| microbiology of ventilator-associated pneumonia compared with that of hospital-acquired pneumonia. | nosocomial pneumonia is the leading cause of mortality attributed to nosocomial infection. appropriate empirical therapy has been associated with improved survival, but data are limited regarding the etiologic agents of hospital-acquired pneumonia in nonventilated patients (hap). this evaluation assessed whether the currently recommended empirical therapy is appropriate for both ventilator-associated pneumonia (vap) and hap by evaluating the infecting flora. | 2007 | 17564985 |
| fatal pulmonary hemorrhage in a patient with myelodysplastic syndrome and fulminant pneumonia caused by stenotrophomonas maltophilia. | | 2007 | 17565466 |
| effect of environmental factors on biofilm formation by clinical stenotrophomonas maltophilia isolates. | the influence of environmental factors (temperature, aerobiosis-anaerobiosis, static-dynamic conditions, ph) was determined on biofilm formation by 51 s. maltophilia clinical isolates. the strains produced more biofilm at 32 degrees c than at 37 or 18 degrees c. aerobic and 6% co2 atmosphere yielded comparable biofilm amounts, higher than under anaerobic conditions. biofilm production was not affected by static vs. agitated culture conditions. biofilm production at ph 7.5 and 8.5 was comparable ... | 2007 | 17571802 |
| successful engraftment of the second reduced-intensity conditioning cord blood transplantation (cbt) for a patient who developed graft rejection and infectious complications after the first cbt for aml. | | 2007 | 17572713 |
| a cyclic amp receptor protein-regulated cell-cell communication system mediates expression of a feca homologue in stenotrophomonas maltophilia. | stenotrophomonas maltophilia wr-c possesses an rpf/diffusible signal factor (dsf) cell-cell communication system. it produces cis-delta2-11-methyl-dodecenoic acid, a dsf, and seven structural derivatives, which require rpff and rpfb for synthesis. acquisition of iron from the environment is important for bacterial growth as well as the expression of virulence genes. we identified a gene homologous to feca, which encodes a ferric citrate receptor that transports exogenous siderophore ferric citra ... | 2007 | 17574998 |
| isotopic analysis of n and o in nitrite and nitrate by sequential selective bacterial reduction to n2o. | nitrite is an important intermediate species in the biogeochemical cycling of nitrogen, but its role in natural aquatic systems is poorly understood. isotopic data can be used to study the sources and transformations of no2- in the environment, but methods for independent isotopic analyses of no2- in the presence of other n species are still new and evolving. this study demonstrates that isotopic analyses of n and o in no2- can be done by treating whole freshwater or saltwater samples with the d ... | 2007 | 17580983 |
| [clinical distribution and antibiotic resistance of non-fermentative gram-negative bacilli infection after liver transplantation]. | to investigate the epidemiology of non-fermentative gram-negative bacilli infections after liver transplantation, and to analyze the antibiotic susceptibility to major pathogens. | 2007 | 17593853 |
| atrazine degradation by aerobic microorganisms isolated from the rhizosphere of sweet flag (acorus calamus l.). | in presented study the capability of microorganisms isolated from the rhizosphere of sweet flag (acorus calamus) to the atrazine degradation was assessed. following isolation of the microorganisms counts of psychrophilic bacteria, mesophilic bacteria and fungi were determined. isolated microorganisms were screened in terms of their ability to decompose a triazine herbicide, atrazine. our results demonstrate that within the rhizosphere of sweet flag there were 3.8x10(7) cfu of psychrophilic bacte ... | 2008 | 17594524 |
| convenient test using a combination of chelating agents for detection of metallo-beta-lactamases in the clinical laboratory. | although transmissible metallo-beta-lactamases (mbls) are a serious threat to beta-lactam antibiotic therapy, the clsi currently does not recommend testing methods for the detection of mbls. the aim of this study was to evaluate the capability of double-disk tests (ddts) by using disks containing a combination of the chelators 2-mercaptopropionic acid (mpa) and tris-edta (te) to detect mbls. sixteen isolates (4 acinetobacter baumannii isolates, 6 pseudomonas aeruginosa isolates, 1 serratia marce ... | 2007 | 17596358 |
| rhizobacterial volatiles affect the growth of fungi and arabidopsis thaliana. | volatiles of stenotrophomonas, serratia, and bacillus species inhibited mycelial growth of many fungi and arabidopsis thaliana (40 to 98%), and volatiles of pseudomonas species and burkholderia cepacia retarded the growth to lesser extents. aspergillus niger and fusarium species were resistant, and b. cepacia and staphylococcus epidermidis promoted the growth of rhizoctonia solani and a. thaliana. bacterial volatiles provide a new source of compounds with antibiotic and growth-promoting features ... | 2007 | 17601806 |
| cultivable bacterial diversity of alkaline lonar lake, india. | aerobic, alkaliphilic bacteria were isolated and characterized from water and sediment samples collected in the winter season, january 2002 from alkaline lonar lake, india, having ph 10.5. the total number of microorganisms in the sediment and water samples was found to be 10(2)-10(6) cfu g(-1) and 10(2)-10(4) cfu ml(-1), respectively. one hundred and ninety-six strains were isolated using different enrichment media. to study the bacterial diversity of lonar lake and to select the bacterial stra ... | 2008 | 17604989 |
| high-throughput determination of biochemical oxygen demand (bod) by a microplate-based biosensor. | the use of the conventional 5-day biochemical oxygen demand (bod5) method in bod determination is greatly hampered by its time-consuming sampling procedure and its technical difficulty in the handling of a large pool of wastewater samples. thus, it is highly desirable to develop a fast and high-throughput biosensor for bod measurements. this paper describes the construction of a microplate-based biosensor consisting of an organically modified silica (ormosil) oxygen sensing film for high-through ... | 2007 | 17612187 |
| ventilator-associated pneumonia in the home care setting. | to describe the rate of infection, associated organisms, and potential risk factors for ventilator-associated pneumonia (vap) in patients receiving mechanical ventilation at home. | 2007 | 17620236 |
| [the present status, counter-measures and new trends on burn infection]. | in recent fifty years, pseudomonas aeruginosa and staphylococcus aureus were continuously the predominant in burn infections, the only change seen was a rapid increase in their drug-resistance. under the pressure of antibiotics, some opportunistic bacteria that were resistant to all available antibiotics emerged, such as acinetobacter baumanii and maltophilia stenotrophomonas. for critically burn patients, basing on early surgical intervention, early and short-term use of broad-spectrum antibiot ... | 2007 | 17649876 |
| bacterial contamination of water in dental unit reservoirs. | the aim of this study was bacteriological assessment of water in dental unit reservoirs--concentration and composition of the aerobe and facultative anaerobe bacterial microflora. reservoir water samples were taken from 25 units. bacterial flora were determined with the plate culture method. bacteria were identified with biochemical microtests: api 20e, api 20ne (biomérieux, france) and gp2 microplatetm (biolog, usa). the concentration of total bacteria isolated from one site was 201,039 cfu/ml, ... | 2007 | 17655191 |
| in vitro efficacy of copper and silver ions in eradicating pseudomonas aeruginosa, stenotrophomonas maltophilia and acinetobacter baumannii: implications for on-site disinfection for hospital infection control. | pseudomonas aeruginosa, stenotrophomonas maltophilia and acinetobacter baumannii are major opportunistic waterborne pathogens causing hospital-acquired infections. copper-silver ionization has been shown to be effective in controlling legionella colonization in hospital water systems. the objective was to determine the efficacy of copper and silver ions alone and in combination in eradicating p. aeruginosa, s. maltophilia and a. baumannii at the concentration applied to legionella control. kill ... | 2008 | 17655912 |
| life-threatening septic shock in a pregnant woman with ileus. | septic shock during pregnancy is very rare but has high mortality. we report a case of septic shock in a pregnant woman with ileus, showing that severe ileus in a pregnant woman could be attributed to life-threatening septic shock and that we should give special attention to a nosocomial infection. | 2007 | 17674261 |
| isolation of maize soil and rhizosphere bacteria with antagonistic activity against aspergillus flavus and fusarium verticillioides. | bacterial isolates from mississippi maize field soil and maize rhizosphere samples were evaluated for their potential as biological control agents against aspergillus flavus and fusarium verticillioides. isolated strains were screened for antagonistic activities in liquid coculture against a. flavus and on agar media against a. flavus and f. verticillioides. we identified 221 strains that inhibited growth of both fungi. these bacteria were further differentiated by their production of extracellu ... | 2007 | 17685333 |
| endophytic bacterial diversity in rice (oryza sativa l.) roots estimated by 16s rdna sequence analysis. | the endophytic bacterial diversity in the roots of rice (oryza sativa l.) growing in the agricultural experimental station in hebei province, china was analyzed by 16s rdna cloning, amplified ribosomal dna restriction analysis (ardra), and sequence homology comparison. to effectively exclude the interference of chloroplast dna and mitochondrial dna of rice, a pair of bacterial pcr primers (799f-1492r) was selected to specifically amplify bacterial 16s rdna sequences directly from rice root tissu ... | 2008 | 17690836 |
| antibiotic deactivation by a dizinc beta-lactamase: mechanistic insights from qm/mm and dft studies. | hybrid quantum mechanical/molecular mechanical (qm/mm) methods and density functional theory (dft) were used to investigate the initial ring-opening step in the hydrolysis of moxalactam catalyzed by the dizinc l1 beta-lactamase from stenotrophomonas maltophilia. anchored at the enzyme active site via direct metal binding as suggested by a recent x-ray structure of an enzyme-product complex (spencer, j.; et al. j. am. chem. soc. 2005, 127, 14439), the substrate is well aligned with the nucleophil ... | 2007 | 17691780 |
| survival of lung transplant patients with cystic fibrosis harboring panresistant bacteria other than burkholderia cepacia, compared with patients harboring sensitive bacteria. | the impact of panresistant bacteria, other than burkholderia cepacia, on the survival after lung transplantation in patients with cystic fibrosis (cf) remains controversial. | 2007 | 17692788 |
| prevalence and antimicrobial susceptibility of microorganisms isolated from sputa of patients with cystic fibrosis. | new emerging pathogens and associated antimicrobial resistance mechanisms have been observed in the respiratory tract of patients suffering from cystic fibrosis (cf) in the last years. amongst others, the rate of metallo-beta-lactamase (mbl)-producing pseudomonas aeruginosa strains is growing. however, there are no published data on the prevalence of mbl-producing p. aeruginosa in cf patients to our knowledge. | 2008 | 17693140 |
| community structure analysis of reverse osmosis membrane biofilms and the significance of rhizobiales bacteria in biofouling. | the biofilm community structure of a biofouled reverse osmosis (ro) membrane was examined using a polyphasic approach, and the dominant phylotypes retrieved were related to the order rhizobiales, a group of bacteria that is hitherto not implicated in membrane biofouling. a comparison with two other membrane biofilms using t-rflp fingerprinting also revealed the dominance of rhizobiales organisms. when pure culture ro biofilm isolates were cultivated aerobically in biolog microplates, most rhizob ... | 2007 | 17695921 |
| antimicrobial resistance among gram-negative bacilli causing infections in intensive care unit patients in the united states between 1993 and 2004. | during the 12-year period from 1993 to 2004, antimicrobial susceptibility profiles of 74,394 gram-negative bacillus isolates recovered from intensive care unit (icu) patients in united states hospitals were determined by participating hospitals and collected in a central location. mics for 12 different agents were determined using a standardized broth microdilution method. the 11 organisms most frequently isolated were pseudomonas aeruginosa (22.2%), escherichia coli (18.8%), klebsiella pneumoni ... | 2007 | 17715376 |
| structural basis for the role of asp-120 in metallo-beta-lactamases. | metallo-beta-lactamases (mbetals) are zinc-dependent enzymes that hydrolyze a wide range of beta-lactam antibiotics. the mbetal active site features an invariant asp-120 that ligates one of the two metal ions (zn2) and a metal-bridging water/hydroxide (wat1). previous studies show that substitutions at asp-120 dramatically affect mbetal activity, but no consensus exists as to its role in beta-lactam turnover. here we present crystal structures of the asn and cys mutants of asp-120 of the l1 mbet ... | 2007 | 17715946 |
| antimicrobial properties of moderately halotolerant bacteria from cenotes of the yucatan peninsula. | isolation and antimicrobial evaluation of aquatic bacterial strains from two cenotes. | 2007 | 17718841 |
| stenotrophomonas terrae sp. nov. and stenotrophomonas humi sp. nov., two nitrate-reducing bacteria isolated from soil. | three gram-negative, rod-shaped, non-spore-forming, nitrate-reducing isolates (r-32746, r-32768(t) and r-32729(t)) were obtained from soil. analysis of repetitive sequence-based pcr showed that the three isolates represented two different strains. 16s rrna gene sequence analysis and dna-dna hybridization placed them within the genus stenotrophomonas and revealed that they were genotypically different from each other and from all recognized stenotrophomonas species. analysis of the fatty acid com ... | 2007 | 17766871 |
| biodegradation of fuel oil hydrocarbons by a mixed bacterial consortium in sandy and loamy soils. | the aerobic degradation of light fuel oil in sandy and loamy soils by an environmental bacterial consortium was investigated. soils were spiked with 1 or 0.1% of oil per dry weight of soil. acetone extracts of dried soils were analyzed by gc and the overall degradation was calculated by comparison with hydrocarbon recovery from uninoculated soils. in sandy soils, the sum of alkanes n-c(12) to n-c(23) was degraded to about 45% within 6 days at 20 degrees c and to 27-31% within 28 days, provided t ... | 2007 | 17806098 |
| spondylodiscitis due to stenotrophomonas maltophilia. | stenotrophomonas maltophilia is an increasingly recognized cause of severe nosocomial infections, especially in immunocompromised patients. community-acquired infections have also been reported. spondylodiscitis due to s. maltophilia has only once before been described in the literature. we present a case of spondylodiscitis due to community-acquired s. maltophilia infection in a renal transplant recipient with liver cirrhosis. | 2007 | 17822663 |
| stenotrophomonas maltophilia seite02, a new bacterial strain suitable for bioremediation of selenite-contaminated environmental matrices. | biochemical and proteomic tools have been utilized for investigating the mechanism of action of a new stenotrophomonas maltophilia strain (seite02), a gammaproteobacterium capable of resistance to high concentrations of selenite [seo(3)(2-), se(iv)], reducing it to nontoxic elemental selenium under aerobic conditions; this strain was previously isolated from a selenite-contaminated mining soil. biochemical analysis demonstrated that (i) nitrite reductase does not seem to take part in the process ... | 2007 | 17827320 |
| diversity of microorganisms in decaying maize stalks revealed by a molecular method. | microbial diversity in decaying maize stalk was characterized by constructing and analyzing rrna gene clone library. total 47 otus were obtained from 82 bacterial clones, including proteobacteria (64.6%), actinobacteria (30.5%), bacteroidetes (2.4%) and firmicutes (2.4%). most proteobacterial clones were members of rhizobium, pseudomonas and stenotrophomonas. eighty-four percent of actinobacteria was related to microbacterium. only 14 otus were identified from 124 fungal clones, including ascomy ... | 2007 | 17846593 |
| inhibition of stenotrophomonas maltophilia dihydrofolate reductase by methotrexate: a single slow-binding process. | although antifolates such as trimethoprim are used in the clinical treatment of stenotrophomonas maltophilia infection, the dihydrofolate reductase (dhfr) of this microorganism is scarcely known because it has never been isolated. here, we describe the purification of this enzyme and kinetically characterize its inhibition by methotrexate (mtx). upon mtx treatment, time-dependent, slow-binding inhibition was observed due to the generation of a long-lived, slowly dissociating enzyme-nadph-inhibit ... | 2007 | 17847703 |
| conjugative transfer of the incp-7 carbazole degradative plasmid, pcar1, in river water samples. | the transfer of the incp-7 carbazole degradative plasmid pcar1 from pseudomonas putida sm1443 (derived from strain kt2440) into bacteria of river water samples was monitored using a reporter gene encoding red fluorescent protein (rfp). the number of transconjugants drastically increased in the presence of carbazole, and most appeared to belong to the genus pseudomonas. the results suggest that the presence of carbazole benefits the appearance of transconjugants belonging to the genus pseudomonas ... | 2008 | 17851640 |
| molecular characterization of virulence determinants of stenotrophomonas maltophilia strains isolated from patients affected by cystic fibrosis. | stenotrophomonas maltophilia is an emerging nosocomial bacterial pathogen which is currently isolated with increasing frequency from the airways of cystic fibrosis (cf) patients. in this study 13 s. maltophilia strains (11 isolated from the airways of independent cf patients, and two non-cf respiratory reference strains) have been characterized for the expression of several virulence-associated factors. in particular, the ability to form biofilm on abiotic surfaces has been determined and correl ... | 2007 | 17880766 |
| cutaneous infections with stenotrophomonas maltophilia in patients using immunosuppressive medication. | | 2007 | 17894750 |
| molecular phylogenetic diversity of bacteria and its spatial distribution in composts. | to investigate microbial diversity of swine manure composts in the initial stage and the spatial distribution due to gradient effect. | 2007 | 17897238 |
| sensitivity pattern of gram negative bacilli to three beta-lactam/beta-lactamase inhibitor combinations using the automated api system. | to evaluate the spectrum of activity of three beta-lactamase inhibitors such as amoxicillin/ clavulanic acid, ticarcillin/ clavulanic acid and piperacillin/ tazobactam in comparison to cephalosporins against gram negative bacilli. | 2007 | 17901635 |
| extensively drug-resistant stenotrophomonas maltophilia in a tertiary care hospital in taiwan: microbiologic characteristics, clinical features, and outcomes. | a retrospective study was performed in a university-affiliated referral center from year 1999 to year 2004 to understand the trend of resistance in stenotrophomonas maltophilia isolates associated with nosocomial infections and describe the emergence of an extensively drug-resistant s. maltophilia (xdrsm). a 49% increase in all types of nosocomial infections and an 85% increase in nosocomial bloodstream infections caused by s. maltophilia were noted over the 5-year period. trends of resistance t ... | 2008 | 17950557 |
| comparative analysis of the bactericidal activities of amphibian peptide analogues against multidrug-resistant nosocomial bacterial strains. | due to the widespread resistance of bacteria to the available drugs, the discovery of new classes of antibiotics is urgently needed, and naturally occurring antimicrobial peptides (amps) are considered promising candidates for future therapeutic use. amphibian skin is one of the richest sources of such amps. in the present study we compared the in vitro bactericidal activities of five amps from three different species of anurans against multidrug-resistant clinical isolates belonging to species ... | 2008 | 17954700 |
| inhibitory effect of gram-negative and gram-positive microorganisms against helicobacter pylori clinical isolates. | to determine the in vitro inhibitory effect of several gram-negative and gram-positive microorganisms against helicobacter pylori clinical isolates. | 2008 | 17965421 |
| [significance of the sampling points and the aerobic microbiological culture for the diagnosis of respiratory infections in reptiles]. | respiratory diseases play an important role in reptiles kept in captivity. microbiological examinations are described as an essential part of the diagnostic possibilities. therefore the aim of this study was to collect data on the usefulness of results obtained after aerobic culture (sheepblood, brilliantgreen, sabouraud's agar) of swabs and tracheal lavages following standardized sampling. respiratory symptoms were found in 24.3% of the snakes, 16.5% of the tortoises/turtles and 1.6% of the liz ... | 2007 | 17970338 |
| effect of eucalyptus essential oil on respiratory bacteria and viruses. | the activity of eucalyptus globulus essential oil was determined for 120 isolates of streptococcus pyogenes, 20 isolates of s. pneumoniae, 40 isolates of s. agalactiae, 20 isolates of staphylococcus aureus, 40 isolates of haemophilus influenzae, 30 isolates of h. parainfluenzae, 10 isolates of klebsiella pneumoniae, 10 isolates of stenotrophomonas maltophilia and two viruses, a strain of adenovirus and a strain of mumps virus, all obtained from clinical specimens of patients with respiratory tra ... | 2008 | 17972131 |
| cultivable bacteria associated with larval gut of prothiofos-resistant, prothiofos-susceptible and field-caught populations of diamondback moth, plutella xylostella and their potential for, antagonism towards entomopathogenic fungi and host insect nutrition. | to evaluate whether the gut bacteria of insecticide-resistant, insecticide-susceptible and field-caught populations of the lepidopteran insect pest diamondback moth (dbm)--plutella xylostella (l.)--are variable and their role in host protection and nutrition. | 2007 | 17973916 |
| [effect of the biofilm biopolymers on the microbial corrosion rate of the low-carbon steel]. | the relationship between exopolymer's specific production, relative carbohydrate and protein content in the biofilm exopolymers of the pure and mixed thiobacillus thioparus and stenotrophomonas maltophilia cultures and their corrosion activity was studied. change of growth model of investigated cultures from plankton to biofilm led to an increase of specific exopolymer's production. in the biofilm formed by t. thioparus and s. maltophilia biofilm on the low-carbon steel surface one could observe ... | 2007 | 17977451 |
| molecular typing of clinical isolates of stenotrophomonas maltophilia by pulsed-field gel electrophoresis and random primer pcr fingerprinting. | during a 1-year study period, 96 strains of stenotrophomonas maltophilia were isolated from different specimens of 59 patients admitted to a 1500 bed tertiary care university hospital. the strains were identified by commercially available api system and genotyped by pulsed-field gel electrophoresis (pfge) and random primer pcr fingerprinting. the wide variation of the molecular profiles of the investigated s. maltophilia isolates shown by pfge as well as pcr typing suggests that there was no out ... | 2008 | 17980662 |
| structural insights into the design of inhibitors for the l1 metallo-beta-lactamase from stenotrophomonas maltophilia. | one mechanism by which bacteria can escape the action of beta-lactam antibiotics is the production of metallo-beta-lactamases. inhibition of these enzymes should restore the action of these widely used antibiotics. the tetrameric enzyme l1 from stenotrophomonas maltophilia was used as a model system to determine a series of high-resolution crystal structures of apo, mono and bi-metal substituted proteins as well as protein-inhibitor complexes. unexpectedly, although the apo structure revealed on ... | 2008 | 17999929 |
| biodegradation of p-nitrophenol and 4-chlorophenol by stenotrophomonas sp. | a bacterium named lz-1 capable of utilizing high concentrations of p-nitrophenol (pnp) (up to 500 mg l(-1)) as the sole source of carbon, nitrogen and energy was isolated from an activated sludge. based on the results of phenotypic features and phylogenetic similarity of 16s rrna gene sequences, strain lz-1 was identified as a stenotrophomonas sp. other p-substituted phenols such as 4-chlorophenol (4-cp) were also degraded by strain lz-1, and both pnp and 4-cp were degraded via the hydroquinone ... | 2007 | 18031334 |
| in vitro activities of tigecycline combined with other antimicrobials against multiresistant gram-positive and gram-negative pathogens. | to test the activity of tigecycline combined with 16 antimicrobials in vitro against 22 gram-positive and 55 gram-negative clinical isolates. | 2008 | 18033780 |
| characterization of skin microbiota in patients with atopic dermatitis and in normal subjects using 16s rrna gene-based comprehensive analysis. | a previous study using bacterial 16s rrna gene-based clone libraries revealed that the microbiota in healthy human skin included uncultured micro-organisms, although the micro-organisms in skin exposed to disease conditions remain to be examined. to compare the profiles of skin microbiota in 13 patients with atopic dermatitis (ad) and 10 healthy controls, terminal rflp analysis of bacterial 16s rrna genes was applied to 23 swab-scrubbed samples from facial skin. this culture-independent analysis ... | 2007 | 18033838 |
| occurrence and antagonistic potential of stenotrophomonas strains isolated from deep-sea invertebrates. | stenotrophomonas maltophilia is known to be of significance as opportunistic pathogen as well as a source of biocontrol and bioremediation activities. s. maltophilia strains have been isolated from rhizospheres, soil, clinical material, aquatic habitats, but little is known about stenotrophomonas strains recovered from marine environments. during a survey of the biodiversity of pseudomonas-like bacteria associated with deep-sea invertebrates six stenotrophomonas strains were isolated from sponge ... | 2008 | 18034228 |
| diversity and antimicrobial susceptibility of oxytetracycline-resistant isolates of stenotrophomonas sp. and serratia sp. associated with costa rican crops. | to ameliorate the identification, evaluate the diversity, and determine the antimicrobial sensitivity of 19 oxytetracycline-resistant isolates of stenotrophomonas sp. and serratia sp. associated with costa rican crops. | 2007 | 18045439 |
| the role of horizontal transfer in the evolution of a highly variable lipopolysaccharide biosynthesis locus in xanthomonads that infect rice, citrus and crucifers. | lipopolysaccharide (lps) is a pathogen associated molecular pattern (pamp) of animal and plant pathogenic bacteria. variation at the interstrain level is common in lps biosynthetic gene clusters of animal pathogenic bacteria. this variation has been proposed to play a role in evading the host immune system. even though lps is a modulator of plant defense responses, reports of interstrain variation in lps gene clusters of plant pathogenic bacteria are rare. | 2007 | 18053269 |
| extracellular fatty acids facilitate flagella-independent translocation by stenotrophomonas maltophilia. | stenotrophomonas maltophilia is widespread in natural environments such as soil, sewage and plant rhizospheres. surfactants frequently function in modulating bacterial surface translocation. in this study, rpfb and rpff orthologues were identified from s. maltophilia strain wr-c, which was isolated from the clogged zone of a septic system. these genes play a role in the biosynthesis of eight extracellular compounds that facilitated flagella-independent translocation by the wild-type or a flagell ... | 2007 | 18054205 |
| substrate specificity of stenotrophomonas nitritireducens in the hydroxylation of unsaturated fatty acid. | an isolated bacterium that converted unsaturated fatty acids to hydroxy fatty acids was identified as stenotrophomonas nitritireducens by api analysis, cellular fatty acids compositions, sequencing the full 16s ribosomal ribonucleic acid, and evaluating its nitrite reduction ability. s. nitritireducens has unique regio-specificity for c16 and c18 cis-9 unsaturated fatty acids. these fatty acids are converted to their 10-hydroxy fatty acids without detectable byproducts. among the cis-9-unsaturat ... | 2008 | 18060400 |
| extremely late pacemaker-infective endocarditis due to stenotrophomonas maltophilia. | this case report describes a patient with an intravascular infection of a pacemaker system with stenotrophomonas maltophilia, which occurred 17 years after the implantation. the patient was treated with appropriate antibiotics and debridement of the infectious tissue in the pocket, and the entire pacemaker system was removed by open heart surgery. she was discharged from our center after a 6-week course of antibiotics and implantation of a new pacemaker. | 2008 | 18073476 |
| stenotrophomonas maltophilia and mycobacterium chelonae coinfection of the extraocular scleral buckle explant. | to report a very unusual form of coinfection of the extraocular scleral buckle explant by stenotrophomonas maltophilia and mycobacterium chelonae. | 2007 | 18085488 |
| induction of l1 and l2 beta-lactamases of stenotrophomonas maltophilia. | isogenic l1 and l2 gene knockout mutants of stenotrophomonas maltophilia kj (kjdeltal1 and kjdeltal2, respectively) were constructed by xyle gene replacement. induction kinetics of the l1 and l2 genes were evaluated by testing catechol 2,3-dioxygenase activity in the mutants. the results suggested that the induction of the l1 and l2 genes was differentially regulated. | 2008 | 18086856 |
| in vitro activity of isepamicin and other aminoglycosides against clinical isolates of gram-negative bacteria causing nosocomial bloodstream infections. | isepamicin is a newly introduced aminoglycoside in taiwan. since in vitro data for isepamicin against nosocomial gram-negative bloodstream infection from taiwan are limited, we compared the activity of isepamicin, amikacin, gentamicin and tobramycin against nosocomial gram-negative blood isolates. | 2007 | 18087627 |
| [pseudo-outbreak of stenotrophomonas maltophilia due to contamination of bronchoscope]. | we noticed an abrupt increase in the isolation of stenotrophomonas maltophilia from bronchoalveolar lavage (bal) specimens collected at chosun university hospital. we performed surveillance cultures in order to identify the source of what appeared to be a pseudo-outbreak. | 2007 | 18094577 |
| optimizing therapy for stenotrophomonas maltophilia. | stenotrophomonas (xanthomonas) maltophilia is a nonfermentative, gram-negative bacillus that is widely distributed in natural and human-made environments. in the nonhospital setting it is an uncommon pathogen, typically causing soft tissue infection of contaminated wounds. in the hospital setting, particularly among critical care and oncology patients, s. maltophilia may cause catheter-related bacteremia, pneumonia, soft tissue infection, meningitis, prosthetic valve endocarditis, and ocular inf ... | 2007 | 18095231 |
| n-demethylation of neonicotinoid insecticide acetamiprid by bacterium stenotrophomonas maltophilia cgmcc 1.1788. | our previous study found that stenotrophomonas maltophilia cgmcc 1.1788 could hydroxylate imidacloprid (imi) to 5-hydroxy imi. here we first report that s. maltophilia cgmcc 1.1788 can demethylate acetamiprid (aap) to form im 2-1 that was characterized by hplc-ms/ms and nmr. im 2-1 retained only 10.5% contact activity and 13.1% oral activity of aap against horsebean aphid. time course of biotransformation under existing of sucrose revealed that 58.9% of aap disappeared, but only 16.7% of reduced ... | 2008 | 18157735 |
| [invasive aspergillosis in an immunocompromised patient: clinical and therapeutic considerations]. | the paper describes a case report of a young female with invasive aspergillosis diagnosed after brief treatment with high-dose steroids for autoimmune thrombocytopenia. early diagnosis of invasive aspergillosis was made with cultures of tracheoaspirates and bronchoalveolar lavage and was confirmed with a transbronchial biopsy. after initial ineffective treatment with liposomal amphotericin b and dissemination from pulmonary to central nervous system involvement, treatment was switched to a combi ... | 2007 | 18162738 |
| appearance of multidrug-resistant opportunistic bacteria on the gingiva during leukemia treatment. | dentists generally recognize the importance of periodontal treatment in patients with leukemia, with the most attention paid to preventing the development of odontogenic infection. for physicians, the worst type of infection is one caused by multidrug-resistant bacteria. here, we report a patient with an abnormal increase in multidrug-resistant opportunistic bacteria in the gingiva during hematopoietic cell transplantation (hct). | 2008 | 18166109 |
| [surveillance of antimicrobial resistance among nosocomial gram-negative pathogens from 15 teaching hospitals in china in 2005]. | to investigate the antimicrobial resistance among the nosocomial gram-negative pathogens from 15 teaching hospitals located in different areas in china in 2005. | 2007 | 18167265 |
| stenotrophomonas maltophilia responsible for respiratory infections in neonatal intensive care unit: antibiotic susceptibility and molecular typing. | the aim of this study was to investigate the molecular epidemiology of stenotrophomonas maltophilia strains responsible for respiratory infection in a neonatal intensive care unit (nicu) in tunis city, isolated during 22 months (december 2003-september 2005). | 2009 | 18178029 |
| salvage of long-term central venous catheters during an outbreak of pseudomonas putida and stenotrophomonas maltophilia infections associated with contaminated heparin catheter-lock solution. | to describe the management of patients with long-term central venous catheters (cvcs) during an outbreak of infection due to pseudomonas putida and stenotrophomonas maltophilia associated with contaminated heparin catheter-lock solution. | 2008 | 18179367 |
| microbiologic study of soft contact lenses after laser subepithelial keratectomy for myopia. | to evaluate the extent and agents of bacterial contamination of bandage disposable soft contact lenses after laser subepithelial keratectomy (lasek) and to correlate the findings with clinical data. | 2008 | 18180679 |
| resistance in gram-negative bacilli in a cardiac intensive care unit in india: risk factors and outcome. | the objective of this study was to compare the risk factors and outcome of patients with preexisting resistant gram-negative bacilli (gnb) with those who develop sensitive gnb in the cardiac intensive care unit (icu). of the 3161 patients ( n=3,161) admitted to the icu during the study period, 130 (4.11%) developed health care-associated infections (hais) with gnb and were included in the cohort study. pseudomonas aeruginosa (37.8%) was the most common organism isolated followed by klebsiella sp ... | 2008 | 18182755 |
| stenotrophomonas maltophilia: changing spectrum of a serious bacterial pathogen in patients with cancer. | stenotrophomonas maltophilia colonization/infection in patients with cancer has significantly increased over the past 2 decades. patients with prolonged neutropenia, exposure to broad-spectrum antibiotics, and those requiring mechanical ventilation have higher risk of infection. these micro-organisms are intrinsically resistant to carbapenems, and exposure to these agents has been linked to selection of s. maltophilia. recently, these infections are being documented in patients without tradition ... | 2007 | 18190323 |
| conversion of linoleic acid into 10-hydroxy-12(z)-octadecenoic acid by whole cells of stenotrophomonas nitritireducens. | the conversion of linoleic acid into 10-hydroxy-12(z)-octadecenoic acid by whole cells of stenotrophomonas nitritireducens as an isolated bacterium was optimized, and the optimal temperature, ph, and cell and substrate concentrations were 30 degrees c, 7.5, and 20 and 20 g/l, respectively. under these conditions, whole cells in a bioreactor produced 15 g/l 10-hydroxy-12(z)-octadecenoic acid in 2 h of reaction time without detectable byproducts. using 2 g/l linoleic acid, the cells produced 1.92 ... | 2008 | 18197675 |
| induction of l1 and l2 beta-lactamase production in stenotrophomonas maltophilia is dependent on an ampr-type regulator. | a divergently oriented ampr has been located upstream of blal2 in stenotrophomonas maltophilia. ampr is necessary for l1 and l2 beta-lactamase induction in response to beta-lactam challenge, and activation of ampr is sufficient to induce l1 and l2 production. l1 induction requires more activation of ampr than does l2 induction. | 2008 | 18212097 |
| posttraumatic stenotrophomonas maltophilia infectious scleritis. | to describe the history, clinical presentation, and successful surgical and antibiotic management of a case of posttraumatic infectious scleritis secondary to stenotrophomonas maltophilia. | 2008 | 18216585 |
| [the vacuum-assisted closure (v.a.c.) and instillation dressing: limb salvage after 3 degrees open fracture with massive bone and soft tissue defect and superinfection]. | we report the case of a 17-year-old boy who was hit by a high velocity train. the polytraumatized patient suffered a 3 degrees open femur defect fracture with a substantial loss of the lateral femoral muscles and significant disruption of the soft tissue of the lower leg. the enormous wound areas on the thigh and the lower leg were infected by pseudomonas aeruginosa, enterobacter cloacae, and stenotrophomonas maltophilia. the enormous tissue defects and the superinfection did not leave any hope ... | 2008 | 18219474 |
| decontamination effects of low-temperature plasma generated by corona discharge. part ii: new insights. | the second part of our paper presents the results of experiments with the decontamination of surfaces by low-temperature plasma generated by corona discharge in air at atmospheric pressure. a simple device is described and the effects of the corona discharge on model microorganisms, viz. the yeast candida albicans, gram-negative bacteria escherichia coli, enterobacter aerogenes, neisseria sicca, stenotrophomonas maltophilia, gram-positive bacteria deinococcus radiodurans, enterococcus faecium, s ... | 2007 | 18225640 |
| clinical aspects and risk factors of nosocomial stenotrophomonas maltophilia bacteremia episodes in a turkish intensive care unit. | stenotrophomonas maltophilia is an important nosocomial pathogen with increasing frequency in recent years, especially in immunocompromised and clinically debilitated patients. the aim of this study is to describe the characteristics of 35 episodes of s. maltophilia bacteremia at celal bayar university hospital in turkey over a 3-year period from january 2003 to december 2005. cases were identified with microbiology laboratory records and clinical data were collected from the medical record of e ... | 2007 | 18230546 |
| pseudooutbreak of stenotrophomonas maltophilia bacteremia in a general ward. | stenotrophomonas maltophilia is a significant nosocomial pathogen and is commonly isolated in the hospital environment. we investigated the risk factors for a bloodstream pseudooutbreak of s maltophilia in a general ward. | 2008 | 18241733 |
| fluorescence correlation spectroscopy to study diffusion and reaction of bacteriophages inside biofilms. | in the natural environment, most of the phages that target bacteria are thought to exist in biofilm ecosystems. the purpose of this study was to gain a clearer understanding of the reactivity of these viral particles when they come into contact with bacteria embedded in biofilms. experimentally, we quantified lactococcal c2 phage diffusion and reaction through model biofilms using in situ fluorescence correlation spectroscopy with two-photon excitation. correlation curves for fluorescently label ... | 2008 | 18245240 |
| heavy metal tolerance in stenotrophomonas maltophilia. | stenotrophomonas maltophilia is an aerobic, non-fermentative gram-negative bacterium widespread in the environment. s. maltophilia sm777 exhibits innate resistance to multiple antimicrobial agents. furthermore, this bacterium tolerates high levels (0.1 to 50 mm) of various toxic metals, such as cd, pb, co, zn, hg, ag, selenite, tellurite and uranyl. s. maltophilia sm777 was able to grow in the presence of 50 mm selenite and 25 mm tellurite and to reduce them to elemental selenium (se(0)) and tel ... | 2008 | 18253487 |
| stenotrophomonas maltophilia endocarditis treated with moxifloxacin-ceftazidime combination and annular wrapping technique. | | 2008 | 18258545 |
| assessment of microbial diversity in effluent treatment plants by culture dependent and culture independent approaches. | microbial community structure of two distinct effluent treatment plants (etps) of pesticide and pharmaceutical industries was assessed and defined by (i) culture dependent and culture independent approaches on the basis of 16s rrna gene sequencing, (ii) diversity index analysis - operational taxonomic units (otus). a total of 38 and 44 bacterial otus having 85-99% similarity with the closest match in the database were detected among pharmaceutical and pesticide sludge samples, respectively. fift ... | 2008 | 18280146 |
| catalase and superoxide dismutase activities in a stenotrophomonas maltophilia wz2 resistant to herbicide pollution. | quinclorac bensulfuron-methyl is a mixed herbicide widely used on paddy rice field to effectively control barnyard grass and most broad-leaved grasses and sedges. we analyzed superoxide dismutase (sod) and catalase activities in the quinclorac-highly degrading strain stenotrophomonas maltophilia wz2 and gram-negative standard strain escherichia coli k12 in an attempt to understand antioxidant enzymes in bacteria are produced in response to quinclorac or bensulfuron-methyl, which increases the vi ... | 2009 | 18304632 |
| interspecies signalling via the stenotrophomonas maltophilia diffusible signal factor influences biofilm formation and polymyxin tolerance in pseudomonas aeruginosa. | interspecies signalling through the action of diffusible signal molecules can influence the behaviour of organisms growing in polymicrobial communities. stenotrophomonas maltophilia and pseudomonas aeruginosa occur ubiquitously in the environment and can be found together in diverse niches including the rhizosphere of plants and the cystic fibrosis lung. in mixed species biofilms, s. maltophilia substantially influenced the architecture of p. aeruginosa structures, which developed as extended fi ... | 2008 | 18312265 |
| survey of bacterial diversity in chronic wounds using pyrosequencing, dgge, and full ribosome shotgun sequencing. | chronic wound pathogenic biofilms are host-pathogen environments that colonize and exist as a cohabitation of many bacterial species. these bacterial populations cooperate to promote their own survival and the chronic nature of the infection. few studies have performed extensive surveys of the bacterial populations that occur within different types of chronic wound biofilms. the use of 3 separate16s-based molecular amplifications followed by pyrosequencing, shotgun sanger sequencing, and denatur ... | 2008 | 18325110 |
| colistin susceptibility testing by etest and disk diffusion methods. | the accuracy of disk susceptibility methods for colistin against 778 bacterial pathogens was evaluated in comparison with etest using interpretive criteria available from the clinical and laboratory standards institute (clsi). colistin exhibited excellent activity against acinetobacter baumannii and escherichia coli isolates (minimum inhibitory concentration for 90% of the organisms (mic(90))=0.5 mg/l), whilst it was less active both against enterobacter spp. and klebsiella pneumoniae (mic for 5 ... | 2008 | 18328674 |
| antimicrobial susceptibility profile of contemporary clinical strains of stenotrophomonas maltophilia isolates: can moxifloxacin activity be predicted by levofloxacin mic results? | the susceptibility profile of 763 stenotrophomonas maltophilia isolates was evaluated against 16 antimicrobials by the clsi reference broth microdilution method. minocycline (mic(90), 1 microg/ml; 100.0% susceptible) was the most active compound, followed by doxycycline (mic(90), 4 microg/ml; 99.6% susceptible), trimethoprim/sulfamethoxazole (mic(90), 1 microg/ml; 97.8% susceptible), and tigecycline (mic(90), 2 microg/ml). an excellent correlation between levofloxacin (mic(90), 4 microg/ml; 86.5 ... | 2008 | 18343741 |
| water filters can prevent stenotrophomonas maltophilia contamination of nebuliser equipment used by people with cystic fibrosis. | | 2008 | 18353498 |
| [monosaccharide composition of exopolymer complex in thiobacillus thioparus and stenotrophomonas maltophilia]. | the bacteria of thiobacillus thioparus and stenotrophomonas maltophilia are capable to form a thick biofilm complicated as to its structure on the surface of low-carbon steel. this biofilm formation on any surface occurs under the adhesion of the cells of the plankton growth model with the help of synthesis of muciferous exopolymers with adhesive properties. hence the monosaccharide composition of the exopolymer complex in the form of polyol acetates was studied by chromate-mass-spectrum method. ... | 2007 | 18357787 |
| community-acquired infection due to stenotrophomonas maltophilia: a rare cause of septic arthritis. | stenotrophomonas maltophilia is an important nosocomial pathogen in hospitalized patients, particularly those with prior broad-spectrum antibacterial therapy. the microorganism mainly infects severely ill, debilitated patients and is most frequent in immunocompromised hosts. a prominent feature of this organism is its resistance to multiple antibiotics including beta-lactam agents, carbapenems and aminoglycosides. community-acquired infection with stenotrophomonas maltophilia is reported rarely. ... | 2008 | 18365601 |
| transcriptional analysis and functional characterization of a gene pair encoding iron-regulated xenocin and immunity proteins of xenorhabdus nematophila. | we describe a two-gene cluster encoding a bacteriocin, xenocin, and the cognate immunity protein in the insect-pathogenic bacterium xenorhabdus nematophila, which infects and kills larval stages of the common crop pest helicoverpa armigera. the two genes, xcina and ximb, are present in the genome as a single transcriptional unit, which is regulated under sos conditions. the stress-inducible promoter was activated by mitomycin c, glucose, and fe(3+) depletion and at an elevated temperature when i ... | 2008 | 18375563 |
| new insights into a bacterial metabolic and detoxifying association responsible for the mineralization of the thiocarbamate herbicide molinate. | a novel pathway of molinate mineralization promoted by a defined mixed culture composed of five bacteria (named on1 to on5) was proposed previously. evidence was obtained of a metabolic association between gulosibacter molinativorax on4(t), capable of molinate breakdown, and the remaining bacteria. in the present study, the role of each isolate in that metabolic association was further explored and the possible synergistic effect of all the bacterial isolates for the stability of the mixed cultu ... | 2008 | 18375797 |
| [new options for treatment of intraabdominal infections: tigecycline]. | tigecycline is a new antimicrobial agent; it is the first in a new class of antibiotics, the glycylcyclines, with properties conferring the ability to overcome many common resistance mechanisms, thus allowing its use for many serious and life-threatening infections for which the use of other antibiotics is no longer appropriate. it has a wide antibacterial spectrum including most methicillin-resistant staphylococci, vancomycin-resistant enterococci, esbl-producing gram negative bacteria, and oth ... | 2008 | 18382151 |
| biofilm formation by stenotrophomonas maltophilia isolates from device-associated nosocomial infections. | medical devices are often colonized by bacteria which may cause severe infections. the aim of this work was to evaluate biofilm formation by s. maltophilia isolates from device-associated nosocomial infections. the 13 local isolates exhibited different capacities of biofilm formation on hydrophilic and hydrophobic surfaces. all isolates formed strong biofilms in polystyrene microplates, while strong, moderate or weak biofilms were detected in borosilicate (bs) or polypropylene (pp) tubes. the pr ... | 2007 | 18390153 |
| bacterial ectosymbionts and virulence silencing in a fusarium oxysporum strain. | in the present article we have ascertained the presence of a consortium of ectosymbiotic bacteria belonging to serratia, achromobacter, bacillus and stenotrophomonas genera associated to the mycelium of the antagonistic fusarium oxysporum msa 35 [wild-type (wt) strain]. morphological characterization carried out on the wt strain, on the f. oxysporum msa 35 without ectosymbionts [cured (cu) strain] and on the pathogenic f. oxysporum f.sp. lactucae (fuslat 10) showed that the ectosymbionts, presen ... | 2008 | 18397306 |
| hospital tap water as a source of stenotrophomonas maltophilia infection. | | 2008 | 18419467 |
| the complete genome, comparative and functional analysis of stenotrophomonas maltophilia reveals an organism heavily shielded by drug resistance determinants. | stenotrophomonas maltophilia is a nosocomial opportunistic pathogen of the xanthomonadaceae. the organism has been isolated from both clinical and soil environments in addition to the sputum of cystic fibrosis patients and the immunocompromised. whilst relatively distant phylogenetically, the closest sequenced relatives of s. maltophilia are the plant pathogenic xanthomonads. | 2008 | 18419807 |
| adaptive plasmid evolution results in host-range expansion of a broad-host-range plasmid. | little is known about the range of hosts in which broad-host-range (bhr) plasmids can persist in the absence of selection for plasmid-encoded traits, and whether this "long-term host range" can evolve over time. previously, the bhr multidrug resistance plasmid pb10 was shown to be highly unstable in stenotrophomonas maltophilia p21 and pseudomonas putida h2. to investigate whether this plasmid can adapt to such unfavorable hosts, we performed evolution experiments wherein pb10 was maintained in ... | 2008 | 18430943 |
| application of a magnetotactic bacterium, stenotrophomonas sp. to the removal of au(iii) from contaminated wastewater with a magnetic separator. | in this study, the feasibility of applying a magnetotactic bacterial isolate (mtb), stenotrophomonas sp. to the removal of au(iii) was investigated. biosorption experiments showed that au(iii) biosorption capacity exhibited no significant difference in the initial ph range of 1.0-5.5, while decreased more significantly in the initial ph range of 5.5-13.0. langmuir isotherm indicated that the maximum au(iii) biosorption capacity of stenotrophomonas sp. were 506, 369 and 308 mg g(-1) dry weight bi ... | 2008 | 18439649 |
| a convenient microbiological assay employing cell-free extracts for the rapid characterization of gram-negative carbapenemase producers. | the dissemination of metallo and serine carbapenem-hydrolysing beta-lactamases among gram-negative nosocomial bacteria represents an acute problem worldwide. here, we present a rapid and sensitive assay for the characterization of carbapenemase producers to aid in infection control and prevention. | 2008 | 18456651 |
| effects of fluoroquinolones on bacterial adhesion and on preformed biofilm of strains isolated from urinary double j stents. | the activity of levofloxacin and ulifloxacin on biofilm formation and persistence was evaluated on microorganisms isolated from urinary double-j-stents. we analyzed 51 bacterial strains and their susceptibility to different antimicrobial classes was determined. we evaluated the bacterial ability to form biofilm and the effects of different concentrations of levofloxacin and ulifloxacin on bacterial adhesion and biofilm persistence. most of the strains were biofilm producers with no relevant diff ... | 2008 | 18467245 |
| bsac standardized disc susceptibility testing method (version 7). | the changes that have been made to the previous version of the recommendations (version 6) are as follows: medium and incubation condition for testing acinetobacter spp. (tables 1 and 6); use of cefoxitin as an indicator antibiotic for detecting methicillin/oxacillin/cefoxitin resistance in coagulase-negative staphylococci (tables 1, 6 and 11); mic breakpoint for co-trimoxazole based on the trimethoprim concentration in a 1:19 combination with sulfamethoxazole (tables 7, 10, 11, 12, 15, 16 and 1 ... | 2008 | 18474513 |