| fatal pyogranulomatous myocarditis in 10 boxer puppies. | over a period of 5 years, 10 pure-bred boxer puppies, 9-16 weeks old, were presented with a history of sudden death and were diagnosed with pyogranulomatous myocarditis. the myocarditis was characterized by a mixed infiltrate composed predominantly of neutrophils and macrophages. in our retrospective study, original case records and archived materials were examined. all dogs were positive for borrelia burgdorferi on immunohistochemistry (ihc). there was no evidence of infectious agents in formal ... | 2016 | 26965234 |
| demographic characteristics and infectious diseases of a population of american black bears in humboldt county, california. | american black bears (ursus americanus) are common, widely distributed, and broad-ranging omnivorous mammals in northern california forests. bears may be susceptible to pathogens infecting both domestic animals and humans. monitoring bear populations, particularly in changing ecosystems, is important to understanding ecological features that could affect bear population health and influence the likelihood that bears may cause adverse impacts on humans. in all, 321 bears were captured between may ... | 2015 | 25700042 |
| serosurvey for selected pathogens in free-ranging american black bears (ursus americanus) in maryland, usa. | american black bears (ursus americanus) in maryland, usa, live in forested areas in close proximity to humans and their domestic pets. from 1999 to 2011, we collected 84 serum samples from 63 black bears (18 males; 45 females) in five maryland counties and tested them for exposure to infectious, including zoonotic, pathogens. a large portion of the bears had antibody to canine distemper virus and toxoplasma gondii, many at high titers. prevalences of antibodies to zoonotic agents such as rabies ... | 2014 | 25075540 |
| health survey of free-ranging raccoons (procyon lotor) in central park, new york, new york, usa: implications for human and domestic animal health. | we conducted health assessments on 113 free-ranging raccoons ( procyon lotor ) in central park, new york city, us, in february 2010, september 2010, and november 2011 in conjunction with a trap-vaccinate-release program to control a raccoon rabies epizootic. five individuals were sampled at two time points for 118 raccoon examinations in total. we tested 13 of 13 and 8 of 13 euthanized raccoons for rabies and canine distemper virus (cdv), respectively, by antigen testing on brain tissue; all wer ... | 2017 | 28135131 |
| immunohistochemical evaluation of the effects of paraffin section storage on biomarker stability. | environmental stresses can alter immunoreactivity of biomarkers in stored tissue sections. the effect of temperature and lighting on 49 cellular or microbial antigens was evaluated in 4 serial paraffin sections, cut 12 months, 10 months, 8 months, 5 months, 3 months, 1 month, 3 days, and 1 day before immunohistochemistry. slides were stored at room temperature (rt) in the dark, at 4°c in the dark, at rt under fluorescent light, or at rt with windowpane exposure to sunlight. immunohistochemistry ... | 2014 | 23435571 |
| parasitology and serology of free-ranging coyotes (canis latrans) in north carolina, usa. | coyotes (canis latrans) have expanded recently into the eastern us and can serve as a source of pathogens to domestic dogs (canis lupus familiaris), livestock, and humans. we examined free-ranging coyotes from central north carolina, us, for selected parasites and prevalence of antibodies against viral and bacterial agents. we detected ticks on most (81%) coyotes, with amblyomma americanum detected on 83% of those with ticks. fifteen (47%) coyotes were positive for heartworms (dirofilaria immiti ... | 2015 | 25984773 |
| disease control through fertility control: secondary benefits of animal birth control in indian street dogs. | we sought to (1) survey sexually intact street dogs for a wide range of diseases in three cities in rajasthan, india and (2) evaluate links between the health of non-treated dogs and both the presence and duration of animal birth control (abc) programs. abc regimes sterilize and vaccinate stray dogs in an attempt to control their population and the spread of rabies. they are commonly suggested to improve the health of those dogs they serve, but here we provide evidence that these benefits also e ... | 2014 | 24239212 |
| prevalence of antibodies to selected viruses and parasites in introduced and endemic carnivores in western madagascar. | introduced animals impact endemic populations through predation, competition, and disease transmission. populations of endemic carnivores in madagascar are declining, and pathogens transmitted from introduced species may further endanger these unique species. we assessed the exposure of introduced and endemic carnivores to common viral and parasitic pathogens in two national parks of madagascar (kirindy mitea national park and ankarafantsika national park) and their neighboring villages. we also ... | 2016 | 27195685 |
| prevalence of antibodies for selected canine pathogens among wolves (canis lupus) from the alaska peninsula, usa. | we collected blood samples from wolves ( canis lupus ) on the alaska peninsula, southwest alaska, us, 2006-11 and tested sera for antibodies to canine adenovirus (cav), canine coronavirus (ccv), canine distemper virus (cdv), canine herpesvirus (chv), canine parainfluenza (cpi), canine parvovirus (cpv), neospora caninum , and toxoplasma gondii . detected antibody prevalence was 90% for cav, 28% for ccv, 12% for cdv, 93% for chv, 0% for cpi, 20% for cpv, 0% for n. caninum, and 86% for t. gondii . ... | 2016 | 27195683 |
| patterns of exposure of iberian wolves (canis lupus) to canine viruses in human-dominated landscapes. | wildlife inhabiting human-dominated landscapes is at risk of pathogen spill-over from domestic species. with the aim of gaining knowledge in the dynamics of viral infections in iberian wolves (canis lupus) living in anthropized landscapes of northern spain, we analysed between 2010 and 2013 the samples of 54 wolves by serology and polymerase chain reaction (pcr) for exposure to four pathogenic canine viruses: canine distemper virus (cdv), canine parvovirus-2 (cpv), canine adenovirus 1 and 2 (cav ... | 2016 | 26589403 |
| exposure to selected pathogens in to selected pathogens in geoffroy's cats and domestic carnivores from central argentina. | wild carnivores share a high percentage of parasites and viruses with closely related domestic carnivores. because of increased overlap and potential contact with domestic species, we conducted a retrospective serosurvey for 11 common carnivore pathogens in 40 geoffroy's cats (leopardus geoffroyi) sampled between 2000 and 2008 within or near two protected areas in central argentina (lihué calel national park, la pampa, and campos del tuyú national park, buenos aires), as well as five domestic ca ... | 2012 | 23060491 |
| enteropathogen infections in canine puppies: (co-)occurrence, clinical relevance and risk factors. | laboratory confirmation of the causative agent(s) of diarrhoea in puppies may allow for appropriate treatment. the presence of potential pathogens however, does not prove a causal relationship with diarrhoea. the aim of this study was to identify specific enteropathogens in ≤12 month old puppies with and without acute diarrhoea and to assess their associations with clinical signs, putative risk factors and pathogen co-occurrence. faecal samples from puppies with (n=113) and without (n=56) acute ... | 2016 | 27771056 |
| occurrence of canine parvovirus in dogs from henan province of china in 2009-2014. | there is no information concerning the genotype of canine parvovirus (cpv) currently circulating in henan province, china. therefore, the aim of the present study was to provide insights into the epidemiology and molecular characterization of cpv circulating in henan province from 2009 to 2014. | 2016 | 27377264 |
| prevalence and risk factors for viral exposure in rural dogs around protected areas of the atlantic forest. | despite the crucial role of domestic dogs as reservoirs for zoonosis and some of the most threatening diseases for wild carnivores such as distemper and parvovirosis, little is known about the epidemiological features and the risk factors involved in pathogen exposure of dogs that live in human/wildlife interfaces and actually contacts wildlife. through a cross-sectional serological approach and questionnaire survey, we assessed the prevalence along with individual and environment-associated ris ... | 2016 | 26822375 |
| co-circulation of canine coronavirus i and iia/b with high prevalence and genetic diversity in heilongjiang province, northeast china. | to trace the evolution of canine coronavirus (ccov), 201 stool samples from diarrheic dogs in northeast china were subjected to reverse transcription-polymerase chain reactions (rt-pcrs) targeting the partial m and s genes of ccov, followed by an epidemiological analysis. m gene rt-pcrs showed that 28.36% (57/201) of the samples were positive for ccov; of the 57 positive samples, ccov-i and ccov-ii accounted for 15.79% (9/57) and 84.21% (48/57), respectively. a sequence comparison of the partial ... | 2016 | 26771312 |
| co-circulation of the rare cpv-2c with unique gln370arg substitution, new cpv-2b with unique thr440ala substitution, and new cpv-2a with high prevalence and variation in heilongjiang province, northeast china. | to trace evolution of canine parvovirus-2 (cpv-2), a total of 201 stool samples were collected from dogs with diarrhea in heilongjiang province of northeast china from may 2014 to april 2015. the presence of cpv-2 in the samples was determined by pcr amplification of the vp2 gene (568 bp) of cpv-2. the results revealed that 95 samples (47.26%) were positive for cpv-2, and they showed 98.8%-100% nucleotide identity and 97.6%-100% amino acid identity. of 95 cpv-2-positive samples, types new2a (ser ... | 2015 | 26348721 |
| prevalence and phylogenetic analysis of canine kobuviruses in diarrhoetic dogs in northeast china. | canine kobuviruses (cakvs) are newly recognized picornaviruses that have been recently detected in dogs in the u.s.a., italy, u.k., the republic of korea and tanzania. to trace the evolution of cakv strains, a total of 201 fecal samples from rectal swabs of diarrheic dogs, which were obtained from may 2014 to april 2015 in northeast china, were detected by reverse transcription-pcr targeting a partial (504 bp) fragment of the 3d gene. furthermore, a phylogenetic analysis of the cakv strains iden ... | 2016 | 26256044 |
| establishment of reverse transcription loop-mediated isothermal amplification for rapid detection and differentiation of canine distemper virus infected and vaccinated animals. | although widespread vaccination against canine distemper virus (cdv) has been conducted for many decades, several canine distemper outbreaks in vaccinated animals have been reported frequently. in order to detect and differentiate the wild-type and vaccine strains of the cdv from the vaccinated animals, a novel reverse transcription loop-mediated isothermal amplification (rt-lamp) method was developed. a set of four primers-two internal and two external-were designed to target the h gene for the ... | 2015 | 25769803 |
| canine adenovirus type 1 in a fennec fox (vulpes zerda). | a 10-mo-old female fennec fox (vulpes zerda) with drooling suddenly died and was examined postmortem. histologic examination of different tissue samples was performed. vacuolar degeneration and diffuse fatty change were observed in the liver. several diagnostic methods were used to screen for canine parvovirus, canine distemper virus, canine influenza virus, canine coronavirus, canine parainfluenza virus, and canine adenovirus (cadv). only cadv type 1 (cadv-1) was detected in several organs (liv ... | 2014 | 25632689 |
| antiviral activity of a bacillus sp. p34 peptide against pathogenic viruses of domestic animals. | p34 is an antimicrobial peptide produced by a bacillus sp. strain isolated from the intestinal contents of a fish in the brazilian amazon basin with reported antibacterial activity. the aim of this work was to evaluate the peptide p34 for its in vitro antiviral properties against canine adenovirus type 2 (cav-2), canine coronavirus (ccov), canine distemper virus (cdv), canine parvovirus type 2 (cpv-2), equine arteritis virus (eav), equine influenza virus (eiv), feline calicivirus (fcv) and felin ... | 2014 | 25477947 |
| characterization of pantropic canine coronavirus from brazil. | characterization of canine coronavirus (ccov) strains currently in circulation is essential for understanding viral evolution. the aim of this study was to determine the presence of pantropic ccov type iia in tissue samples from five puppies that died in southern brazil as a result of severe gastroenteritis. reverse-transcriptase pcr was used to generate amplicons for sequence analysis. phylogenetic analysis of the ccov-iia strains indicated that they were similar to those found in other countri ... | 2014 | 25294661 |
| detection and genetic characterization of canine parvovirus and canine coronavirus strains circulating in district of tirana in albania. | an epidemiological survey for canine parvovirus 2 (cpv-2) and canine coronavirus (ccov) was conducted in albania. a total of 57 fecal samples were collected from diarrheic dogs in the district of tirana during 2011-2013. the molecular assays detected 53 and 31 cpv- and ccov-positive specimens, respectively, with mixed cpv-ccov infections diagnosed in 28 dogs. the most frequently detected cpv type was 2a, whereas iia was the predominant ccov subtype. a better comprehension of the cpv-ccov epidemi ... | 2014 | 24928599 |
| molecular and serological surveillance of canine enteric viruses in stray dogs from vila do maio, cape verde. | infections caused by canine parvovirus, canine distemper virus and canine coronavirus are an important cause of mortality and morbidity in dogs worldwide. prior to this study, no information was available concerning the incidence and prevalence of these viruses in cape verde archipelago. | 2014 | 24755118 |
| presence of infectious agents and co-infections in diarrheic dogs determined with a real-time polymerase chain reaction-based panel. | infectious diarrhea can be caused by bacteria, viruses, or protozoan organisms, or a combination of these. the identification of co-infections in dogs is important to determine the prognosis and to plan strategies for their treatment and prophylaxis. although many pathogens have been individually detected with real-time polymerase chain reaction (pcr), a comprehensive panel of agents that cause diarrhea in privately owned dogs has not yet been established. the objective of this study was to use ... | 2014 | 24433321 |
| clinical, hematological, and biochemical findings in puppies with coronavirus and parvovirus enteritis. | the clinical and laboratory findings in puppies naturally infected with canine coronavirus (ccov) and/or canine parvovirus (cpv) were compared with findings in uninfected puppies. lymphopenia was the only parameter related to ccov infection that was statistically significant; vomiting, anorexia, lethargy, hemorrhagic fluid diarrhea, leukopenia, lymphopenia, thrombocytopenia, hypoglycemia, and hypoproteinemia were correlated with cpv infection. | 2013 | 24155496 |
| mast cells in canine parvovirus-2-associated enteritis with crypt abscess. | the role of mast cells (mcs) in allergic reactions and parasitic infections is well established. their involvement in host immune response against bacterial and viral infections is reported. in this study, investigation is made to determine if mcs are associated with canine parvovirus-2 (cpv-2)-induced enteritis with crypt abscess (eca). mast cell count (mcc) was made on toluidine blue-stained intestinal sections from a total of 34 dogs. these included 16 dogs exhibiting eca positive for cpv-2 a ... | 2013 | 23558973 |
| pathogens of wild maned wolves (chrysocyon brachyurus) in brazil. | the maned wolf, chrysocyon brachyurus, is an endangered neotropical canid that survives at low population densities. diseases are a potential threat for its conservation but to date have been poorly studied. we performed clinical evaluations and investigated the presence of infectious diseases through serology and coprologic tests on maned wolves from galheiro natural private reserve, perdizes city, minas gerais state, southeastern brazil. fifteen wolves were captured between 2003 and 2008. we f ... | 2012 | 23060508 |
| enteropathogens identified in dogs entering a florida animal shelter with normal feces or diarrhea. | to determine the frequency of enteropathogens in dogs entering an animal shelter with normal feces or diarrhea. | 2012 | 22812470 |
| validation of a fecal scoring scale in puppies during the weaning period. | in puppies weaning is a high risk period. fecal changes are frequent and can be signs of infection by digestive pathogens (bacteria, viruses, parasites) and indicators of nutritional and environmental stress. the aim of this study was to define a pathological fecal score for weaning puppies, and to study the impact on that score of two intestinal viruses (canine parvovirus type 2 and canine coronavirus). for this, the quality of stools was evaluated on 154 puppies between 4 and 8 weeks of age (1 ... | 2012 | 22520179 |
| fatal outbreaks in dogs associated with pantropic canine coronavirus in france and belgium. | infection with pantropic canine coronavirus was detected during outbreaks in france and belgium. this was concurrent in most cases with canine parvovirus 2c. one outbreak was a deadly acute systemic disease with a single pantropic canine coronavirus infection. this is the first report of a fatality associated with pantropic canine coronavirus alone outside italy. | 2012 | 22320357 |
| polymerase spiral reaction (psr): a novel, visual isothermal amplification method for detection of canine parvovirus 2 genomic dna. | canine parvovirus-2 (cpv-2), which is ubiquitously distributed worldwide, causes severe and often fatal gastroenteritis in dogs. accurate, differential and rapid diagnosis of canine parvoviral enteritis remains a challenge for clinicians. a recently developed isothermal amplification technique, polymerase spiral reaction (psr), was optimized for the first time for a viral pathogen with reference recombinant plasmid standards from different cpv-2 antigenic variants (cpv-2, cpv-2a, cpv-2b and cpv- ... | 2017 | 28349355 |
| multiple-host pathogens in domestic hunting dogs in nicaragua's bosawás biosphere reserve. | nicaragua's bosawás biosphere reserve is a vast forested area inhabited largely by indigenous mayangna and miskitu people. most bosawás residents rely on subsistence hunting and swidden agriculture, and hunting dogs are important for finding and securing wild game. we investigated the health of hunting dogs in three communities differing in location, size, and economy. dogs in all communities were nutritionally compromised and experienced a heavy burden of disease. seroprevalence of canine diste ... | 2017 | 28012903 |
| the occurrence of pathogens in an endangered population of american badgers (taxidea taxus jacksoni) in ontario, canada. | american badgers ( taxidea taxus jacksoni) at the periphery of the species' range in ontario, canada, are listed as endangered because of an estimated population size of <200 mature individuals. the main threats faced by this population include habitat loss and road mortality. however, on 18 november 2013, a radio-implanted badger was found nonresponsive in an agricultural field with signs consistent with canine distemper virus infection, which was subsequently confirmed. this prompted our inves ... | 2017 | 27749192 |
| circovirus in domestic and wild carnivores: an important opportunistic agent? | circoviruses are relatively novel pathogens with increased importance in canids. in this study, we first screened the presence of dog circovirus (dogcv) by molecular methods from a total number of 389 internal organ samples originating from 277 individuals of domestic dogs and wild animals including wolves, foxes and badgers. all the animals originated from central-southern italy, specifically from abruzzi and molise regions, areas hosting several natural parks. dogcv was detected in 9/34 wolves ... | 2016 | 26848830 |
| dog overpopulation and burden of exposure to canine distemper virus and other pathogens on santa cruz island, galapagos. | dog overpopulation and diseases are hazards to native island species and humans on the galapagos. vaccination and importation of dogs are prohibited on the galapagos. risk management of these hazards requires the use of science-based risk assessment and risk communication. the objectives of the study reported here were (i) to estimate the human:dog ratio and (ii) the prevalence of and identify exposure factors associated with positive antibody titers to canine distemper virus (cdv) and other pat ... | 2016 | 26700426 |
| concomitance and interactions of pathogens in the iberian wolf (canis lupus). | with the aim of improving our understanding of their epidemiological features, exposure to or presence of canine parvovirus (cpv), canine distemper virus (cdv), leishmania infantum and sarcoptes scabiei were studied in 88 wild wolves from asturias (northern spain) by means of long-term (2004-2010) serological and molecular data. individual and population factors and the possible interactions between them were also statistically analyzed for better understanding the contact/presence of studied pa ... | 2015 | 26267084 |
| clinical and molecular investigation of a canine distemper outbreak and vector-borne infections in a group of rescue dogs imported from hungary to switzerland. | canine distemper virus (cdv) is a major pathogen of dogs and wild carnivores worldwide. in switzerland, distemper in domestic dogs is rarely reported. in recent years, the import of dogs from eastern europe to switzerland has steadily increased. in the present study, we describe a distemper outbreak in 15 rescue dogs that were imported from hungary to switzerland by an animal welfare organisation. the data on vaccination and medical history were recorded (14 dogs), and the samples were collected ... | 2015 | 26179635 |
| epidemiology of canine distemper and canine parvovirus in domestic dogs in urban and rural areas of the araucanía region in chile. | to assess whether the seroprevalence of canine distemper virus (cdv) and canine parvovirus (cpv) in domestic dogs is higher in urban versus rural areas of the araucanía region in chile and risk factors for exposure, a serosurvey and questionnaire survey at three, urban-rural paired sites was conducted from 2009 to 2012. overall, 1161 households were interviewed of which 71% were located in urban areas. a total of 501 blood samples were analysed. the overall cdv and cpv seroprevalences were 61% ( ... | 2015 | 26013417 |
| serological detection of infection with canine distemper virus, canine parvovirus and canine adenovirus in communal dogs from zimbabwe. | domestic dogs are common amongst communities in sub-saharan africa and may serve as important reservoirs for infectious agents that may cause diseases in wildlife. two agents of concern are canine parvovirus (cpv) and canine distemper virus (cdv), which may infect and cause disease in large carnivore species such as african wild dogs and african lions, respectively. the impact of domestic dogs and their diseases on wildlife conservation is increasing in zimbabwe, necessitating thorough assessmen ... | 2014 | 25686382 |
| serologic evidence for selected infectious diseases in marsican brown bears (ursus arctos marsicanus) in italy (2004-09). | we tested 30 serum samples collected during 2004-09 from 22 free-ranging marsican brown bears (ursus arctos marsicanus) in the national park of abruzzo, lazio, and molise, italy, for antibodies against canine distemper virus (cdv), canine adenovirus type 2 (cav-2), canine parvovirus type 2 (cpv-2), brucella spp., and eight leptospira interrogans sensu lato serovars. antibody to cdv was detected in 11 samples (37%); only two bears (10%) had detectable cav-2 and brucella spp. antibodies; three bea ... | 2015 | 25375945 |
| duration of immunity in red wolves (canis rufus) following vaccination with a modified live parvovirus and canine distemper vaccine. | there is growing information available regarding duration of immunity for core vaccines in both domestic and nondomestic species. vaccination protocols in nondomestic canids have frequently followed guidelines developed for the domestic dog; however, these protocols can be inappropriate for nondomestic canids such as the african wild dog (lycaon pictus), leaving some animals susceptible to infectious disease and others at risk for contracting vaccine-induced disease. in this study, red wolves (c ... | 2014 | 25314821 |
| preparation of monoclonal antibody of anti-feline calicivirus and establishment of double-antibody sandwich enzyme-linked immunosorbent assay detecting method. | this study aimed to prepare monoclonal antibody of feline calicivirus (fcv) and identify its basic biological characteristics. saturated ammonium sulfate precipitation, combined differential centrifugation, and cesium chloride density gradient centrifugation were used for the purification of fcv. the purified fcv was used as antigen to immunize balb/c mice. the hybridoma lines of anti-fcv monoclonal antibodies were established using cell fusion and hybridoma screening techniques. the subtypes of ... | 2014 | 25222237 |
| epidemiology of viral pathogens of free-ranging dogs and indian foxes in a human-dominated landscape in central india. | there is an increasing concern that free-ranging domestic dog (canis familiaris) populations may serve as reservoirs of pathogens which may be transmitted to wildlife. we documented the prevalence of antibodies to three viral pathogens, canine parvovirus (cpv), canine distemper virus (cdv) and canine adenovirus (cav), in free-ranging dog and sympatric indian fox (vulpes bengalensis) populations in and around the great indian bustard wildlife sanctuary, in maharashtra, central india. a total of 2 ... | 2014 | 25135467 |
| serologic survey for cross-species pathogens in urban coyotes (canis latrans), colorado, usa. | abstract as coyotes (canis latrans) adapt to living in urban environments, the opportunity for cross-species transmission of pathogens may increase. we investigated the prevalence of antibodies to pathogens that are either zoonotic or affect multiple animal species in urban coyotes in the denver metropolitan area, colorado, usa, in 2012. we assayed for antibodies to canine parvovirus-2, canine distemper virus, rabies virus, toxoplasma gondii, yersinia pestis, and serotypes of leptospira interrog ... | 2014 | 25121408 |
| parasitology, virology, and serology of free-ranging coyotes (canis latrans) from central georgia, usa. | we examined 31 free-ranging coyotes (canis latrans) from central georgia, usa, for select parasites and viral agents. sixteen coyotes had adult heartworms (dirofilaria immitis). serum samples from 27 animals revealed antibodies against canine parvovirus (100%), canine distemper virus (48%), canine adenovirus (37%), and trypanosoma cruzi (7%); none were detected against leishmania spp. twenty-two of 24 (92%) coyotes were positive for toxoplasma gondii. real-time pcr of feces revealed 32% of coyot ... | 2014 | 25098300 |
| canine kobuvirus infections in korean dogs. | to investigate canine kobuvirus (cakov) infection, fecal samples (n = 59) were collected from dogs with or without diarrhea (n = 21 and 38, respectively) in the republic of korea (rok) in 2012. cakov infection was detected in four diarrheic samples (19.0 %) and five non-diarrheic samples (13.2 %). all cakov-positive dogs with diarrhea were found to be infected in mixed infections with canine distemper virus and canine parvovirus or canine adenovirus. there was no significant difference in the pr ... | 2014 | 24906525 |
| selection of an aptamer against rabies virus: a new class of molecules with antiviral activity. | rabies is a fatal central nervous system (cns) disease caused by the neurotropic rabies virus (rabv). the therapeutic management of rabv infections is still problematic, and novel antiviral strategies are urgently required. we established the rvg-bhk-21 cell line, which expresses rabv glycoprotein on the cell surface, to select aptamers. through 28 iterative rounds of selection, single-stranded dna (ssdna) aptamers were generated by exponential enrichment (selex). a virus titer assay and a real- ... | 2014 | 24486485 |
| high prevalence of antibodies against canine adenovirus (cav) type 2 in domestic dog populations in south africa precludes the use of cav-based recombinant rabies vaccines. | rabies in dogs can be controlled through mass vaccination. oral vaccination of domestic dogs would be useful in the developing world, where greater vaccination coverage is needed especially in inaccessible areas or places with large numbers of free-roaming dogs. from this perspective, recent research has focused on development of new recombinant vaccines that can be administered orally in a bait to be used as adjunct for parenteral vaccination. one such candidate, a recombinant canine adenovirus ... | 2013 | 23867013 |
| rabies, canine distemper, and canine parvovirus exposure in large carnivore communities from two zambian ecosystems. | disease transmission within and among wild and domestic carnivores can have significant impacts on populations, particularly for threatened and endangered species. we used serology to evaluate potential exposure to rabies virus, canine distemper virus (cdv), and canine parvovirus (cpv) for populations of african lions (panthera leo), african wild dogs (lycaon pictus), and spotted hyenas (crocuta crocuta) in zambia's south luangwa national park (slnp) and liuwa plain national park (lpnp) as well ... | 2013 | 23805791 |
| assessing demographic and epidemiologic parameters of rural dog populations in india during mass vaccination campaigns. | mass vaccination of dogs is a mainstay for efforts to control rabies and other viral pathogens. the success of such programs is a function of the ability to vaccinate sufficient proportions of animals to develop herd immunity. however, fully assessing success in reaching target vaccination-levels and in understanding the outcome of mass vaccination efforts is hindered if insufficient information is available on the demographics of dog populations and the prevalence of the targeted pathogens. whi ... | 2013 | 23664490 |
| aptamers targeting rabies virus-infected cells inhibit viral replication both in vitro and in vivo. | rabies is an acute fatal encephalitis disease that affects many warm-blooded mammals. the causative agent of the disease is rabies virus (rabv). currently, no approved therapy is available once the clinical signs have appeared. aptamers, oligonucleotide ligands capable of binding a variety of molecular targets with high affinity and specificity, have recently emerged as promising therapeutic agents. in this study, sixteen high-affinity single-stranded dna (ssdna) aptamers were generated by cell- ... | 2013 | 23333291 |
| concomitant canine distemper, infectious canine hepatitis, canine parvoviral enteritis, canine infectious tracheobronchitis, and toxoplasmosis in a puppy. | the concomitant infections of canine distemper virus (cdv), canine adenovirus a types 1 (cadv-1) and 2 (cadv-2), canine parvovirus type 2 (cpv-2), and toxoplasma gondii are described in a 43-day-old mixed-breed puppy. clinically, there were convulsions and blindness with spontaneous death; 14 siblings of this puppy, born to a 10-month-old dam, which was seropositive (titer: 1,024) for t. gondii, also died. necropsy revealed unilateral corneal edema (blue eye), depletion of intestinal lymphoid ti ... | 2013 | 23293164 |
| duration of serological response to canine parvovirus-type 2, canine distemper virus, canine adenovirus type 1 and canine parainfluenza virus in client-owned dogs in australia. | to determine whether client-owned dogs in australia, last vaccinated with canvac(®) vaccines containing canine parvovirus-type 2 (cpv-2), canine distemper virus (cdv), canine adenovirus type 2 (cav-2) ± canine parainfluenza virus (cpiv) at least 18 months ago, were seropositive or responded serologically to revaccination. | 2012 | 23186088 |
| booster effect of canine distemper, canine parvovirus infection and infectious canine hepatitis combination vaccine in domesticated adult dogs. | domesticated adult dogs with antibody titer classified as below 'high' to one or more of canine distemper virus (cdv), canine parvovirus type-2 (cpv-2) and canine adenovirus type-1 (cadv-1) were then given an additional inoculation, and the effectiveness of this booster evaluated 2 months later. consequently, cdv and cadv-1 antibody titer experienced a significant increase, but the same effect was not observed in the antibody titer of cpv-2. these findings suggest that with additional inoculatio ... | 2012 | 22672137 |
| comparison of two assays for detection of antibodies against canine parvovirus and canine distemper virus in dogs admitted to a florida animal shelter. | to compare 2 assays for use in the identification of dogs with a protective antibody titer (pat) against canine parvovirus (cpv) and canine distemper virus (cdv). | 2012 | 22515628 |
| accuracy of a point-of-care elisa test kit for predicting the presence of protective canine parvovirus and canine distemper virus antibody concentrations in dogs. | canine parvovirus (cpv) and canine distemper virus (cdv) are highly infectious and often fatal diseases with worldwide distributions, and are important population management considerations in animal shelters. a point-of-care elisa test kit is available to detect serum antibodies to cpv and cdv, and presumptively to predict protective status. the aim of this study was to determine the diagnostic accuracy of the test compared to cpv hemagglutination inhibition titers and cdv serum neutralization t ... | 2012 | 22381707 |
| efficacy of feline anti-parvovirus antibodies in the treatment of canine parvovirus infection. | this prospective, randomised, placebo-controlled, double-blinded study aimed to evaluate efficacy of commercially available feline anti-parvovirus antibodies in dogs with canine parvovirus infection. | 2017 | 28369886 |
| the genetic evolution of canine parvovirus - a new perspective. | to trace the evolution process of cpv-2, all of the vp2 gene sequences of cpv-2 and fpv (from 1978 to 2015) from genbank were analyzed in this study. then, several new ideas regarding cpv-2 evolution were presented. first, the vp2 amino acid 555 and 375 positions of cpv-2 were first ruled out as a universal mutation site in cpv-2a and amino acid 101 position of fpv feature i or t instead of only i in existing rule. second, the recently confusing nomenclature of cpv-2 variants was substituted wit ... | 2017 | 28362831 |
| prevalence and molecular epidemiology of canine parvovirus 2 in diarrheic dogs in colombia, south america: a possible new cpv-2a is emerging? | since its identification in 1978, canine parvovirus type 2 (cpv-2) has been considered a pathogen of great importance in the canine population because it causes severe enteritis with high mortality rates in pups. cpv-2 is a virus belonging to the family parvoviridae. currently, there are three described antigenic variants (cpv-2a, cpv-2b, and cpv-2c). cpv-2c is an emerging virus that is seen as a global health hazard. the objective of this work was to confirm the presence of cpv-2 in dogs with a ... | 2017 | 28284623 |
| canine parvovirus post-vaccination shedding: interference with diagnostic assays and correlation with host immune status. | | 2017 | 28283075 |
| mt-pcr panel detection of canine parvovirus (cpv-2): vaccine and wild-type cpv-2 can be difficult to differentiate in canine diagnostic fecal samples. | canine parvovirus (cpv-2) remains an important cause of devastating enteritis in young dogs. it can be successfully prevented with live attenuated cpv-2 vaccines when given at the appropriate age and in the absence of maternal antibody interference. rapid diagnosis of parvoviral enteritis in young dogs is essential to ensuring suitable barrier nursing protocols within veterinary hospitals. the current diagnostic trend is to use multiplexed pcr panels to detect an array of pathogens commonly resp ... | 2017 | 28254505 |
| role of canine circovirus in dogs with acute haemorrhagic diarrhoea. | canine circovirus (caninecv) has been detected in some dogs with severe haemorrhagic diarrhoea, but its pathogenic role is unclear. this study evaluated a suspected association between the presence of caninecv and acute haemorrhagic diarrhoea syndrome (ahds) in dogs. the prevalence of caninecv in dogs with ahds was compared with that in healthy dogs and those infected with canine parvovirus (cpv). additionally, time to recovery and mortality rate were compared between caninecv-positive and canin ... | 2017 | 28242782 |
| antiviral activity of phosphorylated radix cyathulae officinalis polysaccharide against canine parvovirus in vitro. | phosphorylated radix cyathulae officinalis kuan polysaccharides (prcps) was prepared according to three-factors, ratio of stmp (%) and stpp (%), reaction time and reaction temperature, and three level l9(3(4)) orthogonal design. the antiviral activity of nine prcps (prcps1-9) was systematically evaluated by three methods pre-adding mode, mixed mode, and post-adding mode. cellular activity was tested by the cck-8 assay. the results showed that the optimal modification conditions were the ratio of ... | 2017 | 28238913 |
| expanding veterinary biosurveillance in washington, dc: the creation and utilization of an electronic-based online veterinary surveillance system. | to enhance the early detection of emerging infectious diseases and bioterrorism events using companion animal-based surveillance. | 2017 | 28237237 |
| a serosurvey of diseases of free-ranging gray wolves (canis lupus) in minnesota. | we tested serum samples from 387 free-ranging wolves ( canis lupus ) from 2007 to 2013 for exposure to eight canid pathogens to establish baseline data on disease prevalence and spatial distribution in minnesota's wolf population. we found high exposure to canine adenoviruses 1 and 2 (88% adults, 45% pups), canine parvovirus (82% adults, 24% pups), and lyme disease (76% adults, 39% pups). sixty-six percent of adults and 36% of pups exhibited exposure to the protozoan parasite neospora caninum . ... | 2017 | 28192048 |
| prevalence of increased canine pancreas-specific lipase concentrations in young dogs with parvovirus enteritis. | pancreatic abnormalities during canine parvovirus (cpv) enteritis have not been studied prospectively. | 2017 | 28125171 |
| molecular characterization of canine parvovirus in vientiane, laos. | the global emergence of canine parvovirus type 2c (cpv-2c) has been well documented. in the present study, 139 rectal swab samples collected from diarrheic dogs living in vientiane, laos, in 2016 were tested for the presence of the canine parvovirus (cpv) vp2 gene by pcr. the results showed that 82.73% (115/139) of dogs were cpv positive by pcr. the partial vp2 gene was sequenced in 94 of the positive samples; 91 samples belonged to cpv-2c (426glu) subtype, while 3 samples belonged to the cpv-2a ... | 2017 | 28124140 |
| faecal shedding of canine parvovirus after modified-live vaccination in healthy adult dogs. | since little is known about the persistence and faecal shedding of canine parvovirus (cpv) in dogs after modified-live vaccination, diagnostic tests for cpv can be difficult to interpret in the post-vaccination period. the primary aim of this study was to determine the incidence, duration and extent of cpv vaccine virus shedding in adult dogs and to investigate related factors, including the presence of protective antibodies, increase in anti-cpv antibody titres and development of any gastrointe ... | 2017 | 28093104 |
| novel chimeric virus-like particles vaccine displaying mers-cov receptor-binding domain induce specific humoral and cellular immune response in mice. | middle east respiratory syndrome coronavirus (mers-cov) has continued spreading since its emergence in 2012 with a mortality rate of 35.6%, and is a potential pandemic threat. prophylactics and therapies are urgently needed to address this public health problem. we report here the efficacy of a vaccine consisting of chimeric virus-like particles (vlp) expressing the receptor binding domain (rbd) of mers-cov. in this study, a fusion of the canine parvovirus (cpv) vp2 structural protein gene with ... | 2017 | 28040513 |
| multiplex amplification refractory mutation system pcr (arms-pcr) provides sequencing independent typing of canine parvovirus. | canine parvovirus-2 antigenic variants (cpv-2a, cpv-2b and cpv-2c) ubiquitously distributed worldwide in canine population causes severe fatal gastroenteritis. antigenic typing of cpv-2 remains a prime focus of research groups worldwide in understanding the disease epidemiology and virus evolution. the present study was thus envisioned to provide a simple sequencing independent, rapid, robust, specific, user-friendly technique for detecting and typing of presently circulating cpv-2 antigenic var ... | 2016 | 27989813 |
| palatability and clinical effects of an oral recuperation fluid during the recovery of dogs with suspected parvoviral enteritis. | dogs infected with canine parvovirus (cpv) can develop severe enteritis that requires supportive care until voluntary food and water consumption return. an oral recuperation fluid (orf) may assist in the overall recovery from cpv. the hypotheses of the study were that dogs with naturally infected cpv would prefer the orf to water and that dogs consuming the orf would have a more rapid return to voluntary appetite and improved caloric intake during the initial recovery period compared with dogs c ... | 2016 | 27968756 |
| full-length vp2 gene analysis of canine parvovirus reveals emergence of newer variants in india. | the canine parvovirus (cpv) infection is a highly contagious and serious enteric disease of dogs with high fatality rate. the present study was taken up to characterize the full-length viral polypeptide 2 (vp2) gene of cpv of indian origin along with the commercially available vaccines. the faecal samples from parvovirus suspected dogs were collected from various states of india for screening by pcr assay and 66.29% of samples were found positive. six cpv-2a, three cpv-2b, and one cpv-2c types w ... | 2016 | 27936872 |
| canine parvovirus (cpv-2) variants circulating in nigerian dogs. | canine parvovirus type 2 (cpv-2) is a highly contagious viral disease with three variants (cpv-2a, cpv-2b and cpv-2c) currently circulating in dogs worldwide. the main aim of this study was to determine the prevalent cpv-2 variant in faecal samples from 53 dogs presenting with acute gastroenteritis suspected to be and consistent with cpv-2 to nigerian veterinary clinics in 2013-2014. seventy-five per cent of these dogs tested positive for cpv-2 in a commercial antigen test and/or by pcr. partial ... | 2016 | 27933190 |
| genetic characterization of canine parvovirus type 2 subtypes in maputo, mozambique. | canine parvovirus type 2 (cpv-2) comprises three antigenic subtypes (2a, 2b and 2c) that have been reported in many countries. these subtypes cause serious disease in dogs with characteristic gastroenteritis signs. little information has been documented in africa about the genetic characterization of cpv-2. the aim of this study was to detect and to characterize the cpv-2 subtypes circulating in dogs admitted to veterinary clinics from two cities of mozambique, maputo and matola, in 2010. a tota ... | 2016 | 27888321 |
| multiplex amplification refractory mutation system pcr (arms-pcr) provides sequencing independent typing of canine parvovirus. | canine parvovirus-2 antigenic variants (cpv-2a, cpv-2b and cpv-2c) ubiquitously distributed worldwide in canine population causes severe fatal gastroenteritis. antigenic typing of cpv-2 remains a prime focus of research groups worldwide in understanding the disease epidemiology and virus evolution. the present study was thus envisioned to provide a simple sequencing independent, rapid, robust, specific, user-friendly technique for detecting and typing of presently circulating cpv-2 antigenic var ... | 2016 | 27876613 |
| natural and artificial hyperimmune solutions: impact on health in puppies. | colostrum and milk are complex mammary secretions providing the puppy with many nutritional and immunological factors, which play a crucial role for its correct development and survival. in the case of colostrum and/or milk intake deficiency, puppies are at increased risk of infectious diseases. this work reviews the various nutritional hyperimmune supplementations proposed to provide a passive immune protection and to positively impact puppies' health. some strategies rely on canine immunoglobu ... | 2016 | 27862411 |
| parvovirus capsid structures required for infection: mutations controlling receptor recognition and protease cleavages. | parvovirus capsids are small but complex molecular machines responsible for undertaking many of the steps of cell infection, genome packing, and cell-to-cell as well as host-to-host transfer. the details of parvovirus infection of cells are still not fully understood, but the processes must involve small changes in the capsid structure that allow the endocytosed virus to escape from the endosome, pass through the cell cytoplasm, and deliver the single-stranded dna (ssdna) genome to the nucleus, ... | 2017 | 27847360 |
| reliability of clinical diagnosis and laboratory testing techniques currently used for identification of canine parvovirus enteritis in clinical settings. | canine parvovirus type 2 (cpv-2) is the main etiological agent of viral enteritis in dogs. actually in literature, cpv-2 has been reported with clinical signs that vary from the classical disease, and immunochromatography test and pcr technique have been introduced to veterinary hospitals to confirm cpv-2 diagnosis and other infections. however, the reliability of these techniques has been poorly analyzed. in this study, we evaluated the sensitivity and specificity of veterinary clinical diagnos ... | 2017 | 27818461 |
| transcriptome profiling indicating canine parvovirus type 2a as a potential immune activator. | canine parvovirus type 2a (cpv-2a) is a variant of cpv-2, which is a highly contagious pathogen causing severe gastroenteritis and death in young dogs. however, how cpv-2 participates in cell regulation and immune response remains unknown. in this study, persistently infected mdck cells were generated through culture passage of the cpv-2a-infected cells for ten generations. our study showed that cpv-2a induces cell proliferation arrest and cell morphology alternation before the fourth generation ... | 2016 | 27339228 |
| poly (i:c) enhances the anti-tumor activity of canine parvovirus ns1 protein by inducing a potent anti-tumor immune response. | the canine parvovirus ns1 (cpv2.ns1) protein selectively induces apoptosis in the malignant cells. however, for an effective in vivo tumor treatment strategy, an oncolytic agent also needs to induce a potent anti-tumor immune response. in the present study, we used poly (i:c), a tlr3 ligand, as an adjuvant along with cpv2.ns1 to find out if the combination can enhance the oncolytic activity by inducing a potent anti-tumor immune response. the 4t1 mammary carcinoma cells were used to induce mamma ... | 2016 | 27209409 |
| direct typing of canine parvovirus (cpv) from infected dog faeces by rapid mini sequencing technique. | canine parvovirus (cpv) is a non-enveloped single stranded dna virus with an icosahedral capsid. mini-sequencing based cpv typing was developed earlier to detect and differentiate all the cpv types and fpv in a single reaction. this technique was further evaluated in the present study by performing the mini-sequencing directly from fecal samples which avoided tedious virus isolation steps by cell culture system. fecal swab samples were collected from 84 dogs with enteritis symptoms, suggestive o ... | 2016 | 27659245 |
| first detection of canine parvovirus type 2b from diarrheic dogs in himachal pradesh. | the present study was conducted to detect the presence of canine parvovirus (cpv) among diarrheic dogs in himachal pradesh and to identify the most prevalent antigenic variant of cpv based on molecular typing and sequence analysis of vp2 gene. | 2016 | 27733797 |
| seroprevalence of canine parvovirus in dogs in lusaka district, zambia. | canine parvovirus (cpv) enteritis is a highly contagious enteric disease of young dogs. limited studies have been done in zambia to investigate the prevalence of cpv in dogs. blood was collected from dogs from three veterinary clinics (clinic samples, n = 174) and one township of lusaka (field samples, n = 56). each dog's age, sex, breed, and vaccination status were recorded. a haemagglutination assay using pig erythrocytes and modified live parvovirus vaccine as the antigen was used. antibodies ... | 2016 | 27699205 |
| identification of a novel canine parvovirus type 2c in taiwan. | taiwan has been considered free from canine parvovirus type 2c (cpv-2c) based on the last report of canine parvovirus type 2 (cpv-2) surveillance. however, since january 2015, the first report of cpv-2c in a puppy has occurred in taiwan. there is currently limited information about the cpv-2c variant in taiwan. in the present study, we characterized the previously unidentified cpv-2c variant and investigated the distribution of cpv-2 variants in taiwan. | 2016 | 27663840 |
| near-atomic resolution structure of a highly neutralizing fab bound to canine parvovirus. | canine parvovirus (cpv) is a highly contagious pathogen that causes severe disease in dogs and wildlife. previously, a panel of neutralizing monoclonal antibodies (mab) raised against cpv was characterized. an antibody fragment (fab) of mab e was found to neutralize the virus at low molar ratios. using recent advances in cryo-electron microscopy (cryo-em), we determined the structure of cpv in complex with fab e to 4.1 å resolution, which allowed de novo building of the fab structure. the footpr ... | 2016 | 27535057 |
| molecular characterisation of parvoviruses from domestic cats reveals emergence of newer variants in india. | the present study was undertaken to characterise the viral polypeptide 2 (vp2) gene of parvovirus from domestic cats in india. | 2016 | 27502089 |
| two potential recombinant rabies vaccines expressing canine parvovirus virion protein 2 induce immunogenicity to canine parvovirus and rabies virus. | both rabies virus (rabv) and canine parvovirus (cpv) cause lethal diseases in dogs. in this study, both high egg passage flury (hep-flury) strains of rabv and recombinant rabv carrying double rabv glycoprotein (g) gene were used to express the cpv virion protein 2 (vp2) gene, and were designated rhep-vp2 and, rhep-dg-vp2 respectively. the two recombinant rabvs maintained optimal virus titration according to their viral growth kinetics assay compared with the parental strain hep-flury. western bl ... | 2016 | 27449079 |
| profiling of host cell response to successive canine parvovirus infection based on kinetic proteomic change identification. | canine parvovirus (cpv) reproduces by co-opting the resources of host cells, inevitably causing cytotoxic effects to the host cells. feline kidney f81 cells are sensitive to cpv infection and show disparate growing statuses at different time points post-infection. this study analysed the response of f81 cells to cpv infection at successive infection time points by itraq-based quantitative proteomics. differentially expressed proteins (deps) during 60 h of infection and at selected time points po ... | 2016 | 27406444 |
| canine parvovirus study. | | 2016 | 27389753 |
| canine parvovirus: the worldwide occurrence of antigenic variants. | the most important enteric virus infecting canids is canine parvovirus type 2 (cpv-2). cpv is the aetiologic agent of a contagious disease, mainly characterized by clinical gastroenteritis signs in younger dogs. cpv-2 emerged as a new virus in the late 1970s, which could infect domestic dogs, and became distributed in the global dog population within 2 years. a few years later, the virus's original type was replaced by a new genetic and antigenic variant, called cpv-2a. around 1984 and 2000, vir ... | 2016 | 27389721 |
| influence of breed size, age, fecal quality, and enteropathogen shedding on fecal calprotectin and immunoglobulin a concentrations in puppies during the weaning period. | fecal calprotectin and immunoglobulin a (iga) are markers of intestinal inflammation and immunity in adult dogs. | 2016 | 27279352 |
| scientific rebuttal to a published article "multiplex real-time pcr for identification of canine parvovirus antigenic types". | | 2016 | 27185565 |
| canine circovirus 1 (cacv-1) and canine parvovirus 2 (cpv-2): recurrent dual infections in a papillon breeding colony. | recurrent outbreaks of sudden death and bloody diarrhea were reported in march 2013 and february 2014 in a breeding colony of papillon dogs. during the first outbreak, 1 adult dog and 2 eight-month-old puppies died. during the second outbreak, 2 ten-week-old puppies died. one puppy from the first outbreak and 2 puppies from the second outbreak were examined at necropsy. histologically, all 3 puppies had severe segmental crypt necrosis of the small intestine and marked lymphoid follicle depletion ... | 2016 | 27154544 |
| phylogenetic characterization of canine parvovirus vp2 partial sequences from symptomatic dogs samples. | the aim of the present study was to detect canine parvovirus (cpv) from faecal samples of clinically ill domestic dogs by polymerase chain reaction (pcr) followed by vp2 gene partial sequencing and molecular characterization of circulating strains in lithuania. eleven clinically and antigen-tested positive dog faecal samples, collected during the period of 2014-2015, were investigated by using pcr. the phylogenetic investigations indicated that the lithuanian cpv vp2 partial sequences (3025-3706 ... | 2016 | 27096803 |
| molecular epidemiology of canine parvovirus in morocco. | since it first emergence in the mid-1970's, canine parvovirus 2 (cpv-2) has evolved giving rise to new antigenic variants termed cpv-2a, cpv-2b and cpv-2c, which have completely replaced the original strain and had been variously distributed worldwide. in africa limited data are available on epidemiological prevalence of these new types. hence, the aim of the present study was to determine circulating variants in morocco. through taqman-based real-time pcr assay, 91 samples, collected from sympt ... | 2016 | 27083072 |
| utility of a rapid immunochromatographic strip test in detecting canine parvovirus infection compared with polymerase chain reaction. | the present study was undertaken to detect the presence of canine parvovirus (cpv) in fecal samples of diarrheic dogs by conventional polymerase chain reaction (pcr) and immunochromatographic (ic) strip test and to compare the diagnostic potential of these tests. | 2015 | 27047127 |
| full-length genomic characterization and molecular evolution of canine parvovirus in china. | canine parvovirus type 2 (cpv-2) can cause acute haemorrhagic enteritis in dogs and myocarditis in puppies. this disease has become one of the most serious infectious diseases of dogs. during 2014 in china, there were many cases of acute infectious diarrhoea in dogs. some faecal samples were negative for the cpv-2 antigen based on a colloidal gold test strip but were positive based on pcr, and a viral strain was isolated from one such sample. the cytopathic effect on susceptible cells and the re ... | 2016 | 27038801 |
| [application of paramunity inducers in small animal practice]. | paramunity inducers have been used to treat small animals for decades. paramunity inducers are based on attenuated and inactivated poxviruses (avipox virus and parapox virus). their applications include both therapeutic and prophylactic use in various diseases. despite their wide and variable use, only a very small number of placebo-controlled studies has been published. positive effects in preventing kitten mortality and in treating feline stomatitis have been reported, however, no statisticall ... | 2016 | 26998822 |
| magnetic resonance imaging revealed splenic targeting of canine parvovirus capsid protein vp2. | canine parvovirus (cpv) is a highly contagious infectious virus, whose infectious mechanism remains unclear because of acute gastroenteritis and the lack of an efficient tool to visualize the virus in real time during virology research. in this study, we developed an iron oxide nanoparticle supported by graphene quantum dots (gqd), namely, fegqd. in this composite material, gqd acts as a stabilizer; thus, vacancies are retained on the surface for further physical adsorption of the cpv vp2 protei ... | 2016 | 26996514 |
| multiplex real-time pcr for identification of canine parvovirus antigenic types. | canine parvovirus (cpv) is an important disease causing gastroenteritis and/or haemorrhagic gastroenteritis in dogs. there are four antigenic types of cpv reported worldwide viz. cpv 2, cpv 2a, cpv 2b and cpv 2c. the diagnosis of cpv with the identification of the antigen type responsible remains problematic. in the present study, identification as well as antigenic typing of cpv was done using a de novo multiplex real time pcr to combat the problem of antigenic type identification. from the stu ... | 2016 | 26987970 |