| role of the adenovirus e1b 19,000-dalton tumor antigen in regulating early gene expression. | mutations in the adenovirus gene encoding the e1b 19-kilodalton protein (the 19k protein) result in pleiotropic phenotypes that affect the host cell and virus growth. examination of viral gene expression in hela cells infected with e1b 19k mutant viruses revealed synthesis and accumulation of e1a proteins to higher steady-state levels than those proteins synthesized during infection with the wild-type virus. as a consequence of elevated e1a levels, another early gene product, the 72k dna-binding ... | 1988 | 2969984 |
| differential distribution of the adenovirus e1a proteins and colocalization of e1a with the 70-kilodalton cellular heat shock protein in infected cells. | five distinct localization patterns were observed for the adenovirus e1a proteins in the nuclei of infected hela cells: diffuse, reticular, nucleolar, punctate, and peripheral. the variable distribution of e1a was correlated with the time postinfection and the cell cycle stage of the host cell at the time of infection. all staining patterns, with the exception of peripheral e1a localization, were associated with the early phase of infection since only the diffuse, reticular, nucleolar, and punct ... | 1988 | 2971821 |
| adenovirus infections in patients undergoing bone-marrow transplantation. | viral infection is commonly observed after bone-marrow transplantation. we isolated adenovirus from 51 of 1051 patients undergoing marrow transplantation between 1976 and 1982. of the 46 isolates available for typing, 13 (27.7 per cent) were of the closely related species 11, 34, or 35 (subgenus b). all 13 of the patients with these species had positive urine cultures. the species have previously been associated with the acquired immunodeficiency syndrome or with renal transplantation but are no ... | 1985 | 2982098 |
| a trans-acting factor is responsible for the simian virus 40 enhancer activity in vitro. | stimulation of in vitro transcription by the simian virus 40 enhancer involves a rapid and stable binding of a trans-acting factor with both the 5'- and 3'-domains of the enhancer sequence. the enhancer factor, which differs from other types of transcriptional factors, can interact with other enhancer elements. | 1985 | 2982105 |
| transfer of genes into hematopoietic cells using recombinant dna viruses. | the ability of recombinant dna viruses to transfer genes into hematopoietic cells has been explored. a recombinant simian virus 40 (sv40) in which the early region had been replaced with the chloramphenicol acetyltransferase (cat) gene driven by the promoter from rous sarcoma virus (rsv), was constructed. this virus transferred the cat gene more efficiently into mouse and human bone marrow cells and into the k562, mel, and wehi hematopoietic tissue culture cell lines, than the classical calcium ... | 1985 | 2982141 |
| adenovirus e1a proteins repress transcription from the sv40 early promoter. | using a transient expression assay in hela cells, we show that products from the adenovirus-5 e1a transcription unit repress transcription from the sv40 early promoter. the repression is unrelated to t antigen autoregulation, occurs maximally with low concentrations of e1a expression plasmid, is exerted at the transcriptional level, and requires functional e1a protein. the 289 and 243 amino acid e1a proteins are equally effective at repressing transcription. since only the 289 amino acid protein ... | 1985 | 2982503 |
| resistance to adenovirus infection after administration of bordetella pertussis vaccine in mice. | treatment of mice with bordetella pertussis vaccine rendered mice resistant to mouse adenovirus infection. the resistant state took at least 5 days to develop, and susceptibility returned to a portion of the test population 35 days after treatment. transient resistance developed in congenitally athymic mice also. treatment with a dose of 25 micrograms (dry weight) of b. pertussis vaccine protected approximately 50% of the test population. vaccines prepared from several different strains of b. pe ... | 1985 | 2982736 |
| simplified restriction endonuclease method for typing and subtyping adenoviruses. | restriction endonuclease digestion of viral dna labelled in vivo with phosphorus-32 has been used to type and to subtype both conventional and enteric adenoviruses. the method is a modification of that already described for typing and subtyping herpes simplex virus and, like the latter, needs far less material than methods previously described. | 1985 | 2982921 |
| adenoviruses and pediatric gastroenteritis. | adenoviruses were found in 8.6% of 900 pediatric inpatients with diarrhea who were tested by electron microscopy of a fecal specimen and cell culture inoculation of a throat swab and an anal swab specimen. in 5.1% of these patients, including 13.5% of patients who were four through five months of age, adenovirus particles were visualized in the fecal specimen. controlled study demonstrated that visualized adenoviruses, especially those that did not grow readily in conventional hep-2 cell culture ... | 1985 | 2982960 |
| identification of respiratory virus antigens in middle ear fluids of children with acute otitis media. | antigens of respiratory viruses were tested by immunoassay of the middle ear fluids and nasopharyngeal secretions of 137 children with acute otitis media. the following were found: (1) an epidemic of infection with respiratory syncytial virus (rsv) caused a significant increase in the occurrence of acute otitis media. (2) fifteen percent of the children had rsv antigens in middle ear fluid, and in 7% rsv was the sole pathogen found. adenovirus antigens were found in the middle ear fluid of 3% of ... | 1985 | 2982961 |
| [polyvinylpyrrolidone iodine and arginase: effect on corneal regeneration and its antiviral effect]. | pvp-iodine was found to be virucidal for adenoviruses and herpes simplex virus in a concentration of 0.5%, i.e., a 1:20 dilution of betaisodona. similar concentrations did not delay the healing of corneal epithelium of the rabbit after experimental lesions (ullerich and durchschlag, 1953), although the eyes were slightly irritated. however, a higher concentration (1.25%) caused considerable ocular irritation, and healing of the cornea was delayed or completely inhibited. arginase, which inhibite ... | 1985 | 2983142 |
| identification of the components necessary for adenovirus translational control and their utilization in cdna expression vectors. | a transient expression system was used to study the role of the adenovirus late and simian virus 40 (sv40) early mrna leader sequences and adenovirus virus-associated (va) rnas in mrna translation. hybrid transcription units containing the adenovirus late and sv40 early promoters fused to various coding regions were introduced into monkey cos cells on plasmids containing a sv40 origin of replication. the translational efficiencies of the mrnas produced from these plasmids were determined after a ... | 1985 | 2983309 |
| an adenovirus vector system used to express polyoma virus tumor antigens. | we have used a generalized adenovirus vector system to express the three polyoma tumor (t) antigen proteins under the control of the adenovirus major late promoter. one hybrid virus, ad-pysvr498, expresses high levels of polyoma middle and small t antigens. a second hybrid virus, ad-ltsvr545, which contains a cdna copy of the polyoma a gene, overproduces large t antigen. the t antigens produced are indistinguishable from their authentic polyoma counterparts as determined by immunoprecipitation a ... | 1985 | 2983341 |
| adenovirus-like particles associated with intranuclear inclusion bodies in the kidney of a common murre (uria aalge). | adenovirus-like particles were identified by transmission electron microscopy in intranuclear inclusion bodies in the renal collecting tubules of a male common murre. the bird had been trapped in an oil-spill and had been cleaned and held in captivity before euthanasia. the presence of the virus appeared to be causing little or no renal disease. it is thought that this bird suffered activation of a latent viral infection as a result of the stress of oil intoxication, handling, and confinement. | 1985 | 2985038 |
| partial sequence homologies between cytoskeletal proteins, c-myc, rous sarcoma virus and adenovirus proteins, transducin, and beta- and gamma-crystallins. | computer based sequence comparisons indicate partial sequence homology between human c-myc, rous sarcoma virus, adenovirus 7, and simian sarcoma virus proteins and the cytoskeletal proteins d9smin, keratin and vimentin. in addition, sections of the oncogene proteins showed partial but significant homology to alpha and gamma subunits of transducin. gamma-ii and beta-bp crystallins showed partial but significant homology to the cytoskeletal proteins keratin, vimentin, desmin, alpha and beta-tubuli ... | 1985 | 2985144 |
| transcription control by oncogenes. | | 1985 | 2986847 |
| dna sequence of the leftward junction in the adenovirus-simian virus 40 hybrid ad2+d2 and determination of the structure of the d2-t antigen. | the nucleotide sequence of the junction between the simian virus 40 early region and the adenovirus type 2 late region l4 in the hybrid virus ad2+d2 was determined. the deduced amino acid sequence suggests that the d2-t antigen is a chimeric protein sharing 594 amino acids with the c-terminal end of the simian virus 40 t antigen and 104 amino acids with the n terminus of the adenovirus type 2 33,000-molecular-weight protein. the predicted structure of the d2-t antigen was confirmed by an immunop ... | 1985 | 2987542 |
| fulminant liver failure induced by adenovirus after bone marrow transplantation. | | 1985 | 2987693 |
| overproduction of the protein product of a nonselected foreign gene carried by an adenovirus vector. | we have constructed a recombinant adenovirus that carries the herpes simplex virus type i gene for thymidine kinase (ec 2.7.1.21) and expresses thymidine kinase under control of adenovirus major late promoter. a dna fragment carrying thymidine kinase coding sequences but lacking the thymidine kinase promoter was sandwiched between a piece of adenoviral dna and simian virus 40 early dna on a plasmid. the aligned fragment was then inserted into the adenoviral genome, replacing internal adenoviral ... | 1985 | 2987934 |
| evidence for the existence of a thermolabile element in adenovirus type 5 that plays a role during an early stage of infection. | in an attempt to learn the molecular mechanism behind the initial steps (before genome expression) of adenovirus infection, the effect of temperature treatment of adenovirus type 5 particles on their infectivity was studied. it was found that adenovirus type 5 could be inactivated by temperature treatment at between 43 and 44 degrees c. both biochemical and biophysical examination of adenovirus particles failed to disclose any significant changes after exposure to temperatures up to 45 degrees c ... | 1985 | 2988228 |
| [virus infections in fishes: etiology, diagnosis and control]. | | 1985 | 2988236 |
| pathologic changes caused by transplacental infection with an adenovirus-like agent in pigs. | five hysterectomy-derived colostrum-deprived pigs housed in individual cages with positive ventilation developed severe skin cyanosis and edema of the subcutaneous tissues in the submandibular, thoracic, and abdominal regions. the 5 pigs were killed 7 to 10 days after birth. eosinophilic intranuclear inclusion bodies were found in endothelial cells of capillary and small blood vessels throughout the body. ultrastructurally, nuclei of these affected endothelial cells contained small crystalline a ... | 1985 | 2988380 |
| c-reactive protein in respiratory virus infections. | | 1985 | 2989473 |
| adenovirus vai rna prevents phosphorylation of the eukaryotic initiation factor 2 alpha subunit subsequent to infection. | the virus-associated vai rna of adenovirus is a small, rna polymerase iii-transcribed species required for efficient translation of mrnas late after infection. deletion mutant dl331 fails to produce this rna and, as a result, grows poorly. three lines of evidence suggest that vai rna facilitates translation by preventing inactivation of the function of eukaryotic initiation factor 2 (eif-2). first, the mutant's translational defect can be relieved by addition of eif-2 or eif-2b (gtp recycling fa ... | 1985 | 2989814 |
| bronchiolitis obliterans and pneumonia induced in young dogs by experimental adenovirus infection. | young beagle dogs were experimentally inoculated with canine adenovirus type 2 and studied by virologic, histologic, immunoperoxidase, and ultrastructural methods from 1 to 26 days after inoculation. virus was recovered from lungs at 2, 3, 5, and 8 days after inoculation. virions and viral antigen were demonstrated by ultrastructural and immunoperoxidase techniques in nonciliated bronchiolar epithelial cells and mucous cells in bronchioles, bronchi, and trachea as well as in bronchial and trache ... | 1985 | 2990219 |
| increase in dna polymerase alpha in nuclei of chicken kidney cells after avian adenovirus infection. | dna polymerase activity which sedimented at about 7s in the presence of 0.5m kc1 increased in cultured kidney cells after infection with a chicken embryo lethal orphan virus, an avian adenovirus. elevated levels of antigenic material to a monoclonal antibody specific for the large subunit of chicken dna polymerase alpha were also detected in nuclei of virus-infected cells by an immunofluorescence method. since the elevations of dna polymerase activity and of nuclear immunofluorescence was not in ... | 1985 | 2991062 |
| adenovirus sequences required for replication in vivo. | we have studied the in vivo replication properties of plasmids carrying deletion mutations within cloned adenovirus terminal sequences. deletion mapping located the adenovirus dna replication origin entirely within the first 67 bp of the adenovirus inverted terminal repeat. this region could be further subdivided into two functional domains: a minimal replication origin and an adjacent auxillary region which boosted the efficiency of replication by more than 100-fold. the minimal origin occupies ... | 1985 | 2991857 |
| adenovirus type 2 encephalitis and concurrent epstein-barr virus infection in an adult man. | a 30-year-old bisexual male prisoner with a history of intravenous drug abuse manifested encephalitis. adenovirus type 2 was isolated from brain tissue obtained by a biopsy and there was a concurrent fourfold rise in the antibody titer. in addition, an initial negative result from a slide test for infectious mononucleosis heterophil antibodies (monospot) test converted to positive and there was an increase in the igg antibody titer to epstein-barr virus (ebv) viral capsid antigen. although he wa ... | 1985 | 2992429 |
| selection of nonfastidious adenovirus species in 293 cells inoculated with stool specimens containing adenovirus 40. | of 35 stool specimens isolated and examined in 293 cells, 15 isolates contained adenovirus species 40 (ad40), and 4 of these 15 isolates also contained a nonfastidious adenovirus species (ad1 in two cases, ad18 or ad31) which was selected over ad40 during serial passage in the 293 cells. the selection of ad1 over ad40 was examined in detail. restriction analysis of intracellular dna and the relative infectivity titers of ad40 and ad1 at each passage level after the inoculation of 293 cells with ... | 1985 | 2993350 |
| simian virus 40 replication in adenovirus-transformed human cells antagonizes gene expression. | simian virus 40 (sv40) replicates efficiently in monkey kidney cells. however, we have now found that sv40-based vectors transfected into most human cells replicate poorly, if at all. in contrast, strong sv40 replication is observed in human embryonic kidney (hek) cells transformed with the adenovirus early region, but not in untransformed hek cells. vector replication in adenovirus-transformed cells is dependent on the presence of the sv40 origin of replication and large-t antigen. however, vig ... | 1985 | 2993921 |
| repression of simian virus 40 early transcription by viral dna replication in human 293 cells. | the small dna tumour virus simian virus 40 (sv40) has served as an excellent model for many studies on the mechanism and control of gene expression in eukaryotic cells. the sv40 early region produces two protein products. one product (large-t antigen) is known both to repress early viral transcription and to stimulate viral replication by binding to specific sites in the origin-promoter region. the early promoter has several similarities to other rna polymerase ii promoters, for example, it poss ... | 1985 | 2993922 |
| fecal rotaviruses, adenoviruses, coronavirus-like particles, and small round viruses in a cohort of rural costa rican children. | excretion patterns of fecal viruses were studied in a cohort of 51 rural costa rican children. the presence of rotavirus, adenovirus, coronavirus-like particles, and small round viruses was investigated by electron microscopy (em) in 2,516 extracts of weekly fecal specimens. rotavirus was in addition studied with elisa. the incidence of diarrhea was 0.7 episodes per child-year. rotavirus was the most common virus (0.53 infection/child-year), followed by adenovirus (0.46 infection/child-year), an ... | 1985 | 2994501 |
| fatal disseminated adenovirus infection featuring liver necrosis and prolonged viremia in an immunosuppressed child with malignant histiocytosis. | truly disseminated adenovirus disease is rare and appears to be limited to patients with severe combined t and b cell deficiency. this report describes an 18-month-old child with malignant histiocytosis treated with chemotherapy who developed a fatal disseminated type 2 adenovirus infection. histologic changes in the tissues of multiple organs as well as the presence of typical crystalline structures in sections of the liver seen with electron microscopy provided evidence of viral replication. a ... | 1985 | 2994509 |
| microbial diagnosis by nucleic acid sandwich hybridization. | sandwich hybridization is a three-component nucleic acid hybridization method suitable for the identification of microbes. in this method, one specific dna fragment on solid support acts as catching reagent, and the second reagent is a labeled probe. the labeling of the support is mediated by a specimen nucleic acid homologous to both reagents. because the specimen is kept in solution, relatively crude specimens not requiring elaborate pretreatments can be tested without background problems. the ... | 1985 | 2994944 |
| spot hybridization for the detection of adenoviruses and enteroviruses. | nucleic acid spot hybridization was used for detection of adenovirus dna directly in clinical specimens and enterovirus rna in infected cells. the test gave results comparable to those obtained with antigen detection assays for adenoviruses. spot hybridization could also be used for detection of enterovirus growth in cell cultures after a single passage from clinical specimens. | 1985 | 2994945 |
| cytoskeletal proteins associated with intracytoplasmic human adenovirus at an early stage of infection. | taking advantage of the sedimentation properties of adenovirus particles, adenovirus-infected baby hamster kidney (bhk21) cells were reversibly fixed with cleavable diimidoester dimethyl 3,3'-dithiobispropionimidate (dtbp) at early times of infection (30 min). cytoskeletal proteins associated with/or in close vicinity to virions were isolated as a complex cross-linked with carrier virus. four major cellular proteins were thus found to co-purify with adenovirus particles. they were characterized ... | 1985 | 2995098 |
| fecal adenoviruses from a longitudinal study of families in metropolitan washington, d.c.: laboratory, clinical, and epidemiologic observations. | during a 29-month period, we studied enteric infection in 70 families from a pediatric practice in suburban washington, d.c. fecal adenoviruses were detected in stools of 18 patients by tissue culture and electron microscopic procedures. from 6 through 11 months of age, the incidence of fecal adenoviruses associated with enteritis was seven per 100, and of confirmed enteric adenoviruses (eads), three per 100 individuals per year. all eads belonged to subgenus g (type 41). all three patients with ... | 1985 | 2995630 |
| [virus-staphylococcal destructive pneumonia in children]. | | 1985 | 2995722 |
| transcription of class iii genes activated by viral immediate early proteins. | the adenovirus eia and pseudorabies virus immediate early (ie) proteins induce transcription from transfected viral and nonviral genes transcribed by rna polymerase ii (class ii genes). these proteins have now been shown also to activate transcription of transfected genes transcribed by rna polymerase iii (class iii genes). as previously observed for class ii genes, this stimulation of class iii gene transcription was much greater for transfected genes than for the major endogenous cellular clas ... | 1985 | 2996135 |
| human epithelial cell carcinogenesis: combined action of dna and rna tumor viruses produces malignant transformation of primary human epidermal keratinocytes. | | 1985 | 2996766 |
| rapid viral diagnosis of acute respiratory infections: comparison of enzyme-linked immunosorbent assay and the immunofluorescence technique for detection of viral antigens in nasopharyngeal secretions. | nasopharyngeal secretions from adults and children were obtained in stockholm, sweden, for routine diagnosis of influenza a virus, influenza b virus, respiratory syncytial (rs) virus, parainfluenza type 3 virus, and adenovirus infections by demonstration of viral antigens directly in the specimens. the cells in nasopharyngeal secretions were pelleted by centrifugation for preparation of cell deposits for diagnosis by the immunofluorescence technique (if) in london, england, and in stockholm, whe ... | 1985 | 2997270 |
| prevalence of antibodies to bovid herpesvirus 1 (ibr-ipv), bovine virus diarrhoea, bovine respiratory syncytial parainfluenza 3, adeno a and adeno b viruses in indigenous and imported moroccan cattle. | a serological survey on prevalence of antibodies to bovid herpesvirus 1 (ibr-ipv) bovine virus diarrhoea, bovine respiratory syncytial, parainfluenza 3, adeno a and b viruses was performed in 524 cattle from different areas and management conditions in morocco. general antibody prevalence was 62.8, 48.5, 70.4, 68.1, 9.0 and 12.4, for the six viruses, respectively. higher prevalence and high antibody titers to most of the investigated viruses were found among cattle from extensive management syst ... | 1985 | 2998260 |
| [diagnostic problems posed by respiratory infections of dogs]. | the following viruses as well as bacteria and mycoplasma have been isolated from dogs with contagious respiratory disease: canine distemper virus; canine adenoviruses (type 1 and 2); parainfluenza type 2 (sv5); reovirus type 1; canine herpesvirus; bordetella bronchiseptica, streptococcus, pasteurella, staphylococcus and mycoplasma. the occurrence of these agents can be in direct relationship with: the evolution of a systemic disease; respiratory disorders being a regular or inconsistant symptom ... | 1985 | 2998696 |
| mouse polyoma virus and adenovirus replication in mouse cells temperature-sensitive in dna synthesis. | mouse adenovirus multiplies, apparently without impediment, in temperature-inactivated ts a1s9, tsc1 and ts2 mouse fibroblasts. thus, the dna of mouse adenovirus can replicate in the absence of functional dna topoisomerase ii, a dna-chain-elongation factor, and a protein required for traverse of the g1/s interface, respectively, encoded in the ts a1s9, tsc1 and ts2 genetic loci. these results are compared with those obtained with polyoma virus. | 1985 | 2999031 |
| dna sequence of the region in the genome of herpes simplex virus type 1 containing the genes for dna polymerase and the major dna binding protein. | in the long unique region of the genome of herpes simplex virus type 1 (hsv-1), the genes for dna polymerase and the major dna binding protein are arranged in a head to head manner, with an origin of dna replication (termed oril) located between them. this paper reports an 8400 base pair dna sequence containing both genes and the origin, obtained mostly by m13/dideoxy analysis of plasmid cloned fragments. amino acid sequences of the two proteins were deduced. homologues of both genes were detect ... | 1985 | 2999714 |
| three-year survey of the epidemiology of rotavirus, enteric adenovirus, and some small spherical viruses including "osaka-agent" associated with infantile diarrhea. | studies were made by electron microscopy (em) on the viruses associated with diarrhea of outpatients at a pediatric clinic in osaka prefecture during the three year period from 1980 through 1982. the viruses detected by em by negative staining with phosphotungstic acid (pta) were classified morphologically into 6 groups: rotavirus, adenovirus and four kinds of small spherical viruses, calicivirus, astrovirus, picornavirus/parvovirus (p/p)-like agent and osaka-agent. osaka-agent seems to be a new ... | 1985 | 3000350 |
| serologic evaluation of vaccinated american river otters. | the oklahoma department of wildlife conservation acquired 20 american river otters (lutra canadensis) between 1984 and 1985 for reintroduction into oklahoma waterways. in 1985, 10 otters were evaluated for serum antibody titers after vaccination with canine distemper virus, canine adenovirus type 2, canine parvovirus (cpv), feline panleukopenia virus (fpv), feline rhinotracheitis virus (frv), and feline calicivirus. prevaccination serum-virus neutralization (svn) antibody to feline rhinotracheit ... | 1985 | 3001000 |
| effects of the adenovirus 2 late promoter on simian virus 40 transcription and replication. | a 100-base-pair fragment of adenovirus 2 (ad2) dna encompassing the major late transcriptional promoter was inserted into the simian virus 40 (sv40) late promoter region at sv40 nucleotide 294 to study the effects of a strong tata box-containing promoter on sv40 late transcription. psvade contains the insert in an orientation such that it would promote transcription towards the origin and early region of sv40, while the insert is in the opposite orientation in psvadl. nuclease s1 analysis with 5 ... | 1986 | 3001338 |
| functional interactions of the simian virus 40 core origin of replication with flanking regulatory sequences. | we constructed a matched set of plasmids to investigate the interactions of essential core sequences of the simian virus 40 replication origin with flanking regulatory sequences. deletions of either t-antigen-binding region i or the 21-base-pair repeated promoter elements reduced replication to 50 to 70% of wild-type levels. the simultaneous deletion of both regions decreased replication to less than 5% of wild-type levels. thus, the double deletion greatly amplified the defects of the single de ... | 1986 | 3001340 |
| [expression vectors for mammalian cells]. | | 1985 | 3001827 |
| [intracellular changes induced by the canine infectious laryngotracheitis virus (cav-2)]. | canine infectious laryngotracheitis virus, an adenovirus designated cav-2, induces numerous changes in the nucleus and cytoplasm of canine kidney cells (mdck). the following features could be observed on ultrathin sections examined in the electron microscope: a shift of the nucleolus towards the nuclear membrane and a compression of the latter by the virus particles; the release of virions into the cytoplasm following the tearing or evagination of the nuclear membrane; the attachment of virus pa ... | 1985 | 3004018 |
| polyradiculoneuritis in children groups during simultaneous circulation of enteroviruses and adenoviruses. | two cases of polyradiculoneuritis in children were noted during january, 1984. each girl was a member of a different group of children: the first group was newly constituted of children from various remote regions of czechoslovakia in a medical institution, the second one included children attending the same nursery school for a long time. in both groups, all or most of the children went through one or two respiratory infections which preceded the development of a paralytic disease. from the fir ... | 1985 | 3004069 |
| generalized venocentric lesions in the virus-associated hemophagocytic syndrome. | clinical and autopsy findings in a 41/2-year-old boy who had been healthy until the onset of virus-associated hemophagocytic syndrome are presented. in addition to findings previously reported in association with the syndrome, widely disseminated perivenular expansile nodules of bland necrosis were seen. these nodules were suggestive of vessel injury, with extravasation of vascular contents and focal destruction of normal elements. the morphologic similarities of these nodules to early lesions o ... | 1986 | 3005155 |
| characteristics of in vitro transformed cells essential for their in vivo survival, selection and metastasizing activity. | two questions were investigated: (1) the dynamics of in vivo selection of tumor cells possessing metastasizing activity (ma) at the time preceding the appearance of visible lung metastases, and (2) the possible connection of in vivo selection of metastatic tumor cells with their susceptibility to h2o2 produced by the cytotoxic oxidative burst of activated neutrophils, monocytes and macrophages. spontaneously in vitro transformed syrian hamster embryo cells (strain sthe), never selected in vivo a ... | 1986 | 3005179 |
| biosynthesis and properties of the adenovirus 2 l1-encoded 52,000- and 55,000-mr proteins. | the adenovirus type 2 l1 region, which is located at 30.7 to 39.2 map units on the viral genome, is transcribed from the major late promoter during both early and late stages of virus replication, and a 52,000-mr (52k) protein-55k protein doublet has been translated in vitro on l1-specific rna. to investigate the biosynthesis and properties of the l1 52k and 55k proteins, we prepared antibody against a synthetic peptide encoded near the predicted n terminus. as determined by immunoprecipitation ... | 1986 | 3005630 |
| rapid diagnosis of viral infections with fluorescent antisera. | viral immunofluorescence tests were performed with commercial antisera on 1046 specimens of respiratory, conjunctival corneal and dermal origin to compare the diagnostic effectiveness of direct examination by immunofluorescence with culture. the fluorescence assays were found to be highly sensitive for respiratory syncytial and measles virus in nasopharyngeal aspirates from children, but less satisfactory for other respiratory viruses. the test detected 38% of the adenovirus positives from conju ... | 1985 | 3005952 |
| [shifts in the antioxidant activity in the membrane structures of virus-infected cells]. | it is assumed that the process of virus infection of cells causes significant changes in the conditions of lipid peroxidation in membrane structures of such cells. experiments with virus-infected hela cells demonstrated noticeable decrease in the capacity of membrane lipids for peroxidation induced by ultraviolet irradiation and iron monoxide ions. similar changes were also observed in membranes of chick embryo liver cells infected with viruses. the established reduced capacity of lipids in memb ... | 1985 | 3006359 |
| life span and 'immortalization' of mammalian cells. | | 1985 | 3007349 |
| inoculation of lambs with ovine adenovirus 5 (mastadenovirus ovi 5) strain rts-42. | twelve 1-week-old colostrum-deprived lambs inoculated with the rts-42 strain of mastadenovirus ovi 5 were killed and necropsied (2 lambs/day) on postinoculation days (pid) 2, 4, 6, 8, 12, and 21. four noninoculated lambs were killed and necropsied (2/day) on pid 6 and 12. virus was isolated from nasal secretions and feces on pid 1 to pid 6, from tracheal fluids and lung tissue of lambs necropsied on pid 2, 4, and 6, and from lung tissue from 1 lamb necropsied on pid 8. virus was not recovered fr ... | 1986 | 3008604 |
| seroepizootiology of types 3 and 7 adenoviruses and bovine viral diarrhea virus infections of beef cattle from birth to first parturition. | twenty-nine female angus cattle were monitored from birth until 24 months of age for infection with types 3 and 7 adenoviruses (av3, av7) and bovine viral diarrhea (bvd) virus by virus isolation and neutralization tests. twenty-seven animals remained in the study for 450 days, whereas 16 animals remained at the conclusion of the tests. viruses were not isolated from test animals. seroepizootiologic examination indicated that there were 28 infections with av3, 33 infections with av7, and 13 infec ... | 1986 | 3008611 |
| simultaneous infections with different enteric and respiratory tract viruses. | infants and young children with rotavirus (rv) or visualized adenovirus in their stools were tested for the simultaneous presence of a respiratory viral pathogen in their upper respiratory tract. overall, at least 10.7% of 484 study subjects had such dual infections, including 8.3% of 385 rv-positive gastroenteritis patients and 24.3% of 37 rv-positive respiratory disease patients. respiratory syncytial virus was present in 34.1% of 41 dual infections with rv and at least 40% of the 12 to 15 dua ... | 1986 | 3009527 |
| studies on fastidious adenoviruses in ontario: a distinct strain associated with gastroenteritis. | from 100 cases of gastroenteritis among children caused by adenovirus infection in ontario, 33 virus isolates were divided into three categories according to their biological behavior in tissue cultures. so far, the results of neutralization tests, structural protein analysis, and dna restriction patterns showed that the virus of category 1 was similar to adenovirus type 40. however, the adenovirus of category 2 was a distinct adenovirus which shared some similarities with adenovirus type 5. vir ... | 1986 | 3009537 |
| acute lower respiratory tract infections in nonhospitalized children. | | 1986 | 3009769 |
| va rnas from avian and human adenoviruses: dramatic differences in length, sequence, and gene location. | human adenoviruses encode low-molecular-weight rnas, so-called va rnas, which are transcribed by rna polymerase iii. these rnas are required for an efficient translation of viral mrnas late after infection. the genes for the va rnas in the genome of celo virus were mapped and characterized. the results showed a number of surprising differences between celo virus and human adenovirus type 2 (ad2). thus, the celo virus genome encoded only one va rna species, in contrast to human ad2, which encoded ... | 1986 | 3009871 |
| diffuse intestinal ulceration after marrow transplantation: a clinicopathologic study of 13 patients. | the cases of 13 allogeneic marrow transplant recipients who had undergone laparotomy for manifestations of severe enteritis were reviewed to determine the causes of the severe intestinal disease and to assess the relation between clinical, histologic, and microbiologic findings. laparotomies were performed a median of 63 days (range, 11 to 273 days) after transplantation for suspected peritonitis, intestinal obstruction, or bleeding. intestinal tissue was available from small bowel resections in ... | 1986 | 3011641 |
| homology between dna polymerases of poxviruses, herpesviruses, and adenoviruses: nucleotide sequence of the vaccinia virus dna polymerase gene. | a 5400-base-pair segment of the vaccinia virus genome was sequenced and an open reading frame of 938 codons was found precisely where the dna polymerase had been mapped by transfer of a phosphonoacetate-resistance marker. a single nucleotide substitution changing glycine at position 347 to aspartic acid accounts for the drug resistance of the mutant vaccinia virus. the 5' end of the dna polymerase mrna was located 80 base pairs before the methionine codon initiating the open reading frame. corre ... | 1986 | 3012524 |
| [viral respiratory infections in children: new diagnostic methods for early detection. initial results of a pilot project in switzerland]. | the new methods of rapid viral diagnosis make it possible to specify a number of the most prevalent agents of respiratory tract infections within 24 hours. the techniques are based on the immunological detection of antigens of respiratory syncytial (rsv), adeno, parainfluenza type 1, 2 and 3, as well as of influenza a and b viruses in nasopharyngeal secretions. during a one-year period we have used these methods to evaluate diagnostically 1541 outpatients presenting with upper and lower respirat ... | 1986 | 3012772 |
| nitrogen metabolism of calves inoculated with bovine adenovirus-3 or with infectious bovine rhinotracheitis virus. | beef calves were inoculated with bovine adenovirus-3 or infectious bovine rhinotracheitis virus. after inoculation, plasma fibrinogen increased, serum phosphorus decreased, and nitrogen and phosphorus digestibility decreased compared with preinoculation values. urinary n excretion increased when calves developed rectal temperatures greater than 39.7 c. results indicated that clinical infection of calves with infectious bovine rhinotracheitis virus increases urinary n excretion and reduces n and ... | 1986 | 3013053 |
| acceleration of scrapie disease in mice by an adenovirus. | coinfected mice were examined for a possible interaction between the scrapie agent and an adenovirus. a low titer (10(2) tcd50) of mouse adenovirus (madv) caused a significant acceleration of clinical signs of scrapie in mice infected 128 days previously with scrapie. in this experiment, the coinfected mice died 19 days earlier than mice infected with scrapie alone. when a higher titer of madv (10(4)-10(5) pfu) was used, a more drastic acceleration of scrapie disease was seen in mice infected 85 ... | 1986 | 3013796 |
| rapid diagnosis of respiratory adenovirus infections in young adult men. | rapid viral diagnosis was attempted in 106 military conscripts with pneumonia and in 101 military conscripts with other types of respiratory infections. nasopharyngeal suction specimens (nps) were assayed for viral antigens by immunofluorescence and enzyme immunoassay (eia). sputum specimens from 97 pneumonia patients were assayed for viral antigens by eia. also, 71 nps and 13 sputum specimens were examined for the presence of adenovirus dna by a sandwich hybridization (hyb) method. the referenc ... | 1986 | 3013925 |
| acute hemorrhagic cystitis caused by adenovirus type 11 after renal transplantation. | a case of acute hemorrhagic cystitis caused by adenovirus type 11, which occurred in an allograft recipient 6 months after a living-related renal transplantation, is described. the patient lacked a neutralizing antibody to adenovirus type 11 before transplantation. adenovirus type 11 was isolated from his urine and he developed a neutralizing and complement-fixing antibody to this virus. although adenovirus type 11 isolates had been obtained from 2 of 18 renal allograft recipients, only 1 patien ... | 1986 | 3014702 |
| involvement of spleen components of mature fowl in the primary and secondary humoral antibody response following experimental eds'76 virus infection. | cellular changes in spleens of mature fowl in relation to both the primary and secondary humoral antibody response following experimental eds'76 virus infection were studied. the influence of splenectomy on humoral antibody response was also examined. experimental fowl had been naturally infected with fowl adenovirus (fav) but did not possess precipitins to these viruses at the time of eds'76 virus infection. since eds'76 infection provokes a recall of the group antibody to fav, this infection s ... | 1986 | 3014709 |
| enzyme linked immunosorbent assay used to monitor serum antibodies to bovine respiratory disease viruses. | an enzyme linked immunosorbent assay (elisa) was applied to the detection of serum antibodies against infectious bovine rhinotracheitis (ibr), parainfluenza-3 (pi3), adenovirus type 3 (adeno 3) and bovine respiratory syncytial (brs) viruses. paired serum samples from calves vaccinated with live attenuated virus vaccines were tested. the elisa compared favorably with the virus neutralization test for detecting serologic responses to ibr, brs, and adeno 3 viruses or with the hemagglutination inhib ... | 1986 | 3014711 |
| in utero adenoviral infection of sheep. | the occurrence of natural in utero adenovirus infection in sheep was examined. isolation of three adenovirus strains from the kidneys of 174 sheep foetuses is reported; all three isolates belonged to type 2 bovine adenovirus (bav 2) subtype b. five of 25 blood samples from sheep foetuses contained virus-neutralizing antibodies (1:4-1:24) against bav 2. these data prove that transplacental transfer of adenovirus infection can occur naturally in sheep. | 1986 | 3014715 |
| [effect of antioxidants on the hela cell infection process in culture]. | a certain role in the outcome of virus infection of cells was assumed to belong to cell membrane lipids peroxidation. the influence of known inhibitors of lipid peroxidation, beta-naphthol and ionol, on hela cells infection with human adenovirus type 2 was studied. these antioxidants in certain concentrations were found to be capable of effectively inhibiting virus infection of hela cell cultures. at the same time, beyond these concentration levels these antioxidants did not inhibit but stimulat ... | 1986 | 3014753 |
| early interactions between animal viruses and the host cell: relevance to viral vaccines. | viral recognition of specific receptors in the host cell plasma membrane is the first step in virus infection. attachment is followed by a redistribution or capping of virus particles on the cell surface which may play a role in the uptake process. certain viruses penetrate the plasma membrane directly but many, both enveloped and non-enveloped viruses, are endocytosed at coated pits and subsequently pass into endosomes. the low ph environment of the endosome facilitates passage of the viral gen ... | 1986 | 3014773 |
| an unusual case of inclusion body hepatitis in a cockerel. | this report describes inclusion body hepatitis in a 12-week-old leghorn-type cockerel. the cockerel had come from a specific-pathogen-free flock and was reared in a positive-pressure isolator until 6 weeks old. grossly, the cockerel was jaundiced, and the liver was swollen and had small white foci throughout. histologically, there were foci of hepatic necrosis and basophilic inclusion bodies in the nuclei of numerous hepatocytes. a fowl adenovirus, isolated from the liver, was found to belong to ... | 1986 | 3015107 |
| pathology of avian adenovirus serotypes in the presence of escherichia coli in infectious-bursal-disease-virus-infected specific-pathogen-free chickens. | ten strains of adenovirus representing 10 serotypes were administered intratracheally to 3-week-old specific-pathogen-free chickens, which also received 2.5 x 10(5) colony-forming units of a pathogenic escherichia coli intranasally. those birds had been inoculated by eye drop at 1 day of age with a virulent infectious bursal disease virus (ibdv). controls consisted of groups of chickens inoculated with: (a) ibdv and e. coli, (b) ibdv only, (c) e. coli only, and (d) no virus or e. coli. gross pul ... | 1986 | 3015109 |
| human viruses in sediments, sludges, and soils. | recent studies have provided a greater understanding of the movement of viruses in the environment by their attachment to solids. these studies have focused on solids-associated viruses present in wastewater discharged into the ocean and on viruses in sludge and wastewater that may be retained in soil following their land disposal. such ocean or land disposal of wastewater and sludge may result in a discharge of one or more of 120 human enteric virus pathogens including those causing poliomyelit ... | 1986 | 3015442 |
| eukaryotic dna replication. | | 1986 | 3017196 |
| the set of growth factors stimulatory for a transformed rat cell of line nrk-49f depends on the identity of the transforming virus. | re-cloned rat fibroblast line nrk-49f was transformed by human adenovirus type 5 which had been grown in human cells of the kb carcinoma line. cell subclones were isolated from seven independent transformation events, and seven untransformed cell subclones were isolated in parallel from control cultures which had been inoculated with lysate of uninfected kb cells. the adenovirus-transformed cells differed from the control untransformed fibroblasts by being typically small and cuboidal, showing m ... | 1986 | 3018909 |
| interaction of immunosuppressive agents and human adenovirus infection in mice. | it was examined whether sensitivity of mice to human adenovirus infections is affected by immunosuppressive agents. mice treated with the lymphotropic cytostatic dianhydrodulcitol (dad) or antilymphocytic serum (als) did not become susceptible to human adenovirus type 12 infection, as the virus did not replicate--not even in its component forms--in the animals. on the other hand, the effect of both dad and als on the lymphoid organs of mice was intensified by human adenovirus infection. in tissu ... | 1986 | 3019068 |
| regulation of protein synthesis in virus-infected animal cells. | | 1986 | 3019107 |
| viral conjunctivitis with special reference to adenovirus type 37 and enterovirus 70 infection. | the clinical and etiological findings in 727 patients with viral conjunctivitis treated from january 1982 through december 1984 at aoki eye clinic in sapporo, japan, were presented. the age of the patients ranged from 11 days to 88 years, and the monthly incidences of the disease from 18 to 131 cases, with a clustering in the summer season. the etiological diagnosis was established in 399 (54.9%) of the 727 patients: adenovirus (ad) 3 in 45 cases; ad4 in 98 cases; ad8 in 57 cases; ad19 in 23 cas ... | 1986 | 3020290 |
| codon insertion mutants of the adenovirus terminal protein. | a series of codon insertion mutants was isolated following restriction site-directed linker insertion mutagenesis of the open reading frame for the type 5 adenovirus terminal protein precursor. the conditionally lethal mutant h5sub100 bears an insertion mutation upstream of the first aug in the reading frame, fails to replicate its dna under nonpermissive conditions, and was assigned to the terminal protein complementation group. these data establish that terminal protein is an essential polypep ... | 1986 | 3020558 |
| characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes. | three monoclonal antibodies were characterized by examining their reactivity to human cytomegalovirus (hcmv) glycoproteins under reducing and nonreducing conditions and their reactivity to glycoproteins and disulfide-linked glycoprotein complexes isolated by ion-exchange high-performance liquid chromatography. one monoclonal antibody, 9e10, reacted with glycoprotein complexes which had molecular weights of 93,000 and 450,000 and eluted from the ion-exchange column at 0.3 and 0.9 m nacl, respecti ... | 1986 | 3021969 |
| genomic organization of the simian virus 40-adenovirus 7 hybrid virus, para(ct), that encodes a nuclear transport defective simian virus 40 t antigen. | the genomic organization of the simian virus 40 (sv40)-adenovirus (ad)7 hybrid virus, para(ct), was examined. a deletion of approximately 5529 bp of ad7 dna extends from 78.8 map units to 94.0 map units and is replaced by an sv40 dna insert of 3809 bp. the left-hand end of the insertion begins at sv40 nucleotide 5168, 5 bp upstream of the atg initiation codon for t-ag synthesis. the sequence extends counterclockwise through the t-ag encoding sequences and into sv40 late region dna. most of the l ... | 1986 | 3022475 |
| transcription activation by viral and cellular oncogenes. | | 1986 | 3022565 |
| comparative biological characterization of mouse adenovirus strains fl and k 87 and seroprevalence in laboratory rodents. | the growth, stability and seroprevalence in laboratory rodents of the two known strains of mouse adenovirus were compared. the fl strain of mouse adenovirus grew in both l 929 murine fibroblasts and in cmt-93 murine rectal carcinoma cells, whereas the k 87 strain grew only in cmt-93 cells. the bulk of the fl progeny virus was released from the host cells. k 87 virus was largely cell-associated. both virus strains were stable at 37 degrees c in liquid medium. the k 87 strain was completely inacti ... | 1986 | 3022678 |
| partition of e1a proteins between soluble and structural fractions of adenovirus-infected and -transformed cells. | the partition of e1a proteins between soluble and structural framework fractions of human cells infected or transformed by subgroup c adenoviruses was investigated by using gentle cell fractionation conditions. a polyclonal antibody raised against a trpe-e1a fusion protein (k.r. spindler, d.s.e. rosser, and a. j. berk, j. virol. 132-141, 1984) synthesized in escherichia coli was used to measure the steady-state levels of e1a proteins recovered in the various fractions by immunoblotting. the rela ... | 1986 | 3023654 |
| cellular mrna translation is blocked at both initiation and elongation after infection by influenza virus or adenovirus. | during influenza virus infection, protein synthesis is maintained at high levels and a dramatic switch from cellular to viral protein synthesis occurs despite the presence of high levels of functional cellular mrnas in the cytoplasm of infected cells (m. g. katze and r. m. krug, mol. cell. biol. 4:2198-2206, 1984). to determine the step at which the block in cellular mrna translation occurs, we compared the polysome association of several representative cellular mrnas (actin, glyceraldehyde-3-ph ... | 1986 | 3023655 |
| preferential clustering of viral dna sequences at or near the site of chromosomal rearrangement in fowl adenovirus type 1 dna-transformed cell lines. | all six transformants obtained by inoculating fowl adenovirus type 1 (celo virus) dna or its fragments into a rat cell line of normal karyotype had more than 50 copy-equivalents of viral dna sequences. each of the transformants had almost all if not all of these viral dna sequences clustered on a marker chromosome(s). although the marker chromosome(s) differed from one cell line to another, viral dna sequences preferentially clustered in or near the achromatic (or light-stained) region of the g- ... | 1987 | 3023693 |
| downstream sequences affect transcription initiation from the adenovirus major late promoter. | we analyzed a set of adenovirus-simian virus 40 (sv40) hybrids in which the sv40 t antigen coding sequences are inserted downstream from the adenovirus major late promoter within the first, second, and third segments of the tripartite leader. in infected cells, these viruses give rise to a matched set of hybrid sv40 mrnas that differ only in the number of tripartite leader segments attached to the complete sv40 t antigen coding region. we found that the number of tripartite leader segments prese ... | 1986 | 3023944 |
| construction of a helper-free recombinant adenovirus that expresses polyomavirus large t antigen. | adenovirus-polyomavirus recombinant viruses were constructed in vitro by inserting a hybrid transcription unit composed of the adenovirus type 2 major late promoter and the early coding region of polyomavirus into the adenovirus type 5 vector ad5 delta e1/dl309. the vector lacks the e1a and e1b transcription units and contains a unique restriction endonuclease cleavage site in their place. the polyomavirus genomic insert contained a small deletion which precluded the synthesis of functional smal ... | 1986 | 3023952 |
| [transforming genes of dna tumor viruses sv 40, polyoma virus, and adenoviruses]. | | 1986 | 3024234 |
| adenovirus type 3 infection with systemic manifestation in apparently normal children. | between july 1983 and february 1984, eight children with adenovirus type 3 infection, proven by virus isolation from sputum, stool or nasopharyngeal swabs and a fourfold increase in complement fixation antibody titers against the virus, were treated in our department. all eight patients had fever lasting at least 7 days, hepatomegaly, diffuse pulmonary infiltrates and abnormal liver function tests. seven of the patients exhibited dyspnea and pulmonary wheezing. six of the patients developed chan ... | 1986 | 3025130 |
| [acute respiratory infections among infants and children in japan. the first report: virus isolation employing a new microplate method]. | | 1986 | 3025311 |
| [viral etiology of diarrheal diseases in madagascan children]. | our study included a total of 318 diarrheic stools and 52 normal stools collected from out-patients with acute diarrhea at welfare center and children admitted at antananarivo city children's hospital, or control free of infectious disease during 8 months period. enzyme linked immunosorbant assay and tissue cultures revealed the presence of 152 viral particles (47%) from children with diarrhea and 29 viral particles (55%) from control children. positive cases were distributed according age, sex, ... | 1986 | 3026265 |
| [a comparison of two antigen strains of each of mouse hepatitis virus and mouse adenovirus for detection of complement fixation antibody in mouse and rat sera]. | detection rates of complement fixation antibodies in mice and rats were compared between two antigen strains of each of mouse hepatitis virus (mhv) and mouse adenovirus (mav). among 66 and 47 naturally infected mhv-positive sera of mice (18 facilities) and rats (16 facilities) respectively, 17 mouse and 21 rat sera reacted with both nu-67 and mhv-2 strains, but 49 mouse and 25 rat sera were positive to nu-67 strain alone. only one rat serum reacted with mhv-2 strain alone. in comparison with k87 ... | 1986 | 3026815 |
| a serological survey on 15 murine pathogens in mice and rats. | a serological survey for 15 murine pathogens was performed on 269 mouse sera collected from 21 conventional and 12 barrier colonies, and on 376 rat sera collected from 21 conventional and 23 barrier colonies. animals having an antibody against at least one of the antigens were contained in 81.0% of conventional and 16.7% of barrier mouse colonies and also in 81.0% of conventional and 43.5% of barrier rat colonies. main contaminants were mouse hepatitis virus and sendai virus in mice, and sendai ... | 1986 | 3026818 |