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infectivity and pathogenicity of 14-month-cultured embryonated eggs of toxocara canis in mice.infectivity and pathogenicity to mice of embryonated eggs of toxocara canis, that had been maintained in 2% formalin for 14 months at 4 degrees c, were evaluated by immunological and pathological assessment at 1, 4, 8, 12, 16, 20, 24, 28, 42 and 67 weeks post-infection (wpi). on each date, three infected mice and two age-matched uninfected mice were sacrificed for serum collection and histological processing of the liver, lungs, musculature, and brain. infectivity assessment by enzyme-linked imm ...200312695039
ct and mr imaging findings in cerebral toxocaral disease.we report serial mr findings in a 54-year-old woman with eosinophilic meningoencephalitis due to toxocara canis infection, a parasitic disease contracted through exposure with soil contaminated by the eggs of the roundworm. mr imaging revealed several enhancing subcortical and white matter lesions in both lobes. antihelminthic chemotherapy yielded marked improvement of the neurologic deficits and cerebral lesions. the specific mr findings-low signal intensity on t1-weighted images, high signal i ...200312695211
[kimura's disease on the hard palate in a patient from martinique].we report an observation of kimura's disease (angiolymphoid hyperplasia with eosinophilia) in a black patient from west indies, in a rare localisation on hard palate.200312706782
human toxocariasis and direct contact with dogs.toxocariasis in man is traditionally thought to be contracted through the ingestion of eggs from contaminated soil. the disease may manifest itself in different syndromes such as ocular larval migrans, visceral larval migrans and covert toxocariasis. this paper assesses the evidence for the soil contamination hypothesis and proposes that direct contact with dogs may provide a better explanation of the epidemiology of the disease. hair was collected from 60 dogs from various places in ireland and ...200312708589
cerebellar ataxia due to toxocara infection in mongolian gerbils, meriones unguiculatus.we assessed the usefulness of gerbils as an experimental model for neurologic toxocarosis. mongolian gerbils, meriones unguiculatus, infected with toxocara canis or toxocara cati (1000 eggs/gerbil) showed progressive neurologic disorders from 50 days after infection in t. canis-infected gerbils or from 120 days after infection in t. cati-infected gerbils. the incidence of the onset was 6 of the 13 gerbils (49%) in the t. canis-gerbils and 5 of the 7 gerbils (71%) in the t. cati-gerbils. histopat ...200312719137
an estimation of toxocara canis prevalence in dogs, environmental egg contamination and risk of human infection in the marche region of italy.the human risk of infection with larvae of toxocara canis was estimated in people from the marche region of italy. this region includes both urban and rural areas and its inhabitants frequently keep dogs for company, hunting, as guardians or shepherds. t. canis infection was diagnosed in 33.6% out of 295 dogs examined. nearly half of the dogs (48.4%) living in rural areas were found t. canis positive, compared to about one-quarter of the dogs (26.2%) from urban areas. analysis by provenance and ...200312719139
[prevalence of toxocara spp in public playgrounds in a town of central italy]in this study, the prevalence of toxocara spp. was evaluated in the soil of 22 public playgrounds of ancona (italy). the public playgrounds were selected according to the different areal distribution (centre, low-lying areas centre, hilly areas suburbs) within the urban area of ancona, central italy. in six playgrounds there were signs restricting access for dogs; all of them were frequented by children. parasites were found in the soil samples from 21 out of 22 playgrounds (95.5%). the most pre ...199912736552
[cerebral vasculitis secondary to toxocara canis and fasciola hepatica co-infestation].neurological complications of cosmopolitan parasitosis are rare. we report a case of a cerebral and systemic vasculitis secondary to a visceral larva migrans syndrome associated with a toxocara canis and fasciola hepatica co-infestation.200312773876
human toxocariasis and ascariasis: concomitant parasitism in srinagar, kashmir, india.human toxocariasis caused by toxocara canis is common in both developing and developed countries and leads to visceral larva migrans with high morbidity and mortality. ascariasis caused by ascaris lumbricoides, too has global distribution and in india, high prevalence rate has been reported in kashmir (j & k state). both nematode parasites, toxocara canis and a. lumbricoides require similar biological and environmental conditions for the development of eggs in soil. therefore, the present study ...200212785174
[investigation on zoonoses among veterinary technicians].we conducted a survey on how much veterinary clinics staff, mostly veterinary technicians, know about zoonosis and how extensively they inform pet owners of ways to prevent zoonosis. the number of people who took part in the survey was 1,057. the ratio of people who said their knowledge on zoonosis is "neither sufficient nor insufficient" was 53.8%, which is larger than the ratio of people who replied "sufficient" or "insufficient." the percentage of those who said their way of giving informatio ...200312806928
inorganic pyrophosphatase in the roundworm ascaris and its role in the development and molting process of the larval stage parasites.inorganic pyrophosphatase (ppase) is an important enzyme that catalyzes the hydrolysis of inorganic pyrophosphate (ppi) into ortho-phosphate (pi). we report here the molecular cloning and characterization of a gene encoding the soluble ppase of the roundworm ascaris suum. the predicted a. suum ppase consists of 360 amino acids with a molecular mass of 40.6 kda and a pi of 7.1. amino acid sequence alignment and phylogenetic analysis indicates that the gene encodes a functional family i soluble pp ...200312823552
establishment and migration pattern of toxocara canis larvae in chickens.migrations of toxocara canis larvae were observed in experimentally infected chickens. three groups of three chickens were inoculated orally with t. canis eggs. within each group, individual chickens received either 5,000, 10,000, or 20,000 eggs. a group of infected chickens was then necropsied at either 1, 3 or 6 days post infection (dpi). the entire duodenum, spleen, liver, heart, lungs, right inner pectoral muscle, and brain were subjected to pepsin digestion for larval recovery. larvae were ...200312827506
[chronic urticaria: what problems for the physicians?].chronic urticaria is a common disorder which can be extremely distressing and disabling for the patients. anamnesis and clinical investigations are very helpful for the diagnosis. bacterial, viral or parasitic infections can be associated with chronic urticaria, although the mechanisms which may link infectious diseases and chronic urticaria remain unclear. recently, direct relationship between chronic urticaria and helicobacter pylori, hepatitis c virus and toxocara canis infections have been p ...200312843803
[chronic urticaria and infectious diseases].infectious diseases are often considered as a classic cause of chronic urticaria. nevertheless, laboratory investigations greatly vary from one centre to the other and the link between the infection and skin signs does not rely on hard data. the purpose of this work was a systematic analysis of the published cases of urticaria associated with infection.200312843809
the use of polysiloxane/polyvinyl alcohol beads as solid phase in igg anti-toxocara canis detection using a recombinant antigen.immunodetection of human igg anti-toxocara canis was developed based on elisa and on the use of polysiloxane/polyvinyl alcohol (pos/pva) beads. a recombinant antigen was covalently immobilized, via glutaraldehyde, onto this hybrid inorganic-organic composite, which was prepared by the sol-gel technique. using only 31.2 ng antigen per bead, a peroxidase conjugate dilution of 1:10,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. this procedure is comparable t ...200312886422
[seroepidemiological studies of zoonotic infections in hunters in southeastern austria--prevalences, risk factors, and preventive methods].the aim of this study was to investigate the seroprevalences to zoonotic pathogens in hunters, to propose preventive measures and to obtain more information about the occurrence of zoonotic pathogens in local wild animal populations. from 146 male and 3 female hunters originating from the south-eastern austrian federal states of styria and burgenland blood samples were taken and anamnestic data were obtained using a questionnaire. the serological investigations included the following viral, bact ...200312894685
[helminthes infections in the small intestine of stray dogs in ejungbu city, kyunggi do, korea]one hundred and two stray dogs in ejungbu city of kyunggi-do, korea were examined to reveal out the degree of natural helminthic infection of small intestine. helminthes were collected at autopsy, by scraping the intestinal contents. the collected worms were classified by their morphological characteristics. out of 102 examined, 72 dogs were infected with any helminthes. the common helminthes were dipylidium caninum (47%), ancylostoma caninum (26%), toxascaris leonina (16%) and toxocara canis (1 ...198112902719
[an epidemiological study on zoonoses in korea]helminthic zoonoses are of significant importance to the public health and to the socioeconomic consequences especially in lost-meat, animal products and animal labor. for past several decades in korea, it has been recognized that endoparasitic infections among domestic animals are extremely common and many of the helminth parasites of man have been acquired from, or are shared with domestic animals. however, there was no survey of a nationwide scale on the prevalence of animal helminthiasis; an ...198112902720
[studies on the inducing possibility of human visceral larva migrans associated with eating habit of raw liver of domestic animals]to observe the possibility of human visceral larva migrans due to eating of raw liver of domestic animals, especially of cattle, and also to serve as a good reference for adequate sanitary measures, the investigation survey was carried out from may 1975 to may 1976. from the subjects of a l,048 inhabitants (male 558, female 490) in five localities including two provinces and three different cities, food habit was studied by questionnaire mannual. larvae isolated from liver tissues of cattle, and ...197612913451
[experimental study on the effect of cortisone in mice infected with toxocara canis: histopathological findings of granuloma in the liver]the present study was carried out to examine the effect of "cortisone" on the worm burden migrated in the organs, and granulomatous change in the liver of mice infected with toxocara canis eggs. daily does of 0.5 mg "cortisone" was administrated subcutaneously to toxocara-infected mice with different schedule. the results are summarized as follows: 1. more number of toxocara larvae migrated into the muscle tissue, especially into the carcass through the wall of the large intestine and higher dis ...197412913474
[studies on the intradermal reactions with the fractions of ascaris lumbricoides]introduction : the studies on allergic reaction with the substances of ascaris lumbricoides have long been studied by various worers; conventry(1929), campbell(1936), sakei(1949), miyakawa(1950), ikeda(1952), matsumoto and imawari(1952), morishita and kobayashi(1953, 1954), komiyayama(1954) and yammoto(1956). campbell(1936) and some other workers reported that the polysaccharides from ascaris produced the stronger intrademal reactions than protein fraction, though yamamoto(1954) and others found ...196712913560
[experimental studies on the efficacy of thiabendazole against the migratory stages of ascarids in mouse]introduction: it is known that the larvae of ascarids have migrating phase before they reach the intestine. stewart (1916) reported the pulmonary migration of ascaris larvae in normal host. beaver et al. (1952) demonstrated the ascaris larvae of animal origin from the biopsied human liver, and applied the term "visceral larva migrans" to the migration of larval nematodes in unsuitable hosts. either in normal or abnormal host, the migrated larvae cause inflammatory changes in the tissues and prod ...196712913561
diagnosis of parasitic zoonoses by immunoenzymatic assays--analysis of cross-reactivity among the excretory/secretory antigens of fasciola hepatica, toxocara canis, and ascaris suum.several parasitic infections such fasciolosis, toxocariosis or ascariosis are important zoonoses. during the infection with fasciola hepatica, toxocara canis and ascaris suum, an important intraorganic phase in their hosts takes place, releasing antigens responsible for a humoral immune response, which enables the diagnosis of that parasitosis. a study to identify the existence of cross-reactivity among the excretory/ secretory antigens of f. hepatica, t. canis and a. suum was developed. one gro ...200312916704
endoparasites in dogs and cats in germany 1999-2002.infections with endoparasites in dogs and cats have been determined by analysing the results of faecal examinations (flotation, mifc, sedimentation, baermann, smear, prospect giardia microplate assay). samples of 8438 dogs and 3167 cats from the years 1999 until 2002 have been included in the investigation. 2717 dogs (32.2%) and 771 cats (24.3%) have been infected with endoparasites. in the infected dogs the following parasites have been identified: class nematodea: toxocara canis: 22.4%, toxasc ...200312928886
synergistic effects of pyrantel and the febantel metabolite fenbendazole on adult toxocara canis.pyrantel embonate and febantel are both constituents of drontal plus and drontal puppy broad spectrum anthelmintics for dogs. the effects of pyrantel and the febantel metabolite fenbendazole were investigated against toxocara canis in-vitro by studying changes in worm motility and tissue damage. pyrantel and fenbendazole were added to worms incubated in media for 8 h at the following concentrations: pyrantel: 12.2 microg, 25 microg, or 50 microg; fenbendazole: 50 microg, 100 microg or 200 microg ...200312928887
epidemiology of toxocara canis in the dog population from two areas of different socioeconomic status, greater buenos aires, argentina.toxocara canis infection in dogs is a public health problem in most countries, although it has been poorly documented in many of them. the main objective of the present work was to investigate the epidemiology of infection in the canine populations from two areas of buenos aires of different socioeconomic status and urban conditions: a middle-income neighbourhood (min) and a low-income neighbourhood (lin). this study evaluated the prevalence of infection in dogs by parasitological and serologica ...200312935743
[frequency of seropositivity to toxocara canis in children of different socioeconomic strata].frequency of seropositivity for toxocara in children from different socioeconomic strata in the city of brasilia (brazil) was measured. six hundred and two children of both sexes, aged one to 12 years were distributed in two socioeconomically distinct groups. the samples of sera of the first group were obtained from blood drawn for routine tests in the laboratory of a public hospital attending children from low-income families. samples from the second group were obtained from private laboratorie ...200312937730
disodium cromoglycate prevents ileum hyperreactivity to histamine in toxocara canis-infected guinea pigs.the aim of this study was to investigate whether toxocara canis infection in guinea pigs provokes changes in ileum responsiveness to histamine. ileum segments from control and t. canis-infected groups were placed at isometric conditions and submitted to various doses of histamine. no changes were observed between controls and t. canis-infected groups at days 3, 6 and 12 after infection. however, at days 18 and 24 after infection, there was a significant increase in ileum responsiveness to histam ...200312967589
dog roundworm infestations in children. 195513262264
[pathological changes of the intestinal wall adjacent to conglomeration of toxocara canis]. 195513296680
amebic invasion of the liver of guinea pigs infected with the larvae of a nematode, toxocara canis. 195613365620
the detection of antibodies to infections with the nematode, toxocara canis, a causative agent of visceral larva migrans. 195713435432
a contribution to the life history and larval morphology of toxocara canis. 195713492113
intraocular nematodiasis; with special reference to toxocara canis, the dog ascarid, as a causative agent in nematode endophthalmitis. 195813533497
observations on the development of toxocara canis (werner, 1782) in the dog. 195813566864
a survey of toxocara canis and toxocara cati prevalence in the new york city area. 195813578117
nematode endophthalmitis: toxocara canis: report of one case. 195913649858
an evaluation of piperazine citrate for preventing prenatal infections with the common dog ascarid (toxocara canis). 195913664613
influence of pregnancy and lactation on migration of the larvae of toxocara canis in mice. 196113731422
standardization of techniques for infecting mice with toxocara canis and observations on the normal migration routes of the larvae. 196113731423
effects of superinfection on the behavior of toxocara canis larvae in mice. 196013760231
[eosinophilia during infection with ascaris lumbricoides and toxocara canis (larva migrans)]. 196013802107
tropical eosinophilia. experiments with toxocara canis. 195913809479
organ disturbution of toxocara canis larvae in normal mice and in mice previously infected with toxocara, ascaris or trichinella. 196213940084
[determination of the diagnostic value of a test in toxocara canis infestation]. 196213997374
nematode induced hypersenistivity reactions in guinea pigs: onset of eosinophilia and positive schultz-dale reactions following graded infections with toxocara canis. 196314088709
experimental toxocara canis infections in chickens and pigeons. 196414125154
encephalitis due to infection with toxocara canis. report of a suspected case. 196414184146
visceral larva migrans. toxocara canis infection. 196414184147
[contributions to the problem of the migration route of ascarid larvae (ascaris lumbricoides linn'e 1758 and toxocara canis werner 1782) in the experimental and natural host]. 196414208916
toxocara canis infestation. 196414215239
infection by the dog roundworm toxocara canis and fatal myocarditis. 196414234289
toxocara canis infestation: clinical symptoms and signs with report of a case. 196314237252
prenatal toxocara canis infection in pups. 196514256949
antibody to toxocara canis in humans. 196514270133
the resistance of tortoises, testudo graeca l., against toxocara canis infection. 196514272967
effect of diethylcarbamazine, oxophenarsine hydrochloride and piperazine citrate on toxocara canis larvae in mice. 196014432951
human toxocara canis encephalitis with lead encephalopathy. 196214475700
[importance of pars plana vitrectomy in the diagnosis of ocular toxocariasis].a retrospective study evaluates the importance of pars plana vitrectomy (ppv) in four patients with ocular form of toxocarosis. the results of laboratory determination of the titres of antibodies against toxocara canis (the elisa method) in samples of vitreous body were compared with those in the serum of the patient. the individual cases exemplify the problems in interpretation of laboratory findings and document the authors' experience in considering the diagnosis of ocular toxocarosis in indi ...200314518357
[diagnosis of toxoplasmosis and toxocariasis using fluid from the anterior ocular chamber].the authors discuss methods of improvement of the etiologic diagnosis of the toxoplasma and toxocara associated posterior uveititis, by examining specific antibodies in the aqueous humor. paired samples of aqueous humor and serum were analyzed in 23 patients. chorioretinitis of infectious etiology was suspected in 15 patients, 3 patients were treated for panuveitis, 4 patients for intermediate uveitis, and in 1 patient the sample was obtained to rule out malignant melanoma. the local ocular anti ...200314518358
influence of maternal infection on offspring immune response in murine larval toxocariasis.the impact of toxocara canis infection on the proportion of cd4+ and cd8+ t splenocytes, the serum concentrations of cytokine ifn-gamma and il-5, and the production of toxocara-specific antibodies were studied in two c57bl6/j mouse groups and their offspring. the mice were infected with 1000 t. canis eggs on the day of mating (early infection) and on day 14 of pregnancy (late infection). early infection resulted in a significant increase of cd4+ t-cell subtype, however, a decline in cd8+ in comp ...200314521578
lafoensia pacari extract inhibits il-5 production in toxocariasis.toxocariasis is an infection induced by toxocara canis, an intestinal parasite of dogs. in this study, an experimental murine model of toxocariasis was used to evaluate the anti-inflammatory activity of an ethanolic extract of lafoensia pacari stem bark. mice infected with t. canis were treated with l. pacari extract (200 mg/kg, p.o.). subsequently, we observed a reduction in the number of eosinophils in the peritoneal cavity, bronchoalveolar fluid, blood and bone marrow. production of interleuk ...200314521582
mucin-type o-glycosylation in helminth parasites from major taxonomic groups: evidence for widespread distribution of the tn antigen (galnac-ser/thr) and identification of udp-galnac:polypeptide n-acetylgalactosaminyltransferase activity.this article focuses on the initiation pathway of mucin-type o-glycosylation in helminth parasites. the presence of the galnac-o-ser/thr structure, also known as tn antigen, a truncated determinant related to aberrant glycosylation in mammal cells, and the activity of the udp-galnac:polypeptide n-acetyl-galactosaminyltransferase (ppgantase), the enzyme responsible for its synthesis, were studied in species from major taxonomic groups. tn reactivity was determined in extracts from taenia hydatige ...200314533679
ecological analyses of the intestinal helminth communities of the wolf, canis lupus, in spain.this work describes the ecological characteristics of the intestinal helminth communities of 50 wolves (canis lupus l.) from spain. the species found were classified into three groups according to prevalence, intensity and intestinal distribution. taenia hydatigena pallas, 1766 and uncinaria stenocephala (railliet, 1884) are the core species of the community. taenia multiceps (leske, 1780) is a secondary species. the rest of the species, alaria alata (goeze, 1782), taenia serialis (gervais, 1847 ...200314535350
haemonchus contortus: molecular characterisation of a small heat shock protein.a cdna encoding a predicted small heat shock protein, hsp20, was isolated from the parasitic nematode haemonchus contortus. this cdna encoded a predicted protein of 156 amino acids, which had high sequence identity with other nematode small heat shock proteins. southern blot analysis of genomic dna suggested that in h. contortus hsp20 is encoded by a single copy gene. the hsp20 transcript and protein were expressed in the infective larvae (l3), early l4 and adult stages, but expression was not i ...200314552856
identification of tissue-embedded ascarid larvae by ribosomal dna sequencing.polymerase chain reaction (pcr) was applied to identify tissue-embedded ascarid nematode larvae. two sequences of the internal transcribed spacer (its) regions of ribosomal dna (rdna), its1 and its2, of the ascarid parasites were amplified and compared with those of ascarid-nematodes registered in a dna database (genbank). the its sequences of the pcr products obtained from the ascarid parasite specimen in our laboratory were compatible with those of registered adult ascaris and toxocara parasit ...200414598166
is anisakis simplex responsible for chronic urticaria?the association of chronic urticaria (cu) to parasitic infestations has been poorly studied. recently, sensitization to the parasite larva anisakis simplex has been described as the cause of acute urticaria and anaphylaxis. the aim of this work was to study the relationship between sensitization to a. simplex and cu. one hundred one patients with cu were studied. data of possible contacts with a. simplex were collected and the usual cu study was performed. furthermore, total and specific immunog ...200314619334
evidence for the involvement of the optic nerve as a migration route for larvae in ocular toxocariasis of mongolian gerbils.although toxocara canis, an important pathogen of ocular disease, tends to migrate to the eye, the precise migratory route has yet to be determined experimentally. mongolian gerbils, meriones unguiculatus, known as a useful animal model for human toxocariasis, were used to investigate the migration route toward the eyes. infective larvae of t. canis were directly inoculated into the intracranial region. haemorrhagic lesions or larvae were observed in 56.3% of cases. histopathologically, a larva ...200314627447
cross-reactivity induced by anisakis simplex and toxocara canis in mice.the aim of this study was to verify whether cross-reactivity appeared between toxocara canis and anisakis simplex in an experimental rodent model. no cross-reactions were detected using sera from mice infected with t. canis eggs. when responses obtained against t. canis es antigen using sera from balb/c and c57bl/10 mice infected with t. canis eggs were compared with those obtained by testing sera from mice infected with one a. simplex l3, an increase in cross-reactions was observed using the c5 ...200314627450
eosinophilic meningoencephalitis due to toxocara canis: case report and review of the literature.toxocariasis is usually manifested as visceral larva migrans. nervous system involvement is a rare complication. in this report, we describe one case of meningoencephalitis due to toxocara canis and review the literature. we report a previously healthy two-year-old boy who was admitted after 24 hours of severe neurologic symptoms with marked eosinophilic pleocytosis in the cerebrospinal fluid and a single subcortical lesion detected by cerebral magnetic resonance imaging. both serum and spinal f ...200314628955
helminth antigens (taenia solium, taenia crassiceps, toxocara canis, schistosoma mansoni and echinococcus granulosus) and cross-reactivities in human infections and immunized animals.helminth antigens were investigated in the search for accessible heterologous antigens capable to discriminate different helminthiases, by the enzyme linked immunosorbent assay (elisa) and the immunoblot assay (ib). antigens used were: taenia solium cysticercus total saline (tso); taenia crassiceps cysticercus vesicular fluid (tcra-vf); t. crassiceps cysticercus glycoproteins (tcra-gp and tcra-(18-14)-gp); toxocara canis larva excretory-secretory (tes); schistosoma mansoni adult total saline (sm ...200314636985
cross-reactivity between anisakis simplex sensitization and visceral larva migrans by toxocara canis.the aim of this work was to study cross-reactivity in the diagnosis of two related ascaridosis. nineteen patients diagnosed with recidivous acute urticaria (rau) caused by anisakis simplex and 26 patients diagnosed with visceral larva migrans (vlm) caused by toxocara canis were studied employing commercial diagnostic kits and "in house" assay kits. cross-reactivity observed was greater when using "in house" assay kits, suggesting that t. canis excretory-secretory antigens were not only recognize ...200314636986
orbital cellulitis associated with toxocara canis in a dog.reports of toxocara canis ocular larva migrans are uncommon in animals, with only a few cases reported. most reports involve larval migration into the retina and choroid, with parasitic invasion of the orbit reported only in experimental studies. this is the first clinical case of toxocara canis infection in the retrobulbar region of a 10-year-old, cross-bred male dog presenting with unilateral orbital cellulitis. ophthalmic signs included protrusion of the nictitating membrane, chemosis, exopht ...200314641832
prevalence of zoonotic important parasites in the red fox (vulpes vulpes) in great britain.a national necropsy survey of red foxes was carried out across great britain to record echinococcus, trichinella and toxoplasma. the survey did not record directly, or indirectly using coproantigen/pcr tests, evidence for the presence of echinococcus multilocularis in 588 animals, although e. granulosus was suspected in six animals. parasitological evidence for trichinella spp. could not be found in 587 fox muscle digests, and a specific pcr test also failed to detect toxoplasma in a sub-set of ...200314651882
recombinant expression of the larval excretory-secretory antigen tes-120 of toxocara canis in the methylotrophic yeast pichia pastoris.a gene encoding the larval excretory-secretory antigen tes-120 of the dog ascarid worm toxocara canis was cloned into the methylotrophic yeast pichia pastoris. specificity of the recombinant tes-120 antigen produced by the yeast was investigated. forty-five human serum samples from patients infected with different()parasitic organisms, including 8 cases of toxocariasis, were tested against the recombinant antigen in immunoblot assays. results from the assays showed that the recombinant tes-120 a ...200414655048
tissue parasites in patients with chronic urticaria.chronic urticaria is an important diagnostic and therapeutic problem. we aimed to investigate the sero-prevalence of tissue parasites causing toxocariasis and fasciolosis in patients with chronic urticaria. all cases were analyzed for antibodies against toxocara canis and fasciola hepatica by modified (homemade) elisa. the excretory/secretory products of toxocara and fasciola were used as antigens (es-elisa) in the test. in this study, the highest toxocariasis seropositivity (29.0%) rate and the ...200314684933
population dynamics of toxocara canis in pigs receiving a single or multiple infection.the population dynamics of toxocara canis in pigs, and their immune response to a primary and a challenge infection, were studied by parasitological, haematological and serological parameters. seventy pigs were divided into 4 groups; 35 pigs received a primary infection (group a), 15 pigs received both a primary and a challenge infection (group b), 15 pigs received the challenge infection only (group c), and 5 pigs served as helminth-free controls (group nc). a dose of 50,000 eggs was administer ...200314700196
clinical features of ocular toxocariasis in japan.to characterize the clinical features of ocular toxocariasis and to describe the unique aspects of the disease in japan.200314704898
helminth parasites of cats from the vientiane province, laos, as indicators of the occurrence of causative agents of human parasitoses.a total of 55 domestic cats (felis catus f. domestica) and one wild (bengal) cat (prionaluirus bengalensis) from the vientiane province, central laos, were examined for helminth parasites with emphasis given to potential human parasites. the following species were found (parasites infective to man marked with an asterisk): opisthorchis viverrini, haplorchis pumilio, h. taichui, h. yokogawai, stellantchasmus falcatus (digenea); spirometra sp., dipylidium caninum, taenia taeniaeformis (cestoda); c ...200314710631
human toxocariasis: incidence among residents in the outskirts of campinas, state of são paulo, brazil.with the aim of estimating the incidence of infection by toxocara among residents in the outskirts of campinas (state of são paulo, brazil) two serological surveys, using elisa anti-toxocara tests, were performed in january 1999 and january 2000, involving, respectively, 138 and 115 individuals, 75 of which examined in both occasions. among this group 67 individuals did not show the presence of anti-toxocara antibodies in 1999, and 12 presented seroconversion in the second survey, revealing an a ...200314743671
nematodes and water pollution in eastern part of nile delta.examination of some represented water sites revealed presence larvae and eggs of the nematodes infecting man and animals. eggs in a descending order of abundance were trichostrongylus, toxocara canis, t. vitolorum, and ascaris sp., then trichocephalus and ancylostoma sp. larvae in a descending order were strongyloides, trichostrongylus and ancylostoma sp. no doubt contaminated or polluted water plays an important role as nematode-borne source.200314964672
[infections with endoparasites in dogs in dutch animal shelters].faecal samples from 224 dogs from 23 animal shelters in the netherlands were examined for endoparasites. in total 20.5% of the faecal sample were positive for helminth and/or protozoa infections. eggs of toxocara canis were found in 8.5% of the faecal samples. other endoparasites found were toxascaris leonina (0.5%), trichuris vulpis (4.9%), uncinaria stenocephala (2.2%), dipylidium caninum (1.3%), taenia spp. (0.5%), cystoïsospora canis (1.3%), and c. ohioensis (1.3%). dogs younger than 1 year ...200414976680
[effects of cyclosporin a or betamethasone on experimental murine toxocariasis].the effects of administration of either cyclosporin a or betamethasone 15 days before or 45 days after experimental infection with toxocara canis on mice had been studied. the dynamics of igg antibody production, employing an enzyme-linked immunosorbent assay, was studied 60 and 90 days after mice infection by toxocara canis. in the 90th day after infection all surviving mice were sacrificed and the tissue trapped larvae recovered by acid digestion in the muscles, lungs, liver and brain. a signi ...200415042177
prevalence of toxocara canis infection in household dogs.the prevalence of toxocara canis infection in household dogs was estimated by examining feces from 1,743 dogs aged between 1 month and 15 years old. fecal samples from 75 (4.3%) of the 1,743 dogs were positive for the eggs of t. canis. the dogs with positive fecal samples ranged from 1 month to 5 years old in age. the infection rate in dogs aged 1 to 6 months old was significantly (p < 0.01) higher than that in dogs aged 7 months to 2 years old or over 3 years old. indoor dogs aged 1 to 6 months ...200415103902
prevalence of intestinal parasites in dogs under veterinary care in maracaibo, venezuela.the prevalence of intestinal parasites in dogs presented to the veterinary policlinic of the university of zulia (pvu) was measured between january and december 2001. a total of 614 fecal samples were evaluated by the fecal flotation method. one or more species of parasites was identified in 218 (35.5%) dogs. the parasites most frequently detected were: ancylostoma spp. (24.5%), toxocara canis (11.4%) and isospora spp. (8.1%). single parasitic infections were present in 149 (24.3%) dogs. the age ...200415110399
zoonotic risk of toxocara canis infection through consumption of pig or poultry viscera.the potential zoonotic risk of toxocara canis infections from consumption of swine or poultry viscera containing larvae was assessed using a pig model. two groups of six pigs were fed either fresh swine viscera (group fs) or poultry viscera (fp) containing around 3500 toxocara larvae. another two groups of six pigs were fed swine viscera (ps) or poultry viscera (pp) preserved at 4 degrees c for 1 week. all pigs were necropsied 14 days after the exposure. liver white spots were counted and t. can ...200415110409
recombinant expression of toxocara canis excretory-secretory antigen tes-120 in escherichia coli.the gene encoding the excretory-secretory antigen tes-120 of dog ascarid worm toxocara canis was cloned into the bacterium escherichia coli. the specificity of the recombinant tes-120 antigen produced by the bacterium was investigated. a total of 45 human serum samples from patients infected with differenthelminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. the results from the assays revealed that the recombinant tes-120 ...200315115078
the infectivity and antigenicity of toxocara canis eggs can be retained after long-term preservation.suspensions of fertilized eggs of toxocara canis were mixed with 2% neutral formalin and preserved at 4 degrees c. when, after storage for 0, 12, 18, 21 and 24 months, samples of the eggs were incubated at 30 degrees c for 12 days, 96.8%, 92.6%, 74.1%, 51.0% and 19.3% of the eggs in the samples were found to embryonate. the embryonated eggs produced from the fertilized eggs preserved (in 2% neutral formalin at 4 degrees c) for 0, 12, 18 and 21 months were then tested for their infectivity to bal ...200415119970
cerebral toxocariasis: a possible cause of epileptic seizure in children.toxocariasis is a worldwide human helminthiasis, which is mostly asymptomatic and caused by toxocara canis, a roundworm in dogs. these can cause visceral larva migrans syndrome in humans who ingest contaminated soil. cns manifestation with a focal mass lesion is very rare, seizures often being the first symptom.200415138789
['petting dogs can cause blindness': a critical analysis]. 200415185619
cross-reactivity between antigens of anisakis simplex s.l. and other ascarid nematodes.a study of the cross-reactivity among somatic and excretory-secretory antigens of the third stage larvae of anisakis simplex s.l. and somatic antigens of other ascarid nematodes (ascaris lumbricoides, a. suum, toxocara canis, anisakis physeteris, hysterothylacium aduncum and h. fabri) was carried out by immunoblotting. it was revealed a high degree of cross-reactivity among ascarids in the 30 and > 212 kda range by using sera against somatic and excretory-secretory antigens of a. simplex s.l. it ...200415224584
the frequency of toxocara infection in mental retarded children.human toxocariasis is commonly seen in places where stray and toxocara canis-infected dog population is high. there is a strong correlation between frequency of toxocara infection, life style, and infection risk. institutionalization of mental retarded patients increases to risk of toxocariasis. in this study, we aimed at investigating the frequency of toxocara infection among children with mental retardation not requiring institutionalization. the study included 96 cases, who had educatable men ...200415250462
[ocular parasitosis--particular aspects].the goal of this study cases are presented in this study. the first case was emphasized in a patient with an epibulbar tumor and the second case was diagnosed with toxocara canis. in both cases the ophthalmoscopic images and the clinical evolution were unusual on the other hand etiological diagnosis in both cases was difficult. in the first case, the surgical procedure was effective, but in the second case the specific antiparasitic treatment could not stop the evolution of corioretinal's lesion ...200415279423
characterization of biological activities of feline eosinophil granule proteins.to characterize eosinophil granule-derived proteins in cats.200415281655
[liver resection for a rare parasitic infection--visceral larva migrans syndrome].the authors describe a case of an 38-year-old woman suffering from a parasitical infection which is rare in hungary. it was diagnosed in connection with a surgical liver segment resection. visceral larval migrans is an infection caused by migration of the roundworm toxocara larvae to organs and tissues. the authors describe the pathophysiology of the disease, clinical symptoms, diagnostic and therapeutic possibilities.200415285152
[results of parasitological examinations of faecal samples from horses, ruminants, pigs, dogs, cats, hedgehogs and rabbits between 1998 and 2002].the results of coproscopical examinations in horses, ruminants, pigs, dogs, cats, hedgehogs and rabbits between 1998 and 2002 are presented. in 4399 samples from horses 37.4% stages of strongylids, 1.4% anoplocephalids, 1.3% strongyloides westeri, 0.9% parascaris equorum, 0.04% oxyuris equi, 0.04% eimeria sp. and 0.04% fasciola hepatica were found. in 998 samples of cattle 22.1% stages of strongylids, 11.2% of eimeria spp., 3.5% of cryptosporidium, 2.9% of moniezia spp., 1.3% of trichuris spp., ...200415287577
seroepidemiology of toxocara canis infection among mountain aboriginal adults in taiwan.seroepidemiology of toxocara canis infection among adults of one ethnic han and five aboriginal populations residing in mountainous areas of taiwan was conducted by detecting serum igg (>/=1:64) using a t. canis larval excretory-secretory antigen-based enzyme-linked immunosorbent assay. a short questionnaire interview was conducted to obtain data concerning their age, sex, occupation, consumption of raw pig liver, and possession of dogs. the overall seroprevalence (46.0%, 247 of 537) in the five ...200415306714
[diagnostics of larval toxocariasis].the paper, conceived as a retrospective study, resumes the results of our own investigations of the presence of serum igg antibodies against toxocara canis (as a crossover also against t. cati) in the sera of patients suspected of presenting larval toxocariasis in the region of eastern bohemia of the czech republic in the years 1997-2002. our aim was to determine the immunity rate of igg, its relationship to age and sex, further the whole range of diagnoses, the frequency of demands for such an ...200415328575
[negligible risk of visceral or ocular larva migrans from petting a dog].in a recent publication it was suggested that human toxocarosis, including ocular larva migrans, may result from petting dogs infected with toxocara canis, the dog roundworm. recalculating the eggs per gram in the dog's coat revealed that in 7 of the 15 positive dogs only one toxocara egg per dog was found. only 4% of all eggs were embryonated and hence infectious. it is, however, very plausible that this is contamination originating from the environment. the authors found also many other parasi ...200415382563
clinical quiz. toxocara canis infection with hepatic and lung involvement. 200415448438
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