| [high-performance exclusion liquid chromatography of protected peptides on soft and semi-solid gels]. | fractionated soft gels sephadex lh-20, sephadex lh-60, enzakryl k-2 and semirigid gels - spheron p-40, spheron p-300 and spheron p-1000 were tested for separation of protected synthetic fragments of neurotoxin ii from the central asian cobra (naja naja oxiana) venom. applicability of the above gels for size exclusion hplc of protected peptides in organic media is discussed. | 1983 | 6679779 |
| [preparation and esr study of spin-labeled derivatives of naja naja oxiana neurotoxin ii]. | after neurotoxin ii naja naja oxiana reaction with n-hydroxysuccinimidyl 2,2,6,6-tetramethyl-4-carboxymethylpiperidine-1-oxyl, six derivatives were isolated, each containing one spin label. their analysis (reduction, carboxymethylation, tryptic hydrolysis, isolation and identification of the spin labeled peptide) allowed to localize the label position: the epsilon-amino groups of lys15, lys25, lys26, lys44, lys48, and alpha-amino group of leu1. the neurotoxin ii reaction with n-hydroxysuccinimid ... | 1983 | 6091692 |
| ultracytochemical observations on intracytoplasmic lipids by enzymic digestive methods: histochemical trials1. | the penetration of rbizopus arrhizus lipase into ethioine fatty livers was examined histochemically. a free-floating, 30 μm thick vibratome section of the livers perfused with aldehydes was shown to be suitable for ultracytochemical analysis by the lipase digestive method. accessibility of phospholipids in the outer membrane of erythrocytes to naja naja phospholipase a2was observed under a light microscope, and intact red cells were used for the ultracytochemical analysis by the phospholipase di ... | 1983 | 28054839 |
| an attempt to identify amino groups of naja naja siamensis neurotoxin that interact with acetylcholine receptor by a comparison of their reactivities in free and receptor-bound neurotoxin. | an attempt to identify amino groups of naja naja siamensis neurotoxin that interact with acetylcholine receptor by a comparison of their reactivities in free and receptor bound neurotoxin. toxicon 21, 219-229, 1983--free naja naja siamensis neurotoxin was acetylated with non-radioactive and acetylcholine receptor-bound neurotoxin with radioactive acetic anhydride. the toxins from the two experiments were combined and the monoacetyl derivatives isolated by chromatography on bio-rex 70. the yields ... | 1983 | 6857707 |
| fractionation of protein components of plasma membranes from the electric organ of torpedo marmorata. | a procedure has been developed for the separation of intrinsic proteins of plasma membranes from the electric organ of torpedo marmorata. (na+ + k+)-atpase, nicotinic acetylcholine receptor and acetylcholinesterase remained active after solubilization with the nonionic detergent dodecyl octaethylene glycol monoether (c12e8). these components could be separated by ion exchange chromatography on deae-sephadex a-25. fractions enriched in ouabain-sensitive k+-phosphatase or (na+ + k+)-atpase activit ... | 1982 | 6297554 |
| hemolytic properties of membrane-active polypeptides from the venom of central asian cobra (naja naja oxiana eichwald). | | 1982 | 7184846 |
| cobra venom acetylcholinesterase: nature of charge isoforms. | charge isoforms of cobra (naja naja oxiana) venom acetylcholinesterase, separated by isoelectric focusing, differ only by the number of free carboxyl groups of glutamic and/or aspartic acid side-chains in the enzyme molecule. the isoforms appear to be produced by a post-translational deamidation of accessible glutamin and/or asparagine residues. the isoforms have identical catalytic specificities towards characteristic acetylcholinesterase substrates. | 1982 | 7173192 |
| site-specific fluorescein-labeled cobra alpha-toxin. biochemical and spectroscopic characterization. | cobra alpha-toxin purified from naja naja siamensis venom was labeled with near stoichiometric quantities of fluorescein isothiocyanate. a monofluorescein alpha-toxin was separated in 50-60% yield from unconjugated alpha-toxin and other reaction products by ion exchange chromatography. the isolated mono-conjugated alpha-toxin electrofocuses largely as a single entity with 92% appearing with a pi of 9.6. the unmodified toxin has a pi of 10.7. thermolysin digestion and subsequent high pressure liq ... | 1982 | 6802819 |
| role of monomeric activators in cobra venom phospholipase a2 action. | phospholipase a2 from cobra venom (naja naja naja), which acts poorly on phosphatidylethanolamine (pe) in mixed micelles, is activated toward pe by the monomeric phospholipid dibutyrylphosphatidylcholine (dibutyryl-pc) which is an, even poorer substrate. phosphorus-31 nuclear magnetic resonance spectroscopy was employed to show that only pe is hydrolyzed in mixtures of pe and dibutyryl-pc of various concentrations. the activation shows saturation behavior, and the fully activated enzyme hydrolyz ... | 1982 | 7082644 |
| studies on snake venom enzymes: part i. purification of atpase, a toxic component of naja naja venom & its inhibition by potassium gymnemate. | | 1982 | 6215333 |
| specific binding to isolated acetylcholine receptor of a synthetic peptide duplicating the sequence of the presumed active center of a lethal toxin from snake venom. | to verify the existence of a lethal "active center" in snake venom neurotoxins and to assess its delineation within their polypeptide sequences, a tritriacontapeptide matching residues 16-48 of the natural "major" toxin of naja naja philippinensis (hauert, j., maire, m., sussmann, a., and bargetzi, j. p. (1974) int. j. pept. protein research 6, 201-222) has been synthesized by solid-phase technology (juillerat, m. a., and bargetzi, j. p. (1980), results presented at the 16th european peptide sym ... | 1982 | 7061455 |
| local conformational transition of toxin b from naja naja as studied by nuclear magnetic resonance and circular dichroism. | the 270-mhz proton nmr spectra of toxin b, a long neurotoxin from naja naja, in 2h2o solution were analyzed. from the analysis of the discontinuous ph dependence of his-21 c-2 proton chemical shift a 23 degrees c, toxin b is found to exhibit a slow-exchange conformational transition between the states a and b with the transition midpoint at ph 5.75. separate peaks due to the states a and b were also observed for his-21 c-4 proton, tyr-25 ring protons, phenylalanine ring protons and two c-alpha p ... | 1982 | 7060589 |
| interaction surfaces of neurotoxins and acetylcholine receptor. | binding of neurotoxin ii naja naja oxiana derivatives containing one spin label at various positions (leu 1, glu 2, lys 15, lys 25, lys 26, his 31, lys 44 and lys 46) to purified solubilized acetylcholine receptor protein (achr) from torpedo marmorata was studied by epr techniques. achr interaction with several dansylated neurotoxin ii derivatives was followed by difference fluorescence spectroscopy. a series of neurotoxin ii p-azidobenzoyl derivatives were prepared and in three of them modified ... | 1982 | 7080049 |
| electron microscopy of complexes of isolated acetylcholine receptor, biotinyl-toxin, and avidin. | the principal curarimimetic toxin of naja naja siamensis derivatized with biotinyl groups binds specifically both to acetylcholine receptor, isolated from torpedo californica electric tissue, and to avidin. isolated complexes of receptor monomer or dimer, biotinyl-toxin, and avidin were negatively stained and examined in the scanning transmission electron microscope. we measured the angle made by the radius of each avidin bound at the periphery of a monomeric unit in dimer to the axis connecting ... | 1982 | 6952187 |
| the amino acid sequence of toxin d isolated from the venom of indian cobra (naja naja). | a neurotoxin, designated toxin d, was isolated from the venom of naja naja by gel-filtration on sephadex g-50 followed by cm-cellulose chromatography. the amino acid sequence of toxin d was determined by analyzing tryptic and chymotryptic peptides of the reduced and s-carboxymethyl derivative. the venom contains at least four long (toxins a, b, c and d) and two short neurotoxins (toxins i and ii) that account for about 16% of the lyophilized crude venom by weight. toxin d consists of 71 amino ac ... | 1981 | 7326259 |
| 19f nmr determination of intramolecular distances in spin- and fluorine-labelled proteins: neurotoxin ii naja naja oxiana. | | 1981 | 7327263 |
| six polymorphic crystal forms of alpha-cobratoxin. | six different crystal forms of the long neurotoxin alpha-cobratoxin isolated from the venom of naja naja siamensis have been obtained and the conditions for their crystallisation are described. heavy atom derivatives for two of these forms have been prepared and have proven suitable for high-resolution x-ray studies and, with one of them, a 0.28-nm resolution structure has been determined. all but one of the crystal forms were grown between ph 2 and ph 3. two novel suggestions are made which may ... | 1981 | 7308208 |
| cobra venom phospholipase a2: a review of its action toward lipid/water interfaces. | this review focuses on the mechanism of action of phospholipase a2 from cobra venom (naja naja naja) toward the lipid/water interface. particular points of interest include dramatic changes in the enzyme activity if the physical state of its substrate is altered and the activation of the enzyme by phosphorylcholine containing lipids. the experimental findings include the following: micellar substrates are hydrolyzed faster by the enzyme than various bilayer forms of substrate aggregation. the ac ... | 1981 | 7242529 |
| first aid for cobra (naja naja) bites. | | 1981 | 7239610 |
| molecular characterization of the complement activating protein in the venom of the indian cobra (naja n. siamensis). | | 1981 | 6790937 |
| phospholipase a2 from cobra venom (naja naja naja). | | 1981 | 7278679 |
| source of carbohydrate in a toxic protein from indian cobra (naja naja naja) venom. | | 1981 | 7245223 |
| cytoarchitectonic pattern of the hypothalamus in the cobra, naja naja. | the distribution and cytoarchitectonic pattern of the magno- and parvocellular hypothalamic nuclei of the cobra. naja naja, are described at the light-microscopic level. with respect to their tinctorial affinity to paraldehyde fuchsin (af) as a representative of the gomori-type of stains, the magnocellular neurons belong to the "af-positive" and the parvocellular neurons to the "af-negative" elements. in addition to the supraoptic and paraventricular nuclei proper, two accessory aggregations of ... | 1981 | 6166381 |
| [observations on cytolysis by cobra venom. effect of sucrose loading]. | ls cells cultivated for 4 days in sucrose-containing medium (su) are more fragile than cells cultivated in medium devoid of this sugar (tn) when they are incubated with naja-naja venom; in fact the 51cr radioactivity released into the medium by previously labelled cells is increased for the sucrose ones. phospholipase does not seem to contribute to the lytic activity of the venom, which is dependent on the direct lytic factor: neither varying the temperature of incubation nor adding edta to the ... | 1980 | 7459127 |
| transverse distribution of phospholipids in organelle membranes from ricinus communis l. var. hale endosperm: mitochondria and glyoxysomes. | phospholipase a(2) (naja naja), the nonpenetrating dye trinitrobenzene sulfonate, and the penetrating dye dinitrofluorobenzene, were used to determine the transmembrane distributions of phospholipids of mitochondria and glyoxysomes isolated from endosperm tissue of castor bean (ricinus communis l. var. hale). these studies indicated that the phospholipid distributions were distinctly asymmetric in the accessible (reacted with the probes without total membrane disruption by detergents) pools of t ... | 1980 | 16661334 |
| a single-step separation procedure for several protein constituents of venom of the indian cobra (naja naja). | | 1980 | 7450809 |
| [phospholipase spin-labeled phospholipids interactions]. | enzyme-substrate interaction of phospholipase a2 (naja naja oxiana) with phospholipids has been studied. spin-labeled palmitic and stearic acids, 1-o-spin-labeled acyl-, 2-o-spin-labeled acylphosphatidyl choline and spin-labeled phosphatidyl-myo-inositol were used for this purpose. this method did not reveal hydrofobic fat-protein interaction. phospholipase interacts only with the near lipid monolayer of liposomes and vesicles. being formed lysophosphatidyl choline and fat acids lead to destruct ... | 2017 | 7402203 |
| three-dimensional structure of the "long" neurotoxin from cobra venom. | the three-dimensional structure of alpha-cobra-toxin, the "long" neurotoxin from the venom of naja naja siamensis, has been determined at 2.8-a resolution. crystals grown as hexagonal needles have space group p6522 with unit cell parameters a = b = 74.59 a, c = 42.89 a; one molecule per asymmetric unit. phases were determined with a single isomorphous derivative with hgi2 by using the anomalous scattering of the single-site hgi2 molecule to resolve the phase ambiguity. the polypeptide chain fold ... | 1980 | 6930640 |
| interaction of spin-labeled naja naja siamensis alpha-neurotoxin with acetylcholine receptor from torpedo californica. | | 1980 | 7371834 |
| purification of two phospholipase a isoenzymes with anticoagulant activity from the venom of the cobra naja naja siamensis. | | 1980 | 7210026 |
| epr and fluorescence study of interaction of naja naja oxiana neurotoxin ii and its derivatives with acetylcholine receptor protein from torpedo marmorata. | | 1979 | 227728 |
| photoaffinity derivatives of alpha-bungarotoxin and alpha-naja naja siamensis toxin. | | 1979 | 467650 |
| cobra venom acetylcholinesterase. purification and molecular properties. | acetylcholinesterase from cobra (naja naja oxiana) venom has been purified by affinity chromatography to an homogeneous state, as ascertained by sodium dodecylsulfate/polyacrylamide gel electrophoresis and sedimentation analysis. the specific activity of the preparation was 5000 iu/mg with acetylcholine as substrate. unlike acetylcholinesterases from insoluble cell structures, the native molecule of the cobra venom enzyme consists of a single polypeptide chain of molecular weight 67,000 +/- 2000 ... | 1979 | 456362 |
| conformational studies of neurotoxin ii from naja naja oxiana. selective n-acylation, circular dichroism and nuclear-magnetic-resonance study of acylation products. | after treatment of neurotoxin ii, a component part of the venom of the middle asian cobra naja naja oxiana, with acetoxysuccinimide all five possible epsilon-acetylated-lysyl derivatives were obtained and the position of the label was established. trifluoroacetylation of both the derivatives and the parent toxin yielded, respectively, the five acetyl-penta(trifluoroacetyl)-neurotoxins ii and the hexa(trifluoroacetyl)-neurotoxin ii, which were studied by circular dichroism (cd), 1h and 19f nuclea ... | 1979 | 34515 |
| an improved method for the isolation from naja naja venom of cobra factor (cof) free of phospholipase a. | an improved method is reported for the isolation from cobra (naja naja) venom of cobra factor (cof), the anticomplementary protein which is derived from cobra c3. sequential chromatography on deae-sepharose, sephacryl-s200, and finally hydroxylapatite yielded 6.25 mg cof per gram of crude venom. the purified cof had 1 unit of functional anticomplementary activity per 1--2 micrograms of protein, and was homogeneous on gradient and non-reduced sodium dodecyl sulphate (sds) polyacrylamide gel elect ... | 1979 | 574149 |
| [interaction of cardiotoxin from the venom of the cobra naja naja oxiana with phospholipid membrane model systems]. | surface activity of cardiotoxin from the venom of cobra naia naja oxiana and an acetylated analog of the cardiotoxin was investigated on the water-air interface. the interaction of these substances with lipid monolayers and bimolecular membrane was also studied. it was shown that on the water-air interface cardiotoxin can form two different types of monolayers with different conformations of molecules. cardiotoxin is found to form complexes with phospholipids due to interaction of positive charg ... | 2017 | 739993 |
| binding of thiamine to nicotinic acetylcholine receptor in torpedo marmorata and the frog end plate. | thiamine was found to bind to the isolated nicotinic receptor (nachr) from torpedo marmorata (kd = 3--5 x 10(-5) m). the binding was reversible and inhibited by alpha-neurotoxin from naja naja siamensis. acetylcholine binding was not inhibited by thiamine. when thiamine (5 x 10(-4) m) was applied to frog sartorius muscles a decrease of miniature end plate potential amplitudes was seen. this effect was readily reversible after the perfusion fluid of the muscle was changed to normal ringer solutio ... | 1978 | 307899 |
| an electron microscopic study of the adductor externus superficialis muscle associated with the venom gland of the cobra (naja naja). | the adductor externus superficialis muscle closely envelops the venom gland in the cobra (naja naja). only twitch fibres are present in the muscle, supporting the view that the muscle is concerned with the rapid evacuation of venom. 'satellite' cells are readily observed in relation to the muscle fibres. | 1978 | 649503 |
| membranes rich in acetylcholine receptor: characterization and reconstitution to excitable membranes from exogenous lipids. | characterization of acetylcholine-receptor-enriched membranes from torpedo californica electric tissue by negative-staining electron-microscopy and by lipid analysis is described. the protein/lipid ratio is 70%/30%. the lipids consist of 70% phospholipids (46% phosphatidylcholine, 31% phosphatidylethanolamine, 14% phosphatidylserine, 7% sphingomyelin, 2% phosphatidylinositol of the phospholipids determined) and 20% cholesterol. the acetylcholinesterase-enriched membranes show a similar compositi ... | 1978 | 639824 |
| clinical observations on glucocorticoids in cobra envenomation. | a clinical trial of corticosteroid treatment in five cases of cobra, naja naja, bite with systemic poisoning is reported. the results reveal that corticosteroid had no demonstrable beneficial effect in the neurotoxic poisoning of the cobra. specific antivenom is the most important therapeutic agent for systemic poisoning. the combination of antivenom and corticosteroid had no effect on the development of local necrosis. | 1978 | 705419 |
| a simple method for purification of cardiotoxin from indian cobra (naja naja naja) venom. | | 1978 | 684770 |
| mechanism of phospholipase a2 action toward mixed micelles of detergent and phospholipids. | phospholipase a2 from cobra venom (naja naja naja) is a homogeneous, heat-stable enzyme that has a monomer molecular weight of only 11,000 and contains one histidine and one tryptophan residue. this enzyme acts optimally on phospholipids contained in mixed micelles with the nonionic detergent triton x-100; the interactions of this detergent with phospholipid in the mixed micelles have been elucidated with 1h- and 13c-nmr. in reacting, the enzyme first associates with the mixed micelle and then e ... | 1978 | 665362 |
| reversible neuromuscular blockade by modified neurotoxin from naja naja siamensis (39875). | | 1977 | 143665 |
| studies on the reproductive cycle on the female cobra, naja naja. ii. in vitro ovarian steroid synthesis. | | 1977 | 142685 |
| chemical modification of the histidine residue in phospholipase a2 (naja naja naja). a case of half-site reactivity. | reaction of phospholipase a2 (naja naja naja) with p-bromophenacyl bromidine leads to almost complete loss of enzymatic activity. the rate of inactivation is ph-dependent with pka equals 6.9 for the ionizing residue. p-bromophenacyl bromide modifies 0.5 mol of histidine/mol of enzyme as judged by amino acid analysis and incorporation studies with 14c-labeled reagent. the rate of inactivation is affected by various cations; a saturating concentration of ca2+ decreases the rate 5-fold, while mn2+ ... | 1977 | 14964 |
| [effect of diacid analogs of phosphatidylcholines on phospholipase a2 activity from naja naja venom]. | | 1977 | 889937 |
| proton-nuclear-magnetic-resonance study of the conformation of neurotoxin ii from middle-asian cobra (naja naja oxiana) venom. | a proton nuclear magnetic resonance (nmr) study at 100 and 300 mhz of neurotoxin ii from the venom of middle-asian cobra naja naja oxiana has been performed in 2h2o and h2o solutions. by means of chemical modification and double resonance all the aromatic residue resonances have been assigned. from the nmr titration curves, pk values of histidine 4 and histidine 31 residues have been determined. for one of the two neighbouring tryptophan residues ph dependence (in the 2-8-ph range) of the chemic ... | 1976 | 12972 |
| the primary structure of toxin b from the venom of the indian cobra naja naja. | | 1976 | 992083 |
| [structural analysis of lethal snake venom neurotoxins by change transfer]. | among the two trp and the unique tyr present in the amino-acid sequence of the neurotoxin of naja naja philippinensis, only one trp is reactive in intermolecular charge transfer experiments, using organic electron acceptors. in the reference toxin-alpha of naja nigricollis, neither the single trp nor the tyr, both invariant and essential for lethality, are available for charge transfer. a minor and very weak complex is obtained, which is assumed to be initiated by an exposed his. although fully ... | 1976 | 825318 |
| photoaffinity labeling and quaternary structure of the acetylcholine receptor from torpedo californica. | membrane fragments from electric tissue of torpedo californica containing nicotinic acetylcholine receptor are composed of four different polypeptide chains with molecular weights of 40,000 (alpha), 48,000 (beta), 62,000 (gamma), and 66,000 (delta). the alpha and beta chains are still present in all and gamma and delta in some of the receptor preparations after triton x-100 extraction and purification by affinity chromatography. all components of the receptor react covalently with the photoaffin ... | 1976 | 1066671 |
| [effect of central asian cobra (naja naja oxiana) venom on the biosynthesis of nucleic acids and protein in animal cells]. | cobra poison at a dose of 4 microng/kg of body weight intensified the 3h-thimidine incorporation into dna of mice liver tissue; 20-40 microng/kg of the poison inhibited the incorporation. single intraperitoneal administration of high doses of cobra poison (50-150 microng/kg) decreased the incorporation of 14c-thimidine into dna 1.2-1.9-fold. little doses of the poison increased the incorporation of 14c-hydrolysate of chlorella protein into proteins of liver cells and the high doses inhibited thi ... | 2013 | 1027229 |
| [acetylcholinesterase of cobra venom. determination of concentration of active sites]. | o-nitrophenyl dimethylcarbamate and organophosphorus inhibitors o-n-propyl-p-nitrophenyl methylphosphonate, o-n-butyl-p-nitrophenyl methylphosphonate, o,o-diethyl-p-nitrophenyl phosphate, o-n-butyl-s-(beta-ethylmercaptoethyl) methylthiophosphonate, methysulphate of o,o-diethyl-s-(beta-phenyldimethylammoniumethly) thiophosphate were used in the titration of acetylcholinesterase active site concentratration in naja naja oxiana venom. no side reactions with the acetylcholinesterase molecule as well ... | 1976 | 1030638 |
| hydrolysis of phosphatidylcholine liposomes by phospholipases a2. effects of the local anesthetic dibucaine. | (1) dibucaine evokes a downward shift in the phase transition temperature of saturated phosphatidylcholines, while it also affects the pretransition. (2) the binding of dibucaine to phosphatidylcholine liposomes increases sharply when the lipid is transformed from the gel phase to the liquid-crystalline phase. (3) the activity of naja naja phospholipase a2 towards dimyristoyl phosphatidylcholine liposomes is either stimulated or inhibited by dibucaine, depending on whether the substrate is in th ... | 1976 | 1260059 |
| effects of some immunosuppressive procedures on myasthenia gravis. | clinical observations. a total of 53 mg patients have been treated with different immunosuppressive methods (alone or combined) with the following effects: thymectomy was performed in 38 patients. the improvement was excellent in 15, and moderate or uncertain in 20. in three patients severe long-lasting deterioration followed the operation. acth treatment (n=32): initial deterioration during the 5-7 days of heavy acth treatment (1000 iu) was followed by an improvement lasting on an average 4 mon ... | 1976 | 183592 |
| studies on the annual reproductive cycle of the female cobra, naja naja. seasonal variation in plasma inorganic ions. | | 1976 | 2437 |
| kinetic analysis of phospholipase a2 activity toward mixed micelles and its implications for the study of lipolytic enzymes. | a detailed kinetic scheme is proposed for the action of phospholipase a2 on mixed micelles of phospholipid and surfactant: see article. where e is the enzyme, a is the mixed micelle, and b is the phospholipid substrate in the mixed micelle. this scheme takes into account quantitatively the involvement of the lipid-water interface in the action of this enzyme toward substrate in macromolecular lipid complexes. the kinetic equation for this scheme is derived and four simplifying assumptions which ... | 1975 | 1194274 |
| characterization and physical properties of the major form of phospholipase a2 from cobra venom (naja naja naja) that has a molecular weight of 11,000. | the major form of phospholipase a2 from cobra venom (naja naja naja) was prepared in 30% yield and was homogeneous on polyacrylamide gel electrophoresis with and without sodium dodecyl sulfate and on sephadex g-100 chromatography. the monomer molecular weight is about 11,000 according to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. ultracentrifugation and molecular sieve techniques were employed to confirm the molecular weight and to demonstrate a concentration-dependent aggregatio ... | 1975 | 1194273 |
| sensitivity of cultured embryonic heart cells to cardiotoxin obtained from naja naja siamensis venom. | | 1975 | 1239834 |
| studies on the annual reproductive cycle of the female cobra, naja naja. i. seasonal variation in plasma cholesterol. | | 1975 | 241552 |
| the isolation and sequence determination of a cytotoxin from the venom of the middle-asian cobra naja naja oxiana. | | 1974 | 4435217 |
| investigation of the naja naja siamensis toxin binding site of the cholinergic receptor protein from torpedo electric tissue. | | 1974 | 4430347 |
| amino acid sequence of neurotoxin i from naja naja oxiana venom. | | 1974 | 4411932 |
| amino acid sequence of oxiana alpha, the main neurotoxin of the venom of naja naja oxiana. | | 1974 | 4850498 |
| chemical modification of the tryptophan residue in toxin b from the venom of the indian cobra. | | 1974 | 4208572 |
| the major lethal neurotoxin of the venom of naja naja philippinensis. purification, physical and chemical properties, partial amino acid sequence. | | 1974 | 4426734 |
| modification of the invariant tryptophan residue of two naja naja neurotoxins. | | 1973 | 4776457 |
| the affinity-labeling of partially purified acetylcholine receptor from electric tissue of electrophorus. | the receptor for acetylcholine was partially purified by affinity chromatography of an extract in triton x-100 of membrane fragments from electric tissue. the receptor was assayed, after its reduction with dithiothreitol, by reaction with the affinity-alkylating agent, [methyl-(3)h]4-(n-maleimido)-benzyltrimethylammonium iodide. alternative labeling procedures, one useful for routine assay of picomole quantities of receptor and the other for labeling larger quantities of receptor, are described. ... | 1973 | 4519650 |
| localization of the five disulfide bridges in toxin b from the venom of the indian cobra (naja naja). | | 1973 | 4767311 |
| phospholipase isoenzymes from naja naja venom. ii. phospholipase a and b activities. | | 1973 | 4762267 |
| amino acid sequence of neurotoxin ii from naja naja oxiana venom. | | 1973 | 4747603 |
| phospholipase isoenzymes from naja naja venom. i. purification and partial characterization. | | 1973 | 4202693 |
| kinetic dependence of phospholipase a 2 activity on the detergent triton x-100. | a kinetic analysis is presented for the dependence of one form of phospholipase a(2) from cobra (naja naja) venom on the presence of the nonionic detergent triton x-100 for its activity towards egg phosphatidylcholine and synthetic dipalmitoyl glycerophosphorylcholine as substrates. an automatic recording ph-stat apparatus was employed in order to continuously monitor enzyme activity. the results obtained in this study are interpreted in terms of a change in the physical state of the phospholipi ... | 1973 | 4698263 |
| isolation of the nicotinic acetylcholine receptor by biospecific chromatography on insolubilized naja naja neurotoxin. | | 1972 | 4646866 |
| localization of the four disulfide bridges in cytotoxin ii from the venom of the indian cobra (naja naja). | | 1972 | 4674343 |
| seasonal changes in the secretory activity of adrenal gland of the cobra (naja naja, linn.). | | 1972 | 4635455 |
| appearance of acetylcholine receptors during differentiation of a myogenic cell line. | acquisition of acetylcholine receptors during differentiation of a clonal myoblast cell line was monitored with a neurotoxin isolated from venom of the indian cobra naja naja. toxin bound specifically and reversibly to acetylcholine receptors of the differentiated cells. specificity of the binding reaction was assayed by measurement of the ability of various cholinergic agonists and antagonists to compete with neurotoxin for its binding site. the rate of toxin binding paralleled the rate of inac ... | 1972 | 4507600 |
| the amino acid sequence of cytotoxin ii from the venom of the indian cobra (naja naja). | | 1972 | 4340873 |
| irradiated cobra (naja naja philippinensis) venom. | | 1972 | 4539443 |
| isolation of the principal neurotoxins of naja naja subspecies from the asian mainland. | | 1972 | 5072086 |
| modification of amino groups in naja naja neurotoxins and the preparation of radioactive derivatives. | | 1972 | 4259996 |
| amino acid sequence of cytotoxin i from the venom of the indian cobra (naja naja). | | 1971 | 4332596 |
| amino acid sequence of toxin a from the venom of the indian cobra (naja naja). | | 1971 | 5125232 |
| isolation of the principal neurotoxins of two naja naja subspecies. | | 1971 | 5568672 |
| the n-terminal amino acid sequences of two basic cytotoxic proteins from the venom of the indian cobra. | | 1971 | 5564473 |
| seasonal changes in the histology of the adrenal gland of the cobra, naja naja. | | 1971 | 5542988 |
| purification and some properties of toxin a from the venom of the indian cobra (naja naja). | | 1971 | 5314268 |
| anesthetic deaths in cobras (naja naja and ophiophagus hannah) with methoxyflurane. | | 1970 | 5465566 |
| purification and some properties of toxin a from the venom of the indian cobra. | | 1970 | 5447860 |
| chemical composition of naja naja: extractable lipids. | | 1969 | 5358066 |
| seasonal changes in the in vitro production of testicular androgens by the cobra (naja naja linn). | | 1969 | 5817028 |
| seasonal changes in the thyroid gland in the male cobra, naja naja l. | | 1969 | 5798451 |
| seasonal variations in histology and corticosteroid synthesis in vitro in the adrenal of the cobra (naja naja). | | 1969 | 5775360 |
| isolation of phospholipase a isoenzymes from naja naja venom and their action on membrane-bound enzymes. | | 1968 | 5705754 |
| the effects of whole cobra venom (naja naja) and its fractions on the heart. | | 1967 | 6080378 |
| phospholipase a properties of several snake venom preparations. | the hydrolytic properties of the venoms of seven species of snakes,crotalus adamanteus, ancistrodon contortrix, naja naja, bothrops atrox, ophiophagus hannah, crotalus atrox andvipera russeli, were studied with purified lecithins and mixtures of lecithins of known fatty acid and class composition as substrates.the relative rates of hydrolysis of the fatty acids by the above venoms were studied by analysis of the products of the reaction at intervals during the course of the reaction. of the seve ... | 1966 | 17805626 |
| seasonal changes in the testis of the cobra, naja naja (linn). | | 1966 | 6007029 |
| the hydrolysis of unimolecular films of p32-labelled lecithin, phosphatidylethanolamine and phosphatidylinositol with phospholipase a (naja naja venom). | | 1966 | 4287839 |
| actions of whole and fractionated indian cobra (naja naja) venom on skeletal muscle. | | 1965 | 19108198 |
| glycoproteins as components of the lethal factors in cobra venom (naja naja). | | 1965 | 4285392 |
| fractionation of indian cobra venom by column chromatography. ii. carboxymethyl cellulose. | | 1964 | 14220722 |