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meningoencephalitis associated with sarcocystis spp. in a free-living japanese raccoon dog (nyctereutes procyonoides viverrinus).a free-living, young adult, male japanese raccoon dog (nyctereutes procyonoides viverrinus) was rescued in gifu, japan in march 2009. the animal was weak and emaciated with neurological signs that included head tilt, tremor and tic. the brain showed no gross abnormality at necropsy, but microscopically there was severe meningoencephalitis associated with protozoa, which were morphologically consistent with the asexual developmental stage of sarcocystis spp. the protozoa were immunohistochemicall ...201020163802
epidemiologic and zoonotic aspects of ascarid infections in dogs and cats.toxocaracanis and toxocara cati of dogs and cats, respectively, can cause significant disease in people. human seroprevalence for toxocara antibodies varies with factors such as geographic location, socio-economic status, and dietary habits. risk factors for infection include geophagia and low-level education. toxocara canis is better recognized as a cause of human toxocariasis, but toxocara cati should not be overlooked. in addition, patent infections with baylisascaris procyonis, the raccoon a ...201020172762
[non-indigenous and non-specific parasitic nematodes--the background and the consequences].introduction of the host is the main reason behind introduction of the parasite, e. g., nematodes. the introduction of the japanese eel (anguilla japonica) in europe in the 1980s resulted in expansion of the swimbladder nematode anguillicola crassus which soon invaded not only populations of the european eel (a. anguilla), but also other local fish. the raccoon (procyon lotor) is native to north america. it is the specific host of strongyloides procyonis and baylisascaris procyonis. more than 90 ...200920209803
[baylisascariasis--a new dangerous zoonosis].baylisascaris procyonis is a large nematode of the order ascaridida, specific for raccoon (procyon lotor). in north america, raccoons are extremely common in rural, suburban, and urban settings, where they have become well adapted to living alongside people. in the 1930s raccoons were introduced into europe (i. a. poland) and asia for the commercial fur trade and into japan as pets. the prevalence of b. procyonis infection in raccoons is often high, and infected animals can disseminate in their ...200920209804
the important zoonoses in the protected areas of the tatra national park (tanap).the northern part of slovakia constitutes an important tourist and recreational area of the country. protected localities of the tatra national park (tanap) are characterised by specific ecological conditions. the high numbers of animals inhabiting protected areas of the tanap and their potential encounters with tourists contribute to a risk of transmission of important parasitic zoonoses. the aim of presently reported study was to perform a long-lasting and detailed survey on the occurrence of ...200920209814
insecticides to control the european corn borer in field corn. 194720264508
infestation of a raccoon by amblyomma americanum. 194720264533
antibody responses of raccoons naturally exposed to influenza a virus.an investigation was performed to describe the responses of naturally acquired antibodies to influenza a virus in raccoons (procyon lotor) over time. seven wild raccoons, some of which had been exposed to multiple subtypes of influenza a virus, were held in captivity for 279 days, and serum samples were collected on 10 occasions during this interval. serum samples from 9 of 10 bleeding occasions were tested using an epitope-blocking enzyme-linked immunosorbent assay for the presence of antibodie ...201020370429
raccoon roundworm encephalitis.raccoon roundworm encephalitis is a rare but devastating infection characterized by progressive neurological decline despite attempted therapy. patients present with deteriorating neurological function, eosinophilia, and history of pica or geophagia resulting in ingestion of the parasite. neuroimaging studies demonstrate nonspecific findings of progressive white matter inflammation and cortical atrophy.201020411256
estimating contact process saturation in sylvatic transmission of trypanosoma cruzi in the united states.although it has been known for nearly a century that strains of trypanosoma cruzi, the etiological agent for chagas' disease, are enzootic in the southern u.s., much remains unknown about the dynamics of its transmission in the sylvatic cycles that maintain it, including the relative importance of different transmission routes. mathematical models can fill in gaps where field and lab data are difficult to collect, but they need as inputs the values of certain key demographic and epidemiological ...201020436914
canine schistosomiasis in north america: an underdiagnosed disease with an expanding distribution.heterobilharzia americana, a digenean trematode in the family schistosomatidae, is the etiologic agent of canine schistosomiasis in the southeastern united states.1 a few cases of canine schistosomiasis have been reported in florida, louisiana, north carolina, texas, and, recently, kansas.1-6 the natural definitive host for the fluke is the raccoon1; however, infections have been detected in nutrias, bobcats, mountain lions, opossums, white-tailed deer, swamp rabbits, armadillos, coyotes, red wo ...201020473851
association of mc3r gene polymorphisms with body weight in the red fox and comparative gene organization in four canids.there are five genes encoding melanocortin receptors. among canids, the genes have mainly been studied in the dog (mc1r, mc2r and mc4r). the mc4r gene has also been analysed in the red fox. in this report, we present a study of chromosome localization, comparative sequence analysis and polymorphism of the mc3r gene in the dog, red fox, arctic fox and chinese raccoon dog. the gene was localized by fish to the following chromosome: 24q24-25 in the dog, 14p16 in the red fox, 18q13 in the arctic fox ...201120477806
immunohistochemical study of rabies virus within the central nervous system of domestic and wildlife species.immunohistochemistry using a commercial polyclonal antibody for lyssavirus was applied to 39 archival cases of rabies. paraffin blocks from 13 different species were available, including 3 dogs, 4 cats, 1 pig, 6 cattle, 4 horses, 1 llama, 7 skunks (mephitis mephitis), 7 raccoons (procyon lotor), 1 bat (myotis species), 1 white-tailed deer (odocoileus virginianus), 1 bobcat (lynx rufus), 2 gray foxes (urocyon cinereoargenteus), and 1 red fox (vulpes vulpes). all cases had previously been diagnose ...201020484176
longitudinal study on the seroprevalence of avian influenza, leptospirosis, and tularemia in an urban population of raccoons (procyon lotor) in ontario, canada.raccoons (procyon lotor) live at high densities, often in close association with people, in urban areas in ontario and have been implicated as potential reservoirs of numerous zoonotic disease agents. we collected 137 blood samples from 61 apparently healthy raccoons in a small area of toronto, ontario, from june to october 2007 as part of a longitudinal study to determine the seasonal patterns of seroprevalence of francisella tularensis, avian influenza, and leptospira. in addition, we collecte ...201120491583
host associations of dermacentor, amblyomma, and ixodes (acari: ixodidae) ticks in tennessee.from april 2007 to september 2008, 1,793 adult and nymphal ixodid ticks were collected from 49 counties in tennessee. six species were identified, including dermacentor variabilis (say), amblyomma americanum (l.), ixodes texanus (banks), ixodes cookei packard, ixodes scapularis (say), and amblyomma maculatum koch, from 13 medium- to large-sized mammalian hosts and dragging through vegetation. raccoons were the most common vertebrate source (198 captures), accounting for 60% of ticks collected. d ...201020496589
increased prevalence of trichinella spp., northeastern germany, 2008.in 2008, a trichinella spp. outbreak occurred on a small family-owned pig farm in mecklenburg-western pomerania in northeastern germany. to obtain epidemiologic information on this outbreak, we determined that after 2005 the prevalence of trichinella spp. in wild boars has increased in this region of germany. we discuss the potential role of the raccoon dog in the increase in trichinella spp. prevalence in the sylvatic cycle in this region. we believe that this increase could pose a threat to pi ...201020507743
genetic evidence for domestic raccoon dog rabies caused by arctic-like rabies virus in inner mongolia, china.an outbreak of rabies affected domestic raccoon dogs on an animal farm in inner mongolia, china in 2007. a study was conducted to characterize the aetiological agent and clarify the origin of the rabies virus. brain tissues were obtained from five rabid raccoon dogs. viral nucleoprotein antigen was detected in the brain tissues and five rabies viruses were isolated from these rabid animals. phylogenetic analysis of the n and g gene sequences showed that these isolates were closely related to arc ...201120546631
serological survey of five zoonoses, scrub typhus, japanese spotted fever, tularemia, lyme disease, and q fever, in feral raccoons (procyon lotor) in japan.we investigated the seroprevalence of five tick- or mite-borne zoonoses, scrub typhus (orientia tsutsugamushi), japanese spotted fever (rickettsia japonica), tularemia (francisella tularensis), lyme disease (borrelia afzelii and borrelia garinii), and q fever (coxiella burnetii), in feral raccoons (procyon lotor) captured in hokkaido and kanagawa prefectures in japan. of the 559 raccoons captured in hokkaido, 8 (1.4%), 3 (0.5%), 1 (0.2%), and 1 (0.2%) carried antibodies against o. tsutsugamushi ...201120553108
alternative transmission modes for trypanosoma cruzi.the parasite trypanosoma cruzi, which causes chagas' disease, is typically transmitted through a cycle in which vectors become infected through bloodmeals on infected hosts and then infect other hosts through defecation at the sites of subsequent feedings. the vectors native to the southeastern united states, however, are inefficient at transmitting t. cruzi in this way, which suggests that alternative transmission modes may be responsible for maintaining the established sylvatic infection cycle ...201020578791
pathways for arsenic from sediments to groundwater to streams: biogeochemical processes in the inner coastal plain, new jersey, usa.the cretaceous and tertiary sediments that underlie the inner coastal plain of new jersey contain the arsenic-rich mineral glauconite. streambed sediments in two inner coastal plain streams (crosswicks and raccoon creeks) that traverse these glauconitic deposits are enriched in arsenic (15-25mg/kg), and groundwater discharging to the streams contains elevated levels of arsenic (>80μg/l at a site on crosswicks creek) with arsenite generally the dominant species. low dissolved oxygen, low or undet ...201020580401
characterization of a panel of anti-phosphoprotein monoclonal antibodies generated against the raccoon strain of rabies virus.the generation of a new panel of 32 monoclonal antibodies (mabs) reactive with the p protein of the raccoon strain of rabies virus is described. through a series of analyses employing competitive elisa and immunoblotting, these mabs were classified into eight groups, each defining an antigenic site, thereby increasing the number of sites now recognized along the length of the p protein. studies on mab reactivity with a collection of diverse lyssaviruses identified sites that were highly conserve ...201020600390
molecular characterization of rotaviruses in a japanese raccoon dog (nyctereutes procyonoides) and a masked palm civet (paguma larvata) in japan.group a rotaviruses infect and cause diarrhea in humans and a wide range of mammals. previous studies have suggested that some strains can cross the species barrier to infect humans (martella et al., 2010). however, there are few reports on infection and characterization of rotaviruses in wild animals. to estimate what types of rotaviruses infect wild animals, we investigated infection of rotaviruses in wild animals living in urban areas in japan between 2003 and 2008. of 145 fecal specimens obt ...201020605380
heterozygote deficiencies in parasite populations: an evaluation of interrelated hypotheses in the raccoon tick, ixodes texanus.population genetics is increasingly being used to study the biology of parasites at the scales of both the host (infrapopulation, ip) and host population (component population, cp). in this study we tested three mechanistic hypotheses that could explain deviations from hardy-weinberg equilibrium (hwe) expectations due to heterozygote deficits (hds) at the cp scale in raccoon ticks (ixodes texanus; n=718) collected from raccoons (procyon lotor; n=91) and genotyped at 11 microsatellite loci. these ...201120606689
the accidental release of exotic species from breeding colonies and zoological collections.exotic species have often been introduced into a new country in zoological or botanical gardens or on game and fur farms. when accidentally or deliberately released, these alien species can become invasive and have negative impacts on native plant and animal communities and human activities. this article focuses on a selection of such invasive species: principally the american mink (neovison vison), but also the coypu (myocastor coypus), muskrat (ondatra zibethicus), raccoon dog (nyctereutes pro ...201020617652
genetic variability and viral seroconversion in an outcrossing vertebrate population.inverse correlations between genetic variability and parasitism are important concerns for conservation biologists. we examined correlations between neutral genetic variability and the presence of antibodies to canine distemper virus (cdv) and feline parvovirus (fpv) in a free-ranging population of raccoons. over 3 years there was a strong relationship between age and seroprevalence rates. most young animals were seronegative to cdv and fpv, but the oldest age class was greater than 80 per cent ...201120667873
bat rabies in massachusetts, usa, 1985-2009.to investigate rabies in massachusetts, we analyzed bat rabies test results before and after introduction of raccoon variant rabies and after release of revised 1999 us advisory committee on immunization practices recommendations for rabies postexposure prophylaxis. bat submissions were associated with level of rabies awareness and specific postexposure recommendations.201020678326
epizootiologic survey of mycobacterium bovis in wildlife and farm environments in northern michigan.bovine tuberculosis (bovine tb), caused by mycobacterium bovis, has reemerged in northern michigan, usa, with detections in white-tailed deer (odocoileus virginianus) in 1994 and in cattle in 1998. since then, significant efforts have been directed toward reducing deer densities in the area in the hopes of reducing the bovine tb prevalence rate in deer and eliminating spillover of the disease into cattle. despite the success of the efforts to reduce deer densities, additional cattle herds have b ...201020688630
asymptomatic babesia microti-like parasite infection in wild raccoon dogs (nyctereutes procyonoides) in south korea.we describe asymptomatic piroplasmosis caused by a babesia microti-like parasite in wild raccoon dogs (nyctereutes procyonoides) diagnosed by blood smear and dna analysis. this is the first description of natural b. microti-like parasite infection in raccoon dogs and suggests that the wild raccoon dog can be a source for b. microti-like parasite infection of domestic dogs and humans.201020688664
systemic staphylococcus aureus infection in a free-ranging raccoon (procyon lotor).gross and histopathologic findings are described in an adult male raccoon (procyon lotor) with staphylococcus aureus infection affecting several organs. at the time of necropsy the carcass was jaundiced and in poor nutritional state. a large, raised, irregular necrotic mass was present in the spleen. the liver had small multifocal, pale yellow, randomly distributed foci throughout the parenchyma. coagulase-positive staphylococcus aureus was cultured from both the liver and the spleen. microscopi ...201020688670
field evaluation of an inactivated vaccine to control raccoon rabies in ontario, canada.since raccoon rabies first appeared in ontario in 1999, >90,000 raccoons (procyon lotor) have received imrab3 inactivated rabies vaccine via intramuscular (im) injection and were released at the point of capture as part of a multiyear rabies control program, trap-vaccinate-release (tvr). of the 132 confirmed cases infected with raccoon-variant rabies virus in ontario between 1999 and 2005, two were from that vaccinated group, as indicated by the presence of identifying ear tags. during ongoing r ...201020688688
identification of euryhelmis costaricensis metacercariae in the skin of tohoku hynobiid salamanders (hynobius lichenatus), northeastern honshu, japan.since 2000, cutaneous nodular lesions were observed in tohoku hynobiid salamanders (hynobius lichenatus) in the northern abukuma mountains, northeastern honshu, japan. the distribution of diseased salamanders has expanded, along with the increasing severity of the disease. samples (foreleg, hindleg, and tail) from five diseased salamanders caught in the spring of 2009 contained 11 to 383 metacercarial cysts (0.43-0.48 mm in diameter) per individual. experimental inoculations of the metacercariae ...201020688689
baylisascaris procyonis in raccoons in texas and its relationship to habitat characteristics.raccoons (n=590) were collected from october 1999 to august 2003 from 35 counties across texas, usa, and gastrointestinal tracts were examined for baylisascaris procyonis. we documented b. procyonis in central and eastern texas. baylisascaris procyonis occurred in 5.4% raccoons in texas with an overall mean abundance and mean intensity of 0.4+/-0.1 and 7.4+/-2.3, respectively. prevalence was higher in central texas ecoregions and on clayey soil texture than other regions or soil textures of texa ...201020688690
optimization of raccoon latrine surveys for quantifying exposure to baylisascaris procyonis.raccoon (procyon lotor) latrine surveys are an efficient and cost-effective method to quantify baylisascaris procyonis exposure risk for intermediate hosts. nevertheless, current methodologies may introduce bias by homogenizing scats collected within latrines and estimating exposure risk at the latrine scale. the objective of this study was to examine the potential for raccoon latrine size to bias estimates of risk of exposure when utilizing current methodologies that pool scats prior to analysi ...201020688699
characterization of major histocompatibility complex (mhc) drb exon 2 and dra exon 3 fragments in a primary terrestrial rabies vector (procyon lotor).the major histocompatibility complex (mhc) presents a unique system to explore links between genetic diversity and pathogens, as diversity within mhc is maintained in part by pathogen driven selection. while the majority of wildlife mhc studies have investigated species that are of conservation concern, here we characterize mhc variation in a common and broadly distributed species, the north american raccoon (procyon lotor). raccoons host an array of broadly distributed wildlife diseases (e.g., ...201020706587
thyroid neoplasia in captive raccoons (procyon lotor).two adult, spayed, female raccoons were diagnosed with thyroid neoplasia. one raccoon had a palpable, left-sided, nonfunctional thyroid adenocarcinoma which was treated with a thyroidectomy twice with local recurrence both times. after the second recurrence, pulmonary metastases were identified. a third thyroidectomy was performed, and a vascular access port was placed for administration of intravenous doxorubicin. the raccoon developed pancytopenia and became anorexic after chemotherapy, and th ...201020722264
the complete mitochondrial genome of the raccoon dog. 201020795776
rabies surveillance in the united states during 2009.during 2009, 49 states and puerto rico reported 6,690 rabid animals and 4 human rabies cases to the cdc, representing a 2.2% decrease from the 6,841 rabid animals and 2 human cases reported in 2008. approximately 92% of reported rabid animals were wildlife. relative contributions by the major animal groups were as follows: 2,327 (34.8%) raccoons, 1,625 (24.3%) bats, 1,603 (24.0%) skunks, 504 (75%) foxes, 300 (4.5%) cats, 81 (1.2%) dogs, and 74 (1.1%) cattle. compared with 2008, numbers of rabid ...201020839985
normal aerobic bacterial conjunctival flora in the crab-eating raccoon (procyon cancrivorus) and coati (nasua nasua) housed in captivity in pernambuco and paraiba (northeast, brazil).to determine the normal aerobic bacterial conjunctival population of the coatis and the crab-eating raccoons housed in captivity in the pernambuco and paraiba states of brazil.201020840103
tick-borne disease agents in various wildlife from mississippi.because tick-borne diseases are becoming increasingly important throughout the world, monitoring their causative agents in wildlife may serve as a useful indicator of potential human exposure. we assessed the presence of known and putative zoonotic, tick-borne agents in four wildlife species in mississippi. animals were tested for exposure to or infection with ehrlichia chaffeensis, ehrlichia ewingii, borrelia lonestari, rickettsia spp., anaplasma phagocytophilum, and francisella tularensis. who ...201120846016
molecular survey of rickettsial agents in feral raccoons (procyon lotor) in hokkaido, japan.rickettsial infection in feral raccoons (procyon lotor) in hokkaido, japan was analyzed by molecular methods. genus-specific nested polymerase chain reaction (pcr) analysis based on the rickettsia citrate synthase (glta) gene showed that 13 of 699 raccoons (1.9%) examined were positive for rickettsia. twelve of the 13 partial glta sequence amplicons were successfully analyzed. the nucleotide sequence of one amplicon was identical to both rickettsia heilongjiangensis and r. japonica, one was iden ...201020859004
quantitative identification of fecal water pollution sources by taqman real-time pcr assays using bacteroidales 16s rrna genetic markers.pcr-based analysis of bacteroidales 16s rrna genes has emerged as a promising tool to identify sources of fecal water pollution. in this study, three taqman real-time pcr assays (bacgeneral, bachuman, and bacbovine) were developed and evaluated for their ability to quantitatively detect general (total), human-specific, and bovine-specific bacteroidales 16s rrna genetic markers. the detection sensitivity was determined to be 6.5 copies of 16s rrna gene for the bacgeneral and bachuman assays and 1 ...201020871990
[detection of babesia spp. dna in small mammals and ixodic ticks on the territory of north ural, west siberia and far east of russia].totally, 932 small mammals and 458 questing adult ixodes persulcatus from sverdlovsk and novosibirsk regions and khabarovsk territory, as well as 128 haemaphysalis japonica, 34 h. concinna and 29 dermacentor silvarum from khabarovsk territory were examined for the presence of babesia by nested pcr based on the 18s rrna gene. babesia microti dna was found in samples of small mammals from all the studied regions--in 36.2% of samples from sverdlovsk region, 5.3% of samples from novosibirsk region, ...201020886686
experimental infection of raccoons (procyon lotor) with west nile virus.to characterize the responses of raccoons to west nile virus (wnv) infection, we subcutaneously exposed them to wnv. moderately high viremia titers (≤ 10(4.6) plaque forming units [pfu]/ml of serum) were noted in select individuals; however, peak viremia titers were variable and viremia was detectable in some individuals as late as 10 days post-inoculation (dpi). in addition, fecal shedding was prolonged in some animals (e.g., between 6 and 13 dpi in one individual), with up to 10(5.0) pfu/fecal ...201020889868
mhc class ii drb diversity in raccoons (procyon lotor) reveals associations with raccoon rabies virus (lyssavirus).in north america, the raccoon rabies virus (rrv) is an endemic wildlife disease which causes acute encephalopathies and is a strong selective force on raccoons (procyon lotor), with estimates of ∼85% of the population succumbing to the disease when epizootic. rrv is regarded as a lethal disease if untreated; therefore, no evolutionary response would be expected of raccoon populations. however, variable immune responses to rrv have been observed in raccoons indicating a potential for evolutionary ...201020924575
new insights into the morphology, molecular characterization and identification of baylisascaris transfuga (ascaridida, ascarididae).species ranked within the genus baylisascaris (ascaridida, ascarididae) have been implicated in clinical and subclinical intestinal diseases in their natural hosts (e.g., raccoons and bears) as well as in life-threatening larva migrans syndromes in a number of incidental hosts, including humans. following the diagnosis of baylisascaris transfuga infestation in two captive polar bears, living in the zoo park of pistoia (tuscany, italy), nematodes (n=300; both sexes) have been characterized by mor ...201020947254
evaluation of genetic biodiversity in farm-bred and wild raccoon dogs in poland.the aim of the study was to analyze the intra- and inter-group diversity in farm-raised and wild raccoon dogs with the use of molecular markers. genetic differences between the particular raccoon dog groups were observed, accompanied by a relatively high intra-group genetic variation. it was noted that the wild raccoon dogs were characterized by the highest genetic diversity, compared to the three study groups of farm-bred raccoon dogs. wild raccoon dogs and farm-bred raccoon dogs constitute sep ...201020968186
[microstructure of skin derivatives as a reflection of phylogenesis of vertebrates].the possibility of using separate signs of microstructure of skin derivatives to understand phylogenesis processes at various hierarchical levels on the example of elasmoid scale of bony fish, feathers of paleognathae birds, hepatoid glands, and mammal hair was demonstrated and discussed. it was shown that (1) the presence of toothed sclerite growths on the surface of the elasmoid scale of bony fish provided with a central canal can serve as a proof of the evolutional relation of placoid and ela ...201021061666
a child with raccoon roundworm meningoencephalitis: a pathogen emerging in your own backyard?raccoon roundworm (baylisascaris procyonis) is a cause of devastating neural and ocular disease. the first documented case of raccoon roundworm encephalitis in canada, in a seven-year-old boy who presented with severe neurological impairment, is presented. his significant recovery illustrates the importance of clinical suspicion and the benefit of early treatment.200921119798
phylogenetic identification of cystoisospora spp. from dogs, cats, and raccoon dogs in japan.cystoisospora spp. from feces in dogs, cats, and raccoon dogs were isolated, sequenced at the small subunit ribosomal rna gene locus and compared to other cystoisospora spp. cystoisospora oocysts from dogs and raccoon dogs were morphologically similar with those of c. ohioensis, and cat isolates were similar with those of c. felis. the sequences from dogs and raccoon dogs, and cats have a homology with c. ohioensis and c. felis, respectively. phylogenetic analysis of the dna sequences showed tha ...201021145171
preservation of bone mass and biomechanical properties during winter sleep--the raccoon dog (nyctereutes procyonoides) as a novel model species.it has been established that almost 40% of postmenopausal women in the united states have osteopenia and models to study its prevention are thus urgently needed. bears (ursus spp.) displaying winter sleep were previously introduced as promising models to study the treatment of disuse-induced bone loss. the present study examined the potential of another analogous model species, the raccoon dog (nyctereutes procyonoides), in bone research. similar to bears, raccoon dogs display prolonged passivit ...201021146643
distribution, prevalence, and genetic characterization of baylisascaris procyonis in selected areas of georgia.baylisascaris procyonis is an intestinal nematode of raccoons (procyon lotor) that can cause fatal larval migrans in numerous species of birds and mammals, including humans. although this parasite has historically been absent in the southeastern united states, it has been found in isolated regions in the appalachian mountains and was recently documented in dekalb county, georgia. the first objective of the current study was to investigate the distribution and prevalence of b. procyonis in select ...201021158622
prins detection of 18s rdna in pig, red fox and chinese raccoon dog, and centromere dna in horse.the fluorescence in situ hybridization (fish) technique is widely used in animal cytogenetics. contrary to fish procedure, primed in situ dna synthesis (prins) does not require the dna probe preparation (design, synthesis, gel purification of pcr products and labeling). the prins method with primers used as 'dna probes' is both pcr-sensitive and allows for chromosomal localization of dna sequences. here, we show the application of prins reaction with one unlabeled oligonucleotide pair to identif ...201021166802
efficacy of the boar-operated-system to deliver baits to feral swine.feral swine (sus scrofa) pose a significant disease threat to livestock and humans. emerging technologies to reduce feral swine disease transmission risks include fertility control, vaccination, and toxicants. however, for these technologies to be appropriate for field application, a feral swine-specific oral delivery system is needed. we used two field trials to generate information related to appropriate field application of the boar-operated-system (bos™), an oral delivery system designed to ...201021176854
reducing baylisascaris procyonis roundworm larvae in raccoon latrines.baylisascaris procyonis roundworms, a parasite of raccoons, can infect humans, sometimes fatally. parasite eggs can remain viable in raccoon latrines for years. to develop a management technique for parasite eggs, we tested anthelmintic baiting. the prevalence of eggs decreased at latrines, and larval infections decreased among intermediate hosts, indicating that baiting is effective.201121192862
invasive plant species alters consumer behavior by providing refuge from predation.understanding the effects of invasive plants on native consumers is important because consumer-mediated indirect effects have the potential to alter the dynamics of coexistence in native communities. invasive plants may promote changes in consumer pressure due to changes in protective cover (i.e., the architectural complexity of the invaded habitat) and in food availability (i.e., subsidies of fruits and seeds). no experimental studies have evaluated the relative interplay of these two effects. ...201121221650
comparison of canine distemper viruses in domestic dogs and wild raccoon dogs in south korea. 201021262634
immunogenicity and efficacy of two rabies vaccines in wild-caught, captive raccoons.the immunogenicity and efficacy of two rabies vaccines in wild-caught, captive raccoons (procyon lotor) were investigated. raccoons were fed ontario slim (os) baits containing a recombinant vaccinia virus-rabies glycoprotein (vrg) oral rabies vaccine, or they were given an intramuscular (im) injection of imrab(®) 3 rabies vaccine. blood samples collected before treatment and from weeks 1 to 16 posttreatment were assessed for the presence of rabies virus antibody (rva). there were significantly m ...201121270007
intoxication of nontarget wildlife with rodenticides in northwestern kansas.the perception of prairie dogs (cynomys spp.) both as a nuisance species and a keystone species presents a significant challenge to land, livestock, and wildlife managers. anticoagulant and nonanticoagulant rodenticides are commonly employed to control prairie dog populations throughout their range. chlorophacinone, and to a lesser extent zinc phosphide, are widely used in northwestern kansas for controlling black-tailed prairie dog (cynomys ludovicianus) populations. although zinc phosphide poi ...201121270011
rabid foxes, rabid raccoons, and the odds of a human bite exposure, new york state, 1999-2007.anecdotal evidence suggests that rabid foxes are more likely to attack humans than are other rabid terrestrial animals. to examine this issue, we analyzed rabies surveillance data (1999-2007) maintained by the new york state department of health. compared to rabid raccoons (procyon lotor), foxes infected with raccoon variant rabies were more likely to bite during a human exposure incident (p<0.01). additionally, rabid gray foxes (urocyon cinereoargenteus) were significantly more likely to bite a ...201121270014
prevalence of dirofilaria immitis infection in living raccoon dogs assessed by hematological examination.the prevalence of dirofilaria immitis in free-ranging raccoon dogs (nyctereutes procyonoides viverrinus) was examined in the southeast region of kanagawa prefecture, japan, using a rapid immunomigration (rim) test kit. between april 2007 and march 2010, we examined 108 raccoon dogs rescued and housed by the kanazawa zoological garden. d. immitis infection was found in 8 (7.4%) raccoon dogs. this is the first report to reveal the prevalence of d. immitis infection in living raccoon dogs. the prev ...201121293077
combining information from surveys of several species to estimate the probability of freedom from echinococcus multilocularis in sweden, finland and mainland norway.the fox tapeworm echinococcus multilocularis has foxes and other canids as definitive host and rodents as intermediate hosts. however, most mammals can be accidental intermediate hosts and the larval stage may cause serious disease in humans. the parasite has never been detected in sweden, finland and mainland norway. all three countries require currently an anthelminthic treatment for dogs and cats prior to entry in order to prevent introduction of the parasite. documentation of freedom from e. ...201121314948
seasonality and fasting effect in raccoon dog nyctereutes procyonoides serum leptin levels determined by canine leptin-specific enzyme-linked immunosorbent assay.leptin is an adipocyte-derived peptide hormone that acts on the brain and regulates food intake and energy balance. several previous reports have suggested that overwintering raccoon dogs nyctereutes procyonoides are able to control their adiposity efficiently, but the contribution of leptin to weight regulation in these animals remains unclear. to study the seasonality of overwintering raccoon dogs as well as the effects of fasting on them, serum leptin levels were investigated using a newly es ...201021328558
differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china.in order to effectively identify the vaccine and field strains of canine distemper virus (cdv), a new differential diagnostic test has been developed based on reverse transcription-polymerase chain reaction (rt-pcr) and restriction fragment length polymorphism (rflp). we selected an 829 bp fragment of the nucleoprotein (n) gene of cdv. by rflp analysis using bamhi, field isolates were distinguishable from the vaccine strains. two fragments were obtained from the vaccine strains by rt-pcr-rflp an ...201121352564
molecular characterization of korean rabies virus isolates.the nucleoprotein (n) and glycoprotein (g) of 11 korean rabies virus (rabv) isolates collected from animals diagnosed with rabies between 2008 and 2009 were subjected to molecular and phylogenetic analyses. six isolates originated from domestic animals (cattle and dogs) and five were obtained from wild free-ranging raccoon dogs. the similarities in the nucleotide sequences of the n gene among all korean isolates ranged from 98.1 to 99.8%, while those of the g gene ranged from 97.9 to 99.3%. base ...201121368564
effects of alprazolam on capture stress-related serum cortisol responses in korean raccoon dogs (nyctereutes procyonoides koreensis).the purpose of this study was to evaluate the effect of alprazolam on the stress that korean raccoon dogs (nyctereutes procyonoides koreensis) may experience while caught in a live trap by measuring their serum cortisol response. the animals were placed in a live trap with or without being pretreated with oral doses of alprazolam. in both groups, pre-trap blood samples were initially collected without anesthesia before the animals were positioned in the live trap; then post-trap blood samples we ...201121368571
the first detection of babesia species dna from japanese black bears (ursus thibetanus japonicus) in japan.in this study, we tried to detect protozoan blood parasites from the liver or blood of 156 japanese black bears (ursus thibetanus japonicus) in iwate prefecture of japan by polymerase chain reaction. two amplicons (approximately 540 bp and 480 bp) were detected by amplification for v4 hyper-variable regions of the 18s rrna gene. approximately 540-bp products were obtained in 119 samples (76.3%) and were considered to be dna of hepatozoon ursi. approximately 480-bp products were obtained in 22 sa ...201121376138
molecular survey of anaplasma and ehrlichia infections of feral raccoons (procyon lotor) in hokkaido, japan.infection by anaplasma and ehrlichia in feral raccoons (procyon lotor) in hokkaido, japan, was examined by molecular methods. a polymerase chain reaction (pcr) screen for anaplasmataceae, based on 16s rrna, showed that 38 (5.4%) of 699 raccoons examined were positive. these 38 positive samples were examined for anaplasma phagocytophilum, anaplasma bovis, ehrlichia chaffeensis, and ehrlichia canis infection by species-specific nested pcr. nested pcr results indicated that 36 of the 38 samples wer ...201121395415
raccoon roundworms in pet kinkajous--three states, 1999 and 2010.baylisascaris procyonis (bp) is the common roundworm of raccoons (procyon lotor). adult bp live in the small intestine of this host, where they produce eggs that are passed in the feces. bp eggs ingested by nondefinitive host species hatch in the intestine, producing larvae that can migrate widely, causing visceral, ocular, or neural larva migrans. cases of neural larva migrans in humans caused by bp likely acquired from raccoons have resulted in severe encephalitis with permanent deficits and i ...201121412211
salmonella in raccoons (procyon lotor) in southern ontario, canada.numerous serotypes of salmonella have been detected in a variety of wild animals, including raccoons (procyon lotor). raccoons are common, mid-size omnivores that live in close association with people in urban and rural areas in ontario. although raccoons are known to shed salmonella, little is known about their potential long-term role in maintaining salmonella infections. we sampled feces from raccoons in three areas of ontario: one primarily urban site around niagara, one primarily rural site ...201121441187
a unique genotype of leptospira interrogans serovar pomona type kennewicki is associated with equine abortion.although serologic data indicate horses in n. america are exposed to a variety of leptospiral serovars, abortion is almost always associated with leptospira interrogans serovar pomona type kennewicki. a variety of wildlife including raccoons, white tailed deer, striped skunks, opossums, and red and grey foxes have been shown to host serovar pomona and have therefore been suspect as sources of infection for pregnant mares. the aim of the present study was to examine genetic diversity in serovar p ...201121450416
experimental inoculation of raccoons (procyon lotor) with spiroplasma mirum and transmissible mink encephalopathy (tme).the primary objective of this study was to determine whether or not spiroplasma mirum would be capable of producing lesions of transmissible spongiform encephalopathy (tse) when inoculated in raccoons (procyon lotor) and, if that was possible, to compare the clinicopathological findings with those of transmissible mink encephalopathy (tme) in the same experimental model. for this purpose, 5 groups (n = 5) of raccoon kits were inoculated intracerebrally with either s. mirum and/or tme. two other ...201121461191
activity pattern, reproductive behaviors and gonadal hormones in the raccoon dog (nyctereutes procyonoides).the objectives of this study were to (1) assess year-round behaviors and activity patterns of captive raccoon dogs (nyctereutes procyonoides) and (2) characterize the species' reproductive endocrinology. behaviors and activity patterns of 12 (5.7) animals were recorded over a 1-year period. during that time, fecal samples were collected 2-7 times/week from 16 (7.9) individuals (six of these were included in the behavioral study) for the analysis of testosterone, progesterone and estrogen metabol ...201121462244
molecular epidemiology of rabies virus circulating in south korea, 1998-2010.we analyzed the nucleotide sequences of the g-l (glycoprotein-large protein) intergenic non-coding region of 33 strains of the rabies virus (rabv) isolated in south korea in 1998-2010 and compared the sequences with those of previously reported non-korean strains. the similarities of the nucleotide sequences of the g-l region among all korean rabv isolates ranged from 97.1 to 100%. based on the phylogenetic analysis of the g-l region, the korean rabv isolates were classified into three distinct ...201121467756
highly pathogenic avian influenza virus infection in feral raccoons, japan.although raccoons (procyon lotor) are susceptible to influenza viruses, highly pathogenic avian influenza virus (h5n1) infection in these animals has not been reported. we performed a serosurvey of apparently healthy feral raccoons in japan and found specific antibodies to subtype h5n1 viruses. feral raccoons may pose a risk to farms and public health.201121470469
[introduction of species and microevolution: the european beaver, raccoon dog, and american mink].nine skull samples of the beaver castor fiber, six samples of the raccoon dog nyctereutes procyonoides, and six samples of the american mink neovison vison were studied using phenetic and craniometric methods. analysis of the phenofund structure suggests that in all of the studied species the emergence of novel character variations does not lead to their fixation with a significant frequency. considerable morphological variability emerges in the contact zone of different autochtonous populations ...201121506393
novel hepatozoon in vertebrates from the southern united states.abstract novel hepatozoon spp. sequences collected from previously unrecognized vertebrate hosts in north america were compared with documented hepatozoon 18s rrna sequences in an effort to examine phylogenetic relationships between the different hepatozoon organisms found cycling in nature. an approximately 500 base pair fragment of 18s rdna common to hepatozoon spp. and some other apicomplexans was amplified and sequenced from the tissues or blood of 16 vertebrate host species from the souther ...201121506825
helminth parasites of an introduced invasive carnivore species, the raccoon ( procyon lotor l.), from the warta mouth national park (poland).abstract we evaluated helminth parasites of the introduced north american raccoon ( procyon lotor l.) in poland. from june 2006 to may 2007, 91 raccoon fecal samples were collected in the warta mouth national park situated in western poland near the polish-german border. coprological analyses involved flotation and sedimentation. in total, 7 helminth taxa were identified in the feces: ancylostoma spp., baylisascaris procyonis , capillariidae, placoconus lotoris , spirocerca lupi , strongyloides ...201121506875
seroprevalence of toxoplasma gondii infection in feral raccoons (procyon lotor) in japan.abstract antibodies to toxoplasma gondii were found in 92 (9.9%) of 929 feral raccoons (procyon lotor) in japan using the latex agglutination test (lat, 1: 64 or higher). seropositivity varied by geographic location, season, weight, but by the sex of raccoons trapped. seroprevalences in the northern, central, and western areas of japan were found to be 7.9% (39/492), 16.5% (47/285), and 3.9% (6/152), respectively. the seroprevalence by season varied from 8.5% (13/153) in spring to 18.9% (14/74) ...201121510744
re-assessment of direct fluorescent antibody negative brain tissues with a real-time pcr assay to detect the presence of raccoon rabies virus rna.the first report of the raccoon variant of rabies virus was in ontario, canada in 1999. as part of the control of this outbreak a point infection control (pic) strategy of trapping and euthanizing vector species was implemented. to evaluate whether this strategy was indeed removing diseased animals, rabies diagnosis was performed on these specimens. during a pic program conducted in 2003, 721 animals (raccoons, striped skunks and red foxes) were collected and euthanized and brain material from e ...201121514325
development and characterization of 12 polymorphic microsatellite loci in the american dog tick (dermacentor variabilis).twelve polymorphic microsatellite loci were developed for the american dog tick (dermacentor variabilis), an important vector of infectious diseases in humans and animals. four multiplexed panels comprising the loci were developed and 45 ticks collected from two raccoons (procyon lotor) were genotyped. the number of alleles per locus ranged from nine to 30, and single locus heterozygosities ranged from 0.18 to 0.93. data generated using these markers will further our understanding of factors aff ...200921564581
development and characterization of 14 polymorphic microsatellite loci in the raccoon tick (ixodes texanus).fourteen polymorphic microsatellite loci were developed for the raccoon tick (ixodes texanus). three multiplexed panels comprising the loci were developed and 50 ticks collected from two infected raccoons (procyon lotor) were genotyped. the number of alleles per locus ranged from five to 22, and single locus heterozygosities ranged from 0.46 to 0.94. future research will further our understanding of i. texanus biology and help in elucidating the effects of life-history variation on parasite popu ...200921564631
species identification of north american guinea worms (nematoda: dracunculus) with dna barcoding.dracunculus insignis is a nematode parasite that infects the subcutaneous tissues of mammals such as raccoon (procyon lotor), mink (neovison vison) and fisher (martes pennanti). d. lutrae, a morphologically similar species, has only been recovered from the otter (lontra canadensis). species identification of these two north american guinea worms has only been achieved by morphology of males and host identity. as a result, where only female specimens are present, accurate identifications are not ...200921564728
mathematical models for rabies.rabies virus and its associated host-pathogen population dynamics have proven a remarkable model system for developing mathematical models of infectious disease emergence and spread. beginning with simple susceptible-infectious-removed (sir) compartment models of fox rabies emergence and spread across western europe, mathematical models have now been developed to incorporate dynamics across heterogeneous landscapes, host demographic variation, and environmental stochasticity. model structures ra ...201121601056
evolution of wildlife rabies control tactics.the development of tactics for the control of rabies in wildlife species has evolved dramatically during the past few decades in part due to research advances. historically, rabies control measures primarily involved the culling of target species. however, contemporary advances in the research and development of oral rabies vaccines and delivery systems for wildlife have now made it feasible to treat rabies outbreaks over thousands of square kilometers of habitat. systems have been developed to ...201121601057
understanding effects of barriers on the spread and control of rabies.this chapter reviews the evidence for the impact of natural and anthropogenic barriers on the spread of rabies using evidence mainly drawn from the epidemics of fox and raccoon variant rabies virus over the past 60 years in north america. those barriers have both directed and inhibited the spread of rabies and, at a regional scale, have been integrated with rabies control efforts in north america. few studies have been done, however, to examine how the texture (grain) and configuration of the ha ...201121601058
genetic tracking of the raccoon variant of rabies virus in eastern north america.to gain insight into the incursion of the raccoon variant of rabies into the raccoon population in three canadian provinces, a collection of 192 isolates of the raccoon rabies virus (rrv) strain was acquired from across its north american range and was genetically characterized. a 516-nucleotide segment of the non-coding region between the g and l protein open reading frames, corresponding to the most variable region of the rabies virus genome, was sequenced. this analysis identified 119 differe ...201121624778
spinal cord involvement in a child with raccoon roundworm (baylisascaris procyonis) meningoencephalitis.a 14-month-old previously healthy boy developed progressively worsening neurological symptoms secondary to eosinophilic meningoencephalitis with myelitis caused by raccoon roundworm (baylisascaris procyonis) infection. mri demonstrated t2 hyperintensity and enhancement of the cerebral white matter, cerebellum and spinal cord. prior case reports have described signal abnormality within the brains of patients with raccoon roundworm neural larva migrans (nlm). this is a unique case in which spinal ...201121629989
host and viral ecology determine bat rabies seasonality and maintenance.rabies is an acute viral infection that is typically fatal. most rabies modeling has focused on disease dynamics and control within terrestrial mammals (e.g., raccoons and foxes). as such, rabies in bats has been largely neglected until recently. because bats have been implicated as natural reservoirs for several emerging zoonotic viruses, including sars-like corona viruses, henipaviruses, and lyssaviruses, understanding how pathogens are maintained within a population becomes vital. unfortunate ...201121646516
advancements in web-database applications for rabies surveillance.abstract: background: protection of public health from rabies is informed by the analysis of surveillance data from human and animal populations. in canada, public health, agricultural and wildlife agencies at the provincial and federal level are responsible for rabies disease control, and this has led to multiple agency-specific data repositories. aggregation of agency-specific data into one database application would enable more comprehensive data analyses and effective communication among par ...201121810215
hematology and serum biochemistry in debilitated, free-ranging raccoon dogs (nyctereutes procyonoides) infested with sarcoptic mange.frequent outbreaks of sarcoptes scabiei infestation in raccoon dogs (nyctereutes procyonoides) have been reported in japan. although many raccoon dogs are brought to kanazawa zoological garden (yokohama, kanagawa, japan) because of s. scabiei infestation and debilitation, some of them die of asthenia. the clinical status of severely debilitated raccoon dogs must be determined to save their lives. in this study, we compared hematological and serum biochemical values between severely debilitated a ...201121749931
leptospirosis and tularaemia in raccoons (procyon lotor) of larimer country, colorado.raccoons (procyon lotor) are commonly implicated as carriers of many zoonotic pathogens. the purpose of this cross-sectional study was to look for leptospira interrogans and francisella tularensis in opportunistically sampled, free-ranging raccoons of larimer country, colorado, usa. sixty-five animals were included in the study and testing consisted of gross post-mortem examination, histopathology, and both immunohistochemistry and pcr for l. interrogans and f. tularensis. no significant gross l ...201121824365
a recombinant antigen-based enzyme-linked immunosorbent assay for specific diagnosis of baylisascaris procyonis larva migrans.baylisascaris larva migrans is an important zoonotic disease caused by baylisascaris procyonis, the raccoon roundworm, and is being increasingly considered in the differential diagnosis of eosinophilic meningoencephalitis in children and young adults. although b. procyonis excretory-secretory (bpes) antigen-based elisa and western blot assays are useful in the immunodiagnosis of this infection, cross-reactivity remains a major problem. recently, a recombinant b. procyonis antigen, bprag1, was re ...201121832102
deja vu: the evolution of feeding morphologies in the carnivora.the fossil record of the order carnivora extends back at least 60 million years and documents a remarkable history of adaptive radiation characterized by the repeated, independent evolution of similar feeding morphologies in distinct clades. within the order, convergence is apparent in the iterative appearance of a variety of ecomorphs, including cat-like, hyena-like, and wolf-like hypercarnivores, as well as a variety of less carnivorous forms, such as foxes, raccoons, and ursids. the iteration ...200721672827
[phylogenetic analysis of rabies viruses isolated from animals in tokyo in the 1950s].molecular epidemiological analysis of 96 rabies viruses isolated from animals in tokyo in the 1950s involves japanese fixed virus, komatsugawa, takamen, and nishigahara strains. strains isolated in tokyo were divided into tokyo 1 and tokyo 2, and grouped into a worldwide distribution cluster differing from takamen and nishigahara. tokyo 1 was grouped into the same cluster as viruses isolated from united states west coast dogs in the 1930s and 1940s. tokyo 2 was grouped into the same cluster as t ...201121706842
epizootic canine distemper virus infection among wild mammals.in the spring of 2007, seven raccoon dogs and a weasel were captured near the city of tanabe in wakayama prefecture, japan. the causative agent of the animals' death 1-2days after capture was identified as canine distemper virus (cdv) by virus isolation, immunostaining with an anti-cdv polyclonal antibody, and a commercially available cdv antigen-detection kit. sequence analysis of hemagglutinin genes indicated the isolated viruses belong to genotype asia-1 and possess the substitution from tyro ...201121840141
comparison of the host specificities of two bacteroidales quantitative pcr assays used for tracking human fecal contamination.the sewage-associated real-time quantitative pcr (qpcr) assays bachum and hf183 sybr were compared for specificity against local fecal sources. both assays were equally sensitive to sewage, but bachum showed substantially more false-positive results for cat, dog, gull, and raccoon feces.201121742921
Prevalence of Salmonella, Yersinia and Campylobacter spp. in Feral Raccoons (Procyon lotor) and Masked Palm Civets (Paguma larvata) in Japan.To estimate the public and animal health risk that alien species pose, the prevalence of Salmonella, Yersinia, and Campylobacter spp. in feral raccoons (Procyon lotor, nÔÇâ=ÔÇâ459) and masked palm civets (Paguma larvata, nÔÇâ=ÔÇâ153), which are abundant alien species in Japan, was investigated in urban and suburban areas of Japan. Salmonella enterica was detected from 29 samples [26 raccoons, 5.7%, 95% confidence interval (CI) 7.8-3.5%; three masked palm civets, 2.0%, 95% CI 4.2-0%]. Many of the ...201121824337
clinical and pathologic characteristics of t-cell lymphoma with a leukemic phase in a raccoon dog (nyctereutes procyonoides).a 7.5-year-old raccoon dog (nyctereutes procyonoides) from the henry doorly zoo (omaha, nebraska) presented to the veterinary hospital for lethargy and weight loss. on physical examination, splenomegaly and hepatomegaly were noted on palpation and were confirmed by radiographic evaluation. radiography also demonstrated a mass in the cranial mediastinum. a complete blood cell count revealed marked leukocytosis (115,200 cells/µl), with a predominance of lymphoid cells. the animal was euthanized du ...201121908331
Zoonoses of procyonids and nondomestic felids.There are several important zoonotic diseases which can be acquired from procyonids, and nondomestic felids. Baylisascaris procyonis, the raccoon roundworm, is a common parasite of raccoons and can cause visceral, ocular, or neural larval migrans in people. Neural larval migrans can cause severe signs in individuals. Dermatophytosis and enteric pathogens are the most important zoonotic agents found in nondomestic felids. Microsporum canis infections can be spread from nondomestic felids to owner ...201121872788
rabies surveillance in the united states during 2010.during 2010, 48 states and puerto rico reported 6,154 rabid animals and 2 human rabies cases to the cdc, representing an 8% decrease from the 6,690 rabid animals and 4 human cases reported in 2009. hawaii and mississippi did not report any laboratory-confirmed rabid animals during 2010. approximately 92% of reported rabid animals were wildlife. relative contributions by the major animal groups were as follows: 2,246 raccoons (36.5%), 1,448 skunks (23.5%), 1,430 bats (23.2%), 429 foxes (6.9%), 30 ...201121916759
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