| factors affecting morphine uptake into kidney slices. | | 1976 | 7262 |
| impairment of hepatic microsomal drug metabolism in the rat during daily disulfiram administration. | | 1976 | 7264 |
| studies on the rate of incorporation of tryptophan into nicotinamide-adenine dinucleotides in rats chronically exposed to carbon disulphide. | | 1976 | 7267 |
| the role of cytochrome p-450 in the toxicity of fluroxene (2,2,2-trifluoroethyl vinyl ether) anaesthesia in vivo. | | 1976 | 7270 |
| the interaction of hepatic microsomal cytochrome p-450 with fluroxene (2,2,2-trifluoroethyl vinyl ether) in vitro. | | 1976 | 7271 |
| in vitro inhibition of oxidative n-demethylation with carbon disulfide. | earlier findings have shown that in experimental animals (rat) and in man inhaled carbon disulphide (cs2) reversibly inhibits the non-specific oxidative drug metabolism caused by hepatic microsomal enzymes. very little is known concerning the underlying mechanism. the present investigations were undertaken to throw light on this question. after addition of an nadph-regenerating system to liver microsomes isolated from adult female wistar rats, the oxidative n-demethylation of aminopyrine was mea ... | 1976 | 7280 |
| effects of substrate and inhibitor binding on thermal and proteolytic inactivation of rat liver transhydrogenase. | the thermostability and proteolytic inactivation of rat liver submitochondrial particle transhydrogenase was studied in the presence of pyridine dinucleotide substrates and a variety of divalent metal and nucleotide inhibitors. relative to the unliganded enzyme, the nadph-enzyme complex was more thermostable and showed a twofold greater rate of tryptic inactivation, while the nadp+-enzyme complex was more thermolabile and only slightly more susceptible to tryptic inactivation. neither nad+ nor n ... | 1976 | 7289 |
| purification and properties of atpase inhibitor from rat liver mitochondria. | (1) the atpase inhibitior protein has been isolated from rat liver mitochondria in purified form. the molecular weight determined by sodium dodecyl sulfate gel electrophoresis is approximately 9500, and the isoelectric point is 8.9. (2) the protein inhibits both the soluble atpase and the particle-bound atpase from rat liver mitochondria. it also inhibits atpase activities of soluble f1, and inhibitor-depleted submitochondrial particles derived from bovine heart mitochondria. (3) on particle-bou ... | 1976 | 7295 |
| ammonia production by the small intestine of the rat. | 1. slices of duodenum and jejunum produce ammonia from glutamine in vitro. 2. ammoniagenesis does not increase in response to acidosis or potassium deficiency, two conditions known to cause enhanced ammoniagenesis in the kidney. 3. gut contains glutaminase 1 as well as gamma-glutamyl transpeptidase. 4. these enzymes do not show any increase during starvation. | 1976 | 7312 |
| properties of highly purified lysyl oxidase from embryonic chick cartilage. | lysyl oxidase the enzyme which oxidately deaminates lysine residues in collagen and elastin, was purified from embryonic chick cartialge by employing an affinity column of lathyritic rat skin collagen coupled to sepharose, followed by separation on deae-cellulose. an enzyme preparation was obtained which was pure as shown by polyacrylamide gel electrophoresis. the specific activity was 1800-fold higher than that of the original extract. the pure enzyme utilized both collagen and elastin substrat ... | 1976 | 7318 |
| effect of blocking the neuronal and extraneuronal uptake of bioamines on the adrenosensitizing action of tricyclic antidepressants. | tests conducted on isolated and denervated preparations of the rat seminal duct brought evidence that tricyclic antidepressants (melipromine, noverile and azaphen) when employed in low concentrations (1-10(-9) g/ml) produced an adrenosensitizing effect. denervation with the subsequent block by desoxycorticosterone (1-10(-5) g/ml) of exteraneuronal amine uptake did not alter the position, shape and inclination of the "concentration-effect" noradrenaline curves received in the presence of noverile ... | 1976 | 7328 |
| modulatory role of glucocorticoids on ngf-mediated enzyme induction in organ cultures of sympathetic ganglia. | | 1976 | 7346 |
| mechanisms of the absorption of water-soluble dyes from the rat small intestine. | | 1976 | 7361 |
| assessment of the functional activity of human lymphocytes in malignant disease by the local graft-versus-host reaction in rats and the t rosette-forming cell test. | the local graft-versus-host reaction (gvhr) and the spontaneous rosette-forming cell (rfc) test were used to test the functional activity of lymphocytes in blood obtained from twenty normal donors and thirty patients with malignant tumours. the lymphocytes of all twenty control donors gave a positive gvhr and had normal rfc values. in twenty-two of the thirty patients with malignancies the gvhr was negative; of these, only five had low rfc values. in the remaining eight patients the gvhr was pos ... | 1976 | 7371 |
| substance p and excitatory transmitter of primary sensory neurons. | | 1976 | 7378 |
| synapse formation and neurotrophic effects on muscle cell lines. | | 1976 | 7383 |
| physiological and morphological studies on developing sympathetic neurons in dissociated cell culture. | | 1976 | 7384 |
| synaptic chemistry of identified cells in the vertebrate retina. | | 1976 | 7386 |
| prolactin-releasing and release-inhibiting factor activities in the bovine, rat, and human pineal gland: in vitro and in vivo studies. | the effects of crude extracts of bovine, rat, and human pineal glands on prolactin (prl) release were studied using an in vitro system. in addition, the effects of a known pineal constituent, arginine vasotocin (avt), and crude bovine pineal extract (bpe) on prl secretion were studied in vivo. normal male rat hemipituitaries (hp), incubated with bpe (13 mg tissue/hp)released 200%, 150%, and 285% more prl into the medium than did their corresponding untreated control halves incubated in either me ... | 1976 | 7446 |
| adenylyl cyclase activities in ovarian tissues. i. homogenization and conditions of assay in graafian follicles and corpora lutea of rabbits, rats, and pigs: regulation by atp, and some comparative properties. | responsiveness of ovarian adenylyl cyclases to luteinizing hormone (lh), found to be 5 to 10-fold in cell-free preparations under optimal conditions, required gentle homogenizations and storage in sucrose-containing media. assay conditions required the use of an atp-regenerating system consisting of creatine kinase, creatine phosphate, and myokinase for the preservation of atp levels. lh-stimulated adenylyl cyclase (ac) in rabbit cl showed the following properties: 1) the ph optimum of basal act ... | 1976 | 7447 |
| further observations on the properties of serum and tissue guanase from man and some animal species. optimum ph and activation energy in the presence of 8-azaguanine. | the activation energy and the optimum ph of guanine deaminase in man, the rat, guinea pig and mouse were studied using 8-azaguanine as a substrate. the serum guanase in man and in all the animal species studied differs in activation energy from the guanase of the liver. in man, moreover, the serum guanase is also different from the brain and kidney enzyme. in the rat and guinea pig the brain enzyme has thermic activation energy different from the liver and kidney enzyme. the guanase of the serum ... | 1976 | 7449 |
| interconversion between 17 beta-hydroxy-5alpha-androstan-3-one (5alpha-dihydrotestosterone) and 5alpha-androstane-3alpha, 17 beta-diol in rat kidney: heterogeneity of 3alpha-hydroxysteroid oxidoreductases. | 3alpha-hydroxysteroid oxidoreductases catalyzing the interconversion between 17 beta-hydroxy-5alpha-androstan-3-one (5alpha-dihydrotestosterone) and 5alpha-androstane-3alpha, 17 beta-diol (3alpha-androstanediol) have been studied in rat kidney. three enzymes can be distinguished: a soluble nadph-dependent oxidoreductase, a microsomal nadph-dependent enzyme and a microsomal nadh-linked enzyme. traces of the microsomal enzymes are consistently observed in the 108 000 x g supernatant. studies on cr ... | 1976 | 7455 |
| a review. innate resistance in malaria. | | 1976 | 7463 |
| muscle metabolism investigated at rest and during exercise and/or pharmacological treatment by vasodilators. | the ruderman's preparation was utilized to investigate in situ some aspects of the muscular metabolism both at rest and during submaximal exercise upon bilateral sciatic nerve stimulation, and/or during perfusion with some vasodilators: papaverine, caffeine, nicergoline, bamethan. o2 and glucose uptake, the production of lactate and the glycogen level of the muscle were studied. the availability of acetoacetate modifies the muscular metabolism; other fuels (e.g. the ketone body itself) are proba ... | 1976 | 7472 |
| studies on the molecular species of dna polymerase extracted from rat ascites hepatoma cells. | dna polymerase ec 2.7.7.7 activities present in hypotonic extract from rat ascites hepatoma ah130 cells were eluted in three separable peaks on deae-cellulose column chromatography. peak i activity had an alkaline ph optimum, and was relatively resistant to sh-blocking reagents and salt concentration. these properties of deae peak i are typical of low molecular weight dna polymerase. deae peak ii and peak iii activities possessed properties corresponding to high molecular weight (6-8 s) polymera ... | 1976 | 7555 |
| an oxygraphic method for the determination of cholesterol and measurement of the slow oxygen-consuming reactions of hepatic microsomes. | oxygen-consuming reactions of cholesterol oxidase ec 1.1.3.6 and microsomes were measured with a galvanic oxygen electrode which was attached to an offset amplifier for sensitive measurement of the reaction processes. the sensitivity of this oxygraphic method for detection of oxygen consumption was ten times greater than that of the usual method. the minimum rate of slow oxygen-consuming reactions which could be estimated was about 5 nmoles of oxygen per min, and the minimum amount of oxygen con ... | 1976 | 7559 |
| studies on the subcellular localization and properties of bis(monoacylglyceryl)phosphate biosynthesis in rat liver. | phosphatidylglycerol conversion to bis(monoacylglyceryl)phosphate by rat liver homogenate was studied and maximum rates of synthesis were observed at ph 4.4. the distribution of bis(monoacylglyceryl)p synthetase in rat liver subcellular fractions was determined, and evidence is presented establishing the lysosomes as the site of bis(monoacylglyceryl)p synthesis. in addition to phosphatidylglycerol, 1-acyl- and 2-acyllysophosphatidylglycerol also served as precursors for bis(monoacylglyceryl)p wi ... | 1976 | 7562 |
| properties of the guanylate cyclase-guanosine 3':5'-monophosphate system of rat renal cortex. activation of guanylate cyclase and calcium-independent modulation of tissue guanosine 3':5'-monophosphate by sodium azide. | the effects of sodium azide on guanylate cyclase activity of homogenates of rat renal cortex and on the guanosine 3':5'-monophosphate (cgmp) content of cortical slices were examined and compared to those of carbamylcholine and naf. in complete krebs-ringer bicarbonate buffer containing 10 mm theophylline, tissue cgmp content was increased 5- to 6-fold by 0.05 mm carbamylcholine or 10 mm nan3, and 3-fold by 10 mm naf. increases in cgmp were maximal in response to these concentrations of the agoni ... | 1976 | 7563 |
| milk lipoprotein lipases: a review. | lipoprotein lipase activity has been found in the milks from severals species where it is assumed to result from leakage from the mammary gland into milk. the function of the enzyme in the gland is apparently to assist in the transfer of blood lipoprotein triacylglycerol fatty acids into milk triacylglycerols. bovine skim milk is one of the richest sources of lipoprotein lipase and this enzyme has been purified extensively (7000 fold) by affinity chromatography. the lipase has a molecular weight ... | 1976 | 7579 |
| the effect of pertussis and beta adrenergic-blocking agents on mast cells. | pertussis vaccine injected ip in doses known to cause hypersensitization resulted in a marked decrease in the number of mast cells from the peritoneal washings of rats and mice. a significant reduction was obtained as early as one day after pertussis injection of ten billion cells in rats and was marked after 5 to 7 days. a maximum reduction in the number of mast cells was obtained by a dose of 20 billion cells. there was no detectable histamine biological activity in the supernatant from perito ... | 1976 | 7584 |
| differences between the anorexic actions of amphetamine and fenfluramine--possible effects on hunger and satiety. | the inhition of feeding in rats brought about by amphetamine and fenfluramine was continuously monitored for periods of up to 24 h using a pellet detecting eatometer. for rats tested under conditions of food deprivation the two drugs gave rise to distinctive anorexic profiles: amphetamine delayed the onset of eating whereas fenfluramine allowed eating to commence normally but brought about an early termination of the initial bout of feeding. when the drugs were administrated to rats with free ac ... | 1976 | 7642 |
| alpha-methyl derivatives of biogenic amines as inhibitors of monoamin oxidase. | the values of km app and vmax for three natural substrates of monoamine oxidase have been determined at various stages in the isolation of the enzyme from rat liver tissue. the results are consistent with the presence in the enzyme preparation of at least two distinct molecular forms of the enzyme. using the alpha-methyl derivatives of the natural substrates as inhibitors of the enzyme, the substrate dependence of ki further substantiates this view. in addition, the kinetics of the inhibition su ... | 1976 | 7643 |
| comparative study of the effects of mianserin, a tetracyclic antidepressant, and of imipramine on uptake and release of neurotransmitters in synaptosomes. | the effects of mianserin, a tetracyclic antidepressant, on uptake and release of 3hnoradrenaline (3h-na), 3hdopamine (3h-da), 3h-5-hydroxytryptamine(3h-5-ht) and 3hgamma-amino-butyric acid (3h-gaba) in synaptosomes from different areas of the rat brain were investigated in a comparative study with the tricyclic antidepressant imipramine. mianserin and imipramine were inhibitors of 3h-na uptake in the hypothalamus, but could not increase 3h-na release from noradrenergic nerve endings. this behavi ... | 1976 | 7644 |
| the absorption of warfarin from the rat small intestine in situ. | the in situ absorption from the rat small intestine of the weakly acidic drug, warfarin (pka 5-05), at 200 mug ml-1 in the instilled fluid with initial ph levels of 3, 5, 7 or 8 has been examined. these initial ph's in the buffer changed rapidly towards neutrality. the buffers at ph's 3 and 5 probably caused different amounts of warfarin precipitation, which resulted in different rates of warfarin disappearance from the instilled fluid which paralleled the initial rates of accumulation of warfar ... | 1976 | 7646 |
| the influence of dihydroxyanthracene derivatives on water and electrolyte movement in rat colon. | the purgative activities of 18 different dihydroxyanthracene derivatives, including free anthraquinones and anthrones, were investigated by determining their influence on the water, sodium and potassium absorption in the gastrointestinal tract by direct injection of the solutions in tyrode to the rat colon in situ. the extent of the solubility of the compounds has also been assessed. the 1,8-dihydroxyanthracene structure seemed to be the best for hydragogue effect. rhein-anthraquinone and -anthr ... | 1976 | 7647 |
| a dual action of 5-hydroxytryptamine on the ovarian suspensory ligament of the rat. | 5-hydroxytryptamine has a dual effect on the spontaneously contracting rat ovarian ligament, in vitro, a contraction which is antagonized by the prior administration of methysergide and a relaxation of the ligament observed in the methysergide-treated preparation. the relaxatory effect was not antagonized by propranolol or tetrodotoxin but treatment of the ligament with indomethacin abolished this response. prostaglandins of the e series produced an inhibition, and pgf2alpha a contraction of the ... | 1976 | 7648 |
| response of single raphé neurons to (+)-lsd: correlation with (+)-lsd binding in brain. | | 1976 | 7653 |
| an improved method of estimation of small amounts of antidiuretic activity. | | 1976 | 7655 |
| some observations on the attempted separation of isoenzymes of monoamine oxidase. | | 1976 | 7656 |
| serotoninergic modulation of the dopamine response from the nucleus accumbens. | | 1976 | 7657 |
| dopamine in substantia nigra and cortex after gamma-butyrolactone treatment. | | 1976 | 7658 |
| the effect of sunflower seed oil on freund's adjuvant-induced arthritis in the rat. | | 1976 | 7660 |
| stereoselective and calcium-dependent contractile effects of narcotic antagonist analgesics in the vascular smooth muscle of the rat. | in patients, pentazocine administered i.v. can have an unusual action for a strong analgesic-an elevation of blood pressure. the objective of this study in rats was to better quantify and explain the molecular mechanism for the vascular action of l-pentazocine and compare it with other analgesics and narcotic antagonists. in anesthetized rats, l-pentazocine (0.3-3 mg/kg i.v.) elevated blood pressure and this effect was potentiated in pithed rats. the contraction appeared to be nonadrenergic as i ... | 1976 | 7664 |
| potassium release from the rat submaxillary gland in vitro. i. induction by catecholamines. | slices of submaxillary gland were incubated in vitro in an enriched krebs-ringer-bicarbonate medium gassed with 95% o2-5% co2 at 37 degrees c and the release of k+ into the medium was monitored after stimulation with alpha and beta adrenergic secretagogues under a variety of experimental conditions. k+ was released by the slice system after addition of norepinephrine, epinephrine or phenylephrine, but not after addition of isoproterenol. the extent of k+ release after norepinephrine depends on t ... | 1976 | 7665 |
| comparison of uptake and binding of disodium cromoglycate and phenol red in rat lung. | in rat lung slices 3h-disodium cromoglycate (3h-dscg) (0.001 mm) was taken up rapidly and 3h-dscg tissue spaces, which equilibrated by 30 minutes, remained constant over a 4-hour incubation period. in contrast, 35s-phenol red (0.001 mm) accumulated in lung slices to a much greater extent than did dscg, and the measured tissue spaces continued to increase over a 3-hour incubation period. in the presence of either phenol red (1 mm) or the metabolic inhibitors, iodoacetic acid (10(-4) m) and dinitr ... | 1976 | 7666 |
| the pharmacology of flazalone: a new class of anti-inflammatory agent. | the anti-inflammatory activity of flazalone, a unique chemical drug, is described. in acute irritant anti-inflammatory tests, flazalone exhibited a wide spectrum of activity. the compound was active in affecting the course of paw swelling in adjuvant arthritis when given daily either at the outset of the polyarthritis or after induction. the most unusual aspect of this compound is its ability to inhibit graft rejection in goldfish and rabbits. the pattern of anti-inflammatory activity does not a ... | 1976 | 7668 |
| the association between acidification and electrogenic events in the rat proximal jejunum. | 1. simultaneous measurement of hydrogen ion production, transmural potential difference and intermittent short-circuit current (scc) was made in the rat proximal jejunum in vitro: similarly, potassium and sodium ion movements were measured to investigate the relationship of acidification to electrogenic events and associated ion movements in the jejunum.2. acidification correlated significantly with the short-circuit current and both were inhibited by 10 mm serosal ouabain or 10 mm mucosal amino ... | 1976 | 7670 |
| heterogeneity of cells isolated from rat liver by pronase digestion: ultrastructure, cytochemistry and cell culture. | | 1976 | 7673 |
| potential histamine h2-receptor antagonists. 3. methylhistamines. | syntheses are described for all the mono- and some di- and trimethylhistamines. new methods are given for the known npi, ntau-, nalpha-, 2-, and 4-methylhistamines and for the novel compounds, beta-methyl-, 4,nalpha-dimethyl-, and 4,nalpha,nalpha-trimethylhistamines. agonist activities are reported for stimulation of histamine h1 (guinea-pig ileum) and h2 (rat gastric acid secretion) receptors. h2-receptor agonist activities indicate that a methyl group is more readily accommodated at the 4 and ... | 1976 | 7675 |
| involvement of cyclic nucleotides in the beating response of rat heart cells in culture. | | 1976 | 7680 |
| prolactin in clinical practice. | | 1976 | 7685 |
| the spontaneously hypertensive rat (11). proceedings of xi annual scientific meeting of the council for the spontaneously hypertensive rat (shr) september 27, 1975, tokyo. | | 1976 | 7689 |
| studies on the structure activity relationship of adrenergic beta-mimetic benzylamine derivatives. | appropriately substituted benzylamine (bza) derivatives, fragmented derivatives of tetrahydroisoquinolines, were found to be directly acting adrenergic beta-stimulants, exhibiting tracheal relaxing, positive chronotropic and free fatty acid (ffa) releasing activities. the chemical structures essential for manifestation of the beta-action were i) 3,4-dihydroxybenzylamine, ii) arylmethyl group at position alpha, iii) lower alkyl group on the n atom. the structure activity relationships of bza-deri ... | 1976 | 7693 |
| isohydric regulation of plasma potassium by bicarbonate in the rat. | ph and bicarbonate affect many metabolic reactions but each may change independently. to study bicarbonate's effect onplasma potassium, blood bicarbonate in normal, hypokalemic or hyperkalemic rats was either maintained constant, lowered by hydrochloric acid or raised by sodium bicarbonate administraion. blood ph was maintained constant by changing pco2. in normokalemia lowering bicarbonate increased plasma potassium 2.0meq above values obtained in the other groups. to eliminate urinary potassiu ... | 1976 | 7707 |
| lithium-induced impairment of urine acidification. | the purpose of this study was to clarify the means by which lithium induced a disorder of urine acidification. rats infused with hydrochloric acid (1 meq/kg) developed acute metabolic acidosis (blood ph = 7.32; bicarbonate, 18 meq/liter) with a urine ph of approximately 5.85. the addition of lithium chloride (4 meq/kg i.p) caused an increase in the urine ph (6.38) and a further decrease in blood bicarbonate (11.0 meq/liter). during bicarbonate loading, lithium caused the urine pco2 to fall signi ... | 1976 | 7708 |
| alpha-noradrenergic receptor binding in mammalian brain: differential labeling of agonist and antagonist states. | | 1976 | 7724 |
| rat and mouse tissue-mediated mutagenicity of ring-substituted 3,3-dimethyl-1-phenyltriazenes in salmonella typhimurium. | 3,3-dimethyl-1-phenyltriazene and a series of ring-substituted derivatives (x-phi-n=n-n-(ch3)2:x=substituent(s); phi=phenyl) were tested for their mutagenic and toxic action upon salmonella typhimurium g-46 in a liquid incubation system containing 9000 g tissue supernatants and an nadph-generating system. the compounds could be grouped into four classes according to their toxicity and mutagenicity after 1 h incubation at 37 degrees c at a concentration of 5 mm in the presence of liver supernatan ... | 1976 | 7749 |
| mechanisms of tyrosine hydroxylase and dopamine beta-hydroxylase induction in organ cultures of rat sympathetic ganglia by potassium depolarization and cholinomimetics. | it was the aim of the present study to elucidate the mechanisms involved in specific tyrosine hydroxylase (th) and dopamine beta-hydroxylase (dbh) induction by potassium depolarization and cholinomimetics in rat superior cervical ganglia kept in organ culture. the effect of high (54 mm) potassium concentration on intact ganglia seems to result in a dual action: a) a specific induction of th and dbh via release of acetylcholine from preganglionic cholinergic nerve terminals. b) a non-specific eff ... | 1976 | 7752 |
| anti-dopaminergic and anti-muscarinic effects of dibenzodiazepines: relationship to drug induced parkinsonism. | 1. the anti-dopaminergic effects of several dibenzodiazepines were examined on the dopamine-stimulated adenylate cyclase in rat striatal homogenates. the "cis" isomer of clozapine, hf-2046, was the most potent in this respect and perlapine, which is devoid of neuroleptic activity, was the weakest. 2. the anti-muscarinic effects of the same compounds were measured by using the muscarinic affinity label 3h-propylbenzilylcholine mustard. hf-2046 was the most potent and loxapine the least potent of ... | 1976 | 7753 |
| lack of correlation between changes in cyclic nucleotides and subsequent induction of tyrosine hydroxylase in rat adrenal medulla. | pretreatment of rats with dexamethasone (2.5 mumol/kg, a dose which blocks the release of acth from the pituitary gland) abolished the reserpine mediated increase in camp and the increase in the camp/cgmp ratio in the adrenal medulla. in contrast, the reserpine-mediated induction of tyrosine hydroxylase (th) remained unchanged. hypophysectomy had a similar effect to dexamethasone treatment. since changes in camp and changes in the camp/cgmp ratio are not indispensible prerequisities for the subs ... | 1976 | 7754 |
| the effects of antipyretics on metabolism processes in rat liver mitochondria. part i. the action of sodium salicylate, and pyrazolones on the reaction of respiratory chain. | the effects of sodium salicylate (ss), phenazone (ph) and aminophenazone (aph) on mitochondrial respiration respiration and oxidative phosphorylation were studied in vitro, ss inhibited state 3 but stimulated state 4 of respiration, if alpha-ketoglutarate and succinate were used as the substrates. the inhibition of state 3 of respiration was not reversed by 2,4-dinitrophenol (dnp). ph and aph inhibited the respiratory state 3 without affecting the state 4 in the presence of the same substrates, ... | 1976 | 7775 |
| dietary and hormonal regulation of the content of acetyl coenzyme a carboxylase-synthesizing polysomes in rat liver. | specific polysomes involved the the synthesis of acetyl-coa carboxylase acetyl-coa: carbon-dioxide ligase (adp-forming), ec 6.4.1.2 have been identified by the binding of 125i-labeled antiacetyl-coa carboxylase to rat liver polysomes. the binding is highly specific and occurs through the recognition of the nascent peptide chains on polysomes. with the use of the 125i-labeled antibody binding technique, it has been demonstrated that the relative content of acetyl-coa carboxylase-synthesizing poly ... | 1976 | 7784 |
| gamma-glutamyl transpeptidase, a lymphoid cell-surface marker: relationship to blastogenesis, differentiation, and neoplasia. | gamma-glutamyl transpeptidase, an enzyme that catalyzes gamma-glutamyl transfer from gamma-glutamyl compounds to amino acid and peptide acceptors, and which is known to be localized in the membranes of many epithelial cells, was found in a variety of lymphoid cells. the lymphoid cell enzyme is located on the cell surface, and exhibits substantially the same substrate specificity as the enzyme found in epithelial cells. human and rat (but not mouse) lymphocyte gamma-glutamyl transpeptidase was st ... | 1976 | 7785 |
| protective effect of clofibrate against s. pneumoniae infection in rats. | | 1976 | 7787 |
| new aldehyde dehydrogenase isozymes in chemically induced liver tumors in the rat. | | 1976 | 7788 |
| stress and ulcer etiology in the rat. | | 1976 | 7792 |
| intracranial self-stimulation in rats as a function of various stimulus parameters. vi. influence of fentanyl, piritramide, and morphine on medial forebrain bundle stimulation with monopolar electrodes. | the effects of different subcutaneous doses of fentanyl (0.02, 0.04, 0.08, and 0.16 mg/kg), piritramide (0.63, 2.50, 10.0 and 40.0 mg/kg), and morphine (2.50, 5.00, 10.0 and 20.0 mg/kg) on self-stimulation in rats were studied. different stimulus parameter combinations (spc) inducing low, high or intermediate control response rates (crr) were applied during the same experimental sessions. the three narcotic analgesics induced response depression (rd) and response stimulation (rs). rs was mostly ... | 1976 | 7796 |
| wet dog shake behavior in normal rates, elicited by benzylideneaminooxycarbonic acid derivatives. | wet dog shake (wds) behavior in rats, well known as morphine-withdrawal syndrome, could be elicited without concomitant symptoms for the first time chemically in non-morphine-addicted animals. the capability to produce wds was correlated with a specific chemical structure among the title-compounds. the threshold-dose of the most effective agents was 25-50 mg/kg, rather independent of the mode of application. maximal response of 10-20 wds per min and animal were reached after application of 100-2 ... | 1976 | 7797 |
| hormonal influences of the extinction of conditioned taste aversion. | conditioned taste aversion for a 5% glucose solution (sugar water) was induced in rats by an i.p. injection of licl 30 min after the first presentation of sugar water. extinction of conditioned taste aversion was measured either in the forced-drinking test or in the preference-drinking test. in the forced-drinking test sugar water was the only fluid presented to the animals during extinction sessions. in the preference-drinking test the animals had the choice of tap water or sugar water. the rat ... | 1976 | 7799 |
| methods related to cyclic amp and adenylate cyclase. | | 1975 | 7803 |
| drug inhibition of ecto-atpase and of phagocytosis in leukocytes. | substituted phenothiazines and tricyclic antidepressants which inhibited the ecto-atpase in leukocytes also markedly decreased the phagocytic activity of these cells. in affecting either the enzyme or phagocytosis the order of potency of the drugs was similar. various other cns drugs were ineffective. the results suggest possible involvement of ecto-atpase in the phagocytic process, and indicate adverse effects of the aforelisted drugs on the host defense system against foreign materials, includ ... | 1976 | 7807 |
| induction of tyrosine 3-monooxygenase in adrenal medulla: role of protein kinase activation and translocation. | the transsynaptic induction of tyrosine 3-monooxygenase (th) in rat adrenal medulla is preceded by an early increase in the ratio of cyclic adenosine monophosphate (amp) to cyclic guanosine monophosphate, an activation of cytosol cyclic amp-dependent protein kinase, and a subsequent translocation of protein kinase catalytic subunits from cytosol to subcellular particles. as a result of this translocation, nuclear protein kinase activity increases during the induction of th. transection of splanc ... | 1976 | 7836 |
| calcium-independent modulation of cyclic gmp and activation of guanylate cyclase by nitrosamines. | nitrosamines markedly increase concentrations of guanosine 3', 5' - monophosphate (cyclic gmp) in several tissues from the rat and in human colonic mucosa. these agents are effective in the absence of extracellular calcium and enhance guanylate cyclase activity in tissue homogenates. stimulation of cyclic gmp was greatest in liver, where the carcinogenic activity of nitrosamines is also most pronounced. | 1976 | 7837 |
| isoniazid and iproniazid: activation of metabolites to toxic intermediates in man and rat. | acetylhydrazine, a metabolite of isoniazid, a widely used antituberculosis drug, and isopropylhydrazine, a metabolite of iproniazid, an antidepressant removed from clinical use because of high incidence of liver injury, were oxidized by cytochrome p-450 enzymes in human and rat liver microsomes to highly reactive acylating and alkylating agents. covalent binding of these metabolites to liver macromolecules paralleled hepatic cellular necrosis. the metabolites formed from these and probably other ... | 1976 | 7838 |
| the main pharmacological characteristics of grandaxin (tofizopam, egyt-341). | | 1975 | 7851 |
| morphine-reserpine interactions in the adrenal medulla. | | 1976 | 7860 |
| dinitroorthocresol dissociation of oxidative phosphorylation in the rat liver perfused in situ. | perfusion of the rat liver in situ for 150 min provides for maintaining optimal values of acid-base balance for the following indexes: surplus of bases, content of standard bicarbonate, buffer bases ph, po2, pco2, hbo2, the level of bile secretion, content of lactate, pyruvate, atp, adp, that evidences for a high functional activity in the tissue. introduction of dinitro-ortho-cresol (dnc) into the perfusion liquid causes development of acidosis. dnc results in dissociation of oxidative phosphor ... | 1976 | 7866 |
| lipid metabolism in men served lyophilized rations for 4 to 6 months in a closed biological life support system. | | 1976 | 7880 |
| intracellular ph of the rat heart in chronic hypertrophy. | | 1975 | 7886 |
| induction of a local transplant vs. host reaction by cells of the palatine tonsils. | | 1975 | 7889 |
| alpha-n-benzoylarginine-2-naphthylamide hydrolase (cathepsin bi?) from rat skin. iii. substrate specificity, modifier characteristics, and transformation of the enzyme at acidic ph. | some properties of rat skin benzoylarginine-2-naphthylamide hydrolase types i (preparations i and ai) and ii (preparations ii and nii) were studied. both types were activated by dithiothreitol and edta, but responded differently to 1 mm kcn, when benzoylarginine-2-naphthylamide (bana) was used as a substrate: type i was inhibited, while type ii was activated. when leucine-2-naphthylamide was used as a substrate, both types were activated by kcn. thiol proteinase inhibiting substances, like heavy ... | 1976 | 7911 |
| heterogeneity of hepatic tyrosine aminotransferase. separation of the multiple forms from rat and frog liver by isoelectric focussing and hydroxylapatite column chromatography and their partial characterization. | l-tyrosine:2-oxoglutarate aminotransferase (ec 2.6.1.5; tat) and other enzymes that transaminate tyrosine in rat liver cytosol have been separated into four fractions by isoelectric focussing. one of the forms is probably identical to mitochondrial l-aspartate:2-oxoglutarate aminotransferase (ec 2.6.1.1.; masat). the other three forms have pi's of 4.72, 4.98 and 5.30 and km values of 1.3 and 0.3 mm for tyrosine and alpha-ketoglutarate. these heat stable forms have little or no asat activity. rat ... | 1976 | 7912 |
| the effect of prolonged vasopressin administration on the level and metabolism of catecholamines in the rat brain and kidneys. | dopamine (da) and noradrenaline (na) levels and activities of the enzymes metabolizing catecholamines were determined in the rat brain and kidneys during prolonged (4 weeks) administration of lysine vasopressin (lvp) and 2 weeks after its withdrawal. da level was elevated during the whole period of experiment. na level increased mainly after lvp withdrawal. dopa-decarboxylase activity was elevated in all the experimental animals. tyrosine and dopamine-beta-hydroxylase activities increased at the ... | 1976 | 7918 |
| studies on the antilipolytic effect of acidosis. | | 1976 | 7921 |
| release of amino acids from the spinal cord in vitro and in vivo. | | 1976 | 7928 |
| the transport of metabolizable substances into the living brain. | | 1976 | 7929 |
| inhibition of prostaglandin synthesis in vivo by nonsteroid anti-inflammatory drugs: evidence for the importance of pharmacokinetics. | a variety of acidic and non-acidic compounds are potent inhibitors of prostaglandin (pg) synthesis in vitro. however, only a few, namely the acidic nonsteroid anti-inflammatory drugs (nsaid) are useful anti-inflammatory analgesics in the clinic. since inhibition of pg-synthesis is believed to be the main target of nsaid in inflammation this superiority of acidic compounds remains unexplained. we have considered that one explanation could be that only acidic nsaid appear in high concentrations in ... | 1976 | 7934 |
| quantitation of local acidosis and hypoxia produced by infection. | increased strength of healing incisions infected with e coli was demonstrated in this experiment. efforts to measure respiratory gas tensions and ph in these incisions were unsuccessful. therefore, these moieties were measured in normal and infected wound fluid contained in implanted wire cylinders. the wound fluid from infected cylinders was consistently more acidotic and had a lower po2 and a higher pco2 than fluid from unifected wound cylinders. | 1976 | 7960 |
| effect of chlorpromazine on tyrosine hydroxylase in rat brain striatum and beef adrenal medulla. | | 1976 | 7961 |
| beta-blocking properties compared to other pharmacological and biochemical effects of 1, no2-hydroxyphenyl, n-benzoyl-alanine (gal-1). | | 1976 | 7962 |
| chromatographic separation of cyclic guanosine 3',5'-monophosphate from guanylate cyclase reaction mixtures. | | 1976 | 7970 |
| radioassay of acetyl-coa synthetase, propionyl-coa synthetase and butyryl-coa synthetase in brain. | | 1976 | 7972 |
| fluroxene (2, 2, 2-trifluorethyl vinyl ether) toxicity: a chemical aspect. | fluroxene is highly toxic to several animal species. this toxicity is enhanced by induction of raised levels of hepatic microsomal enzymes. experiments in rats are described which seek to assess the rleative contribution to this toxicity of the individual component groups of the fluroxene molecule. though results point to the trifluoroethyl moiety of fluroxene as that aspect of the molecule most responsible for the observed mortality, reduction of the vinyl group modifies the pattern of liver in ... | 1976 | 7977 |
| fatty acid desaturase system of yeast microsomes. involvement of cytochrome b5-containing electron-transport chain. | | 1976 | 8011 |
| convulsant drugs and inhibitory mechanisms in the mammalian central nervous system. | | 1975 | 8019 |
| inhibition by suprofen and other non-narcotic analgesic drugs of the effects of prostaglandin precursor on isolated tissues and platelets. | contractions caused by slow reacting substance c(srs-c) and by arachidonic acid hydroperoxide (aap) in the guinea-pig ileum and by aap in the rat fundus were studied in the presence of suprofen and of 3 reference compounds. the dose-related inhibitions were not due to antagonism of prostaglandins. other agonists of gastrointestinal smooth muscle were not or only weakly antagonized. for the study of selective inhibition of aap-induced contractions by non-narcotic analgesics, the rat fundus is the ... | 1976 | 8022 |
| discriminative stimulus properties of analgesic drugs: narcotic versus non-narcotic analgesics. | using a food-reinforced two-lever operant procedure, rats (n=6) were trained to discriminate fentanyl (1.25 mg/kg, p.o., t-60') from solvent (1 ml/100 g b.w., p.o., t-60'). the administration of another narcotic analgesic (pethidine) produced a dose-related generalization with the standard fentanyl treatment; six non-narcotic analygesics (suprofen, acetylsalicylic acid, indomethacin, phenacetin, phenylbutazone, tolmetin) were found not to do so. it is concluded that the ability of drugs to produ ... | 1976 | 8023 |
| activities of citrate synthase and nad+-linked and nadp+-linked isocitrate dehydrogenase in muscle from vertebrates and invertebrates. | 1. the activities of citrate synthase, nad+-linked and nadp+-linked isocitrate dehydrogenase were measured in muscles from a large number of animals, in order to provide some indication of the importance of the citric acid cycle in these muscles. according to the differences in enzyme activities, the muscles can be divided into three classes. first, in a number of both vertebrate and invertebrate muscles, the activities of all three enzymes are very low. it is suggested that either the muscles u ... | 1976 | 8036 |
| autophagy-related changes of arylsulphatases a and b in rat liver lysosomes. | the total arylsulphatase activity and the relative activities of lysosomal arylsulphatases a and b were measured in the liver of control rats and rats subjected to treatments that provoke hepatic autophagocytosis. the total liver arylsulphatase activities were increased in starved and starved glucagon-treated rats, but not in sham-operated and hepatectomized rats. arylsulphatases a and b in the mitochondrial-lysosomal (m-l) fraction were separated by polyacrylamide-gel electrophoresis at ph 8.8; ... | 1976 | 8037 |
| the regulation of rat liver tryptophan pyrrolase activity by reduced nicotinamide-adenine dinucleotide (phosphate). experiments with glucose and nicotinamide. | 1. chronic administration of glucose or nicotinamide in drinking water inhibits the activity of rat liver tryptophan pyrrolase, and subsequent withdrawal causes an enhancement. the enzyme activity is also inhibited by administration in drinking water of sucrose, but not fructose, which is capable of preventing the glucose effect. 2. the inhibition by glucose or nictinamide is not due to a defective apoenzyme synthesis nor a decreased cofactor availability. 3. the inhibition by nicotinamide is re ... | 1976 | 8041 |