| in vitro embryo development and blastocyst hatching rates following vitrification of river buffalo embryos produced from oocytes recovered from slaughterhouse ovaries or live animals by ovum pick-up. | the present study was undertaken to determine whether the source of oocytes (ovum pick up versus slaughterhouse ovaries) affected in vitro embryo production and embryo survival (as measured by blastocyst hatching rates) following vitrification in buffaloes (bubalus bubalis). oocytes recovered from live buffaloes (n=6) by ovum pick up (opu) and by manual aspiration from slaughterhouse ovaries were in vitro matured, fertilized and cultured to blastocyst stage under same culture conditions. vitrifi ... | 2008 | 17689038 |
| duck egg yolk in extender improves the freezability of buffalo bull spermatozoa. | we investigated the use of duck egg yolk (dey), guinea fowl egg yolk (gfey) and indian indigenous hen (desi) egg yolk (idey) in extender for improving the post-thaw quality of buffalo (bubalus bubalis) bull spermatozoa, and compared it with commercial hen egg yolk (chey; control). for this purpose, two consecutive ejaculates of semen from each of two nili-ravi buffalo bulls were collected on 1 day each week for 5 weeks (replicates; n=5) with artificial vagina (42 degrees c). split pooled ejacula ... | 2008 | 17709214 |
| seasonal variation in nuclear dna integrity of frozen-thawed spermatozoa from thai ai swamp buffaloes (bubalus bubalis). | in this study, we investigated the susceptibility of frozen-thawed swamp buffalo sperm nuclear dna to undergo controlled acid-induced denaturation in situ, as analysed by flow cytometry, and aimed to correlate the results with sperm head morphology over three seasons in tropical thailand. artificial insemination (ai) doses (n = 218) from 18 ai buffalo sires, prepared between 1980 and 1989 and 2003 and 2005, were tested and compared among three seasons, the rainy season, july-october; winter, nov ... | 2007 | 17718812 |
| physicochemical properties and oxidative inactivation of soluble lectin from water buffalo (bubalus bubalis) brain. | lectins are carbohydrate-binding proteins present in a wide variety of plants and animals, which serve various important physiological functions. a soluble beta-galactoside binding lectin has been isolated and purified to homogeneity from buffalo brain using ammonium sulphate precipitation (40-70%) and gel permeation chromatography on sephadex g50-80 column. the molecular weight of buffalo brain lectin (bbl) as determined by sds-page under reducing and non-reducing conditions was 14.2 kda, howev ... | 2008 | 17763948 |
| molecular detection of leptospira species in aborted fetuses of water buffalo. | | 2007 | 17766812 |
| water buffalo: neglected, misunderstood. | | 1982 | 17788664 |
| pathogenic 'bison-type' mycobacterium avium subspecies paratuberculosis genotype characterized from riverine buffalo (bubalus bubalis) in north india. | despite low per-animal productivity of ruminants in developing countries, johne's disease has not been investigated in buffaloes, which are primarily found in these countries. this is due to lack of expertise, diagnostic kits and priority to production diseases like johne's disease. presence of pathogenic mycobacterium avium subspecies paratuberculosis (map) was investigated by screening of target tissues (mesenteric lymph nodes and large intestine) by culture and is 900 pcr, in 50 sacrificed bu ... | 2008 | 17804069 |
| an enzyme-linked immunosorbent assay (elisa) to measure growth hormone level in serum and milk of buffaloes (bubalus bubalis). | an indirect sandwich elisa to measure growth hormone level in serum and milk of buffaloes was developed. the assay was based on purified anti rbst igg raised in rabbits and chicken and rabbit anti chicken igg horseradish peroxidase. the assay was validated in terms of sensitivity, specificity, precision and recovery. parallelism was demonstrated between the standard curve and serially diluted serum, milk and pituitary derived growth hormone. sensitivity of the assay was 0.1 ng/ml. recovery of ex ... | 2007 | 17821853 |
| resolving a zoological mystery: the kouprey is a real species. | the kouprey is a rare and enigmatic forest ox discovered by scientists in cambodia only in 1937. numerous morphological hypotheses have been proposed for the origin of the kouprey: that it is a species closely related to banteng and gaur, two other wild oxen of southeast asia; a morphologically divergent species placed in a separate genus, named novibos; a wild species linked to aurochs and domestic cattle; a vicariant population of banteng; a feral cattle; or a hybrid of banteng with either zeb ... | 2007 | 17848372 |
| expression of cytochrome p450 aromatase transcripts in buffalo (bubalus bubalis)-ejaculated spermatozoa and its relationship with sperm motility. | the cytochrome p450 aromatase (aromp450) deficient mice are infertile due to an impairment of spermatogenesis associated with a decrease in sperm motility and inability to fertilize oocytes. the sperm analysis showed decreased sperm motility in humans, having cyp19 gene mutations. further, in human, it was hypothesized that aromatase could be used as marker of sperm quality, particularly in the acquisition of its motility. however, there is no information regarding the expression of aromp450 in ... | 2008 | 17851018 |
| molecular characterization of cdna encoding b. taurus cathelicidin-7 like antibiotic peptide from bone marrow cells of bubalus bubalis. | cathelicidins represent a diverse family of endogenous cationic antibiotic peptide present in all mammalian species. in the present study, a novel cathelicidin cdna was identified and characterized from bone marrow cells of buffalo (bubalus bubalis) using rt-pcr based approach. the cdna encodes a propeptide of 1.18 kda with net positive charge at neutral ph. the precursor peptide possesses a signal peptide of 29 amino acids and a biologically active peptide of 34 residues. comparison of sequence ... | 2008 | 17852341 |
| phylogeography and domestication of indian river buffalo. | the water buffalo- bubalus bubalis holds tremendous potential in livestock sector in many asian countries, particularly india. the origin, domestication and genetic structure of the indian river buffalo are poorly understood. therefore, to understand the relationship among the maternal lineages of indian river buffalo breeds and their domestication process, we analysed mitochondrial d-loop region of 217 animals representing eight breeds from eight different locations in india along with publishe ... | 2007 | 17915036 |
| biological and genetic analysis of a bovine-like coronavirus isolated from water buffalo (bubalus bubalis) calves. | we describe the isolation, biological and genetic characterization of a host-range variant of bovine coronavirus (bcov) detected in water buffalo (bubalus bubalis). by conventional and real-time rt-pcr assays, the virus was demonstrated in the intestinal contents of two 20-day-old buffalo calves dead of a severe form of enteritis and in the feces of additional 17 buffalo calves with diarrhea. virus isolation, hemagglutination and receptor-destroying enzyme activity showed that the buffalo corona ... | 2008 | 17916374 |
| cloning the swamp buffalo sry gene for embryo sexing with multiplex-nested pcr. | the polymerase chain reaction (pcr) is an efficient method for sexing embryos. the objective of this study was to develop an accurate and reliable method for sexing swamp buffalo (bubalus bubalis) embryos. the sry gene from swamp buffalo genomic dna was amplified by pcr, using primers based on the sequence of the holstein sry gene. this fragment was sequenced based on a blast search; the sry gene was highly conserved. using a southern blot, there was a strong signal in genomic dna only from male ... | 2007 | 17928043 |
| detection of bovine viral diarrhoea virus (bvdv) nucleic acid and antigen in different organs of water buffaloes (bubalus bubalis). | bovine viral diarrhoea virus (bvdv) is a pestivirus that infects mainly bovine cattle. nevertheless, there are several reports about infections in other members of the artiodactyla order including serological studies, that indicate infection of bvdv in buffaloes. the aim of this article is to study the presence of bvdv in three young water buffaloes, displaying nonspecific clinical signs, compatible with the bvdv infection. both immunohistochemistry and rt-pcr confirmed the presence of bvdv in t ... | 2008 | 17949764 |
| occurrence of antibodies against neospora caninum in water buffaloes (bubalus bubalis) on four ranches in corrientes province, argentina. | the aim of the present work was to describe the occurrence of antibodies to neospora caninum in water buffaloes on four ranches located in corrientes province in the northeast of argentina. antibodies against n. caninum were determined in sera of 449 water buffaloes by using an indirect fluorescent antibody test (ifat). a bayesian logistic regression mixed model was used to quantify the strength of association between positive serological results to n. caninum and gender, age and category (calf, ... | 2007 | 17950535 |
| follicular fluid concentrations of glucose, lactate and pyruvate in buffalo and sheep, and their effects on cultured oocytes, granulosa and cumulus cells. | the objective was to determine ovarian follicular fluid concentrations of glucose, lactate, and pyruvate in relation to follicle size in buffalo and sheep. the effect of varying concentrations of these substances on in vitro oocyte maturation, oocyte protein content, and granulosa and cumulus cell growth was also investigated. follicular fluid was aspirated from various sizes of follicles (from ovaries without a dominant follicle) collected from adult, cycling nonpregnant buffalo (bubalus bubali ... | 2008 | 17950822 |
| molecular identification of cryptosporidium parvum and giardia duodenalis in the italian water buffalo (bubalus bubalis). | livestock are commonly infected with protozoan parasites of the genera cryptosporidium and giardia, and some of the species and genotypes found in these animals have zoonotic significance. we characterized isolates of both parasites recovered from the italian water buffalo (bubalus bubalis), an economically important species whose milk is used for the production of "buffalo mozzarella" fresh cheese. molecular analysis of the cryptosporidium small subunit ribosomal dna gene and of the giardia bet ... | 2007 | 17951009 |
| expression of the serotonin transporter (sert) in the choroid plexuses from buffalo brain. | choroid plexuses (cps) play pivotal roles in a wide range of processes that establish, survey, and maintain the biochemical and cellular status of the central nervous system. mammalian cps contain a very high density of serotonin receptors, and serotonin has been shown to affect cp functions. the serotonin transporter (sert) regulates the entire serotonergic system, including serotonin receptors by means of modulation of serotonin concentration in the extracellular fluid. in this study, the expr ... | 2007 | 17957753 |
| genetic diversity and differentiation of chinese domestic buffalo based on 30 microsatellite markers. | to determine genetic diversity and evolutionary relationships among chinese buffalo populations, 18 indigenous swamp buffalo populations and two introduced river buffalo breeds were genotyped for 30 microsatellite loci. the mean number of alleles across the 30 loci was 8.13, and the expected heterozygosity ranged from 0.517 (yanjin) to 0.609 (dehong). although there was abundant genetic variation, genetic differentiation between chinese buffalo populations was low, with only 2.8% of the total ge ... | 2007 | 17980000 |
| application of recombinant sjc26gst for serodiagnosis of schistosoma japonicum infection in water buffalo (bos buffelus). | schistosomiasis japonica is currently the most serious parasitic disease in mainland china and it is estimated that several million people are infected. furthermore, it is also responsible for the deaths of many domestic animals. in order to establish an effective diagnostic method, the gene encoding sjc26gst was cloned and expressed in escherichia coli as a fusion protein with his-tag. the purified resjc26gst was used as an antigen for an enzyme-linked immunosorbent assay (elisa) and for immuno ... | 2007 | 17997225 |
| the sarcocystis-cyst containing beef and pork as the sources of natural intestinal sarcocystosis in thai people. | human intestinal sarcocystosis is a zoonotic disease caused by two coccidians, i.e. sarcocystis fusiformis (syn. s. bovihominis, s. hominis) due to consumption of raw infected beef and sarcocystis meischeriana (syn. s. suihominis) due to consumption of infected raw pork. in 1987, survey of the macroscopic s. fusiformis cysts in market beef mainly from old water buffalos aged more than 15 years were commonly observed in bangkok. in 2005, the macroscopic cyst was no longer seen in beef of cattle a ... | 2007 | 18041433 |
| effect of antibiotics in extender on bacterial and spermatozoal quality of cooled buffalo (bubalus bubalis) bull semen. | the present study was designed to study the effect of traditional antibiotic combination (streptomycin and penicillin; sp) and relatively modern combination of antibiotics (gentamycin, tylosin, lincomycin and spectinomycin; gtls) in extender on bacterial control and spermatozoal quality of liquid buffalo bull semen stored at 5 degrees c. semen collected from nili-ravi buffalo bulls (n = 10) was diluted with skim milk extender containing either sp (streptomycin 1000 microg/ml and penicillin 1000 ... | 2008 | 18042206 |
| characterization of smoc-1 uncovers two transcript variants showing differential tissue and age specific expression in bubalus bubalis. | secreted modular calcium binding protein-1 (smoc-1) belongs to the bm-40 family which has been implicated with tissue remodeling, angiogenesis and bone mineralization. besides its anticipated role in embryogenesis, smoc-1 has been characterized only in a few mammalian species. we made use of the consensus sequence (5' cacctctccacctgcc 3') of 33.15 repeat loci to explore the buffalo transcriptome and uncovered the smoc-1 transcript tagged with this repeat. the main objective of this study was to ... | 2007 | 18042303 |
| association of microsatellite (gt)n polymorphism at 3'utr of nramp1 with the macrophage function following challenge with brucella lps in buffalo (bubalus bubalis). | brucella abortus is a facultative intracellular pathogen that survives and replicates in host macrophages. hence, macrophage function plays an important role in influencing natural resistance/susceptibility to intracellular pathogen. the natural resistance associated macrophage protein 1 (nramp1; erstwhile referred as ity/lsh/bcg), a transmembrane protein, regulates activity of macrophages against intracellular pathogens. in bovine, natural resistance to brucellosis is significantly associated w ... | 2008 | 18078724 |
| a radiation hybrid map of river buffalo (bubalus bubalis) chromosome 1 (bbu1). | the largest chromosome in the river buffalo karyotype, bbu1, is a submetacentric chromosome with reported homology between bbu1q and bovine chromosome 1 and between bbu1p and bta27. we present the first radiation hybrid map of this chromosome containing 69 cattle derived markers including 48 coding genes, 17 microsatellites and four ests distributed in two linkage groups spanning a total length of 1330.1 cr(5000). the rh map was constructed based on analysis of a recently developed river buffalo ... | 2007 | 18160788 |
| effect of selenium and copper supplementation on blood metabolic profile in male buffalo (bubalus bubalis) calves. | twenty male buffalo calves (8-9 months, 112.1 +/- 7.69 kg) were divided into four groups of five animals in each and fed diets without (t1) or supplemented with 0.3 ppm selenium (se) (t2), 0.3 ppm se + 10 ppm copper (cu) (t3), and 10 ppm cu (t4) for 120 days during which blood samples were collected on day 0, 40, 80, and 120. concentrations of glucose, total protein, urea, uric acid, and creatinine were similar in all the four groups, but the level of globulin was significantly (p < 0.01) increa ... | 2008 | 18185997 |
| multi-host transmission dynamics of schistosoma japonicum in samar province, the philippines. | among the 6.7 million people living in areas of the philippines where infection with schistosoma japonicum is considered endemic, even within small geographical areas levels of infection vary considerably. in general, the ecological drivers of this variability are not well described. unlike other schistosomes, s. japonicum is known to infect several mammalian hosts. however, the relative contribution of different hosts to the transmission cycle is not well understood. here, we characterize the t ... | 2008 | 18215106 |
| a novel method for species identification in milk and milk-based products. | this study describes a method for species-specific detection of animal dna from different species (cattle, sheep, goat, water buffalo) in milk and dairy products. a primer set was designed in conserved region on the basis of the alignment of the sequence codifying the genomic kappa-casein gene in order to amplify all four species with a single primer pair. polymorphisms were detected via minisequencing with extension primers designed in conserved sequences for haplotype determination that allow ... | 2008 | 18226307 |
| a survey of abortifacient infectious agents in livestock in luzon, the philippines, with emphasis on the situation in a cattle herd with abortion problems. | in the philippines, insufficient consideration has been given to the implementation of systematic control measures against major abortifacient infectious agents in livestock. to elucidate the epidemiology of abortifacient infectious agents in livestock, the prevalence of four abortifacient agents was assessed. initially, a total of 96 cattle including 17 cows with history of abortion were examined in a herd in luzon at the request of the farm owner. six (35.3%) of the 17 aborting cows were found ... | 2008 | 18243149 |
| [molecular phylogeny of the gayal inferred from the analysis of cytochrome b gene entire sequences]. | the gayal (bos frontalis) is a very rare, semi-wild and semi-domestic bovine species. there still exist remarkable divergences on the gayal's origin and phylogenetic status. the cytochrome b (cyt b) gene entire sequences (1,140 bp) of 11 gayals were sequenced and analyzed. combined with other bovine cyt b entire sequences cited in genbank, the phylogenetic trees of genus bos were reconstructed by neighbor-joining (nj) and maximum parsimony (mp) methods with bubalus bubalis as outgroup. sequence ... | 2008 | 18244904 |
| a radiation hybrid map of river buffalo (bubalus bubalis) chromosome 7 and comparative mapping to the cattle and human genomes. | a preliminary radiation hybrid (rh) map containing 50 loci on chromosome 7 of the domestic river buffalo bubalus bubalis (bbu; 2n = 50) was constructed based on a comparative mapping approach. the rh map of bbu7 includes thirty-seven gene markers and thirteen microsatellites. all loci have been previously assigned to bos taurus (bta) chromosome bta6, which is known for its association with several economically important milk production traits in cattle. the map consists of two linkage groups spa ... | 2008 | 18253035 |
| comparative fish-mapping of twelve loci in river buffalo and sheep chromosomes: comparison with hsa8p and hsa4q. | twelve loci (11 of type i and 1 of type ii) previously fish-mapped in cattle were comparatively fish-mapped in both river buffalo chromosome 1p (bbu1p) and homologous chromosome 26 of sheep (oar26), extending the cytogenetic maps in both chromosome species and providing a more precise localization of these loci in single chromosome bands than previous locations on bta27. bovine bac clones containing dctd, c4orf20, casp3, tlr3, msr1, fat, lonrf1, dlc1, c8orf41, cssm036, lsm1 and eif4ebp1 were use ... | 2008 | 18253036 |
| cysteamine supplementation of in vitro maturation medium, in vitro culture medium or both media promotes in vitro development of buffalo (bubalus bubalis) embryos. | the effects of supplementation of in vitro maturation (ivm) or in vitro culture (ivc) or both ivm and ivc media with cysteamine on the yield, hatching rate (hr) and total cell number (tcn) of buffalo blastocysts were examined. oocytes obtained from slaughterhouse buffalo ovaries were subjected to ivm and ivf. the ivm or ivc media were supplemented with 0, 50, 100 or 200 microm cysteamine. supplementation of ivm medium with 50 microm cysteamine increased (p < 0.01) the cleavage rate and blastocys ... | 2008 | 18255014 |
| comparative therapeutic effect of toltrazuril, sulphadimidine and amprolium on eimeria bovis and eimeria zuernii given at different times following infection in buffalo calves (bubalus bubalis). | we compared the therapeutic effect of three anticoccidial drugs (toltrazuril, sulphadimidine and amprolium) in buffalo (bubalus bubalis) calves experimentally infected with eimeria bovis (e. bovis) and e. zuernii oocysts (3 x 104oocyst/calf). buffalo calves (1.5-4 month old, 70-kg body weight) were randomly allocated into 3 groups (9 calves each). group t was experimentally infected with oocysts and treated with toltrazuril (20 mg/kg bw twice orally at a 1-week interval). group s was experimenta ... | 2008 | 18262668 |
| development of a pronuclear dna microinjection technique for production of green fluorescent protein-expressing bubaline (bubalus bubalis) embryos. | oocytes from abattoir-derived bubaline (bubalus bubalis) ovaries were subjected to ivm and ivf; the objective was to develop a pronuclear dna microinjection technique to produce embryos expressing green fluorescent protein (gfp). the largest proportion (61.2%) of zygotes in which one (1 pn) or two pronuclei (2 pn) were visible was when centrifugation (14,000 x g for 15 min) was done 16 h after insemination. centrifugation had no adverse effects on cleavage rate, development to morulae/blastocyst ... | 2008 | 18272213 |
| oocyte recovery by ovum pick up and embryo production in river buffaloes (bubalus bubalis). | ovum pick up (opu) was conducted twice a week for 12 weeks in six cycling, non-descriptive (local breed), indian buffaloes to study the efficiency of opu on recovery of oocytes for embryo production. opu was performed using an ultrasound equipment with a 5-mhz transvaginal transducer, a single-lumen, 18-gauge, 55-cm-long needle and a constant vacuum pressure of 110 mmhg. the number and size of follicles were determined before puncture. the recovered oocytes were graded, washed, matured for 24 h ... | 2008 | 18282214 |
| post-exposure serological and bacteriological responses of water buffalo (bubalus bubalis) to brucella abortus biovar 1 following vaccination with brucella abortus strain rb51. | serological and bacteriological responses to brucella abortus biovar 1 following vaccination with b. abortus strain rb51 (rb51) were evaluated in thirty domestic water buffalo (bubalus bubalis) randomly divided into five treatment groups. groups i to v received, respectively, the recommended dose (rd) of rb51 vaccine once, rd twice 4 weeks apart, double rd once, double rd twice 4 weeks apart, and saline once (control). vaccination did not result in a serological response. experimental animals re ... | 2007 | 18293615 |
| first radiation hybrid map of the river buffalo x chromosome (bbux) and comparison with btax. | we report the first radiation hybrid map of the river buffalo x chromosome generated from a recently constructed river buffalo (bubalus bubalis) whole-genome radiation hybrid panel (bburh(5000)). this map contains a total of 33 cattle-derived markers, including 10 genes, four ests and 19 microsatellites. the markers are distributed in two linkage groups: lg1 contains eight markers spanning 125.6 cr, and lg2 contains 25 markers spanning 366.3 cr. lg1 contains six markers in common with bovine seq ... | 2008 | 18307583 |
| isolation of new pregnancy-associated glycoproteins from water buffalo (bubalus bubalis) placenta by vicia villosa affinity chromatography. | the present study describes the isolation and characterization of new pregnancy-associated glycoprotein molecules (pag) from midpregnancy and late-pregnancy placentas in the water buffalo (bubalus bubalis). after extraction, the homogenates are subjected to acid and ammonium sulfate precipitations followed by deae chromatography. subsequently, the water buffalo pag (wbpag) from these solutions are enriched by vicia villosa agarose (vva) affinity chromatography. as determined by western blotting ... | 2008 | 18308351 |
| diagnostic and prognostic indicators of omasal impaction in buffaloes (bubalus bubalis). | twelve of 46 female buffaloes with abdominal disorders were diagnosed with omasal impaction. they had been fed finely chopped machine-prepared straw. they were characterised by anorexia, an absence of defecation, abdominal distension, ruminal hypomotility or atony and a suspension of rumination. omasal impaction was confirmed upon left flank laparorumenotomy on the basis of the size of the omasum and the consistency of its contents. after ruminal evacuation, a long flexible pipe was introduced t ... | 2008 | 18310560 |
| molecular characterization of toll-like receptor 2 of ruminants and comparative expression in nilgai (boselaphus tragocamelus) vs. buffalo (bubalus bubalis). | toll-like receptors play role in the innate immune responses and orchestrate the adaptive immunity by induction of proinflammatory cytokines and up-regulation of costimulatory molecules. the present study has characterized tlr2 cdna in nilgai, buffalo, sheep and goat that recognizes the peptidoglycan of gram-positive bacteria. tlr2 coding sequences were amplified from monocytes cdna and cloned in pgemt-easy vector for nucleotide sequencing. sequence analysis revealed 2355-bp-long tlr2 open readi ... | 2008 | 18312593 |
| cytosolic and microsomal glutathione-s-transferases from bovine filarial worms setaria cervi. | the work presented here deals with the status of glutathione-s-transferase (gst; e.c. 2.5.1.18), the major enzyme of the phase ii detoxification pathway, in bovine filarial worms setaria cervi. gst activity was determined in various subcellular fractions of bovine filarial worms s. cervi (bubalus bubalis linn.) and was found to be mainly associated with cytosolic and microsomal fractions. the respective specific activities of the enzyme from cytosolic and microsomal fractions of s. cervi females ... | 2007 | 18314670 |
| mannose-binding lectin haplotypes influence brucella abortus infection in the water buffalo (bubalus bubalis). | a case-control study established that the haplotype pair hya/hya at the mbl (mannose binding lectin) locus of water buffalo is associated with resistance to brucella abortus infection (p < 10(-7)) and the haplotype pairs lyd/lyd with susceptibility to the same pathogen (p < 10(-7)). the subjects included in the present study were tested twice-at a 1-month interval-for the presence of anti-b. abortus antibodies in the serum by agglutination, complement fixation and flow cytometry. cases (335 subj ... | 2008 | 18330558 |
| in vitro embryo production in buffalo (bubalus bubalis) using sexed sperm and oocytes from ovum pick up. | the objective was to explore the use of sexed sperm and opu-derived oocytes in an ivp system to produce sex-preselected bubaline embryos. oocytes were recovered from 20 fertile murrah and nili-ravi buffalo cows by repeated (twice weekly) ultrasound-guided transvaginal ovum pick up (opu), or by aspiration of abbatoir-derived bubaline ovaries, and subjected to ivf, using frozen-thawed sexed or unsexed bubaline semen. on average, 4.6 oocytes were retrieved per buffalo per session (70.9% were grades ... | 2008 | 18336893 |
| genetic relationship and diversity analysis of indian water buffalo (bubalus bubalis). | the water buffalo (bubalus bubalis) is an important dairy animal on the indian subcontinent and in southeast asian countries. the diversity and differentiation among 12 populations or breeds of buffalo were studied. data were generated and analyzed from 527 animals belonging to 10 recognized breeds and 2 additional populations of indian buffalo by using 22 microsatellite loci. relationships among buffalo breeds and populations were estimated based on genetic distances. the bayesian analysis grou ... | 2008 | 18344309 |
| water buffalo mozzarella cheese stored in polysaccharide-based gels: correlation between prolongation of the shelf-life and physicochemical parameters. | an innovative packaging system has been developed, based on natural gels, that has shown the peculiar characteristic to strongly increase the shelf life of water buffalo mozzarella cheese. to explain the mechanism of action of the gel, measurements of ca and na in the cheese and in the storage liquid were carried out, together with ph determination. a correlation has been found between the constant level of ca and ph in the cheese and the prolongation of nutritional characteristics; in fact, bot ... | 2008 | 18349224 |
| capacitation-associated protein tyrosine phosphorylation starts early in buffalo (bubalus bubalis) spermatozoa as compared to cattle. | a comparative study was conducted on protein tyrosine phosphorylation events of capacitating sperm of two ruminant species, cattle and buffalo. ejaculated cattle and buffalo bull spermatozoa were suspended separately in sp-talp medium at 50 x 10(6)ml(-1) and incubated at 38.5 degrees c with 5% co(2) in air in the absence or presence of heparin for a period of 6h. the extent of sperm capacitation after various periods of incubations was assessed by lysophosphatidyl choline-induced acrosome reacti ... | 2009 | 18358647 |
| organization and differential expression of the gaca/gata tagged somatic and spermatozoal transcriptomes in buffalo bubalus bubalis. | simple sequence repeats (ssrs) of gaca/gata have been implicated with differentiation of sex-chromosomes and speciation. however, the organization of these repeats within genomes and transcriptomes, even in the best characterized organisms including human, remains unclear. the main objective of this study was to explore the buffalo transcriptome for its association with gaca/gata repeats, and study the structural organization and differential expression of the gaca/gata repeat tagged transcripts ... | 2008 | 18366692 |
| epizootic abortion related to infections by chlamydophila abortus and chlamydophila pecorum in water buffalo (bubalus bubalis). | water buffalo (bubalus bubalis) are affected by high rates of embryonic mortality and abortion related to infectious diseases and non-infectious factors. a number of viral and bacterial infections have been associated with reproductive failure, but there is limited information on the role of chlamydial infections. in order to investigate the presence and the role of chlamydiaceae in water buffalo a retrospective study was performed in a herd with a history of reproductive failure. during an 11-m ... | 2008 | 18374406 |
| peptidomic approach based on combined capillary isoelectric focusing and mass spectrometry for the characterization of the plasmin primary products from bovine and water buffalo beta-casein. | the main peptides produced by hydrolysis of water buffalo beta-casein with plasmin were characterized by capillary electrophoresis and mass spectrometry and compared with their bovine homologous. a novel breakdown product arising from the hydrolysis of water buffalo beta-casein, originated by the presence of a plasmin-sensitive lys bond at position 68 was identified, which was not present in bovine beta-casein. on the basis of this evidence, an improved procedure for the detection and the differ ... | 2008 | 18400224 |
| acute coliform mastitis in buffaloes (bubalus bubalis): clinical findings and treatment outcomes. | this report was delineated to study the clinical, bacteriological and therapeutic aspects concerned with acute coliform mastitis in buffaloes. bacteriological examination of 80 quarter milk samples obtained aseptically from 56 buffaloes with acute mastitis revealed that coliform bacteria was the most common pathogen (45 cases) followed by staphylococcus aureus (seven cases) then streptococcus uberis (three cases), and streptococcus agalactiae (one case). clinically, hotness, swelling and painful ... | 2008 | 18422251 |
| ameliorative effects of boron on serum profile in buffalo (bubalus bubalis) fed high fluoride ration. | an experiment was undertaken to evaluate the protective role of boron on the serum profile of buffalo calves fed a high fluoride ration. twelve male murrah buffalo (bubalus bubalis) calves of 6-8 months age, divided into three groups of four calves in each, were fed basal diets and supplemented with sodium fluoride (naf, 60 ppm) alone or in combination with borax (na2b4o7.10h2o, 140 ppm) for 90 days. boron (b) was added in the ration as borax to make @140 ppm boron (elemental b) on dm basis in t ... | 2008 | 18422253 |
| effect of breeding season on in vivo oocyte recovery and embryo production in non-descriptive indian river buffaloes (bubalus bubalis). | the present study was carried out to examine the effect of season on in vivo oocyte recovery and embryo production in non-descriptive, indian river buffaloes (bubalus bubalis). ovum pick up (opu) was conducted twice a week for 8 weeks during peak (october-march) and low (april-september) breeding season in live buffaloes (n=6). opu was performed using ultrasound equipment with a 5mhz transvaginal transducer, a single lumen 18-gauge, 55-cm long needle and a constant vacuum pressure of 110mmhg. th ... | 2009 | 18440168 |
| effect of type of cryoprotectant on morphology and developmental competence of in vitro-matured buffalo (bubalus bubalis) oocytes subjected to slow freezing or vitrification. | the present study examined the effects of different cryoprotectants on morphology and developmental competence of in vitro-matured buffalo oocytes after slow freezing or vitrification. after slow freezing in dimethyl sulfoxide (dmso), ethylene glycol (eg) or 1,2-propanediol (proh), at 1.0 or 1.5 m each, the proportion of morphologically normal oocytes recovered was significantly higher (p < 0.05) with 1.5 than 1.0 m for all cryoprotectants and was highest (p < 0.05) for 1.5 m dmso. following vit ... | 2008 | 18462611 |
| sex chromosome abnormalities and sterility in river buffalo. | thirteen male river buffaloes, 119 females with reproductive problems (which had reached reproductive age but had failed to become pregnant in the presence of bulls) and two male co-twins underwent both clinical and cytogenetic investigation. clinical analyses performed by veterinary practitioners revealed normal body conformation and external genitalia for most females. however, some subjects showed some slight male traits such as large base horn circumference, prominent withers and tight pelvi ... | 2008 | 18467836 |
| chromosomal expression and localization of aphidicolin-induced fragile sites in the standard karyotype of river buffalo (bubalus bubalis). | the present study reports on the chromosomal expression and localization of aphidicolin-induced fragile sites in the standard karyotype of river buffalo (bubalus bubalis, 2n = 50) with the aim of establishing a 'fragile site map' of the species. totally, 400 aphidicolin-induced breakages were analyzed from eight young and clinically healthy animals, four males and four females; these breakages were localized in 106 rbg-negative chromosome bands or at the band-interband regions. the number of bre ... | 2008 | 18467845 |
| sequential pathological studies in asian water buffaloes infected intratracheally with absidia corymbifera. | zygomycosis was produced experimentally in asian water buffalo calves (bubalus bubalis) by intratracheal inoculation of sporangiospores of absidia corymbifera. infected animals exhibited dullness, depression, partial anorexia, initial pyrexia, mucopurulent nasal and ocular discharge and coughing during the first week. there was no mortality at any stage of the experiment, which continued for 30 days. the gross and microscopic lesions were restricted to the lungs and there was no dissemination of ... | 1998 | 18473535 |
| to catch a buffalo: field immobilisation of asian swamp buffalo using etorphine and xylazine. | to demonstrate the efficacy of a mixture of etorphine and xylazine to safely immobilise wild buffalo (bubalus bubalis) in the field. | 2008 | 18498561 |
| restricted genetic diversity in the ubiquitous cattle parasite, sarcocystis cruzi. | although parasites of the genus sarcocystis have likely cycled between bovine herbivores and canine carnivores for tens of millions of years, humans may have profoundly influenced the ecology and evolution of those prevalent in domesticated dogs and cattle. to preliminarily assess the possibility of such anthropogenic effects, we surveyed genetic variation in conserved (18s small subunit) and variable (its-1) portions of ribosomal dna from a large sample of sarcocystis cruzi occurring in taurine ... | 2008 | 18501682 |
| echinococcus ortleppi and e. granulosus g1, g2 and g3 genotypes in italian bovines. | to increase the knowledge on echinococcus genotypes infesting cattle and water buffaloes (bubalus bubalis) born and bred in italy, the germinal layer of hydatid cysts was collected from the liver and the lungs of 80 animals slaughtered in 2007. two mitochondrial genes (the cytochrome c oxidase subunit i and the nadh subunit i) were tested by pcr. four genotypes were identified: g1 (sheep strain), g2 (tasmanian sheep strain), g3 (buffalo strain), and g5 (cattle strain). fertile cysts were detecte ... | 2008 | 18514422 |
| dna-based vaccines protect against zoonotic schistosomiasis in water buffalo. | schistosomiasis japonica is an endemic, zoonotic disease of major public health importance in china where water buffaloes account for approximately 75% of disease transmission. interventions that reduce schistosome infection in water buffaloes will enhance their health simultaneously reducing disease transmission to humans. while chemotherapy has proved successful, it requires continued time consuming and expensive mass treatments. a more sustainable option would be development of vaccines that ... | 2008 | 18524429 |
| assessment of water buffalo health and productivity in a communal management system in the philippines. | this study aimed to generate a profile of the health and productivity of water buffaloes in a communal setting. using the epi-info version 6.04 for data management, a coded information system was used to accommodate data coming from the reference population. calves and cows that were born and milked, respectively, were enrolled and monitored for six months. the key outcomes of interest monitored in this study included mortality, morbidity and productivity. results of the study showed a 93.7 perc ... | 2008 | 18551780 |
| molecular cloning and characterization of lingual antimicrobial peptide cdna of bubalus bubalis. | antimicrobial peptides form a crucial component of innate immune system, making it a highly effective first line of defense in animals. in the study, lingual antimicrobial peptide cdna of bubalus bubalis has been characterized. the characterized cdna has complete orf of 195 bases. the signal sequence of buffalo lap comprised of n-terminal 1-20 amino acids and mature peptide from 23-64 amino acids. the percentage of similarity of buffalo lap and buffalo ebd at nucleotide and amino acid level was ... | 2009 | 18558415 |
| relationship between peripheral plasma inhibin and fsh concentrations in sahiwal cows (bos indicus) and murrah buffaloes (bubalus bubalis) during estrous cycle. | the present investigation was undertaken to study the relationship between circulating inhibin and fsh concentrations during the estrous cycle in buffaloes and sahiwal cattle. the pattern of inhibin concentrations was similar, with peak concentrations on day -2 (day 0 = day of estrus) and minimum concentrations on days 12 and 11 in buffaloes and cattle, respectively. circulating fsh concentrations were the highest on day 0 and lowest on days 8 and 13 in buffaloes and cattle, respectively. periph ... | 2008 | 18575966 |
| cryptosporidiosis in buffalo calves (bubalus bubalis): prevalence and potential risk factors. | the objective of the present study was to describe the prevalence and risk factors associated with cryptosporidiosis in buffalo calves in middle egypt. during one year, 458 fecal samples were collected from buffalo calves less than 3 month age in 55 small scale herds and examined for the presence of cryptosporidium oocysts. data describing age, gender, season, and herd management practices were gathered to assess potential risk factors. fecal examination showed that 14.19% of the examined calves ... | 2008 | 18575969 |
| fatal intestinal coccidiosis in a three week old buffalo calf (bubalus bubalus). | the water buffalo (bubalus bubalus) is important to the economy of several countries, especially in asia and brazil. little is known of the impact of coccidiosis in buffaloes. cattle and buffaloes are considered to have common species of eimeria but critical cross transmissions have not been made because it is difficult to raise these hosts coccidian free. clinical coccidiosis was confirmed post mortem in a 22-day old buffalo calf that died after a 3-4 day illness. oocysts morphologically identi ... | 2008 | 18576888 |
| evaluation of a fluorescence polarization assay for the detection of serum antibodies to brucella abortus in water buffalo (bubalus bubalis). | the fluorescence polarization assay (fpa) was evaluated for the serological diagnosis of brucellosis in water buffalo (bubalus bubalis) in southern italy. this assay uses o-polysaccharide prepared from brucella abortus lipopolysaccharide conjugated with fluorescein isothiocyanate as a tracer. it has many methodological advantages over older, more established tests and can be performed in a fraction of the time. sera from 890 buffalos from the campania region - 526 positive sera and 364 negative ... | 2008 | 18599128 |
| experimental transmission of foot-and-mouth disease among indian buffalo (bubalus bubalis) and from buffalo to cattle. | indian buffalo and cattle were infected experimentally with a serotype o strain of foot-and-mouth disease virus of buffalo origin. whereas intradermolingual inoculation of buffalo produced largely sub-clinical infection, inoculation in the dental pad produced vesicles in the mouth and on the feet. a buffalo infected via the dental pad transmitted infection to cattle and buffalo by direct contact with them for 24h. the contact-exposed buffalo developed (1) delayed-onset clinical signs, and (2) sh ... | 2008 | 18619607 |
| expression profile of toll like receptors in a range of water buffalo tissues (bubalus bubalis). | the present study was carried out to determine the expression profile of toll-like receptors (tlrs) 1-10 in buffalo peripheral blood mononuclear cells (pbmnc), neutrophils, spleen, liver, lung, heart, kidney, ovary and uterus using reverse transcriptase polymerase chain reaction (rt-pcr) with bovine tlr-specific primers the buffalo tlr partial nucleotide sequences had 95-98% nucleotide homology with bovine tlr sequences available in the genbank. pbmnc expressed all tlrs except tlr1 and neutrophi ... | 2008 | 18619678 |
| characterization of tlr4 signaling in water buffalo (bubalus bubalis). | lipopolysaccharide (lps) treatment of peripheral blood mononuclear cells (pbmc) isolated from the water buffalo resulted in the activation of tlr signaling intermediates as supported by the western blot of perk. activation of erk resulted in phosphorylation of ikappab-alpha which lead to its degradation which in turn followed by nuclear translocation of nf-kappab, which is also supported by the western blot analysis. the nuclear translocation of nf-kappab culminated in the induction of mrna expr ... | 2009 | 18648999 |
| structure-function studies of bubalus bubalis lingual antimicrobial peptide analogs. | antimicrobial peptides expressed on different epithelial lining are major components of the innate immune system. based on the deduced amino acid sequence of bubalus bubalis lingual antimicrobial peptide (lap) cdna (accession no. dq458768), five overlapping peptides lap(23-55), lap(42-64), lap(21-64), lap(1-26) and lap(1-64) were synthesized using solid phase fluorenylmethoxycarbonyl (fmoc) chemistry. circular dichroism spectroscopy of synthesized peptides revealed predominantly beta-structure f ... | 2009 | 18651233 |
| eimeria spp. in brazilian water buffalo. | eimeria species are frequently found in water buffalo (bubalus bubalis) in brazil. here, we report those eimeria spp. that infect buffalos during their first year of life. fresh fecal samples were examined from 2 groups (1 group/yr for 2 yr, 2000-2002), each with 18 water buffalo calves (both sexes), from birth through 12 mo of age, in selvíria, ms, brazil. five oocyst morphotypes were observed, i.e., eimeria ellipsoidalis and eimeria zuernii, both previously described from water buffalo, and 3 ... | 2009 | 18662041 |
| a proteomic characterization of water buffalo milk fractions describing ptm of major species and the identification of minor components involved in nutrient delivery and defense against pathogens. | water buffalo has been studied in relation to the exclusive use of its milk for the manufacture of high-quality dairy products. buffalo milk presents physicochemical features different from that of other ruminant species, such as a higher content of fatty acids and proteins. we report here a detailed proteomic analysis of buffalo skim milk, whey and milk fat globule membrane fractions. notwithstanding the poor information available on buffalo genome, identification of protein isoforms correspond ... | 2008 | 18668696 |
| prognostic value of various spermatological attributes as predictors of zona binding and zona penetration of buffalo (bubalus bubalis) semen. | twenty-four ejaculates from six (four ejaculates each) surti buffalo bulls aged 4-8 years were used to assess various attributes of spermatozoa influencing the zona-binding and zona-penetration tests. ejaculates from each bulls were subjected to in vitro sperm--zona binding and sperm--zona penetration tests (four replicates per bull) using immature buffalo oocytes. the average number of spermatozoa bound per oocyte was 27.79 +/- 5.90. the average number of spermatozoa penetrated per oocyte was 3 ... | 2009 | 18673329 |
| influence of heat stress on the cortisol and oxidant-antioxidants balance during oestrous phase in buffalo-cows (bubalus bubalis): thermo-protective role of antioxidant treatment. | in the present study, the effect of heat stress, which is commonly observed in the animals of upper egypt area in summer, as well as the effect of antioxidant treatment as a thermo-protective was examined. in this study, the animals (n = 120) were divided into winter group (n = 40, bred during winter) and summer group (n = 80, bred during summer) as well as, animals in the summer group were divided into first subgroup animals (n = 40) and injected with viteselen intramuscularly (15 ml) twice wee ... | 2008 | 18673331 |
| molecular characterization of follicle stimulating hormone receptor (fshr) gene in the indian river buffalo (bubalus bubalis). | follicle stimulating hormone (fsh) plays a central role in regulation of ovarian function in mammals. the actions of follicle stimulating hormone are mediated through receptors present on the granulosa cells of the ovary. in the present study we have cloned and characterized the fshr gene of buffalo. sequence analysis indicated that the buffalo fshr cdna sequence comprised of an open reading frame of 2085bp encoding a 695 amino acid protein. its nucleotide sequence showed more than 80% similarit ... | 2008 | 18675816 |
| an extended river buffalo (bubalus bubalis, 2n = 50) cytogenetic map: assignment of 68 autosomal loci by fish-mapping and r-banding and comparison with human chromosomes. | we report an extended river buffalo (bubalus bubalis, 2n = 50; bbu) cytogenetic map including 388 loci, of which 68 have been fish-mapped on autosomes in the present study. ovine and caprine bac clones containing both type i loci (known genes) and type ii loci (simple sequence repeats (srs), microsatellite marker, sequence-tagged sites (stss)), previously assigned to sheep chromosomes, have been localized on r-banded river buffalo chromosomes (bbu), which expands the cytogenetic map of this impo ... | 2008 | 18685962 |
| geo-referencing livestock farms as tool for studying cystic echinococcosis epidemiology in cattle and water buffaloes from southern italy. | cystic echinococcosis (ce), caused by the larval stages of the tapeworm echinococcus granulosus, is known to be one of the most important parasitic infection in livestock worldwide and one of the most widespread zoonoses known. in the present study, we used a geographical information system (gis) to study the spatial structure of livestock (cattle, water buffaloes and sheep) populations to gain a better understanding of the role of sheep as reservoir for the transmission of ce to cattle and wate ... | 2007 | 18686260 |
| prevalence of helminths in water buffaloes in hunan province, china. | the prevalence of helminths in the asian water buffaloes (bubalus bubalis) was investigated in hunan province, people's republic of china between april 2005 and october 2007. a total of 359 adult buffaloes slaughtered at local abattoirs in 12 representative geographical locations in hunan province were examined for the presence of helminths. the worms were examined, counted and identified to species according to existing keys and descriptions. a total of 13 helminth species were found representi ... | 2009 | 18704740 |
| purification and biochemical characterization of cytosolic glutathione-s-transferase from filarial worms setaria cervi. | the present study reports the purification and characterization of gst from cytosolic fraction of setaria cervi. gst activity was determined in various subcellular fractions of bovine filarial worms s. cervi (bubalus bubalis linn.) and was found to be localized mainly in the cytosolic and microsomal fractions. the soluble enzyme from s. cervi was purified to homogeneity using a combination of salt precipitation, centrifugation, cation exchange and gsh-sepharose affinity chromatography followed b ... | 2008 | 18752976 |
| the bovine genome. | in approximately 20 years, bovine genomics has progressed from synteny mapping of protein gene products to a nearly completed 7.5x whole genome sequence. the cattle genome map serves as a prototype for genomic studies in other bovids such as goats, sheep, and river buffalo in which chromosome arms are totally conserved at the current level of cytogenetic and map comparison. cattle genomics has contributed to the discovery of genes underlying economically important phenotypes including quantitati ... | 2006 | 18753770 |
| malignant catarrhal fever: a review. | malignant catarrhal fever (mcf) is a fatal lymphoproliferative disease of cattle and other ungulates caused by the ruminant gamma-herpesviruses alcelaphine herpesvirus 1 (alhv-1) and ovine herpesvirus 2 (ovhv-2). these viruses cause inapparent infection in their reservoir hosts (wildebeest for alhv-1 and sheep for ovhv-2), but fatal lymphoproliferative disease when they infect mcf-susceptible hosts, including cattle, deer, bison, water buffalo and pigs. mcf is an important disease wherever reser ... | 2009 | 18760944 |
| plasma and milk kinetic of eprinomectin and moxidectin in lactating water buffaloes (bubalus bubalis). | the pharmacokinetics and mammary excretion of moxidectin and eprinomectin were determined in water buffaloes (bubalus bubalis) following topical administration of 0.5mgkg(-1). following administration of moxidectin, plasma and milk concentrations of moxidectin increased to reach maximal concentrations (c(max)) of 5.46+/-3.50 and 23.76+/-16.63ngml(-1) at t(max) of 1.20+/-0.33 and 1.87+/-0.77 days in plasma and milk, respectively. the mean residence time (mrt) were similar for plasma and milk (5.2 ... | 2008 | 18774650 |
| effect of boron as an antidote on dry matter intake, nutrient utilization and fluorine balance in buffalo (bubalus bubalis) exposed to high fluoride ration. | it is well known that excessive accumulation of fluorides can exert toxic effects on various tissues and organs so as to severely damage the health and production of animals. the aim of this study was to determine beneficial effect of boron on nutrient utilization in buffalo calves exposed to high fluoride (f) ration. for this purpose, we used three groups of four male murrah buffalo calves (body weight 98-100 kg, aged 6-8 month) each. control animal was given only basal diet and concentrate mix ... | 2008 | 18781284 |
| hand-made cloned buffalo (bubalus bubalis) embryos: comparison of different media and culture systems. | hand-made cloning (hmc) has proved to be an efficient alternative to the conventional micromanipulator-based technique in some domestic animal species. this study reports the development of an effective culture system for in vitro culture of zona-free cloned buffalo (bubalus bubalis) embryos reconstructed using adult skin fibroblast cells as nucleus donor. cleavage and blastocyst rates observed were 52 and 0% in modified charles rosenkrans 2 (mcr2), 61 and 4.6% in modified synthetic oviductal fl ... | 2008 | 18800862 |
| influence of igf-i on buffalo (bubalus bubalis) spermatozoa motility, membrane integrity, lipid peroxidation and fructose uptake in vitro. | the objective of the present experiment was to examine the influence of mean physiological concentration of insulin-like growth factor-i (igf-i) on frozen-thawed surti buffalo (bubalus bubalis) spermatozoa functional parameters, i.e., motility, plasmalemma integrity, acrosomal integrity, functional membrane integrity, lipid peroxidation and fructose uptake in vitro. frozen-thawed semen samples (n=6) were washed in tris buffer and divided into two equal parts (control and igf-i groups). only in t ... | 2009 | 18804927 |
| mapping mhc genes in river buffalo. | the major histocompatibility complex (mhc) contains a set of genes necessary for antigen presentation in the immune system. this gene dense and polymorphic region of the mammalian genome is of considerable interest due to the role of mhc genes in immune function and animal health. previous cytogenetic studies have indicated that the mhc in river buffalo resides on the short arm of chromosome 2 (bbu2). a 5000-rad radiation hybrid mapping panel was recently generated to enable construction of a wh ... | 2008 | 18817324 |
| [immune-humoral response of water buffalo (bubalus bubalis) against anaplasma marginale (theiler, 1910)]. | the aim of the present study was to analyze the humoral-immune response of water buffalo (bubalus bubalis) naturally infected against anaplasma marginale. for this work, colostrums/milk and blood samples were sequentially collected from buffalo cows prior and after partum for a period of 335 days and from buffalo calves from birth to 365 days after. the antibodies in the colostrums/milk and serum samples of these animals were determined using an elisa indirect method and the data were analyzed a ... | 2008 | 18823574 |
| evaluation of molecular methods for the detection of brucella species in water buffalo milk. | brucellosis is a highly infectious disease affecting both animals and humans. the current standard tools for the diagnosis of this bacterial infection are serological and microbiological. in this study, we evaluated the feasibility of molecular assays as diagnostic tools for the detection of brucella spp. in water buffalo milk. for this purpose, we first compared different dna extraction protocols and pcr methods on artificially spiked milk samples. the most sensitive methods were then used to e ... | 2008 | 18832199 |
| [characterization and determination of nitrogen in preparation of qingkailing injection and its intermediate products]. | to analyze characterization and determination of nitrogen in the preparation of qingkailing injection and its intermediate products. | 2008 | 18841762 |
| factors affecting the quality of cryopreserved buffalo (bubalus bubalis) bull spermatozoa. | storage of buffalo (bubalus bubalis) bull semen in the cryopreserved state is discussed in this article. fertility rate in buffalo following artificial insemination with frozen-thawed semen is reviewed. to better understand the freezability of bubaline spermatozoa, the available data on biochemical components and the activity of specific enzymes of semen/spermatozoa are given. moreover, the major factors that may influence the post-thaw viability and fertility of buffalo spermatozoa are examined ... | 2009 | 18954384 |
| on water buffalo and academic medicine. | | 2008 | 18971670 |
| the domestic (water) buffalo in mainland tanzania. | | 2008 | 18975121 |
| cloning and expression of buffalo active chymosin in pichia pastoris. | to date, only recombinant chymosin has been obtained in its active form from supernatants of filamentous fungi, which are not as good candidates as yeasts for large-scale fermentations. since bos taurus chymosin was cloned and expressed, the world demand for this protease has increased to such an extent that the cheesemaking industry has been looking for novel sources of chymosin. in this sense because buffalo chymosin has properties that are more stable than those of b. taurus chymosin, it may ... | 2008 | 18975968 |
| antibiotic resistance and microbial composition along the manufacturing process of mozzarella di bufala campana. | the use of antibiotics as growth promoters in livestock, banned in all eu member states in january 2006, has led to selection of antibiotic resistant strains within environmental bacteria, including gram-positive, non pathogenic bacteria that colonize the gi tract of humans and animals. in italy and in other mediterranean countries, fermented foods employing environmental bacteria pre-existing in the raw substrates, rather than industrial starters of defined genotype, represent a significant pro ... | 2008 | 18990462 |
| lipid peroxidation, mitochondrial membrane potential and dna integrity of spermatozoa in relation to intracellular reactive oxygen species in liquid and frozen-thawed buffalo semen. | several factors affect sperm motility and functional integrity during preservation; the damage caused by reactive oxygen species (ros) being an important factor. the present study investigated intracellular ros generation and its relationship with sperm motility, lipid peroxidation (lpo), mitochondrial membrane potential (mmp) and dna integrity during preservation (liquid preservation at 4 degrees c and cryopreservation) of buffalo semen. fifty-six ejaculates, eight each from seven buffalo bulls ... | 2009 | 19010614 |
| application of dissociation curve analysis to radiation hybrid panel marker scoring: generation of a map of river buffalo (b. bubalis) chromosome 20. | fluorescence of dyes bound to double-stranded pcr products has been utilized extensively in various real-time quantitative pcr applications, including post-amplification dissociation curve analysis, or differentiation of amplicon length or sequence composition. despite the current era of whole-genome sequencing, mapping tools such as radiation hybrid dna panels remain useful aids for sequence assembly, focused resequencing efforts, and for building physical maps of species that have not yet been ... | 2008 | 19014630 |
| use of real-time pcr technique in studying rumen cellulolytic bacteria population as affected by level of roughage in swamp buffalo. | a real-time polymerase chain reaction approach was used in this study to determine the population of major ruminal bacterial species (fibrobacter succinogenes, ruminococcus albus, and ruminococcus flavefaciens) in digesta and rumen fluid of swamp buffalo (bubalus bubalis). four rumen-fistulated, male swamp buffalo were randomly assigned according to a 4 x 4 latin square design to evaluate the effect of the urea-treated rice straw (roughage source)-to-concentrate ratio on cellulolytic bacterial d ... | 2009 | 19018588 |
| populations of domesticated cattle and buffalo in the western forest complex of thailand and their possible impacts on the wildlife community. | the western forest complex (wefcom) of thailand is comprised of many protected areas and has one of the highest wildlife populations in the country. populations of wildlife in the wefcom have decreased dramatically over recent years. rapid economic development has resulted in the conversion of forest into agricultural and pastoral land, which has directly and indirectly impacted the wildlife community. this research aimed to evaluate populations of domesticated cattle (bos indicus) and buffalo ( ... | 2009 | 19036494 |