| three ferritin subunit analogs in chinese giant salamander (andrias davidianus) and their response to microbial stimulation. | ferritin, an evolutionarily conserved iron-binding protein, plays important roles in iron storage and detoxification and in host immune response to invading stimulus as well. in the present study, we identified three ferritin subunit analog cdnas from chinese giant salamander (andrias davidianus). all the three ferritin subunit cdnas had a putative iron responsive element in the 5'-untranslated region. two deduced ferritin subunits (designated as cgsferh and cgsferm) had the highest identity of ... | 2015 | 26319314 |
| induction of mild enterocolitis in zebrafish danio rerio via ingestion of vibrio anguillarum serovar o1. | vibrio anguillarum is the etiological agent of a fatal hemorrhagic disease known as vibriosis that affects a wide range of fish species, causing severe economic losses. several investigations have been carried out to elucidate the virulence mechanisms of this pathogen and to develop rapid detection techniques and effective disease-prevention strategies. the aim of our study was to evaluate the most effective way to induce mild enteritis in a fish model, in order to allow further applications. th ... | 2015 | 26119299 |
| comparative genomic and evolution of vertebrate nod1 and nod2 genes and their immune response in miiuy croaker. | the nucleotide-binding oligomerization domain proteins nod1 and nod2 are important cytoplasmic pathogen recognition receptors which sense microbial infections molecules to induce innate immune response. in this study, the sequence analysis showed that nod1 and nod2 genes in miiuy croaker (miichthys miiuy, mminod1 and mminod2) share some highly conserved motifs that crucial for recognizing the bacterial and viral components. quantitative expression analysis revealed mminod1 and mminod2 had the hi ... | 2015 | 26108036 |
| identification and expression analysis of irak1 gene in common carp cyprinus carpio l.: indications for a role of antibacterial and antiviral immunity. | in this study, the full-length complementary (c)dna of interleukin-1 receptor-associated kinase 1 gene (irak1) was cloned from common carp cyprinus carpio. the complete open reading frame of irak1 contained 2109 bp encoding a protein of 702 amino acid residues that comprised a death domain, a prost region, a serine-threonine-specific protein kinase catalytic domain and a c-terminal domain. the amino-acid sequence of c. carpio irak1 protein shared sequence homology with grass carp ctenopharyngodo ... | 2015 | 26099328 |
| larva of the greater wax moth, galleria mellonella, is a suitable alternative host for studying virulence of fish pathogenic vibrio anguillarum. | microbial diseases cause considerable economic losses in aquaculture and new infection control measures often rely on a better understanding of pathogenicity. however, disease studies performed in fish hosts often require specialist infrastructure (e.g., aquaria), adherence to strict legislation and do not permit high-throughput approaches; these reasons justify the development of alternative hosts. this study aimed to validate the use of larvae of the greater wax moth (galleria mellonella) to i ... | 2015 | 26099243 |
| quorum sensing determines the choice of antiphage defense strategy in vibrio anguillarum. | selection for phage resistance is a key driver of bacterial diversity and evolution, and phage-host interactions may therefore have strong influence on the genetic and functional dynamics of bacterial communities. in this study, we found that an important, but so far largely overlooked, determinant of the outcome of phage-bacterial encounters in the fish pathogen vibrio anguillarum is bacterial cell-cell communication, known as quorum sensing. specifically, v. anguillarum pf430-3 cells locked in ... | 2015 | 26081633 |
| a controllable bacterial lysis system to enhance biological safety of live attenuated vibrio anguillarum vaccine. | bacterial strains used as backbone for the generation of vaccine prototypes should exhibit an adequate and stable safety profile. given the fact that live attenuated vaccines often contain some potential risks in virulence recovery and spread infections, new approaches are greatly needed to improve their biological safety. here, a critically iron-regulated promoter pviua was screened from vibrio anguillarum, which was demonstrated to respond to iron-limitation signal both in vitro and in vivo. b ... | 2015 | 26052008 |
| involvement of relish gene from macrobrachium rosenbergii in the expression of anti-microbial peptides. | relish is an nf-kb transcription factor involved in immune-deficiency (imd) signal pathway. in this study, a relish gene (mrrelish) was identified from macrobrachium rosenbergii. the full length of mrrelish comprises 5072 bp, including a 3510 bp open reading frame encoding a 1169 bp amino acid protein. mrrelish contains a rel homology domain (rhd), a nucleus localization signal, an iκb-like domain (6 ankyrin repeats), and a death domain. phylogenetic analysis showed that mrrelish and other relis ... | 2015 | 26026243 |
| a manganese superoxide dismutase (mnsod) from ark shell, scapharca broughtonii: molecular characterization, expression and immune activity analysis. | manganese superoxide dismutase (mnsod) is one of the key members of the antioxidant defense enzyme family, however, data regarding to the immune function of mnsod in mollusks still remain limited now. in this study, a full-length mnsod cdna was identified by rapid amplification of cdna ends (race) method from cdna library of ark shell scapharca broughtonii (termed sbmnsod). the cdna contained an open reading frame (orf) of 696 bp which encoded a polypeptide of 232 amino acids, a 5'-utr with leng ... | 2015 | 25980798 |
| pathogenicity of members of the vibrionaceae family to cultured juvenile sablefish. | sablefish anoplopoma fimbria are a prized seafood species due to their high oil content and white flaky flesh. raising these species in culture can help to provide an important source of protein for humans and relief to declining wild fish populations. understanding the environmental factors that influence the production of sablefish is important for successful culturing. the significance of host-pathogen interactions in sablefish culture and the resulting environmental implications are unknown. ... | 2015 | 25970236 |
| profiling immune response in zebrafish intestine, skin, spleen and kidney bath-vaccinated with a live attenuated vibrio anguillarum vaccine. | | 2015 | 25956722 |
| dietary exposure to individual polybrominated diphenyl ether congeners bde-47 and bde-99 alters innate immunity and disease susceptibility in juvenile chinook salmon. | polybrominated diphenyl ethers (pbdes), used as commercial flame-retardants, are bioaccumulating in threatened pacific salmon. however, little is known of pbde effects on critical physiological functions required for optimal health and survival. bde-47 and bde-99 are the predominant pbde congeners found in chinook salmon collected from the pacific northwest. in the present study, both innate immunity (phagocytosis and production of superoxide anion) and pathogen challenge were used to evaluate h ... | 2015 | 25938634 |
| evolution of akirin family in gene and genome levels and coexpressed patterns among family members and rel gene in croaker. | akirins, which are highly conserved nuclear proteins, are present throughout the metazoan and regulate innate immunity, embryogenesis, myogenesis, and carcinogenesis. this study reports all akirin genes from miiuy croaker and analyzes comprehensively the akirin gene family combined with akirin genes from other species. a second nuclear localization signal (nls) is observed in akirin2 homologues, which is not in akirin1 homologues in all teleosts and most other vertebrates. thus, we deduced that ... | 2015 | 25912355 |
| vibriophages differentially influence biofilm formation by vibrio anguillarum strains. | vibrio anguillarum is an important pathogen in marine aquaculture, responsible for vibriosis. bacteriophages can potentially be used to control bacterial pathogens; however, successful application of phages requires a detailed understanding of phage-host interactions under both free-living and surface-associated growth conditions. in this study, we explored in vitro phage-host interactions in two different strains of v. anguillarum (ba35 and pf430-3) during growth in microcolonies, biofilms, and ... | 2015 | 25911474 |
| a c-type lectin fold gene from japanese scallop mizuhopecten yessoensis, involved with immunity and metamorphosis. | c-type lectins are a superfamily of ca(2+)-dependent carbohydrate-recognition proteins that are well known for their participation in pathogen recognition and clearance. in this study, a putative c-type lectin fold (myclf) gene was identified from the japanese scallop mizuhopecten yessoensis. the full-length of myclf was 645 bp, encoding a polypeptide of 167 amino acids. myclf carried a signal peptide of 20 amino acid residues, and a single carbohydrate recognition domain, having relatively high ... | 2015 | 25867372 |
| molecular characterization and functional analysis of a novel c-type lectin receptor-like gene from a teleost fish, plecoglossus altivelis. | c-type lectin-like receptors (clrs) are important pathogen pattern recognition molecules that recognize carbohydrate structures. however, the functions of these receptors in fish keep less known. in this study, we characterized a novel clr from a teleost fish, plecoglossus altivelis (ayu), tentatively named pacd209l. the cdna of pacd209l is 1464 nucleotides (nts) in length, encoding a polypeptide of 281 amino acid residues with a calculated molecular weight of 31.5 kda. multiple alignment of the ... | 2015 | 25842180 |
| relation between virulence of vibrio anguillarum strains and response to the host factors mucin, bile salts and cholesterol. | in this study, we investigated the responsiveness of 15 vibrio anguillarum strains to three host factors (mucin, bile salts and cholesterol). | 2015 | 25807847 |
| genome-wide snp identification for the construction of a high-resolution genetic map of japanese flounder (paralichthys olivaceus): applications to qtl mapping of vibrio anguillarum disease resistance and comparative genomic analysis. | high-resolution genetic maps are essential for fine mapping of complex traits, genome assembly, and comparative genomic analysis. single-nucleotide polymorphisms (snps) are the primary molecular markers used for genetic map construction. in this study, we identified 13,362 snps evenly distributed across the japanese flounder (paralichthys olivaceus) genome. of these snps, 12,712 high-confidence snps were subjected to high-throughput genotyping and assigned to 24 consensus linkage groups (lgs). t ... | 2015 | 25762582 |
| rescue of fish exposed to a lethal dose of pathogen, by signals from sublethally exposed survivors. | rainbow trout (oncorhynchus mykiss, walbaum) were challenged intraperitoneally with a sublethal dose of vibrio anguillarum vib1 and allowed to recover. then, after 7 days, naïve fish, (designated as 'bystander' fish) which had never been exposed to the pathogen, were introduced to the same tank. these swam with the adapted (recovered) fish for 7 days before both groups and a control (never exposed directly to the pathogen or to recovered fish) group were exposed to a lethal dose of vib1. mortali ... | 2015 | 25757731 |
| molecular characterization of the liver-expressed antimicrobial peptide 2 (leap-2) in a teleost fish, plecoglossus altivelis: antimicrobial activity and molecular mechanism. | liver-expressed antimicrobial peptide 2 (leap-2) is widespread in fish and plays an important role in the host's innate immune system. in this study, full-length cdna for leap-2 (paleap-2) gene was cloned and sequenced from ayu, plecoglossus altivelis. paleap-2 mrna was detected in a wide range of tissues, with the highest level of transcripts found in the liver. upon induction by vibrio anguillarum, its expression significantly increased in the liver, kidney, spleen, gill, and heart, but decrea ... | 2015 | 25749706 |
| effects of transportation stress and addition of salt to transport water on the skin mucosal homeostasis of rainbow trout (oncorhynchus mykiss). | transportation of live fish is a common practice among aquaculture facilities. many studies have previously reported how transport elicits physiological stress responses and increases disease susceptibility in farmed fish. the aim of this work is to investigate the changes that the skin of rainbow trout (oncorhynchus mykiss) experiences due to stress. since nacl is commonly added to transport water as a stress mitigator, the effects of salt addition on the skin mucosa and skin-associated bacteri ... | 2015 | 25705060 |
| comparison of immune response of pacific white shrimp, litopenaeus vannamei, after multiple and single infections with wssv and vibrio anguillarum. | our previous study demonstrated that pacific white shrimp (litopenaeus vannamei) infected by multiple pathogens showed higher mortality and death occurred more quickly than those infected by a single pathogen (jang et al., 2014). for better understanding the defense mechanism against white spot syndrome virus (wssv) and vibrio anguillarum, immune responses of shrimp were evaluated in this study. the mrna expression levels of five immune-related genes were analyzed by quantitative reverse real-ti ... | 2015 | 25700782 |
| identification of the atlantic cod l-amino acid oxidase and its alterations following bacterial exposure. | antibacterial factors that are present in epidermal mucus of fish have a potential role in the first line of host defence to bacterial pathogens. this study reports the identification of l-amino acid oxidase (lao) in atlantic cod (gmlao) and the changes in the molecule following bacterial exposure. the gmlao transcripts and lao activity were present on both the body surface and in the internal organs of the fish. relative mrna level of gmlao increased significantly in the gills, the spleen and t ... | 2015 | 25681742 |
| characterization of a gc1qr from the giant freshwater prawn, macrobrachium rosenbergii. | gc1qr, as a multicompartmental and a multifunctional protein, plays an important role in innate immunity. in this study, a gc1qr homolog (mrgc1qr) in the giant freshwater prawn, macrobrachium rosenbergii was identified. mrgc1qr, a 258-amino-acid polypeptide, shares high identities with gc1qr from other species. mrgc1qr gene was expressed in different tissues and was highest expressed in the hepatopancreas. in addition, the mrgc1qr transcript was significantly enhanced after 6 h of white spot syn ... | 2015 | 25555810 |
| use of gnotobiotic zebrafish to study vibrio anguillarum pathogenicity. | we evaluated the use of the gnotobiotic zebrafish system to study the effects of bacterial infection, and analyzed expression of genes involved in zebrafish innate immunity. using a gfp-labeled strain of vibrio anguillarum, we fluorescently monitored colonization of the zebrafish intestinal tract and used gene expression analysis to compare changes in genes involved in innate immunity between nongnotobiotic and gnotobiotic larvae. the experiments performed with the gnotobiotic zebrafish reveal n ... | 2015 | 25548877 |
| transcriptome analysis revealed changes of multiple genes involved in immunity in cynoglossus semilaevis during vibrio anguillarum infection. | half-smooth tongue sole (cynoglossus semilaevis) is one of the most valuable marine aquatic species in northern china. given to the rapid development of aquaculture industry, the c. semilaevis was subjected to disease-causing bacteria vibrio anguillarum. it therefore is indispensable and urgent to understand the mechanism of c. semilaevis host defense against v. anguillarum infection. in the present study, the extensively analysis at the transcriptome level for v. anguillarum disease in tongue s ... | 2015 | 25543033 |
| preliminary study on expression of antimicrobial peptides in european sea bass (dicentrarchus labrax) following in vivo infection with vibrio anguillarum. a time course experiment. | antimicrobial polypeptides (ampps) are humoral components of the vertebrates and invertebrates innate immune system. their potent broad spectrum antimicrobial activities have drawn the attention of the scientific community to their potential use not only as an alternative to antibiotics but also as functional targets for immunostimulants in order to enhance the host immunity. fish synthesize a great number of these peptides but in european sea bass, an important fish species in the mediterranean ... | 2015 | 25542381 |
| comparative genomic of the baff and baff-like genes and immune response to bacteria of miiuy croaker (miichthys miiuy). | as a member of the tumor necrosis factor (tnf) family, b cell activating factor (baff), also known as tnf ligand superfamily member 13b (tnf13b), playing a critical role in enhancing immune responses. baff is a central cytokine for b-cell survival, proliferation, maturation and immunoglobulin secretion. in the present study, we describe the identification of the miiuy croaker baff (designed mmbaff) and baff-like (designed mmbaff-like) genes. the cdna of mmbaff contains an open reading frame (orf ... | 2015 | 25542380 |
| identification, expression and immunological responses to bacterial challenge following vaccination of blt1 gene from turbot, scophthalmus maximus. | leukotriene b4 (ltb4) is well known as a chemoattractant for leucocytes, recent studies also showed its involvement in adaptive immunity. the purpose of this work is to report the cloning, characterization and gene expression of leukotriene b4 receptor (blt1) in turbot (scophthalmus maximus), as well as the immunological response to challenge following vaccination with a live attenuated vaccine vibrio anguillarum mvav6203. the full cdna sequence of turbot blt1 was cloned. the open reading frame ... | 2015 | 25541026 |
| identification and expression analysis of cdna encoding chitinase-like protein (clp) gene in japanese scallop mizuhopecten yessoensis. | chitinase-like proteins (clp) are important members of the glycoside hydrolase family 18 (gh18) and are involved in growth control and remodeling processes. in this study, a clp transcript was isolated and sequenced from the japanese scallop (mizuhopecten yessoensis) after screening expressed sequence tags. the full-length complementary dna of m. yessoensis clp (my-clp1) was 1555 bp in length, consisting of a 75-bp 5'-untranslated region (utr), a 160-bp 3'-utr, and a 1320-bp open reading frame b ... | 2014 | 25526193 |
| cloning and expression of a transcription factor activator protein-1 (ap-1) member identified from manila clam venerupis philippinarum. | the transcription factor activator protein-1 (ap-1) proteins are implicated to play a major role in the regulation of numerous genes involved in the function and development of the immune system, cell differentiation, proliferation, apoptosis, etc. it can bind to promoter of its target genes in a sequence-specific manner to transactivate or repress them. in this study, the full-length cdna of an ap-1 was identified from venerupis philippinarum (denoted as vpap-1) by est analysis and race approac ... | 2015 | 25523090 |
| identification and expression of antioxidant and immune defense genes in the surf clam mesodesma donacium challenged with vibrio anguillarum. | the immune system in marine invertebrates is mediated through cellular and humoral components, which act together to address the action of potential pathogenic microorganisms. in bivalve mollusks biomolecules implicated in oxidative stress and recognition of pathogens have been involved in the innate immune response. to better understand the molecular basis of the immune response of surf clam mesodesma donacium, qpcr approaches were used to identify genes related to its immune response against v ... | 2015 | 25481276 |
| impact of mucin, bile salts and cholesterol on the virulence of vibrio anguillarum towards gnotobiotic sea bass (dicentrarchus labrax) larvae. | in this study, we investigated the impact of the host factors mucin, bile salts and cholesterol on the virulence of the economically important aquatic pathogen vibrio anguillarum towards sea bass larvae. pretreatment of v. anguillarum with either one of the host factors (at 10 mg l(-1)) prior to inoculation into the sea bass rearing water increased virulence of the bacterium, although the effect of cholesterol was not significant. each of the three host factors significantly increased several vi ... | 2015 | 25465176 |
| structural characterization and expression analysis of a novel cysteine protease inhibitor from haliotis discus hannai ino. | the sequence of the cysteine protease inhibitor gene of haliotis discus hannai (designated hdcpi) was determined using the race method. the full-length hdcpi cdna is 1049 bp long, and contains an open reading frame of 813 bp, encoding a 271-amino-acid protein with a calculated molecular mass of 29.83 kda and an isoelectric point of 8.57. the deduced amino acid sequence of hdcpi contains two cystatin-like domains, and each has the structural features of the cystatin family, including three evolut ... | 2015 | 25463299 |
| molecular characterization of collagen iv evidences early transcription expression related to the immune response against bacterial infection in the red abalone (haliotis rufescens). | collagen iv has been described as a structural protein of the basement membrane, which as a whole forms a specialized extracellular matrix. recent studies have indicated a possible relationship between collagen iv and the innate immune response of invertebrate organisms. the present study characterized the alpha-1 chain of collagen iv in the red abalone haliotis rufescens (hr-coliv) and evaluated its association with the innate immune response against vibrio anguillarum. to further evidence the ... | 2015 | 25463284 |
| lect2 improves the outcomes in ayu with vibrio anguillarum infection via monocytes/macrophages. | leukocyte cell-derived chemotaxin 2 (lect2) is reported to be a cytokine involved in the immune response against pathogenic microorganisms in fish. however, its accurate function in whole fish remains unclear. in this study, we provide the first report on the effect of lect2 on fish defenses against pathogens in vivo. the administration of recombinant lect2 improved the survival rate of vibrio anguillarum infected ayu. the bacterial burden of v. anguillarum infected ayu was decreased in lect2-tr ... | 2014 | 25462453 |
| the tnfα converting enzyme (tace) from ayu (plecoglossus altivelis) exhibits tnfα shedding activity. | tnfα converting enzyme (tace) is responsible for converting membrane-anchored tnfα to its soluble form in mammalian. however, the function and characteristics of tace in teleosts is unclear. in this study, we report the cloning of a cdna sequence of the patace from ayu, plecoglossus altivelis. patace encodes an 865-aa polypeptide, which is closest to the tace gene found in pufferfish (takifugu rubripes). patace mrna was detected in all the tissues tested, although it was considerably higher in l ... | 2015 | 25458310 |
| effects of recombinant flagellin b and its nd1 domain from vibrio anguillarum on macrophages from gilthead seabream (sparus aurata l.) and rainbow trout (oncorhynchus mykiss, w.). | flagellin is the principal component of flagellum in gram negative and positive bacteria, and it is also the ligand that activates the toll-like receptor 5 (tlr5) in mammals and fish. in higher vertebrates, flagellin induces the activation of the membrane-bound tlr5 (tlr5m), which promotes the expression of proinflammatory cytokines and chemokines and the co-stimulatory molecules present in antigen-presenting cells needed for the activation of t cells. in the present study, we report the product ... | 2015 | 25449380 |
| increased survival of juvenile turbot scophthalmus maximus by using bacteria associated with cultured oysters. | preventing vibriosis in juvenile cultured turbot scophthalmus maximus caused by vibrio anguillarum frequently requires the use of feed supplemented with antibiotics in addition to vaccines. whether the use of probiotics instead of antibiotics in juvenile turbot is a safer strategy requires more study. the antibacterial potential of 148 vibrio spp. strains (mostly isolated from cultures of healthy oysters, clams, and turbot) was analyzed in vitro against v. anguillarum and other pathogens by mean ... | 2014 | 25361445 |
| rpos and indole signaling control the virulence of vibrio anguillarum towards gnotobiotic sea bass (dicentrarchus labrax) larvae. | quorum sensing, bacterial cell-to-cell communication with small signal molecules, controls the virulence of many pathogens. in contrast to other vibrios, neither the vani/vanr acylhomoserine lactone quorum sensing system, nor the three-channel quorum sensing system affects virulence of the economically important aquatic pathogen vibrio anguillarum. indole is another molecule that recently gained attention as a putative signal molecule. the data presented in this study indicate that indole signal ... | 2014 | 25360804 |
| the catecholamine stress hormones norepinephrine and dopamine increase the virulence of pathogenic vibrio anguillarum and vibrio campbellii. | obtaining a better understanding of mechanisms involved in bacterial infections is of paramount importance for the development of novel agents to control disease caused by (antibiotic resistant) pathogens in aquaculture. in this study, we investigated the impact of catecholamine stress hormones on growth and virulence factor production of pathogenic vibrios (i.e. two vibrio campbellii strains and two vibrio anguillarum strains). both norepinephrine and dopamine (at 100 μm) significantly induced ... | 2014 | 25264299 |
| a new group of anti-lipopolysaccharide factors from marsupenaeus japonicus functions in antibacterial response. | anti-lipopolysaccharide factors (alfs) are a group of critical effector molecules with a broad spectrum of antimicrobial activities in crustaceans. four groups of alfs (a, b, c, and d) have been identified in peneaid shrimp. in the study, we identified a new group of alfs (designated as mjalf-e) from marsupenaeus japonicus. this new group (group e) included mjalf-e1 and e2. mjalf-e1 was highly expressed in hemocytes, heart, and intestine, whereas e2 was highly expressed in gills, stomach, and in ... | 2015 | 25218642 |
| comparative genomic of the teleost cathepsin b and h and involvement in bacterial induced immunity of miiuy croaker. | cathepsins are a family of lysosomal proteases play different roles at physiological and pathological states and present in almost all animals as well as other organisms. cathepsins b and h are both cysteine proteases of cathepsins. cathepsin b and h have been studied playing parts in protein degradation/turnover, antigen presentation/processing and hormone maturation in mammals. however, little is known about the structures and functions of cathepsin b and h in teleosts. in the present study, w ... | 2014 | 25181651 |
| comparative genome sequencing to assess the genetic diversity and virulence attributes of 15 vibrio anguillarum isolates. | vibrio anguillarum is the causative agent of vibriosis, a deadly haemorrhagic septicaemic disease affecting various marine and fresh/brackish water fish, bivalves and crustaceans. however, the diversity and virulence mechanisms of this pathogen are still insufficiently known. in this study, we aimed to increase our understanding of v. anguillarum diversity and virulence through comparative genome analysis of 15 v. anguillarum strains, obtained from different hosts or non-host niches and geograph ... | 2015 | 25073650 |
| molecular cloning and characterization of secretory and membrane-bound igm of turbot. | in recent years, increasing diseases especially bacterial diseases have brought a host of losses with the expansive cultivation of turbot (scophthalmus maximus). in order to do more research about the immune system of turbot for better understanding the mechanism of resisting diseases, the immunoglobulin genes related to secretory and membrane-bound igm (s-igm and m-igm) of turbot were cloned using homology sequences cloning and smart race pcr method. the heavy chain of s-igm cdna is 1900 bp in ... | 2014 | 25066925 |
| lipopolysaccharide and β-1,3-glucan binding protein in the hard clam (meretrix meretrix): molecular characterization and expression analysis. | pattern recognition molecules play an important role in innate immunity by recognizing conserved molecular patterns that are present on the surface of invading microorganisms. in this study, a lipopolysaccharide and β-1,3-glucan binding protein (lgbp) gene was cloned from the hard clam meretrix meretrix (designated as mm-lgbp) by the expressed sequence tags and rapid amplification of cdna ends method. the cdna was 1827 bp in length, consisting of a 71-bp 5'-terminal untranslated region, a 62-bp ... | 2014 | 25062483 |
| development of a two-step, non-probed multiplex real-time pcr for surveilling vibrio anguillarum in seawater. | vibrio anguillarum is an aggressive and halophilic bacterial pathogen most commonly originating from seawater. vibrio anguillarum presence in fisheries and aquaculture facilities causes significant morbidity and mortality among aquaculture species primarily from haemorrhaging of the body and skin of the infected fish that eventually leads to death, collectively recognized as the disease vibriosis. this study served to develop a non-probe, multiplex real-time pcr assay to rapidly detect v. anguil ... | 2015 | 25039502 |
| characterization and expression pattern of a novel β-defensin in common carp (cyprinus carpio l.): implications for its role in mucosal immunity. | β-defensins are a group of cysteine-rich cationic antimicrobial peptides that play antibacterial and antiviral roles in immune systems of vertebrates. here, we report the cloning and identification of a β-defensin 3 cdna sequence from the common carp (cyprinus carpio l.). sequence alignment and phylogenetic analysis indicated that this β-defensin 3 belonged to the bd-2 group of fish. real-time pcr showed that the β-defensin 3 mrna was expressed in all the tissues of normal common carp that we ex ... | 2014 | 25036829 |
| iron shortage and bile salts play a major role in the expression of ompk gene in vibrio anguillarum. | the outer membrane protein k, ompk first identified in vibrio parahaemolyticus has been shown to be a receptor for a broad host range vibriophage kvp40 infecting members of the vibrionaceae. in the study, the effect of culture conditions on the expression of ompk in v. anguillarum was studied using real-time pcr. the expression increased significantly in the presence of bile salts and iron chelating agent 2, 2' bipyridine, suggesting a role for this protein in bile resistance and also in iron ac ... | 2014 | 25033659 |
| characterization and expression of sweetfish (pleco glossus altivelis) cathepsin d. | cathepsin d (ctsd) is a lysosomal acidic endoproteinase that plays an important role in immune response. in this study, we obtained sweetfish (plecoglossus altivelis) ctsd (pactsd) via de-novo transcriptome sequencing of sweetfish macrophages. the full length cdna sequence of pactsd was 1 955 bp encoding a propeptide of 397 amino acids. the deduced protein had a calculated molecular weight of 43.17 × 10³. multiple alignment with other known ctsd amino acid sequences revealed amino acid conservat ... | 2014 | 25017748 |
| complete genome sequence of vibrio anguillarum phage choed successfully used for phage therapy in aquaculture. | vibrio anguillarum phage choed was isolated from chilean mussels. it is a virulent phage showing effective inhibition of v. anguillarum. choed has potential in phage therapy, because it can protect fish from vibriosis in fish farms. here, we announce the completely sequenced genome of v. anguillarum phage choed. | 2014 | 25013148 |
| molecular characterization of a cxcl8-like protein from ayu and its effect on chemotaxis of neutrophils and monocytes/macrophages. | cxcl8, a cxc-type chemokine, plays a crucial role in acute inflammation by recruiting and mediating neutrophils and other cells. in this study, the cdna and genomic dna sequence of a cxcl8-like protein (pacxcl8l) from ayu (plecoglossus altivelis) was determined. sequence analysis showed that pacxcl8l represented the typical structure of animal cxcl8s. phylogenetic tree analysis indicated that pacxcl8l was closest to cxcl8 of atlantic cod (gadus morhua). constitutive expression of pacxcl8l was de ... | 2014 | 25010728 |
| notable mucosal immune responses induced in the intestine of zebrafish (danio rerio) bath-vaccinated with a live attenuated vibrio anguillarum vaccine. | live attenuated vaccine is one of the efficient vaccine candidates in aquaculture, which can be easily delivered to fish via bath-vaccination. an outstanding advantage of bath-vaccination is that vaccine delivery is through the same route as that utilized by many fish pathogens, generating specific mucosal immune responses. in this work, we investigated the mucosal immune responses induced by a live attenuated vibrio anguillarum vaccine in zebrafish via bath-vaccination. bacteria proliferated ra ... | 2014 | 24997435 |
| three representative subtypes of caspase in miiuy croaker: genomic organization, evolution and immune responses to bacterial challenge. | caspase proteins are intracellular proteases which function as initiators and effectors of apoptosis. according their difference of functions, the caspases can be divided into apoptosis related caspases and inflammatory mediator, and the former included apoptosis activator and apoptosis executioner. in this study, three different subtype caspases (caspase1, caspase3 and caspase9) from miichthys miiuy miiuy croaker were analyzed. the caspase1 belongs to the inflammatory mediator, caspase3 belongs ... | 2014 | 24973513 |
| a galectin with quadruple-domain from red abalone haliotis rufescens involved in the immune innate response against to vibrio anguillarum. | galectins are proteins that recognize and bind specifically β-galactosidase residues, playing important roles in the innate immune response of vertebrates and invertebrates. the cdna of a tandem repeat galectin from the red abalone haliotis rufescens cdna (hrgal) was cloned and characterized using rapid amplification of cdna end technique. the full-length cdna of hrgal was 2471 bp, with a 5' terminal untranslated region (utr) of 131 bp, a 3' utr of 672 pb, and an open reading frame (orf) of 1668 ... | 2014 | 24952088 |
| calnexin functions in antibacterial immunity of marsupenaeus japonicus. | calnexin (cnx) is an endoplasmic reticulum membrane-bound lectin chaperone that comprises a dedicated maturation system with another lectin chaperone calreticulin (crt). this maturation system is known as the cnx/crt cycle. the main functions of cnx are ca(2+) storage, glycoprotein folding, and quality control of synthesis. recent studies have shown that cnx is important in phagocytosis and in optimizing dendritic cell immunity. however, the functions of cnx in invertebrate innate immunity remai ... | 2014 | 24858031 |
| molecular characteristic and expression analysis of collagenolytic serine protease from the chinese mitten crab eriocheir sinensis with defense response to vibrio anguillarum challenge. | a novel collagenolytic serine protease (clsp) was cloned from the hemocytes of the chinese mitten crab eriocheir sinensis (es-clsp). the full-length cdna of es-clsp contains 990 nucleotides. it encodes a 270-amino acid-long peptide with the mature peptide containing 221 amino acids. it contains the conserved catalytic triad (h, d, and s). similarity analysis shows that es-clsp shares high identity with clsps from the fiddler crab uca pugilator. es-clsp mrna expression in e. sinensis is a) tissue ... | 2014 | 24841909 |
| immune response of apoptosis-related cysteine peptidases from the red abalone haliotis rufescens (hrcas8 and hrcas3): molecular characterization and transcription expression. | caspases play an important role in the different stages of programmed cell death, or apoptosis, which has been related to the immune response in multicellular organisms. the present study characterized an initiator caspase (hrcas8) and an effector caspase (hrcas3) from the red abalone haliotis rufescens using the race method and qpcr analysis. hrcas8 showed a complete sequence of 2529 base pairs (bp) with an open-reading frame (orf) of 1911 bp, a 5'utr of 201 bp, and a 3'utr of 417 bp. the estim ... | 2014 | 24821426 |
| identification, expression, and responses to bacterial challenge of the cathepsin c gene from the razor clam sinonovacula constricta. | cathepsin c (dipeptidyl-peptidasei, dppi) is a lysosomal cysteine proteinase that belongs to the papain superfamily, and it is involved in protein degradation and proenzyme activation. however, very little is known about the function of cathepsin c in bivalves. in the present study, we identified the cathepsin c gene in the razor clam sinonovacula constricta (sc-ctsc). the full-length sc-ctsc cdna contained a complete open reading frame (orf) of 1371 nt encoding 456 amino acids, a 98 bp 5' utr, ... | 2014 | 24792213 |
| identification of 21 novel immune-type receptors in miiuy croaker and expression pattern of three typical inhibitory members. | novel immune-type receptor (nitr) genes belong to the immunoglobulin superfamily and are encoded by clusters of multigene families. nitrs encode type i transmembrane proteins and are only found in teleosts. in the current study, total 21 nitr genes are identified from miiuy croaker (miichthys miiuy) and named as mmnitr1 to mmnitr21. miiuy croaker nitr genes that encoded one or two extracellular immunoglobulin (ig) domains, a transmembrane (tm) region, an immunoreceptor tyrosine-based inhibitor m ... | 2014 | 24704213 |
| host-induced increase in larval sea bass mortality in a gnotobiotic challenge test with vibrio anguillarum. | vibrio anguillarum is the major cause of haemorrhagic septicaemia, vibriosis, which is a severe disease affecting marine fish. in this work, it was found that the mortality of gnotobiotic sea bass larvae challenged with v. anguillarum was dependent on the number of dead fish in the vials at the moment of challenge. based on this finding, the effect of dead hosts (homogenised sea bass larvae or brine shrimp) on the virulence of v. anguillarum towards sea bass larvae was further investigated. addi ... | 2014 | 24695234 |
| the effect of vaccinating turbot broodstocks on the maternal immunity transfer to offspring immunity. | the study was designed to explore the effect of vaccinating turbot broodstocks on the immunity of offsprings. the expression of igm, c3, tgf-β1, il-1β, transferrin, lysozyme and their concentrations were detected respectively with quantitative real-time pcr and elisa kits in offsprings at different stages from both naive and vaccinated brood stocks. the survival rate of offsprings in pathogen challenge experiment and their pathogen inhibition rate were also detected. the results shown that the m ... | 2014 | 24657725 |
| a galectin from the kuruma shrimp (marsupenaeus japonicus) functions as an opsonin and promotes bacterial clearance from hemolymph. | galectins are a lectin family characterized by a conserved sequence motif in the carbohydrate recognition domain, which preferential binds to galactosyl moieties. however, few studies about the biological roles of galectins in invertebrates have been reported except for the galectin (cvgal1) from the eastern oyster crassostrea virginica. furthermore, galectins have been described in only a few crustacean species, and no functional studies have been reported so far. in this study, we identified a ... | 2014 | 24618590 |
| vibriophages and their interactions with the fish pathogen vibrio anguillarum. | vibrio anguillarum is an important pathogen in aquaculture, responsible for the disease vibriosis in many fish and invertebrate species. disease control by antibiotics is a concern due to potential development and spread of antibiotic resistance. the use of bacteriophages to control the pathogen may offer a non-antibiotic-based approach to reduce vibriosis. a detailed understanding of the phage-host interaction is needed to evaluate the potential of phages to control the pathogen. in this study, ... | 2014 | 24610858 |
| a novel lipopolysaccharide-binding protein (lbp) gene from sweetfish plecoglossus altivelis: molecular characterization and its role in the immune response of monocytes/macrophages. | lipopolysaccharide-binding protein (lbp) belongs to the lipid transfer/lbp (lt-lbp) family, and plays a crucial role in the recognition of bacterial components that modulate cellular signals in phagocytic cells. although several lbps have been identified in teleosts, the effects of lbp homologs on teleost phagocytic cells are still obscure. here, we report the cloning a novel full-length cdna sequence of lbp-like protein (palbp) gene from sweetfish, plecoglossus altivelis. the palbp cdna encoded ... | 2014 | 24594008 |
| upconversion fluorescent strip sensor for rapid determination of vibrio anguillarum. | here, we report a simple and ultrasensitive upconversion fluorescent strip sensor based on nayf4:yb,er nanoparticles (nps) and the lateral flow immunochromatographic assay (lfia). carboxyl-modified β-nayf4:yb,er nps were successfully synthesized by a facile one-pot solvothermal approach, upon further coupling with monoclonal antibody, the resultant ucnps-antibody conjugates probes were used in lfia and served as signal vehicles for the fluorescent reporters. v. anguillarum was used as a model an ... | 2014 | 24577708 |
| a genome scan for quantitative trait loci associated with vibrio anguillarum infection resistance in japanese flounder (paralichthys olivaceus) by bulked segregant analysis. | a recent genetic linkage map was employed to detect quantitative trait loci (qtls) associated with vibrio anguillarum resistance in japanese flounder. an f1 family established and challenged with v. anguillarum in 2009 was used for qtl mapping. of the 221 simple sequence repeat (ssr) markers used to detect polymorphisms in the parents of f1, 170 were confirmed to be polymorphic. the average distance between the markers was 10.6 cm. equal amounts of genomic dna from 15 fry that died early and fro ... | 2014 | 24562474 |
| th17-like immune response in fish mucosal tissues after administration of live attenuated vibrio anguillarum via different vaccination routes. | this study aimed to investigate the protective mucosal immunity elicited by live attenuated vibrio anguillarum in fish. zebrafish were immunized by bath or injection way, and undertook bath challenge at 28 days post vaccination. the results implied that bath vaccination was the better delivery route for inducing the protective immunity against bath challenge in zebrafish. the expressions of genes related to th1, th2 and th17 cells were measured in the mucosal tissues of vaccinated and challenged ... | 2014 | 24561130 |
| l-type lectin from the kuruma shrimp marsupenaeus japonicus promotes hemocyte phagocytosis. | l-type lectins (ltls) contain a luminal carbohydrate recognition domain, which exhibits homology to leguminous lectins. these type i membrane proteins are involved in the early secretory pathway of animals, and have functions in glycoprotein sorting, trafficking and targeting. recent studies suggest that ltls may be involved in immune responses in vertebrates, but no functional studies have been reported. this study reports an ltl, designated as mjltl1, from the kuruma shrimp marsupenaeus japoni ... | 2014 | 24508102 |
| deep transcriptome sequencing of pecten maximus hemocytes: a genomic resource for bivalve immunology. | pecten maximus, the king scallop, is a bivalve species with important commercial value for both fisheries and aquaculture, traditionally consumed in several european countries. major problems in larval rearing, however, still limit hatchery-based seed production. high mortalities during early larval stages, likely related to bacterial pathogens, represent the most relevant bottleneck. to address this issue, understanding host defense mechanisms against microbes is extremely important. in this st ... | 2014 | 24486903 |
| passive protective effect of chicken egg yolk immunoglobulins against experimental vibrio anguillarum infection in ayu (plecoglossus altivelis). | oral administration of chicken egg yolk immunoglobulins (igy) has attracted much attention as a means for controlling infectious diseases caused by microorganisms. this study evaluated the protective effect of igy against vibrio anguillarum infection in ayu, plecoglossus altivelis. igy was isolated from egg yolks laid by hens initially immunized with formalin-inactivated v. anguillarum. lower mortality of ayu was observed in groups treated with anti-v. anguillarum igy (avigy), compared with thos ... | 2014 | 24486630 |
| identification of two secreted ferritin subunits involved in immune defense of yesso scallop patinopecten yessoensis. | as an important iron storage protein, ferritin plays a crucial role in the iron-withholding defense system. in this study, two secreted ferritin subunits (pyfers1 and pyfers2) were identified from the yesso scallop, patinopecten yessoensis. the complete dna sequences of the two ferritins are 7101 and 5359 bp, consisting of seven and five exons, respectively. the full-length cdnas of pyfers1 and pyfers2 are 960 and 956 bp in length, encoding 228 and 220 amino acids, respectively. they have typica ... | 2014 | 24434645 |
| molecular characterization of leap-2 cdna in common carp (cyprinus carpio l.) and the differential expression upon a vibrio anguillarum stimulus; indications for a significant immune role in skin. | leap-2 is a cysteine-rich cationic antimicrobial peptide (amp) playing an important role in host innate immune system. leap-2 genes have been identified from higher vertebrates and several fish species. here we report the cloning and identification of two leap-2 cdna sequences from the liver of common carp (cyprinus carpio l.). the leap-2a cdna was 1325 bp long and contained an orf of 279 bp encoding a protein of 92 amino acids. the leap-2b cdna was 608 bp long and contained an orf of 276 bp enc ... | 2014 | 24418455 |
| antibacterial activity of serine protease inhibitor 1 from kuruma shrimp marsupenaeus japonicus. | serine protease inhibitors (serpins) are a large family of protease inhibitors involved in many critical biological processes such as blood coagulation, fibrinolysis, programmed cell death, development, and innate immunity. we identified mjserp1, a serpin in the kuruma shrimp marsupenaeus japonicus. the mjserp1 cdna has a 1239 bp open reading frame (orf) that encodes a 412-amino acid protein with a 23 aa signal peptide and a classic serpin domain. mjserp1 has a calculated molecular mass of 46.3 ... | 2014 | 24412215 |
| the responsive expression of a chitinase gene in the ridgetail white prawn exopalaemon carinicauda against vibrio anguillarum and wssv challenge. | chitinases are essential enzymes for crustaceans and participates in several biological processes, such as nutrient digestion, morphogenesis, pathogenesis, and pathogen defense. in the present study, the full-length cdna of chi (named ecchi) was cloned from the hemocytes of ridgetail white prawn exopalaemon carinicauda by rapid amplification of cdna ends methods. the full-length cdna of ecchi was 1,319 bp, including contains a 5'-untranslated region (utr) of 42 bp, 3'-utr of 101 bp with a poly ( ... | 2014 | 24408604 |
| molecular cloning and expression of irak-4, il-17 and i-κb genes in haliotis rufescens challenged with vibrio anguillarum. | the candidate genes interleukin-1 receptor associated kinase 4 (irak-4), interleukin 17 (il-17) and inhibitor of nf-κb (i-κb) were cloned and evaluated in californian abalone (haliotis rufescens) hemocytes in response to vibrio anguillarum. molecular characterization evidenced that hri-κb has a full cdna sequence of 3027 bp with an encoding region of 401 amino acids (aa), hrirak-4 comprised 1969 bp that encoded for 516 aa, and hr-il17 had a full sequence of 806 bp encoding for 165 aa. qpcr analy ... | 2014 | 24398261 |
| gene structure, immune response and evolution: comparative analysis of three 2-cys peroxiredoxin members of miiuy croaker, miichthys miiuy. | peroxiredoxin family was a superfamily of selenium independent peroxidases. it was divided into six subtypes: prx1-4 (typical 2-cys), prx5 (atypical 2-cys) and prx6 (1-cys). this study reports the isolation and characterization three 2-cys peroxiredoxin members of full cdna and genomic clones from miiuy croaker (miichthys miiuy). the genetic structure analysis showed that the c-terminal catalytic cys positioned within gevcpaxw. this sequence was different between prx3 and prx4, but was conservat ... | 2014 | 24378678 |
| a low-density lipoprotein receptor-related protein (lrp)-like molecule identified from chlamys farreri participated in immune response against bacterial infection. | low-density lipoprotein receptor-related protein (lrp) is a group of important endocytic receptors contributing to binding ligands and maintaining internal environment. in the present study, an lrp-like molecule was identified from zhikong scallop chlamys farreri (cflpr), and its mrna expression profiles, tissue location, and immunology activities were analyzed to explore its possible function in the innate immune system. the orf of cflrp was of 1971 bp encoding a polypeptide of 656 amino acids ... | 2014 | 24345370 |
| characterization and expression of the cxcr1 and cxcr4 in miiuy croaker and evolutionary analysis shows the strong positive selection pressures imposed in mammal cxcr1. | the innate immune system can recognize non-self, danger signals, and pathogen associated molecular patterns and provides a first line of antimicrobial host defense. therefore, it plays an instructive role and is pretty important in vertebrates. in innate immune responses, cxcrs act as the main receptors of cxc chemokines and play a vital role in host defense and inflammation. in present study, we cloned two cdna molecules of cxcr1 and cxcr4 in miichthys miiuy (miiuy croaker). in these two genes, ... | 2014 | 24333436 |
| toll-like receptor 22 of gilthead seabream, sparus aurata: molecular cloning, expression profiles and post-transcriptional regulation. | tlr22 is a fish-specific tlr that recognizes dsrnas. in the present study, a tlr22 homologue gene from gilthead seabream (sbtlr22) was identified and characterized. the full coding sequence contained a single open-reading frame of 2895 nucleotides encoding a predicted protein of 964 amino acids in length. its 3'-utr was relatively long, 1380 nucleotides, and contained three au-rich sequences frequently associated with mrna instability. functional studies showed that the sbtlr22 transcript had a ... | 2014 | 24333435 |
| identification and characterization of cynoglossus semilaevis microrna response to vibrio anguillarum infection through high-throughput sequencing. | micrornas (mirna) play key regulatory roles in diverse biological processes. cynoglossus semilaevis is an important commercial mariculture fish species in china. to identify mirnas and investigate immune-related mirnas of c. semilaevis, we performed high-throughput sequencing on three small rna libraries prepared from c. semilaevis immune tissues (liver, head kidney, spleen, and intestine). one library was prepared under normal conditions (control, cg); two were prepared during vibrio anguillaru ... | 2014 | 24296438 |
| the protein expression profile in hepatopancreas of scallop chlamys farreri under heat stress and vibrio anguillarum challenge. | heat stress and pathogen infection have been considered as the main causes for mass mortality of cultured scallops during summer. in the present study, the expression profiles of proteins in the hepatopancreas of scallop chlamys farreri were examined to reveal the possible mechanisms of physiological responses of scallop beneath heat stress and bacterial infection. an earlier occurred and higher mortality was observed in the scallops from combination treated group (28 °c and an injection of vibr ... | 2014 | 24262301 |
| molecular cloning and expression of a c-type lectin gene from venerupis philippinarum. | c-type lectins have been demonstrated to play important roles in invertebrate innate immunity by mediating the recognition of pathogens and clearing the micro-invaders. in the present study, a c-type lectin gene (denoted as vpctl) was identified from venerupis philippinarum by expressed sequence tag and rapid amplification of cdna ends approaches. the full-length cdna of vpctl consists of 904 nucleotides with an open-reading frame of 456 bp encoding a peptide of 151 amino acids. the deduced amin ... | 2014 | 24213925 |
| identification, characterization and functional analysis of a serine protease inhibitor (lvserpin) from the pacific white shrimp, litopenaeus vannamei. | as important arthropod immune responses, prophenoloxidase (propo) activation and toll pathway initiation are mediated by serine proteinase cascades and regulated by serpins. herein, a serine protease inhibitor (lvserpin), encoding for 415 amino acids with calculated molecular weight of 46,639 da and isoelectric point of 7.03 was characterized from the pacific white shrimp litopenaeus vannamei. multiple sequence alignment revealed that lvserpin shared the highest similarity with penaeus monodon s ... | 2014 | 24211341 |
| ecvig, a novel grouper immune-gene associated with antiviral activity against nnv infection. | vhsv-induced genes (vigs) were first identified in rainbow trout (oncorhynchus mykiss) and subsequently isolated in a variety of fish. recent studies have shown that most vigs have immunological functions against pathogenic infections. however, most research has focused on vig1, such that our present understanding of these genes in other fish species remains limited. this study isolated a homologue of the uncharacterized o. mykiss vig-b319 (ecvig) from orange-spotted grouper (epinephelus coioide ... | 2014 | 24211340 |
| molecular responses of calreticulin gene to vibrio anguillarum and wssv challenge in the ridgetail white prawn exopalaemon carinicauda. | calreticulin (crt), as a highly conserved endoplasmic reticulum luminal resident protein, plays important roles in ca(2+) homeostasis, molecular chaperoning and response to viral infection. in this study, a full-length cdna of crt (designated eccrt) was cloned from hemocytes of the ridgetail white prawn exopalaemon carinicauda by using rapid amplification of cdna ends (race) approaches. the full-length cdna of eccrt was 1725 bp, which contains a 5'-untranslated region (utr) of 57 bp, 3'-utr of 4 ... | 2014 | 24188748 |
| probiotics-pathogen interactions elicit differential regulation of cutaneous immune responses in epidermal cells of atlantic cod gadus morhua. | little is known on the cutaneous immune responses during probiotics-pathogen interactions in fish. thus, this study employed atlantic cod primary epidermal (ep) cell cultures as a model to understand this interaction. the probiotics-pathogen interactions in the ep cell cultures were elucidated using vibrio anguillarum 2133 (va) as the pathogen and two host-derived bacteria (gp21 and gp12) as the probiotics. there was a regional size difference on the ep cells; i.e., ep cells from the dorsal regi ... | 2014 | 24176817 |
| immune-related functions of the hivep gene family in east african cichlid fishes. | immune-related genes are often characterized by adaptive protein evolution. selection on immune genes can be particularly strong when hosts encounter novel parasites, for instance, after the colonization of a new habitat or upon the exploitation of vacant ecological niches in an adaptive radiation. we examined a set of new candidate immune genes in east african cichlid fishes. more specifically, we studied the signatures of selection in five paralogs of the human immunodeficiency virus type i en ... | 2013 | 24142922 |
| oral administration of formalin killed vibrio anguillarum cells improves growth and protection against challenge with vibrio harveyi in banana shrimp. | larval rearing in hatcheries and highly intensive grow-out culture practices followed in shrimp production systems favour the growth of potential pathogenic bacterial loads. this study reports the efficacy of formalin-killed vibrio bacterin on growth, survival and protection to challenge with virulent vibrio harveyi and vibrio anguillarum in juveniles of banana shrimp fenneropenaeus merguiensis. postlarvae 15 (0·24 ± 0·01 g) were administered orally in different concentrations of bacterial prepa ... | 2014 | 24138117 |
| a farnesoic acid o-methyltransferase (famet) from exopalaemon carinicauda is responsive to vibrio anguillarum and wssv challenge. | methyl farnesoate (mf), an analogue of the insect juvenile hormone iii, is believed to play important roles in the regulation of the growth and reproductive development in crustaceans. farnesoic acid o-methyltransferase (famet) is the key enzyme in the juvenile hormone biosynthetic pathway, involved in the conversion of farnesoic acid (fa) to mf in the final step of mf synthesis. in this study, a famet cdna (named ecfamet) was cloned from the hemocytes of ridgetail white prawn exopalaemon carini ... | 2014 | 24136172 |
| effect of multiple infections with white spot syndrome virus and vibrio anguillarum on pacific white shrimp litopenaeus vannamei (l.): mortality and viral replication. | multiple infections are commonly found in practical shrimp culture and may cause more serious consequences than infections by one pathogen only. therefore, this study was conducted to evaluate the effect of multiple infections with white spot syndrome virus (wssv) and vibrio anguillarum on pacific white shrimp litopenaeus vannamei (l.), mortality, wssv replication in vivo and host immune response. in the wssv single-infection group (wssv load, 2 × 10(2) copies μl(-1)), mean cumulative mortality ... | 2014 | 24127689 |
| a fibrinogen-related protein (tffrep2) gene involving in the immune response of trachidermus fasciatus against vibrio anguillarum. | fibrinogen-related proteins play important roles in the immune responses. we have obtained a cdna encoding a novel fibrinogen-related protein from roughskin sculpin trachidermus fasciatus (t. fasciatus) and named it as tffrep2. the n and c terminus of tffrep2 contain a putative 21-amino acid signal peptide and a typical 217-amino acid fibrinogen-like domain, which is conserved in all fibrinogen-related proteins. tffrep2 has three glycosylation sites and two potential calcium-binding sites that a ... | 2013 | 24091062 |
| complete genome sequence of vibrio anguillarum m3, a serotype o1 strain isolated from japanese flounder in china. | vibrio anguillarum is an important bacterial pathogen that causes vibriosis in marine fish. we present the complete genome sequence of v. anguillarum m3, a serotype o1 clinical strain isolated from japanese flounder (paralichthys olivaceus) in shandong, china. | 2013 | 24072867 |
| comparison of protein expression profiles of the hepatopancreas in fenneropenaeus chinensis challenged with heat-inactivated vibrio anguillarum and white spot syndrome virus. | fenneropenaeus chinensis (chinese shrimp) culture industry, like other penaeidae culture, has been seriously affected by the shrimp diseases caused by bacteria and virus. to better understand the mechanism of immune response of shrimp to different pathogens, proteome research approach was utilized in this study. firstly, the soluble hepatopancreas protein samples in adult chinese shrimp among control, heat-inactivated vibrio-challenged and white spot syndrome virus-infected groups were separated ... | 2014 | 24057166 |
| differential metabolic responses of clam ruditapes philippinarum to vibrio anguillarum and vibrio splendidus challenges. | clam ruditapes philippinarum is one of the important marine aquaculture species in north china. however, pathogens can often cause diseases and lead to massive mortalities and economic losses of clam. in this work, we compared the metabolic responses induced by vibrio anguillarum and vibrio splendidus challenges towards hepatopancreas of clam using nmr-based metabolomics. metabolic responses suggested that both v. anguillarum and v. splendidus induced disturbances in energy metabolism and osmoti ... | 2013 | 24056279 |
| vibrio anguillarum bacterin uptake via the gills of japanese flounder and subsequent immune responses. | the mucosal surfaces of fish allow for the introduction of foreign substances, including antigens, from the surrounding environment. in this study, uptake of vibrio anguillarum j-o-3 serotype bacterin by japanese flounder, and the subsequent immune responses were investigated. immunohistochemistry revealed that the bacterin was taken up through the epithelial cells of gills. the transcription levels of inflammatory cytokines such as interleukin (il)-1β, il-6 and tumor necrosis factor α were sign ... | 2013 | 24035752 |
| application of relative real-time pcr to detect differential expression of virulence genes in vibrio anguillarum under standard and stressed growth conditions. | in this study, we aimed to understand whether abiotic factors affect the expression of virulence genes in vibrio anguillarum. we observed the in vitro responses of two mediterranean strains of v. anguillarum to temperature, nacl and iron concentration changes. we monitored growth performance and gene transcription levels by comparing the results obtained under stressed conditions (temperatures of 5 °c, 15 °c and 37 °c; nacl concentrations of 3% and 5%; and iron depletion and excess) with those o ... | 2014 | 24033758 |
| clinical vibrio anguillarum infection in lumpsucker cyclopterus lumpus in scotland. | | 2013 | 24008994 |
| molecular cloning and transcriptional regulation of an allograft inflammatory factor-1 (aif-1) in zhikong scallop chlamys farreri. | the allograft inflammatory factor-1 (aif-1) is one of the key factors associated with inflammatory response. in the present study, the full-length cdna of aif-1 was identified from zhikong scallop chlamys farreri (named as cfaif-1) by est (expressed sequence tag) analysis and race (rapid-amplification of cdna ends) approaches. the cdna of cfaif-1 consisted of a 5-terminal untranslated region (utr) of 58 bp, a 3-utr of 607 bp with a poly (a) tail, and an open reading frame (orf) of 468 bp encodin ... | 2013 | 24001778 |
| does virulence assessment of vibrio anguillarum using sea bass (dicentrarchus labrax) larvae correspond with genotypic and phenotypic characterization? | vibriosis is one of the most ubiquitous fish diseases caused by bacteria belonging to the genus vibrio such as vibrio (listonella) anguillarum. despite a lot of research efforts, the virulence factors and mechanism of v. anguillarum are still insufficiently known, in part because of the lack of standardized virulence assays. | 2013 | 23936439 |