigations were carried out on 11 patients with acute respiratory tract infections (7 of them severely ill and hospitalized in an intensive care unit). o.a. species were isolated in 7 subjects, in all cases in mixed culture (from 2 to 5 species per sample), including also potentially pathogenic aerobe bacteria (pseudomonas aeruginosa, klebsiella pneumoniae, etc). on the 24 strains of o.a. bacteria identified, m.i.c ...1976827988
characterization and structural properties of the major biliproteins of anabaena sp.studies are presented of the biliproteins of anabaena sp. this filamentous cyanobacterium contains three major biliproteins. whereas two of these, c-phycocyanin and allophycocyanin, are common to all cyanobacteria, the third, phycoerythrocyanin (gammamax approximately 568 nm) has hitherto not been described and its distribution among cyanobacteria appears to be limited. anabaena variabilis and anabaena sp. 6411 allophycocyanin, c-phycocyanin, and phycoerythrocyanin were purified to homogeneity a ...1976828020
the fine structure of a microplate-microtubule array, microfilaments and polyhedral body associated microtubules in several species of anabaena.a microplate-microtubule array was observed in anabaena sp. (b-378). this structure consists of an arched plate, about 8 nm thick, and various microtubules, 12 nm in diameter and 50 nm long, arranged in rows. the microtubules project at right angles from one side of the plate into the cytoplasm or towards the plasma membrane. up to twelve microplate-microtubule arrays were observed in a single section of a cell. microfilaments, about 2.8 nm in diameter and of undetermined length, were observed i ...1976828027
protozoan parasites of carp (cyprinus carpio l.): a comparative study of their occurrence in bulgaria and czechoslovakia, with the description of trichodina perforata sp.n.in the course of a long-term study of protozoan parasites of carp (cyprinus carpio l.), a total of 27 protozoan species were discovered in czechoslovakia and 26 species on the bulgarian territory. their list is supplemented with remarks on their pathogenicity, frequency of occurrence and morphology. there is no essential difference in the constituents of protozoan parasitofauna of carp between the two territories studied; a very similar fauna of protozoans can be anticipated on carp in other eur ...1976828131
pneumocystis jiroveci n. sp. from man: morphology, physiology, and immunology in relation to pathology.pneumocystis jiroveci n. sp. from humans is redescribed, including the trophozoite precyst, cyst, intracystic bodies, and empty cyst. the morphology, biology, and physiology of this organism are compared with those of p. carinii from rats. drug-induced immunosuppression produces similar disease patterns in both hosts, but several other pathogenic mechanisms seen in man have not been reproduced in rats. in most humans, immunity terminates infection and carriers are few; but in rats, immunity is g ...1976828240
mesocestoides in the baboon and its development in laboratory animals.tetrathyridia of mesocestoides sp. were recovered from three wild-captured african baboons. active larvae were administered orally to laboratory-reared dogs, cats, rats and mice. development in canine hosts was rapid and a high percentage of mature intact worms was recovered as early as 14 days post-exposure. the few worms recovered from the feline hosts were stunted and fragmented. no adult worm development occurred in the rats or mice. no significant clinical or pathological manifestations wer ...1976828931
[the effect of dehelminthizations performed during the year on the seasonal dynamics of natural nematode infections in sheep].in four sheep flocks of two age categories dynamics of natural infections by pulmonary and gastrointestinal nematodes was studied; sheep were kept on a farm in western bohemia. dehelminthizations were performed in different intervals during the grazing period on the basis of the results of quantitative coprologic examinations. total effectiveness of 80--100% intenseffectiveness (ie) was obtained as a result of single peroral or intraruminal dehelminthization with the following preparations: pyra ...1976828996
[etiology of mastitidos in dairy cows in slovakia during 1975].in 1975, in the veterinary institutes in slovakia bacteriological examinations of 54824 samples of milk coming from 28737 dairy cows were performed. bacterial germs responsible for the inflammation of the mammary gland were found in the milk of 18.91% of cows. these were: streptococcus agalactiae (in 13.93% of cows), staphylococcus aureus (2.86%), other streptococci (1.32%), e. coli (0.13%), klebsiella sp. (0.13%), corynebacterium pyogenes (0.15%), other bacterial germs (0.39%). streptococci and ...1976828997
quantitative microflora of the vagina.this study enumerated the predominant vaginal flora in 25 healthy patients: 18 prenatal (six with and 12 without gonorrhea) and seven nonpregnant without gonorrhea. all 25 patients had aerobes and 19 of the 25 had anerobes isolated in titers of greater than or equal to 10(5) colony-forming units (cfu per milliliter). the most frequently isolated microorganism was the lactobacillus (aerobic and anaerobic species) followed by c. vaginale, viridans streptococci, anaerobic gram-positive cocci, and b ...1977831488
mycoplasma agalactiae subsp. bovis in pneumonia and arthritis of the bovine.the pneumonic lungs of 42 cattle from 26 feedlots were examined for the presence of mycoplasma, pathogenic bacteria and viruses. four animals representative of two lots failed to yield mycoplasma. one of these yielded the virus of infectious bovine rhinotracheitis and pasteurella hemolytica, the other yielded only p. p. multocida. nine animals in eight lots yielded mycoplasma sp.: five of these were m. bovirhinis, two were m. arginini and two were untypable. all of these animals yielded one or m ...1977832194
bacteriological evaluation of fosfomycin in clinical studies.since fosfomycin has behaved in vitro as a broad-spectrum antibiotic, an attempt has been made to evaluate this behaviour in controlled clinical study carried out at different spanish hospitals. a total of 959 patients were treated for some of the following infectious clinical processes: gonococcal urethritis, typhoid fever, enterocolitis, acute and chronic urinary tract infections, osteomyelitis, chronic otorrhoea, septicaemia, meningitis, peritonitis, surgical and suppurative infections, bronc ...1977832523
fosfomycin in infantile acute gastroenteritis.a clinical test was carried out on 83 children suffering from infectious diarrhoeal processes who were treated with fosfomycin with the oral and paranteral route. coproculture was carried out in all the children before the beginning of treatment and in 51 of them it was repeated at the end of treatment. antibiograms were made on the isolated germs and their mic to fosfomycin studied. tolerance and secondary effects of the medicament were also studied. the study was considered insufficient in 13 ...1977832529
clinical bacteriological evaluation of infantile bacteriuria treated with fosfomycin.the therapeutic action of fosfomycin has been studied in a group of 14 children, with simple and complicated infections of the urinary tract with congenital renal malformations. in the bacteriological study of the urine it was observed that the strains most frequently isolated were e. coli, proteus sp. and klebsiella sp. of the 25 strains isolated 76% were sensitive to the disc of 50 mug fosfomycin and 24% moderately sensitive with a mic of 64 and 128 mug/ml. the doses of fosfomycin used were 15 ...1977832532
activity of fosfomycin in the treatment of bacterial infections.30 patients with different infections were treated with fosfomycin: 13 had urinary infections, 14 had pneumonial infections, 2 had staphylococcus osteomyelitis and 1 had staphylococcus septicemia. the antibiotic was administered in doses ranging from 100 to 230 mg/kg/day, with periods of treatment that lasted from 5 to 58 days. the doses were administered every 6 h by the oral or intramuscular route. a total of 35 organisms were isolated: 7 e. coli, 7beta-hemolitic streptococcus, 6 proteus sp., ...1977832537
selective immunoglobulin m deficiency in foals.selective immunoglobulin m deficiency was diagnosed in 5 foals, on the basis of reduced serum immunoglobulin m concentrations (more than 2 standard deviations below the normal mean). all 5 foals had clinical signs or lesions involving the respiratory tract. lkebsiella sp was isolated from joint fluids, bronchial lymph nodes, or tracheal washings from the 3 foals in which such samples were available for microbiologic evaluation.1977833046
isolation and identification of fecal bacteria from adult swine.an examination of the fecal microflora of adult swine was made with regard to the efficiency of several roll tube media in enumeration and recovery of anaerobes, the effects of medium constituents on recovery, and the isolation and identification of the predominant kinds of bacteria. total number of organisms by microscopic bacterial counts varied among fecal samples from 4.48 x 10(10) to 7.40 x 10(10) bacteria/g (wet weight). comparison of different nonselective roll tube media indicated that a ...1977836025
nmr characterization of three forms of ferredoxin from desulphovibrio gigas, a sulphate reducer.a nmr and magnetic susceptibility study of the oxidized and reduced states of three different oligomers (forms) of a [4fe-4s] ferrodoxin protein from desulphovibrio gigas, fdi, fdi', and fdii was carried out. fdi and fdi' are different trimers and fdii a tetramer of the same basic subunit. a probable assignment of the contact shifted resonances is indicated. since the temperature dependences of the contact shifted responances associated with each [4fe-4s] are not all similar a delocalized model ...1977836818
inhibition of virus-induced plaque formation by atoxic derivatives of purified cobra neurotoxins.venom from naja naja siamensis was resolved into 16 toxic and nontoxic fractions by chromatography on sp-sephadex, c-25. the principal neurotoxin preparations were chromatographically and electrophoretically homogeneous. of the purified constituents, only the principal neurotoxin and minor neurotoxins were precursors of inhibitors of plaque formation among baby hamster kidney fibroblasts infected with semliki forest virus.1977836894
dna content in the genus xenopus.nuclear dna amounts were determined by cytofluorometry for twelve species and subspecies of the genus xenopus. absolute values, in pg per nucleus, were obtained by direct comparison with human lymphocyte nuclei. the lowest dna amount (3.55 pg) was found in x. tropicalis, which possess only 20 chromosomes, and the highest (16.25 pg), in the hexaploid x. ruwenzoriensis, with 108 chromosomes. the two recently discovered tetraploid species, x. sp.n. and x. vestitus have, respectively 12,57 and 12.83 ...1977837803
the solid phase synthesis of porcine secretin with full biological activity.the solid phase synthesis of procine secretin is described. the c-terminal residue was attached to a polymeric amino support and all the boc-amino acids, including boc-glutamine, were coupled by a modified carbodiimide method. a preliminary test synthesis showed that the couplings of several amino acids of the n-terminal section were unsatisfactory. this problem was overcome in the main synthesis by executing all the major reactions twice. cleavage of the peptide fromthe resin as well as the rem ...1977838532
isolation and characterization of a new nucleoside antibiotic, amipurimycin.a new antibiotic amipurimycin, active against pyricularia oryzae in vitro and in vivo, was isolated from the culture filtrate of streptomyces novoguineensis nov. sp. the antibiotic was purified by a combination of ion-exchange and adsorption chromatography based on its amphoteric water-soluble characteristics. its molecular formula was estimated to be c20h27approximately31n7o8-h2o. characteristic maxima in the uv spectrum and signals in the pmr and cmr spectra were similar to those of 2-aminopur ...1977838627
a new aminoglycoside antibiotic complex--the seldomycins. i. taxonomy, fermentation and antibacterial properties.a soil isolate named streptomyces hofunensis sp. nov. was found to produce seldomycin factors 1, 2, 3 and 5, new aminoglycoside antibiotics. taxonomy of the producing organism, a study of cultural conditions for seldomycin production, and antibacterial activity of seldomycins are reported. seldomycin factor 5 was the most active both in vitro and in vivo against gram-positive and negative bacteria.1977838628
a new aminoglycoside antibiotic complex--the seldomycins. ii. isolation, physicochemical and chromatographic properties.an antibiotic complex consisting of four components, seldomycin factors 1, 2, 3 and 5 was isolated from the fermentation broth of streptomyces hofunensis sp. nov. by use of a cationic exchange resin. after silica gel column chromatography, the purified components were characterized as new aminoglycoside antibiotics by their physicochemical, chromatographic and antimicrobial properties.1977838629
distribution of chloramphenicol acetyltransferase and chloramphenicol-3-acetate esterase among streptomyces and corynebacterium.chloramphenicol-3-acetate esterase activity was detected in cell-free extracts of strains of streptomyces venezuela, streptomyces sp. and streptosporangium viridogriseum var. kofuense which produced chloramphenicol and also corynebacterium hydrocarboclastus which produced chloramphenicol analogs (corynecins). none of the cell-free extracts of chloramphenicol- or corynecin-producing strains possessed chloramphenicol acetyltransferase activity under conditions which avoided the influenced of the e ...1977838634
modified sutter's arginine dihydrolase medium for pseudomonas speciation.sutter's arginine dihydrolase medium has been modified to obtain maximum yields of arginine dihydrolase. bromothymol blue is the indicator for alkalinity in sutter's medium. by adding glucose and lowering the ph of the medium, more positive reactions were obtained in 24 hr as well as a sharper color contrast to the base medium which facilitated reading the reactions. the modified sutter's medium was included in the routine biochemical schema for speciation of pseudomonads isolated from cosmetic ...1977838673
nitrogen fixation in nitrate reductase-deficient mutants of cultured rhizobia.forty-eight mutants unable to reduce nitrate were isolated from "cowpea" rhizobium sp. strain 32hl and examined for nitrogenase activity in culture. all but two of the mutants had nitrogenase activity comparable with the parental sttain and two nitrogenase-defective strains showed alterations in their symbiotic properties. one strain was unable to nodulate either macroptilium atropurpureum or vigna uguiculata and, with the other, nodules appeared promptly, but effective nitrogen fixation was del ...1977838684
determination of the configurations of lactic and glyceric acids from human serum and urine by capillary gas-liquid chromatography.the separation of the enantiomers of lactic and glyceric acids can be achieved by capillary gas chromatography on sp-1000 using the corresponding o-acetylated methyl esters. the structures of the derivatives were proved by proton magnetic resonance spectroscopy and mass spectrometry. the method has been used for the determination of the absolute configuration of lactic and glyceric acids isolated from serum and urine from different patients.1977838825
[the occurrence of neuraminidase and n-acylneuraminate pyruvate lyase in haemophilus paragallinarum and haemophilus paravium n.sp (author's transl)].14 strains of h. paragallinarum, isolated from fowls suffering from infectious coryza or received from other investigators for comparison, and a collective consisting of 9 strains of the new species h. paravium nova species, also isolated from fowls, were shown to possess both enzymes, neuraminidase (ec 3.2.1.18) and n-acylneuraminate-pyruvate lyase (ec 4.1.3.3). the detection of the enzymes was made initially by paper chromatography using n-acetyl-neuraminyllactose as substrate and secondly by ...1977842179
evolution of cm, sp and ap during etacrynic acid intoxication in the guinea pig.during etacrynic acid intoxication in the guinea pig, patterns of click-evoked compound electrocochleographic responses are similar to those observed in menière and retro-cochlear human pathologies. using high frequency tone bursts, sp, cm and ap were studied after intracardiac injection of etacrynic acid. the evolution of sp precedes that of cm and ap. its change in polarity could be related to that reported for ep by bosher et al. (1973). while during the recovery period sp overshoots (after 9 ...1977842314
effect of environmental temperature stress on intramammary infections of dairy cows and monitoring of body and intramammary temperatures by radiotelemetry.four dairy cows were stressed by exposure to hot and cold environments in tests to determine the effect of environment on milk yield, somatic cell counts, and california mastitis test scores of milk from all mammary quarters and on bacterial counts of milk from infected quarters. two cows were held in temperature-controlled rooms for successive 5-day periods at moderate (21 to 28 c), cold (-16 c), moderate, hot (36 to 37 c), and moderate environments. the cold and hot sequences were reversed for ...1977842915
rat bioassay for estimating toxicity of plant material from larkspur (delphinium sp).a technique was developed for estimating the toxicity for rats of an alcoholic extraction product from larkspur (delphinium barbeyi huth). the dried residue from alcohol extraction was reconstituted in buffered saline solution and subcutaneously injected into rats. toxicity was expressed as miligrams of dry plant per gram of body weight. samples from d barbeyi collected during prebloom and early flower bud stages of growth produced median lethal doses ranging from 5.0 to 6.7 mg/g of body weight. ...1977842926
ultrastructure of butyrivibrio fibrisolvens: a gram-positive bacterium.the cells of bacteria of the genus butyrivibrio are universally described as being gram negative, and they produce an unequivocal gram-negative reaction in the standard staining procedure. however, their cell walls contain derivatives of teichoic acid, which are characteristic of gram-positive cells. in this study, the cell walls of two representative strains of butyrivibrio were of the gram-positive morphological type, as seen by electron microscopy, but they were very thin (12 to 18 nm). the t ...1977845122
gyrodactylus plotosi n. sp. (trematoda: monogenea) from the silurid fish plotosus lineatus from the philippines. 1977847866
[susceptibility to thiamphenicol and chloramphenicol of anaerobic bacteria (author's transl)].the in vitro susceptibility to thiamphenicol and chloramphenicol of 272 anaerobes, most of which were recent clinical isolated, was determined by broth dilution tests, with chloramphenicol, 133 anaerobic gram-negative non-sporing rods (48 bacteroides fragilis, 13 b. thetaiotaomicron, 14 b. oralis, 16 sphaerophorus varius etc.) had mic values of 0.03 through 16 microng/ml. very similar results (mic, 0.06-16 microng/ml) were obtained with thiamphenicol. in concentrations of 4 microng/ml or less ch ...1977848218
pathogenesis and epidemiology of opportunistic mycotic infections: a review.the pathogenesis, epidemiology, and ecology of pathogenic and opportunistic systemic infecting fungi are reviewed. the pathogenic fungi including the etiologic agents of histoplasmosis, blastomycosis, coccidioidomycosis, and paracoccidioidomycosis are limited geographically, can establish an infection in a normal host, and exhibit thermal dimorphism. in contrast, the opportunist fungi (asperigillus sp., candida sp., cryptococcus sp., mucor sp., and rhizopus sp.) are widely distributed in nature, ...1977848497
transmammary passage of strongyloides sp. larvae in the human host.the prevalence of infection with strongyloides fuelleborni and hookworms (ancylostoma duodenale and necator americanus), and the possible transmammary passage of these parasites, was studied in the people of a village in bulapé, zaire, africa. stool examinations revealed that 34% of 76 infants under 200 days of age were infected with s. fuelleborni and 8% were infected with hookworms. infection rates in the general population were 44% for s. fuelleborni and 90% for hookworms. the examination of ...1977848643
wound infection. acute versus chronic cholecystitis.wound infection in 239 patients who underwent cholecystectomy were analyzed retrospectively. seventeen per cent of the patients with acute cholecystitis had wound infection compared with 8.9 per cent of patients with chronic cholecystitis. bacteriology of wound infections revealed staphylococcus aureus in 76.4 per cent of the chronic cholecystitis group and in 12.5 per cent of the acute cholecystitis group. wound infection in the acute cholecystitis group involved gram-negative rods predominantl ...1977848657
anaerobic bacteria in otitis media.anaerobic bacteria, peptostrepotococcus intermedius and propionibacterium acnes, were found in mixed culture specimens from four to ten tested cases of chronic secretory otitis media. these anaerobic bacteria were in a mixed infection flora with aerobic bacteria most often staphylococcus epidermidis and cornybacterium sp. which do not fit any established species. the findings of anaerobic bacteria in otitis media is consistent with the sporadic report of the involvement of anaerobic bacteria in ...1977848830
light and electron microscope study of thelohania solenopsae n. sp. (microsporida: protozoa) in the red imported fire ant, solenopsis invicta. 1977850074
"spring rise" in fecal nematode egg counts in sheep in maine.a "spring rise" in fecal nematode egg counts occurred in maine sheep housed under conventional conditions during 2 years. nematodes causing this increase were haemonchus contortus in one year and ostertagia sp in the other. the increase in egg counts was greater in sheep that lambed than in unbred sheep. most of the eggs in the spring rise were probably produced by worms that passed the winter in developmental arrest in the sheep. some of the female worms probably came from contaminated bedding ...1977851288
fosfomycin in chronic urinary infections.fosfomycin has been used on 40 patients with serious urinary infections of different clinical nature, and produced by repetitive infection (prostatisms, carcinomas, lithiasis, hydronephrosis, etc.) the following were isolated in the urocultures: e. coli (60%), klebsiella (15%), p. aeruginosa (15.0%), proteus sp. (15.0%), staphylococcus (5%), s. faecalis (2.5%) and paracolobactrum coliforme (2.5%). all the germs isolated before treatment were sensitive to fosfomycin. the results, as regards respo ...1977851501
determination of the absolute configuration at c-20 and c-24 of ergosterol in ascomycetes and basidiomycetes by proton magnetic resonance spectroscopy.samples of ergosterol isolated from saccharomyces cerevisiae, neurospora crassa, and agaricus sp., and commercial ergosterol all displayed identical proton magnetic resonance (pmr) spectra at 220 mhz. from the effects produced on the doublet for c-21 by epimerization at c-20 and c-24 in sterols of known configuration, the absolute configurations at these positions in ergosterol were determined. the data demonstrate that ergosterol from both ascomycetes and basidiomycetes is the same and that at ...1977853879
stephanofilaria okinawaensis n. sp. from cutaneous lesions on the teats of cows in japan.this is the first report on the occurrence of stephanofilarial sore on the teats of cows in the nansei (south western) islands, japan, and on the taxonomical position of etiological parasites. stephanofilaria worms were collected from cutaneous lesions of affected teats of cows in such proportion that the sex ratio of worms might be one male to three females. morphological and anatomical observation was made on parasites suspended in lactophenol and by the scanning electron microscope. as a resu ...1977854085
purification of human erythrocyte adenosine deaminase.in summary we have shown the following:1) human erythrocyte ada has been purified approximately 800,000 fold (sp. act. 538 micronmol/min/mg at 37 degrees) to apparent homogeneity using antibody affinity chromatography. 2) the enzyme was shown to be a single polypeptide chain with estimated molecular weight of approximately 38,000. 3) the 3 electrophoretic forms of erythrocyte ada purified simultaneously by this technique were indistinguishable by sds gel electrophoresis under reducing conditions ...1977855706
bacterial stalk rot of maize, its symptoms and host-range.stalk rot of maize, caused by erwinia carotovora f. sp. zeae sabet (re-designated as pectobacterium chrysanthemi pathovar. zeae by kelman 1974) showed first premature withering and drying up of the uppermost leaves which was soon followed by the lower leaves. the rot either extended from the base upwards (basal rot) or from the top downwards (top rot). in the case of basal rot, the leaves become yellow and the infected tissue becomes brown, soft, and water soaked. internally, the stalk turns int ...1977857508
studies on certain aspects of chemical control of bacterial stalk rot disease of maize.sandoz seed dressing 6335 showing high efficacy in checking the growth of the maize stalk rot pathogen erwinia carotovora f. sp. zeae sabet in culture. brestan, antracol, difolatan, aratan, duter, ceresan wet, flit-406, cuman, blitox-50, streptocycline, agrimycin, terramycin, actidione, aureomycin, chloromycetin, penicillin g, and streptomycin were moderately effective. the rest of the 35 chemicals was negligible in its influence. 15 different chemicals, namely agrimycin, streptocycline, chlorom ...1977857509
meningoencephalitis due to acanthamoeba sp. pathogenesis and clinico-pathological study.amebic meningoencephalitis (am) and primary amebic meningoencephalitis (pam) are infectious diseases essentially confined to the central nervous system (cns) and caused by free-living amebas of the genus acanthamoeba (a.) and naegleria (n.) respectively. am due to a. sp. (acanthamoeba castellanii and acanthamoeba culbertsoni) have been reported in chronically ill debilitated individuals, some of them under immunosuppressive therapy, or in immunologically impaired patients without a history of re ...1977857580
the reactivity of human erythrocyte membrane cholesterol with a cholesterol oxidase.cholesterol oxidase (ec 1.1.3.6, brevibacterium sp.), which catalyzes the reaction: cholesterol + o2 leads to delta4-cholestenone + h2o2, has no effect on the cholesterol of intact (human) erythrocytes and of "resealed" ghosts, when it is present only outside these ghosts. the cholesterol of "leaky" ghosts, of "resealed" ghosts with enzyme trapped within, and of "inside-out" vesicles, was completely oxidized. this pattern indicates that the inner ((cytoplasmic) membrane surface must be exposed ...1977857889
dactylogyrus atripinnei sp. n. from the blackfin sucker in tennessee.a new species of dactylogyrus, d. atripinnei, is described from the blackfin sucker, moxostoma atripinne bailey, collected from the barren river drainage in tennessee.1977859080
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