| heterodimeric nitrate reductase (napab) from cupriavidus necator h16: purification, crystallization and preliminary x-ray analysis. | the periplasmic nitrate reductase from cupriavidus necator (also known as ralstonia eutropha) is a heterodimer that is able to reduce nitrate to nitrite. it comprises a 91 kda catalytic subunit (napa) and a 17 kda subunit (napb) that is involved in electron transfer. the larger subunit contains a molybdenum active site with a bis-molybdopterin guanine dinucleotide cofactor as well as one [4fe-4s] cluster, while the small subunit is a di-haem c-type cytochrome. crystals of the oxidized form of th ... | 2007 | 17554176 |
| the impact of phb accumulation on l-glutamate production by recombinant corynebacterium glutamicum. | corynebacterium glutamicum, a gram-positive soil bacterium, has been used extensively for the industrial production of l-glutamate and other amino acids. in this study, an escherichia coli-c. glutamicum shuttle expression plasmid harboring polyhydroxybutyrate (phb) synthesis genes, phbcab from ralstonia eutropha, was constructed under the ptrc promoter. c. glutamicum harboring this plasmid accumulated 3-13% phb with a weight average molecular mass of 125,400 and a polydispersity of 11.3 when gro ... | 2007 | 17555841 |
| genomic characterization of the filamentous integrative bacteriophages {phi}rss1 and {phi}rsm1, which infect ralstonia solanacearum. | the genomic dna sequences were determined for two filamentous integrative bacteriophages, phirss1 and phirsm1, of the phytopathogen ralstonia solanacearum. the 6,662-base sequence of phirss1 contained 11 open reading frames (orfs). in the databases, this sequence showed high homology (95% identity) to the circular double-stranded dna plasmid pjtps1 (6,633 bp) isolated from a spontaneously occurring avirulent mutant of r. solanacearum. two major differences between the two sequences were observed ... | 2007 | 17557818 |
| fine structure and enzymatic degradation of poly[(r)-3-hydroxybutyrate] and stereocomplexed poly(lactide) nanofibers. | fiber morphology and crystalline structure of poly[(r)-3-hydroxybutyrate] (p(3hb)) and stereocomplexed poly(lactide) (pla) nanofibers were investigated by using scanning and transmission electron microscopies and x-ray and electron diffractions. in the p(3hb) nanofibers spun from less than 1 wt% 1,1,1,3,3,3-hexafluoro-2-propanol (hfip) solution, planar zigzag conformation (beta-form) as well as 2(1) helix conformation (alpha-form) structure was formed. based on the electron diffraction measureme ... | 2007 | 17561300 |
| emerging and unusual gram-negative infections in cystic fibrosis. | people with cystic fibrosis (cf) have chronic airway infection and frequent exposure to antibiotics, which often leads to the emergence of resistant organisms. in addition to the development of multiresistance in common cf pathogens such as pseudomonas aeruginosa, several newer, inherently resistant gram-negative species are becoming more common, including burkholderia cepacia complex, stenotrophomonas maltophilia, achromobacter (alcaligenes) xylosoxidans, certain ralstonia species, and those wi ... | 2007 | 17562501 |
| purification and characterization of a novel esterase (beta-hydroxypalmitate methyl ester hydrolase) and prevention of the expression of virulence by ralstonia solanacearum. | to screen novel micro-organisms and enzymes capable of degrading 3-hydroxypalmitic acid methyl ester (3-oh pame), the quorum-sensing signal molecule (quormone), which regulates the virulence of ralstonia solanacearum. | 2007 | 17584461 |
| bacterial community composition and structure of biofilms developing on nanofiltration membranes applied to wastewater treatment. | the structure and microbial communities of biofilms developing on cross-flow nanofiltration (nf) membranes at different temperatures (20, 25 or 34 degrees c) and operation lengths (8h-24days) were studied. feedwater comprised tertiary quality wastewater effluent or synthetic media mimicking effluents of intermediate quality. after each run, the membranes were autopsied for bacterial enumeration, bacterial community composition and microscopy visualization (sem, clsm and afm/nsom). community comp ... | 2007 | 17585989 |
| carbamoylphosphate serves as the source of cn(-), but not of the intrinsic co in the active site of the regulatory [nife]-hydrogenase from ralstonia eutropha. | within the catalytic centre of [nife]-hydrogenases one carbonyl and two cyanide ligands are covalently attached to the iron. to identify the metabolic origins of these ligands, the regulatory [nife] hydrogenase in conjunction with the indigenous hyp maturation proteins of ralstonia eutropha h16 were heterologously overproduced in e. coli grown in the presence of l-[ureido-(13)c] citrulline and nah(13)co(3). infrared spectroscopy of purified hydrogenase provided direct evidence that only the cyan ... | 2007 | 17599837 |
| comparative assessment of genetic diversity of peanut (arachis hypogaea l.) genotypes with various levels of resistance to bacterial wilt through ssr and aflp analyses. | bacterial wilt (bw) caused by ralstonia solanacearum is an important constraint to peanut (arachis hypogaea l.) production in several asian and african countries, and planting bw-resistant cultivars is the most feasible method for controlling the disease. although several bw-resistant peanut germplasm accessions have been identified, the genetic diversity among these has not been properly investigated, which has impeded efficient utilization. in this study, the genetic relationships of 31 peanut ... | 2007 | 17601614 |
| the plant pathogen ralstonia solanacearum needs aerotaxis for normal biofilm formation and interactions with its tomato host. | ralstonia solanacearum is a soilborne pathogen that causes bacterial wilt of diverse plant species. to locate and infect host plant roots r. solanacearum needs taxis, the ability to move toward more favorable conditions. however, the specific signals that attract this pathogen were unknown. one candidate is aerotaxis, or energy taxis, which guides bacteria toward optimal intracellular energy levels. the r. solanacearum genome encodes two putative aerotaxis transducers. cloned r. solanacearum aer ... | 2007 | 17601784 |
| combined effects of external mass transfer and biodegradation rates on removal of phenol by immobilized ralstonia eutropha in a packed bed reactor. | biodegradation of phenol by calcium-alginate immobilized ralstonia eutropha was carried out in a batch stirred and a packed bed reactor. in the batch system studies, the effect of initial phenol concentration on biodegradation was investigated at 30 degrees c and ph 7 while in the continuous system studies, the effects of flow rate and inlet phenol concentration on biodegradation were tested at the same temperature and ph. the observed biodegradation rate constant was calculated at different flo ... | 2008 | 17611023 |
| analysis of the contribution of the globin and reductase domains to the ligand-binding properties of bacterial haemoglobins. | bacterial hbs (haemoglobins), like vhb (vitreoscilla sp. hb), and flavohbs (flavohaemoglobins), such as fhp (ralstonia eutropha flavohb), have different autoxidation and ligand-binding rates. to determine the influence of each domain of flavohbs on ligand binding, we have studied the kinetic ligand-binding properties of oxygen, carbon monoxide and nitric oxide to the chimaeric proteins, fhpg (truncated form of fhp comprising the globin domain alone) and vhb-red (fusion protein between vhb and th ... | 2007 | 17617059 |
| natural transformation-based foreign dna acquisition in a ralstonia solanacearum muts mutant. | mutator strains with defective methyl-mismatch repair (mmr) systems have been shown to play an important role in adaptation of bacterial populations to changing and stressful environments. in this report, we describe the impact of muts::aacc3-iv inactivation on foreign dna acquisition by natural transformation in the phytopathogenic bacterium ralstonia solanacearum. a muts mutant of r. solanacearum exhibited 33- to 60-fold greater spontaneous mutation frequencies, in accordance with a mutator ph ... | 2007 | 17618086 |
| ralstonia pickettii bacteremia associated with pediatric extracorporeal membrane oxygenation therapy in a canadian hospital. | we describe 2 pediatric patients with ralstonia pickettii bacteremia associated with extracorporeal membrane oxygenation (ecmo) therapy. investigation revealed a common environmental source--the ecmo temperature-control units. we created guidelines for disinfecting these units that do not void the manufacturer's warranty and have prevented additional cases of bacteremia due to this organism. | 2007 | 17620255 |
| degradation of toluene by a mixed population of archetypal aerobes, microaerophiles, and denitrifiers: laboratory sand column experiment and multispecies biofilm model formulation. | an experiment was conducted in a saturated sand column with three bacterial strains that have different growth characteristics on toluene, pseudomonas putida f1 which degrades toluene only under aerobic conditions, thauera aromatica t1 which degrades toluene only under denitrifying conditions, and ralstonia pickettii pko1 has a facultative nature and can perform nitrate-enhanced biodegradation of toluene under hypoxic conditions (do <2 mg/l). steady-state concentration profiles showed that oxyge ... | 2008 | 17626295 |
| global virulence regulation networks in phytopathogenic bacteria. | phytopathogens coordinate multifaceted life histories and deploy stratified virulence determinants via complex, global regulation networks. we dissect the global regulation of four distantly related model phytopathogens to evaluate large-scale events and mechanisms that determine successful pathogenesis. overarching themes include dependence on centralized cell-to-cell communication systems, pervasive two-component signal-transduction systems, post-transcriptional regulation systems, arac-like r ... | 2007 | 17627825 |
| [isolation of phenol-degrading bacteria from natural soil and their phylogenetic analysis]. | five bacterial strains capable of utilizing phenol as sole carbon source for growth were isolated from non-contaminated natural soil sample after enrichment in the presence of phenol. they were preliminarily identified according to their phylogenetic analysis and physiological and biochemical characteristics. strain phd-2, phd-4 and phd-5 belonged to the genera of ralstonia, acinetobacter and microbacterium respectively; strain phd-1 and phd-3 were from the genus of pseudomonas. homology compari ... | 2007 | 17633645 |
| development of real-time pcr assays and evaluation of their potential use for rapid detection of burkholderia pseudomallei in clinical blood specimens. | the early initiation of appropriate antimicrobial therapy is critical for improving the prognosis of patients with septicemic melioidosis. thus, the use of a rapid molecular diagnosis may affect the outcome of this disease, which has a high mortality rate. we report the development of two taqman real-time pcr assays (designated 8653 and 9438) that detect the presence of two novel genes unique to burkolderia pseudomallei. the analytical sensitivity and specificity of the assays were assessed with ... | 2007 | 17634296 |
| phylogenetic and physiological diversity of dissimilatory ferric iron reducers in sediments of the polluted scheldt estuary, northwest europe. | the potential for dissimilatory ferric iron [fe(iii)] reduction in intertidal sediments of the polluted scheldt estuary, northwest europe, was assessed by combining field-based geochemical measurements with laboratory experiments on the associated microbiology. microbial communities at a freshwater and brackish location were characterized by culture-independent 16s rrna gene analysis, as well as enrichments, strain isolation and physiological screening. dilution-to-extinction batch enrichments u ... | 2007 | 17635542 |
| ralstonia mannitolilytica infection in renal transplant recipient: first report. | ralstonia mannitolilytica is being increasingly identified as an opportunist pathogen in immunocompromised patients. we report the first case of post renal transplant infection by r. mannitolilytica, in a 14-year-old recipient. the graft and the patient were saved with prompt microbiological identification, sensitivity testing and subsequent administration of appropriate antibiotic. | 2003 | 17643046 |
| bacterial contamination of water in dental unit reservoirs. | the aim of this study was bacteriological assessment of water in dental unit reservoirs--concentration and composition of the aerobe and facultative anaerobe bacterial microflora. reservoir water samples were taken from 25 units. bacterial flora were determined with the plate culture method. bacteria were identified with biochemical microtests: api 20e, api 20ne (biomérieux, france) and gp2 microplatetm (biolog, usa). the concentration of total bacteria isolated from one site was 201,039 cfu/ml, ... | 2007 | 17655191 |
| new bacteriophages that infect the phytopathogen ralstonia solanacearum. | four kinds of bacteriophage (phirsl, phirsa, phirsm and phirss) were isolated from ralstonia solanacearum, a soil-borne gram-negative bacterium that is the causative agent of bacterial wilt in many important crops. the myovirus-type phages phirsl1 and phirsa1 contained dsdna genomes of 240 kbp and 39 kbp, respectively. these phages have relatively wide host ranges and gave large clear plaques with various host strains; especially phirsa1 was able to infect all 15 r. solanacearum strains of diffe ... | 2007 | 17660427 |
| evaluation of quantitative polymerase chain reaction to assess nosz gene prevalence in mixed microbial communities. | the usefulness of quantitative polymerase chain reaction (qpcr) to measure nosz gene prevalence in a multi-template reaction was assessed by comparing 19 nosz template dna samples and 91 model communities. efficiencies of the qpcr varied but were not significantly different among nosz genotypes and were not linked to genetic distance from ralstonia eutropha. nosz genotype qpcr efficiencies obtained from isolated denitrifiers were higher (84.8%) than those obtained from excised denaturing gradien ... | 2007 | 17668022 |
| mechanical failure of a level 1 rapid infuser as a potential source of septicaemia. | | 2007 | 17669547 |
| "intelligent" descriptions of microbial kinetics in finitely dispersed bioreactors: neural and cybernetic models for phb biosynthesis by ralstonia eutropha. | abstract: | 2007 | 17686143 |
| metabolism of chlorinated biphenyls: use of 3,3'- and 3,5-dichlorobiphenyl as sole sources of carbon by natural species of ralstonia and pseudomonas. | ralstonia sp. sa-3, ralstonia sp. sa-4 and pseudomonas sp. sa-6 are natural strains with a novel capacity to utilize meta-substituted dichlorobiphenyls (dicbs) hitherto not known to serve as a sole source of carbon and energy for polychlorobiphenyl-degraders. in growth experiments, axenic cultures of isolates grew logarithmically on 3,3'-dicb with generation times that ranged insignificantly (t-test, p>0.05) from 30.4 to 33.8 h. both 3-chlorobenzoate (3-cba) and chloride produced as metabolites ... | 2008 | 17706746 |
| genomic analysis reveals widespread occurrence of new classes of copper nitrite reductases. | recently, the structure of a cu-containing nitrite reductase (nir) from hyphomicrobium denitrificans (hdnir) has been reported, establishing the existence of a new family of cu-nir where an additional type 1 cu (t1cu) containing cupredoxin domain is located at the n-terminus (nojiri et al. in proc. natl. acad. sci. usa 104:4315-4320, 2007). hdnir retains the well-characterised coupled t1cu-type 2 cu (t2cu) core, where the t2cu catalytic site is also built utilising ligands from neighbouring mono ... | 2007 | 17712582 |
| identification and localization of cyanophycin in bacteria cells via imaging of the nitrogen distribution using energy-filtering transmission electron microscopy. | in this study the technique of energy-filtering transmission electron microscopy was applied to localize cyanophycin (cgp) in recombinant strains of ralstonia eutropha. since cgp is a polymer consisting of the amino acids aspartate and arginine, which functions as a temporary nitrogen reserve that is deposited as insoluble inclusions in the cytoplasm of the cell, its nitrogen content is significantly higher than that of the other cell matter. in this study, we recorded nitrogen distribution maps ... | 2007 | 17713945 |
| characterization of hbze-encoded gentisate 1,2-dioxygenase from pseudomonas alcaligenes ncimb 9867. | pseudomonas alcaligenes ncimb 9867 (strain p25x) is known to synthesize two isofunctional gentisate 1,2-dioxygenases (gdo; ec 1.13.11.4) as well as other enzymes involved in the degradation of xylenols and cresols via the gentisate pathway. the hbze gene encoding what is possibly the strictly inducible gentisate 1,2-dioxygenase ii (gdo-ii) was cloned, overexpressed and purified as a hexahistidine fusion protein from escherichia coli. active recombinant gdo-ii had an estimated molecular mass of 1 ... | 2007 | 17720458 |
| isolated poly(3-hydroxybutyrate) (phb) granules are complex bacterial organelles catalyzing formation of phb from acetyl coenzyme a (coa) and degradation of phb to acetyl-coa. | poly(3-hydroxybutyrate) (phb) granules isolated in native form (nphb granules) from ralstonia eutropha catalyzed formation of phb from (14)c-labeled acetyl coenzyme a (coa) in the presence of nadph and concomitantly released coa, revealing that phb biosynthetic proteins (acetoacetyl-coa thiolase, acetoacetyl-coa reductase, and phb synthase) are present and active in isolated nphb granules in vitro. nphb granules also catalyzed thiolytic cleavage of phb in the presence of added coa, resulting in ... | 2007 | 17720797 |
| ralstonia solanacearum strains from martinique (french west indies) exhibiting a new pathogenic potential. | we investigated a destructive pathogenic variant of the plant pathogen ralstonia solanacearum that was consistently isolated in martinique (french west indies). since the 1960s, bacterial wilt of solanaceous crops in martinique has been caused primarily by strains of r. solanacearum that belong to either phylotype i or phylotype ii. since 1999, anthurium shade houses have been dramatically affected by uncharacterized phylotype ii strains that also affected a wide range of species, such as helico ... | 2007 | 17720825 |
| the duf81 protein taue in cupriavidus necator h16, a sulfite exporter in the metabolism of c2 sulfonates. | the degradation of taurine, isethionate and sulfoacetate in cupriavidus necator (ralstonia eutropha) h16 was shown by enzyme assays to be inducible, and each pathway involved sulfoacetaldehyde, which was subject to phosphatolysis by a common sulfoacetaldehyde acetyltransferase (xsc, h16_b1870) to yield acetyl phosphate and sulfite. the neighbouring genes encoded phosphate acetyltransferase (pta, h16_b1871) and a hypothetical protein [domain of unknown function (duf)81, h16_b1872], with eight der ... | 2007 | 17768248 |
| detection and visualization of an exopolysaccharide produced by xylella fastidiosa in vitro and in planta. | many phytopathogenic bacteria, such as ralstonia solanacearum, pantoea stewartii, and xanthomonas campestris, produce exopolysaccharides (epss) that aid in virulence, colonization, and survival. eps can also contribute to host xylem vessel blockage. the genome of xylella fastidiosa, the causal agent of pierce's disease (pd) of grapevine, contains an operon that is strikingly similar to the x. campestris gum operon, which is responsible for the production of xanthan gum. based on this information ... | 2007 | 17827325 |
| chaperones specific for the membrane-bound [nife]-hydrogenase interact with the tat signal peptide of the small subunit precursor in ralstonia eutropha h16. | periplasmic membrane-bound [nife]-hydrogenases undergo a complex maturation pathway, including cofactor incorporation, subunit assembly, and finally twin-arginine-dependent membrane translocation (tat). in this study, the role of the two accessory proteins hoxo and hoxq in the maturation of the membrane-bound [nife]-hydrogenase (mbh) of ralstonia eutropha h16 was investigated. mbh activity was absent in soluble as well as membrane fractions of cells with deletions in the respective genes. the ab ... | 2007 | 17850259 |
| influence of native microbiota on survival of ralstonia solanacearum phylotype ii in river water microcosms. | ralstonia solanacearum phylotype ii biovar 2 causes bacterial wilt in solanaceous hosts, producing severe economic losses worldwide. waterways can be major dissemination routes of this pathogen, which is able to survive for long periods in sterilized water. however, little is known about its survival in natural water when other microorganisms, such as bacteriophages, other bacteria, and protozoa, are present. this study looks into the fate of a spanish strain of r. solanacearum inoculated in wat ... | 2007 | 17873071 |
| spectroscopic insights into lead(ii) coordination by the selective lead(ii)-binding protein pbrr691. | | 2007 | 17880216 |
| monoclonal outbreak of catheter-related bacteraemia by ralstonia mannitolilytica on two haemato-oncology wards. | ralstonia mannitolilytica is a non-fermentative, gram-negative bacterium isolated infrequently from clinical samples. however, within a period of 11 weeks five inpatients of the tertiary care hospital of the university of tübingen developed clinical signs of infection and r. mannitolilytica was cultivated from blood samples of all patients suggesting an outbreak. | 2007 | 17881058 |
| isolation of the acrylamide denitrifying bacteria from a wastewater treatment system manufactured with polyacrylonitrile fiber. | acrylamide has carcinogenicity and toxicity, so its discharge to natural water and soil systems might have an adverse impact on water quality, endangering public health and welfare. the investigation attempts to isolate acrylamide denitrifying bacteria from a wastewater treatment system manufactured with polyacrylonitrile (pan) fiber. the goal is to elucidate the effectiveness of isolated pure strain and pan mixed strains in treating acrylamide from synthetic wastewater. the results reveal that ... | 2007 | 17882504 |
| treatment of arsenic contaminated water in a laboratory scale up-flow bio-column reactor. | the present paper describes the observations on the treatment of arsenic contaminated synthetic industrial effluent in a bio-column reactor. ralstonia eutropha mtcc 2487 has been immobilized on the granular activated carbon (gac) bed in the column reactor. the synthetic water sample containing as(t) (as(iii):as(v)=1:1), fe, mn, cu and zn at the initial concentrations of 25, 10, 2, 5, 10 ppm, respectively, was used. concentrations of all the elements have been found to be reduced below their perm ... | 2008 | 17890001 |
| the ralstonia solanacearum pathogenicity regulator hrpb induces 3-hydroxy-oxindole synthesis. | the transcriptional activator hrpb of the bacterial wilt causing betaproteobacterium ralstonia solanacearum represents a key regulator for pathogenicity. in particular, it drives expression of hrp genes encoding a type iii secretion system (t3ss) as well as effector molecules for delivery into the host cytosol to promote disease. however, the hrpb regulon extends beyond this t3ss. we describe here an hrpb-activated operon of six genes that is responsible for the synthesis of a fluorescent isatin ... | 2007 | 17890323 |
| ralstonia pickettii in environmental biotechnology: potential and applications. | xenobiotic pollutants such as toluene and trichloroethylene are released into the environment by various industrial processes. ralstonia pickettii possess significant biotechnological potential in the field of bioremediation and has demonstrated the ability to breakdown many of these toxic substances. here, we provide a description of the major compounds that various strains of r. pickettii are capable of degrading and a brief review of their breakdown pathways and an argument for its use in bio ... | 2007 | 17897177 |
| horizontal gene transfer regulation in bacteria as a "spandrel" of dna repair mechanisms. | horizontal gene transfer (hgt) is recognized as the major force for bacterial genome evolution. yet, numerous questions remain about the transferred genes, their function, quantity and frequency. the extent to which genetic transformation by exogenous dna has occurred over evolutionary time was initially addressed by an in silico approach using the complete genome sequence of the ralstonia solanacearum gmi1000 strain. methods based on phylogenetic reconstruction of prokaryote homologous genes fa ... | 2007 | 17957239 |
| mobility and recalcitrance of organo-chromium(iii) complexes. | hexavalent chromium [cr(vi)] is a major industrial pollutant. bioremediation of cr(vi) to cr(iii) is a viable clean-up approach. however, cr(vi) bioreduction also produces soluble organo-cr(iii) complexes, and little is known about their behavior in the environment. when tested with soil columns, citrate-cr(iii) showed little sorption to soil; malate-cr(iii) had limited partitioning with soil; and histidine-cr(iii) exhibited significant interaction with soil. it appears that the mobility varies ... | 2008 | 17959226 |
| detection of potential transgenic plant dna recipients among soil bacteria. | the likelihood of gene transfer from transgenic plants to bacteria is dependent on gene number and the presence of homologous sequences. the large number of transgene copies in transplastomic (transgenes contained in the chloroplast genome) plant cells as well as the prokaryotic origin of the transgene, may thus significantly increase the likelihood of gene transfer to bacteria that colonize plant tissues. in order to assess the probability of such transfer, the length of homologous dna sequence ... | 2007 | 17961481 |
| assessment of transformability of bacteria associated with tomato and potato plants. | transformation of plant-associated bacteria by plant dna has never been demonstrated in agricultural fields. in total 552 bacterial isolates from stems of ralstonia solanacearum-infected and healthy tomato plants and from stems and leaves of healthy potato plants were tested for natural genetic competence using plasmid psktg dna and homologous dna extracts. control strain acinetobacter baylyi adp1 was transformable with both dna extracts. no transformable isolates were observed after treatment w ... | 2007 | 17961482 |
| purification and molecular cloning of an intracellular 3-hydroxybutyrate-oligomer hydrolase from paucimonas lemoignei. | an intracellular 3-hydroxybutyrate-oligomer hydrolase was purified from a poly(3-hydroxybutyrate)-degrading bacterium, paucimonas lemoignei. it hydrolyzed the 3-hydroxybutyrate dimer with the highest specific activity of any of the enzymes reported so far. the gene was cloned and sequenced. the deduced amino acid sequence showed that the enzyme is a homolog of the phazc of ralstonia eutropha h16. | 2007 | 17964488 |
| genomic characterization of ralstonia solanacearum phage phirsa1 and its related prophage (phirsx) in strain gmi1000. | phirsa1 is a wide-host-range bacteriophage isolated from ralstonia solanacearum. in this study, the complete nucleotide sequence of the phirsa1 genomic dna was determined. the genome was 38,760 bp of double-stranded dna (65.3% g+c) with 19-bp 5'-extruding cohesive ends (cos) and contained 51 open reading frames (orfs). two-thirds of the phirsa1 genomic region encodes the phage structural modules, and they are very similar to those reported for coliphage p2 and p2-like phages. a phirsa1 minirepli ... | 2008 | 17965167 |
| induction of a small heat shock protein and its functional roles in nicotiana plants in the defense response against ralstonia solanacearum. | in tobacco (nicotiana tabacum), ralstonia solanacearum oe1-1 (rsoe1-1) is pathogenic, whereas r. solanacearum 8107 (rs8107) is nonpathogenic and induces the hypersensitive response (hr). to elucidate the molecular mechanisms of plant-r. solanacearum interactions, we used differential display to isolate a cdna fragment, a6, regulated in tobacco by inoculation with rsoe1-1. the deduced amino acid sequence predicted from full-length a6-cdna showed similarity to small heat shock proteins from arabid ... | 2007 | 17965181 |
| naphthalene metabolism and growth inhibition by naphthalene in polaromonas naphthalenivorans strain cj2. | this study was designed to characterize naphthalene metabolism in polaromonas naphthalenivorans cj2. comparisons were completed using two archetypal naphthalene-degrading bacteria: pseudomonas putida ncib 9816-4 and ralstonia sp. strain u2, representative of the catechol and gentisate pathways, respectively. strain cj2 carries naphthalene catabolic genes that are homologous to those in ralstonia sp. strain u2. here we show that strain cj2 metabolizes naphthalene via gentisate using respirometry, ... | 2007 | 17975081 |
| treatment of arsenic contaminated water in a batch reactor by using ralstonia eutropha mtcc 2487 and granular activated carbon. | this paper presents the observations on the bio-removal of arsenic from contaminated water by using ralstonia eutropha mtcc 2487 and activated carbon in a batch reactor. the effects of agitation time, ph, type of granular activated carbon (gac) and initial arsenic concentration (as(o)) on the % removal of arsenic have been discussed. under the experimental conditions, optimum removal was obtained at the ph of 6-7 with agitation time of 100 h. the % removal of as(t) increased initially with the i ... | 2008 | 17980486 |
| [analysis of signal peptides of the secreted proteins in ralstonia solanacearum gmi1000]. | the 3,440 open reading frames (orfs) of ralstonia solanacearum gmi1000 were used for predicting signal peptides by comprehensive analyses with signalp 3.0, tmhmm 2.0, targetp 1.01, lipop 1.0 and psortb, and screening based on l value. total 186 signal peptides with conserved amino acid residues are found and among them, 134 are secretary types, 22 are rr-motif types and 30 are lipoprotein signal peptides. the length distribution of signal peptide and its domains were analyzed systemically. no ty ... | 2007 | 17989054 |
| effects of enrichment with phthalate on polycyclic aromatic hydrocarbon biodegradation in contaminated soil. | the effect of enrichment with phthalate on the biodegradation of polycyclic aromatic hydrocarbons (pah) was tested with bioreactor-treated and untreated contaminated soil from a former manufactured gas plant (mgp) site. soil samples that had been treated in a bioreactor and enriched with phthalate mineralized (14)c-labeled phenanthrene and pyrene to a greater extent than unenriched samples over a 22.5-h incubation, but did not stimulate benzo[a]pyrene mineralization. in contrast to the positive ... | 2008 | 17990065 |
| ralstonia pickettii bloodstream infections at a brazilian cancer institution. | we describe a series of ralstonia pickettii bloodstream infections (bsi) that occurred in 19 oncohematologic patients admitted to a hospital for patients with cancer, in the city of rio de janeiro, from july 1999 to february 2006. fifty-four r. pickettii isolates were recovered from blood and catheter-tip specimens (1-5 isolates per patient). two patients eventually died of causes unrelated to r. pickettii bsi. eight pulsed-field gel electrophoresis genotypes were resolved (a-h), with two detect ... | 2008 | 17994262 |
| analyses of six homologous proteins of protochlamydia amoebophila uwe25 encoded by large gc-rich genes (lgr): a model of evolution and concatenation of leucine-rich repeats. | along the chromosome of the obligate intracellular bacteria protochlamydia amoebophila uwe25, we recently described a genomic island pam100g. it contains a tra unit likely involved in conjugative dna transfer and lgre, a 5.6-kb gene similar to five others of p. amoebophila: lgra to lgrd, lgrf. we describe here the structure, regulation and evolution of these proteins termed lgrs since encoded by "large g+c-rich" genes. | 2007 | 18021397 |
| combination of n149s and d171g mutations in aeromonas caviae polyhydroxyalkanoate synthase and impact on polyhydroxyalkanoate biosynthesis. | aeromonas caviae polyhydroxyalkanoate synthase (phac(ac)) is an important biocatalyst for the synthesis of practically useful two-component polyhydroxyalkanoate copolymer, poly[(r)-3-hydroxybutyrate-co-(r)-3-hydroxyhexanoate] [p(3hb-co-3hhx)]. in a previous study, two phac(ac) mutants that have a single amino acid substitution of either asparagine 149 by serine (n149s) or aspartate 171 by glycine (d171g) were isolated as higher active enzymes by means of evolutionary engineering. in this study, ... | 2007 | 18031343 |
| the geomicrobiology of gold. | microorganisms capable of actively solubilizing and precipitating gold appear to play a larger role in the biogeochemical cycling of gold than previously believed. recent research suggests that bacteria and archaea are involved in every step of the biogeochemical cycle of gold, from the formation of primary mineralization in hydrothermal and deep subsurface systems to its solubilization, dispersion and re-concentration as secondary gold under surface conditions. enzymatically catalysed precipita ... | 2007 | 18043665 |
| microbial diversity in pharmaceutical product recalls and environments. | identification of microbial contaminants in product recalls and environmental samples provides important information on the possible contamination sources and distribution of microbial species in pharmaceutical environments. analysis of fda product recall data for 134 non-sterile pharmaceutical products from 1998 to september 2006 demonstrated that 48% of recalls were due to contamination by either burkholderia cepacia, pseudomonas spp., or ralstonia picketti, while yeast and mold contamination ... | 2007 | 18047177 |
| proteomic analysis of the proteins regulated by hrpb from the plant pathogenic bacterium burkholderia glumae. | plant pathogenic bacteria transfer effector proteins into plant cells via the hypersensitive response and pathogenicity (hrp) type iii protein secretion system (t3ss) during infection. the genes encoding the hrp t3ss are expressed only under plant apoplast-mimicking conditions in an arac-type transcriptional activator hrpb-dependent manner. to identify the proteins controlled by hrpb in burkholderia glumae in vitro, we constitutively expressed hrpb and analyzed the proteins showing altered expre ... | 2008 | 18050277 |
| two bacterial entophytes eliciting both plant growth promotion and plant defense on pepper (capsicum annuum l.). | plant growth-promoting rhizobacteria (pgpr) have the potential to be used as microbial inoculants to reduce disease incidence and severity and to increase crop yield. some of the pgpr have been reported to be able to enter plant tissues and establish endophytic populations. here, we demonstrated an approach to screen bacterial endophytes that have the capacity to promote the growth of pepper seedlings and protect pepper plants against a bacterial pathogen. initially, out of 150 bacterial isolate ... | 2007 | 18051359 |
| [microbial diversity in scorpion intestine (buthus martensii karsch)]. | scorpion is an important officinal animal, and has a high nutritional value. in this study, the culture-independent and culture-dependent methods were used to investigate the microbial diversity in the scorpion's intestine. results based on culture-independent method showed the bacteria to be related to alpha, beta, gamma-proteobacteria. bacteria isolated by the culture-dependent method were high g + c, gram-positive bacteria. the genera enterobacter, serratia and ochrobactrum were detected by b ... | 2007 | 18062268 |
| mesaconyl-coenzyme a hydratase, a new enzyme of two central carbon metabolic pathways in bacteria. | the coenzyme a (coa)-activated c5-dicarboxylic acids mesaconyl-coa and beta-methylmalyl-coa play roles in two as yet not completely resolved central carbon metabolic pathways in bacteria. first, these compounds are intermediates in the 3-hydroxypropionate cycle for autotrophic co2 fixation in chloroflexus aurantiacus, a phototrophic green nonsulfur bacterium. second, mesaconyl-coa and beta-methylmalyl-coa are intermediates in the ethylmalonyl-coa pathway for acetate assimilation in various bacte ... | 2008 | 18065535 |
| enzymatic oxidation of h2 in atmospheric o2: the electrochemistry of energy generation from trace h2 by aerobic microorganisms. | | 2008 | 18088128 |
| pcr-based specific detection of ralstonia solanacearum by amplification of cytochrome c1 signal peptide sequences. | a polymerase chain reaction (pcr)-based method was developed to detect the dna of ralstonia solanacearum, the causal agent of bacterial wilt in various crop plants. one pair of primers (ralsf and ralsr), designed using cytochrome c1 signal peptide sequences specific to r. solanacearum, produced a pcr product of 932 bp from 13 isolates of r. solanacearum from several countries. the primer specificity was then tested using dna from 21 isolates of ralstonia, pseudomonas, burkholderia, xanthomonas, ... | 2007 | 18092459 |
| poly(3-hydroxybutyrate) (phb) depolymerase phaza1 is involved in mobilization of accumulated phb in ralstonia eutropha h16. | the recently finished genome sequence of ralstonia eutropha h16 harbors nine genes that are thought to encode functions for intracellular depolymerization (mobilization) of storage poly(3-hydroxybutyrate) (phb). based on amino acid similarities, the gene products belong to four classes (phaza1 to phaza5, phazb, phazc, and phazd1/phazd2). however, convincing direct evidence for the in vivo roles of the gene products is poor. in this study, we selected four candidate genes (phaza1, phazb, phazc, a ... | 2008 | 18156336 |
| identification of two aflp markers linked to bacterial wilt resistance in tomato and conversion to scar markers. | tomato bacterial wilt (bw) incited by ralstonia solanacearum is a constraint on tomato production in tropical, subtropical and humid regions of the world. in this paper, we present the results of a research aimed at the identification of pcr-based markers amplified fragment length polymorphism (aflp) linked to the genes that confer resistance to tomato bw. to this purpose, bulked segregant analysis was applied to an f(2) population segregating for the bw resistant gene and derived from the pair- ... | 2009 | 18157700 |
| the influence of process parameters on the characteristics of polyhydroxyalkanoates produced by mixed cultures. | the characterization of polyhydroxyalkanoates (pha) produced by mixed cultures is fundamental for foreseeing the possible final applications of the polymer. in this study pha produced under aerobic dynamic feeding (adf) conditions are characterized. the pha produced shows a stable average molecular weight ([symbol: see text]) in the range (1.0-3.0) x 10(6), along three years of reactor operation. attempts to improve the amount of pha produced did not introduce significant variations on the value ... | 2008 | 18157852 |
| modeling photosynthetically oxygenated biodegradation processes using artificial neural networks. | the complexity of the mechanisms underlying organic matter mineralization and nutrient removal in algal-bacterial photobioreactors during the treatment of residual wastewaters has severely hindered the development of mechanistic models able to accurately describe these processes. artificial neural networks (anns) are capable of inferring the complex relationships existing between input and output process variables without a detailed description of the mechanisms governing the process, and should ... | 2008 | 18164545 |
| production of poly(3-hydroxybutyrate) from waste potato starch. | there has been a considerable interest in using low cost carbon substrates for the production of poly(3-hydroxybutyrate) (phb). we have shown that saccharified waste potato starch can be used as a viable alternative carbon source in high cell density phb production. using ralstonia eutropha ncimb 11599 with phosphate limitation, 179 g/l biomass, 94 g/l phb, y(biomass/starch) = 0.46 g/g, y(phb/starch) = 0.22 g/g, and phb productivity = 1.47 g/(l*h) were achieved. residual maltose accumulated in t ... | 2008 | 18175895 |
| streptomyces griseus enhances denitrification by ralstonia pickettii k50, which is possibly mediated by histidine produced during co-culture. | ralstonia pickettii k50 (strain k50) is a denitrifying bacterium that produces low levels of n(2)o under aerobic conditions. in this study, we found that co-culturing of strain k50 with streptomyces griseus significantly enhanced the denitrification activity of strain k50 in an artificial wastewater (aww) system. most factors that enhance denitrification activity were in the high molecular weight fraction of the cell-free broth of s. griseus, and were suggested to be extracellular proteases. fur ... | 2008 | 18175905 |
| transcription dynamics of the functional tfda gene during mcpa herbicide degradation by cupriavidus necator aeo106 (pro101) in agricultural soil. | a modified protocol for simultaneous extraction of rna and dna, followed by real-time polymerase chain reaction quantification, was used to investigate tfda gene expression during in situ degradation of the herbicide mcpa (4-chloro-2-methylphenoxy-acetic acid) in soil. tfda encodes an alpha-ketoglutarate-dependent dioxygenase catalysing the first step in the degradation pathway of mcpa and 2,4-d (2,4-dichlorophenoxy-acetic acid). a linear recovery of tfda mrna over three orders of magnitude was ... | 2008 | 18190516 |
| structural basis for mannose recognition by a lectin from opportunistic bacteria burkholderia cenocepacia. | chronic colonization of the lungs by opportunist bacteria such as pseudomonas aeruginosa and members of the bcc (burkholderia cepacia complex) is the major cause of morbidity and mortality among cf (cystic fibrosis) patients. pa-iil (lecb gene), a soluble lectin from ps. aeruginosa, has been the subject of much interest because of its very strong affinity for fucose. orthologues have been identified in the opportunist bacteria ralstonia solanacearum, chromobacterium violaceum and burkholderia of ... | 2008 | 18215132 |
| monitoring of phytopathogenic ralstonia solanacearum cells using green fluorescent protein-expressing plasmid derived from bacteriophage phirss1. | a green fluorescent protein (gfp)-expressing plasmid was constructed from a filamentous bacteriophage phirss1 that infects the phytopathogen ralstonia solanacearum. this plasmid designated as prss12 (4.7 kbp in size) consists of an approximately 2248 bp region of the phirss1 rf dna, including orf1-orf3 and the intergenic region (ig), and a km cassette in addition to the gfp gene. it was easily introduced by electroporation and stably maintained even without selective pressure in strains of r. so ... | 2007 | 18215630 |
| improvement of poly(3-hydroxybutyrate) [p(3hb)] production in corynebacterium glutamicum by codon optimization, point mutation and gene dosage of p(3hb) biosynthetic genes. | in our previous study, a system for producing poly(3-hydroxybutyrate) [p(3hb)] was established by introducing a polyhydroxyalkanoate (pha) biosynthetic gene operon (phacab re) derived from ralstonia eutropha into corynebacterium glutamicum. in this study, two experimental strategies have been applied to improve p(3hb) production in recombinant c. glutamicum. one is a codon optimization of the n-terminal-coding region of the pha synthase (phac re) gene focusing on the codon usage preference for t ... | 2007 | 18215631 |
| burkholderia cepacia complex bacteria: opportunistic pathogens with important natural biology. | interaction with plants around their roots and foliage forms the natural habitat for a wide range of gram-negative bacteria such as burkholderia, pseudomonas and ralstonia. during these interactions many of these bacteria facilitate highly beneficial processes such as the breakdown of pollutants or enhancement of crop growth. all these bacterial species are also capable of causing opportunistic infections in vulnerable individuals, especially people with cystic fibrosis (cf). here we will review ... | 2008 | 18217926 |
| binding of the major phasin, phap1, from ralstonia eutropha h16 to poly(3-hydroxybutyrate) granules. | the surface of polyhydroxybutyrate (phb) storage granules in bacteria is covered mainly by proteins referred to as phasins. the layer of phasins stabilizes the granules and prevents coalescence of separated granules in the cytoplasm and nonspecific binding of other proteins to the hydrophobic surfaces of the granules. phasin phap1(reu) is the major surface protein of phb granules in ralstonia eutropha h16 and occurs along with three homologues (phap2, phap3, and phap4) that have the capacity to ... | 2008 | 18223073 |
| characterization and expression of an nsltps-like antimicrobial protein gene from motherwort (leonurus japonicus). | in screening for potent antimicrobial proteins (amps) from plant seeds, we had purified a heat-stable amp, ljamp2, from the seeds of a medicine herb, motherwort (leonurus japonicus houtt). in an in vitro assay, the protein can inhibit the growth of both fungi and bacteria. then a cdna encoding ljamp2 was cloned by the rapid amplification of cdna ends based on the n-terminal amino acid sequence determined. the deduced amino acid sequences of this cdna show similarity to plant non-specific lipid t ... | 2008 | 18228022 |
| selective production of epothilone b by heterologous expression of propionyl-coa synthetase in sorangium cellulosum. | the metabolic engineering of epothilones, as secondary metabolites, was investigated using sorangium cellulosum to achieve the selective production of epothilone b, a potent anticancer agent. thus, the propionyl-coa synthetase gene (prpe) from ralstonia solanacearum was heterologously expressed in s. cellulosum to increase the production of epothilone b. propionyl-coa synthetase converts propionate into propionyl-coa, a potent precursor of epothilone b. the recombinant s. cellulosum containing t ... | 2008 | 18239430 |
| evidence of aerobic utilization of di-ortho-substituted trichlorobiphenyls as growth substrates by pseudomonas sp. sa-6 and ralstonia sp. sa-4. | robust and effective bioremediation strategies have not yet been developed for polychlorinated biphenyl (pcb)-contaminated soils. this is in part a result of the fact that ortho- or ortho- and para-substituted congeners, frequent dead-end products of reductive dechlorination of pcb mixtures, have greatly reduced aerobic biodegradability. in this study, we report substantial evidence of utilization of diortho-substituted trichlorobiphenyls (tricbs) as growth substrates by ralstonia sp. sa-4 and p ... | 2008 | 18248454 |
| abscisic acid regulates tsrf1-mediated resistance to ralstonia solanacearum by modifying the expression of gcc box-containing genes in tobacco. | although recent studies have established a significant regulatory role for abscisic acid (aba) and ethylene response factor (erf) proteins in plant pathogen resistance, it is not clear whether and how aba performs this role. previously, it was reported that an erf protein, tsrf1, activates the expression of gcc box-containing genes and significantly enhances the resistance to ralstonia solanacearum in both tobacco and tomato plants. here, it is reported that tsrf1-regulated pathogen resistance i ... | 2008 | 18252700 |
| 16s rdna analysis reveals low microbial diversity in community level physiological profile assays. | the metabolic diversity of microbial communities is fundamental for the multiple soil functions mediated by microorganisms. community level physiological profiles (clpps) based on sole c source oxidation have been used as a fast and reproducible tool to study soil microbial functional diversity because the utilisation of available carbon is the key factor governing microbial growth in soil. our aim was to assess the phylogenetic affiliation of the microorganisms responsible for c consumption aft ... | 2008 | 18258321 |
| novel acetylated alpha-cyclosophorotridecaose produced by ralstonia solanacearum. | alpha-cyclosophorotridecaose (alpha-c13) produced by ralstonia solanacearum is isolated by trichloroacetic acid treatment and subjected to various chromatographic techniques. here, we report for the first time that r. solanacearum produces acetylated alpha-c13. structural analyses of the acetylated alpha-c13 were performed with 1d or 2d nmr spectroscopy, maldi-tof ms and hplc. the results show that the alpha-c13 is substituted by mainly one acetyl residue at the c-6 position of the glucose unit. | 2008 | 18262507 |
| neonatal sepsis caused by ralstonia pickettii. | | 2008 | 18277913 |
| comparative phylogenies of burkholderia, ralstonia, comamonas, brevundimonas and related organisms derived from rpob, gyrb and rrs gene sequences. | phylogenetic analysis of strains from burkholderia, ralstonia, cupriavidus, comamonas, delftia, acidovorax, brevundimonas, herbaspirillum huttiense and "pseudomonas butanovora" was performed based on the protein-coding genes rpob and gyrb and on the 16s rrna-coding gene rrs. overall, the phylogenies deduced from the three genes were concordant among themselves and with current taxonomy. however, a few differences among individual gene phylogenies were noted. for example, the separation of cupria ... | 2008 | 18280706 |
| phylogenetic analysis of long-chain hydrocarbon-degrading bacteria and evaluation of their hydrocarbon-degradation by the 2,6-dcpip assay. | thirty-six bacteria that degraded long-chain hydrocarbons were isolated from natural environments using long-chain hydrocarbons (waste car engine oil, base oil or the c-alkane fraction of base oil) as the sole carbon and energy source. a phylogenetic tree of the isolates constructed using their 16s rdna sequences revealed that the isolates were divided into six genera plus one family (acinetobacter, rhodococcus, gordonia, pseudomonas, ralstonia, bacillus and alcaligenaceae, respectively). furthe ... | 2008 | 18283542 |
| origin and evolution of gala-lrr, a new member of the cc-lrr subfamily: from plants to bacteria? | the phytopathogenic bacterium ralstonia solanacearum encodes type iii effectors, called gala proteins, which contain f-box and lrr domains. the gala lrrs do not perfectly fit any of the previously described lrr subfamilies. by applying protein sequence analysis and structural prediction, we clarify this ambiguous case of lrr classification and assign gala-lrrs to cc-lrr subfamily. we demonstrate that side-by-side packing of lrrs in the 3d structures may control the limits of repeat variability w ... | 2008 | 18301771 |
| crystalline-structure-dependent enzymatic degradation of polymorphic poly(3-hydroxypropionate). | the crystalline structure dependence of enzymatic degradation behavior was investigated for the polymorphic poly(3-hydroxypropionate) (p3hp), which has a basic backbone chemical structure of bacterial poly(3-hydroxyalkanoate)s (p3has). the p3hp films consisting of the beta-, gamma-, and/or delta-form crystal were cast or melt-crystallized as reported previously (macromolecules 2005, 38, 6455; macromolecules 2006, 39, 194-203) by controlling the molecular weight, crystallization temperature, and/ ... | 2008 | 18307304 |
| real-time pcr quantification of pah-ring hydroxylating dioxygenase (pah-rhdalpha) genes from gram positive and gram negative bacteria in soil and sediment samples. | real-time pcr based assays were developed to quantify gram positive (gp) and gram negative (gn) bacterial populations that are capable of degrading the polycyclic aromatic hydrocarbons (pah) in soil and sediment samples with contrasting contamination levels. these specific and sensitive real-time pcr assays were based on the quantification of the copy number of the gene that encodes the alpha subunit of the pah-ring hydroxylating dioxygenases (pah-rhdalpha), involved in the initial step of the a ... | 2008 | 18329116 |
| [screening, identification, and antagonism assessment, of dominant bacteria in ageratina adenophora sprengel rhizosphere soil]. | by using isolation and culture method, 25 strains of dominant bacteria in ageratina adenophora rhizosphere soil were isolated and identified, of which, 8 strains were assessed for their antagonistic activity. the results showed that bacillus and pseudomonas were highly abundant in a. adenophora rhizosphere soil, of which, b. subtilis and b. megaterium were most abundant and occupied 55.6% of the total identified bacteria. these dominant bacteria had different level antagonistic activity to fusar ... | 2007 | 18333457 |
| removal of heavy metals by an enriched consortium. | an enriched consortium obtained from lake-sediment was developed for the removal of heavy metals such as cu, pb, cr, ni, and zn from heavy metal-contaminated water. the removal efficiency of heavy metals in a shaking condition was generally higher than that in the static state. after the fifteenth enrichment with assorted heavy metals, the removal efficiencies in the shaking and static condition at an average concentration of 100 mg/l of each heavy metal were approximately 99 approximately 100% ... | 2008 | 18337688 |
| secretion pathway for the poly(3-hydroxybutyrate) depolymerase in ralstonia pickettii t1. | the extracellular poly(3-hydroxybutyrate) depolymerase from ralstonia pickettii t1 has been purified, its function and character investigated in detail, and its gene cloned and sequenced. however, the mechanism by which this enzyme is secreted has not been elucidated. a mutant unable to degrade poly(3-hydroxybutyrate), n17, was obtained with the random insertion of a mini-transposon, tn5. western analysis using antiserum against the poly(3-hydroxybutyrate) depolymerase of ralstonia pickettii t1, ... | 2008 | 18340545 |
| quantifying the surface characteristics and flocculability of ralstonia eutropha. | the microbial surface and flocculability were qualitatively characterized through the combination of the surface thermodynamic and the extended dlvo approaches, with ralstonia eutropha, a polyhydroxybutyrate-producing bacterium, as an example. the negativity of the zeta potential of r. eutropha decreased from the initial -19.5 to -11 mv in its cultivation with the consumption of glucose. the total interfacial free energy (deltagadh) was changed from -80 to 28.5 mj m(-2) in its entire growth proc ... | 2008 | 18347792 |
| effects of plant genotype and growth stage on the structure of bacterial communities associated with potato (solanum tuberosum l.). | the effects of genotype, plant growth and experimental factors (soil and year) on potato-associated bacterial communities were studied. cultivars achirana inta, désirée, merkur and transgenic désirée line dl12 (containing t4 lysozyme gene) were assessed in two field experiments. cross-comparisons between both experiments were made using désirée plants. culture-dependent and -independent approaches were used to demonstrate effects on total bacterial, actinobacterial and pseudomonas communities in ... | 2008 | 18355298 |
| response coefficient analysis of a fed-batch bioreactor to dissolved oxygen perturbation in complementary cultures during phb production. | abstract: | 2008 | 18366808 |
| thermally treated grass fibers as colonizable substrate for beneficial bacterial inoculum. | this study investigates how thermally treated (i.e., torrefied) grass, a new prospective ingredient of potting soils, is colonized by microorganisms. torrefied grass fibers (tgf) represent a specific colonizable niche, which is potentially useful to establish a beneficial microbial community that improves plant growth. tgf and torrefied grass extracts (tge) were inoculated with a suspension of microorganisms obtained from soil. sequential microbial enrichment steps were then performed in both su ... | 2008 | 18368438 |
| two xanthomonas extracellular polygalacturonases, pghaxc and pghbxc, are regulated by type iii secretion regulators hrpx and hrpg and are required for virulence. | xanthomonas campestris pv. campestris, the causal agent of black rot disease, produces a suite of extracellular cell-wall degrading enzymes (cwde) that are involved in bacterial virulence. polygalacturonase (pg) is an important cwde and functions to degrade the pectic layers of plant cell walls. although previous studies have documented the virulence functions of pg in erwinia and ralstonia species, the regulation of pg genes still needs to be elucidated. in this study, we identified two novel p ... | 2008 | 18393615 |
| uv mutagenesis of cupriavidus necator for extracellular production of (r)-3-hydroxybutyric acid. | ultraviolet (uv) mutagenesis was carried out to obtain mutant strains of cupriavidus necator that could produce (r)-3-hydroxybutyric acid [(r)-3-hb] in the culture supernatant. | 2008 | 18399978 |
| enhancement of bioremediation by ralstonia sp. hm-1 in sediment polluted by cd and zn. | in this study, the potential for the application of the bioaugmentation to cd and zn contaminated sediment was investigated. a batch experiment was performed in the lake sediments augmented with ralstonia sp. hm-1. the degradation capacity of 18.7 mg-doc/l/day in the treatment group was bigger than that of the blank group (4.4 mg-doc/l/day). it can be regarded as the result of the reduction of the metal concentration in the liquid phase due to adsorption into the sediments, with the increased al ... | 2008 | 18417340 |
| prokaryotic diversity in one of the largest hypersaline coastal lagoons in the world. | araruama lagoon is an environment characterized by high salt concentrations. the low raining and high evaporation rates in this region favored the development of many salty ponds around the lagoon. in order to reveal the microbial composition of this system, we performed a 16s rrna gene survey. among archaea, most clones were related to uncultured environmental euryarchaeota. in lagoon water, we found some clones related to methanomicrobia and methanothermococcus groups, while in the saline pond ... | 2008 | 18427718 |
| redox-controlled dinitrosyl formation at the diiron-oxo center of nora. | in the denitrifying bacterium ralstonia eutropha h16, the nora protein is coproduced with the respiratory nitric oxide (no) reductase. nora contains a diiron-oxo center, which can form stable adducts with dioxygen and no. in contrast to other diiron proteins, the formation of nora-no requires both fully reduced protein and additional electrons. a minor fraction of in vitro nora-no represents a paramagnetic dinitrosyl iron complex (dnic), while the major fraction is attributed to a dnic of the st ... | 2008 | 18433625 |
| dipeptide synthesis by l-amino acid ligase from ralstonia solanacearum. | despite its utility, dipeptides have not been widely used due to the absence of an efficient manufacturing method. recently, a novel method for effective production of dipeptides using l-amino acid alpha-ligase (lal) is presented. lal, which is only identified in bacillus subtilis, catalyzes dipeptide synthesis from unprotected amino acids in an atp-dependent manner. however, not all the dipeptide can be synthesized by lal from b. subtilis (bslal) due to its substrate specificity. here, we attem ... | 2008 | 18445480 |