| late-phase bronchial vascular responses in allergic sheep. | sheep were classified on the basis of their airway response to ascaris suum antigen aerosols as allergic or nonsensitive. allergic sheep were classed as acute or dual responders. acute responders had only an immediate increase in mean airflow resistance after antigen, whereas dual responders had an immediate and late-phase (6-8 h after antigen challenge) increase in mean airflow resistance; nonsensitive sheep had minimal airway responses to antigen (less than 30% increase from base line). the sh ... | 1990 | 2228869 |
| the relationship between the frequency distribution of ascaris lumbricoides and the prevalence and intensity of infection in human communities. | observed field data from a range of geographically distinct human communities suggest a consistent non-linear relationship between prevalence and mean intensity of ascaris lumbricoides infection. utilizing the negative binomial distribution as a description of observed aggregation, maximum-likelihood analysis reveals that the degree of aggregation is a negative linear function of mean worm burden. the factors responsible for this relationship in human populations require further study but may in ... | 1990 | 2235069 |
| evidence for the frequent use of ttg as the translation initiation codon of mitochondrial protein genes in the nematodes, ascaris suum and caenorhabditis elegans. | data obtained from alignments of nucleotide sequences of mitochondrial (mt) dna molecules of the nematode worms ascaris suum and caenorhabditis elegans indicate that in six of the mt-protein genes of a. suum and three of the mt-protein genes of c. elegans ttg is used as the translation initiation codon. also, gtt seems to be the translation initiation codon of the a. suum coiii gene. all of the five remaining a. suum mt-protein genes appear to begin with att and the remaining nine c. elegans mt- ... | 1990 | 2235493 |
| health status of children living in a squatter area of manila, philippines, with particular emphasis on intestinal parasitoses. | in 1988 a study was performed in a squatter area of manila, philippines. three substudies were carried out: (1) an analysis of the reported health situation and the available health services for the population of smokey mountain, (2) a vaccination coverage survey and (3) a cross-sectional stool sample survey. respiratory infections, diarrhea, measles and malnutrition were the most frequently reported causes of ill-health and death. not only governmental but also various non-governmental health s ... | 1990 | 2237597 |
| effects of multiple dose infections with ascaris suum on blood gastrointestinal hormone levels in pigs. | ten consecutive daily doses of infective ascaris suum eggs were administered to pigs in two experiments and the levels of gastrointestinal hormones in their blood were measured. the piglets in each experiment were divided into low-dose (ldi) and high-dose (hdi) infections and control groups. infected pigs had lower feed consumption, lower weight gains, and lower feed efficiency than control pigs. serum gastrin levels in infected pigs were significantly lower than the controls from days 7 to 17 p ... | 1990 | 2238428 |
| the monitoring of foodhandlers in papua new guinea. | in early 1987 guidelines were re-enforced for pre-employment medicals and 6-monthly health checks on foodhandlers in the ok tedi mining project in papua new guinea. the health monitoring program was stepped up as a result of two typhoid cases imported from the highlands and catering subcontractors failing to comply with the necessary pre-placement medicals for foodhandlers. highlanders made up 28% (49/174) of the ok tedi catering department's workforce in 1987. the initial screen of 155 foodhand ... | 1990 | 2238835 |
| a coprological survey of parasites of wild mantled howling monkeys, alouatta palliata palliata. | fecal samples from 155 mantled howling monkeys (alouatta palliata palliata) examined at centro ecologico la pacifica, guanacaste province, costa rica, revealed 75 (48%) had parasitic infections. a sampling of nine howling monkeys from santa rosa national park. costa rica indicated only one infected animal (11%). only three of 19 (16%) spider monkeys (ateles geoffroyi) also from santa rosa were infected. controrchis biliophilus, trypanoxyuris minutus, unidentified strongylid eggs and isospora sp. ... | 1990 | 2250334 |
| [trypsin and alpha-amylase activities in the pancreas of guinea pigs. ii. effect of per os and intra-intestinal infection with invasive ova of ascaris suum]. | the guinea pigs were infected orally or intraintestinally (after laparotomy) with 5000 invasive eggs of ascaris suum. on the 7th day after infection the invasion was controlled by lung's weight, degree of congestion, and number of larvae in the lungs. the activity of amylase was determined according to fennel method, trypsin to anson method. the activities of both enzymes in pancreas of orally infected animals (after laparotomy or not) were lower, and relative weight of lungs of these animals wa ... | 1990 | 2256335 |
| [trypsin and alpha-amylase activities in the pancreas of guinea pigs. iii. effect of homogenates of invasive ova of ascaris suum and organic acids c2-c6 on the activity of enzymes in pancreatic extracts]. | the studies were carried out on pancreas extracts from guinea pigs free of parasite invasion. the activity of trypsin was determined according to the method of anson, and amylase of fennel method. the measurements of activity were done at the presence of homogenized invasive eggs of ascaris suum, and the mixture of volatile organic acids c2-c6 (at concentrations of 30.3 and 0.3 mm/l). in samples with homogenized invasive eggs of a. suum the amylase activity remained unchanged while the activity ... | 1990 | 2256339 |
| intestinal ascariasis in the differential diagnosis of peptic ulcer disease. | 865 patients who had barium meal examination for clinical suspected peptic ulcer disease were studied. out of these, 175 patients (20.2%) had no radiological evidence of peptic ulcer disease but were found to be infested with ascaris worms and their symptoms abated with the use of anthelminthics. none of the 93 patients who had haematemesis and gastric outlet obstruction had intestinal ascariasis. it is therefore suggested that in patients with symptoms of peptic ulcer disease, provided there ar ... | 1990 | 2260195 |
| [helminth infections in wild boars in enclosures in southern germany: species spectrum and infection frequency]. | to determine the helminth fauna of wild boars, stomachs and intestines of 124, lungs of 62, and livers of 39 animals from 5 enclosures in southern germany were examined. the evaluation of the species composition revealed at least 13 species: metastrongylus apri, m. salmi, m. pudendotectus, physocephalus sexalatus, hyostrongylus rubidus, globocephalus longemucronatus, ascaris suum, trichuris suis, oesophagostomum dentatum, o. quadrispinulatum, fasciola hepatica, c. tenuicollis and capillaria. the ... | 1990 | 2264058 |
| prevalence of intestinal parasitoses in relationship to diarrhoea among children in ilorin. | studies on the role of intestinal parasites in diarrhoeal disease in children were carried out among 907 children of one month to 120 months (10 years) of age both at out patients and inpatients in unilorin teaching hospital. the most common intestinal parasites found were ascaris lumbricoides (40.9%), trichuris trichiura (27%), hookworm (10.2%), strongyloides stercoralis (9.7%), e. histolytica (6.3%), trichomona vaginalis (2.8%). taenia saginata (1.7%) and 1.1% of paragonimus westermani, among ... | 1990 | 2268573 |
| molecular cloning and expression of an immunodominant 53-kda excretory-secretory antigen from trichinella spiralis muscle larvae. | a trichinella spiralis cdna expression library was constructed in lambda gt11 from muscle larvae mrna and immunologically screened to identify genes encoding previously described immunodiagnostic excretory-secretory (es) antigens. screening the library with t. spiralis infection serum from swine or rabbit antiserum to t. spiralis es antigen identified one clone, designated tsa-12, that contains a cdna transcript 539 bp in length and codes for an apparent 123-kda beta-galactosidase fusion protein ... | 1990 | 2270099 |
| [intestinal parasite infections among inhabitants in two islands of tongyeong-gun, kyeongsangnam-do]. | this study was performed to evaluate the status of intestinal parasitic infections among the inhabitants in two islands (chu-do and doomi-do) of tongyeong-gun, kyeongsangnam-do (province), from august to september, 1989. a total of 189 stool specimens was collected from the inhabitants of 3 villages and examined by kato's cellophane thick smear and formalin-ether sedimentation techniques. stoll's dilution egg counting technique was done for the quantitative examination of helminth eggs. the over ... | 1990 | 2271502 |
| status of intestinal parasite infections in inhabitants of palmar, guayas province, ecuador. | status of intestinal protozoan and helminthic infections was surveyed in mestizo population living in rural parish of palmar and its nearby recintos(villages). three of the surveyed villages were at the pacific coast and 5 villages were in inner pasture land, located about 100 km west of guayaquil, the second largest city in ecuador. one stool sample was examined by one cellophane thick smear for helminth ova and one direct smear stained with lugol's iodine solution for protozoan cysts. of 325 p ... | 1990 | 2271503 |
| [parasite prevalence in schools with different socioeconomic status and evaluation of methods for diagnosing intestinal parasitic diseases]. | in this study; the parasite prevalence of schools with different socio-economical status and three methods for diagnosing intestinal parasitosis were evaluated. children from two primary schools and one junior high school with different socio-economical status participated in the study. the three method used for diagnosing parasitosis in this study were direct wet mounts, concentrated saline flotation technique, and zinc sulfate centrifugal flotation method. parasitosis was found in 69 fecal sam ... | 1990 | 2287296 |
| [digestive parasitic diseases in young children in a tropical outpatient setting]. | parasites were looked for in stools of infants aged 6 months to 3 years living in a village in the south of togo. these children were enrolled in a nutritional surveillance program. 42.5% of children harboured at least one parasite and 12.1% had more than one parasite: the most common parasites found included giardia (21%), ancylostoma (13%), and ascaris (12.5%). infection with a single parasite and infection with several parasites were observed from the age of nine months and 18 months, respect ... | 1990 | 2291596 |
| ascaris lumbricoides: detecting its metabolites in the urine of infected people using gas-liquid chromatography. | substances dissolved in the urine of people infected with ascaris lumbricoides were extracted and detected by gas-liquid chromatography. the ratios of the areas of the peaks produced by two substances extracted from urine to the area of the peak of solvent were found to be significantly correlated with the worm burden. a chemical analysis of the predominant substance by infrared spectrophotometry and proton magnetic resonance spectrometry indicated that it was 2-methyl-butyramide. the chemical p ... | 1990 | 2295325 |
| effect of nonadrenergic noncholinergic inhibitory nerve stimulation on the allergic reaction in cat airways. | to examine the effect of nonadrenergic noncholinergic (nanc) inhibitory nerve stimulation on the antigen inhalation with allergic animals, changes in pulmonary resistance (rl) and arterial plasma histamine concentration ([h]) caused by inhalation of ascaris suum antigen were studied in five control (group a) and five nerve-stimulated (group b) cats, which were anesthetized and mechanically ventilated. all animals were actively sensitized with ascaris antigen before the experiment. after choliner ... | 1990 | 2297183 |
| transcription and cap trimethylation of a nematode spliced leader rna in a cell-free system. | maturation of a fraction of mrnas in nematodes involves the acquisition of a common 5' terminal spliced leader sequence derived from a nonpolyadenylylated spliced leader rna by trans splicing. we have developed a cell-free system prepared from ascaris lumbricoides embryos that accurately and efficiently synthesized the spliced leader rna of a. lumbricoides. transcription of the spliced leader rna was catalyzed by rna polymerase ii, and the majority of the spliced leader rnas synthesized in vitro ... | 1990 | 2300557 |
| acute topical steroid administration blocks mast cell increase and the late asthmatic response of the canine peripheral airways. | previous studies indicated that mast cell number increased after airway exposure to ascaris suum antigen (ag). the following two series of experiments were performed to test the hypothesis that this phenomenon may be associated with the ag-induced late-phase bronchoconstriction (lar). in the first, two bronchoscopes wedged in airway segments of two contralateral lobes of 16 dogs were used to deliver 0.26, 2.6, or 26 micrograms of ag to one lobe; the other served as a control. after the observati ... | 1990 | 2301857 |
| monoclonal antibody to 2-amino-3-methylimidazo(4,5-f)quinoline, a dietary carcinogen. | in order to investigate relationships between human carcinogenesis and dietary carcinogens, one hybridoma cell line secreting a monoclonal antibody against 2-amino-3-methylimidazo(4,5-f)quinoline (iq), a dietary carcinogen, was produced by fusing splenocytes from balb/c mice immunized with iq-lysine(lys)-ascaris protein conjugate. the subclass of monoclonal anti-iq antibody was determined by double immunodiffusion using culture medium and identified as igg1. monoclonal anti-iq antibody was purif ... | 1990 | 2301946 |
| penetrating the conjunctival barrier. the role of molecular weight. | dinitrophenyl (dnp) derivatives of various molecular weights were tested for their ability to elicit ocular anaphylaxis after topical application to the eye of immunized animals. adult male sprague-dawley rats were immunized by intraperitoneal injection of dnp-ascaris suum extracts and alum and were then skin-tested with dnp-bovine serum albumin on day 13 post-immunization to assess their sensitivity to the dnp hapten. on day 14, animals were challenged topically with dnp derivatives in one eye; ... | 1990 | 2303328 |
| capsaicin and histamine antagonist-sensitive mechanisms in the immediate allergic reaction of pig airways. | the airway vascular and bronchial responses were studied in pigs sensitized with ascaris suum. ascaris, histamine (h) and capsaicin aerosol all induced a clear-cut increase in blood flow in the nasal, laryngeal and bronchial circulation with a decrease in vascular resistance of 20-40%. when delivered to the lung both ascaris and histamine, but not capsaicin, caused pulmonary airflow obstruction with increase in resistance and a fall in dynamic compliance of 40-70%. after pretreatment of pigs wit ... | 1990 | 2309568 |
| inhibitory effect of renal nerve activity during canine anaphylactic hypotension. | we have examined baroreceptor regulation of renal nerve activity (rna) during anaphylactic hypotension that affects renal nerve activity. in anesthetized dogs, mean blood pressure (mbp), heart rate (hr), and rna were simultaneously measured. to test for a normally functioning baroreceptor reflex in each animal, a transient hypotension was induced by an intravenous injection of sodium nitroprusside (4 micrograms/kg). nitroprusside produced a reflex increase in rna (+63 +/- 12% at -20 mmhg and +13 ... | 1990 | 2309932 |
| in vitro induction of crawling in the amoeboid sperm of the nematode parasite, ascaris suum. | in a highly synchronous process, the immotile spermatids of ascaris suum extend pseudopods and become rapidly crawling sperm when treated with an extract from the glandular vas deferens of the male under strict anaerobic conditions. within 9-12 min, a pseudopod develops, elongates rapidly, and exhibits a continuous flow of membrane specializations, the villipodia, from tip toward base. when attached to acid-washed glass, the pseudopod pulls the cell body along at speeds exceeding 70 microns/min. ... | 1990 | 2311127 |
| evaluation of pyrantel-tartrate abbreviated ascaris suum infections for the development of resistance in young pigs against migrating larvae. | crossbred young pigs were used to test whether abbreviated infections with eggs of ascaris suum can stimulate the acquisition of resistance to challenge. weanling pigs from an ascaris-free colony were kept free of a. suum until they were divided into groups at the age of 7-8 weeks. the experimental animals received pyrantel tartrate during the period when they were being exposed to increasing numbers of infective a. suum eggs and challenged 10 days after the last infective dose. liver milk-spot ... | 1990 | 2312230 |
| putrescine n-acetyltransferase in onchocerca volvulus and ascaris suum, an enzyme which is involved in polyamine degradation and release of n-acetylputrescine. | a novel type of n-acetyltransferase, clearly different from the nuclear and cytosolic polyamine n-acetyltransferases of mammals, was recently found in the intestinal nematode ascaris suum. the occurrence of this putrescine n-acetylating enzyme has also been noted in the filarial parasite onchocerca volvulus. the enzyme was partially purified from adults of o. volvulus and a. suum by chromatography on deae-cellulose and cadaverine-sepharose. substrate specificities of the filarial enzyme resemble ... | 1990 | 2320051 |
| sequential resonance assignment and secondary structure determination of the ascaris trypsin inhibitor, a member of a novel class of proteinase inhibitors. | the solution conformation of the ascaris trypsin inhibitor, a member of a novel class of proteinase inhibitors, has been investigated by nuclear magnetic resonance spectroscopy. complete sequence-specific assignments of the 1h nmr spectrum have been obtained by using a number of two-dimensional techniques for identifying through-bond and through-space (less than 5-a) connectivities. elements of regular secondary structure have been identified on the basis of a qualitative interpretation of the n ... | 1990 | 2322539 |
| visualization of chitin-protein layer formation in ascaris lumbricoides egg-shells. | the formation of chitin-protein layer in ascaris lumbricoides egg-shells was studied using the method of chitin structures visualization by means of derivatives of stilbene-disulfonic acid (blankophor, bayer, frg). intensive formation of chitin structures in a. lumbricoides egg-shell occurred only in fertilized females in a very short portion of uterus from the site of connection of the oviduct with the uterus up to 25-30 mm distally, i.e. in the section forming about one fifth or less than one ... | 1990 | 2332201 |
| childhood pica. some aspects of the clinical profile in manchester, jamaica. | the clinical profile of 108 children aged 1 1/2 years to 10 years who practised pica was compared to that of 50 non-pica patients aged 2 years to 10 years. of the patients with pica, 85% were less than 5 years of age and 29% were aged 1 1/2 years to 2 years. the male to female ratio was 1:1.4. ninety-two patients ingested dirt, 35 marl, 33 stone, 17 coal and 10 ashes. twenty-two patients ingested multiple substances. family history for pica was positive in 44 patients. forty-six per cent of pica ... | 1990 | 2333694 |
| structural studies on three flavin-interacting regions of the flavoprotein subunit of complex ii in ascaris suum mitochondria. | the flavoprotein (fp) subunit of mitochondrial complex ii contains covalently bound fad as a prosthetic group. in this study, the primary structure of the flavin-bound tryptic peptide from the fp subunit of ascaris complex ii was determined and found to be highly similar to those of the corresponding flavin-binding regions of bovine heart and bacterial fp subunits. furthermore, the ascaris fp subunit was shown to contain two regions exhibiting striking sequence similarity to the segments that ha ... | 1990 | 2335235 |
| n-terminal amino acid sequence identity between a major allergen of ascaris lumbricoides and ascaris suum, and mhc-restricted ige responses to it. | a protein allergen of the parasitic nematode ascaris has been purified to homogeneity by immunoaffinity chromatography. it is the most abundant protein species in the parasite's body fluid and has been named aba-1. the allergen's molecular weight (mw) has been previously estimated at 14,000, but this sizing is currently under re-evaluation. the immunological activity of the protein was intact after purification, as attested by immunoprecipitation and passive cutaneous anaphylaxis. the ige respon ... | 1990 | 2335378 |
| experimental pulmonary eosinophilia in mice by ascaris suum extract. | the transnasal administration of an extract of the parasite ascaris suum (asc) to c57bl/6 mice for 3 wk produced marked eosinophilia in the bronchoalveolar lavage (bal) fluid. this pulmonary eosinophilia was not accompanied by blood eosinophilia. the oral administration of cyclosporin, 50 mg/kg body weight, every other day significantly suppressed the pulmonary eosinophilia. athymic c57bl/6-nu/nu mice failed to develop pulmonary eosinophilia. these data indicate that pulmonary eosinophilia cause ... | 1990 | 2339848 |
| [determination of circulating filarial antigen using monoclonal antibody against brugia malayi adult worm es antigen with dot-elisa]. | a hybridoma cell line secreting monoclonal antibody (mcab) against brugia malayi adult worm excretory-secretory antigen (awes) was established. the identification of the ig sub-classes showed that mcab awes belongs to igg. we developed a dot-enzyme-linked immunosorbent assay (dot-elisa) to detect circulating parasite antigens in human lymphatic filariasis. 75 of 77 (97.4%) bancroftian microfilaremia cases showed positive reaction. 27 out of 40 (67.5%) microfilarial patients with hydroceles, chyl ... | 1990 | 2340764 |
| neuropeptide diversity in ascaris: an immunocytochemical study. | an immunocytochemical method was used for localization of various peptide-like substances in the ascaris nervous system. out of 45 antipeptide antisera, 12 demonstrated immunoreactivity in different subsets of neurons; these 12 antisera were raised against luteinizing hormone-releasing hormone (lhrh), aplysia peptide l11 (l11), aplysia peptide 12b (12b), small cardioactive peptide b (scpb), neuropeptide y (npy), fmrfamide, gastrin-17, cholecystokinin octapeptide (cck-8), alpha-melanocyte stimula ... | 1990 | 2341616 |
| churg-strauss vasculitis and ascaris infection. | a patient with churg-strauss vasculitis presenting with mononeuritis multiplex, who developed obstructive jaundice, is described. on investigation the jaundice proved to be due to ascaris infestation. as the immune abnormalities associated with ascaris infection are also typical of those seen in the churg-strauss syndrome it is speculated that the vasculitis occurred because of a failure to regulate the anti-ascaris immune response. | 1990 | 2344213 |
| measurement of ascaris lumbricoides infection intensity and the dynamics of expulsion following treatment with mebendazole. | intensity of ascaris lumbricoides infection was measured in terms of egg counts and worm burden in children 2-10 years of age. the expulsion of a. lumbricoides with a 3-day treatment of mebendazole occurred over 8 days, beginning on the second day of treatment. ninety-seven percent of the worms were expelled between the second and seventh days. a rapid means of estimating eggs per gram (epg) by the kato katz technique correlated well with the method described by martin & beaver (1968). in spite ... | 1990 | 2345663 |
| epidemiology and immunology of necator americanus infection in a community in papua new guinea: humoral responses to excretory-secretory and cuticular collagen antigens. | baseline data from an immuno-epidemiological study of hookworm infection in a rural village in madang province, papua new guinea are reported. necator americanus was found to be the commonest helminth infection, with a prevalence of near 100% and intensity of 40 worms per host in adults. enterobius vermicularis, ascaris lumbricoides and trichuris trichiura were also present, at prevalences of 53, 10 and 3% respectively; ancylostoma duodenale was absent. the frequency distribution of n. americanu ... | 1990 | 2345664 |
| control of geohelminths by delivery of targeted chemotherapy through schools. | this study describes the age-targeted chemotherapeutic control of geohelminthiasis in the total population (n = 11,500) of the island of montserrat, west indies. the intervention programme involved the treatment with single dose albendazole of all children aged 2-15 years (approximately 2500) in 4 sequential cycles at intervals of 4 months. infection status was monitored by an initial coprological survey of an age-stratified sample (11.5%) of the population, and by surveys of smaller samples (4- ... | 1990 | 2345909 |
| transcription of a nematode trans-spliced leader rna requires internal elements for both initiation and 3' end-formation. | we have used block substitution mutagenesis and in vitro transcription to define sequence elements important for efficient initiation and 3' end-formation of the trans-spliced leader rna (sl rna) of the parasitic nematode ascaris lumbricoides. these experiments indicate that the sl rna has an unusual promoter structure containing elements which include the 22 nt trans-spliced leader exon itself. efficient transcription is correlated with the binding of a factor to the 22 nt (sl) sequence; mutati ... | 1990 | 2347310 |
| [current status and trends in quality control in parasitology and mycology]. | the survey of the results of the control quality in parasitology and mycology showed in general an improvement in the correct diagnosis for the specimen analysed (preparations of parasites, smears, stools, sera, fungal cultures). the wrong diagnosis of ascaris lumbricoides eggs in stools diminished from 5% to 1.5%. the trichrome gomori-wheatley stain technic on smears in pva was introduced. 16 reference or national standards sera for the parasitological serology, including 6 for toxoplasmosis an ... | 1990 | 2350842 |
| acquired resistance to migrating larvae of ascaris suum in young pigs by repeated drug-abbreviated infections. | cross-bred 3- and 8-wk-old pigs were used to test whether drug-abbreviated infections with ascaris suum can stimulate acquired resistance to challenge. during the immunization period, both age groups of animals were infected with increasing numbers of a. suum eggs (500, 1,000, 2,000, 5,000, 10,000, and 20,000) at 7-day intervals while the pigs were receiving pyrantel tartrate in the feed. two days after the last infective dose, animals were placed on unmedicated feed for 8 days and then challeng ... | 1990 | 2352069 |
| the specificity of the antibody response to internal antigens of ascaris: heterogeneity in infected humans, and mhc (h-2) control of the repertoire in mice. | children from an area of africa endemic for the large roundworm of humans, ascaris lumbricoides, were found to vary considerably in the specificity of their serum igg response to the internal antigens of the parasite. this was particularly noticeable for responses to a 14-kd protein (aba-1) of the parasite that has previously been shown to be the subject of a strong ige antibody response in infected animals. the possibility that this heterogeneity in immune repertoire has a genetic basis was exp ... | 1990 | 2357848 |
| intestinal parasites in central american immigrants in the united states. | we describe the results of ova and parasite examinations of 216 central americans who were seen at a los angeles, calif, clinic during an 8-month period. among the 125 immigrants from central america, intestinal parasitic prevalence was 53%. pathogens were found in 45% and multiple pathogens in 21%. of the 91 us born central american children, parasite prevalence was 14%. pathogens were found in 12%. the most common pathogens were trichuris trichiura, giardia lamblia, and ascaris lumbricoides. g ... | 1990 | 2369250 |
| pulcherosine, a novel tyrosine-derived, trivalent cross-linking amino acid from the fertilization envelope of sea urchin embryo. | the normally hardened and aminotriazole-induced soft fertilization envelopes (fes) of the sea urchin hemicentrotus pulcherrimus and two other species were isolated and investigated for component proteins and cross-linking amino acids. from the acid hydrolysate of the hard fe of h. pulcherrimus, we isolated by reversed-phase high-performance liquid chromatography a novel fluorescent compound as well as dityrosine and trityrosine, the major tyrosine-derived cross-linking amino acids. these three c ... | 1990 | 2372536 |
| [biliary ascariasis as complication after cholecystectomy for cholelithiasis]. | a case history of a 39-year-old turkish female with gallstones is described. cholecystectomy and choledochotomy were performed. in the postoperative period, the patients was found to have an ascaris lumbricoides infection. the epidemiology, complications and diagnostics are briefly reviewed. | 1990 | 2374628 |
| elisa detection of o. volvulus antigenemia in snip-positive patients by monoclonal antibodies directed against a 56 kd glycoprotein of ascaris suum. | | 1990 | 2378214 |
| an integrated system for the control of the major human helminth parasites. | disease due to virtually all of the human helminth parasites can be prevented simultaneously by a simple system based on the following premises: 1) helminths do not multiply within their definitive hosts, and their distribution in host populations is overdispersed; 2) disease manifestations occur largely in the small proportion of hosts, often school children, with heavy worm burdens; and 3) a few single-dose, broad-spectrum anthelmintics given in low doses at prolonged intervals can maintain wo ... | 1990 | 2378224 |
| impact of acute diarrhoea on parasite loads. | parasites and diarrhoea are more frequent in poor populations. parasite prevalence rates in post-diarrhoeal patients of the international centre for diarrhoeal disease research (icddrb) and in non-diarrhoeal populations of two dhaka poor socioeconomic communities have been compared to explore if there is any effect of acute diarrhoea on parasite prevalence rates. stool of a 4% systemic randomly selected sample of diarrhoeal patients and the whole population of two local poor communities have bee ... | 1990 | 2382095 |
| tumor necrosis factor-like cytotoxicity and anorexia in ascaris suum infected pigs. | tumor necrosis factor-like cytotoxicity (tnf-lc) was examined in sera from 12 pigs experimentally infected with ascaris suum. the difference of tnf-lc levels between eight infected and four uninfected controls was not significant. when an endotoxin challenge was intravenously administered 1 month after the first dose of a. suum, the levels of tnf-lc in the sera of infected pigs were one-third that of the controls 125 min post-challenge (pc). in a more detailed study on four infected and two unin ... | 1990 | 2382388 |
| ozone increases susceptibility to antigen inhalation in allergic dogs. | to determine whether o3 exposure increased airway responsiveness to antigen inhalation, we studied airway responsiveness to acetylcholine (ach) and ascaris suum antigen (aa) before and after o3 in dogs both sensitive and insensitive to aa. airway responsiveness was assessed by determining the provocative concentration of ach and aa aerosols that increased respiratory resistance (rrs) to twice the base-line value. o3 (3 parts per million) increased airway responsiveness to ach in dogs both sensit ... | 1990 | 2384406 |
| biotin as a probe of the surface of ascaris suum developmental stages. | sulfo-nhs-biotin (aqueous soluble) and nhs-biotin (organic soluble) labeled similar sds-2me (sodium dodecyl sulfate/beta-mercaptoethanol) soluble cuticular proteins of second stage larvae (l2) and third stage ascaris suum larvae (l3). comparable analysis of biotin-labeled fourth stage larvae (l4), young adults, and mature adult ascaris suum revealed strong labeling of several sds-2me soluble cuticular proteins with nhs-biotin, while sulfo-nhs-biotin appeared to strongly label a single sds-2me so ... | 1990 | 2385267 |
| [analysis of antigens defined by anti-anisakis larvae antibodies of ige and igg type in the sera of patients with acute gastrointestinal anisakiasis]. | using sds-page and immunoblotting assay, we analysed antigens defined by anti-anisakis larvae antibodies of ige and igg type in the sera of patients with acute gastrointestinal anisakiasis. 1) humoral ige response to 76, 64 and 60 kda molecular weight proteins of anisakis larvae and igg response to 91, 76, 64 and 60 kda molecular weight proteins were demonstrated in the sera of patients with acute gastric anisakiasis. those humoral antibody responses were also demonstrated in the sera of two pat ... | 1990 | 2388423 |
| predisposition of individuals and families in mexico to heavy infection with ascaris lumbricoides and trichuris trichiura. | stool specimens from 90% of the population of 2 marginal communities in coatzacoalcos, mexico, were examined by the kato-katz technique for the estimation of eggs per gram of faeces (epg). average epg values for ascaris lumbricoides and trichuris trichiura were calculated for each family in the community, based on the arithmetic mean epg of family members for each parasite. 39 families were classified as either lightly or heavily infected with a. lumbricoides and infected members were treated wi ... | 1990 | 2389320 |
| improvements in physical fitness of kenyan schoolboys infected with hookworm, trichuris trichiura and ascaris lumbricoides following a single dose of albendazole. | we studied physical fitness with the harvard step test (hst), in primary schoolboys infected with hookworm (91% baseline prevalence), trichuris trichiura (94%) and ascaris lumbricoides (39-40%) who received a single 400 mg dose of albendazole or an identical placebo. boys were examined, allocated at random to placebo or albendazole groups, treated, and re-examined 7 weeks later. the 2 groups did not differ significantly before treatment in age, anthropometry, haemoglobin levels, prevalence or in ... | 1990 | 2389321 |
| unusual transcription termination of the ribosomal rna genes in ascaris lumbricoides. | we studied termination of transcription of the ribosomal rna genes in ascaris lumbricoides, the first representative in the phylum of nemathelminthes analysed so far. rnase protection experiments in vivo reveal that the 3' end of the precursor rrna coincides with the end of mature 26s rrna. promoter-containing miniplasmids are able to direct unique 3' end formation in vitro at a site identical to that observed in vivo, whereas deletion of these sequences abolishes 3' end formation throughout the ... | 1990 | 2390973 |
| diagnosis of opisthorchiasis by enzyme-linked immunosorbent assay using partially purified antigens. | opisthorchis viverrini antigens were partially purified from adult worms collected from liver and extrahepatic biliary system of infected hamsters. tegument fraction was obtained by chemical extraction, whereas other fractions were purified by sephadex g-200 gel filtration chromatography. five fractions of o. viverrini antigens were obtained, namely tegument extract, somatic extract, fraction 1 (p1), fraction 2 (p2) and fraction 3 (p3), respectively. the enzyme-linked immunosorbent assay techniq ... | 1990 | 2393450 |
| biophysical transport properties of the cuticle of ascaris suum. | the transport properties of isolated cuticle from ascaris suum were studied using standard two-chamber diffusion cells and a number of radiolabeled permeants which varied in molecular size, lipophilicity and electrical charge. the permeability coefficient of the collagen matrix (lipid-extracted cuticle) vs. molecular radius relationship showed the interdependence of molecular size and electrical charge of the permeants with respect to the aqueous pores of the negatively charged matrix. the perme ... | 1990 | 2398915 |
| chronic pancreatitis in china: etiology and management. | the incidence of chronic pancreatitis in china is unknown. endoscopic retrograde cholangiopancreatography (ercp), surgery, and autopsy demonstrated that chronic pancreatitis in china is mainly secondary to cholelithiasis and other diseases of the bile ducts. stones in the common bile duct, both intra- and extrahepatic, are extremely frequent in china. such patients may have scores, even hundreds, of stones filling the common bile duct and its radicles. biliary tract disease constitutes the etiol ... | 1990 | 2407036 |
| [pulmonary mycoses and parasitosis]. | lungs can be the target of many fungal parasitic organisms. some of them are specific to the lung (pneumocystis); while others will migrate there in the course of their life cycle (ascaris) or be caught in it (paragonimus, echinococcus). sometimes, they also generate hypersensitivity reactions (fungi). in making a diagnosis one has to consider the present state of health or the disease history of the patient. a great number of parasites or mycetes are opportunist agents found in immuno-compromis ... | 1990 | 2407160 |
| [the use of methylene blue-eosin-borax stain in determining the viability of ascaris ova]. | | 1985 | 2408784 |
| sympathetic modulation of biochemical and physiological response to immune degranulation in canine bronchial airways in vivo. | the effect of sympathetic stimulation on bronchial smooth muscle contractile response after mast cell degranulation with ascaris suum antigen was studied in 36 natively allergic dogs in situ. bronchial smooth muscle response was measured isometrically in a single right middle lobe bronchus. a dose of antigen causing maximal release of mediator was administered to the bronchus through the bronchial arterial circulation. serial plasma histamine concentrations were determined at 15-s intervals afte ... | 1985 | 2409114 |
| inhibitory effect of tritoqualine (trq) on histamine release from mast cells. | tritoqualine (trq), used clinically as an antiallergic drug, did not inhibit histidine decarboxylase activity (hdc, ec. 4.1.1.22.) partially purified from fetal rats and the enzymes prepared from mastocytoma p-815 cells. however, trq inhibited the histamine release from rat peritoneal mast cells induced by compound 48/80 and atp. trq was also effective in inhibiting antigen-induced histamine release in rat mast cells sensitized actively or passively by the homologous anti-dnp-ascaris antibody. p ... | 1985 | 2411982 |
| histamine release induced from mast cells by active components of compound 48/80. | compound 48/80 and 14c-labeled compound 48/80 were synthesized, and fractionated by thin-layer chromatography into 14 components (a-n) with various histamine releasing activities and different ca2+ requirements for their actions. the histamine release induced from rat mast cells in vitro by the most active component, fraction d (molecular weight = 2280, a tridecamer composed of 13 monomer units), was greatly enhanced by extracellular ca2+, and was partially reduced by pretreatment of the cells w ... | 1985 | 2415369 |
| binding of active components of compound 48/80 to rat peritoneal mast cells. | the binding characteristics of compound 48/80 were examined using rat mast cells and fractionated 14c-labeled compound 48/80 components at 4 degrees c in vitro where no degranulation of the cells occurred. the binding potencies of these components in the presence of ca2+ generally paralleled their histamine releasing activities, except in the case of fractions g (decamer) and h (nonamer), both ca2+-independent releasers, for the binding of which ca2+ was inhibitory. scatchard analyses and displa ... | 1985 | 2415370 |
| augmentation of respiratory mast cell secretion of histamine caused by vagus nerve stimulation during antigen challenge. | we studied the effect of vagus nerve stimulation on the mast cell secretion of histamine after intraarterial (i.a.) administration of ascaris suum antigen (aa) into the bronchial circulation of 10 randomly selected, natively allergic dogs in vivo. respiratory mast cell response was measured as the arteriovenous difference (avd) in histamine concentration across the bronchus. plasma histamine concentration was determined simultaneously from right atrium, right ventricle, and femoral artery 60 and ... | 1986 | 2416826 |
| isolation of specific antigens from angiostrongylus cantonensis. 1. preparative flatbed isoelectric focusing. | electrophoresis on sds gel and analytical isoelectric focusing showed that a crude extract of angiostrongylus cantonensis consisted of at least 40 protein components with molecular weights ranging from 13 000-70 000 and isoelectric points of pi values ranging from 3.7-10.0. crossed-immunoelectrophoresis with a hyperimmune antiserum to a. cantonensis showed at least 40 different antigenic components in the crude worm extract which were cross-reactive with those of ascaris suum, metastrongylus apr ... | 1985 | 2417425 |
| characterization of fpl-52694 [5-(2-hydroxypropoxyl)-8-propyl-4-oxo-4h-benzopyran-2-carboxylic acid na] on histamine release from rat peritoneal mast cells induced by antigen, compound 48/80 and a 23187. | we studied the in vitro effects of fpl-52694 [5-(2-hydroxypropoxyl)-8-propyl-4-oxo-4h-benzopyran-2-carboxylic acid na] on histamine release from rat peritoneal mast cells. these cells exposed to ascaris antigen, compound 48/80 or the ionophore a 23187 concentration-dependently released histamine. about a 30-40% histamine release was obtained by 1 x 10(-4) g/ml of antigen, 1 x 10(-7) g/ml of compound 48/80 and a 23187. fpl-52694 (10(-9)-10(-4) g/ml) concentration-dependently inhibited the histami ... | 1985 | 2417456 |
| purification of ascaris suum antigen: its allergenic activity in vitro and in vivo. | crude aqueous extracts of ascaris suum (ce) have been used widely to study ige-mediated reactions in various experimental preparations. because some ce may contain a polypeptide, a mast cell degranulating peptide (mcdp), that degranulates mast cells by nonimmunologic mechanisms, various protocols have been used to ensure that the ascaris preparation used did not contain mcpd. in general, these protocols have assumed mcdp had been without providing proof. even protocols designed to isolate the ma ... | 1986 | 2419382 |
| increased metachromatic cells and lymphocytes in bronchoalveolar lavage fluid of dogs with airway hyperreactivity. | bronchoalveolar lavage (bal) was performed in 11 basenji greyhound (bg) dogs, which showed persistent airway hyperreactivity to methacholine and citric acid aerosols, and in 15 non-bg dogs, which were significantly less reactive to these challenges. five of the bg dogs had never received any aerosols prior to bal, and 3 of the non-bg dogs were allergic to ascaris suum. no dog received aerosols for 2 wk prior to bal. fluid recovered was centrifuged, and aliquots were taken for histamine content a ... | 1986 | 2420243 |
| studies on bronchial hyperreactivity, allergic responsiveness, and asthma in rural and urban children of the highlands of papua new guinea. | the prevalence of asthma and allergic responsiveness in rural and urban children of the highlands of papua new guinea was studied. bronchial provocation studies with histamine demonstrated significant bronchial hyperreactivity in 0.5% (1 in 195) rural and 1.7% (1 in 59) urban children, rates which were significantly lower than those observed in corresponding adult populations (7%). urban children demonstrated a higher incidence of skin test reactivity toward dermatophagoides pteronyssinus, asper ... | 1986 | 2420852 |
| ascaris lumbricoides: monoclonal antibodies against the major cuticular collagen. | | 1986 | 2423361 |
| blocking of nonspecific igg in indirect radioimmunoassay for detecting ascaris suum antigens. | the sensitivity of an indirect radioimmunoassay (iria) used for detecting larval body wall (lbw) antigens of ascaris suum was enhanced by using various blocking agents which prevented nonspecific binding of immunoglobulin g (igg) or free 125i without preventing binding of specific antibodies to the antigen. the use of blocking agents reduced counts for both positive and negative sera, resulting in an increase in calculated binding ratios (br) and deltas and, thus, in the sensitivity of the assay ... | 1986 | 2424166 |
| exploratory and confirmatory plots of single-channel records. | graphical displays are used to study open- and closed-time durations of a single ion-channel. cusum plots, in conjunction with kolmogorov-smirnov type tests, provide checks for changes in channel behaviour. distributional properties are examined by means of q-q and p-p plots. the methods are illustrated using gaba-activated channel records from ascaris suum. | 1986 | 2425195 |
| inhibitory mechanism of tritoqualine on histamine release from mast cells. | tritoqualine (trq, (+)-(r*)-7-amino-4,5,6-triethoxy-3-[(r*)-5,6,7, 8-tetrahydro-4-methoxy-6-methyl-1,3-dioxolo[4,5-g]isoquinolin++ +-5-yl] phthalide) strongly inhibited the increased metabolism of [3h]arachidonic acid-labeled phospholipid and 45ca2+ influx in mast cells stimulated by compound 48/80 (compd 48/80), concanavalin a (con a) plus phosphatidylserine (ps), or 2,4-dinitrophenyl-coupled-ascaris extracts (dnp-asc). however, trq did not disturb the binding of 14c-labeled compd 48/80 to the ... | 1986 | 2428378 |
| the pharmacology of nedocromil sodium. | the pathophysiology of obstructive airways disease involves, in varying degree, components of reversible bronchoconstriction, inflammation and bronchial hyperreactivity. this multifactorial aetiology is the probable reason why no single animal model exists which is predictive of therapeutic efficacy in airway diseases, including asthma. nedocromil sodium has therefore been profiled in a number of systems ranging from simple in vitro tests and in vivo models of passive anaphylaxis to complex mode ... | 1986 | 2429854 |
| integumentomuscular sac of ascaris lumbricoides suis l: a biomembrane for studies on the transport of chemical substances. | the whole integumentomuscular sac of the worm ascaris lumbricoides suis l. and pouches isolated from it seemed to be a good model for investigation of the transmembrane transport of chemical substances across tissue barriers. | 1986 | 2430266 |
| central role of cyclooxygenase in the response of canine peripheral airways to antigen. | we studied the effects of antigen aerosol challenge on the airways of the canine peripheral lung and examined the roles of cyclooxygenase products, histamine, and cholinergic activity in the responses. one-minute deliveries of 1:10,000 or 1:100,000 concentrations of ascaris suum antigen aerosol through a wedged bronchoscope resulted in mean maximal increases in collateral system resistance (rcs) of 415 and 177%, respectively, after 4-8 min. repeated antigen challenge (1:100,000) resulted in sign ... | 1986 | 2430928 |
| characterization of primate bronchoalveolar mast cells. i. ige-dependent release of histamine, leukotrienes, and prostaglandins. | large numbers of functional mast cells were obtained by bronchoalveolar lavage (bal) of macaca arctoides monkeys that had been infected with the nematode ascaris suum. these lavage cells, of which 21% were mast cells, released histamine, ltc4, and pgd2 in a concentration-dependent fashion when challenged with ascaris antigen or antibody to human ige. however, there was no release of histamine when these cells were challenged with compound 48/80. the amount of mediator released was highly depende ... | 1986 | 2431048 |
| characterization of primate bronchoalveolar mast cells. ii. inhibition of histamine, ltc4, and pgd2 release from primate bronchoalveolar mast cells and a comparison with rat peritoneal mast cells. | as described in the preceding companion paper, bronchoalveolar lavage (bal) of the primate macaca arctoides infected with the nematode ascaris suum yields a population of cells containing a high proportion of mast cells (21%). nedocromil sodium, a new drug undergoing clinical evaluation for the treatment of reversible obstructive airways disease, inhibited the release of histamine, ltc4, and pgd2 from these cells challenged with antigen (with ic30 values of 2.1 x 10(-6) m, 2.3 x 10(-6) m, and 1. ... | 1986 | 2431049 |
| inhibitory action of verapamil and chlorpromazine on the release of slow-reacting substance of anaphylaxis from cat lung. | verapamil, a calcium channel blocker, significantly inhibited the release of slow-reacting substance of anaphylaxis (srs-a) from cat lung fragments challenged with either the calcium ionophore, a23187, or ascaris extract. this effect was observed when verapamil was present at the concentrations of 10 microm and 50 microm. chlorpromazine 10 microm significantly inhibited antigen-induced srs-a release. histamine release was low and no significant effect by verapamil or chlorpromazine could be show ... | 1986 | 2432620 |
| [fixation of early ascarid embryos for electron microscopic research]. | ascaris embryos represent a classical object in embryology, but their ultrastructure has not been so far studied because their sheets are not permeable to fixatives used for electron microscopy. thus, the fixation with glutaraldehyde for 24 hours followed by a 24 hour oso4-fixation led to osmification of 1% embryos. the osmification of 100% embryos was achieved after addition of detergents, sodium dodecylsulfate or triton x-100 to aldehyde fixative. the best fixation was povided using sodium dod ... | 1986 | 2433823 |
| effects of the new antiallergic drug 11-oxo-11h-pyrido[2,1-b] quinazoline-2-carboxylic acid on bronchial and cutaneous allergic responses to ascaris in dogs. | the effects of 11-oxo-11h-pyrido[2,1-b]quinazoline-2-carboxylic acid (sm 857), a new antiallergic drug, on both bronchial asthma and active cutaneous anaphylaxis (aca) reaction induced by ascaris suum antigen in dogs were investigated. the airway resistance was determined using the modified konzett-rössler method. sm 857 in doses of 30 and 100 mg/kg intraduodenally (i.d.) produced a remarkable inhibitory effect on the asthmatic bronchoconstriction induced by inhalation of ascaris antigen in natu ... | 1986 | 2434112 |
| antigenic similarity of onchocerca volvulus to other helminths examined by monoclonal antibodies against o. volvulus. | antigens in onchocerca volvulus were characterized using anti-o. vovulus monoclonal antibodies (mcab). a total of 17 mcab which were not species-specific to o. volvulus was examined for the antigenic similarity of o. volvulus with 12 parasitic helminths using indirect immunofluorescence. the range of reactivity of these mcab was wider in nematodes in contrast to species of cestoda or trematoda. the antigenic similarity within helminths examined was generally in agreement with their taxonomic rel ... | 1986 | 2434696 |
| a high-conductance calcium-dependent chloride channel in ascaris suum muscle. | the properties of a high-conductance ca-dependent cl channel in the body muscle membrane of the nematode parasite ascaris suum were examined with the patch-clamp technique. in ascaris ringer solution the current-voltage relationships of the channel appeared linear during hyperpolarization with a slope conductance of 200 ps (in symmetrical 175 mm-cl): during depolarization the slope conductance reduced. cation and anion replacement experiments showed this channel to be permeable to cl ions. in ar ... | 1987 | 2436251 |
| the effect of trans-4-guanidinomethylcyclohexanecarboxylic acid p-tert-butylphenyl ester hydrochloride (nco-650) on ascaris suum antigen-induced bronchoconstriction in dogs. | antiallergic asthma effect of trans-4-guanidinomethylcyclohexane-carboxylic acid p-tert-butylphenyl ester hydrochloride (nco-650), a new anti-allergic drug, was investigated in comparison with those of tranilast and disodium cromoglycate (dscg) in anesthetized dogs. the asthmatic bronchoconstriction was induced by inhalation of ascaris suum antigen (asc-ag) to naturally ascaris-sensitive dogs. the airway resistance was determined using the modified konzett-rössler method. both intravenous (1 and ... | 1987 | 2437348 |
| ci-922--a novel, potent antiallergic compound--ii. activity in animal models of allergy. | ci-922 (3,7-dimethoxy-4-phenyl-n-1h-tetrazol-5-yl-4h-furo [3,2-b]indole-2-carboxamide, l-arginine salt) is an effective antiallergic in guinea pig, rat, and canine models. this in vivo activity is attributed to its ability to inhibit inflammatory mediator release following antigen challenge in these species. antigen-induced anaphylaxis in guinea pigs (collapse), which is mediated predominantly by histamine, was prevented by i.p. drug pretreatment. ci-922 (5 mg/kg, i.v.) reduced i.v. antigen-indu ... | 1987 | 2438239 |
| inhibitory immunization using complete freund's adjuvant on ongoing eosinophilia induced by repeated antigen-stimulations in guinea pigs. | ongoing eosinophilia, in guinea pigs repeatedly sensitized with dinitrophenylated (dnp)-ascaris antigen (dnp-asc) into the peritoneal cavity, was suppressed significantly by complementary immunizations with a heterologous antigen, e.g., ovalbumin (oa) or bovine gamma-globulin (bgg) emulsified in complete freund's adjuvant (cfa). challenge injections of dnp-oa or dnp-bgg elicited peritoneal eosinophilia in animals that were sensitized repeatedly with dnp-asc. under these conditions, antigen speci ... | 1987 | 2439463 |
| studies on the anti-allergic action of n-(3',4'-dimethoxycinnamoyl) anthranilic acid (n-5'). | the effects of the anti-allergic compound (n-(3',4'-dimethoxycinnamoyl) anthranilic acid (n-5' or tranilast) have been investigated upon antigen-induced contractions of respiratory smooth muscle in vitro and bronchoconstriction in vivo. n-5' (3.1 x 10(-5) m) in vitro had no significant effect upon antigen-induced contractions of the guinea pig trachea. however, when tested at concentrations of 9.2 and 3.1 x 10(-5) m, but not 0.9 x 10(-5), n-5' significantly inhibited antigen-induced contractions ... | 1987 | 2441619 |
| granulomatous peritonitis due to ascaris lumbricoides. | a child with granulomatous peritonitis caused by ascariasis is described. laparotomy revealed no obvious evidence of intestinal perforation. it is postulated that a worm escaped through the appendicular tip, causing transient peritonitis followed by adhesions and granulomatas in the peritoneal cavity. | 1987 | 2441652 |
| ca-dependent and ca-independent histamine release from mast cells induced by active components of compound 48/80. | compound 48/80 and 14c-labelled compound 48/80 were synthesized, and fractionated by thin-layer chromatography into 14 components with various histamine-releasing activities and different ca++ requirements for their actions. the histamine release induced from rat mast cells in vitro by the most active component, fraction d (molecular weight = 2280, a tridecamer composed of 13 monomer units), was greatly elevated by extracellular ca++, and was partially reduced by pretreatment of the cells with d ... | 1987 | 2442114 |
| the effects of chlorpheniramine and antiallergic drugs on ascaris suum antigen-induced active cutaneous anaphylaxis in dogs. | effects of chlorpheniramine and two antiallergic drugs on the active cutaneous anaphylaxis (aca) reaction induced by intradermal injection of ascaris suum antigen in naturally sensitized dogs were investigated. chlorpheniramine (10 mg/kg, intraduodenally (i.d.)) almost abolished the aca reaction. nco-650 (100 mg/kg, i.d.) had no inhibitory effect, while tranilast (300 mg/kg, i.d.) showed a weak inhibitory effect. these findings show that the aca reaction is almost totally mediated by histamine a ... | 1987 | 2442443 |
| phosphofructokinase from dirofilaria immitis. stimulation of activity by phosphorylation with cyclic amp-dependent protein kinase. | phosphofructokinase has been partially purified from the filariid helminth, dirofilaria immitis, using ion exchange and affinity chromatography. the d. immitis phosphofructokinase cross-reacted with antibodies prepared against the phosphofructokinase from ascaris suum. these antibodies had been bound to agarose beads. the enzyme was eluted from the immobilized antigen-antibody complex by denaturing agents, and the subunit molecular weight determined by sodium dodecyl sulfate gel electrophoresis ... | 1988 | 2449442 |
| induction of antigen-specific bronchial reactivity to trimellityl-human serum albumin by passive transfer of serum from humans to rhesus monkeys. | a rhesus monkey model was developed to demonstrate the pathogenetic role of ige to chemical hapten-protein conjugates in causing human occupational asthma from reactive chemicals. serum from a worker with trimellitic anhydride (tma) asthma that contained high titers of ige, igg, igm, and iga to trimellityl-human serum albumin (tm-hsa) was aerosolized into the lungs of two monkeys to afford passive airway sensitization. after the monkeys were challenged with aerosolized tm-hsa, pulmonary function ... | 1988 | 2450939 |
| effects of antihistamines on the cardiopulmonary changes due to canine anaphylaxis. | histamine has long been considered to be an important chemical mediator in the pathogenesis of immediate hypersensitivity reactions. we evaluated the efficacy of antihistamines to determine the physiological role of histamine in canine anaphylaxis. either a saline vehicle (control group), an h1 antihistamine (chlorpheniramine, 10 mg/kg), or this h1 antihistamine and an h2 antihistamine (cimetidine, 30 mg/kg) was administered to three separate groups of anesthetized dogs (n = 8). cardiopulmonary ... | 1988 | 2451656 |
| evidence for the absorption and synthesis of 5-hydroxytryptamine in perfused muscle and intestinal tissue and whole worms of adult ascaris suum. | the metabolites of 5-hydroxytryptamine (5-ht, serotonin) namely, l-tryptophan, 5-hydroxytryptophan, 5-hydroxyindole acetic acid and 5-hydroxytryptophol were measured in perfused tissue and whole worms from adult female ascaris suum using reversed-phase liquid chromatography with electrochemical detection. the intracellular levels of each metabolite were quantitated in response to several physiological effectors but only l-tryptophan (trp) caused dose-dependent changes in these metabolites. serot ... | 1988 | 2452401 |
| study of the mechanism of inhibitory action of tranilast on chemical mediator release. | we investigated the mechanism of inhibitory action of tranilast on chemical mediator release by antigen-antibody reactions. tranilast (10(-5)-10(-3) m) inhibited antigen (dnp-ascaris)-induced histamine release from sensitized purified rat mast cells (pmc), but did not show an obvious influence on intracellular cyclic amp. 45ca uptake into pmc induced by antigen (300 micrograms/ml) was obviously suppressed by tranilast (10(-6)-10(-3) m). tranilast (10(-4) m) inhibited antigen-induced histamine re ... | 1988 | 2452912 |
| mechanism of inhibitory action of tranilast on the release of slow reacting substance of anaphylaxis (srs-a) in vitro: effect of tranilast on the release of arachidonic acid and its metabolites. | we investigated the mechanism of inhibitory action of tranilast, one of the anti-allergic drugs, on the release of slow reacting substance of anaphylaxis (srs-a). ionophore a23187 (0.5 or 0.2 micrograms/ml)-induced srs-a release from rat peritoneal exudate cells (pec) or human leucocytes was inhibited by tranilast (10(-5)-10(-3) m). the ic50 (the concentration which gives 50% inhibition) of tranilast on these reactions was approx. 10(-4) m. prostaglandin (pg)e2 release from sensitized purified r ... | 1988 | 2452913 |
| effects of antiallergic drugs on bronchial and cutaneous anaphylaxis in lewis rats. | effects of antiallergic agents on 2,4-dinitrophenylated ascaris extract (dnp-as)-induced bronchial asthma were studied in lewis rats, and compared with those on passive cutaneous anaphylaxis (pca). effects of methysergide and chlorpheniramine on the bronchial asthma model were also investigated. rats were actively sensitized with dnp-as antigen and with killed bordetella pertussis. after 8 d, asthmatic response was provoked by inhalation of dnp-as. the bronchomotor response was measured with a m ... | 1988 | 2454312 |