| identification of species of brucella using fourier transform infrared spectroscopy. | fourier transform infrared spectroscopy (ftir) is a technique that has been used over the years in chemical analysis for the identification of substances and is one that may be applied to the characterisation of microorganisms. the marked tendency of brucella towards variation in the smooth rough phase, together with the laboriousness and risk involved in the methods used in their identification, make their classification difficult. we studied the type strains of the different species and biovar ... | 2003 | 14500003 |
| neurobrucellosis and a demonstration of its involvement in spinal roots via magnetic resonance imaging. | to present a neurobrucellosis case with spinal root involvement by means of magnetic resonance imaging (mri). | 2003 | 14504617 |
| anatomy of an exposure: a hospital lab's recovery of brucella melitensis. | | 2003 | 14524145 |
| cloning and expression of bartonella henselae sucb gene encoding an immunogenic dihydrolipoamide succinyltransferase homologous protein. | immunoscreening of a zap genomic library of bartonella henselae strain houston-1 expressed in escherichia coli resulted in the isolation of a clone containing 3.5 kb bamhi genomic dna fragment. this 3.5 kb dna fragment was found to contain a sequence of a gene encoding a protein with significant homology to the dihydrolipoamide succinyltransferase of brucella melitensis (sucb). subsequent cloning and dna sequence analysis revealed that the deduced amino acid sequence from the cloned gene showed ... | 2003 | 14524617 |
| [acute calculous cholecystitis associated with brucella melitensis]. | | 2003 | 14525714 |
| molecular characterization of brucella abortus chromosome ii recombination. | large-scale genomic rearrangements including inversions, deletions, and duplications are significant in bacterial evolution. the recently completed brucella melitensis 16m and brucella suis 1330 genomes have facilitated the investigation of such events in the brucella spp. suppressive subtractive hybridization (ssh) was employed in identifying genomic differences between b. melitensis 16m and brucella abortus 2308. analysis of 45 ssh clones revealed several deletions on chromosomes of b. abortus ... | 2003 | 14526025 |
| acute brucellosis presenting with erythema nodosum. | brucellosis is a common world-wide zoonotic disease. cutaneous manifestations are not specific and affect 1-14% of patients with brucellosis. we describe two cases of young males presenting with skin lesions of erythema nodosum on the anterior surface of the legs. histopathology of skin biopsy revealed septal panniculitis, but the positive cultures of blood or bone marrow for brucella melitensis established the diagnosis of brucellosis. | 2003 | 14561053 |
| human mammary abscess caused by brucella melitensis: a case report. | | 2003 | 14563232 |
| activation and proliferation of t lymphocyte subpopulations in patients with brucellosis. | t-cell proliferation is a standard method to evaluate cellular immune responses against intracellular infectious agents. the present study was undertaken to look for expression of an early activation marker (cd69) and proliferation using a nonradioactive method to evaluate cellular immune response against a salt-extractable antigen from brucella melitensis 16m (rcm-bm) in patients suffering from brucellosis. | 2003 | 14567397 |
| [evaluation of 2 hemoculture media for the isolation of brucella spp.]]. | the diagnostic efficiency of two hemoculture media for the detection of different species of brucella strains was evaluated. strains of brucella melitensis, brucella suis, brucella abortus, brucella ovis, and brucella abortus s19 were used. each strain was diluted in phosphate buffer saline (pbs) to obtain a concentration of 10(5) colony forming units/ml (cfu/ml). blood from goats, pigs, cattle, and sheep was mixed with the bacterial suspension to obtain a final concentration minor or equal to 1 ... | 2003 | 14587372 |
| ovarian abscess due to brucella melitensis. | the case of a 25-y-old woman with brucellar ovarian abscess is reported. cultures of blood, ascites and a pus specimen yielded brucella melitensis. the possibility of ovarian abscess being caused by brucella melitensis should be considered in countries where the infection is endemic. | 2003 | 14606619 |
| spontaneous bacterial peritonitis due to brucella infection. | brucella infection is a systemic disease, but the microorganism rarely causes infections in the gastrointestinal system such as hepatitis, cholecystitis, colitis and pancreatitis. spontaneous bacterial peritonitis due to brucella is extremely rare. herein, we report a case of cirrhosis complicated with nongranulomatous hepatitis and peritonitis, both due to brucella. a 63 year-old man with diabetes mellitus was admitted to hospital with complaints of weakness, backache, abdominal pain and abdomi ... | 2003 | 14614644 |
| brucella spondylitis and sacroiliitis in the general population in mumbai. | 1) to identify patients of brucella spondylitis and sacroiliitis amongst patients of 'infective spondylitis' and 'sacroiliitis', 2) to study clinical and radiographic features and outcome of brucella spondylitis. | 2003 | 14621032 |
| attenuated signature-tagged mutagenesis mutants of brucella melitensis identified during the acute phase of infection in mice. | for this study, we screened 1,152 signature-tagged mutagenesis mutants of brucella melitensis 16m in a mouse model of infection and found 36 of them to be attenuated in vivo. molecular characterization of transposon insertion sites showed that for four mutants, the affected genes were only present in rhizobiaceae. another mutant contained a disruption in a gene homologous to mosa, which is involved in rhizopine biosynthesis in some strains of rhizobium, suggesting that this sugar may be involved ... | 2003 | 14638795 |
| the effects of streptozotocin-induced diabetes on brucellosis of rats. | it is believed that an infection is more common and runs a more protracted course in people with diabetes. in clinical practice, it is important to be aware of these associations, as the prognosis is often dependent upon prompt recognition and appropriate treatment. to show the course of brucellosis in the diabetic state, a model of brucella melitensis infection was used in the setting of streptozotocin-induced diabetes in rat. b. melitensis infection proceeded more severely in diabetic rats and ... | 2003 | 14642313 |
| neglected case of osteoarticular brucella infection of the knee. | a 49-year-old farmer had a history of recurrent knee effusion for 20 years. he did not report undergoing any diagnostic or therapeutic procedures apart from repeated aspirations of the joint fluid. after the isolation of brucella melitensis from the joint fluid, computed tomography-guided bone biopsy was performed and histopathologic examination of the biopsy sample confirmed the diagnosis of chronic brucella osteomyelitis. arthroscopic synovectomy combined with antimicrobial therapy with doxycy ... | 2003 | 14652892 |
| pathways for phosphatidylcholine biosynthesis in bacteria. | phosphatidylcholine (pc) is the major membrane-forming phospholipid in eukaryotes with important structural and signalling functions. although many prokaryotes lack pc, it can be found in significant amounts in membranes of rather diverse bacteria. two pathways for pc biosynthesis are known in bacteria, the methylation pathway and the phosphatidylcholine synthase (pcs) pathway. in the methylation pathway, phosphatidylethanolamine is methylated three times to yield pc, in reactions catalysed by o ... | 2003 | 14663079 |
| the effect of levamisole combined with the classical treatment in chronic brucellosis. | levamisole is an immunopotenciator drug which is used as an antihelmintic drug as well as very effective remedy on cellular immunity compared with humoral immunity. a total 71 patients (37 men, 34 women) who referred to our department between march 1997 and december 2001, with a history of the disease for about 1 year, were diagnosed as having chronic brucellosis through those tests brucella serum agglutination test (sat), sat with coombs and sat with 2-mercaptoethanol. the patients were randoml ... | 2003 | 14690014 |
| popliteal artery pseudoaneurysm: a rare complication of brucellosis. | brucellosis is still a public health problem, particularly in developing countries. after the primary infection subsides, a chronic stage characterized by nonspecific manifestations can develop, during which it may not be possible to isolate brucella organisms and agglutination test titers may or may not be high. we present the case of a 49-year-old man who had only nonspecific symptoms and a 2-month history of a pulsatile painful swelling in his right popliteal region. he had no history of trau ... | 2004 | 14705176 |
| guillain-barré syndrome associated with acute neurobrucellosis. | we report the case of a 14-year-old girl with guillain-barré syndrome associated with brucellosis due to brucella melitensis. the diagnosis was established by the isolation of b. melitensis from her blood and by the determination of high levels of brucella aglutinins in her sera and cerebrospinal fluid. a combination of rifampin, co-trimoxazole and physical therapy resulted in complete healing within 30 days. antibrucellar treatment continued for 12 weeks. this case report suggests that brucello ... | 2003 | 14712899 |
| antibody reactivity to omp31 from brucella melitensis in human and animal infections by smooth and rough brucellae. | group 3 of outer membrane proteins (omps) of brucella includes omp25 and omp31, which share 34% identity. omp25 is highly conserved in brucella species, and omp31 is present in all brucella species, except brucella abortus. antibodies to brucella melitensis omp31 have been sought only in infected sheep, and western blotting of sera from infected sheep did not reveal anti-omp31 reactivity. we obtained recombinant purified omp31 (b. melitensis) and tested its recognition by sera from humans and an ... | 2004 | 14715555 |
| fever without apparent source on clinical examination. | episodes of acute illness associated with fever are common in children. less common but also diagnostically challenging are episodes of prolonged fever in children. this article reports recent literature that has focused on the epidemiology, pathophysiology, clinical and laboratory evaluation, and treatment of both types of episodes. | 2004 | 14758122 |
| bartholin's gland abscess caused by brucella melitensis. | we report herein a case of bartholin's gland abscess caused by brucella melitensis. clinical microbiology laboratory workers in areas where this disease is endemic should be familiar with the bacteriological features of this organism and consider the possibility of a brucellar etiology in a broad range of clinical settings. | 2004 | 14766890 |
| [immediate results of aureomycin therapy of human brucellosis due to brucella melitensis; 32 cases]. | | 1950 | 14785628 |
| the suppressive effect of antimicrobial drugs on brucella melitensis infection in mice. | | 1950 | 14794915 |
| failure to isolate brucella from prostatic tissue of individuals living in an endemic area. | | 1950 | 14797729 |
| [undulant fever due to brucella melitensis; clinical and epidemiological considerations]. | | 1950 | 14813880 |
| [a case of brucellosis of rabbit in france]. | | 1951 | 14829886 |
| [studies on melitine]. | | 1951 | 14829887 |
| [second case of brucella infection of rabbit in france]. | | 1951 | 14829902 |
| [blood sucking insects as mechanical vectors of brucella]. | | 1951 | 14837305 |
| [fatal acute glomerulonephritis from brucella melitensis]. | | 1951 | 14843879 |
| [action of aureomycin and dihydrostreptomycin in experimental brucella melitensis infection in mice]. | | 1950 | 14844771 |
| [appearance of brucellosis melitensis in saône-et-loire]. | | 1951 | 14845450 |
| brucella melitensis infection presenting as an arthritis of the hip joint. | | 1951 | 14845894 |
| [agglutination reaction of brucella melitensis by the cerebrospinal fluid in neurobrucellosis]. | | 1951 | 14857397 |
| studies in the pathology and pathogenesis of experimental brucellosis. i. a comparison of the pathogenicity of brucella abortus, brucella melitensis, and brucella suis for guinea pigs. | | 1951 | 14861464 |
| the viability of brucella melitensis in naturally infected cured hams. | | 1951 | 14875894 |
| [the escape of brucella melitensis meyer and shaw, from experimentally infected snails, h. (cryptomphalus) aspersa müller]. | | 1951 | 14921439 |
| the comparative effect of related chemical groups on the growth of three species of brucella. | | 1952 | 14924103 |
| studies in swine brucellosis. i. the pathogenesis of artificial brucella melitensis infection. | | 1952 | 14924113 |
| [combined vaccination of guinea pig with anaculture and living b. 112 against brucella melitensis infection]. | | 1951 | 14930476 |
| [hepatitis and cirrhosis due to brucella melitensis]. | | 1952 | 14945826 |
| [the local transfer of allergy to melitin]. | | 1952 | 14954590 |
| [an observed phenomenon during the local transfer of allergy to melitin]. | | 1952 | 14954606 |
| brucella endocarditis complicated with a mycotic aneurysm of the superior mesenteric artery: a case report. | brucella endocarditis, although a rare complication of brucellosis, is the main cause of death attributable to this disease. mycotic aneurysm is an important extracardiac complication of infective endocarditis. mycotic aneurysm of the superior mesenteric artery is extremely rare. in the present case, we report a patient with brucella melitensis endocarditis complicated with a superior mesenteric artery aneurysm. to our knowledge, this is the first such reported case. | 2004 | 14975571 |
| brucella pathogenesis, genes identified from random large-scale screens. | pathogenicity islands, specialized secretion systems, virulence plasmids, fimbriae, pili, adhesins, and toxins are all classical bacterial virulence factors. however, many of these factors, though widespread among bacterial pathogens, are not necessarily found among bacteria that colonize eukaryotic cells in a pathogenic/symbiotic relationship. bacteria that form these relationships have developed other strategies to infect and grow in their hosts. this is particularly true for brucella and othe ... | 2004 | 14979322 |
| use of an immunobinding test on nitrocellulose paper to diagnose caprine brucellosis. | | 2004 | 14989360 |
| protection against brucellosis in goats, five years after vaccination with reduced-dose brucella melitensis rev 1 vaccine. | the protection conferred by the reduced-dose rev 1 brucella melitensis vaccine in goats that had been immunized 5 years previously was evaluated. sixteen goats vaccinated 5 years before with rev 1 (1 x 10(5) cfu) and 5 non-vaccinated goats were challenged with b. melitensis 16m (4 x 10(5) cfu) using the conjunctival route. after giving birth or aborting, the goats were sacrificed and tissue samples were taken for bacteriological study. the challenge strain was recovered in 12%, of the animals fr ... | 2004 | 14998310 |
| real-time multiplex pcr assay for detection of brucella spp., b. abortus, and b. melitensis. | the identification of brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. here, we describe a real-time pcr assay for confirmation of presumptive brucella isolates. the assay was designed in a multiplex format that will allow the rapid identification of brucella spp., b. abortus, and b. melitensis in a single test. | 2004 | 15004098 |
| statistical procedures for calculating the residual virulence of brucella abortus strain 19 (s19) and brucella melitensis strain rev 1 vaccines in mice: theoretical basis and practical applications. | regular control of the biological quality of live brucella abortus strain 19 (s19) and b. melitensis strain rev 1 vaccines is essential for the successful management of ruminant brucellosis in affected countries. the reference procedures recommended by the oie (world organisation for animal health) and the european pharmacopoeia include the determination of residual virulence, expressed as the recovery time 50 (rt50), of the tested (problem) vaccine in a reference mouse model compared with the r ... | 2003 | 15005562 |
| a case of brucellar septic arthritis of the knee with a prolonged clinical course. | brucellosis is a systemic infectious disease with a broad spectrum of clinical manifestations. arthritis is frequently observed in its course and may be one of the main presenting clinical features of the disease. we report a case of brucellar monoarthritis of the knee with a prolonged clinical course despite efficient antibiotic treatment. | 2005 | 15007623 |
| different responses of macrophages to smooth and rough brucella spp.: relationship to virulence. | by comparing smooth wild-type brucella strains to their rough mutants, we show that the lipopolysaccharide (lps) o side chain of pathogenic brucella has a dramatic impact on macrophage activation. it favors the development of virulent brucella by preventing the synthesis of immune mediators, important for host defense. we conclude that this o chain property is firmly linked to brucella virulence. | 2004 | 15039375 |
| adherence of brucella to human epithelial cells and macrophages is mediated by sialic acid residues. | the basis for the interaction of brucella species with the surface of epithelial cells before migration in the host within polymorphonuclear leucocytes is largely unknown. here, we studied the ability of brucella abortus and brucella melitensis to adhere to cultured epithelial (hela and hep-2) cells and thp-1-derived macrophages, and to bind extracellular matrix proteins (ecm). the brucellae adhered to epithelial cells forming localized bacterial microcolonies on the cell surface, and this proce ... | 2004 | 15056214 |
| in vitro antimicrobial susceptibility of brucella species. | mic50 and mic90 values of doxycycline, rifampicin, ciprofloxacin, trimethoprim-sulphamethoxazole and ceftriaxone for 42 blood isolates of brucella species were determined using the etest. thirty-seven isolates were identified as b. melitensis and five as b. abortus. doxycycline had the lowest and rifampicin the highest mic50 values. four strains were non-susceptible to rifampicin, and one strain was resistant to trimethoprim-sulphamethoxazole. there is no significantly important resistance probl ... | 2004 | 15081093 |
| a case of mitral stenosis complicated with seronegative brucella endocarditis. | brucellosis is a multisystemic disease. the most common cause of death from the disease is endocarditis. the aortic valve is most commonly affected. the disease rarely involves the mitral valve. a 30 year-old woman presented with complaints of chills and fever up to 38 degrees c at night, fatigue, palpitations, and dyspnea for the previous 3 weeks. cardiac auscultation revealed a diastolic murmur in the mitral area. her temperature was 38.3 degrees c. on echocardiographic examination, the mitral ... | 2004 | 15090714 |
| an in vitro study on antimicrobial activity of propolis from mugla province of turkey. | antibacterial and antifungal activities of acetone and dimethyl sulfoxide (dmso) extracts of 45 different propolis samples from the mugla province of turkey were investigated. antimicrobial activity of propolis varied depending on propolis sample, dosage of propolis, and the extraction solvents for all test microorganisms. antimicrobial activity of all propolis samples increased with increasing dosage without reaching a plateau at the highest dosage tested. except for brucella melitensis, the dm ... | 2004 | 15117559 |
| use of the bactec myco/f lytic medium for detection of brucella melitensis bacteremia. | in a prospective volume-controlled study, the sensitivity of the bactec myco/f lytic medium was similar to that of the peds plus/f and plus aerobic/f vials for detecting brucella melitensis bacteremia, but the time to detection with the myco/f lytic medium (101.4 +/- 46.7 h) was significantly longer than that with the combined comparator media (65.5 +/- 18.9 h, p = 0.004). | 2004 | 15131190 |
| pathologic changes associated with brucellosis experimentally induced by aerosol exposure in rhesus macaques (macaca mulatta). | to develop an aerosol exposure method for induction of brucellosis in rhesus macaques (macaca mulatta). | 2004 | 15141886 |
| antibody response and antigen-specific gamma-interferon profiles of vaccinated and unvaccinated pregnant sheep experimentally infected with brucella melitensis. | it is well known that the immune response in sheep against brucella melitensis is subject to individual variation, depending on diverse factors. it bears asking whether these factors (e.g. clinical disease, active infection, state of previous immunity), when affecting a group, can cause variation in the performance of different diagnostic tests. to clarify some of the circumstances in which this immune response can vary, we examine the immune-response profile of sheep protected against the clini ... | 2004 | 15145500 |
| development of a western blotting assay to discriminate brucella spp. and yersinia enterocolitica o:9 infections in sheep. | outer membrane proteins (omps) of rev-1 strain of brucella melitensis were used in a western blotting assay for the serological diagnosis of brucellosis in ovine sera. fifty-four sheep sera were tested and divided into the following groups: group a) n. 9 samples from one sheep that had been experimentally infected with y. enterocolitica o:9; group b) n. 10 samples collected from sheep infected with brucella melitensis and 1 sample from a sheep vaccinated with the rev 1 strain; group c) n. 10 sam ... | 2004 | 15164626 |
| the louse-borne human pathogen bartonella quintana is a genomic derivative of the zoonotic agent bartonella henselae. | we present the complete genomes of two human pathogens, bartonella quintana (1,581,384 bp) and bartonella henselae (1,931,047 bp). the two pathogens maintain several similarities in being transmitted by insect vectors, using mammalian reservoirs, infecting similar cell types (endothelial cells and erythrocytes) and causing vasculoproliferative changes in immunocompromised hosts. a primary difference between the two pathogens is their reservoir ecology. whereas b. quintana is a specialist, using ... | 2004 | 15210978 |
| oral vaccination with brucella melitensis wr201 protects mice against intranasal challenge with virulent brucella melitensis 16m. | human brucellosis can be acquired from infected animal tissues by ingestion, inhalation, or contamination of conjunctiva or traumatized skin by infected animal products. in addition, brucella is recognized as a biowarfare threat agent. although a vaccine to protect humans from natural or deliberate infection could be useful, vaccines presently used in animals are unsuitable for human use. we tested orally administered live, attenuated, purine auxotrophic b. melitensis wr201 bacteria for their ab ... | 2004 | 15213148 |
| survey of the seroprevalence of brucellosis in ruminants in kosovo. | a cross-sectional survey of the seroprevalence of brucellosis in sheep, goats and cattle in kosovo was made in january 2001. a total of 12,000 serum samples, from 7941 cattle, 3548 sheep and 511 goats, were screened using the rose bengal test. doubtful and positive results were further tested with competitive and indirect elisas. the overall serological prevalences derived from the samples positive to all three tests, were 6.26 per cent (95 per cent confidence intervals [ci] 5.5 to 7.1 per cent) ... | 2004 | 15224594 |
| prevention of laboratory-acquired brucellosis. | brucellosis is one of the most common laboratory-acquired infections, mostly because aerosolization is a mechanism of transmission in this setting. we report an exposure to brucella melitensis that occurred in a large microbiology laboratory and describe the strategy chosen for antibiotic prophylaxis and serological follow-up of exposed workers. | 2004 | 15227634 |
| childhood brucellosis--a microbiological, epidemiological and clinical study. | a total of 5726 blood specimens (from children aged 14 years and younger) were studied for the serological evidence of brucellosis. ninety-three (1.6 per cent) showed diagnostic agglutinin titres with a geometric mean titre of 403 (sd +/- 547). forty-three (59.7 per cent) blood specimens yielded the growth of brucella melitensis. thirty-nine patients (41.93 per cent) were shepherds, who constituted the major occupational group affected in the present series. more than 60 per cent of the patients ... | 2004 | 15233191 |
| development and evaluation as vaccines in mice of brucella melitensis rev.1 single and double deletion mutants of the bp26 and omp31 genes coding for antigens of diagnostic significance in ovine brucellosis. | the live attenuated brucella melitensis rev.1 strain is considered the best vaccine available for the prophylaxis of brucellosis in sheep caused by either b. melitensis or brucella ovis. however, its application stimulates antibody responses in vaccinated animals indistinguishable by the current conventional serological tests from those observed in infected animals. the periplasmic protein bp26 and the outer membrane protein (omp) omp31 are immunodominant antigens in the serological responses of ... | 2004 | 15246618 |
| brucellosis as a cause of prolonged fever in an era of pasteurized milk. | | 2003 | 15260406 |
| comparative whole-genome hybridization reveals genomic islands in brucella species. | brucella species are responsible for brucellosis, a worldwide zoonotic disease causing abortion in domestic animals and malta fever in humans. based on host preference, the genus is divided into six species. brucella abortus, b. melitensis, and b. suis are pathogenic to humans, whereas b. ovis and b. neotomae are nonpathogenic to humans and b. canis human infections are rare. limited genome diversity exists among brucella species. comparison of brucella species whole genomes is, therefore, likel ... | 2004 | 15262941 |
| case report: brucellosis: a re-emerging disease in thailand. | brucellosis is a zoonotic disease prevalent in many countries, but it has been reported only once in thailand, 36 years ago. we describe here two consecutive cases of brucellosis in bangkok, thailand. both cases presented with prolonged fever and weight loss. blood cultures taken from 2 patients yielded brucella melitensis. the slide agglutination test of blood samples were also positive, with a titer of 1:64 for antibodies to brucella. the first patient responded to a combination of doxycycline ... | 2004 | 15272752 |
| [brucella myopericarditis]. | | 2004 | 15274859 |
| role of catalase in the virulence of brucella melitensis in pregnant goats. | an isogenic kate mutant derived from virulent brucella melitensis 16m displays hypersensitivity to hydrogen peroxide in disk sensitivity assays but retains the capacity to colonize pregnant goats and induce abortion. these experimental findings indicate that although the sole periplasmic catalase of brucella melitensis functions as an antioxidant, this enzyme does not play a critical role in virulence in the natural host. | 2004 | 15288933 |
| immunogenicity of recombinant omp31 from brucella melitensis in rams and serum bactericidal activity against b. ovis. | detergent-extracted recombinant omp31 (romp31 extract) from brucella melitensis produced in escherichia coli was previously identified as a protective immunogen against b. ovis in mice. in this study, we evaluated the immunogenicity of romp31extract in rams. this immunogen was emulsified in an oil adjuvant and administered three times with 4 and 8 weeks intervals. antibody response was measured in serum by whole b. ovis elisa. specific antibodies to purified romp31 (pet-omp31) were detected by w ... | 2004 | 15327795 |
| enhanced efficacy of recombinant brucella abortus rb51 vaccines against b. melitensis infection in mice. | brucella abortus strain rb51 is an attenuated rough strain, currently being used as the official live vaccine for bovine brucellosis in the usa and several other countries. in strain rb51, the wboa gene, encoding a glycosyltransferase required for the o-side chain synthesis, is disrupted by an is711 element. recently, we have demonstrated that strain rb51wboa, rb51 complemented with a functional wboa gene, remains rough but expresses low quantities of o-side chain in the cytoplasm. mice vaccinat ... | 2004 | 15327798 |
| an epidemiological profile of brucellosis in tabuk province, saudi arabia. | all 137 brucellosis cases occurring in tabuk province, saudi arabia in 1997 were studied retrospectively. brucella agglutination titre of > or = 1/80, or rising titre plus history of typical signs and symptoms were considered evidence of infection. the incidence rate was 34/100,000, mean age 33.8 +/- 13.9 years (range: 3-72 years) and male:female ratio 1.8:1. there were 63.5% of cases rurally resident, 58.4% kept animals at home or elsewhere, 27.0% worked with animals and/or on farms, and 88.3% ... | 2001 | 15332781 |
| community-acquired bacteraemia in a rural area: predominant bacterial species and antibiotic resistance. | the invasion of the bloodstream represents one of the most important sequelae of infection. this study was conducted over an 18-month period to determine the predominant bacterial agents of a community-acquired bacteraemia seen at health centres in a rural area of jordan, and their antibiotic susceptibilities. blood samples were collected and cultured from 215 patients who presented with fever and presumed diagnosis of a bacteraemia. isolates were identified and tested for antibiotic susceptibil ... | 2004 | 15358829 |
| unusual clinical presentations of brucellosis. | the purpose of this paper is to draw attention to atypical presentations of brucellosis. a prospective study identified 240 consecutive patients with brucellosis admitted to our department between december 1999 and july 2002. from these cases we present 11 patients with unusual clinical presentations. neurobrucellosis, peritonitis, pericarditis, pancytopenia, and uveitis were diagnosed in 2 patients each and 1 presented with epididymo-orchitis. | 2004 | 15370660 |
| dna polymorphism in the omp25/omp31 family of brucella spp.: identification of a 1.7-kb inversion in brucella cetaceae and of a 15.1-kb genomic island, absent from brucella ovis, related to the synthesis of smooth lipopolysaccharide. | five genes homologous to the well-known omp25 and omp31 genes, that code for two major brucella spp. outer membrane proteins (omps), have been detected in the genome of brucella melitensis 16m and brucella suis 1330. in this work we have determined the nucleotide sequence of these five genes, named omp31b, omp25b, omp25c, omp25d and omp22, in the six classical brucella species reference strains and in representative strains of the recently proposed species brucella cetaceae and brucella pinniped ... | 2004 | 15374004 |
| isolated intracranial hypertension: a rare presentation of neurobrucellosis. | brucella melitensis infection is endemic in the eastern and south-eastern anatolia regions of turkey. we report an unusual case of brucella meningitis presenting with bilateral papilla stasis, diplopia and absence of other neurological involvement. diagnosis was made by positive culture of brucella spp. with a bactec 9120 system with inoculation of the patient's cerebrospinal fluid (csf). this is the first report of isolation of brucella spp. from csf on a bactec 9120 system for diagnosis of men ... | 2004 | 15374008 |
| release of periplasmic proteins of brucella suis upon acidic shock involves the outer membrane protein omp25. | the survival and replication of brucella in macrophages is initially triggered by a low intraphagosomal ph. in order to identify proteins released by brucella during this early acidification step, we analyzed brucella suis conditioned medium at various ph levels. no significant proteins were released at ph 4.0 in minimal medium or citrate buffer, whereas in acetate buffer, b. suis released a substantial amount of soluble proteins. comparison of 13 n-terminal amino acid sequences determined by ed ... | 2004 | 15385468 |
| the ton system, an abc transporter, and a universally conserved gtpase are involved in iron utilization by brucella melitensis 16m. | brucella spp. are gram-negative intracellular facultative pathogens that are known to produce 2,3-dihydroxybenzoic acid (dhba), a catechol siderophore that is essential for full virulence in the natural host. the mechanism of dhba entry into brucella and other gram-negative bacteria is poorly understood. using mini-tn5kmcat mutagenesis, we created a transposon library of brucella melitensis 16m and isolated 32 mutants with a defect in iron acquisition or assimilation. three of these transposon m ... | 2004 | 15385478 |
| brucella melitensis endocarditis; report of a case. | | 1949 | 15393760 |
| [not available]. | | 1949 | 15393947 |
| [anaculture vaccination of the guinea pig against brucella melitensis]. | | 1950 | 15434987 |
| generation of the brucella melitensis orfeome version 1.1. | the bacteria of the brucella genus are responsible for a worldwide zoonosis called brucellosis. they belong to the alpha-proteobacteria group, as many other bacteria that live in close association with a eukaryotic host. importantly, the brucellae are mainly intracellular pathogens, and the molecular mechanisms of their virulence are still poorly understood. using the complete genome sequence of brucella melitensis, we generated a database of protein-coding open reading frames (orfs) and constru ... | 2004 | 15489343 |
| a case of intracranial abscess due to brucella melitensis. | | 2004 | 15494262 |
| laboratory-acquired brucellosis. | we report two laboratory-acquired brucella melitensis infections that were shown to be epidemiologically related. blood culture isolates were initially misidentified because of variable gram stain results, which led to misdiagnoses and subsequent laboratory exposures. notifying laboratory personnel who unknowingly processed cultures from brucellosis patients is an important preventive measure. | 2004 | 15504276 |
| modeling of seb-induced host gene expression to correlate in vitro to in vivo responses. | detection of exposure to biological threat agents has relied on ever more sensitive methods for pathogen identification, but that usually requires pathogen proliferation to dangerous, near untreatable levels. recent events have demonstrated that assessing exposure to a biological threat agent well in advance of onset of illness or at various stages post-exposure is invaluable among the diagnostic options. there is an urgent need for better diagnostic tools that will be sensitive, rapid, and unam ... | 2004 | 15522586 |
| 18fdg-pet for monitoring disease activity in an hiv-1 positive patient with disseminated chronic osteomyelitic brucellosis due to brucella melitensis. | | 2005 | 15549294 |
| brucellosis in the west bank, palestine. | to identify the risk factors for brucellosis in palestine, to estimate their association with the disease and to recommend appropriate prevention measures. | 2004 | 15573193 |
| indirect elisa for the detection of antibodies to brucella melitensis in sheep milk. | | 2004 | 15573789 |
| genetic bases of the rifampin resistance phenotype in brucella spp. | rifampin is one of the most potent and broad-spectrum antibiotics against bacterial pathogens. its bactericidal activity is due to its ability to bind to the beta subunit of the dna-dependent rna polymerase encoded by the rpob gene. mutations of the rpob gene have been characterized in rifampin-resistant (rif(r)) strains of escherichia coli and mycobacterium tuberculosis. the genetic bases of rif(r) in brucella spp. are still unknown. in the present study, the nucleotide sequences of the rpob ge ... | 2004 | 15583262 |
| the bartonella henselae sucb gene encodes a dihydrolipoamide succinyltransferase protein reactive with sera from patients with cat-scratch disease. | bartonella henselae is a recently recognized pathogenic bacterium associated with cat-scratch disease, bacillary angiomatosis and bacillary peliosis. a recombinant clone expressing an immunoreactive antigen of b. henselae was isolated by screening a genomic dna cosmid library by western blotting with sera pooled from patients positive for b. henselae igg antibodies by indirect immunofluorescence (ifa). the deduced amino acid sequence of the 43.7 kda encoded protein was found to be 76.3 % identic ... | 2004 | 15585501 |
| pancytopenia, a rare hematologic manifestation of brucellosis in children. | the records of 54 children with brucellosis were evaluated retrospectively. among them, eight patients (14.8%) with pancytopenia were identified in a 7-year period between 1996 and 2003. six of the eight patients with pancytopenia had brucella melitensis isolated from blood cultures, and all eight patients had brucella agglutination titers of at least 1:320. agglutination test titers did not correlate with the degree of pancytopenia. fever was the most common manifestation, followed by malaise, ... | 2004 | 15591899 |
| characteristics of b. melitensis versus b. abortus bacteraemias. | to determine the epidemiological and the clinical characteristics of bacteremic brucellosis. | 2005 | 15603839 |
| brucellar spine infection--four case reports. | brucellosis can be difficult to diagnose because of the nonspecific and variable clinical picture. this systemic disease is still an important public health problem in the mediterranean basin. these four cases of brucellar spine infection originated from rural areas around aydin, turkey. the systemic complaints of fever, profuse sweating, malaise, polyarthromyalgia, and weight loss indicated the final diagnosis of brucellosis and presumptive diagnoses were made based on agglutination testing for ... | 2004 | 15633472 |
| synergy tests by e test and checkerboard methods of antimicrobial combinations against brucella melitensis. | two different synergy testing methods, the checkerboard and the e test methods, were used to compare the in vitro efficacies of various antimicrobial combinations against 16 brucella melitensis strains isolated from blood cultures. the rate of agreement of the e test and checkerboard methods was found to be 55%. the most concordant results were found for the streptomycin-doxycycline combination in 12 (75%) tests, in which four strains showed synergistic activity by e test and antagonistic activi ... | 2005 | 15634962 |
| [development of immunoreagents for the diagnostics of intestinal yersiniosis by antigen-binding lymphocytes]. | to diagnose intestinal yersiniosis, the detection of antigen-binding lymphocytes (abl) with respect to y. enterocolitica o3, o5 and o9 is proposed. experiments on the immunization of rabbits with y. enterocolitica lps of these serovars revealed that immunoreagents, according to the data of cross antigen-dependent rosette formation and its inhibition, had species specificity (abl of rabbits immunized with y. enterocolitica did not interact with salmonella, shigella and y. pseudotuberculosis antig ... | 2004 | 15636146 |
| severe thrombotic microangiopathy associated with brucellosis: successful treatment with plasmapheresis. | brucellosis is a disease that may lead to changes in hematologic parameters such as anemia, neutropenia, and thrombocytopenia; however, thrombotic microangiopathy (tma) is a rare finding. severe tma may be associated with life-threatening hematologic, renal, and neurologic disorders. to prevent this mortality caused by brucellosis, prompt recognition of this complication and prompt therapy are essential. a patient with tma associated with brucella melitensis is presented who initially presented ... | 2005 | 15678281 |
| a multiplexed pcr-coupled liquid bead array for the simultaneous detection of four biothreat agents. | we have developed a 10-plexed pcr assay coupled to a 12-plexed liquid bead array to rapidly screen environmental samples for b. anthracis, y. pestis, f. tularensis, and b. melitensis. highly validated species-specific primer sets were used to simultaneously amplify multiple diagnostic regions unique to each individual pathogen. resolution of the mix of amplified products was achieved by pcr product hybridization to corresponding probe sequences, attached to unique sets of fluorescent beads. the ... | 2004 | 15680215 |