| idiotypic analysis of lymphocytes in vitro. ii. genetic control of t-helper cell responsiveness to anti-idiotypic antibody. | when the igg1 fraction of anti-idiotypic antibodies raised in guinea pigs is injected into mice, sensitization of idiotypic t and b lymphocytes occurs (1-3). in the present study we analyze the genetic requirements for t-helper cell sensitization by anti-idiotypic antibody. this was done by measuring, in a suitable panel of mouse strains, helper cell responsiveness to two anti-idiotypic reagents which recognize distinct, strain-specific idiotypes, namely the a5a and the s117 marker. whenever hel ... | 1976 | 56419 |
| x-gp70: a third molecular species of the envelope protein gp70 of murine leukemia virus, expressed on mouse lymphoid cells. | three variants of the gp70 envelope component of mulv are now recognizable serologically: gix-gp70, 0-gp70, and x-gp70. the last of these, x-gp70, has so far been found only in mice or cells producing abundant c-type virus. this distinguishes x-gp70, provisionally, from the gix-gp70 and 0-gp70 variants, each of which can be expressed on normal thymocytes without accompanying virus production, as exemplified by mouse strains 129 and b6, respectively. the x-gp70 genotype, however, is not limited t ... | 1976 | 56420 |
| tumor immunity induced by preimmunization with balb/c mouse myeloma protein. | prior sc immunization of balb/c mice with 1 mg isolated m component of mopc-11 mouse myeloma resulted in significant relative immunity to subsequent sc or ip challenge with 10(4) living cells from the same plasmacytoma. however, challenges of 10(5) and 10(6) tumor cells overcame immune status engendered by preimmunization with m component. despite evidence for the specificity of the immunity induced by one isolated m component as opposed to another, no clear cytotoxic antibody, cell-mediated tum ... | 1976 | 56452 |
| purification and serological characterization of the major envelope glycoprotein from akr murine leukemia virus and its reactivity with autogenous immune sera from mice. | the major envelope glycoprotein (gp71) from akr murine leukemia virus (mulv) was purified and its serological reactivity with heterologous and autogenous immune mouse sera was examined. homologous and interspecies competition radioimmunoassays using antisera to rauscher-mulv gp69/71 or friend-mulv gp71 or antisera to feline leukemia virus to precipitate 125i-labeled gp71 from various mulv showed that distinct differences exist between rauscher- or friend-mulv and akr-mulv glycoproteins. characte ... | 1976 | 56459 |
| relationships between intracisternal type a and extracellular oncornavirus-like particles produced in murine mopc-460 myeloma cells. | oncornavirus-like particles of the "a" (both intracisternal and intracytoplasmic) and "b" or "c" (extracellular) types are produced by murine mopc-460 myeloma cells. this communication describes a comparative study on tracisternal a and extracellular particles. both types of particles contain an rna-dependent dna polymerase activity, traces of 35s and 70 s rna in addition to larger amounts of degraded rna, and proteins of approximately 76,000 and 45, 000 daltons. the 76,000-dalton proteins from ... | 1976 | 56464 |
| relationship between the oncornavirus gene product gp70 and a major protein secretion of the mouse genital tract. | | 1976 | 56712 |
| induction of a novel transforming virus (msv-z) from sarcoma-positive leukemia-negative 3t3fl mouse cells. | | 1976 | 56806 |
| [properties of mouse hemocytoblastosis-reticulosis (mazurenko) virus]. | the results of molecular biological study of the properties of hemocytoblastosis-reticulosis virus (mazurenko) are presented. the virus was found to have buoyant density of 1.16 g/cm3 in sucrose density gradient. virions contain 70s rna possess reverse transcriptase activity. | 1976 | 56814 |
| studies on the 14c-histamine release induced by noradrenaline in mouse neoplastic mast cells. | murine neoplastic mast cells incubated with labelled histamine released 14c-histamine when exposed to increasing concentrations of noradrenaline. this process was blocked by low temperature, by inhibiting oxidative metabolism and glycolysis, by calcium deprivation and by cytochalasin b. electron microscope observations of cells exposed to noradrenaline revealed an increase of the juxtanuclear golgi apparatus, and the appearance of a pronounced peripheral vacuolization. cells exposed simultaneous ... | 1975 | 56875 |
| studies on the 14c-histamine release induced by histamine in mouse neoplastic mast cells. | | 1975 | 56876 |
| [on the lacking teratogenic effect of the plasmaexpander hydroxyethyl starch in the rat and mouse (author's transl)]. | hydroxyethyl-starch (hes) has been tested for teratogenic activity in bd-strain rats and swiss mice. hes in both species was shown not to be teratogenic. high doses of 50 g/kg/day given by intraperitoneal injection lead to abortion in all pregnant rats. this report in concerned with the important question of, whether development and normal life of the progeny of rats and mice is affected when treated with hes during pregnancy. special attention has been given to potential teratogenic effects. | 1975 | 56907 |
| localization of rabbit gamma globulins in the mouse visceral yolk sac placenta. | the yolk sac placenta has been implicated previously in transmission of passive immunity to the fetus. this work uses an immunohistochemical technique devised by sternberger et al. ('69) to study this problem. rabbit serum containing gamma globulins was injected into the uterine lumen of the white swiss mouse during the last third of pregnancy. two or four hours later yolk sacs were removed and fixed in 2% paraformaldehyde or freeze-dried and fixed with paraformaldehyde vapors. finely chopped ti ... | 1976 | 56909 |
| removal of inhibitors against rna-directed dna polymerase activity in human milk. | milk from a number of species (e.g., man, mouse, rat, dog, and cow) contains inhibitors of the rna-directed dna polymerase. when attempts are made to isolate virions from the milk, part of the inhibitors follow the virions in the purification. the amount of inhibitors varies in different milk samples. these inhibitors can probably account for the large discrepancies reported in studies of the presence of oncornaviruses in human milk. phosphatases bound to subcellular particles or fragments seem ... | 1976 | 56990 |
| some effects of bleomycin on the proliferation, maturation time and protein synthesis of hairless mouse epidermis. | hairless mice were given 2 mg bleomycin i.p. in 1-0 ml saline on two successive days. by a stathmokinetic method, by micro-flow fluorometry and by autoradiography certain kinetic parameters were measured during 10 days after the last injection. cell counts were made and the turnover time of the differentiating cells estimated. protein synthesis was estimated by the uptake of radioactive histidine, and dry cell mass measured by weighing. bleomycin affected cell proliferation in the epidermis by d ... | 1976 | 57003 |
| stimulated dna synthesis in livers and kidneys induced to proliferate associated with unchanged thymidine and thymidylate kinase activities. | enhanced mitotic activity and stimulated dna synthesis associated with unchanged activity of thymidine and thymidylate kinases were observed in mouse kidneys induced to proliferate by intracardiac injection of lead acetate, and in rat livers following repeated administration of 5-azacytidine. on the other hand, the enhanced thymidine kinase activity evoked by l-tryptophan given by intubation at later stages of liver regeneration was paralleled by the enhanced incorporation of thymidine into dna ... | 1976 | 57007 |
| surface immunoglobulins of lymphocytes in mouse plasmacytoma. iv. evidence for the persistence of the effect of plasmacytoma-rna on the surface immunoglobulins of normal lymphocytes in vivo and in vitro. | the previous findings were confirmed that rna extracted from murine plasmacytoma alters the character of the lymphocyte surface immunoglobulins (sig) to express the idiotypic specificity of the ig of the plasmacytoma from which the rna was derived (cell conversion). rna extracted from spleens of plasmacytoma-rna-injected balb/c mice also had convering activity, and if injected into other mice, caused the appearance of rna active in cell conversion in spleens of the second set of mice. this activ ... | 1976 | 57019 |
| detection of interspecific translocations in mouse-human hybrids by alkaline giemsa staining. | | 1976 | 57063 |
| single cell studies on the antibody-forming potential of fractionated, hapten-specific b lymphocytes. | this study addresses itself to the problem of antibody formation in vitro by mouse splenic b lymphocytes enriched for reactivity to the hapten nip by the hapten-gelatine binding and melting technique of haas and layton (1975). small numbers of nip-gelatine-bound b cells were placed in microcultures either by bulk dispensing of dilute cell suspensions, or by micromanipulation under direct microscopic visualization. antibody formation was induced by the t cell-independent hapten-protein conjugate ... | 1976 | 57094 |
| antigenicity of the m proteins of group a hemolytic streptococci: further evidence for shared determinants among serotypes. | shared antigenic determinants between m proteins of group a streptococci appear to be widespread among serotypes. this is demonstrated by the ability of purified m proteins to absorb opsonic antibody from a variety of heterologous antisera prepared against whole cells or purified m proteins. this absorption procedure has the capacity to separate passive mouse protecting and passive hemagglutinating antibodies from opsonic antibodies measured in vitro. when whole cells or m proteins are used as h ... | 1976 | 57101 |
| delayed hypersensitivity to fungal antigens in mice. ii. molecular classes in immunogenic rna extracts that transfer delayed hypersensitivity. | the transfer of delayed hypersensitivity to coccidioides immitis and candida albicans antigens with immunogenic rna extracts was studied in a mouse model. sensitivity was measured by skin tests and footpad swelling responses. immunogenic rna converted normal spleen cells in vitro so that they produced antigen-specific delayed hypersensitivity in mice that were given injections of the cells. rnase reduced the rate of, but did not abolish, in vitro interaction of immunogenic rna extracts with lymp ... | 1976 | 57198 |
| velocity measurements of particulate neuroplasmic flow in organized mammalian cns tissue cultures. | the movements of spherical particles in the range of 0.3 to 0.8 mum diameter within neurities of cultured embryonic mouse spinal cord fragments were observed and recorded by means of nomarski optics and time-lapse photocinematography at high power. particulate movements were measured by projecting the motion pictures onto a calibrated screen and recording the distances moved with time of linearly moving particles and making note of the direction (toward or away from the neuron soma) of movement. ... | 1976 | 57208 |
| experimental allergic encephalomyelitis (eae) in mice. i. induction of eae with mouse spinal cord homogenate and myelin basic protein. | the condition of experimental allergic encephalomyelitis (eae) induction was investigated in several mice strains. sjl and c3h/he strains were found to be susceptible. a single immunization with mouse spinal cord, complete freund's adjuvant (cfa) and pertussis vaccine produced clinical signs of eae in sjl and c3h/he strains after 11 to 18 days. isogenic spinal cord produced eae in c3h/he strain. a single immunization with myelin basic protein from bovine spinal cord in cfa and pertussis vaccine ... | 1975 | 57253 |
| letter: human lymphocytes and mouse red cells. | | 1976 | 57483 |
| induction of an endogenous b-tropic type c rna virus from swr/j mouse embryo cells in tissue culture. | | 1976 | 57668 |
| nucleotide sequence homologies among mouse mammary tumor viruses. | | 1976 | 57670 |
| the sensitivity to cytotoxic agents of the emt6 tumor in vivo. comparative response of lung nodules in rapid expotential growth and of the solid flank tumour. | experiments are described in which dose-response data have been obtained for emt6 mouse tumour cells growing in vivo and exposed to various cytotoxic agents. a comparison has been made of the response of solid tumours in the flank and of rapidly growing lung nodules. the results are discussed with regard to their cell kinetic implications and compared with our results for the chemosensitivity of emt6 cells in exponential and plateau phase growth in vitro. | 1976 | 57788 |
| the effect of bleomycin and pentamycin in combination on the survival of emt6 mouse tumour cells in vitro and in vivo. | the combined effect of bleomycin and the polyene antibiotic, pentamycin, upon the survival of emt6 tumour cells has been studied both in vitro and in vivo. during growth of the cells as a monolayer in vitro, a very marked potentiation of bleomycin action is seen during the exponential and early plateau phases of growth, but little potentiation occurs in late plateau phase. the effect of exposing the cells to the two drugs consecutively in either order is greater than if the two agents are used a ... | 1976 | 57791 |
| further studies on th-b, a cell surface antigenic determinant present on mouse b cells, plasma cells and immature thymocytes. | | 1976 | 57834 |
| fluorescence analysis of late dna replication in mouse metaphase chromosomes using budr and 33258 hoechst. | | 1976 | 57878 |
| comparative autosomal linkage in mammals: genetics of esterases in mus musculus and rattus norvegicus. | recombination between esterase-4 and esterase-2 in the rat was not observed in 278 backcross offspring. es-4 is thus included within the "esterase cluster" in linkage group v. a new map of this region was constructed and the relationship of the four esterase loci was found to be: es-4-(9.6+/-1.6 cm)-es-2, es-4-(1.5+/-0.7cm)-es-3. homology of this region with a region of linkage group xviii (chromosome 8) of the mouse was proposed on the basis of tissue distribution, subcellular localization and ... | 1976 | 57899 |
| [a study of two non-complementary h-2 mutations in mice using skin transplantation and serologic analysis]. | hz1 and m505 are two mutants affected the kend of h-2 major histocompatibility system in mouse. inability of m505 to complement hz1 is considered as an evidence that the same gene is altered by both mutations. the gained antigens of two mutants can cross-react in vivo, and the lost antigenic determinants are not identical as revealed in skin grafting tests with presensitized recipients. the antiserum asy-0,15, (d x a) anti-i, could differentiate between normal and mutant cells as well as between ... | 1975 | 57901 |
| chemically induced temperature-sensitive mutants of dengue virus type 2: comparison of temperature sensitivity in vitro with infectivity suckling mice, hamsters, and rhesus monkeys. | a series of temperature-sensitive (ts) mutants of dengue virus type 2 (den-2, th-36 isolate) were induced by treatment with 5-azacytidine. these mutants and parental viruses were compared for the ts trait and/or attenuation in four systems: primary hamster kidney cells, suckling mice, golden syrian hamsters, and rhesus monkeys. seven clones judged to possess the ts trait in virto demonstrated a variety of patterns in vivo. on initial isolation, five of seven ts mutants exhibited reduced mouse le ... | 1976 | 57925 |
| specific acquired immune unresponsiveness to contact allergens with cyclophosphamide in the mouse. | treatment with cyclophosphamide (cy) can modulate the acquisition of allergic contact dermatitis in the mouse. we compared the effect of a single dose of cy given at different times before or after allergen. cy one or more days prior to allergen intensified, and cy several days after allergen inhibited the acquisition of sensitivity. mice whose immunological response to an allergen had been suppressed by cy were specifically immunologically tolerant to that allergen, but not to an unrelated alle ... | 1976 | 57929 |
| inhibition by nicotinamide of antigen-induced histamine release from mouse peritoneal mast cells. | the in vitro antigen-induced histamine release from mouse peritoneal mast cells actively sensitized with ige antibodies was inhibited by nicotinamide. the drug was either given in vivo to the sensitized mice (once daily 100 mg/kg) for 7 days before an in vitro experiment or incubated in vitro (in concentrations 1-40 mm) with sensitized mast cells before an antigen challenge. the possible action of nicotinamide on the mechanisms involved in the regulation of antigen-induced histamine release from ... | 1976 | 57931 |
| ability of delayed-type hypersensitivity reactions to distinguish tumor-associated antigens and histocompatibility antigens in soluble extracts from murine fibrosarcomas. | the footpad swelling (fps) test for delayed-type hypersensitivity in the mouse was evaluated for its ability to measure both tumor-associated antigens (taa) and histocompatibility (h) antigens solubilized from methylcholanthrene (mca) induced fibrosarcomas of c57b1/6 (b6) mice. tests for taa were performed in mice immune to syngeneic tumors while h-antigens were assayed in mice immunized with skin allografts. fps was most intense in b6 mice challenged with taa from the immunizing b6 tumor, but a ... | 1976 | 57934 |
| developmental changes of carbonic anhydrase in the retina of the mouse: a histochemical study. | in the optic cup of early mouse embryos, carbonic anhydrase activity is seen only in the pigment epithelial cells. during the late prenatal period the enzyme appears in the müller cells, first in the perikarya. during the postnatal development the enzyme activity appears in the radial processes of the müller cells starting at the vitreal border along with the separation of the outer retinal layers. this is followed by increased activity in the plexiform layers, presumably in the lateral processe ... | 1976 | 57949 |
| a comparison of two methods of preparing cells or nuclei for high-resolution microspectrophotometry. | suspensions of isolated mouse thymus cells were subjected to two preparative methods: either they were dropped through several mm of 9:1 v/v ethanol-acetic acid fixative, allowed to stand for 1hr and then processed for staining; or they were fixed, passed through a graded ethanol series to 70% ethanol, centrifuged on to slides in a modified shandon cytocentrifuge and then carried wet into the staining procedure. all preparations were stained by the feulgen reaction and evaluated by high-resoluti ... | 1976 | 57951 |
| purification and characterization of a liver-specific antigen. | a liver-specific antigen (f-antigen) previously demonstrated in saline extracts of balb/c mouse liver by double immunodiffusion was isolated and characterized. the antigen was found widely distributed among mammals but absent from avian and frog liver extracts. in immunoelectrophoresis it had an electrophoretic mobility similar to that of serum beta2-globulins, was relatively thermolabile, and was precipitated at 30 to 70% saturated ammonium sulfate concentrations. evidence was presented that th ... | 1976 | 58026 |
| antigen-specific and nonspecific mediatiors of t cell/b cell cooperation. ii. two helper t cells distinguished by their antigen sensitivities. | further evidence is presented for two types of helper t cells in the mouse specific for the protein antigen, keyhole limpet hemocyanin (klh). the first cell helps b cells respond to the trinitrophenyl hapten (tnp) coupled to klh, is primed by relatively high doses of antigen in vivo, and yet the effector cell is stimulated by very low doses of antigen in vitro. the second cell helps b cells respond to a non-cross-reacting antigen, sheep red blood cells, presumably via production of a nonspecific ... | 1976 | 58030 |
| anti-immunoglobulin stimulation of murine lymphocytes. i. age dependency of the proliferative response. | the in vitro proliferative response of normal mouse spleen cells to anti-immunoglobulin (anti-ig) reagents was found to be an age-associated phenomenon. the response usually appears in mice when they reach 5 to 7 months of age and is rarely seen in younger animals. anti-ig induced proliferation was observed by using two different antisera--one polyvalent, prepared against mouse antibody-antigen complexes and one prepared against mouse igm myeloma. both antisera were shown to be specific for b ce ... | 1976 | 58043 |
| maintenance of plasma-derived proteins at much lower concentrations in the uterine lumen of the rabbit: a kinetic study of passage. | human serum albumin (hsa) and human gamma globulin (hgg) in serum and uterine fluid of nonpregnant rabbits at various times after an i.v. injection (100 mg/kg) were measured by a radial immunodiffusion test using specific antisera. the hsa concentration in uterine fluid rose to a peak at 12 hr when it was 11% of the serum concentration and then declined, whereas hgg reached a peak at 18 hr (3.2% of serum level) and decreased thereafter. the hsa passed 2 1/2 times faster than hgg, but both protei ... | 1976 | 58060 |
| high-resolution autoradiography as a tool for the localization of nucleic acid synthesis and distribution in the mammalian cell nucleus. | several examples of the application of high resolution autoradiography to the study of nucleic acid distribution in ultrathin sections of fixed and plastic embedded or frozen material are presented. newly-synthesized dna, labelled by very short pulses of 3h-thymidine is found to be localized throughout the nucleus. in the blastomeres of early mouse embryos developing after fertilization by 3h-thymidine-labelled spermatozoa, the labelled paternal dna is distributed non-homogeneously in the nucleu ... | 1976 | 58064 |
| identification of peroxisomes (microbodies) in mouse myocardium. | | 1976 | 58065 |
| endogenous ecotropic mouse type c viruses deficient in replication and production of xc plaques. | endogenous ecotropic type c viruses were induced by iodedeoxyuridine from nontransformed and chemically or spontaneously transformed clones of the c3h/10t1/2 cell line. viruses produced by cells of certain transformed clones were n-tropic and formed large xc plaques. in contrast, viruses produced by nontransformed c3h/10t1/2 cells were not detectable in the xc plaque test. these xc- viruses infected mouse cells with high efficiency, as shown by the induction of murine leukemia virus group-specif ... | 1976 | 58071 |
| radiommunoassays for the 70,000-molecular-weight glycoproteins of endogenous mouse type c viruses: viral antigen expression in normal mouse tissues and sera. | genetic information coding for type c rna viruses is transmitted within the dna of mouse cells. at least three endogenous viruses have so far been immunologically distinguished by radioimmunoassays for their 12,000-molecular-weight polypeptides (p12). in the present study, the 70,000-molecular-weight glycoproteins (gp70) of three prototype viruses were purified, and competition radioimmunoassays were developed for each. by use of these immunoassays, the antigenic determinants of gp70's of differ ... | 1976 | 58081 |
| x-ray-induced chromosome aberrations in mouse dictyate oocytes. i. time and dose relationships. | structural chromosome aberrations were analyzed in superovulated metaphase-i oocytes of the mouse, mus musculus, at various times after a single acute dose of 200 r of x-rays. the aberrations seen were of the chromatid type, i.e., chromatid interchanges, isochromatid deletions and chromatid deletions. the aberration frequency was low during the interval 24 h to 5 days between irradiation and ovulation; peak frequency was reached when irradiation was given 14 days prior to ovulation. a dose-respo ... | 1976 | 58377 |
| formation and characteristics of reovirus subviral particles in interferon-treated mouse l cells. | we examined the early phase of reovirus replication in interferon-treated l cells. no difference was detected in the adsorption of virus to cells, the rate and extent of conversion of parental virions to sub-viral particles (svps), and the protein and double-stranded rna composition of the svps when comparing reovirus infection of the interferon-treated and control cells. furthermore, when tested in vitro, svps isolated from interferon-treated cells (svpint) synthesized and methylated reo mrnas ... | 1976 | 58408 |
| ia antigens and fc receptors. | the function of fc receptors (fcr) on mouse spleen cells as detected by a sensitive rosetting system using antibody-labeled bovine erythrocytes as indicator cells was shown to be inhibited by anti-ia antiserum. furthermore, the ia specificities of each i-region sublocus (i-1a, i-1b, i-c) seem to be associated with frr, since fcr function was also inhibited when the blocking anti-ia antiserum recognized only restricted ia specificities. antiserum directed against antigens coded for by the k-end o ... | 1976 | 58439 |
| purification and properties of murine mammary tumor virus dna polymerase. | | 1976 | 58470 |
| removal of inhibitors of particulate reverse transcriptase in human milk. | | 1976 | 58567 |
| biology of isolate immunocytes. iii. fine immunocytological characterization of lymphoplasma cells. | a system for characterization of lymphoplasma cells based on ultrastructural, immunocytological and autoradiographic data is proposed. it is composed of six cell types and transitional forms. any mouse lymphocyte can be classified within one of these forms and the lymphocytes of a lymphoid organ may be subdivided into populations of characteristic cells. the cell types are defined as follows: 1) the differentiated ig lymphocytes which bear ig at their surface, have a rough nuclear sap, a granula ... | 1975 | 58576 |
| ultrastructural studies on the external surface coat of epidermal cells: alcian blue-lanthanum nitrate staining during wound healing. | the external surface coat on mouse epidermal cells has been studied during epidermal wound healing in suction-induced intact subepidermal blisters. by sequentially incorporating the cationic dye alcian blue and the electron opaque tracer lanthanum nitrate into the primary and secondary fixatives for electron microscopy (ab/ln staining), we have demonstrated a selective staining of the epidermal surface coat. at 12 and 24 h after wounding, ab/ln staining always occurred on opposing cell membranes ... | 1976 | 58582 |
| [composition of lipids and proteins of two compounds isolated from infected mice: mouse mammary tumor virus and milk fat globule membrane]. | | 1975 | 58612 |
| a reappraisal of sudan black staining of enamel matrix in the mouse molar. | | 1976 | 58655 |
| endogenous c-type viruses: double agents in natural life processes. | c-type rna viruses have been described in about 20 different vertebrate species. their presence in man is suggested by electron microscopic, biochemical and serologic studies, although a definite re;icating human virus has not yet been isolated. these viruses are inherited through the germ cell and their production is regulated by genetic information carried in the host cell (i.e. endogenous virus). two classes of endogenous c-type viruses have been recognized in certain animals particularly the ... | 1976 | 58679 |
| intracellular distribution of alpha-fetoprotein and albumin messenger rnas in developing mouse liver. | intracellular distribution of active mrnas for alpha-fetoprotein (alphafp) and albumin in fetal and adult mouse liver was studied. livers were fractionated into nucleus and cytoplasm. the latter was further fractionated by sucrose density-gradient centrifugation into four subfractions, the top fraction containing soluble components, the 1.0 m sucrose layer containing primarily 80s ribosomes, the 1.5 m sucrose layer containing light polysomes and the pellets containing heavy polysomes. rna was ex ... | 1976 | 58703 |
| temporary interruption of regional blood flow combined with local hyperthermia for cancer chemotherapy. | a striking chemotherapeutically curative effect on tumor was obtained by means of temporary interruption of regional blood flow combined with local hyperthermia. by analyzing various basic conditions required for this system using ehrlich tumor implanted in the hind limbs of mice, the following were found to be essentially indispensable to obtain satisfactory chemotherapeutic effects: (a) a time interval of 1 to 3 min after systemic i.v. administration of drug to the mice, (b) use of a tournique ... | 1976 | 58714 |
| intracellular distribution of (14c)bleomycin and the cytokinetic effects of bleomycin in the mouse tumor. | the differential effects (blm) on cycling and noncycling cells were investigated with a mouse ascites tumor in vivo. an i.p. injection of 37.0 or 111.1 mug blm per g caused a decrease in tumor cell number but an increase in percentage of tumor cells in mitosis. there are no significant differences between the percentage labeled mitoses at various times after pulse labeling by tritiated thymidine of blm-treated tumor cells and by that of an untreated control, except that the height of the second ... | 1976 | 58717 |
| inhibition of mammalian and oncornavirus nucleic acid polymerase activities by alkoxybenzophenanthridine alkaloids. | the alkoxybenzophenanthridine alkaloids (coralyne acetosulfate, fagaronine chloride, and nitidine chloride) have been reported to possess antileukemic activity in mice. these compounds were tested for inhibition of reverse transcriptase activity of an rna tumor virus and dna polymerase, rna polymerase, and polyadenylic acid polymerase activities of nih-swiss mouse embryos. reverse transcriptase and dna polymerase activities were strongly inhibited by these antileukemic alkaloids, whereas rna pol ... | 1976 | 58719 |
| detection of nervous-system specific cell surface antigen(s) by heterologous anti-mouse brain antiserum. | | 1976 | 58733 |
| antigen-specific t-cell factor in cell cooperation and genetic control of the immune response. | cell interactions between thymus-derived (t) and bone marrow-derived (b) lymphocytes in the antibody response appear to involve soluble t-cell mediators known as 'factors.' this paper describes the properties of a t-cell factor that has specificity for the inducing antigen, a synthetic polypeptide (t, g)-a--l, and is able to replace t cells in the thymus-dependent antibody response to (t, g)-a--l. besides antigen specificity, the main features of the molecule are that it is nonimmunoglobulin; it ... | 1976 | 58799 |
| f-antigen: nature, liver specificity, and release in experimental liver injury. | f-antigen is a liver-specific antigen detected with antibody, raised in allogeneic (cba) mice, to balb/c mouse liver extract. the authors have confirmed the hepatic specificity of this antigen by showing it to be absent in extracts of extrahepatic organs of mouse and human origin. it is present in liver extracts of guinea pigs, rats, and rabbits, as well as in liver extracts from mouse and human sources. the antigen was present in the circulation of rabbits with acute carbon tetrachloride-induce ... | 1976 | 58803 |
| antigen-non-specific t-cell factor in b-cell activation. origin, biological properties and failure to show a relationship to h-2. | a population of t cells sensitive to als treatment release a non-specific factor (nsf) capable of replacing t cells in the response of nude spleen cells to erythrocyte antigens. this factor cannot be removed by immunoadsorbents with specificities directed towards products of the h2 complex, nor by lentil lectin, unlike certain specific t-cell factors. furthermore, it functions across histocompatibility differences between mouse strains. it has been directly demonstrated that this factor can infl ... | 1976 | 58834 |
| an improved method for isolation of h-2 and ia alloantigens with immunoprecipitation induced by protein a-bearing staphylococci. | mouse alloantigens, including the histocompatibility (h-2) and immune response linked (ia) antigen molecules, can be readily isolated by a new precipitation technique utilizing protein a-bearing, fixed staphylococci. the antigens are prepared by radiolabeling mouse spleen cells with 3h-leucine, solubilizing with the non-ionic detergent non-idet p-40, and purifying by affinity chromatography with lentil lectin coupled to sapharose. the antigen preparations are mixed with appropriate alloantisera, ... | 1976 | 58931 |
| blocking of mlc stimulation by anti-ia sera: studies with the virus plaque assay. | the development of congenic mouse strains identical at the h-2k and h-2d loci but differing by i-region associated (ia) determinants has permitted an association to be established between ia determinants and stimulation in mixed lymphocyte culture reactions (mlr). the present experiments were undertaken to establish whether the ir-coded control of mlr operated at the level of recognition or of stimulation. reciprocal mlr were established between a.th and a.tl mouse spleen cells in the presence o ... | 1976 | 58932 |
| immunoenzymatic methods for the study of surface bound immunoglobulins in mice. | methods are described for the study of lymphocyte surface bound immunoglobulins (ig) for light microscopy experimentation. a) live peripheral lymph node cells (lnc) or spleen cells were reacted in suspension under capping conditions with rabbit anti-mouse ig antibodies. after washing the cells were cytocentrifuged, fixed and relabeled with either peroxidase-(po) of fluorescein-(fl) conjugated anti-rabbit igg antibodies (method sc). the staining was heterogeneous and three main categories of lymp ... | 1976 | 58945 |
| chromosomal assignment of the hl-a common antigenic determinants in man-mouse somatic cell hybrids. | in the study presented here, man-mouse somatic cell hybrid clones were examined by means of radioimmunoassays for the presence of both beta2-microglobulin (beta2m) and the hl-a xenoantigenic determinant. in addition, the clones were examined for their karyotype and the expression of enzymes with known chromosomal assignments. the results obtained indicate that the gene coding for the hl-a xenoantigenic determinant is carred on chromosome 6. the data obtained provides a direct demonstration that ... | 1976 | 58947 |
| a mouse plasma substance carrying beta2-microglobulin activity and lacking in h-2 alloantigenic activity. | a mouse plasma substance carrying beta-2-microglobulin activity and lacking in h-2 alloantigenic activity was separated from the blood plasma of a/j strain female mice. the plasma substance had a molecular size of about 300,000-400,000 daltons and an electrophoretic mobility of alpha-globulin. the plasma substance was split by papain digestion to a fragment of about 50,000-60,000 daltons that still carried beta2-microglobulin activity. the papain-split plasma substance was devoid of h-2 alloanti ... | 1976 | 58948 |
| differential sensitivity of rauscher murine leukaemia virus (mulv-r) to interferons in two interferon-responsive cell lines. | the effect of interferon on the replication of vesicular stomatitis virus (vsv) and type-c oncornavirus in two balb/c mouse cell lines, jls-v5 and jls-v9r, infected with mulv-r was examined. vsv replication was inhibited threefold (0-5 log10) in both cell lines by 10 to 20 units of interferon/ml. in jls-v5 cells c-type virus yields, as measured by 3h-uridine incorporation and reverse transcriptase activity, were also reduced threefold by 10 to 20 units of interferon/ml. however, in jls-v9r cells ... | 1976 | 58965 |
| immunocytochemical localization of mouse alpha 2-macroglobulinlike antigenic determinants on schistosoma mansoni adults. | antiserum prepared in rhesus monkeys against purified mouse alpha 2-macroglobulin (alpha2m) was labeled with peroxidase and incubated with both living and formalin-fixed s. mansoni adults (perfused from mice or rhesus monkeys) in order to test for the presence of mouse alpha2m antigenic determinants on their surfaces. following standard cytochemical processing with the appropriate controls, adult worms of both murine and primate origin were found to have mouse alpha2m-like determinants on their ... | 1976 | 58985 |
| "normal" elimination of aberrant autoimmune clones. | tetraparental mouse chimaeras formed by aggregation of early embryos have been utilised in the study of immunological mechanisms. tolerance in chimaeras has proven somewhat controversial, but recent evidence has suggested the existence of "classic" tolerance and furthermore that this is achieved by elimination of cells potentially capable of fatal self in equilibrium self reactivity. clonal elimination was also considered to be responsible for the "correction" of the autoimmune disease of nzb mi ... | 1976 | 59078 |
| protection of cattle against experimental haemorrhagic septicaemia by the capsular antigens of pasteurella multocida, types b and e. | aluminum hydroxide adjuvant vaccines containing endotoxin-free capsular antigens of pasteurella multocida, types b and e, were administered to cattle. dose dependent serological responses were observed which were similar for both antigens. the immunised cattle were subjected to intravenous challenge by a virulent type e strain. all animals which received the highest vaccine dose survived and all unimmunised control animals died and a vaccine dose-response relationship was obtained. the results o ... | 1976 | 59379 |
| a simple staining method for the analysis of meiotic metaphase ii divisions in the male mouse. | | 1976 | 59415 |
| an improved polychrome staining method for thick epoxy sections. | improved polychrome staining of 1-1.5 mum epoxy sections is achieved with sequential applications of a single basic fuchsin-methylene blue mixture at two different ph values. the dye solution is applied for 2-3 min at 50-52 c first at ph 7.9, then at ph 6.7. in sections of mouse mammary tissue, epithelial cells are stained deep blue, connective tissue pink, and fat cells bright olive-green. this simple technique consistently yields uniform, vivid, contrasting colors that sharply delineate the el ... | 1976 | 59424 |
| partial amino acid sequence of mouse beta2-microglobulin. | | 1976 | 59595 |
| induction of particle-bound renin and arginine esterase by testosterone in the mouse. | | 1976 | 59599 |
| [linear- and areal-analytical results of the karyometric studies on monolayer cultures and the influence of actinomycin (author's transl)]. | actinomycin d (amd) inhibits the rna-synthesis by intercalating with the dna-helix. in monolayer culture of embryonic mouse brain cells in the 8th passage the cell nuclei were demonstrated with the feulgen-reaction and quantitatively-morphometrically evaluated following a 48 h intoxication with amd (0.001; 0.01; 0.1 mug/ml). the mean value of nuclear area calculated from single measurements with the quantimet 720 is significantly larger when compared to the controls at a dose of 0.01 mug amd/ml. ... | 1976 | 59603 |
| purification and translation of an immunoglobulin lambda chain messenger rna from mouse myeloma. | here we describe the 500-fold purification of an mrna encoding an immunoglobulin lambda light chain derived from the mouse myeloma tumor, rpc-20. purification involves the isolation of membrane-bound polysomes, oligo(dt)-cellulose chromatography, and sucrose gradient centrifugation under conditions favoring denaturation of polynucleotide complexes. the mrna purified in this way directs the cell-free synthesis of a polypeptide which is five or six amino acids longer than the mature form of rpc-20 ... | 1976 | 59605 |
| immunofluorescence analysis of ig determinants of mouse thymocytes and t cells. | | 1976 | 59697 |
| the use of 3h serotonin release from mast cells of the mouse as an assay for mediator liberation. | the release of 3h 5-ht from murine mast cells is shown to be a simple reproducible method for studying the activation of such cells by various agents. 3h-serotonin was taken up by peritoneal cell suspensions in vitro and was released by antigen, concanavalin a, forssman antiserum, anti-mouse immunoglobulin, or a polypeptide antibiotic, cinnamycin. | 1976 | 59700 |
| antigenic relationships on the diphtheria toxin molecule: antitoxin versus antitoxoid. | we used the mouse to produce antisera to native diphtheria toxin and diphtheria toxoid. with these antisera it was possible to distinguish between toxin and toxoid. by gel diffusion analysis, antitoxin detected antigenic determinants on toxin which were not available on toxoid, indicating that some determinants had been lost or altered by formalin treatment. antitoxoid, on the other hand, showed reactions of identity between toxin and toxoid in gel diffusion. the toxin neutralization titers meas ... | 1976 | 59703 |
| lysis and necrosis: analysis of two cytotoxic phenomena mediated by lymphocytes. | stimulation of rat lymphocytes by allogeneic and xenogeneic (mouse) embryo fibroblast monolayers or by pokeweed mitogen (pwm) generate two different cytotoxic manifestations: a) cell-mediated target cell lysis obtained by contact between effector cells and target cells; b) cytotoxicity caused by lymphotoxin (here termed "necrosis"). activation of hypersensitive lymphocytes by soluble antigen, e.g., keyhole limpet hemocyanin (klh), produces only necrosis but no lytic effect. this difference betwe ... | 1976 | 59715 |
| [ultrastructural identification and immunocytochemical study of somatostatin cells in antral mucosa of the rabbit and the mouse (author's transl)]. | in normal and l-dopa treated rabbits and mice, combined immunochemical methods, photonic histological methods for endocrine cells and ultrastructural methods were used to elucidate ultrastructure and properties of somatostatin cells of the antral mucosa. in normal rabbits, immunoreactive cells giving no fluorescence with falck's technic, they corresponded neither to serotonin cells nor gastrin cells; they were unreactive with fontana, hellerström-hellmann, sevier-munger and mac conaill methods b ... | 1976 | 59723 |
| cross-reactive t-cell antigens among mammalian species. | fluoresceinated heteroantisera prepared against t cells of rats, monkeys, and humans were reacted withthymus and spleen cells from 11 selected species. these reagents recognized cross-reacting t cell antigen(s) among rodent species (mouse, rat, guinea pig, and hamster) and among primate species (monkey and humans). with one exception, the cross-reactivity was restricted to a phylogenetic order. all three antisera required relatively few absorptions to achieve t cell specificity for related speci ... | 1976 | 59778 |
| immune response of the mouse to the major core protein (p30) of ecotropic leukemia viruses. | sera from normal c57bl/6 mice contained low titers of antibodies against proteins of mulv. sera from c57bl/6 mice that were immunized with allogeneic leukemia cells sometimes contained high-titered antibodies against the p15 protein of mulv; these antibodies detected group-specific antigenic determinants of the p15 protein, since reactions were observed with the p15 proteins of both akr and moloney viruses. in contrast, antisera prepared in c57bl/6 mice against the akr leukemia k36 reacted stron ... | 1976 | 59785 |
| serum-soluble malarial antigens and immune complex nephritis in plasmodium berghei berghei infected mice. | swiss albino mice infected with plasmodium berghei berghi showed the serum-soluble malarial antigen and antibody on day 10 of infection onward. immune complex nephritis in these mice developed on the seventh day after inoculation. the infected kidneys revealed the deposition of mouse gamma globulin, mouse beta1c globulin and malaria antigen along the capillary wall of the glomeruli. proteinuria was detected on seventh day of infection. serum-soluble malaria antigen in probably responsible for fo ... | 1976 | 59812 |
| naturally occurring murine leukemia viruses in wild mice: characterization of a new "amphotropic" class. | a new class of murine leukemia viruses, isolated from wild mus musculus trapped in california, is described. these viruses, designated "amphotropic," replicate in mouse, rabbit, mink, human, guinea pig, and rat cells, but not in hamster, quail, or duck cells. they show n-tropism for mouse cells, and do not trigger the xc cell response. they are distinct by interference and virus neutralization testing from the previously recognized mouse-tropic and xenotropic mulv classes. mouse-tropic viruses o ... | 1976 | 59816 |
| effect of bacterial lipopolysaccharides on anti-trinitrophenyl antibody-producing cells. nonspecific modification of the affinity at the cellular level. | the effect of lipopolysaccharide (lps) on anti-trinitrophenyl (tnp) direct plaque-forming cells (pfc) in the spleen of mice and the affinity of antibodies produced by these pfc were examined. simultaneous injection of bacterial lps and tnp-coupled sheep red blood cells(srbc) induced an obvious increase in anti-tnp pfc numbers and heightened the antibody affinity at cellular levels. the higher the doses of lps, the greater the effects. concomitant injection of lps in tnp-coupled homologous mouse ... | 1975 | 59829 |
| complex nature of the production of 'effector' cells and their interaction with target cells in the m-antigen system of the mouse. | the capacity of lymphocytes from mouse strain cba to generate 'effector' cells against the h-2-compatible, m-antigen-incompatible strain c3h and their interaction with such target cells were investigated. it was observed that cba lymphocytes injected in, and 5 days later obtained from, the spleens of irradiated c3h x cba hybrids ('educated cells') could strongly inhibit the growth of c3h x cba bone marrow cells but were almost nonresponsive to c3h x c57bi bone marrow targets (h-2-incompatible). ... | 1976 | 59943 |
| the use of methyl green-pyronin staining after glutaraldehyde fixation and paraffin or araldite embedding. | methyl green-pyronin staining has been used for localization of rna and dna in chick and mouse embryonic tissues and in insect larval salivary glands. glutaraldehyde or tricholoracetic acid-lanthanum acetate (tca-la) was used as fixative and paraffin wax or araldite was used as embedding medium. for good results the following are specially desirable: fixation with 2.5% glutaraldehyde, dehydration in alcohols for short time, and the use of fresh staining solutions. after tca-la fixation the final ... | 1976 | 59965 |
| presence of beta2-microglobulin on the implanting mouse blastocyst. | | 1976 | 59985 |
| action of anti-mouse ige on mouse and rat mast cells. | the action of anti-mouse ige on normal or sensitized mouse and rat peritoneal mast cells as well as on the reaginic activity of mouse and rat antisera was studied. anti-mouse ige induced histamine release from normal mouse peritoneal mast cells in vitro and this effect was greatly enhanced when mast cells were actively sensitized. the action of anti-mouse ige on normal rat peritoneal mast cells was slight; it was enhanced after sensitization of these cells with mouse reaginic antibodies as well ... | 1976 | 60036 |
| the pharmacology of butorphanol, a 3,14-dihydroxymorphinan narcotic antagonist analgesic. | butorphanol, a new, totally synthetic morphinan, which is chemically related to naloxone, has been demonstrated to have both analgesic and narcotic antagonist properties. in rodent antiwrithing analgesic tests, butorphanol was 4 to 30 times more potent than morphine and dl-pentazocine, respectively. as an antagonist, butorphanol was about equivalent to nalorphine and 30 times more potent than dl-pentazocine. on the basis of the naloxone-induced mouse jumping test and the lack of substitution in ... | 1976 | 60089 |
| a histological stain for detecting friend leukemic cells in fixed hemopoietic tissue. | a histological stain described by menzies in 1963 has been useful in detecting early evidence of erythroleukemia (friend disease) in mouse hemopoietic tissues fixed in formaldehyde solution. with the use of this stain, one can distinguish erythroblastic leukemia cells from lymphoblastic leukemia cells. | 1976 | 60094 |
| failure of 5-ethyl-2'-deoxyuridine to induce oncogenic rna (oncorna) viruses in fischer rat embryo cells and in balb/3t3 mouse cells. | | 1976 | 60106 |
| anti-streptococcal antibodies reacting with brain tissue. ii. utrastructural studies. | the reaction of rabbit anti-streptococcus pyogenes type 24 with mouse brain has been studied at the ultrastructural level using horseradish peroxidase labelled antiglobulin. it is shown that these antibodies, and by inference those found in sydenham's chorea, react with the fibrous astrocyte. the cross-reacting antigen is the glial filament or a closely associated cytoplasmic constituent. | 1976 | 60120 |
| immunochemical studies of proteins in epidermal cornified cells of human and newborn rat. | 1. proteins were extracted from cornified cells of newborn rats and human palm with 8 m urea containing 0.1 m beta-mercaptoethanol. two fractions, rat fiiia and human f5.5 were obtained by acid precipitation for further study. 2. antibodies raised in rabbits by injection of rat fiiia gave two precipitin lines by agarose diffusion against rat fiiia, but only one line against human f5.5. one of the antigenic determinants of rat fiiia was found to be a protein of approximately 66 000 daltons. the o ... | 1976 | 60135 |
| [interaction of anti-kb and anti-dd effector lymphocytes with target cells of mouse mutant haplotypes]. | the magnitude of the cytotoxic effect (ce) of immune lymphocytes r101 anti-kb (c57bl/6) and r101 anti-dd (b10.d2) upon target cells (tc) of mutants kba (hzl) and kbd (m505), as well as dda (m504) was considerably less than that on the tc of the original c57bl/6 and b10.d2 strains, respectively. a decrease of the ce on the h (zl) and m504 tc was more prominent than that on the m505 tc. "monospecific" lymphocytes anti-kb and anti-dd were separated into two distinct subpopulations by absorption on ... | 1976 | 60153 |
| [antigenic structure of phytohemagglutinin-stimulated mouse lymphocytes using specific antisera]. | the use of antiserum to the intact and the pha-transformed lymphocytes showed that a new antigenic determinant (or determinants) appeared on the surface of lymphocytes after 68-hour pha-stimulation. intact lymphocytes or cells stimulated by the pha for 2 hours only didn't carry this antigenic marker. at the same time the quantity (or density) of the antigenic markers present on the surface of the intact lymphocytes was lowered in the lymphocytes stimulated with the pha for a long time. | 1976 | 60155 |
| the subcellular localization of calcium in vertebrate smooth muscle; calcium-containing and calcium-accumulating structures in muscle cells of mouse intestine. | the intracellular localization of calcium by means of cytochemical techniques was studied in smooth muscle cells of mouse intestine. when the lead acetate method according to carasso and favard (1966) was used calcium was found in mitochondria and sarcoplasmic reticulum and occasionally between the myofilaments. the active atp-dependent accumulation of calcium into cell structures was investigated by the oxalate method (heumann and zebe, 1967). after appropriate treatment the only structures of ... | 1976 | 60177 |