| surfactant protein d inhibits mite-induced alveolar macrophage and dendritic cell activations through tlr signalling and dc-sign expression. | surfactant protein d (sp-d), a secreted pattern recognition molecule associated with pulmonary innate immunity, has been shown to mediate the clearance of pathogens in multiple ways. however, how sp-d interacts with alveolar macrophages (ams) and dendritic cells (dcs) during allergen exposure remains unclear. | 2010 | 20205699 |
| clinical efficacy of house dust mite-specific immunotherapy in asthmatic children. | immunotherapy has been widely used in the treatment of allergic diseases. we evaluated the clinical efficacy of specific immunotherapy with extracts of dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df) in children with asthma. | 2010 | 20225533 |
| flaky tail mouse denotes human atopic dermatitis in the steady state and by topical application with dermatophagoides pteronyssinus extract. | the barrier abnormality, a loss-of-function mutation in the gene encoding filaggrin (flg), which is linked to the incidence of atopic dermatitis (ad), is a recently discovered but important factor in the pathogenesis of ad. flaky tail (flg(ft)) mice, essentially deficient in filaggrin, have been used to investigate the role of filaggrin on ad. however, the relevancy of flg(ft) mice to human ad needs to be determined further. in this study, we observed the clinical manifestations of flg(ft) mice ... | 2010 | 20304960 |
| [the correlative study between skin prick test with antigens and nasal provoking test in patients with allergic rhinitis]. | to study the distribution of allergens in our district and the correlation between skin prick test with antigen and nasal provoking test in patients with allergic rhinitis. | 2010 | 20306852 |
| house-dust mite nasal provocation: a diagnostic tool in perennial rhinitis. | in perennial allergic rhinitis (par), the skin-prick test (spt) is a good diagnostic tool to identify the specific allergens. a nasal provocation test (npt) is used to identify allergens and to confirm the diagnosis. the aim of this study was to determine the optimal cutoff values of symptom and peak nasal inspiratory flow (pnif) changes after dust-mite npt for predicting par. we also studied the relationship of the changes of symptoms in npt and the wheal size of spt. | 2010 | 20338112 |
| a comparison of in vitro potency between european and mexican allergen extracts and us (cber/fda) reference extracts. | the most important allergen manufacturers are based in europe and in the us. in some countries local products are also sold. no comparison between european, us and local products has been made until now. | 2010 | 20338683 |
| der-p2 ( dermatophagoides pteronyssinus) allergen-like protein from the hard tick ixodes ricinus - a novel member of ml (md-2-related lipid-recognition) domain protein family. | expression of the gene encoding der-p2 allergen-like protein in the castor bean tick ixodes ricinus is induced by blood intake. tick der-p2 allergen-like protein belongs to a diverse family of ml proteins that includes major allergens of house dust mites, human md-2 or similar proteins from drosophila melanogaster. in ticks, genes encoding proteins belonging to the ml protein family were identified, but their protein products have not been characterized yet. | 2010 | 20388233 |
| recombinant fusion proteins assembling der p 1 and der p 2 allergens from dermatophagoides pteronyssinus. | fusion proteins assembling multiple allergens can be engineered by recombinant dna technologies in order to produce tools for diagnostic and immunotherapeutic purposes. herein, we developed and characterized chimeras assembling der p 1 and der p 2 allergens as potential candidate vaccines against house dust mite allergy. | 2010 | 20407270 |
| [acarological faunistic and statistical analysis of dust from various compartments of human dwellings in north-western poland]. | the aim of this paper was to compare the species composition of mites in dust samples collected at different compartments of human dwellings of north-western (nw) poland. in 30 urban apartments (szczecin, police, pyrzyce and łobez) and rural quarters (przelewice, brzesko szczecińskie, bylice, krasne, kakolewice, wierzchowo, przeradz, kłodzino) from nw poland, 150 samples of dust were collected at 5 locations: bed area, bedroom carpet, sitting area in the living room, hall carpet, and kitchen flo ... | 2010 | 20450008 |
| presence and density of domestic mites in the microenvironment of mite-sensitive dogs with atopic dermatitis. | this study was designed to investigate the presence and density of domestic mites (dms) in households with atopic dogs sensitive to these mites (group a; n=20), in households with clinically healthy, nonatopic dogs (group b; n=20) and in households without pets (group c; n=25). dust samples were vacuum-collected from the owner mattress (all groups) and dog sleeping area (groups a and b) or living room couch (group c) on four consecutive occasions, reflecting the four seasons of the year. dms wer ... | 2010 | 20456719 |
| molecular mimicry as the key to the dominance of the house dust mite allergen der p 2. | evaluation of: trompette a, divanovic s, visintin a et al. allergenicity resulting from functional mimicry of a toll-like receptor complex protein. nature 457, 585-588 (2009). the majority of complex sources of allergen contain a small number of dominant allergens that bind at least half of the ige antibodies of allergic subjects. for the house dust mite dermatophagoides pteronyssinus, these allergens are der p 1 and der p 2. there is evidence that the cysteine-protease activity of der p 1 impar ... | 2009 | 20477001 |
| tlr2 agonists enhance cd8+foxp3+ regulatory t cells and suppress th2 immune responses during allergen immunotherapy. | pam3csk4, a synthetic tlr2 ligand, has been shown to expand cd4+ regulatory t cells (treg cells). less is known about the function of cd8+ treg cells than about the function of cd4+ treg cells generated during allergen-specific immunotherapy (it). this study investigated whether dermatophagoides pteronyssinus-specific it could expand the cd8+cd25+foxp3+ treg population and whether pam3csk4 could enhance the treg population. pbmcs were isolated from healthy control subjects and from mite-sensitiv ... | 2010 | 20483759 |
| skin prick test evaluation of dermatophagoides pteronyssinus diagnostic extracts from europe, mexico, and the united states. | previous food and drug administration (fda)-approved enzyme-linked immunosorbent assay testing of dermatophagoides pteronyssinus diagnostic extracts showed potencies of 36% to 44% for 3 european extracts relative to the fda standard (10,000 au/ml). | 2010 | 20486333 |
| a survey of serum specific-lge to common allergens in primary school children of taipei city. | environmental factors and eating habits have had a significant impact on the increased sensitization to allergens in children. this study investigated changes in common allergen sensitivities among children in taipei city, taiwan. a total of 142 primary schools in taipei city, which included 25,094 students aged 7-8 years, were surveyed using an isaac questionnaire to screen for allergies. for positive responders, serum allergen-specific ige was confirmed using the pharmacia cap system. a total ... | 2010 | 20527509 |
| skin test reactivity to indoor allergens correlates with asthma severity in jeddah, saudi arabia. | abstract : background : there is increased emphasis on the role of indoor allergens in asthma. objective : to examine the spectrum of skin test reactivity (sensitization) to indoor allergens and its correlation with asthma severity in jeddah, saudi arabia. methods : asthmatic patients referred to the allergy clinic at king abdulaziz university hospital (kauh) in jeddah were studied. measures of clinical severity were adopted from national and international asthma guidelines. the degree of sensit ... | 2006 | 20529215 |
| comparison of the skin-prick test and phadia immunocap as tools to diagnose house-dust mite allergy. | when the skin-prick test (spt) and in vitro test such as immunocap assay are performed simultaneously, results do not always coincide in some patients. our objectives, therefore, were (1) to assess differences in allergic test results according to age group and (2) to establish appropriate guidelines for diagnosing mite allergy according to age. | 2010 | 20537291 |
| rhinitis is also common in infants with asthma. | the aim of this study was to assess the frequency of rhinitis in asthmatic infants. a cross-sectional study was conducted using clinical data obtained from a standardized allergy work-up form that includes specific questions on common allergic diseases. asthmatic patients were seen at the first visit to the pediatric allergy unit, from january 2001 to january 2006, were selected for analysis. diagnosis of allergic rhinitis was based on the presence of two or more nasal symptoms (sneezing, itchin ... | 2010 | 20548130 |
| geographical variations in the prevalence of atopic sensitization in six study sites across canada. | geographical variations in atopic sensitization in canada have not been described previously. this study used the standardized protocol of the european community respiratory health survey-1 (ecrhs-1) to investigate the distribution and predictors of atopic sensitization in six sites across canada and to compare the results with some ecrhs-1 centers. | 2010 | 20557300 |
| association of indoor air pollution with rhinitis symptoms, atopy and nitric oxide levels in exhaled air. | exposure to particulate matter (pm) outdoors can induce airway inflammation and exacerbation of asthma in adults. however, there is limited knowledge about the effects of exposure to indoor pm. the aim of this study was to investigate the association of exposure to indoor sources of pm with rhinitis symptoms, atopy and nitric oxide in exhaled air (feno) as a measure of airway inflammation. | 2010 | 20559007 |
| efficacy of high-dose sublingual immunotherapy in children allergic to house dust mites in real-life clinical practice. | the aim of the study was to evaluate the efficacy and safety of high-dose mite sublingual immunotherapy (slit) administered in children with allergic rhinitis in real-life clinical practice. moreover, we analysed the clinical course of asthma severity. | 2010 | 20570032 |
| iκb kinase β inhibitor imd-0354 suppresses airway remodelling in a dermatophagoides pteronyssinus-sensitized mouse model of chronic asthma. | nuclear factor (nf)-κb is a transcription factor that regulates cytokine and chemokine production in various inflammatory diseases, including bronchial asthma. iκb kinase (ikk) β is important for nf-κb activation in inflammatory conditions, and is possibly related to airway remodelling. thus, inhibition of the ikkβ-nf-κb pathway may be an ideal strategy for the management of airway remodelling. | 2011 | 20573155 |
| enzymatic activities of allergen extracts from three species of dust mites and cockroaches commonly found in korean home. | allergen extracts from dust mites and cockroaches commonly found in korean homes were used to evaluate their enzymatic activity as they are believed to influence allergenicity. allergen extracts were prepared from 3 dust mite species (dermatophagoides farinae, d. pteronyssinus, and tyrophagus putrescentiae) and 3 cockroach species (blattella germanica, periplaneta americana, and p. fuliginosa) maintained in the korea national arthropods of medical importance resource bank. proteins were extracte ... | 2010 | 20585532 |
| therapeutic effects of baicalein on atopic dermatitis-like skin lesions of nc/nga mice induced by dermatophagoides pteronyssinus. | the present study was conducted to investigate the effects of baicalein (be), which is hydrolyzed product of baicalin (ba), on atopic dermatitis (ad). ad was induced in nc/nga mice by dpe treatment. be hydrogels treatment reduced the levels of skin severity scores. be hydrogels treatment also decreased inflammatory cytokines such as tnf-alpha, il-6, and its level in the serum. be hydrogels treatment elevated ifn-gamma level in the spleenocyte culture supernatant. cell numbers in the skin positiv ... | 2010 | 20621172 |
| perennial allergen-specific immunoglobulin e levels among inner-city elderly asthmatics. | the role of allergen sensitization has been well established among children and young adults with asthma. some studies have suggested that allergens play a less important role among older patients with asthma. however, whether older asthmatics have a lower prevalence of allergen sensitization than younger adults remains controversial. the aim of this study was to access the prevalence of sensitization to indoor allergens in older compared to younger inner-city adults with persistent asthma. | 2010 | 20662744 |
| observational study of the safety of an ultra-rush sublingual immunotherapy regimen to treat rhinitis due to house dust mites. | ultra-rush regimens for administering sublingual immunotherapy to patients with allergies are becoming more widespread. we aimed to assess treatment safety for patients with allergic rhinitis with or without asthma caused by dermatophagoides house dust mites. | 2011 | 20664280 |
| analysis of expression and amino acid sequence of the allergen mag 3 in two species of house dust mites-dermatophagoides farinae and d. pteronyssinus (acari: astigmata: pyroglyphidae). | in the 90's of the xx century, 2 new and important allergens of house dust mites mites were cloned and sequenced: mag 1 and mag 3. however, the second allergen has been identified to date only in extracts of dermatophagoides farinae [df ]. in this work, we aimed to detect expression of this important allergen and for the first time analyze to the amino acid sequence in other species of house dust mite - dermatophagoides pteronyssinus [dp ]. we were able to confirm the expression of allergen mag ... | 2010 | 20684479 |
| assessment of allergen sensitization in a general population-based survey (european community respiratory health survey i). | exposed to a common environment, the ige-mediated immune response differs, for instance, among sensitized subjects, some of them reacting toward one allergen (monosensitized) whereas others are sensitized to a wide array of allergens (polysensitized). however, a better phenotypic characterization is needed for epidemiologic studies. using the data collected during the ecrhs i (european community respiratory health survey), several assessments of skin prick tests and serum-specific ige to identif ... | 2010 | 20702109 |
| impact of ige-mediated hypersensitivity on nasal mucociliary clearance. | to evaluate the effect of ige-mediated hypersensitivity on mucociliary clearance time (mcct) and clinical severity, as indicated by total nasal symptoms score (tnss) and peak expiratory flow index (pefi). | 2010 | 20713757 |
| the presence of serum anti-ascaris lumbricoides ige antibodies and of trichuris trichiura infection are risk factors for wheezing and/or atopy in preschool-aged brazilian children. | the elucidation of factors that trigger the development of transient wheezing in early childhood may be an important step toward understanding the pathogenesis of asthma and other allergic diseases later in life. transient wheezing has been mainly attributed to viral infections, although sensitisation to aeroallergens and food allergens may occur at an early age. in developing countries, intestinal helminthic infections have also been associated with allergy or atopy-related disorders. | 2010 | 20731833 |
| induction of il-6 and il-8 by house dust mite allergen der p1 in cultured human nasal epithelial cells is associated with par/pi3k/nfkappab signaling. | the mechanism of action involved in how dermatophagoides pteronyssinus (der p) 1 initiates the nasal allergic cascade is poorly understood. | 2010 | 20733339 |
| airway hyperresponsiveness is associated with total serum immunoglobulin e and sensitization to aeroallergens in korean adolescents. | allergen sensitization and airway hyperresponsiveness (ahr) are the most important characteristics of bronchial asthma and their correlation has been speculated. | 2010 | 20812252 |
| clinical and immunologic effects of sublingual immunotherapy on patients with allergic rhinitis to house-dust mites: 1-year follow-up results. | there have been no data on sublingual immunotherapy (slit) in asian patients with allergic rhinitis (ar) sensitized to house-dust mites (hdms). this study aimed to investigate the efficacy and immunologic change after 12 months of slit in korean patients. | 2010 | 20819465 |
| sensitization to dermatophagoides, blomia tropicalis, and other mites in atopic patients. | house dust mites (hdms) are important causes of persistent allergic diseases, such as asthma and rhinitis. various types of mites are found in the house dust of many countries, including israel. | 2010 | 20846087 |
| [dynamical changes of lung function and immunologic markers in asthmatic children receiving specific immunotherapy with standardized house dust mite extract]. | to study the effects of specific immunotherapy with standardized house dust mite extract on the lung function and immunologic markers in children with asthma. | 2010 | 20849721 |
| identification of neryl formate as the airborne aggregation pheromone for the american house dust mite and the european house dust mite (acari: epidermoptidae). | the american house dust mite, dermatophagoidesfarinae hughes, and european house dust mite, dermatophagoides pteronyssinus trouessart, are major pests of medical importance throughout the developed world, causing atopic diseases such as asthma, rhinitis, and atopic dermatitis. previous studies in our laboratory have shown that the behavioral responses of house dust mites toward volatiles from food sources could be assessed using a y-tube olfactometer assay. the current study used this y-tube ass ... | 2010 | 20939374 |
| t regulatory cells in cord blood--foxp3 demethylation as reliable quantitative marker. | regulatory t-cells (tregs), characterized as cd4+cd25(hi) t-cells expressing foxp3, play a crucial role in controlling healthy immune development during early immune maturation. recently, foxp3 demethylation was suggested to be a novel marker for natural tregs in adults. in cord blood, the role and function of tregs and its demethylation is poorly understood. we assessed foxp3 demethylation in cord blood in relation to previously used treg markers such as cd4+cd25(hi), foxp3 mrna, protein expres ... | 2010 | 20967272 |
| a 15-year-old boy with anterior chest pain, progressive dyspnea, and subcutaneous emphysema of the neck. | we describe the case of an adolescent who was admitted to the hospital because of sudden occurrence of chest pain, dyspnea and subcutaneous emphysema. on admission, physical examination revealed subcutaneous crepitations in the superior part of the rib cage, and auscultation of the chest showed widespread wheezing. the radiological assessment confirmed the diagnosis of pneumomediastinum and pneumothorax. a follow-up ct scan performed one week after the admission showed almost complete resolution ... | 2009 | 20975797 |
| evaluation of serum specific ige for the diagnosis of allergic rhinitis with multi-allergens. | objective evaluation of allergic rhinitis (ar) requires in vivo and in vitro tests. in vitro tests are important to assist or ensure the main allergens in multi-allergen-sensitive patients. the aim of this study was to evaluate the utility of serum specific ige (sige) in the diagnosis of ar patients with multi-allergens in the chinese population. | 2010 | 21034593 |
| rapid immunochromatographic measurement of specific tear immunoglobulin e in moderate to severe cases of allergic conjunctivitis with immfast check j1 in the spring. | to evaluate whether immfast check j1, a new commercial immunochromatographic test for the measurement of specific immunoglobulin (ig) e, could be used as a screening test for the diagnosis of allergic conjunctivitis. | 2011 | 21045641 |
| [compliance and safety evaluation of subcutaneous versus sublingual immunotherapy in mite-sensitized patients with allergic rhinitis]. | to investigate the safety profile of subcutaneous immunotherapy (scit) versus sublingual immunotherapy (slit) in patients with allergic rhinitis (ar) caused by house dust mites. the treatment compliance and related factors were also evaluated. | 2010 | 21055319 |
| identification of astigmatid mites using its2 and coi regions. | identification of astigmatid mites based on their morphological characteristics is difficult because of the similarity of their organs, especially in immature mites. the ribosomal second internal transcribed spacer (its2) and the mitochondrial cytochrome oxidase subunit i (coi) regions are highly conserved in the eukaryotes and are usually used as barcodes. the its2 and coi regions of six species of astigmatid mites (aleuroglyphus ovatus, blomia tropicalis, dermatophagoides farinae, dermatophago ... | 2010 | 21072538 |
| dermatophagoides pteronyssinus 2 regulates nerve growth factor release to induce airway inflammation via a reactive oxygen species-dependent pathway. | group 2 allergen of dermatophagoides pteronyssinus 2 (der p2) induces airway inflammation without protease activity, and elevated nerve growth factor (ngf) levels are also found in this inflammation. how the allergen der p2 regulates ngf release via reactive oxygen species (ros) to induce inflammation remains unclear. in the present study, intratracheal administration of der p2 to mice led to inflammatory cell infiltration, mucus gland hyperplasia, and ngf upregulation in the bronchial epitheliu ... | 2010 | 21097523 |
| clinical and immunological features of patients with atopy and concomitant htlv-1 infection. | human t-cell lymphotropic virus type 1 (htlv-1) induces an exacerbated type 1 immune response characterized by high spontaneous ifn-γ and tnf-α production. allergic rhinitis and asthma are associated with the type 2 immune response, with elevated secretion of il-4 and il-5. the aim of this study was to characterize the immune response in atopic htlv-1 carriers. the cytokine profile of atopic htlv-1 carriers (n = 10; all females) was compared with that of non-atopic htlv-1 carriers (n = 14; 9 fem ... | 2010 | 21140101 |
| [usefulness of total ige in predicting positive allergen specific ige tests in korean subjects]. | total ige levels in allergic patients tend to be higher than those in healthy individuals. we evaluated the usefulness of total ige levels in predicting positive results of allergen specific iges in multiple allergen simultaneous tests. | 2010 | 21157154 |
| structural and immunologic cross-reactivity among filarial and mite tropomyosin: implications for the hygiene hypothesis. | the hygiene hypothesis suggests that parasitic infection modulates host immune responses and decreases atopy. other data suggest parasitic infections may induce allergic responsiveness. | 2010 | 21185070 |
| hypersensitivity to house dust mite and cockroach is the most common allergy in north of iran. | asthma and allergic rhinitis are among the most common diseases in the world. | 2010 | 21189446 |
| role of pollen allergy in taiwanese patients with allergic rhinitis. | pollen allergy is believed to be less common in east asia, latin america, and tropical areas. the purpose of this study was to understand the role of pollen allergy in taiwan. | 2010 | 21195885 |
| allergy in the tropics: the impact of cross-reactivity between mites and ascaris. | allergic diseases and nematode infections such as ascariasis are important health problems in underdeveloped tropical countries. the co-exposure to ascaris lumbricoides and the domestic mites blomia tropicalis and dermatophagoides pteronyssinus induces a strong th2 and immunomodulatory responses that can modify the natural history of both diseases. an associate phenomenon of these particular environmental conditions is cross reactivity between mite and ascaris allergens. we demonstrated a high i ... | 2011 | 21196284 |
| patterns of skin prick test positivity in allergic patients: usefulness of a nationwide spt chart review. | background: a previous survey on allergens used by mexican allergists in their skin prick test (spt) panel showed wide variation. humidity varies in different zones of mexico. this might lead to differences in natural exposure and allergic sensitisation throughout the country. we aim to describe the spt sensitivity patterns in the different climatic zones in mexico and to show the usefulness of a structured spt chart-review including multiple clinics in obtaining these allergen sensitisation pat ... | 2011 | 21216084 |
| detection of allergenic compounds using an il-4/luciferase/cns-1 transgenic mice model. | the interleukin-4 (il-4) signaling cascade has been identified as a potentially important pathway in the development of allergies. the principal objective of this study was to produce novel transgenic (tg) mice harboring the luciferase gene under the control of the human il-4 promoter and the enhancer of il-4 (cns-1), in an effort to evaluate three types of allergens including a respiratory sensitizer, vaccine additives, and crude extracts of natural allergens in vivo. a new lineage of tg mice w ... | 2011 | 21252390 |
| high allergen-specific serum immunoglobulin e levels in nonatopic west highland white terriers. | human and canine atopic dermatitis (ad) share an association with ige specific to environmental allergens, but few studies have evaluated serum allergen-specific ige in nonatopic dogs. this study compared serum allergen-specific ige levels in 30 atopic and 18 nonatopic west highland white terriers. atopic dermatitis was confirmed using standard criteria. nonatopic dogs were over 5 years of age and had no clinical signs or history of ad. serum allergen-specific ige levels were measured with aller ... | 2011 | 21265887 |
| sodium sulfite aggravated allergic sensitization and airway inflammation in mite allergen sensitized balb/c mice. | sulfur dioxide is a typical air pollutant. sulfite, which is formed at the bronchial mucosa from inhaled sulfur dioxide, might play a role in the exacerbation of asthma. in this study, we investigated the effects of sodium sulfite and its interaction with a house dust mite (<italic>dermatophagoides pteronyssinus</italic>, der p) on allergic sensitization and airway inflammation. balb/c mice were divided into four groups: control (n = 10), mite intranasal (min, n = 12), sodium sulfite intranasal ... | 2011 | 21300691 |
| production and immunogenicity of hypoallergenic codon-optimized dna vaccine encoding mature der p 1 allergen. | genetic vaccination with plasmid dna encoding allergens is a promising potential approach for the treatment or prevention of allergy. nonetheless, because the allergens expressed can display immunoglobulin (ig) e reactivity, methods to deliver hypoallergenic variants can minimize the risk of type 2 helper (t(h)2) cell priming after dna immunization. | 2010 | 21313999 |
| allergy-related outcomes in relation to serum ige: results from the national health and nutrition examination survey 2005-2006. | the national health and nutrition examination survey (nhanes) 2005-2006 was the first population-based study to investigate levels of serum total and allergen-specific ige in the general us population. | 2011 | 21320720 |
| effects of microwave radiation on house dust mites, dermatophagoides pteronyssinus and dermatophagoides farinae (astigmata: pyroglyphidae). | dermatophagoides pteronyssinus and dermatophagoides farinae mites are commonly found in house dust, and are important sources of allergens affecting humans. various approaches to killing the mites have been examined. this study investigated the mortalities of adult mites exposed to 2,450 mhz microwave radiation produced by 3 ovens at various exposure times and power settings. the ovens all had 3 power settings. the average maximum water temperatures generated at high, medium and low power settin ... | 2010 | 21329308 |
| factors for high-risk asthma in taiwanese children. | asthma is one of the major causes of death in otherwise healthy young individuals. however, many of these deaths may have been prevented by more aggressive treatment. to determine factors correlated with a high risk of death in taiwanese children with atopic asthma. | 2010 | 21337908 |
| non-proteolytic house dust mite allergen, der p 2, upregulated expression of tight junction molecule claudin-2 associated with akt/gsk-3ß/ß-catenin signaling pathway. | non-proteolytic group 2 allergen, der p 2 (dp2) is known as a major allergen derived from house dust mite dermatophagoides pteronyssinus.paracellular epithelial barrier, being composed of a number of tight junction (tj) molecules, plays pivotal roles in resistance of pathogen invading. however, whether dp2 affects epithelial tj molecules is unclear. therefore, we aimed to investigate the effects of dp2 on epithelial tj molecules, and the mechanism by which expression of junction molecules is reg ... | 2011 | 21344483 |
| strain-dependent genomic factors affect allergen-induced airway hyper-responsiveness in mice. | background asthma is etiologically and clinically heterogeneous, making the genomic basis of asthma difficult to identify. objectives we exploited the strain-dependence of a mouse model of allergic airway disease to identify different genomic responses in the lung. methods balb/cj and c57bl/6j mice were sensitized with house dust mite allergen der p 1 without exogenous adjuvant, then given a single der p 1 challenge. allergic inflammation, serum antibody titers, mucous metaplasia, and airway hyp ... | 2011 | 21378263 |
| ige cross-reactivity between ascaris lumbricoides and mite allergens: possible influences on allergic sensitization and asthma. | nematode infections such as ascariasis are important health problems in underdeveloped countries, most of them located in the tropics where environmental conditions also promote the perennial co-exposure to high concentrations of domestic mite allergens. allergic diseases are common, and most of patients with asthma exhibit a predominant and strong ige sensitization to mites. it is unknown whether co-exposure to ascaris lumbricoides and the domestic mites blomia tropicalis and dermatophagoides p ... | 2011 | 21388422 |
| elevation of ige in children with sickle cell disease is associated with doctor diagnosis of asthma and increased morbidity. | a doctor's diagnosis of asthma is associated with increased morbidity (pain and acute chest syndrome [acs]) among children with sickle cell disease (scd). an association between ige levels and asthma and morbidity has not been investigated in children with scd. | 2011 | 21388662 |
| effect of chronic mild stress on serotonergic markers in the skin and brain of the nc/nga atopic-like mouse strain. | atopic eczema is often worsened by stress. while acute stress is associated with increased turnover of serotonin (5-hydroxytryptamine; 5-ht), chronic stress causes a decrease. in chronic stress, there is a decrease of the 5-ht1a receptor (r)- and an increase in the 5-ht2ar-responsiveness to 5-ht. in the present study, the impact of chronic mild stress on the expression of 5-ht1a and 5-ht2a receptors and serotonin transporter protein (sert) was investigated in eczematous skin and brain of atopic- ... | 2011 | 21400247 |
| [change of th17 and expression of ror?t in a murine model of allergic rhinitis after a stimulation of allergen and corticosteroid]. | to study the effects of allergen and corticosteroid on t help 17 (th17) and orphan nuclear receptor gammat (ror?t) in allergic-rhinitis mice. | 2011 | 21418913 |
| evaluation of patch test with airbone allergic agents in patients with atopic dermatitis. | atopic dermatitis is an inflammatory skin disease that can be triggered by many factors. several reports confirm the role of airborne allergic agents as aggravating or triggering factors. the patch test with airborne allergic agents or the atopy patch test was suggested to evaluate the role of these allergens in atopic dermatitis. | 2011 | 21437520 |
| changes of alpha1-antitrypsin levels in allergen-induced nasal inflammation. | alpha1-antitrypsin (aat) is the main inhibitor of human neutrophil elastase, and plays a role in counteracting the tissue damage caused by elastase in local inflammatory conditions. the study evaluated the involvement of aat in nasal allergic inflammation. | 2011 | 21461061 |
| mite allergen decreases dc-sign expression and modulates human dendritic cell differentiation and function in allergic asthma. | house dust mites (hdms) induce allergic asthma in sensitized individuals, although how hdms activate immature mucosal dendritic cells (dcs) to render the t helper cell type 2 (th2)-mediated immune response is unclear. in this study, our results showed a significant calcium-dependent lectin binding of dermatophagoides pteronyssinus (der p) extracts to dc-specific intercellular adhesion molecule-3-grabbing nonintegrin (dc-sign), the c-type lectin receptors (clrs) of dcs. moreover, monocyte-derived ... | 2011 | 21471959 |
| concentrations of plasminogen activator inhibitor-1 (pai-1) and urokinase plasminogen activator (upa) in induced sputum of asthma patients after allergen challenge. | urokinase plasminogen activator (upa) and its inhibitor (pai-1) are involved in tiisue remodeling and repair processes associated with acute and chronic inflammation. the aim of the study was to evaluate the effect of allergen challenge on concentration of upa and pai-1 in induced sputum of house dust mite allergic asthmatics (hdm-aas). thirty hdm-aas and ten healthy persons (hcs)were recruited for the study. in 24 hdm-aas bronchial challenge with dermatophagoides pteronyssinus (dp) and in 6 hdm ... | 2010 | 21478092 |
| contact and fumigant toxicity of cinnamaldehyde and cinnamic acid and related compounds to dermatophagoides farinae and dermatophagoides pteronyssinus (acari: pyroglyphidae). | toxicities of (e)-cinnamaldehyde and (e)-cinnamic acid and their 41 structurally related compounds to adult dermatophagoides farinae hughes and dermatophagoides pteronyssinus trouessart (acari: pyroglyphidae) were examined using fabric-circle contact plus fumigant and vapor-phase mortality bioassays. results were compared with those of two acaricides, benzylbenzoate and dibutyl phthalate. in contact plus fumigant mortality bioassays, the most toxic compounds were (e)-cinnamaldehyde, methyl (e)-c ... | 2011 | 21485375 |
| european and mexican vs us diagnostic extracts of bermuda grass and cat in skin testing. | laboratory testing of various diagnostic extracts has shown lower potencies for several european and mexican extracts relative to the us food and drug administration (fda) reference (10,000 bau/ml). quantitative skin prick testing (qspt) with dermatophagoides pteronyssinus extracts have previously shown a similar picture. | 2011 | 21530875 |
| mite exposure in a spanish mediterranean region. | background: knowledge of the domestic mite fauna and allergen levels is important for a correct diagnosis and treatment of mite allergy. our objectives were to describe the domestic mite fauna in the region of murcia, spain, to quantify mite allergens in dust samples obtained from mattresses of this area and to assess the influence of geographical, climatic and dwelling factors. methods: dust samples were collected in a transversal descriptive study from mattresses of 51 patients who went to the ... | 2011 | 21550163 |
| characterization of an immunomodulatory der p 2-fip-fve fusion protein produced in transformed rice suspension cell culture. | der p 2, a major allergen of dermatophagoides pteronyssinus mites, is one of the most clinically relevant allergens to allergic patients worldwide. fip-fve protein (fve) from the golden needle mushroom (flammulina velutipes) is an immunomodulatory protein with potential th1-skewed adjuvant properties. here, we produced and immunologically evaluated a der p 2-fve fusion protein as a potential immunotherapeutic for allergic diseases. using an inducible expression system in cultured rice suspension ... | 2011 | 21556691 |
| in-vivo allergenic potency of siriraj mite allergen vaccine (smav) comparing with standardized vaccine in mite-sensitive patients. | the prevalence of allergic diseases, particularly asthma and allergic rhinitis, has increased tremendously in thailand and worldwide. house dust mite (hdm) is the major ige sensitizer among allergic children and adults. we have developed local standardized mite allergen extracts, siriraj mite allergen vaccine (smav) from dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df) from our source materials which were highly purified (99%). | 2011 | 21560488 |
| construction of a der p2-transgenic plant for the alleviation of airway inflammation. | in clinical therapy, the amount of antigen administered to achieve oral tolerance for allergic diseases is large, and the cost is a major consideration. in this study, we used tobacco plants to develop a large-scale protein production system for allergen-specific immunotherapy, and we investigated the mechanisms of oral tolerance induced by a transgenic plant-derived antigen. we used plants (tobacco leaves) transgenic for the dermatophagoides pteronyssinus 2 (der p2) antigen to produce der p2. m ... | 2011 | 21602845 |
| allergenic dermatophagoides mites causing asthma among schoolchildren at ain-shams district, cairo, egypt. | the present study was performed on house dust samples collected from ten homes of schoolchildren suffering from asthma at ain-shams district, over a period of two years (2008 & 2009). the data revealed that the total annual density of the two allergenic mites (dermatophagoides pteronyssinus (trouessart) & dermatophagoides farinae (hughes) was 202 individual mites with 22 asthmatic children in the first year, and individual mites increased to 268 with 36 asthmatic children in the second year. the ... | 2011 | 21634241 |
| allergic conjunctivitis and conjunctival provocation tests in atopic dogs. | canine atopic dermatitis (cad) is a very common disease, but little is known about eye involvement. the conjunctival provocation test (cpt) is used in human to study the ocular response to allergenic stimuli and to evaluate anti-allergic therapy. to our knowledge it has not been used in dogs. | 2011 | 21733066 |
| specific tear ige in patients with moderate-to-severe autumnal allergic conjunctivitis. | background: recently, a new commercial test for specific ige based on immunochromatography (immfast check j1-«) has been developed. we previously reported on the use of this kit for tear fluid samples during spring. in this study, we compared the level of specific ige in tear fluid among patients with seasonal allergic conjunctivitis during spring or autumn and control subjects. methods: a nonrandomized, cross-sectional study was conducted in patients with moderate-to-severe allergic conjunctivi ... | 2011 | 21829033 |
| repeated high-dose inhalation allergen challenge in asthma. | inhalation allergen challenge in humans is used to investigate lung pathophysiology and responses to novel therapies. however, the single high-dose allergen challenges that are commonly performed do not mimic repeated symptomatic environmental allergen exposure. | 2011 | 21679350 |
| efficacy of sublingual immunotherapy with house dust mite extract in polyallergen sensitized patients with allergic rhinitis. | it is suggested that polysensitized patients might not benefit from specific allergic rhinitis immunotherapy as much as monosensitized patients, although further research on this subject is needed. | 2011 | 21704889 |
| the pattern of sensitisation to inhalant allergens in omani patients with asthma, allergic rhinitis and rhinoconjunctivitis. | identification of relevant allergens that are prevalent in each environment which may have diagnostic and therapeutic implications in allergic diseases. this study aimed to identify the pattern of sensitisation to inhalant allergens in omani patients with asthma, allergic rhinitis and rhinoconjunctivitis. | 2008 | 21748078 |
| effects of helicobacter pylori, geohelminth infection and selected commensal bacteria on the risk of allergic disease and sensitization in 3-year-old ethiopian children. | background epidemiological studies have suggested that gastro-intestinal infections including helicobacter pylori, intestinal microflora (commensal bacteria) and geohelminths may influence the risk of asthma and allergy but data from early life are lacking. objective we aimed to determine the independent effects of these infections on allergic disease symptoms and sensitization in an ethiopian birth cohort. methods in 2008/09, 878 children (87% of the 1006 original singletons in a population-bas ... | 2011 | 21831135 |
| passive airborne dust sampling to assess mite antigen exposure in farming environments. | the aim of this study was to estimate mite antigen exposure in farming environments by passive sampling of airborne dust. antigen concentrations were measured with enzyme immunoassays specific for three storage mites (sm): acarus siro, lepidoglyphus destructor, tyrophagus putrescentiae and the house dust mite (hdm) dermatophagoides pteronyssinus. dust samples were collected with electrostatic dust fall collectors (edcs) in three different areas of cattle farms. edcs were placed in cow stables (w ... | 2011 | 21842065 |
| acaricidal activities of apiol and its derivatives from petroselinum sativum seeds against dermatophagoides pteronyssinus, dermatophagoides farinae, and tyrophagus putrescentiae. | the acaricidal effects of an active constituent derived from petroselinum sativum seeds and its derivatives were determined using impregnated fabric disk bioassay against dermatophagoides farinae , dermatophagoides pteronyssinus , and tyrophagus putrescentiae and compared with that of synthetic acaricide. the acaricidal constituent of p. sativum was isolated by various chromatographic techniques and identified as apiol. on the basis of ld(50) values against d. farinae and d. pteronyssinus, apiol ... | 2011 | 21688847 |
| Seasonal Dynamics of House Dust Mites in Dust Samples Collected From Sleeping Places in North-Western Poland. | The most common families of mites found in house dust are Pyroglyphidae, Glycyphagidae and Acaridae; all are a source of many antigens responsible for allergic diseases. The aim of this study was to examine the seasonal dynamics of allergenic mite populations in dust samples collected from sleeping places in apartments in north-western Poland. The mites were isolated from the dust using a saturated saline floating method. In 132 dust samples we determined: Dermatophagoides farinae, Dermatophagoi ... | 2011 | 21824362 |
| nerve growth factor derived from bronchial epithelium after chronic mite antigen exposure contributes to airway hyperresponsiveness by inducing hyperinnervation, and is inhibited by in vivo sirna. | background: bronchial asthma is a chronic allergic airway inflammatory disease. neurotrophins, including nerve growth factor (ngf), play an important role in the pathogenesis of asthma. however, the effects of ngf derived from epithelium on airway hyperresponsiveness (ahr) after antigen sensitization/exposure remain uncertain. objective: in this study, we examined the role of ngf on ahr after chronic antigen exposure and the effect of inhibiting ngf by in vivo sirna on ahr exacerbation. methods: ... | 2011 | 22168511 |
| [skin prick tests with standardized extracts of mites of different precedence in patients with asthma and allergic rhinitis]. | background: house dust mites are important airborne allergens worldwide, particularly in the tropical environment. objective: to assess sensitization of allergic patients to different mite species in the cuban city of camaguey; as well as to compare standardized allergen extracts from different sources by skin prick tests. patients and method: skin prick tests were performed in 60 patients with asthma and/or allergic rhinitis and in 60 non-allergic volunteers, ran ... | 2010 | 21944487 |
| [Aeroallergen spectrum of patients with allergic rhinitis in Enshi area]. | To investigate aeroallergen spectrum of patients with allergic rhinitis in Enshi area and analyze its related factors. | 2011 | 22032124 |
| passive smoking and respiratory allergies in adolescents. | the aim of our study was to investigate impact of active and passive smoking on total and specific serum ige levels and on incidence of developing allergic diseases (i.e. asthma, rhinitis) in a group of croatian adolescents. | 2011 | 21845810 |
| skin prick test reactivity to aeroallergens in jordanian allergic rhinitis patients. | identification of the most common aeroallergens to which patients are sensitized in a specific area is important in the diagnosis and treatment of allergic rhinitis. the aim of this cross-sectional study was to investigate the pattern of skin prick test reactivity to various aeroallergens among allergic rhinitis patients attending outpatient clinics in amman, jordan. skin prick test with 18 standardized allergen extracts was performed on 538 patients. most allergic rhinitis patients in the study ... | 2011 | 21972485 |
| skin prick testing to aeroallergen extracts: what is the optimal panel in children and adolescents in turkey? | background/objective: the skin prick test (spt) is the standard tool for the diagnosis of ige-mediated allergic diseases. the number and spectrum of allergen extracts to be applied for the diagnosis of sensitized patients in order to achieve the most cost-effective and informative results are critical from both economic and scientific viewpoints. methods: in order to determine the minimum test battery panel necessary to cover at least 95% of the cases of spt sensitization in children and adolesc ... | 2011 | 22123205 |
| histamine and allergen skin reactivity in the elderly population: results from the korean longitudinal study on health and aging. | the allergen skin prick test is the most representative diagnostic test for atopy, but its use has been limited because of the belief that skin reactivity decreases with aging. | 2011 | 21962095 |
| sensitization to aeroallergens in korean children: a population-based study in 2010. | we performed this study to assess the prevalence of sensitization to aeroallergens and to analyze the difference between prevalence rates according to children's ages and residential areas. in this nationwide cross-sectional study, first grade students of 45 elementary schools and 40 middle schools were randomly selected, and skin prick tests were performed for 18 inhalant allergens between october and november 2010. of 7,829 analyzed subjects, 3,753 (47.9%) were sensitized to at least one aeroa ... | 2011 | 21935271 |
| evaluation of therapeutic sublingual vaccines in a murine model of chronic house dust mite allergic airway inflammation. | second generation therapeutic vaccines based upon recombinant allergens or natural extracts, potentially formulated in vector systems or adjuvants, are being developed. to this aim, preclinical studies in relevant animal models are needed to select proper allergens, formulations and administration schemes. | 2011 | 22092967 |
| exhaled nitric oxide is associated with allergic inflammation in children. | exhaled nitric oxide (eno) has been proposed as a noninvasive marker of airway inflammation in asthma. in asthmatic patients, exhaled no levels have been shown to relate with other markers of eosinophilic recruitment, which are detected in blood, sputum, bronchoalveolar lavage fluid and bronchial biopsy samples. the purpose of this study was to assess the possible relationship between eno and allergic inflammation or sensitization in childhood asthma and allergic rhinitis. subjects consisted of ... | 2011 | 22022176 |
| In planta expression of a mature Der p 1 allergen isolated from an Italian strain of Dermatophagoides pteronyssinus. | European (Dermatophagoides pteronyssinus) and American (Dermatophagoides farinae) house dust mite species are considered the most common causes of asthma and allergic symptoms worldwide. Der p 1 protein, one of the main allergens of D. pteronyssinus, is found in high concentration in mites faecal pellets, which can became easily airborne and, when inhaled, can cause perennial rhinitis and bronchial asthma. Here we report the isolation of the Der p 1 gene from an Italian strain of D. pteronyssinu ... | 2011 | 21904913 |
| Modulation of the Humoral Response to Dermatophagoides pteronyssinus Allergens in BALB/c Mice by Extract Modification and Adjuvant Use. | Background: Currently, several strategies are being used in order to improve the safety and efficacy of allergen-specific immunotherapy; these strategies include the use of modified hypoallergenic extracts as well as different adjuvants with immunomodulatory properties in combination with native or modified extracts. The objectives of this study were to investigate the humoral response generated in mice to modified Dermatophagoides pteronyssinus extracts in the presence or absence of two differe ... | 2011 | 22123212 |
| maintenance dosing for sublingual immunotherapy by prominent european allergen manufacturers expressed in bioequivalent allergy units. | sublingual immunotherapy (slit) has become established in europe, and its efficacy is being evaluated in the united states. the doses used for slit in europe today are difficult to evaluate, because each manufacturer expresses the potency of its extracts differently. | 2011 | 22018618 |
| association of house dust mite-specific ige with asthma control, medications and household pets. | evidence is conflicting regarding the effectiveness of creating a low-allergen environment or reducing allergen exposure to control asthma exacerbations. | 2011 | 22053311 |
| Mother to child transfer of IgG and IgA antibodies against Dermatophagoides pteronyssinus. | There is strong evidence from animal models that placental and/or breast milk-mediated transfer of maternal allergen-specific IgG prevents allergic immune responses in the progeny. Both human and animal data also point to IgA as having an important regulatory role. In contrast, little is known about maternal transfer of IgG and IgA specific for respiratory allergens in humans. Dermatophagoides pteronyssinus (Der p) is an indoor allergen that is a major cause of asthma worldwide. We analysed mate ... | 2011 | 21883350 |
| [aeroallergen spectrum of patients with child allergic rhinitis in changsha area of china]. | to investigate aeroallergen spectrum and allergy positive rates of patients with child allergic rhinitis and analyze its related factors. | 2011 | 22070068 |
| Modelling and Bioinformatics Analysis of the Dimeric Structure of House Dust Mite Allergens from Families 5 and 21: Der f 5 Could Dimerize as Der p 5. | Allergy represents an increasing thread to public health in both developed and emerging countries and the dust mites Dermatophagoides pteronyssinus (Der p), Blomia tropicalis (Blo t), Dermatophagoides farinae (Der f), Lepidoglyphus destructor (Lep d) and Suidasia medanensis (Sui m) strongly contribute to this problem. Their allergens are classified in several families among which families 5 and 21 which are the subject of this work. Indeed, their biological function as well as the mechanism or e ... | 2012 | 22208271 |
| the hammock: a reservoir of allergens. | asthma affects approximately 10% of the world's population. sensitization to allergens is an important risk factor, and exposure to allergens is associated with disease severity. | 2011 | 21876974 |