| expression of epstein-barr virus genes in different cell types after microinjection of viral dna. | gene expression of epstein-barr virus (ebv) was studied after microinjection of viral dna into different types of cells. raji tk- cells, known to express viral gene functions after superinfection with the ebv-p3hr-1 virus strain, were attached to plastic dishes by using anti-lymphocyte igg, phytohemagglutinin, or concanavalin a as a ligand. it was difficult to inject dna into the small and fragile raji cells. after formation of polykaryons by cell fusion, microinjection became more efficient. at ... | 1980 | 6244558 |
| dna sequence of baboon highly repeated dna: evidence for evolution by nonrandom unequal crossovers. | a highly repeated dna was isolated from the west african baboon (papio papio) as a 343-base-pair fragment after digestion of total baboon dna with the restriction endonuclease bamhi. the dna sequence of this fragment was obtained by chemical cleavage methods and is compared with the dna sequence of related highly repeated primate dnas from african green monkey (cercopithecus aethiops) and man. the 343-base-pair baboon repeat consists of two related but nonidentical wings of 172 and 171 base pair ... | 1980 | 6246524 |
| virus-specific basic phosphoproteins associated with herpes simplex virus type a (hsv-1) particles and the chromatin of hsv-1-infected cells. | herpes simplex type 1 angelotti (hsv-1 ang) virions were shown to contain two major acid-soluble proteins, bp1 and 2, which by size and charge analysis were also found to be associated with chromatin isolated from hsv-1 ang-infected african green monkey kidney cells (hsv-chromatin). bp1 and 2 proved to exist in a phosphorylated state both in virions and in hsv-chromatin. bp1 consisted of a single polypeptide of 38 k mol. wt. which was correlated to the tegument protein vp22. in sds-polyacrylamid ... | 1980 | 6247428 |
| carcinogenicity and hepatotoxicity of cycasin and its aglycone methylazoxymethanol acetate in nonhuman primates. | the carcinogenic potential of cycasin and methylazoxymethanol (mam) acetate was investigated in nonhuman primates. old-world monkeys (rhesus, cynomolgus, and african green monkeys) received cycasin and/or mam acetate by oral or ip routes up to 11 years. eighteen monkeys survived longer than 2 months after initiation of treatment with cycasin (50-75 mg/kg) or mam acetate (1.5-3.0 mg/kg) given orally 5 days/week; 9 of the animals were necropsied. histopathologic examination of a liver tumor from 1 ... | 1980 | 6248673 |
| chromatin-associated protein kinases specific for acidic proteins. | protein kinases (ec 2.7.1.37) were eluted by 0.4 nacl from chromatin of several mammalian cell typs. the enzymes were partially purified by ion-exchange chromatography, dna-cellulose columns and sucrose gradient centrifugation. at least five different enzymes could be distinguished by their biochemical properties and their substrate specificities. three of the enzyme activities tested phosphorylate different sets of histones, while two enzymes phosphorylate acidic nonhistone chromatin proteins o ... | 1980 | 6249376 |
| influence of trypsin on the infectivity and biological properties or parainfluenza type 2 type 2 (croup-associated) virus in vero cells. | a comparative study on the infectivity and biological activities of parainfluenza virus type 2 revealed that one strain (toshiba) showed c.p.e. with cell fusion and produced plaques in vero cells, an established line of african green monkey kidney cells. another strain (62-m786) of this virus, however, showed minimum c.p.e. and produced almost no plaques in vero cells, although c.p.e. appeared and plaques were observed following addition of trypsin. the infectivity titre of the latter strain, es ... | 1980 | 6249888 |
| preferential replication of a class of host-substituted defective simian virus 40 variants at low temperature. | the host-substituted variant termed cvp8/1/p2 (ecori res) was first isolated several years ago after serial passage of simian virus 40 strain 777 on bsc-1 cells at 37 degrees c. when bsc-1 are coinfected with wild-type simian virus 40 strain 777 and variant cvp8/1/p2 (ecori res), the variant rapidly becomes the dominant species produced, often representing as much as 80% of the total dna i synthesized after infection. we present evidence that the replicative advantage of the variant was increase ... | 1980 | 6251262 |
| indiscriminate recombination in simian virus 40-infected monkey cells. | dna transfection of african green monkey bsc-1 cells with simian virus 40 (sv40) dna and bacterial virus phi x174 replicative form dna ("cotransfection") yielded stocks containing sv40/phi x174 recombinant virus, which was detected by an infectious-center in situ plaque hybridization procedure and which was sensitive to anti-sv40 antiserum. the recombinant virus replicated during serial passage. restriction endonuclease cleavage of the sv40/phi x174 dna indicated that several different types of ... | 1980 | 6254043 |
| control of sv40 replication by a simple chromosome in monkey-hamster cell hybrids. | somatic cell hybrids produced between cercopithecoid monkey and chinese hamster cells were used to assay susceptibility to sv40 viral infection in an attempt to define the primate factors that determine permissiveness to viral replication. these cell hybrids, which differed in their primate chromosome complement, were found to differ also in their ability to sustain viral replication. a correlation was found between an elevated sv40 viral replication and the presence of the chromosomes 11 in the ... | 1980 | 6255616 |
| antiviral activity of antilipidemic compounds on herpes simplex virus type 1. | two antilipidemic compounds, clofibrate and procetofene, inhibited the replication of herpes simplex virus type 1 (hsv-1) in african green monkey kidney cells. clofibrate, at a concentration of 400 mumol/liter caused a 63% reduction (p < 0.001) in hsv-1 yield and at 100 mumol/liter caused a 62% reduction (p < 0.001) in plaque formation. two stereoisomeric analogs of clofibric acid, (-)- and (+)-desmethyl clofibric acid, also caused a significant inhibition of hsv-1 replication. procetofene at 5 ... | 1980 | 6255860 |
| [studies on the activity of the aryl hydrocarbon hydroxylase and metabolism of benzo(a)pyrene in cell strains and cell lines used for testing cytotoxic and transforming ability of foreign compounds (author's transl)]. | the activity of the ahh and the formation of polar metabolites from bap was examined in several cell strains and lines, which are used for testing cytotoxic and transforming activity of environmental agents, the highest activity of the ahh and the highest capacity to metabolize bap was found in hamster kidney cells. much lower values were measured in kidney cells from cercopithecus aethiops, bhk21/c13 cells, and sv-40 transformed hamster kidney cells (14-1b). the activity of ahh was inducible by ... | 1980 | 6256996 |
| a histone-specific acetyltransferase is associated with simian-virus-40 chromatin. | [14c]thymidine-labeled simian virus 40 nucleoprotein complexes were prepared from nuclei of lytically infected african green monkey kidney cells. the nucleoprotein complexes were further purified by sucrose gradient centrifugation and then incubated in the presence of [3h]acetyl-coenzyme a. we observed a transfer of [3h]acetyl groups to the endogenous histones h2b, h3 and h4. the enzyme was solubilized in the presence of 0.5 m nacl and showed properties described for the dna-binding acetyltransf ... | 1980 | 6257509 |
| sequence relationships between single repeat units of highly reiterated african green monkey dna. | individual monomer and dimer units of the highly repeated alpha-component dna of african green monkeys were isolated and amplified by molecular cloning in pbr322. the purified sequences were characterized by digestion with restriction endonucleases and by primary nucleotide sequence analysis. comparison of the cloned units with the 172 base pair long sequence representing the most abundant nucleotide at each position in the set of sequences comprising alpha-component allows the following conclus ... | 1981 | 6259618 |
| protein phosphorylations in poliovirus infected cells. | in vivo phosphorylation of proteins that are associated with polysomes of poliovirus-infected vero (african green monkey kidney) and hela (henrietta lacks) cells differed from phosphorylations observed with uninfected cells that were fed fresh medium. with both types of cells infection stimulated phosphorylation of proteins with molecular weights of 40 000-41 000, 39 000, 34 000, 32 000, and 24 000. similarities of phosphorylations in vero and hela cells suggest that they are a specific conseque ... | 1981 | 6260321 |
| propagation of human hepatitis a virus in african green monkey kidney cell culture: primary isolation and serial passage. | human hepatitis a virus (hav) was propagated in primary african green monkey (cercopithecus aethiops) kidney (agmk) cell cultures. three strains of hav were used: ms-1, sd-11, and hm-175. cells were inoculated with marmoset-passaged material or human clinical specimens and were stained by direct immunofluorescence to establish the identity of the virus. both clinical samples and marmoset-passaged material produced immunofluorescence. hav antigen was found scattered throughout the cytoplasm of in ... | 1981 | 6260685 |
| recurring defective variants of simian virus 40 containing monkey dna segments. | four independently and newly isolated defective variants of simian virus 40 have been characterized. all four are very similar, if not identical, to two previously and independently isolated variants (wakamiya et al., j. biol. chem. 254:3584-3591, 1979; j. papamatheakis, e. kuff, e. winocour, and m. f. singer, j. biol. chem. 255:8919-8927, 1980). the documented similarities include restriction endonuclease maps and the presence of the same monkey dna segments covalently linked to simian virus 40 ... | 1981 | 6260983 |
| the dna sequences of cloned complex satellite dnas from hawaiian drosophila and their bearing on satellite dna sequence conservation. | a class of restriction endonuclease fragments near 185 bp in length and comprising approximately 20% of the genomes of 3 species of hawaiian drosophila has been cloned using bacteriophage m13. the nucleotide sequences of 14 clones have been determined and the variation between clones has been found to be due to deletions and base changes. analyses of uncloned material show that the cloning system itself does not introduce the variation. the variation of the basic repeat within and between specie ... | 1981 | 6262029 |
| plaque formation by human-origin parainfluenza type 2 virus in established cell lines. | two strains of parainfluenza type 2 virus formed well-defined plaques in cultures of vero cells, an established line of african green monkey kidney cells. in the absence of trypsin, satisfactory plaques were formed by the toshiba strain of virus. when trypsin was added in the overlay medium of vero cell monolayers, another strain (62-m786) of virus produced plaques. the toshiba strain was also able to make plaques in hela, bhk, and llc-mk2 (an established line of monkey kidney) cells without try ... | 1981 | 6263231 |
| highly sensitive assay for clostridium perfringens enterotoxin that uses inhibition of plating efficiency of vero cells grown in culture. | a highly sensitive and reproducible biological assay for clostridium perfringens enterotoxin is described that uses vero (african green monkey kidney) cells grown in tissue cultures. very small doses of the enterotoxin inhibited the plating efficiency of the cells. this inhibition of plating efficiency could be used to detect as little as 0.1 ng (1 ng/ml) of enterotoxin, and a linear dose-response curve was obtained with 0.5 to 5 ng (5 to 50 ng/ml). a nonlinear, but reproducible, curve was obtai ... | 1981 | 6263946 |
| dna sequences similar to those around the simian virus 40 origin of replication are present in the monkey genome. | we report the molecular cloning of african green monkey genomic dna segments that include regions of homology to the origin of replication of simian virus 40 (sv40). three clearly different cloned segments 14 to 17 kilobase pairs (kb) long were isolated from a genomic library in lambda phage. we estimate that each of the three is repeated fewer than four times in the monkey genome. the sv40-like regions represent a small portion of the cloned segments, and these regions cross hybridize only weak ... | 1981 | 6264457 |
| molecular biology of rotaviruses. i. characterization of basic growth parameters and pattern of macromolecular synthesis. | the united kingdom tissue-adapted bovine rotavirus growing in african green monkey kidney (bsc-1) cells was selected as a model system with which to study the detailed molecular virology of rotavirus replication. study of the kinetics of infectious virus production revealed a fairly rapid replication cycle, with maximum yield of virus after 10 to 12 h at 37 degrees c. progeny genome synthesis was first detected during the virus latent period at 2 to 3 h postinfection. study of the kinetics of vi ... | 1981 | 6268838 |
| in situ electron microscopical observation of cells infected with herpes simplex virus. | transport and release of herpes simplex virus (hsv) in an african green monkey kidney cell line (cv-1) was followed by electron microscopy for up to 24 h p.i. transmission electron microscopy and scanning electron microscopy were employed. for the former approach, electron microscopical autoradiography of whole cultured cells and in situ thin section techniques were used. the following new observations were made. (1) except in peripheral parts of the cells, where the cytoplasmic membrane could m ... | 1981 | 6270236 |
| detection and cloning of murine leukemia virus-related sequences from african green monkey liver dna. | by using low-stringency nucleic acid hybridization conditions and specific subgenomic segments of the akr ecotropic provirus as probes, murine leukemia virus (mulv)-related sequences were detected in african green monkey (agm) liver dna. the mulv-reactive segments present in restricted agm dna ranged from 1.9 kilobases (kb) to greater than 10 kb in size. on the basis of this finding, a 17-kb segment was cloned from a partial ecori agm library in lambda charon 4a which shared nearly 5 kb of homol ... | 1981 | 6270353 |
| biological properties and carbohydrate composition of human parainfluenza virus type 1 in two host systems. | human parainfluenza virus type 1 (ha2 virus) grown either in embryonated hens' eggs or in m. rhesus monkey kidney cells showed differences in size, biological properties and carbohydrate composition. egg-grown virus showed a larger size (233 nm versus 167 nm), a higher neuraminidase activity (specific activity and initial and maximum velocity) and a higher haemolytic activity than monkey kidney cell-grown virus. the haemagglutinin titre was identical for the ha2 strain grown in both host systems ... | 1981 | 6271905 |
| identification and cloning of endogenous retroviral sequences present in human dna. | using nonstringent annealing conditions and a 2.75-kilobase segment of cloned african green monkey dna that specifically hybridizes to the proviruses of akr ecotropic murine leukemia virus (mulv) and baboon endogenous virus (baev) as a probe, we detected related sequences in three different preparations of human brain dna fragments. the blot-hybridization pattern obtained with cleaved human dna was similar to that previously reported for the interaction of mulv cdna and cleaved mouse dna and sug ... | 1981 | 6272283 |
| interspersed repeated sequences in the african green monkey genome that are homologous to the human alu family. | the dominant family of interspersed repetitive dna sequences in the human genome has been termed the alu family. we have found that more than 75% of the lambda phage in a recombinant library representing an african green monkey genome hybridize with a human alu sequence under stringent conditions. a group of clones selected from the monkey library with probes other than the alu sequence were analyzed for the presence and distribution of alu family sequences. the analyses confirm the abundance of ... | 1981 | 6273798 |
| liposome-mediated delivery of deoxyribonucleic acid to cells: enhanced efficiency of delivery related to lipid composition and incubation conditions. | delivery of liposome-encapsulated simian virus 40 (sv40) dna to african green monkey related to been used as a probe to study liposome--cell interactions and to determine conditions which favor the intracellular delivery of liposome contents to cells. the efficiency of dna delivery by various liposome preparations (monitored by infectivity assays) was found to be dependent both on the magnitude of vesicle binding to cells and on the resistance of liposomes to cell-induced leakage of contents. ac ... | 1981 | 6274382 |
| rickettsia sennetsu in human blood monocyte cultures: similarities to the growth cycle of ehrlichia canis. | microscopic examination of cultured human monocytes infected with rickettsia sennetsu and stained by the giemsa method revealed the presence of various organismal growth forms in the cytoplasm of the infected cells. the growth forms were loosely scattered individual organisms, clusters of organisms, various sizes of dense inclusion bodies in intact and vacuolated cytoplasm, and organisms in close proximity to disintegrated monocytes. the appearance and the morphology of these r. sennetsu growth ... | 1982 | 6274798 |
| nucleosome phasing and micrococcal nuclease cleavage of african green monkey component alpha dna. | the micrococcal nuclease cleavage of intact nuclear chromatin from african green monkey cells and of the completely deproteinized sequences was studied by using high-resolution analytical and dna sequencing gels and secondary restriction enzyme analysis. when deproteinized component alpha dna was used as substrate, not all phosphodiester bonds in the 172-base-pair repeat units were cleaved with equal frequency by the nuclease. a distinct preference for the cleavage of a-t rather than g-c bonds w ... | 1982 | 6275381 |
| a monkey alu sequence is flanked by 13-base pair direct repeats by an interrupted alpha-satellite dna sequence. | a member of the alu family, the dominant family of short interspersed repeated dna sequences in primates, interrupts a cloned repeat unit of african green monkey alpha-satellite dna. the alu is immediately flanked by 13-base-pair duplications of the known sequence of the satellite at the site of insertion. these observations support the idea that ala family members may be moveable elements. | 1982 | 6280186 |
| expression of mouse immunoglobulin genes in monkey cells. | the generation of functional immunoglobulin light-chain genes from variable (v), joining (j), and constant (c) gene segments has been elucidated recently. the structural features which regulate the expression of immunoglobulin genes, however, have not been well defined. to further our understanding of immunoglobulin gene expression, african green monkey cells (cv1), hela cells, and mouse l cells were transfected with kappa-chain gene-containing plasmids. we report here that efficient transient e ... | 1982 | 6283383 |
| [experiment in infecting the green monkey cercopithecus aethiops with a leukocyte virus isolated in hepatitis a]. | the virus isolated in hepatitis a had been previously shown to passage in primary phytohemagglutinin-stimulated leukocyte cultures. the virus designated as leukocyte hepatitis virus (lhv) causes chromosome aberrations in the infected cultures. some physico-chemical properties of lhv and its capacity for reproduction in other cell systems were studied. in serological surveys of patients and in studies of cellular immunity reactions of them lhv was found in cases of hepatitis. the possibility of ... | 1982 | 6283739 |
| [use of a cocultivation method for detecting cytomegalovirus contamination of cell cultures of simian origin]. | at present, nonanthropoid primates are widely used as sources of cell cultures for manufacture of live viral vaccines. simian cell cultures, particularly kidney cell cultures are also known to be frequently contaminated with cytomegaloviruses. the isolation of the latter is rather difficult due to the late appearance of the cytopathic effect in cell cultures of natural hosts. in the present study, the sensitivity of 4 methods virus isolation from the test cells was compared: the method of long-t ... | 1982 | 6283741 |
| acyclovir in the treatment of simian varicella virus infection of the african green monkey. | acyclovir (9-(2-hydroxyethoxymethyl)guanine) administered as an intramuscular formulation twice daily at a dosage of 100 mg/kg per day prevented the development of disease in african green monkeys inoculated with simian varicella virus. viremia, appearance of a vesicular rash, and elevations of serum transaminases, each indicative of infection, were suppressed by acyclovir treatment. plasma concentrations of acyclovir were measured and showed rising levels after repeated intramuscular injection ... | 1982 | 6285700 |
| sodium and potassium transport in herpes simplex virus-infected cells. | sodium (na+) and potassium (k+) flux in african green monkey kidney cells (vero) was examined following infection by herpes simplex virus type 1 (hsv-1). a decline in the rate of k+ uptake at 5 h post-infection was shown using 86rb+ as a k+ tracer. in contrast, host protein synthesis was inhibited by 3 h post-infection. the decrease in rate of k+ transport to levels 70 to 90% of that of mock-infected cells did not, however, reflect an inability of hsv-1-infected cells to maintain normal intracel ... | 1982 | 6286847 |
| epstein-barr virus induced proteins v: comparison of ebv-specific polypeptides from different virus strains. | ebv-associated polypeptides induced in different epstein-barr virus (ebv)-producing cell lines by the tumor promotor tpa, and from purified ebv particles derived from the same lines were radioactivity labeled and analyzed by immunoprecipitation with human vca+ma+ sera. in virus-producing cells no significant differences in the molecular weight of 35s-methionine-labeled ebv-associated polypeptide patterns could be observed. the analysis 125i-labeled polypeptides from purified virus particles of f ... | 1982 | 6289060 |
| labeling and selective recovery of newly synthesized viral dna from simian virus 40-infected cells incubated with inorganic thiophosphate. | african green monkey cv-1 cells infected with simian virus 40 (sv40) were incubated in the presence of sodium [35s]thiophosphate. the viral dna was prepared and the newly synthesized sv40 dna molecules containing phosphorothioate groups were selectively recovered by affinity chromatography on organomercurial-sepharose columns. the thio-derivatized viral dna occurred in both superhelical (form i) and relaxed circular (form ii) configurations, and it gave the expected cleavage products upon digest ... | 1982 | 6289306 |
| improved yields and assay of simian varicella virus, and a comparison of certain biological properties of simian and human varicella viruses. | studies were performed to define conditions under which propagation, assay and stabilization of the delta herpesvirus (dhv) strain of simian varicella virus might be improved, and to compare biological properties of dhv with those of human varicella zoster virus (vzv). a mycoplasma contaminant was successfully eliminated from the dhv seed virus by treatment with a specific anti-serum. dhv was found to replicate more efficiently in the bs-c-1 line of african green monkey kidney cells than in vero ... | 1982 | 6296181 |
| the influenza virus nucleoprotein synthesized from cloned dna in a simian virus 40 vector is detected in the nucleus. | we obtained dna sequences coding for the nucleoprotein (np) of an influenza a virus by reverse transcription of virion rna with synthetic oligonucleotide primers. terminal sequence analysis showed that the cloned gene contained a full-length copy of the virion rna segment. the np-specific dna was inserted into the late region of a simian virus 40 vector, and the dna recombinant was propagated in the presence of an early simian virus 40 temperature-sensitive mutant helper. infection of african gr ... | 1983 | 6296449 |
| prevalence of neutralizing antibodies to bovid herpesvirus 2 in african wildlife. | a total of 3,470 sera, collected between 1963 and 1980 from 45 different species of wildlife in nine african countries, was examined for virus neutralizing (vn) antibodies to bovid herpesvirus 2. antibodies were demonstrated in 20 species including 15 bovidae, two suidae, hippopotamus (hippopotamus amphibius), giraffe (giraffa camelopardalis) and a green monkey (cercopithecus aethiops); 11 of these species had not been previously recorded as sero-positive. although the significance of neutralizi ... | 1982 | 6296471 |
| a mouse type 2 alu sequence (m2) is mobile in the genome. | the alu and its equivalent families of interspersed repetitive dna sequences have been found in various mammalian genomes. it has been proposed that some of them might move around the genome like known prokaryotic and eukaryotic transposable elements, as most of these sequences are flanked by short direct repeats at both ends. to prove that this is the case, however, one must demonstrate the existence of homologous sequences of dna with and without alu insertion among the genomes of different st ... | 1983 | 6296687 |
| changes in dna binding by purified simian rna polymerase ii under transcribing and nontranscribing conditions. | the interaction of rna polymerase ii from african green monkey liver tissue with sv40 dna was examined by a dnaase protection technique. in the absence of nucleoside triphosphates, simian polymerase binds to nicked, linear sv40 dna and protects 30 bp of binary complex dna from dnaase i digestion. with the addition of nucleoside triphosphates to initiate transcription, polymerase protects 40 bp of the ternary complex dna from dnaase i. thus, a conformational change in either the polymerase, the d ... | 1982 | 6297563 |
| cold adaptation of parainfluenza virus type 3: induction of three phenotypic markers. | in order to attenuate parainfluenza type 3 virus, a wild type strain that was isolated from a child with respiratory disease was adapted to replicate in african green monkey kidney cells at 20 degrees c. replication at 20 degrees c was not a property of the wild type virus. the virus was serially passaged 45 times in the cold, and clones were selected following passage levels 7, 12, 18, and 45. the population of cold-adapted virus was found to be progressively enriched with temperature sensitive ... | 1982 | 6298358 |
| alignment of the genome of monkey b-lymphotropic papovavirus to the genomes of simian virus 40 and bk virus. | we located the origin of dna replication of african green monkey b-lymphotropic papovavirus dna by analyzing pulse-labeled form i dna. with the replication origin used as a reference point, the b-lymphotropic papovavirus genome was aligned with the genomes of simian virus 40 and bk virus from dna homology between specific fragments hybridized under low-stringency conditions. from the results of these experiments, it was possible to deduce the correlation between the physical and functional maps ... | 1983 | 6298468 |
| members of the kpni family of long interspersed repeated sequences join and interrupt alpha-satellite in the monkey genome. | three different members of a family (kpni-family) of interspersed repeated dna sequences were found linked to alpha-satellite sequences in cloned segments of the african green monkey genome. in two of these segments the kpni-family member is over 6 kbp in length and one of them is flanked by alpha-satellite on both sides indicating that it was inserted into a satellite array. hybridization of subcloned portions of the family members to restriction endonuclease digests of monkey and human dna and ... | 1983 | 6298721 |
| effect of acyclovir and presence of cellular and viral thymidine kinase activity in herpes simplex virus infected cells. brief report. | herpes simplex type 1 (hsv-1) infected human lung fibroblasts show a higher viral thymidine kinase activity than infected african green monkey kidney cells. this may partly account for the higher antiviral activity of acyclovir in the former cells. | 1982 | 6299236 |
| detection of clostridium perfringens enterotoxin by tissue culture and double-gel diffusion methods. | culture filtrates of four enterotoxin-producing strains of clostridium perfringens type a produced morphological changes in cells of 10 of 12 established mammalian cell lines tested, and particularly vero (african green-monkey kidney) and mdck (dog kidney) cells. double-gel diffusion and vero-cell tests detected enterotoxin in culture filtrates of, respectively, 29 (28%) and 56 (54%) of 103 strains of c. perfringens tested. the feasibility of cell-culture assays for the routine detection of c. p ... | 1983 | 6302258 |
| deca-satellite: a highly polymorphic satellite that joins alpha-satellite in the african green monkey genome. | three different cloned segments of african green monkey dna that contain alpha-satellite sequences linked to a previously undescribed, distinct monkey satellite (called deca-satellite) are described here. the cloned segments were derived from a monkey dna library in lambda charon 4a that was constructed to select for junctions between alpha-satellite and other dna sequences. the structure of the deca-satellite and of a junction between deca-satellite and alpha-satellite were studied by subclonin ... | 1983 | 6302277 |
| identification of a 50,000 mr protein from rabbit brush border membranes that binds clostridium perfringens enterotoxin. | a protein that binds clostridium perfringens enterotoxin was extracted with np-40 from rabbit intestinal brush border membranes. this protein was partially purified by affinity chromatography on enterotoxin-coupled cnbr-activated sepharose 4b. the molecular weight of this protein was approximately 50,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. affinity-purified samples containing this protein specifically inhibited biological activity of the enterotoxin on ver ... | 1983 | 6303335 |
| preparation of a "functional library" of african green monkey dna fragments which substitute for the processing/polyadenylation signal in the herpes simplex virus type 1 thymidine kinase gene. | fragments of african green monkey (cercopithecus aethiops) dna (3.5 to 18.0 kilobases) were inserted downstream from the thymidine kinase (tk, tk) coding region in ptk206/sv010, a gene construct which lacks both copies of the hexanucleotide 5'-aataaa-3' and contains a simian virus 40 origin of replication, allowing it to replicate in cos-1 cells. no polyadenylated tk mrna was detected in cos-1 cells transfected by ptk206/sv010. the ability of simian dna fragments to restore tk gene expression wa ... | 1983 | 6304500 |
| defects in functional expression of an influenza virus hemagglutinin lacking the signal peptide sequences. | we have investigated the requirement of the signal sequence for expression of influenza virus hemagglutinin (ha). for this purpose we used a recombinant prepared from a late-region deletion mutant of simian virus 40 (sv40) and cloned influenza ha dna; the influenza dna was inserted into the late region of sv40 previously occupied by the deleted sequences coding for sv40 capsid proteins. a simple in-phase deletion was made in the ha dna, resulting in loss of 11 internal amino acids from the 16 am ... | 1983 | 6304718 |
| a new monkey lymphotropic papovavirus: characterization of the virus and evidence of a related virus in humans. | the biological properties of a new lymphotropic papovavirus have been described. the virus replicates only in certain human and monkey b lymphoblastoid cell lines and appears to have a strict requirement for dividing cells in order to grow. human t or null cells do not support its growth. serologic evidence has been presented to show that virtually all primates, including man, are infected with viruses similar or identical to the african green monkey lymphotropic papovavirus. approximately 30% o ... | 1983 | 6304774 |
| influenza virus hemagglutinin expression is polarized in cells infected with recombinant sv40 viruses carrying cloned hemagglutinin dna. | primary cell cultures of african green monkey kidney (agmk) contain polarized epithelial cells in which influenza virus matures predominantly at the apical surfaces above tight junctions. influenza virus glycoproteins were found to be localized at the same membrane domain from which the virus budded. when polarized primary agmk cells were infected with recombinant sv40 viruses containing dna coding for either an influenza virus h1 or h2 subtype hemagglutinin (ha), the ha proteins were preferenti ... | 1983 | 6305510 |
| radioimmunofocus assay for quantitation of hepatitis a virus in cell cultures. | a new method is described for the quantitation of hepatitis a virus in cell cultures, based on the immune autoradiographic detection of foci of infected cells (radioimmunofoci) developing beneath an agarose overlay 14 days after the inoculation of petri dish cultures of continuous african green monkey kidney cells (bs-c-1). the number of foci developing in each culture was linearly related to the dose of hepatitis a virus (either hm-175 or pa-21 strain) inoculated. focus development was prevente ... | 1983 | 6306048 |
| sequence specific cleavage of african green monkey alpha-satellite dna by micrococcal nuclease. | the sequence specificity of micrococcal nuclease complicates its use in experiments addressed to the still controversial issue of nucleosome phasing. in the case of alpha-satellite dna containing chromatin from african green monkey (agm) cells cleavage by micrococcal nuclease in the nucleus was reported to occur predominantly at only one location around position 126 of the satellite repeat unit (musich et al. (1982) proc. natl. acad. sci. usa 79, 118-122). dna control experiments conducted in th ... | 1983 | 6306582 |
| eight different highly specific nucleosome phases on alpha-satellite dna in the african green monkey. | the question of nucleosome phasing on african green monkey (agm) alpha-satellite dna has been addressed by employing a new approach. nucleosome cores were prepared from agm nuclei with micrococcal nuclease, exonuclease iii and nuclease s1. the core dna population derived from alpha-satellite dna containing chromatin was purified from total core dna by denaturation of the dna, reassociation to a low cot value, and hydroxyapatite chromatography to separate the renatured satellite fraction. after e ... | 1983 | 6306583 |
| kpn i family of long interspersed repeated dna sequences in primates: polymorphism of family members and evidence for transcription. | an approximately equal to 2-kilobase-pair-long member (kpn i-ls1) of the african green monkey kpn i family of repeated sequences has been cloned, subjected to sequence analysis, and compared to other family members which are over 6 kilobase pairs (kpn i-alpha 7) and 829 base pairs (kpn i-ret) long. both kpn i-ls1 and kpn i-ret lack sequences found at the ends of the longer family member and their structures resemble those of processed genes. kpm i-ls1 sequences are colinear with part of the long ... | 1983 | 6306655 |
| high efficiency dna-mediated transformation of primate cells. | tissue culture cells from several mammalian species, including three primate lines, were transfected with recombinant vectors carrying escherichia coli xanthine-guanine phosphoribosyltransferase or tn5 aminoglycoside phosphotransferase dominant selectable markers. human hela and sv40-transformed xeroderma pigmentosum cells exhibited stable transformation frequencies of at least 10(-3) (0.1 percent). cv-1, an african green monkey kidney cell line, could be stably transformed with the exceptionall ... | 1983 | 6306768 |
| isolation, propagation, and characterization of a second equine rotavirus serotype. | a rotavirus designated strain h-2 was isolated in primary african green monkey kidney cells from a foal with diarrhea. this cell culture-adapted strain was found to be similar, if not identical, to simian rotavirus (strains mmu18006 and sa-11) and canine rotavirus (strain cu-1) and, in addition, demonstrated a one-way antigenic relationship with five human rotavirus strains (p, b, no. 14, no. 15, and yo) of the third human rotavirus serotype by the plaque reduction neutralization test. this is t ... | 1983 | 6309657 |
| direct isolation in cell culture of human rotaviruses and their characterization into four serotypes. | of 73 rotavirus-positive fecal specimens tested, 39 yielded a human rotavirus that could be cultivated serially in ma104 or primary african green monkey kidney cells or both; 18 were serotyped. four distinct serotypes were identified by plaque reduction or tube neutralization assay or both, and three of these serotypes were the same as those established previously by plaque reduction, using human rotaviruses cultivated by genetic reassortment with a cultivable bovine rotavirus. ten human rotavir ... | 1983 | 6311872 |
| isolation and characterization of an equine rotavirus. | a rotavirus, designated as the h-1 strain, was isolated from a diarrheic foal in primary african green monkey kidney cells and ma104 cells. this cell culture-adapted strain hemagglutinated erythrocytes of human group o, rhesus monkeys, guinea pigs, and sheep. it was found to be similar, if not identical, to porcine rotaviruses (strains osu, ee, and a-580) by plaque reduction neutralization and hemagglutination inhibition tests, and, in addition, it was found to belong to subgroup 1. this equine ... | 1983 | 6313746 |
| nucleosome arrangement in green monkey alpha-satellite chromatin. superimposition of non-random and apparently random patterns. | we have studied the structure of tandemly repetitive alpha-satellite chromatin (alpha-chromatin) in african green monkey cells (cv-1 line), using restriction endonucleases and staphylococcal nuclease as probes. while more than 80% of the 172-base-pair (bp) alpha-dna repeats have a hindiii site, less than 15% of the alpha-dna repeats have an ecori site, and most of the latter alpha-repeats are highly clustered within the cv-1 genome. ecori and hindiii solubilize approximately 8% and 2% of the alp ... | 1983 | 6313939 |
| herpes simplex virus defective genomes: structure of hsv-1 ang defective dna of class ii and encoded polypeptides. | sequence organization and origin of hsv-1 strain angelotti (ang) class ii defective dna (hsv-1 ang ddna1) were examined in detail by establishing physical maps and by molecular cloning. ddna1 consists of concatemers of tandem repeat units in which sequences from the ul region spanning map coordinates 0.37 to 0.415 of standard hsv ang dna are covalently linked to trs/irs sequences. the size of the repeat unit was determined to be about 8.9 kilobase pairs (kb), comprising sequences of 7.3 kb from ... | 1983 | 6315866 |
| male dominance, serotonergic systems, and the behavioral and physiological effects of drugs in vervet monkeys (cercopithecus aethiops sabaeus). | | 1983 | 6316362 |
| antiherpes activity of (e)-5-(2-bromovinyl)- and 5-vinyl-1-beta-d-arabinofuranosyluracil and some other 5-substituted uracil arabinosyl nucleosides in two different cell lines. | of a series of 5-substituted 1-beta-d-arabinofuranosyluracil (5-x-arau) analogues, (e)-5-(2-bromovinyl)-arau(brvarau) and 5-vinyl-arau (varau) were the most potent inhibitors of plaque formation by two herpes simplex virus type 1 (hsv-1) strains in human embryonic lung fibroblast (helf) cell cultures. they were not only more active than 5-methyl-arau (marau, arat) and 5-ethyl-arau (earau), but even more than 1000 times more potent than the 5-fluoro, 5-iodo, 5-formyl and 5-trifluoromethyl (farau, ... | 1983 | 6316849 |
| nucleotide sequence of a reiterated rat dna fragment. | a reiterated component of rat dna was isolated by restriction with hindiii endonuclease and polyacrylamide gel electrophoresis. sequence analysis revealed that the fragment was 179 nucleotides long. unlike the known 370n reiterated rat dna fragment which contained a high m5c content (2.7 mole%), this repetitive element contained a rather low m5c content (0.5 mole%). the present rat repetitive sequence appeared to be of alpha-type as shown by its significant homologies with alpha dna sequences of ... | 1983 | 6317441 |
| live attenuated vaccine for hepatitis a. | the successful development of hepatitis b vaccines has stimulated interest in the active immunoprophylaxis of type a hepatitis. the development of hepatitis a vaccines has been simplified by the ability to propagate hav in tissue culture systems and the availability of animal model systems, including the chimpanzee and certain species of marmosets in which to evaluate safety and efficacy of candidate vaccines. both inactivated and live, attenuated hepatitis a vaccines are possible, but the limit ... | 1983 | 6317493 |
| biologic properties of viable deletion mutants of simian virus 40 (sv40) rescued from the cells of an sv40-induced hamster lymphocytic leukemia. | a lymphocytic leukemia induced by the oncogenic dna simian virus 40 (sv40) in an inbred lsh/sslak syrian golden hamster was evoked to produce infectious sv40 by fusion of the leukemia cells with grivet monkey kidney (gmk) cells and by exposure of the leukemia cells to the chemical inducers mitomycin c and cycloheximide. plaque-purified viable substrains of the rescued sv40 when studied by restriction endonuclease digestion of viral dna were found to contain small deletions within the hind iii re ... | 1983 | 6317936 |
| chromatin structure of simian virus 40-pbr322 recombinant plasmids in cos-1 cells. | to study the nucleoprotein structure formed by recombinant plasmid dna in mammalian cells, nuclei were isolated from cos-1 cells after transfection with a recombinant (pji1) containing pbr322 sequences and a segment of simian virus 40 containing information for a nuclease-sensitive chromatin structure. the nuclei were incubated with dnase i. dna fragments which were the size of linear pji1 dna were isolated, redigested with restriction enzymes, fractionated by electrophoresis, and detected by hy ... | 1983 | 6318089 |
| human hepatitis b vaccine from recombinant yeast. | the worldwide importance of human hepatitis b virus infection and the toll it takes in chronic liver disease, cirrhosis and hepatocarcinoma, make it imperative that a vaccine be developed for worldwide application. human hepatitis b vaccines are presently prepared using hepatitis b surface antigen (hbsag) that is purified from the plasma of human carriers of hepatitis b virus infection. the preparation of hepatitis b vaccine from a human source is restricted by the available supply of infected h ... | 1984 | 6318124 |
| cultivation and subgroup determination of human rotaviruses from egyptian infants and young children. | primary african green monkey kidney cells were more sensitive than primary cynomolgus monkey kidney and ma104 cells for supporting the growth of human rotaviruses detected in diarrheal stools of egyptian infants and young children. in attempts to characterize these egyptian rotaviruses, only 31% of the strains tested in the form of fecal suspensions were identified as subgroup 1 or 2. after one passage in african green monkey kidney cells, 80% of the strains were identified as subgroup 1 or 2. o ... | 1984 | 6321546 |
| lymphotropic papovavirus transformation of hamster embryo cells. | hamster embryo cells were transformed by african green monkey lymphotropic papovavirus (lpv). the transformed cells contained intranuclear t-antigens demonstrable by fluorescent antibody staining with hamster anti-lpv serum. analysis of uncloned and cloned lines of transformed cells for lpv sequences revealed that the viral dna was present as free nonintegrated and integrated genomes; there were approximately 10 copies of free dna and about one to two copies of integrated genomes per cell. the c ... | 1984 | 6321782 |
| a clustering outbreak of hand, foot, and mouth disease caused by coxsackie virus a10. | a clustering outbreak of hand, foot, and mouth disease (hfmd) occurred from july, 1981 to january, 1982 in matsue city and gotsu city, shimane prefecture. thirty-seven patients with clinical hfmd were virologically and serologically examined, and coxsackie virus a10 (ca10) was isolated in 18 patients from vesicles (7/16), throat-swabs (9/31) and feces (6/7). during the period, no ca16 or enterovirus 71 were isolated from hfmd patients or from other diseases such as pharyngitis, febrile diseases, ... | 1983 | 6321911 |
| a simian virus 40-encoded protein of mr 74,000 daltons is structurally related to the capsid proteins of the virus. | we have demonstrated the synthesis of a 74,000-dalton protein (74k protein) in african green monkey kidney cells infected with simian virus (sv)40. the 74k protein was detected late during the lytic cycle. its synthesis was inhibited by arabinosyl cytosine as was the synthesis of the capsid proteins. monospecific antibodies raised against vp1 and vp3 precipitated the structural proteins and the 74k protein. the 74k protein was not found in purified virions. tryptic peptide analysis demonstrated ... | 1983 | 6323496 |
| monkey b-lymphotropic papovavirus dna: nucleotide sequence of the region around the origin of replication. | we have determined the nucleotide sequence of the hindiii-b dna segment of african green monkey b-lymphotropic papovavirus (lpv), which shows a highly restricted host range and whose genome is a 5.1-kilobase-long circular dna. the segment, consisting of 1,123 base pairs, contained the origin of dna replication, the putative control region for early transcription, and the region probably coding for the amino-terminal portion of t antigens. the symmetrical region at the center of replication origi ... | 1984 | 6323745 |
| host cell glucose metabolism during abortive infection by adenovirus type 12. | progeny virions were not produced following infection of an established line of african green monkey kidney (vero) cells with human adenovirus type 12. in addition, viral structural proteins were not synthesized as determined by the immunofluorescent procedure to detect those proteins in infected cells. cytopathology was observed in the virus-infected vero cells, but it was less pronounced and differed in some other aspects to the cytopathic effect of adenovirus type 12 during productive infecti ... | 1984 | 6323929 |
| some extrachromosomal circular dnas containing the alu family of dispersed repetitive sequences may be reverse transcripts. | a 300 base-pair (bp) size class of small polydisperse circular dna (spcdna) isolated from the bsc-1 line of african green monkey kidney cells was cleaved with the restriction endonuclease sau3a, and the resulting fragments (100 to 200 bp) were cloned in bacteriophage m13 mp7. the nucleotide sequence of each of 24 clones containing dna sequences homologous to the alu family of mobile, dispersed, repetitive elements was then determined. analysis of these sequences revealed that many, and perhaps a ... | 1984 | 6325707 |
| structure of extrachromosomal circular dnas containing both the alu family of dispersed repetitive sequences and other regions of chromosomal dna. | small polydisperse circular dna (spcdna) isolated from the bsc-1 line of african green monkey kidney cells was digested with the restriction endonuclease bamhi and cloned in bacteriophage lambda. the resulting library of 25,000 phage was then screened for the presence of the alu family of short interspersed nucleotide sequences, and four of the 100 alu-positive clones were characterized. in summary: (1) all four clones contained regions other than alu that were homologous to the bsc-1 chromosome ... | 1984 | 6325708 |
| abnormal central visual pathways in the brain of an albino green monkey (cercopithecus aethiops). | the visual pathways of an albino green monkey have been studied electrophysiologically and by autoradiographic methods. the monkey had a white coat and pink eyes; it had a strabismus and a nystagmus. when comparisons were made with normal macaque and green monkeys, several abnormalities could be defined. in the retina there was no foveal pit. a whole mount preparation showed a central area of high ganglion cell density in which the ganglion cells were significantly larger than the most central g ... | 1984 | 6330179 |
| uv irradiation induces an activity which stimulates simian virus 40 rescue upon cell fusion. | uv irradiation of african green monkey cells greatly stimulated efficiency of simian virus 40 induction from simian virus 40-transformed syrian hamster cells after cell fusion. the maximum inducing activity was observed at 15 to 20 h after irradiation but remained only transiently. the addition of cycloheximide after uv irradiation eliminated the stimulation of the activity. | 1984 | 6330532 |
| transcription from sv 40-like monkey dna sequences. | the ability of an african green monkey genomic segment, homologous to the regulatory region of sv40, to promote transcription in monkey cells has been investigated. genomic transcripts from cv-1 cells hybridize to both strands of this sv40-like segment. further examination of the promoter potential of the sv40-like segment using expression vectors suggests that the sv40-like region contains at least part of the necessary information for promoting transcription in both directions. in addition, th ... | 1984 | 6330683 |
| altered mrna splicing in monkey cells abortively infected with human adenovirus may be responsible for inefficient synthesis of the virion fiber polypeptide. | messenger rna encoding the fiber protein of the human adenovirus serotype 2 (ad2) capsid is inefficiently translated in abortively infected african green monkey kidney cells. the amount of fiber mrna present in the cytoplasm of abortively infected monkey cells is less than that in productively infected cells by a factor of 5-10 but synthesis of the fiber polypeptide is reduced by a factor of more than 100. evidence from a variety of experiments indicates that the defect does not lie in the trans ... | 1984 | 6330731 |
| the grivet monkey as a model for study of vaginitis. challenge with anaerobic curved rods and gardnerella vaginalis. | the grivet monkey was used as an experimental model for vaginitis. gardnerella vaginalis was used for challenge in one monkey. g. vaginalis plus a long variant (lcr) of a hitherto unclassified anaerobic curved rod (cr) in another 2 monkeys, this lcr alone in 2, a short variant (scr) of cr in 2, and g. vaginalis plus scr in one monkey. all the bacterial strains had been isolated from human vaginal specimens. in the 2 monkeys exposed to g. vaginalis plus lcr, distinct signs of bacterial vaginosis ... | 1984 | 6336134 |
| the influence of iododeoxyuridine pretreatment of various cell cultures on adenovirus multiplication. | treatment of cell cultures with iododeoxyuridine (iudr) before virus inoculation may enhance the subsequent virus multiplication. this effect was studied in seven kinds of cell cultures with seven human adenoviruses from six subgenera. iudr (50 micrograms/ml) was added 24 h after the seeding of cells, left for 2 days, and removed before virus inoculation. iudr had no effect on cell proliferation. viral cytopathic effect was enhanced in many instances by iudr pretreatment. however, virus multipli ... | 1983 | 6343819 |
| electron microscopic study of leprosy in a mangabey monkey (natural infection). | ultrastructural features of the leproma of a) a naturally infected mangabey monkey, and lepromas and liver of b) a passage mangabey monkey, c) a rhesus monkey, d) an african green monkey, and e) a nine-banded armadillo inoculated with leprosy bacilli isolated from the leproma of a naturally infected mangabey monkey were studied by the freeze-etching technique. the size, shape, and ultrastructural features of leprosy bacilli in the phagolysosomes of macrophages in all of these samples were essent ... | 1984 | 6373629 |
| lipids, lipoproteins, and endocrine profiles during pregnancy in the african green monkey (cercopithecus aethiops). | in an attempt to establish relationships between the endocrine and lipid metabolism during pregnancy, the changes in total plasma cholesterol (tpc) and lipoprotein cholesterol that occur during pregnancy in the african green monkey were investigated longitudinally in ten females in relation to the changes in progesterone, estradiol, and fasting insulin concentrations. respective means for tpc, high-density lipoprotein (hdl) cholesterol, and low-density lipoprotein (ldl) plus very low-density lip ... | 1984 | 6381961 |
| mycoplasmal pid: a review of natural and experimental infections. | the present report is a review of data assuming an etiological relationship between pelvic inflammatory disease (pid) and mycoplasma hominis. thus the organism can be isolated from the vagina/cervix more frequently in pid patients than in any other clinical group, i.e., in half to three-fourths of all such cases. one-fourth of pid patients develop a significant antibody response to m. hominis during the course of the disease. the antibody response can be detected by indirect hemagglutination tes ... | 1983 | 6382823 |
| the use of murine monoclonal antibody b2m-01 for detection and purification of human beta 2-microglobulin. | a murine hybridoma cell line was obtained producing monoclonal antibody specific for human beta 2-microglobulin (beta 2m). the antibody reacts strongly with cell-associted human beta 2m, weakly with the cells of the monkey cercopithecus aethiops, but not with any other non-primate cells tested. it is of igg2a isotype, its pi is 6.9-7.1 and dissociation constant of its complex with soluble beta 2m is k = 1.5 x 10(-8) mol/l. the antibody also binds to beta 2m after electrophoretic separation under ... | 1984 | 6394369 |
| alterations in post-transcriptional modification of the y base in phenylalanine trna from tumor cells. | various tumor cells contain chromatographically distinct isoacceptor trna species. to decide whether the tumor-specific species represent an expression of a separate trna gene or only an undermodified form of normal trnaphe, nucleotide sequences of trnaphe isolated from neuroblastoma and normal mouse liver were determined by postlabeling techniques. the results showed identical sequences except for the changes of post-transcriptional modifications in the anticodon loop. normal mouse liver trnaph ... | 1983 | 6405457 |
| in vivo incorporation of drosophila h2a histone into mammalian chromatin. | hybrid prokaryotic/eukaryotic expression vectors have been used to introduce drosophila histone genes into cv-1 african green monkey tissue culture cells. transfection of cv-1 cells with drosophila genes under the control of insect dna promoter sequences results in low level expression of histone genes. on the other hand, when the drosophila h2a gene is juxtaposed downstream from the long terminal repeat sequence of rous sarcoma virus (rsv) expression of the insect gene is considerably more effi ... | 1983 | 6406983 |
| the pathogenesis of trypanosomiasis of the cns. studies on parasitological and neurohistological findings in trypanosoma rhodesiense infected vervet monkeys. | parasitological examinations of the cerebrospinal fluid of 20 vervet monkeys (cercopithecus aethiops), that had been infected with trypanosoma rhodesiense, revealed that the csf was regularly infested with trypanosomes in the early phase of the disease, at the earliest on the 13th day, in most of the animals in the 3rd or 4th week, after infection. follow-up examinations of the csf during the further course of the disease also regularly proved positive for trypanosomes. histological studies in t ... | 1983 | 6407189 |
| restricted repair of aflatoxin b1 induced damage in alpha dna of monkey cells. | we have investigated the processing of adducts formed by covalent binding of aflatoxin b1 (afb1) to dna in confluent cultures of african green monkey cells. repair synthesis elicited by afb1 adducts was deficient in alpha dna sequences compared to that in bulk dna, although the initial levels of modification were the same for these dnas. the removal of the primary initial adduct, afb1-n7-guanine, was deficient in alpha dna and the kinetics of its loss resembled those previously reported for remo ... | 1983 | 6412212 |
| the effect of splinting upon periodontal and pulpal healing after autotransplantation of mature and immature permanent incisors in monkeys. | the effect of splinting upon periodontal and pulpal healing after autotransplantation of teeth with complete and incomplete root formation was studied in 16 green vervet monkeys (cercopithecus aethiops). 2 maxillary incisors were extracted in each monkey and autotransplanted to the contralateral socket. one of these teeth was stabilized with an acrylic splint for either 2 or 6 weeks, while the other incisor was non-splinted. the animals were sacrificed 8 weeks after autotransplantation and the a ... | 1983 | 6418671 |
| methylphenidate, apomorphine, thip, and diazepam in monkeys: dopamine-gaba behavior related to psychoses and tardive dyskinesia. | in eight monkeys (cercopithecus aethiops), previously treated with haloperidol for 4-14 months, we have examined the behavioral effect of: (1) methylphenidate vs apomorphine; (2) 4,5,6,7-tetrahydroisoxazolo-(5,4-c)-pyridin-3-ol(thip, a gaba agonist) vs diazepam; and (3) thip and diazepam in methylphenidate-induced behavior. methylphenidate (0.5-5.0 mg/kg) and apomorphine (0.1-0.5 mg/kg) both increased locomotion, but otherwise exhibited different behavioral profiles. methylphenidate induced repe ... | 1984 | 6420823 |
| measurement of apolipoprotein a-i concentration in nonhuman primate serum by enzyme-linked immunosorbent assay (elisa). | a sensitive and specific enzyme-linked immunosorbent assay (elisa) for nonhuman primate serum apolipoprotein a-i (apoa-i) is described. the assay is a noncompetitive, sandwich elisa in which polystyrene microtiter plates were used with purified, monospecific goat anti-monkey apoa-i antibodies adsorbed on the wells. the serum samples were added to the coated wells, incubated, and after washing, antibodies conjugated to horseradish peroxidase were added. after further washing, the bound label was ... | 1983 | 6421975 |
| x-linked, polymorphic genetic variation of thyroxin-binding globulin (tbg) in baboons and screening of additional primates. | x-linked polymorphic variation of thyroxin-binding globulin (tbg) is observed in several human groups. isoelectric focusing of plasma samples labeled in vitro with [125i]thyroxin, followed by autoradiography, also reveals genetically determined polymorphic electrophoretic variation in baboon tbg. the protein detected by this method in baboon plasma is immunologically similar to human tbg and is distinct from the other thyroxin-binding proteins, albumin and prealbumin. the isoelectric patterns of ... | 1984 | 6424646 |
| effect of trans-unsaturated fats on experimental atherosclerosis in vervet monkeys. | vervet monkeys ( ceropithecus aethiops pygerethrus ) were placed on semipurified diets containing 14% fat of which 3.2 or 6.0% was present as trans-unsaturated fatty acid (t-fa). two groups were fed the high and low levels of t-fa for a year and two others were fed t-fa for 6 months and then returned to the control diet for 6 months more. one other group was fed the control diet for a year. the control diet contained 14% fat which was a mixture of 72% olive oil and 28% corn oil. there were no si ... | 1984 | 6426484 |
| influence of root development on periodontal and pulpal healing after replantation of incisors in monkeys. | the influence of root development on periodontal and pulpal healing after replantation was examined in 30 green vervet monkeys (cercopithecus aethiops) in which a total of 50 teeth were replanted. maxillary central incisors with different stages of root development (immature, young mature and mature root formation) were replanted with untreated pulps or after root canal treatment with gutta percha and kerr sealer. the extra-alveolar period in all groups was 18 min. the animals were sacrificed 8 ... | 1984 | 6434451 |
| ultraviolet irradiation of monkey cells enhances the repair of dna adducts in alpha dna. | excision repair of bulky adducts in alpha dna of african green monkey cells has previously been shown to be deficient relative to that in the overall genome. we have found that u.v. irradiation of these cells results in the enhanced removal of both aflatoxin b1 (afb1) and acetylaminofluorene (aaf) adducts from the alpha dna sequences without affecting repair in the bulk of the dna. the degree of enhanced removal of afb1 is dependent upon the u.v. dose and the time interval between irradiation an ... | 1984 | 6435903 |
| the distribution of serum high density lipoprotein subfractions in non-human primates. | the ultracentrifugal flotation patterns in 1.2 g/ml solvent and ultracentrifugal gradient distribution of high density lipoproteins (hdl) from the primates--human, apes and monkeys--were determined, with emphasis on the gorilla species of apes and rhesus monkeys. diets for non-human primates were commercial chow, which is low in cholesterol. molecular weights and protein, cholesterol, phospholipid and triglyceride compositions of various density fractions were determined on human, gorilla and rh ... | 1984 | 6438429 |