| age dependency of tissue resistance to trichinella spiralis in chinese hamsters. | | 1967 | 6026861 |
| trichinella spiralis in india. ii. characteristics of a strain isolated from a civet cat in calcutta. | | 1967 | 6047759 |
| [intradermal test with allergens for trichinella spiralis]. | | 1967 | 6056125 |
| delayed hypersensitivity to trichinella spiralis. i. transfer of delayed hypersensitivity by lymph node cells. | | 1967 | 6060966 |
| delayed hypersensitivity to trichinella spiralis. ii. antibody response in recipients after transfer of delayed hypersensitivity. | | 1967 | 6060967 |
| the life cycle of trichinella spiralis. i. the intestinal phase of development. | | 1967 | 6064063 |
| the life cycle of trichinella spiralis. ii. the muscle phase of development and its possible evolution. | | 1967 | 6064064 |
| distribution of trichinella spiralis in the small intestine of young mice. | | 1967 | 6067114 |
| trichinella spiralis in dogs and cats. | | 1967 | 6067418 |
| trichinella spiralis in india. i. its history in india, rediscovery in calcutta, and the ecology of its maintenance in nature. | | 1967 | 6069061 |
| immunoelectroadsorption studies on trichinella spiralis. | | 1967 | 6078614 |
| [experimental trichinosis. i. survival and reproduction of the various stages of trichinella spiralis in the peritoneal cavity of mice]. | | 1967 | 6083110 |
| osteolysis in trichinella spiralis infected vs noninfected rats. | 1. based upon the present inability to describe the mechanism by which calcification takes place, and the unexplored metabolism of calcium during trichinosis, studies of rat bone involvement were undertaken. 2. significant (p less than 0.05) differences were found in experimental (trichinella spiralis infected) rat femurs vs those of noninfected rats. 3. analysis by atomic absorption spectroscopy, dry femur weight, and tensile stress resistance indicated an overall reduction in calcium content. ... | 1982 | 6124348 |
| studies on the inhibition of rapid expulsion of trichinella spiralis in rats. | a variety of inhibitors was examined for their ability to interfere with the expression of rapid expulsion (re) of challenge trichinella spiralis infections in rats. inhibitors of immediate hypersensitivity, prostaglandin release, peristalsis, or complement function, did not impair re when administered to immune rats. induction of intestinal anaphylaxis against t. spiralis or ovalbumin by passive serum transfer to intestinally primed rats (prior infection with heligmosomoides polygyrus) or admin ... | 1982 | 6125479 |
| immune response to stage-specific surface antigens of the parasitic nematode trichinella spiralis. | rats were infected with the nematode trichinella spiralis and the primary serum antibody response to antigenic surface proteins of infective larvae, intestinal worms, and newborn larvae was studies. 1 wk after infection, the sera contained antibodies to surface antigens of both infective larvae and intestinal worms. these early sera, however, failed to react with newborn larvae surface antigens. in addition, adsorption of sera with living intestinal worms or infective larvae removed antibodies t ... | 1981 | 6166724 |
| oxatomide protects against degranulation of rat peritoneal mast cells during in vitro challenge with antigen or compound 48/80. ultrastructural aspects. | the ultrastructure of isolated rat peritoneal mast cells was evaluated after in vitro degranulation and treatment with oxatomide, a new anti-allergic compound. in a first series of experiments, mast cells of rats infected with trichinella spiralis larvae were incubated with trichinella larvae somatic antigen to produce histamine release. the release was visualized in the electron microscope by exocytosis of the peripheral amine-containing granules, which resulted from fusion between several peri ... | 1981 | 6167155 |
| antigen-mediated release of eosinophil growth stimulating factor from trichinella spiralis sensitized spleen cells: a comparison of t. spiralis stage-specific antigen preparations. | when non-adherent, trichinella spiralis-sensitized mouse spleen cells were challenged in vitro with t. spiralis antigens, an eosinophil growth factor (eo-gsf) was released into the culture medium. this factor was assayed by its ability to initiate eosinophil production in liquid cultures of syngeneic, non-adherent marrow cells obtained from unsensitized mice. extracts of each parasite stage as well as excretory-secretory (es) products of adult and muscle larva stages were compared for their abil ... | 1982 | 6175563 |
| the occurrence of antibodies to hidden and exposed determinants of surface antigens of trichinella spiralis. | mice were infected per os with trichinella spiralis and their lymphocytes were removed and fused with mouse myeloma cell line p3 x 63ag8653p3 for the selection of monoclonal antibodies to biochemically defined, stage-specific surface antigens of 3 parasite developmental stages: muscle larvae, adults and newborn larvae. two separate antibodies against a defined single surface antigen of each stage were isolated. in each separate case the pair of monoclonal antibodies precipitated the same compone ... | 1984 | 6201803 |
| schistosoma mansoni, s. haematobium, and s. japonicum: identification of genus- and species-specific antigenic egg glycoproteins. | immunoreactive egg glycoproteins of schistosoma mansoni, s. haematobium, and s. japonicum which are genus- and species-specific, or react with sera of patients infected with other parasites, have been identified. egg proteins were labeled with iodine-125, and the concanavalin a-binding glycoproteins were immunoprecipitated with sera of patients infected with one of four species of schistosoma or trichinella spiralis, taenia solium, echinococcus granulosus, entamoeba histolytica, or wuchereria ba ... | 1984 | 6209162 |
| induction of ia antigen in rat epidermal cells and gut epithelium by immunological stimuli. | the expression of ia antigen in rat keratinocytes and gut epithelium was found to be inducible by a variety of immunological stimuli. graft-vs.-host disease (gvhd) was accompanied by the appearance of ia antigen in both sites, whereas local immunological stimuli, such as a contact-sensitizing agent applied to the skin and trichinella spiralis infection of the gut, caused the expression of ia antigen confined to the sites of contact of these stimuli with the tissues involved. both t helper and t ... | 1982 | 6217272 |
| strongyloides ratti and trichinella spiralis: net charge of epicuticle. | the intact epicuticles of strongyloides ratti stage-3 larvae and trichinella spiralis stage-1 larvae were found to have a surface net negative charge. ultrastructural studies on s. ratti using cationized ferritin and ruthenium red showed the negative charge to be dense and uniformly distributed over the epicuticular surface. staining with acetic acid-ferric oxide hydrosol occurred at ph 1.65 and suggests that amino acid carboxyl groups were not responsible for the negative charge property. alcia ... | 1983 | 6221941 |
| some properties of the surface of nematode larvae. | the nature of complement binding to the surface to infective larvae of trichinella spiralis and nippostrongylus brasiliensis differs. when worms were incubated in serum from uninfected hosts, washed and incubated in fluorescent reagent the whole surface of t. spiralis fluoresced but in n. brasiliensis fluorescence was confined to the anterior end and some internal organs. the outer structure of the cuticle of the t. spiralis larvae was shown not to contain atp-ase, thus differing from many cell ... | 1983 | 6230386 |
| the killing of newborn larvae of trichinella spiralis by eosinophil peroxidase in vitro. | helminth infections in mammals are characterized by a high level of eosinophils in parasitized tissues and blood, and it has recently been suggested that these cells have a direct parasiticidal effect. newborn larvae of trichinella spiralis can be killed within 20 min by incubation at room temperature in a cell-free system, including purified human eosinophil peroxidase (epo), h2o2 and chloride at ph 5.5. killing was measured by microscopic observation of the larvae. the larvicidal effect was de ... | 1981 | 6273182 |
| myeloperoxidase is more efficient than eosinophil peroxidase in the in vitro killing of newborn larvae of trichinella spiralis. | myeloperoxidase (mpo) and eosinophil peroxidase (epo) catalyse the formation of hypochlorite (ocl-) from chloride ions (ocl-) and hydrogen peroxide (h2o2). ocl- proved to be highly toxic for trichinella spiralis newborn larvae (nbl) in in vitro assays. using purified human mpo and epo it was found that even at neutral ph both enzymes under appropriate conditions are able to kill nbl. the rate at which ocl- is produced is much lower in the epo- than in the mpo-mediated reaction. this difference i ... | 1984 | 6321330 |
| delayed expulsion of adult trichinella spiralis by mast cell-deficient w/wv mice. | mast cell-deficient w/wv mice and their mast cell-sufficient littermates were given infections of trichinella spiralis. w/wv mice were slower than their littermates to expel adult t. spiralis. repair of the mast cell deficiency of w/wv mice by bone marrow grafting was accompanied by accelerated expulsion of t. spiralis. | 1983 | 6345400 |
| [immunofluorescent reaction of antibodies against antigens of the intestinal epithelium of schistosoma mansoni. i--in experimentally infested mice]. | the present study was carried out to test with the immunofluorescent antibody test the presence of antibodies directed against the gut epithelium of adult schistosomes in experimental schistosomiasis in mice. paraffin sections of adult female worms fixed in rossman's fixative were used and antibodies of the different classes and subclasses could be studied with monospecific conjugates. igm were the predominant antibodies: they appeared at day 12 after infection, reached a peak value by the 2nd m ... | 1983 | 6347008 |
| enzyme immunoassay for swine trichinellosis using antigens purified by immunoaffinity chromatography. | various preparations of crude and a purified preparation of trichinella spiralis antigens were compared in a rapid, micro-enzyme immunoassay (eia) for detecting trichinellosis in swine. the crude antigen preparations (xm-300 or s3 fraction) were lipid-free, cell-free fractions of muscle larvae, and the purified antigen was prepared by immunoaffinity chromatography of the soluble fraction of stichocyte secretory granules from rat muscle larvae. the antigens were tested against normal and immune s ... | 1983 | 6359908 |
| evaluation of micro-elisa for schistosomiasis japonica using crude egg antigen. | procedures of micro-elisa for detecting antibody of schistosoma japonicum infection were improved by using crude egg antigen, peroxidase-labeled antibody and o-phenylenediamine on a micro-elisa plate (m129a, dynatech). reactions were performed with 0.1 ml of reagents in 0.3 ml wells at each step and 0.3 ml of substrate was placed at the final procedure. the endpoint of reaction was defined as the upper limit of 99% critical range of absorbance in negative sera at 1:40 dilution which was approxim ... | 1983 | 6363767 |
| protection against trichinella spiralis induced by a monoclonal antibody that promotes killing of newborn larvae by granulocytes. | mouse monoclonal antibodies directed against biochemically defined surface antigens of trichinella spiralis were selected and tested for their ability to destroy parasites in vivo and in vitro. one of these (nim-m5; igg1), which recognised a surface component of approximately 64 k molecular weight in newborn larvae (nbl), bound to a surface component of this stage (as shown by fluorescence), and mediated the adherence of rodent eosinophil leucocytes to the surface of living nbl. following cell a ... | 1984 | 6377201 |
| in vivo and in vitro responses to sheep erythrocytes by lymph node cells from mice with trichinellosis. | mice infected with trichinella spiralis for 20 days have decreased numbers of plaque forming cells (pfc) in the draining lymph nodes following subcutaneous immunization with sheep erythrocytes. however, when immunized in vitro, lymph node cells from infected mice generate more pfc than normal controls. splenectomy has no effect on suppression observed in vivo. there is a large increase in the proportion and numbers of b cells in the lymph nodes of infected mice, and these cells may account for t ... | 1984 | 6380831 |
| immunomodulation by nematodes: a review. | there is current evidence that infections with trichinella spiralis, ascaris suum, nippostrongylus brasiliensis, nematospiroides dubius (syn. heligmosomoides polygyrus) and diverse filariae affect the immune responsiveness of their hosts. t. spiralis, or its extracts, can depress or enhance the heterologous humoral or cell-mediated immunities, and affect macrophage activity or the response to other invaders. these effects are induced by products of the migratory and early muscle larvae and appea ... | 1984 | 6382784 |
| comparison of monoclonal antibody-based competitive and indirect enzyme-linked immunosorbent assays for the diagnosis of swine trichinosis. | a competitive enzyme-linked immunosorbent assay (elisa) for the detection of swine trichinosis has been developed using a biotinylated monoclonal antibody and an avidin-enzyme conjugate. the assay is based on competitive binding between swine serum antibodies and a monoclonal antibody specific for an antigenic determinant present on proteins from trichinella spiralis excretory-secretory products with molecular weights of 45,000, 49,000, and 53,000. the competitive elisa reliably detected pigs in ... | 1984 | 6385467 |
| expulsion of trichinella spiralis from the intestine of w/wv mice reconstituted with haematopoietic and lymphopoietic cells and origin of mucosal mast cells. | the involvement of haematopoietic and lymphopoietic cells in the expulsion of trichinella spiralis from the intestine of mice, and the origin of the subepithelial mast cells (smcs) and the globule leucocytes (gls) were determined using smc- and gl-deficient wbb6f1-w/wv and wcb6f1-s1/s1d mice. on day 12 after infection with t. spiralis, smcs and gls appeared in w/wv mice injected with spleen and bone marrow cells from normal +/+ mice, but not in w/wv mice injected with thymus, mesenteric lymph no ... | 1984 | 6386673 |
| distribution, prevalence and intensity of trichinella spiralis infection in furbearing mammals of pennsylvania. | tongues from 1,170 raccoons, 384 opossums, 201 muskrats, 168 foxes (red and grey), 51 skunks, and 17 mink from pennsylvania were examined for trichinella spiralis larvae by peptic digestion. the overall prevalence of t. spiralis infection was 3.2%, varying in the carnivores from 2.6% in raccoons to 15.1% in red foxes; no muskrats were infected. prevalence varied regionally, being higher west of the susquehanna river, where, in the ridge and valley province, it reached 9%. the larval densities va ... | 1984 | 6387078 |
| genetic control of immunity to trichinella spiralis: influence of h-2-linked genes on immunity to the intestinal phase of infection. | | 1983 | 6401687 |
| trichinella spiralis: role of non-h-2 genes in resistance to primary infection in mice. | two strains of mice which share identical h-2 genes but differ in their genetic backgrounds were compared for their ability to resist infection with trichinella spiralis. the two strains of mice, c3heb/fej and akr/j, share the h-2k haplotype which is associated with susceptibility to primary infection with t. spiralis in h-2 congenic strains of mice. akr/j mice, infected with 150 infective muscle larvae, harbored significantly fewer muscle larvae 30 days postinfection than did mice of the strain ... | 1983 | 6403370 |
| a gene mapping between the s and d regions of the h-2 complex influences resistance to trichinella spiralis infections of mice. | the strains b10.s(7r), b10.s(23r) and b10.s(24r), all thought to be genetically identical, differ in levels of susceptibility to infection with trichinella spiralis. in a series of nine independent experiments, b10.s(7r) was shown to be more susceptible than the other two strains. in another series of seven experiments, the strain b10.a(18r) was shown to be more susceptible to infection with t. spiralis than the strains b10.s(21r) or b10.bar-5, all of which were thought to share common h-2 allel ... | 1983 | 6417245 |
| genetic control of immunity to trichinella spiralis infections of mice. hypothesis to explain the role of h-2 genes in primary and challenge infections. | h-2 congenic strains of mice were compared for their ability to expel t. spiralis infections from the small intestine and for their ability to limit the reproduction of adult female worms. b10.m mice (h-2f) expelled both primary and challenge infections more quickly than did the strains b10.q(h-2q) and b10.br(h-2k). during a primary infection, expulsion of worms from b10.m mice began before day 9 post-infection and worm counts differed significantly (p less than .05 student's t-test) from counts ... | 1984 | 6423524 |
| [possibilities of survival of various parasites in meat and meat products]. | the consumption of meat and meat products may result in parasitic infection in man. trichinella spiralis infection can be very dangerous, but this parasite is now rarely encountered in the netherlands; the same applies to taenia solium. taenia saginata is regularly detected but is not particularly harmful to the consumer. the pathogenicity of sarcocystis bovihominis and sarcocystis suihominis for humans remains uncertain. toxoplasma gondii may give rise to severe problems, particularly congenita ... | 1984 | 6427970 |
| a single gene determines rapid expulsion of trichinella spiralis in mice. | in rats and some inbred mouse strains, one immune response, rapid expulsion, confers up to 95% protection against a challenge infection with trichinella spiralis. strain analysis in mice has shown that only three inbred strains, all originating from swiss-line mice at the national institutes of health, bethesda, md., express rapid expulsion. crosses between responder strain mice (nfr/n) and nonresponders (c3h/hej or b10 x br) have indicated that rapid expulsion is dominant and autosomal (bell et ... | 1984 | 6429049 |
| effect of size of trichinella spiralis (nematode) infections on glucose and ion transport in the rat intestine. | an in vivo perfusion technique, using 3 intestinal loops representing the anterior, mid and posterior regions of the rat small intestine, was used to determine intestinal glucose uptake 5 days after infection with trichinella spiralis. at high levels of infection (3,000 and 6,000 larvae/rat) net glucose absorption by the intestinal mucosa was significantly impaired in all regions of the small intestine when compared to uninfected controls. at low levels of infection (50 larvae/rat) glucose uptak ... | 1984 | 6438293 |
| preconditioning of trichinella spiralis nativa larvae in musculature to low temperatures. | preconditioning of a trichinella spiralis nativa isolate in ferret and fox musculature was carried out by freezing at -15 degrees c from 0 to 322 days prior to low temperature refrigeration at -32 degrees c. a limited number of preconditioned samples of infected fox musculature was also refrigerated at -45 degrees c. preconditioned larvae were appreciably more resistant than those subjected to the low temperatures directly. under the conditions of this investigation, the longer the period of pre ... | 1984 | 6441341 |
| basophils and eosinophils in three strains of rats and in athymic (nude) rats following infection with the nematodes nippostrongylus brasiliensis or trichinella spiralis. | a previous report showed that infection with the nematode nippostrongylus brasiliensis stimulates a basophilia as well as an eosinophilia in the blood of august rats. the present study shows that blood levels of basophils and eosinophils were increased in two other rat strains, one inbred and one outbred, after infection with n. brasiliensis, and infection of two inbred rat strains with trichinella spiralis also stimulated a basophilia as well as an eosinophilia. no increase occurred in basophil ... | 1980 | 6449471 |
| the influence of some anthelmintics on the bioenergetic metabolism of trichinella spiralis and trichinella pseudospiralis. | | 1984 | 6466367 |
| oxfendazole: regimen-dependent expression of drug efficacy against trichinella spiralis. | | 1984 | 6469447 |
| development of the male genitalia of trichinella spiralis during the enteral phase of infection in the mouse: an sem study. | | 1984 | 6470896 |
| rapid expulsion of trichinella spiralis in suckling rats. | orally administered trichinella spiralis muscle larvae were rapidly expelled by rat pups suckling an immune dam. the immunity was delivered in the milk; substantial resistance was conferred on normal rat pups suckled for only 24 hours by a trichinella-immune foster mother. the pups were protected by oral or systemic administration of specific serum antibodies. when infused into a normal lactating dam, these antibodies accumulated in the serum of her suckling pups. | 1984 | 6474191 |
| toxicity of bis(tri-n-butyltin)oxide in the rat. ii. suppression of thymus-dependent immune responses and of parameters of nonspecific resistance after short-term exposure. | to evaluate the functional significance of bis(tri-n-butyltin)oxide (tbto)-induced thymus atrophy, lymphocyte depletion in spleen and lymph nodes, lymphopenia, and increased serum igm and decreased igg concentrations, in vivo and in vitro function studies were performed for specific and nonspecific resistance. weaned male rats were fed diets containing 0, 20, or 80 mg tbto/kg for at least 6 weeks. regarding the thymus-dependent immunity, delayed-type hypersensitivity reactions to ovalbumin as we ... | 1984 | 6474470 |
| tissue and blood eosinophilia in mast cell-deficient sl/sld mice infected with trichinella spiralis. | | 1984 | 6482120 |
| immunodiagnosis of giardiasis by elisa and studies on cross-reactivity between the anti-giardia lamblia antibodies and some heterologous parasitic antigens and fractions. | specific antibodies against giardia lamblia in 92 sera from asymptomatic carriers were investigated by elisa. using cyst antigen, antibodies were detected in 91 of the sera, by means of alkaline phosphatase-labelled antispecies immunoglobulin. the e/405nm values of the positive sera ranged between 0.350 and 1.700, whereas in the 78 control sera they were 0.270 +/- 0.220. cross-reactivity between the anti-g. lamblia antibodies and 18 heterologous parasitic antigens and fractions was also studied. ... | 1984 | 6486935 |
| stage-specific antigens of trichinella spiralis. | infective larvae, adults and newborn larvae of trichinella spiralis were surface labelled with radioactive iodine, and the surface material was solubilized in the mild detergent sodium deoxycholate. the radio-isotope labelled products were stage-specific glycoproteins that were few in number (2-4 components) and antigenic in infected mice and rats. antibodies synthesized in infected animals against these biochemically defined surface antigens may or may not interact with the surface of the livin ... | 1984 | 6493807 |
| evasion of the immune response: survival within low responder individuals of the host population. | it is proposed that, for many species of parasites, evasion of the host immune response may be achieved passively through enhanced survival within host individuals that have a genetically determined low responsiveness to infection. evasion by this means may contribute significantly to continued transmission of infection in man and domestic animals and influence the severity of pathology. low responsiveness plays an important role in determining over-dispersion of parasites within host population ... | 1984 | 6493808 |
| h-2-controlled, dose-dependent suppression of the response that expels adult trichinella spiralis from the small intestine of mice. | h-2 congenic strains of mice expressing the h-2k, h-2q or h-2f haplotype were tested for their ability to expel trichinella spiralis from the gut following infection with either 100, 150, 200, 400, 500, or 600 l1 infective larvae. h-2q and h-2f mice expelled worms more quickly than h-2k mice when 100-200 l1 larvae were given, but this h-2-controlled effect was much reduced when mice received 400 l1 larvae, and completely eliminated when 500 or 600 l1 larvae were given. the observed dose-dependen ... | 1984 | 6500629 |
| immune rejection of trichinella spiralis infective larvae in the guinea pig. | | 1984 | 6502363 |
| mucosal mast cells are functionally active during spontaneous expulsion of intestinal nematode infections in rat. | infestation of the gastrointestinal tract by parasitic nematodes is invariably associated with mucosal mastocytosis, which is a thymus-dependent phenomenon in parasitized rats, and is adoptively transferable with a t cell-enriched population of thoracic duct lymphocytes. when derived by in vitro culture, mucosal mast cells (mmc) arise from a bone marrow precursor after stimulation by t cell-derived factors. in rats infected with the nematode trichinella spiralis, mucosal mastocytosis is temporal ... | 1984 | 6504156 |
| altered host resistance to trichinella spiralis infection following subchronic exposure to diethylstilbestrol. | the effects of subchronic exposure to diethylstilbestrol on the host response to infection with trichinella spiralis were examined in adult b6c3f1 mice. expulsion of adult trichinella from the small bowel, intestinal inflammation and delayed hypersensitivity responses to trichinella antigens in artificially sensitized mice were investigated. administration of 8 micrograms/g of diethylstilbestrol for five consecutive days beginning on days -5,0, +3 or +8 of infection inhibited adult worm expulsio ... | 1984 | 6511144 |
| tissue eosinophil numbers and phospholipase b activity in mice infected with trichinella spiralis. | | 1984 | 6511177 |
| the intestinal mast cell response to trichinella spiralis infection in mast cell-deficient w/wv mice. | the intestinal mast cell response and lymphoblast activity, as measured by the incorporation of 3h-thymidine into mesenteric lymph node cells (mln) of wbb6f1-w/wv(w/wv) mice, their normal congenic littermates (+/+) and c57bl/6j mice, were compared after infection with trichinella spiralis. marked and similar blast cell activity and an increase in number of cells were observed in the mln of infected w/wv and c57bl/6j mice 7 and 15 days p.i. in contrast to c57bl/6j mice, primary t. spiralis intest ... | 1984 | 6512640 |
| running responses of trichinella spiralis-infected cd-1 mice. | male cd-1 mice inoculated by gavage with 400 larvae of trichinella spiralis exhibited an increased latency of the wall-seeking response as well as a decline in running speed and the distance travelled to the first stop when compared with normal controls or animals inoculated with only 100 larvae. doses of 200 larvae of this parasite affected running speed and distance travelled but not the advent of the running response. such changes in behaviour may reduce the competitive fitness of the host an ... | 1984 | 6514382 |
| [first finding of trichinella spiralis in the diaphragm of a cadaver in zacatecas. preliminary note]. | | 1984 | 6515468 |
| a freeze-fracture study of the surface of the infective-stage larva of the nematode trichinella. | the surface layers of the cuticle of the infective, first-stage larva of the nematodes trichinella spiralis and t. spiralis var. pseudospiralis have been studied by means of the freeze-fracturing technique. no obvious differences between the two nematodes were found. a double-layered structure covers the cuticle. its outermost layer consists of particles embedded in an amorphous matrix; its inner layer is composed of a sheet of fine filaments which may be composed of globular subunits. this uniq ... | 1984 | 6515646 |
| the effect of the intestinal phase of trichinella spiralis on the open-field behavior of mice. | the study assessed the impact of the adult phase of trichinella spiralis on the open-field behavior of mice. thiabendazole treatment was used to chemosterilize adult worms in vivo. untreated mice exhibited a marked decline in their ambulatory and exploratory activities (from day 14 post-infection until the end of the experiment). in contrast, infected mice treated with thiabendazole did not exhibit any behavioral alterations. this suggested that the adult phase of t. spiralis did not contribute ... | 1984 | 6527189 |
| long term duration of the rapid rejection response in rats infected with trichinella spiralis. | | 1984 | 6527193 |
| the site of ecdysis of the l1 larva of trichinella spiralis. | | 1984 | 6527198 |
| interbreeding and fecundity of a single pair of two strains of trichinella spiralis in mice. | | 1984 | 6527466 |
| a comparison between the efficacy of thiabendazole and febantel on experimental trichinellosis in rats. | febantel at a dose level of 5 mg kg-1 had a lethal efficacy of 54.1% against the adult worm and 69.5% against the migrating larvae of trichinella spiralis in rats. when administered at 15 mg kg-1, it demonstrated lethal effects of 94.4, 92.5 and 74.7% against adult, young and encysted larvae, respectively. infected rats treated with 300 mg kg-1 died. thiabendazole had efficiencies of 96.4 and 83.3% against intestinal and migrating early muscle phases, respectively, and was 42% effective against ... | 1984 | 6538721 |
| the cellular ige response of rodents to infection with nippostrongylus brasiliensis, trichinella spiralis and schistosoma mansoni. | the ige response at the cellular level to helminthic infection was studied in balb/c mice inoculated with the infective larvae of the nematodes nippostrongylus brasiliensis (nb) or trichinella spiralis (ts) or with the cercariae of the trematode schistosoma mansoni (sm). changes in mesenteric lymph node (mln) cell number, cell surface(s) igd, igm, ige and thy-1.2 and intracytoplasmic (c) ige were recorded. in addition, a comparable study was conducted in rats infected with nb. at 11 days after i ... | 1984 | 6540929 |
| immunization of swine against trichinella spiralis. | swine were immunized with partially purified stichosome antigens derived from trichinella spiralis muscle larvae. in 3 trials, 500 to 600 micrograms of the solubilized particle component (s3) induced moderate levels of resistance to challenge inoculation; the percent reduction in larvae per gram of muscle ranged from 43 to 55. the effect of immunization with s3 on pig intestinal expulsion of adult worms was determined in one experiment and the results indicated that s3 immunization was only weak ... | 1984 | 6541840 |
| trichinella spiralis: genetics of worm expulsion in inbred and f1 mice infected with different worm doses. | the nematode trichinella spiralis is rejected from the intestine at a time that is characteristic for each inbred strain of mouse. previous work (r. g. bell et al. 1982a) had empirically identified strong, intermediate, and weak phenotypes (nfr, c3h/he, and c57b1/10 mice, respectively) in mice infected with 400 muscle larvae. it is shown that this classification applies to another eight inbred strains: swr, dba/2, dba/1, lp, bub/bn--all intermediate, and nzb/bin, c57l, a, and mus molossinus--all ... | 1984 | 6542023 |
| comparison of three methods for detection of prolonged experimental trichinellosis in pigs. | three methods were employed for the diagnosis of porcine trichinellosis. the pooled sample digestion method and trichinoscopy served as european community (ec) reference techniques, whereas the reliability of the enzyme linked immunosorbent assay (elisa) was tested by 11 laboratories of the european community and sweden. three groups of 6 piglets each were orally inoculated with 50, 150 and 1500 trichinella spiralis larvae into each animal. another group of 6 animals served as a non-infected con ... | 1984 | 6543048 |
| husbandry, parasitic and other diseases as factors in the reliability of the enzyme linked immunosorbent assay (elisa) for detection of trichinellosis in pigs. | serum samples were obtained from pigs originating from specified pathogen-free farms, large industrialised farms and small conventional farms. all animals proved to be free of trichinella spiralis by a pooled sample digestion method. careful meat inspection studies on parasitic infections other than trichinellosis, and other inflammatory reactions were recorded and used for subdivision of the animals in different groups. it was concluded that animal husbandry, parasitic infections, or inflammato ... | 1984 | 6543049 |
| myocarditis caused by trichinella spiralis. | | 1984 | 6546336 |
| protection to challenge with trichinella spiralis after primary oral infection in congenitally athymic (nude) mice. | | 1983 | 6600789 |
| the athymic nude rat. iv. immunocytochemical study to detect t-cells, and immunological and histopathological reactions against trichinella spiralis. | the anti-parasite response was investigated after oral infection of athymic nude (rnu/rnu) rats and heterozygous (+/rnu) littermates with 1000 muscle larvae of trichinella spiralis. no igm, igg and ige antibodies were detected in serum of rnu/rnu rats. expulsion of adult worms from the small intestine was prolonged (worms were nearly all expelled at days 14 and 91 in +/rnu and rnu/rnu rats respectively). the yield of muscle larvae in the carcasses of nude rats at day 91 was 33 times higher than ... | 1983 | 6601791 |
| trichinella spiralis: selective intestinal immune deviation in the rat. | in rats, infections with 100-2000 trichinella spiralis muscle larvae lead to a prompt immunity that is expressed in parasite expulsion within 14 days. rats infected with more than 2000 larvae display impaired immunity with rejection delayed by 50% (7 days) or more. suppression is selective for expulsive immunity as the antifecundity response of rats is directly proportional to dose and is expressed sooner in heavily infected subjects. suppression of intestinal expulsive immunity was suggested by ... | 1983 | 6603372 |
| cloned t cells recognize trichinella spiralis antigen in association with an ek beta ek alpha restriction element. | a method is described for the production of t-cell lines and clones specific for solubilized trichinella spiralis antigens. these t cells are antigen-specific and do not respond to challenge with a third party antigen (lysozyme). the proliferation responses of the cloned t cells are specifically inhibited by anti-i-e but not by anti-i-a subregion monoclonal reagents. the inhibition patterns obtained are consistent with cis-gene complementation in b10.k cells involving the ek beta-chain and the e ... | 1983 | 6605915 |
| demonstration of anti-muscle immunity in murine trichinellosis. | swiss mice infected with trichinella spiralis (i mice), or immunized with straight freund's complete adjuvant (a mice) or with rat muscle extract (rme) in freund's complete adjuvant (ma mice) were tested for skin reactivity against rme and compared with uninfected, unimmunized, tested controls (c mice). c, a and i mice infected for 32 days did not show any reaction; ma mice exhibited significant enlargement of the foot-pads at 24 h of the skin inoculation when tested 7 days after immunization an ... | 1983 | 6612988 |
| the effect of diethylstilbestrol as measured by host resistance and tumor susceptibility assays in mice. | as part of a program to develop and validate methodology to measure chemically induced immunotoxicity, the effect of des on resistance of adult b6c3f1 female mice to various microorganisms and to challenge with syngeneic tumor cells was evaluated. the mice received sc injections of 50 microliter corn oil alone or of corn oil containing the equivalent of 0.2, 1, and 4 mg des/kg x d for 14 d. three days later they were challenged with listeria monocytogenes, streptococcus sp. influenza virus, herp ... | 1983 | 6620414 |
| study of acute trichinosis in ghurkas: specificity and sensitivity of enzyme-linked immunosorbent assays for igm and ige antibodies to trichinella larval antigens in diagnosis. | the enzyme-linked immunosorbent assay (elisa) was successfully applied to detect ige antibodies against parasitic antigens by using an additional antibody layer to attain an amplification effect. the sera of 18 gurkha patients with clinical manifestations of acute trichinosis and 35 chinese with other parasitic infections were tested for antibodies to trichinella spiralis by ige-, igm- and igg-elisa, igg-radioimmunoassay (ria) and indirect haemagglutination test (iha). elisas for detection of ig ... | 1983 | 6623599 |
| enteral and parenteral responses in mice after primary infection with trichinella spiralis (nematoda). | enteral and enteral-parenteral infections were produced with t. spiralis in albino, swiss webster, outbred mice. primary enteral infections abbreviated with thiabendazole stimulated inflammatory changes in peyer's patches and the lamina propria of the small intestine of mice. these changes were accompanied by increased iga in the intestinal luminal wash. primary enteral-parenteral infections similarly stimulated the gut, and, in addition, the spleen. splenic stimulation resulted in production of ... | 1983 | 6631634 |
| first report of trichinella spiralis var. pseudospiralis in north america. | | 1983 | 6631644 |
| preliminary observation on the absence of globule leucocytes in mast cell-deficient w/wv anemic mice after trichinella spiralis infection. | | 1983 | 6645137 |
| trichinella spiralis: changes in leucocytes during infection. | rats infected with trichinella spiralis were examined during the course of infection for various changes in the leucocytic population. in each experiment rats were divided into three groups: group a, inoculated with escherichia coli b-5-lipopolysaccharide (lps) administered four days before each experiment; group b, infected with trichinella spiralis; and group c, untreated controls.an extensive leucocytosis was observed in the peripheral blood and peritoneal cavity of infected rats. regardless ... | 1983 | 6655722 |
| stage specific secreted and somatic antigens of trichinella spiralis. | infective larvae, adult males and newborn larvae of trichinella spiralis were cultured with [35s]methionine in vitro. total secreted and total somatic (sodium deoxycholate-soluble) proteins were analyzed by electrophoresis in the presence of sodium dodecyl sulphate (sds-page). secreted proteins were relatively few in number and were different for each stage, whereas somatic proteins gave an unresolved smear in all cases. immune precipitation with serum from infected mice revealed only one major ... | 1983 | 6656844 |
| a comparison of the effects of flubendazole and thiabendazole on the larvae of angiostrongylus cantonensis, trichinella spiralis, diphyllobothrium erinacei and hymenolepis nana in mice. | flubendazole or thiabendazole were administered orally to mice harbouring larvae of angiostrongylus cantonensis, trichinella spiralis, diphyllobothrium erinacei or hymenolepis nana 5-10, 28-33, 21-26 and 1-6 days post-infection respectively. all the mice infected with a. cantonensis were completely cured after treatment with flubendazole at 5 mg/kg/day for 6 days. no noticeable damage was found in the cerebral hemispheres of the mice treated with flubendazole and examined under a dissecting micr ... | 1983 | 6657308 |
| intestinal trichinella spiralis and trichinella pseudospiralis in germfree and conventional mice. | by indirect hemagglutination (iha) test in germfree (gf) and conventional (cv) mice, better antibody response was found in cv mice infected with 200 t. spiralis larvae. in contrast, when 500 larvae were used for infection, the response of gf mice exceeded that of cv controls. humoral responses of gf and cv mice infected with single or mixed t. spiralis or t. pseudospiralis larvae were examined by iha test with the use of both antigens and by determination of g1, a and m immunoglobulins. cellular ... | 1983 | 6657977 |
| rat mononuclear cells and neutrophils are more effective than eosinophils in antibody-mediated stage-specific killing of trichinella spiralis in vitro. | the capacity of non-infected rat total, eosinophil-enriched and eosinophil-depleted fractions of peritoneal exudate and bone marrow cells to adhere to and kill trichinella spiralis newborn larvae with immune rat serum has been studied in vitro. the eosinophil-depleted peritoneal exudate cell fraction contained mainly mononuclear cells, whereas the corresponding bone marrow cell fraction consisted of a considerable number of neutrophils. all cell types either originating from the peritoneal cavit ... | 1983 | 6659655 |
| [some indices of organ reactivity in experimental trichinellosis. i. granulocyte reactions in trichinellosis in rabbits]. | the number of leucocytes, neutrophils and eosinophils was determined during one year observation in 34 rabbits after an infection with trichinella spiralis in the doses of 15,000, 20,000 and 30,000 larvae. granulocyte reactions were already observed on the third day after the infection, they lasted at least for 3 months and depended on the intensity of the infection. | 1983 | 6659739 |
| [some indices of organ reactivity in experimental trichinellosis. ii. lysozyme activity in trichinellosis in rabbits]. | in 34 rabbits infected with trichinella spiralis larvae, lysozyme activity of the serum was determined in the period of one year. we have observed an increased enzyme activity both in acute and chronic period of the disease. rabbits infected with heavy doses of trichinella spiralis larvae showed only a minute increase of serum lysozyme activity. | 1983 | 6659740 |
| differentiation of trichinella spiralis spiralis and trichinella spiralis nativa based on resistance to low temperature refrigeration. | a refrigeration technique to differentiate the subspecies, trichinella spiralis spiralis and t. spiralis nativa is described. trichinella spiralis spiralis trichinae in musculature do not survive 48 hours post-refrigeration at -32 degrees c while t. spiralis nativa will survive 72 hours and longer at the same temperature. | 1983 | 6667435 |
| sialic acid deficiency in lectin-resistant intestinal brush border membranes from rats following the intestinal phase of trichinellosis. | maximal binding (bmax) of the lectin, wheat germ agglutinin, by small intestinal brush border membrane is significantly reduced in rats infected with trichinella spiralis. wheat germ agglutinin specificity is for n-acetylglucosamine and sialic acid. whereas total hexosamine and n-acetylglucosaminidase-labile n-acetylglucosamine were comparable in membranes from uninfected as compared with infected rats, the total sialic acid content and neuraminidase-released sialic acid were significantly highe ... | 1983 | 6669161 |
| a survey of susceptibility to infection with trichinella spiralis of inbred mouse strains sharing common h-2 alleles but different genetic backgrounds. | twenty-eight different inbred strains of mice representing five different h-2 haplotypes were compared for degree of susceptibility to a primary infection with trichinella spiralis. marked differences in susceptibility, measured by the average number of muscle larvae per host, were seen among strains of mice sharing common h-2 alleles. the genes controlling these differences must therefore map at loci outside the major histocompatibility complex. strains of mice sharing the h-2k haplotype were g ... | 1983 | 6674452 |
| comparison of three subspecies of trichinella spiralis by scanning electron microscopy. | the surface morphology of three subspecies of trichinella spiralis was examined by sem in an attempt to find characteristics useful for distinguishing the subspecies. the subspecies studied were t. spiralis spiralis, which had been maintained in swine and laboratory animals for about 50 yr; t. spiralis nativa collected from ursus maritimus at 58 degrees n latitude and 95 degrees w longitude in 1976; and, t. spiralis pseudospiralis, which was derived from the original isolation of this subspecies ... | 1983 | 6674461 |
| expression of expulsion of trichinella spiralis in mice. | | 1983 | 6674465 |
| antigens from trichinella spiralis that induce a protective response in the mouse. | a series of monoclonal antibodies was generated for the purpose of studying antigens of the infective l1 larva of trichinella spiralis. primary immunization of donor balb/c mice was by oral infection with l1 larvae. secondary immunization and screening procedures for hybridoma clones employed a pool of affinity-purified antigens recognized by pooled serum from infected rabbits. three monoclonal antibodies, chosen for their high reactivity in screening assays and their unique specificities, were ... | 1984 | 6690623 |
| trichinella spiralis: rapid, immunologically influenced reduction of intestinal, sodium-coupled sugar transport in the rat. | epithelium of isolated small intestinal segments were studied in ussing-type chambers to detect physiological changes associated with rapid, immune rejection of trichinella spiralis infective larvae. electrophysiological parameters associated with na+-coupled hexose transport were measured. changes in transepithelial electrical potential difference (pd), resistance, and short circuit current (isc) due to the addition of actively absorbed beta-methyl-d-glucoside (bmg) to the mucosal solution were ... | 1984 | 6692878 |
| schistosoma mansoni, s. haematobium, and s. japonicum: identification of genus-, species-, and gender-specific antigenic worm glycoproteins. | immunoreactive glycoproteins of schistosoma mansoni, s. haematobium, and s. japonicum adult worms which are genus-, species-, and gender-specific, or react with sera of patients infected with other parasites, have been identified. schistosomes were metabolically labeled with [35s]methionine and the concanavalin-a binding glycoproteins that were immunoprecipitated with sera of patients infected with one of three species of schistosoma or trichinella spiralis, taenia solium, echinococcus granulosu ... | 1984 | 6692879 |
| scavenger enzymes and resistance to oxygen mediated damage in trichinella spiralis. | in order to understand why different stages of trichinella spiralis vary in their susceptibility to killing by leukocytes, the effects of artificially generated oxidants on different stages of this parasite were compared. more than 90% newborn larvae were killed after incubation in acetaldehyde-xanthine oxidase or glucose-glucose oxidase. on the other hand, fewer than 10% of adult worms or muscle larvae were killed when incubated under identical conditions. thus, only the stages which are resist ... | 1984 | 6694669 |
| [epidemiology of trichinella spiralis infections in the eifel region, 1982]. | in november 1982 146 persons became ill within a few days with trichinosis in bitburg (gfr). prompt epidemiological and immunodiagnostic investigations demonstrated the high likelihood of infection by ingestion of raw sausage produced at a butcher's near bitburg. the diagnosis of trichinosis could be ascertained still in the acute phase of disease by demonstration of the trichinella larvae in peripheral blood. retrospective analysis of earlier disease from the same area showed that the same infe ... | 1984 | 6697913 |
| distribution of trichinella spiralis larvae in selected muscles and organs of experimentally infected swine. | thirty-two hampshire-yorkshire pigs (6 to 8 wk old) were inoculated with the beltsville strain of trichinella spiralis at a level of about 880 larvae/kg of body weight (about 15 kg). at about 100 kg, the pigs were slaughtered and 10-g samples of muscle and other tissues were digested in pepsin-hc1 and examined microscopically for t. spiralis larvae. the mean number of larvae recovered/gram was: tongue, 452; diaphragm, 391; obliquus abdominis internus, 130; serratus ventralis, 116; psoas major, 1 ... | 1984 | 6698907 |