| chlamydia psittaci infection and reinfection in a veterinarian. | | 1999 | 10494396 |
| methods of detection of chlamydia psittaci in domesticated and wild birds. | to study the occurrence of chlamydia psittaci in domesticated and wild birds and compare the sensitivity of molecular detection with cell culture isolation. | 1999 | 10494398 |
| significance of host cell kinesin in the development of chlamydia psittaci. | the influence of the microtubule-associated motor protein kinesin on chlamydia psittaci inclusion development in epithelial and fibroblast cell lines was addressed. kinesin was blocked early after chlamydial internalization (4 h postinfection [p.i.]) and before the initiation of active chlamydial multiplication (8 h p.i.). chlamydia development was monitored by fluorescence and transmission electron microscopy at different times during the cycle. in both host cell lines, kinesin blockage restric ... | 1999 | 10496927 |
| expression of chlamydia psittaci- and human immunodeficiency virus-derived antigens on the cell surface of lactobacillus fermentum br11 as fusions to bspa. | the basic surface protein, bspa, has been used as a fusion partner to direct peptide antigens from the human immunodeficiency virus gp41 protein and the chlamydia psittaci ompa protein to the cell surface of lactobacillus fermentum br11. bspa has potential utility in the construction of live vaccines and diagnostic reagents. | 1999 | 10496936 |
| [seroprevalence of chlamydia psittaci in cats assembled in the center of zoonosis of the municipal of moron]. | | 1999 | 10509408 |
| turkeys are protected from infection with chlamydia psittaci by plasmid dna vaccination against the major outer membrane protein. | plasmid dna expressing the major outer membrane protein (momp) of an avian chlamydia psittaci serovar a strain has been tested for its ability to raise an immune response and induce protection against challenge with the same serovar. a combined parenteral (intramuscular injection) and mucosal route (dna drops administered to the nares) of dna inoculation was compared with gene gun-based immunization. the gene gun delivery of pcdna1/momp as well as the intramuscular-intranasal dna delivery primed ... | 1999 | 10540159 |
| differences in the immune response against ruminant chlamydial strains in a murine model. | cba/j mice were used in the present study to establish differences between the immune response to three chlamydial strains: ab7 (chlamydia psittaci wild-type strain), 1b (c. psittaci vaccinal strain) and ib1 (c. pecorum). the evolution of chlamydial infection was evaluated in each strain by studying the clinical signs, the number of bacteria isolated from the spleen and the pathology of the liver. three aspects of the immune response were then studied: the characterization of the infiltrate of l ... | 1999 | 10543384 |
| computational analysis of the polymorphic membrane protein superfamily of chlamydia trachomatis and chlamydia pneumoniae. | whole sequence genome analysis is invaluable in providing complete profiles of related proteins and gene families. the genome sequences of the obligate intracellular bacteria chlamydia trachomatis and chlamydia pneumoniae both encode proteins with similarity to several 90-kda chlamydia psittaci proteins. these proteins are members of a large superfamily, c. trachomatis with 9 members and c. pneumoniae with 21 members. all polymorphic membrane protein (pmp) are heterogeneous, both in amino acid s ... | 1999 | 10587946 |
| chlamydiosis in pen-raised bobwhite quail (colinus virginianus) and chukar partridge (alectoris chukar) with high mortality. | in a flock of 12,000 bobwhite quail (colinus virginianus) and 7200 chukar partridge (alectoris chukar), the owner had 100% morbidity and 40%-50% mortality in birds between the ages of 2 and 4 wk. affected birds were stunted and anorexic and had yellow/green diarrhea. two- and 4-wk-old birds submitted for necropsy all had slight nasal discharge. histopathologic examination revealed mild (bobwhite) to severe (chukar) rhinitis. immunohistochemistry was positive for chlamydia psittaci in all birds. ... | 1999 | 10611999 |
| microbial aetiology of community-acquired pneumonia in hospitalised patients. | adult patients hospitalised with community-acquired pneumonia were studied prospectively to determine the microbial aetiology of pneumonia. between april 1996 and march 1997, blood and sputum samples were collected for culture. throat swabs were obtained for isolation of viruses and for detection of antigens of chlamydia pneumoniae, influenza viruses a and b, respiratory syncytial virus and parainfluenza virus. antibodies against legionella spp., mycoplasma pneumoniae, chlamydia pneumoniae, chla ... | 1999 | 10614951 |
| [images in medicine. pseudo-tumoral form of chlamydia psittaci pneumonia]. | | 1999 | 10627228 |
| identification of an antigen localized to an apparent septum within dividing chlamydiae. | the process of chlamydial cell division has not been thoroughly investigated. the lack of detectable peptidoglycan and the absence of an ftsz homolog within chlamydiae suggest an unusual mechanism for the division process. our laboratory has identified an antigen (sep antigen) localized to a ring-like structure at the apparent septum within dividing chlamydial reticulate bodies (rb). antisera directed against sep show similar patterns of antigen distribution in chlamydia trachomatis and chlamydi ... | 2000 | 10639437 |
| selenium and vitamin e effect on antibody production of sheep vaccinated against enzootic abortion (chlamydia psittaci). | the effect of selenium (se) and vitamin e (vit e) on antibody production of sheep vaccinated against chlamydia psittaci (ovis) was investigated. thirty-two sheep, one year old, seronegative to chlamydia infection, vaccinated against enterotoxemia and dewormed were used. injectable sodium selenite (0.1 mg/kg b.w.) was given twice to animals of the first group (gse), with a three week interval. the sheep of the second group (ge) received 1 g vit e each orally, six times at weekly intervals. the an ... | 2000 | 10670702 |
| characterization of in vitro dna binding sites of the euo protein of chlamydia psittaci. | the euo gene of chlamydia is highly expressed early in the developmental cycle, relative to other genes, but continues to be expressed throughout the active growth phases. the precise function of euo protein is not known, but it binds to dna in vitro. in this study, we developed a selection and amplification scheme for identifying chlamydial genomic fragments to which euo preferentially binds in vitro. the scheme involved mixing recombinant euo with a chlamydia psittaci genomic library in a pblu ... | 2000 | 10678946 |
| polymorphonuclear neutrophils are necessary for the recruitment of cd8(+) t cells in the liver in a pregnant mouse model of chlamydophila abortus (chlamydia psittaci serotype 1) infection. | the role of polymorphonuclear neutrophils (pmns) in the development of the specific immune response against chlamydophila abortus (chlamydia psittaci serotype 1) infection was studied in a pregnant mouse model involving treatment with rb6-8c5 monoclonal antibody. pmn depletion significantly affected the immune response in the liver, in which the t-lymphocyte and f4/80(+) cell populations decreased, particularly the cd8(+) t-cell population. a th1-like response, characterized by high levels of ga ... | 2000 | 10679002 |
| touchdown enzyme time release-pcr for detection and identification of chlamydia trachomatis, c. pneumoniae, and c. psittaci using the 16s and 16s-23s spacer rrna genes. | three touchdown enzyme time release (tetr)-pcr assays were used to amplify different dna sequences in the variable regions of the 16s and 16s-23s spacer rrna genes specific for chlamydia trachomatis, chlamydia pneumoniae, and chlamydia psittaci as improved tests for sensitive diagnosis and rapid species differentiation. the tetr-pcr protocol used 60 cycles of amplification, which provided improved analytical sensitivity (0.004 to 0.063 inclusion-forming unit of chlamydia species per pcr). the se ... | 2000 | 10699002 |
| molecular characterization of a bacteriophage (chp2) from chlamydia psittaci. | comparisons of the proteome of abortifacient chlamydia psittaci isolates from sheep by two-dimensional gel electrophoresis identified a novel abundant protein with a molecular mass of 61.4 kda and an isoelectric point of 6.41. c-terminal sequence analysis of this protein yielded a short peptide sequence that had an identical match to the viral coat protein (vp1) of the avian chlamydiaphage chp1. electron microscope studies revealed the presence of a 25-nm-diameter bacteriophage (chp2) with no ap ... | 2000 | 10729119 |
| interaction between turkey monocytes and avian chlamydia psittaci in the presence of mycoplasma sp.: the importance of nitric oxide. | the interaction between chlamydia psittaci and turkey monocytes was studied in vitro. purified monocytes were inoculated with c. psittaci, in the presence or absence of mycoplasma hyorhinis. whereas turkey monocytes produced high amounts of nitric oxide (no) following the inoculation with m. hyorhinis, inoculation with c. psittaci did not induce no production in these phagocytes. the monocytes strongly supported chlamydial growth, as demonstrated by the presence of inclusion forming units, the p ... | 2000 | 10736525 |
| evaluation of a commercial test for antibodies to the chlamydial lipopolysaccharide (medac) for serodiagnosis of acute infections by chlamydia pneumoniae (twar) and chlamydia psittaci. | a commercial test (relisa) based on a recombinant chlamydial lipopolysaccharide (lps) antigen has been evaluated for the diagnosis of acute infections caused by chlamydia pneumoniae (twar) and chlamydia psittaci. this test and a microimmunofluorescence test (mif) were compared in 160 patients with community-acquired pneumonia. seventeen of nineteen cases with significant titre changes detected by relisa were confirmed by mif. the two remaining cases not confirmed by mif were considered false-pos ... | 2000 | 10737458 |
| echocardiographic follow-up of chlamydia psittaci myocarditis. | chlamydia psittaci myocarditis has been infrequently reported. a case of serologically confirmed c psittaci myocarditis with dilated left ventricle and severely impaired left ventricular function is described. serial echocardiograms demonstrated complete recovery after therapy. an early diagnosis has important prognostic implications. | 2000 | 10767264 |
| seroepidemiological investigation of feline chlamydiosis in cats and humans in japan. | the prevalence of chlamydia antibodies in japan was investigated in 215 cat sera, consisting of 88 sera of stray cats and 127 sera of pet cats, and 2,184 human sera, taken from 2,003 general persons and 181 small animal clinic veterinarians, by microimmunofluorescence (mif) testing with chlamydia psittaci fe/pn1 of feline origin and prk/6bc of avian origin as antigens. the prevalence rates of anti-fe/pn1 antibodies were 45.5% in stray cats, 17.3% in pet cats, 1.7% in general persons and 8.8% in ... | 2000 | 10789502 |
| chlamydia psittaci lipopolysaccharide. a reinvestigation of its chemical composition and structure. | a lipopolysaccharide (lps) of chlamydia psittaci pk 5082 strain associated with enzootic abortion in ewes was isolated from embryonated hen eggs-grown elementary bodies (ebs) by a phenol/water procedure. compositional analyses revealed the presence of 3-deoxy-d-manno-oct-2-ulosonic acid (kdo), glucosamine (glcn), phosphorus, and fatty acids in a molar ratio of 2.6:2.0:2.4:4.4. glcn was the only amino sugar detected. methylation analysis of the lps confirmed the presence of a kdo trisaccharide pr ... | 1999 | 10825928 |
| characterization of the rnpb gene and rnase p rna in the order chlamydiales. | the sequence of the rnase p rna gene (rnpb) was determined for 60 strains representing all nine species in the family chlamydiaceae and for the related chlamydiales species, parachlamydia acanthamoebae and simkania negevensis. these sequences were used to infer evolutionary relationships among the chlamydiaceae. the analysis separated chlamydophila and chlamydia into two lineages, with chlamydophila forming three distinct clusters: the chlamydophila pneumoniae strains; the chlamydophila pecorum ... | 2000 | 10826799 |
| virus serology in familial crohn disease. | recent findings that early-in-life virus infections represent a risk factor for crohn disease, that exacerbations of disease sometimes appear associated with common viral infections, and, in particular, suggestions that crohn disease may be the result of persistent infection with measles virus prompted serologic studies for antibody to 19 common viruses, chlamydia psittaci, and mycoplasma pneumoniae. | 2000 | 10831264 |
| microvirus of chlamydia psittaci strain guinea pig inclusion conjunctivitis: isolation and molecular characterization. | the authors report the isolation and molecular characterization of a bacteriophage, φcpg1, which infects chlamydia: psittaci strain guinea pig inclusion conjunctivitis. purified virion preparations contained isometric particles of 25 nm diameter, superficially similar to spike-less members of the φx174 family of bacteriophages. the single-stranded circular dna genome of φcpg1 included five large orfs, which were similar to orfs in the genome of a previously described chlamydia: bacte ... | 2000 | 10878129 |
| severe leukopenia and liver necrosis in young african grey parrots (psittacus erithacus erithacus) infected with psittacine circovirus. | this paper describes the signs, clinical pathology, and postmortem findings in 14 young african grey parrots (psittacus erithacus erithacus) that were naturally infected with psittacine beak and feather disease (pbfd) virus (psittacine circovirus). all but two of the parrots had severe leukopenia at clinical presentation. two other parrots also had severe anemia. all birds died within 3 wk after presentation. postmortem examination documented liver necrosis in 11 of 14 birds and secondary bacter ... | 2000 | 10879931 |
| psittacosis with increased gammadelta t cells in bronchoalveolar lavage fluid. | experience with two cases of psittacosis is described here in which the number of gammadelta t cell receptor-positive t lymphocytes (gammadelta t cell) in the bronchoalveolar lavage fluid was markedly increased (25.1 and 66.9%) and cd8+ t cells were also increased with reversal of the cd4/cd8 ratio. these values improved to the normal range along with recovery of their radiographical findings. the present findings suggest that gammadelta t cells may play an important role in protection from lung ... | 2000 | 10894106 |
| dynamics of the development of chlamydophila psittaci inclusions in epithelial and fibroblast host cells. | the development of chlamydophila psittaci (formerly chlamydia psittaci, avian strains) inclusions in fibroblast l-929 and epithelial bgm cell lines was studied along the bacterial growth cycle using a bgm cell-adapted strain in the presence or absence of cycloheximide and cycloheximide + polyethylene glycol. evolution of the inclusions was determined in terms of their number and size at 24, 30, 36, 48 and 54 h after infection. significant differences in the chlamydial growth were found between b ... | 2000 | 10900825 |
| compendium of measures to control chlamydia psittaci infection among humans (psittacosis) and pet birds (avian chlamydiosis), 2000. centers for disease control and prevention. | psittacosis--also known as parrot fever and ornithosis--is spread by a bacterial infection of birds that can cause severe pneumonia and other serious health problems among humans. from 1988 through 1998, 813 cases of psittacosis (infection with chlamydia psittaci) were reported to cdc, and most resulted from exposure to infected pet birds, usually cockatiels, parakeets, parrots, and macaws. in birds, c. psittaci infection is referred to as avian chlamydiosis (ac). infected birds shed the bacteri ... | 2000 | 10914931 |
| chlamydophila abortus in a brown skua (catharacta antarctica lonnbergi) from a subantarctic island. | on bird island, south georgia, a new strain of chlamydophila abortus was detected in one brown skua out of 37 specimens from six different seabird species. phylogenetic analysis of the rnpb and omp1 genes indicated the strain to be more closely related to c. abortus than to 6bc, the type strain of chlamydophila psittaci. | 2000 | 10919838 |
| isolation of chlamydia psittaci involved in abortion of goats in mexico: first report. | a group of 35 healthy adult goats ranging from two to six years old were bought from a chlamydiosis and brucellosis-free flock; they were vaccinated against brucella melitensis with rev1 vaccine at reduced doses, and one month later placed in isolation units. the animals were one month pregnant at the moment of purchase, and during the third month of pregnancy 10 out of the 35 dams aborted. necropsy of the aborted fetus and examination of the foetal membranes was performed where no macroscopic l ... | 1997 | 10932720 |
| ostrich diseases. | scientific knowledge of ostrich diseases is incomplete and very fragmented, with specific details on technical aspects of diagnostic and/or screening tests completely absent in most cases. salmonella typhimurium is common in multispecies collections and causes mortality in chicks younger than three months on commercial farms, but is rarely found in chicks older than six months, or slaughter birds of twelve to fourteen months in southern africa. campylobacter jejuni and chlamydia psittaci are occ ... | 2000 | 10935285 |
| recent epidemiological status of feline upper respiratory infections in japan. | epidemiology of upper respiratory infections of cats was studied. nasal, ocular, and oral swabs collected from 111 cats presented at animal hospitals during the past 2.5 years were examined. twenty-four (21.6%) and 4 (3.6%) cats were diagnosed as feline calicivirus (fcv) infection and feline viral rhinotracheitis, respectively, indicating fcv is more prevalent than feline herpesvirus-1, which revealed a considerable shift from data obtained in 1970s. cat sera immunized by using vaccines containi ... | 2000 | 10945306 |
| does inhibition of tumor necrosis factor alpha affect chlamydial genital tract infection in mice and guinea pigs? | the role of tumor necrosis factor alpha (tnf-alpha) in host defense against chlamydial infection remains unclear. in order to further evaluate the relevance of tnf-alpha to host resistance in chlamydial genital tract infection, we examined the effect of local inhibition of the tnf-alpha response in normal c57 mice and in interferon gamma gene-deficient c57 mice infected intravaginally with the mouse pneumonitis agent of chlamydia trachomatis. since the guinea pig model of female genital tract in ... | 2000 | 10948158 |
| phage infection of the obligate intracellular bacterium, chlamydia psittaci strain guinea pig inclusion conjunctivitis. | the infectious cycle of phicpg1, a bacteriophage that infects the obligate intracellular pathogen, chlamydia psittaci strain guinea pig inclusion conjunctivitis, was observed using transmission electron microscopy of phage-hyperinfected, chlamydia-infected hela cells. phage attachment to extracellular, metabolically dormant, infectious elementary bodies and cointernalisation are demonstrated. following entry, phage infection takes place as soon as elementary bodies differentiate into metabolical ... | 2000 | 10955956 |
| evaluation of strain-specific primer sequences from an abortifacient strain of ovine chlamydophila abortus (chalmydia psittaci) for the detection of eae by pcr. | strain-specific primer sequences derived from the helicase gene of an ovine abortifacient strain (s26/3) of chlamydophila abortus (chlamydia psittaci) were evaluated for the diagnosis of enzootic abortion in ewes (eae) by polymerase chain reaction (pcr). c abortus dna was amplified from tissues submitted from ovine abortion cases using genus-specific and strain-specific primers in a standard thermal cycler. amplification was followed by southern blotting and hybridisation with a strain-specific ... | 2000 | 10981698 |
| occurrence of chlamydiae in the genital tracts of sows at slaughter and their possible significance for reproductive failure. | the aim of this study was to investigate further the role of chlamydiae as pathogens in the genital tracts of sows at slaughter. genital tracts of 101 randomly selected sows were collected and specimens of genital tract localizations were systematically examined for chlamydiae using immunohistochemistry and pcr. in the genital tracts of 10 sows, chlamydia psittaci dna was detected by pcr, and was further typed as 'serotype 1' in nine cases and as avian strain 6 bc in one animal. however, all spe ... | 2000 | 11014069 |
| chlamydophila abortus (chlamydia psittaci serotype 1) clearance is associated with the early recruitment of neutrophils and cd8(+)t cells in a mouse model. | the immune mechanisms in response to chlamydophila abortus (chlamydia psittaci serotype 1) infection were studied in c57bl/6 and cba mice. the infection was monitored and the following aspects of the immune response were evaluated: the nature of the leucocyte infiltrate in the liver, the percentages of polymorphonuclear neutrophils (pmns), macrophages and lymphocytes in the spleen, and the concentrations of cytokines in serum. in addition, the serum concentrations of igg1 and igg2a were determin ... | 2000 | 11032671 |
| feline chlamydia and calicivirus infections. | feline conjunctivitis is common and often presents a clinical challenge to the veterinarian. chlamydia psittaci is an important pathogen and should always be considered when evaluating cats with conjunctivitis. fcv is an infrequent cause of conjunctivitis and only causes the disease in conjunction with other clinical signs of this infection, such as oral mucosal ulcers and upper respiratory tract disease. | 2000 | 11033872 |
| comparative analysis of the putative amino acid sequences of chlamydial heat shock protein 60 and escherichia coli groel. | the nucleotide sequences of the gene encoding chlamydial heat shock protein 60 (chsp60) of 7 chlamydia psittaci strains were determined. comparison of sequences of the chsp60 gene among chlamydiae showed high identities of the nucleotide sequences by 81.0% or greater and of the deduced amino acid sequences by 92.2% or greater. comparison of the amino acid sequences between chlamydia and the other bacterial hsp60s resulted in the finding of three highly conserved regions, suggesting that these re ... | 2000 | 11039588 |
| naturally occurring lesions of the uterine tube in sheep and serologic evidence of exposure to chlamydophila abortus. | the uterine tubes from 405 ewes, collected at an abattoir, were assessed grossly and microscopically for abnormalities that correlated with serological evidence of exposure to chlamydophila abortus. gross lesions were found in 41 ewes and 86 had microscopic lesions. enzyme immunoassay (eia) of serum was used as an indication of exposure of individual ewes to c. abortus; 52 were found to be positive. chi-squared analysis indicated no association between eia-positive animals and lesions of the ute ... | 2000 | 11041501 |
| [comparison of various diagnostic kits and antigens for the detection of chlamydial antibodies in the serum of patients with respiratory diseases] . | examination of sera of patients with respiratory diseases did not reveal any substantial difference in detection of chlamydial antibodies by elisa using two commercial kits (chlamydia stat and rrlisa) demonstrating igg antibodies and two corpuscular antigens prepared from chlamydia psittaci and chlamydia pneumoniae in the institute of virology, sas, in bratislava detecting whole serum antibodies. however, higher sensitivity of relisa diagnostic kit was found when analyzing immunoglobulin classes ... | 2000 | 11059109 |
| experimental enteric infection of gnotobiotic piglets with a chlamydia psittaci strain of avian origin. | the pathogenicity of a chlamydia psittaci isolate of pigeon origin was assessed using a litter of gnotobiotic piglets. at 3 days of age, six piglets were inoculated intragastrically with egg-grown chlamydiae, the remaining six pigs were sham-inoculated. the animals were observed for clinical signs, and they were killed and necropsied sequentially between 4 and 15 days of age. clinical manifestations consisted of slight softening of the faeces between 6 and 10 days post-inoculation (dpi). immunoh ... | 2000 | 11075544 |
| chlamydia species and mycoplasma pneumoniae. | chlamydia psittaci, chlamydia pneumoniae, and mycoplasma pneumoniae are a group of respiratory pathogens that have similar pulmonary and extrapulmonary manifestations. recent studies suggest that c. pneumoniae and m. pneumoniae may play a role in the pathogenesis of asthma, but further studies are needed to delineate the importance of these organisms in this disease. the diagnosis of c. pneumoniae infection is hindered by the lack of a gold standard: asymptomatic carriage of c. pneumoniae lowers ... | 1999 | 11095770 |
| the role of chlamydia in upper respiratory tract infections. | although chlamydia pneumoniae and chlamydia psittaci are well-established causes of community-acquired pneumonia, little is known about the role of chlamydia species in upper respiratory tract infections. c. pneumoniae may play a role in the pathogenesis of acute otitis media. although c. pneumoniae has been isolated from the middle-ear fluid of children with otitis, children in whom the organism was isolated from middle-ear fluid improved despite being treated with antibiotics that are not acti ... | 2000 | 11095846 |
| limited role of polymorphonuclear neutrophils in a pregnant mouse model of secondary infection by chlamydophila abortus (chlamydia psittaci serotype 1). | the aim of this work was to study the role of polymorphonuclear neutrophils (pmns) in the clearance of infection, and in the development of specific immunity against chlamydophila abortus (chlamydia psittaci serotype 1) secondary infection. a pregnant mouse model depleted of neutrophils by the rb6-8c5 monoclonal antibody was used. no clinical signs were observed in depleted or non-depleted mice after secondary infection and no significant differences were observed in the litter size between the ... | 2000 | 11095917 |
| [chlamydia infection and peripartum dilated cardiomyopathy in niger]. | peripartum cardiac failure due to cardiomyopathy is common in sub-saharan africa. the etiology is unknown. this study was performed in niger to assess a possible relationship between peripartum cardiomyopathy and chlamydia. a total of 50 african women presenting peripartum cardiomyopathy underwent testing for infection by chlamydia pneumoniae, chlamydia trachomatis, and chlamydia psittaci. the inclusion criteria were cardiac failure during the last three months of pregnancy or first 6 months pos ... | 2000 | 11100438 |
| pcr-based detection of chlamydial infection in swine and subsequent pcr-coupled genotyping of chlamydial omp1-gene amplicons by dna-hybridization, rflp-analysis, and nucleotide sequence analysis. | lung and intestine of 49 pigs with respiratory diseases and endocervical swabs from 205 sows with reproductive disorders were investigated for chlamydial infection by polymerase chain reaction. pcr primers targeted dna sequences on the chlamydial omp1 or omp2 genes. pcr amplicons were generated from 49.0% of pigs with respiratory disease, from 60.0% of sows with reproductive disorders, from 24.5% of respiratory healthy controls, but from no endocervical swabs from fertile sows. by dna hybridizat ... | 2000 | 11117968 |
| identification of protective epitopes by sequencing of the major outer membrane protein gene of a variant strain of chlamydia psittaci serotype 1 (chlamydophila abortus). | protective monoclonal antibodies (mabs) to the major outer membrane protein (momp) of species of the family chlamydiaceae, which is the primary vaccine candidate antigen, recognize nonlinear epitopes conferred by the oligomeric conformation of the molecule. protective mabs failed to recognize oligomeric momp of the variant strain llg, which bears amino acid substitutions in variable segments (vss) 1, 2, and 4, and competed with monomer-specific mabs mapping to these vss in reference strain 577. ... | 2001 | 11119563 |
| modulation of p2z/p2x(7) receptor activity in macrophages infected with chlamydia psittaci. | given the role that extracellular atp (atp(o))-mediated apoptosis may play in inflammatory responses and in controlling mycobacterial growth in macrophages, we investigated whether atp(o) has any effect on the viability of chlamydiae in macrophages and, conversely, whether the infection has any effect on susceptibility to atp(o)-induced killing via p2z/p2x(7) purinergic receptors. apoptosis of j774 macrophages could be selectively triggered by atp(o), because other purine/pyrimidine nucleotides ... | 2001 | 11121379 |
| [chest radiograph of atypical pneumonia: comparison among chlamydia pneumoniae. pneumonia, ornithosis, and mycoplasma pneumoniae pneumonia]. | no report has been found comparing chlamydia pneumoniae (c. pneumoniae) pneumonia radiographically with other atypical pneumonias, chlamydia psittaci (c. psittaci) pneumonia and mycoplasma pneumoniae (m. pneumoniae) pneumonia. we described the chest radiographs of three kinds of pneumonia cases: 46 cases of c. pneumoniae pneumonia, 39 cases of c. psittaci pneumonia, and 131 cases of m. pneumoniae pneumonia. radiographic shadows were categorized into main shadows and sub-shadows. the main shadows ... | 2000 | 11140079 |
| [lambing ewes as a source of severe psittacosis in a pregnant woman]. | the first documented dutch case of an ovine chlamydia psittaci infection concerned a 20-year-old woman in the 26th week of pregnancy, following contact with lambing sheep. she had a severe sepsis and had to be artificially ventilated. finally, the patient made a full recovery. the preterm born child died directly after birth. the placenta showed an acute intervillitis in which chlamydial antigen was demonstrated immunohistologically. infection with an ovine c. psittaci was confirmed by sequence ... | 2000 | 11155507 |
| field evaluation of a new commercially available elisa based on a recombinant antigen for diagnosing chlamydophila abortus (chlamydia psittaci serotype 1) infection. | a new commercially available elisa (elisar-chlamydia) for detecting antibodies against chlamydophila abortus has been evaluated using sheep field serum samples. the elisa is based on a recombinant antigen which expresses part of a protein from the 80-90kda family that is specific to c. abortus. sera (105) from six flocks with confirmed ovine chlamydial abortion (oea) outbreaks were used in this study, as well as sera (258) from 18 flocks which had suffered no oea in the last lambing. the elisar- ... | 2001 | 11165067 |
| diagnosis of ovine enzootic abortion using an indirect elisa (romp91b ielisa) based on a recombinant protein fragment of the polymorphic outer membrane protein pomp91b of chlamydophila abortus. | chlamydophila abortus is of major economic importance worldwide as one of the principal causes of abortion in sheep. serological diagnosis of infection by the complement fixation test (cft) is complicated by false positive reactions resulting from cross-reactive antibodies to chlamydophila pecorum. to improve diagnosis an indirect enzyme-linked immunosorbent assay (ielisa) based on a recombinant protein fragment of the c. abortus polymorphic outer membrane protein pomp91b (romp91b ielisa) was as ... | 2001 | 11179645 |
| a secondary structure motif predictive of protein localization to the chlamydial inclusion membrane. | chlamydiae are obligate intracellular pathogens that spend their entire growth phase sequestered in a membrane-bound vacuole called an inclusion. a set of chlamydial proteins, labelled inc proteins, has been identified in the inclusion membrane (im). the predicted inca, incb and incc amino acid sequences share very limited similarity, but a common hydrophobicity motif is present within each inc protein. in an effort to identify a relatively complete catalogue of chlamydia trachomatis proteins pr ... | 2000 | 11207561 |
| detection of chlamydial antibody by fetal serology--an aid to the diagnosis of ovine abortion. | two serological tests (indirect immunofluorescence and enzyme-linked immunosorbent assay) were developed for the detection of fetal antibody to chlamydia psittaci. fetal blood and thoracic fluid from 126 field cases of suspected ovine chlamydial abortion were examined using both tests. placenta and fetal tissues (lung, liver, and kidney) from the same animals were also examined by the following conventional diagnostic methods: isolation in mccoy cells, detection of chlamydial lipopolysaccharide ... | 2001 | 11243360 |
| identification of polymorphic outer membrane proteins of chlamydia psittaci 6bc. | the genomes of chlamydia spp. encode a family of putative outer membrane proteins, referred to as polymorphic outer membrane proteins (pomps), which may play a role in the avoidance of host immune defenses. we analyzed avian strain 6bc of chlamydia psittaci by polyacrylamide gel electrophoresis for the expression of pomps. at least six putative pomps were identified on the basis of their size (90 to 110 kda) and labeling with an outer membrane-specific probe, 3-(trifluoromethyl)-3-(m-[125i]iodop ... | 2001 | 11254603 |
| sphingomyelin trafficking in chlamydia pneumoniae-infected cells. | chlamydia pneumoniae is a bacterial obligate intracellular parasite with a developmental cycle common to all members of the genus chlamydia. like other chlamydiae, the developmental cycle of c. pneumoniae occurs entirely within a membrane-bound intracellular vacuole, termed an inclusion, that is non-fusogenic with endosomal or lysosomal compartments. to characterize the vesicular interactions of the c. pneumoniae inclusion, we used a fluorescent analogue of ceramide, (n-[7-(4-nitrobenzo-2-oxa-1, ... | 2001 | 11260137 |
| seroepizootiology of selected infectious disease agents in free-living birds of prey in germany. | four hundred forty-eight blood plasma samples from free-living birds of prey from berlin and the brandenburg area in eastern germany were tested for antibodies against newcastle disease virus (ndv), falcon herpesvirus (fhv), owl herpesvirus (ohv), and chlamydia psittaci. antibodies to ndv were detected in 6 (2%) of 346 tested diurnal birds of prey, whereas none of the owls (n = 55) was positive. the positive samples originated from two common buzzards (buteo buteo), three ospreys (pandion haliac ... | 2001 | 11272489 |
| chlamydial serology: comparative diagnostic value of immunoblotting, microimmunofluorescence test, and immunoassays using different recombinant proteins as antigens. | to improve the reliability of the serodiagnosis of chlamydia trachomatis infections, an immunoblot analysis, a microimmunofluorescence titration, and different immunoassays using synthetic peptides derived from species-specific epitopes in variable domain iv of the major outer membrane protein or recombinant antigens (heat shock protein 70 [hsp70], hsp60, hsp10, polypeptide encoded by open reading frame 3 of the plasmid [pgp3], macrophage infectivity potentiator, and a fragment of the total lipo ... | 2001 | 11283058 |
| detection of feline calicivirus, feline herpesvirus 1 and chlamydia psittaci mucosal swabs by multiplex rt-pcr/pcr. | a single tube, multiplex reverse transcription (rt)-polymerase chain reaction (pcr)/pcr assay was developed for detection of feline herpesvirus 1 (fhv1), chlamydia psittaci and feline calicivirus (fcv) in cats with upper respiratory tract disease (urtd), incorporating a simple, rapid extraction procedure capable of extracting both dna and rna. the assay was found to be as sensitive in vitro as simplex assays that have previously been shown to be as sensitive as, or more sensitive than, culture f ... | 2001 | 11376956 |
| peptide immunization of guinea pigs against chlamydia psittaci (gpic agent) infection induces good vaginal secretion antibody response, in vitro neutralization and partial protection against live challenge. | immunization of female guinea pigs with a chimeric peptide consisting of variable domain iv (vdiv) and a region known as gp8 from the major outer membrane protein of chlamydophila caviae, formerly chlamydia psittaci guinea pig inclusion conjunctivitis strain, was performed to assess whether humoral immune responses could be elicited in the reproductive tracts of immunized animals. the c. caviae strain is able to cause a sexually transmitted infection in the guinea pig that closely parallels c. t ... | 2001 | 11380677 |
| improved sensitivity of pcr for chlamydophila using pmp genes. | primers targeting the conserved pmp gene family of chlamydophila abortus were evaluated for their ability to improve the polymerase chain reaction (pcr) sensitivity. in purified dna, specific pmp primers (named cpsia and cpsib) allowed at least a 10-fold increase of the pcr sensitivity compared to the specific ompa primers for c. abortus, but also for c. psittaci and c. caviae strains. no amplification was observed on c. felis, c. pecorum, c. pneumoniae and chlamydia trachomatis strains. tested ... | 2001 | 11423206 |
| production of bartonella genus-specific monoclonal antibodies. | monoclonal antibodies (mabs) which react with heat-resistant proteins with molecular masses of 32 to 33 kda of 14 different bartonella species were produced. these antibodies did not react with antigens of 26 diverse bacterial strains by microimmunofluorescence assay except mab b3d4, which reacted with chlamydia psittaci and chlamydia trachomatis at low titers. the identification of a common bartonella antigenic protein will make it possible to later produce a diagnostic antigen by cloning and e ... | 2001 | 11427441 |
| [clinical comparison of chlamydia pneumoniae pneumonia, ornithosis, and mycoplasma pneumoniae pneumonia]. | to elucidate the differences between the clinical aspects of chlamydia pneumoniae (c. pn) pneumonia and those of two other atypical pneumonias, chlamydia psittaci (c. ps) pneumonia and mycoplasma pneumoniae (m. pn) pneumonia, we analyzed the symptoms and laboratory data on the cases of these three types of pneumonia: 46 cases of c. pn pneumonia, 39 cases of c. ps pneumonia, and 131 cases of m. pn pneumonia. c. pn pneumonia was significantly more frequent among the elderly (mean 70 +/- 16 years, ... | 2001 | 11431909 |
| genetic immunization for chlamydia psittaci. | plasmid dna expressing the major outer membrane protein (momp) of an avian chlamydia psittaci serovar a strain, has been tested for its ability to raise an immune response and induce protection against challenge with the same serovar. a combined parenteral (intramuscular injection) and mucosal route (dna drops administered to the nares) of dna inoculation was compared to gene gun-based immunization. the gene gun delivery of pcdna1/momp as well as the intramuscular-intranasal dna delivery primed ... | 2001 | 11436420 |
| endogenous interleukin-12 is not required for resolution of chlamydophila abortus (chlamydia psittaci serotype 1) infection in mice. | a th1 immune response involving gamma interferon (ifn-gamma) production is required to eliminate chlamydophila abortus infections. in this study, the role of interleukin-12 (il-12) in protecting against c. abortus infection was investigated using il-12(-/-) and wild-type (wt) c57bl/6 mice to determine the role of this th1-promoting cytokine. il-12(-/-) mice were able to eliminate the c. abortus infection in a primary infection. however, there was a delay in the clearance of bacteria when il-12(- ... | 2001 | 11447154 |
| association between chlamydia psittaci seropositivity and abortion in italian dairy cows. | although the seroprevalence of chlamydia psittaci is widespread in italian dairy herds, its role in inducing genital disorders has not been elucidated. we therefore set up a case-control study to compare seroprevalence to c. psittaci in an aborted-cow population and in a randomly selected control group in the province of parma (the po valley of northern italy). the true seroprevalence (45%) in aborted cows was significantly higher than that in the control group (24%) (adjusted odds ratio=2.53). | 2001 | 11448501 |
| experimental infection of pregnant ewes with enteric and abortion-source chlamydophila abortus. | two groups of pregnant ewes were experimentally infected oronasally in midpregnancy. a faecal and an abortion-source isolate of chlamydophila abortus were used. they were derived from a healthy ewe from a flock with no history of abortion, and from an aborted foetus in a farm with enzootic abortion. as assessed by modified ziehl-neelsen (mzn) staining, egg culture, antigen elisa, the clearview test and immunohistochemistry, inoculation resulted in placental and/or foetal infection in all ewes. h ... | 2001 | 11470549 |
| causes of caprine abortion: diagnostic assessment of 211 cases (1991-1998). | in an 8-year period, 1991-1998, 217 accessions of caprine abortions were submitted to the california veterinary diagnostic laboratory system. of these 217 submissions, 211 were suitable for examination in this study (6 had insufficient data). infectious agents as the cause of abortions were found in 37% of the cases: bacterial agents were identified in 30.5%, viral agents in 2%, fungal agents in 0.5%, and protozoal agents in 4% of the cases submitted. the most common causes of abortions were chl ... | 2001 | 11482610 |
| controlled study of the efficacy of clavulanic acid-potentiated amoxycillin in the treatment of chlamydia psittaci in cats. | twenty-four specific pathogen-free cats were inoculated with 3 x 10(3) infectious units of a field isolate of chlamydia psittaci on to the corneal surface. seven days later they were assigned randomly to three groups of eight and treated orally for 19 days with either clavulanic acid-potentiated amoxycillin, doxycycline or a placebo. both treated groups responded rapidly, with a marked reduction in isolation rates and clinical scores which were significantly lower than in the placebo group withi ... | 2001 | 11497397 |
| [diseases from chlamydia psittaci and chlamydia pneumoniae]. | | 2001 | 11515199 |
| chlamydial infections in small ruminants. | chlamydophila abortus (formerly chlamydia psittaci) is one of the most important causes of reproductive failure in sheep and goats, especially in intensively managed flocks. the disease is usually manifested as abortion in the last 2 to 3 weeks of gestation, regardless of when the animal was infected. ewes that abort are resistant to future reproductive failure due to c. abortus, but they become inapparent carriers and persistently shed the organism from their reproductive tracts during estrus. ... | 2001 | 11515403 |
| a role for tryptophan in immune control of chlamydial abortion in sheep. | tryptophan (trp) catabolism appears to be an important mechanism for regulation of inflammatory responses, resulting in t-cell tolerance and survival of semi-allogeneic concepti during pregnancy. trp catabolism can be induced by ifn-gamma, and is therefore an important host defence mechanism against intracellular pathogens. chlamydophila abortus is a bacterial pathogen that can cause persistent infection in non-pregnant sheep, but invades the placenta and causes abortion in late pregnancy. ifn-g ... | 2001 | 11557298 |
| labsystems enzyme immunoassay for chlamydia pneumoniae also detects chlamydia psittaci infections. | | 2001 | 11558474 |
| isolation of chlamydia psittaci from domestic cats with oculonasal discharge in japan. | eight strains of chlamydia psittaci were isolated in japan from the nasal and conjunctival swabs of six household cats using the l929 cell line of mouse fibroblast origin. the isolates were identified as c. psittaci on the basis of the formation of characteristic inclusion bodies in the cell culture detected by giemsa stain and immunofluorescence. comparison of nucleotide sequences of the ompa gene amplified from the three isolates with the published sequence of feline fepn strain of c. psittaci ... | 2001 | 11558555 |
| recombination in the ompa gene but not the omcb gene of chlamydia contributes to serovar-specific differences in tissue tropism, immune surveillance, and persistence of the organism. | sequences of the major outer membrane protein (momp) gene (ompa) and the outer membrane complex b protein gene (omcb) from chlamydia trachomatis, chlamydia pneumoniae, and chlamydia psittaci were analyzed for evidence of intragenic recombination and for linkage equilibrium. the sawyer runs test, compatibility matrices, and index of association analyses provided substantial evidence that there has been a history of intragenic recombination at ompa including one instance of interspecies recombinat ... | 2001 | 11567000 |
| amplified fragment length polymorphism differentiation between the vaccine strain chlamydia psittaci 1b and wild field strains. | | 2001 | 11583131 |
| seroprevalence of toxoplasma gondii and chlamydia psittaci in domestic pigeons (columbia livia domestica) at sebele, gaborone, botswana. | high antibody titres to toxoplasma gondii and chlamydophila psittaci were demonstrated in serum collected from domestic pigeons at average titres of 1:128 and 1:64 respectively. the public health implications of these findings are discussed. | 2001 | 11585096 |
| conjunctivitis due to chlamydophila felis (chlamydia psittaci feline pneumonitis agent) acquired from a cat: case report with molecular characterization of isolates from the patient and cat. | conjunctivitis due to chlamydiaceae other than chlamydia trachomatis is rarely reported because of infrequent occurrence or inadequate investigation. a case of chronic non-trachomatis chlamydial conjunctivitis is described. after full clinical information was supplied to the laboratory, a non-trachomatis chlamydia was recovered from the patient's eye. this organism, and a subsequent isolate from one of the patient's cats, were shown to be indistinguishable examples of the recently described spec ... | 2001 | 11597148 |
| in vitro and in vivo activities of sitafloxacin against chlamydia spp. | the in vitro and in vivo antichlamydial activity of sitafloxacin was investigated. the mics and minimal chlamydiacidal concentrations of sitafloxacin for various species of chlamydia ranged from 0.031 to 0.125 microg/ml. sitafloxacin had an excellent therapeutic effect on experimental chlamydia psittaci pneumonia and was more potent than tosufloxacin, ofloxacin, and ciproflxacin, although slightly less potent than sparfloxacin. | 2001 | 11600398 |
| protection conferred by commercially available vaccines against chlamydophila abortus in a mouse model. | | 2001 | 11700930 |
| [notification according to the law for protection against infections]. | since january 1st 2001, the law for protection against infections decrees the duty of notification along two tracks, on the one hand by notification of infectious diseases and on the other hand by reporting the pathogens. however, the names of pathogens change due to scientific progress. furthermore, these names include in some cases not only harmful agents of infectious diseases but also inoffensive organisms, i. e. giardia lamblia. there are also problems in the notification of other pathogens ... | 2001 | 11735068 |
| polymorphic outer-membrane proteins of chlamydophila abortus are glycosylated. | antigenic profiles of mono-, bi- and poly-specific monoclonal antibodies against 90 kda polymorphic outer-membrane proteins (pomps) and a 105 kda pomp-related protein of chlamydophila abortus atcc vr 656(t), after one- and two-dimensional electrophoretic analysis, helped identify each one of the triplets pomp 90, 91a and 91b, and a pomp-related protein at 85 kda. the lectin concanavalin a bound to the four pomps and the pomp-related protein in a specific manner and the binding was sensitive to t ... | 2001 | 11739762 |
| role of bcl-2 family members in caspase-independent apoptosis during chlamydia infection. | infection with an obligate intracellular bacterium, the chlamydia trachomatis lymphogranuloma venereum (lgv/l2) strain or the guinea pig inclusion conjunctivitis serovar of chlamydia psittaci, leads to apoptosis of host cells. the apoptosis is not affected by a broad-spectrum caspase inhibitor, and caspase-3 is not activated in infected cells, suggesting that apoptosis mediated by these two strains of chlamydia is independent of known caspases. overexpression of the proapoptotic bcl-2 family mem ... | 2002 | 11748163 |
| cross-reaction between the genus-specific lipopolysaccharide antigen of chlamydia spp. and the lipopolysaccharides of porphyromonas gingivalis, escherichia coli o119 and salmonella newington: implications for diagnosis. | seven hybridoma clones, secreting monoclonal antibodies (mabs) against the genus-specific chlamydial lipopolysaccharide (lps) antigen were obtained after immunization of balb/c mice with formalin killed chlamydia psittaci. the antigen-binding properties of the mabs were characterized in different immunologic reactions with purified chlamydial elementary bodies and lps antigens from s- and r-forms of gram-negative bacteria. four mabs reacted with the heterologous lps antigens of salmonella r-muta ... | 2001 | 11750161 |
| prevalence of chlamydophila abortus infection in domesticated ruminants in taiwan. | this study is to (1) investigate the prevalence of chlamydophila abortus infection in cows and goats in taiwan, and (2) compare the genetic properties of taiwanese isolates with abortion strains from other sources. approximately 71% of aborted cows and 58% of aborted does had igg against c. abortus in their sera. the seroprevalence rate in cows may be overestimated, because a certain degree of cross-reactivity with c. pecorum cannot be ruled out. only 22.7% (from aborted cows) and 33.3% (from ab ... | 2001 | 11767056 |
| mannose-receptor positive and negative mouse macrophages differ in their susceptibility to infection by chlamydia species. | it has been shown that n-linked high mannose type oligosaccharides competitively inhibits attachment to and infectivity of chlamydiae in hela cells. to further study whether mannose moieties are involved in the infectivity of chlamydiae, the susceptibility of mannose-receptor negative j774a and positive j774e mouse macrophages to chlamydia trachomatis, chlamydia psittaci and chlamydia pneumoniae was evaluated. c. trachomatis infected mannose-receptor positive cells better than mannose-receptor n ... | 2002 | 11782120 |
| [two cases of chlamydia psittaci infection occurring in employees of the same pet shop]. | we report here 2 cases of psittacosis in a pet shop. in the first case, a 44-year-old male was admitted with fever, and a chest radiograph showed an infiltration shadow in the right lower lung. one day later, a colleague of the first patient, a 42-year-old man, developed fever and was admitted. in this patient, chest radiography revealed an infiltration shadow in the left lower lung. both patients had mild liver dysfunction. the serum titer of a complement fixation (cf) test against chlamydia ps ... | 2001 | 11828730 |
| subspecies variation in greek strains of chlamydophila abortus. | the greek chlamydial strains fas, fag, vpg and llg, isolated from aborted sheep or goat foetuses, had been previously characterized as divergent on the basis of mouse cross-protection experiments, with llg and its homologous pos significantly different from the rest in inclusion morphology, polypeptide profiles and reactivity with monoclonal antibodies. to determine the genetic basis of their divergence the 16s-23s ribosomal intergenic spacer was analysed by rflp analysis of pcr 16sf2/23r amplic ... | 2002 | 11844621 |
| application of polymerase chain reaction for detection of legionella pneumophila in serum samples. | objective: to apply the polymerase chain reaction (pcr) to serum samples for the rapid diagnosis of legionnaire's disease using the l5sl9 and l5sr93 primers designed to generate a 104-base-pair (bp) fragment from the 5s rna gene of legionella spp. the amplified product was detected by electrophoresis and by hybridization with the l5s-1-specific probe. methods: single specimens of serum obtained from 24 patients with confirmed legionellosis, at different stages of their disease, were tested by pc ... | 1998 | 11864308 |
| prevalence of chlamydia trachomatis, chlamydia psittaci and chlamydia pneumoniae antibodies in blood donors and attendees of std clinics. | objective: to estimate the prevalence of chlamydia trachomatis, c. psittaci and c. pneumoniae antibodies in sera from altogether 931 blood donors, patients with symptoms of urethritis, assumed salpingitis and sexually acquired reactive arthritis (sara), and women with fertility problems. methods: igg antibodies to c. trachomatis, c. psittaci and c. pneumoniae were determined by microimmunofluorescence (mif) tests. all patients were also tested for genital c. trachomatis infection using direct im ... | 1996 | 11866775 |
| the clinical efficacy of topical and systemic therapy for the treatment of feline ocular chlamydiosis. | twenty-four specific-pathogen-free-derived cats aged four to 11 months were challenged by ocular application of a field isolate of chlamydia psittaci to evaluate the effect of topical and systemic therapy on the course of disease. the cats were monitored for 35 days post-challenge, with severity of clinical signs being measured using a scoring system, and ocular shedding of the organism monitored by culture of conjunctival swabs. all cats developed active c psittaci infection, and after 7 days t ... | 1999 | 11919013 |
| development and application of quantitative polymerase chain reaction assay based on the abi 7700 system (taqman) for detection and quantification of mycobacterium avium subsp. paratuberculosis. | numerous reports have described diagnostic methods based on the polymerase chain reaction (pcr) used to detect mycobacterium avium subsp. paratuberculosis, the causative agent of johne's disease. the result of conventional pcr tests has been only qualitative, either positive or negative; it does not present any quantitative information about the number of the agents in the specimen. a quantitative pcr method (is900 taqman) was developed to measure the number of m. a. paratuberculosis organisms p ... | 2002 | 11939333 |
| serological response of cattle to chlamydophila abortus in slovakia in 1996-2000. | in the slovak republic, in 1966-2000, 37,275 blood sera of cattle were investigated for the presence of antibodies against chlamydophila abortus using the method of complement fixation. the antibody occurrence had following tendency: in 1996--3.72%; 1997--10.02%; 1998--9.15%; 1999--15.99%; 2000--9.51% of the tested sera contained the antibodies. in most cases, antibodies in low titres, 1:32-1:64, were detected. positive serological reactions at such serum dilutions are not indicative of the clin ... | 2002 | 11944582 |
| isolation and characterisation of local strains of chlamydophila abortus (chlamydia psittaci serotype 1) from tunisia. | chlamydiosis is one of the major diseases that can lead to abortion in ewes. since 1997, in 5 regions of tunisia, chlamydia-related abortions have been reported in 15 sheep and goat flocks. one hundred and sixty-six sera and 50 vaginal swab samples were collected from adult ewes. chlamydial antigens were detected in 29 (58%) of the vaginal swabs using enzyme linked immunosorbent assay (elisa) while 9 (18%) were positive by cell culture. five strains were recovered from 4 different sheep flocks. ... | 2002 | 11944809 |
| gamma interferon fails to induce expression of indoleamine 2,3-dioxygenase and does not control the growth of chlamydophila abortus in bewo trophoblast cells. | the bewo trophoblast cell line does not constitutively express the tryptophan degrading enzyme indolamine 2,3-dioxygenase (ido), nor can ido expression be induced by gamma interferon. this correlates with the inability of bewo cells to control the growth of chlamydophila abortus, in contrast to effects observed in hela cells treated with gamma interferon. | 2002 | 11953413 |
| abortion in woman caused by caprine chlamydophila abortus (chlamydia psittaci serovar 1). | on a farm housing cattle and goats an abortion storm occurred affecting 50% of the goats during the lambing season 2000/2001. in one of three investigated caprine abortions chlamydophila abortus could be identified as aetiology. during this time a pregnant woman (pregnancy week 19/20) had contact with aborting goats. she developed a severe generalized infection and aborted. the placenta contained chlamydophila abortus shown by immunohistochemistry and pcr. the aim of the present case report is t ... | 2002 | 11971199 |
| biological properties and cell tropism of chp2, a bacteriophage of the obligate intracellular bacterium chlamydophila abortus. | a number of bacteriophages belonging to the microviridae have been described infecting chlamydiae. phylogenetic studies divide the chlamydiaceae into two distinct genera, chlamydia and chlamydophila, containing three and six different species, respectively. in this work we investigated the biological properties and host range of the recently described bacteriophage chp2 that was originally discovered in chlamydophila abortus. the obligate intracellular development cycle of chlamydiae has preclud ... | 2002 | 11976304 |
| chlamydial infection in canine atherosclerotic lesions. | we attempted to detect chlamydial antigens in canine atherosclerotic lesions from seven dogs by immunohistochemical technique using anti-chlamydia psittaci (c. psittaci) polyclonal and anti-c. pneumoniae monoclonal antibodies. immunopositive signals to both antibodies were recognized in the atherosclerotic lesions of the aortas, coronary and splenic arteries of all dogs. positive signals were found in the foamy cytoplasm of infiltrated macrophages and extracellular matrices in the lesions. in so ... | 2002 | 11996944 |