| [analysis of electrophoretic patterns of three enzymes of oncomelania hupensis]. | allozyme electrophoresis was carried out in starch gel to compare the electrophoretic patterns for acid phosphatase (acp, ec 3. 1. 3.2), peroxidase (po, ec 1.11.1.7) and esterase (est, ec 3. 1. 1. 1). it was found in this study that isoenzymes acp1, acp2 and po1, po2 were coded by 2 loci respectively and they were monomorphic. it is possible to determine 4 loci responsible for esterase zymogram, among which isoenzyme est3 region either consisted of a fast (est3-f) band in the case of oncomelania ... | 1994 | 7720201 |
| [effect of artemether on glycogen, protein, alkaline phosphatase and acid phosphatase of schistosoma japonicum]. | when mice infected with schistosoma japonicum for 32-35 d were treated ig with artemether (art) at a single dose of 300 mg.kg-1 for 24 h, the glycogen content of female and male schistosomes decreased significantly with reduction rates of about 50%. 72 h after medication, the glycogen reduction rates were 64.1-77.9%. meantime, the protein content of female and male worms was also decreased, the reduction rates being 68.1% and 49.3%, respectively. in infected mice treated ig with art at the same ... | 1994 | 7720202 |
| human monoclonal antibodies heterogeneously express a human cross-reactive idiotype associated with immune function in schistosoma japonicum infection. | hybridomas secreting human monoclonal antibodies (hmab) were derived from epstein barr virus (ebv) transformed lymphocytes of a patient with acute schistosoma japonicum infection. three igg1 hmab sj-d, sj-e, and sj-f bind soluble egg antigens (sea) as determined by elisa. these hmab exhibit identical western blot profiles, recognizing an epitope(s) of multiple antigens with apparent molecular weights between 42 and 75 kda. serological analysis of these hmab revealed a heterogeneity in their expr ... | 1994 | 7756581 |
| chemical modification of a cloned glutathione s-transferase from schistosoma japonicum: evidence for an essential histidine residue. | diethylpyrocarbonate (dep) inhibits the catalytic activity of a cloned glutathione s-transferase from schistosoma japonicum (sj26gst) with a second-order rate constant of 474 m-1 min-1 at ph 7.0 and 25 degrees c. there is an accompanying increase in absorbance at 242 nm due to the formation of n-carbethoxyhistidyl derivatives. there was no evidence that tyrosine or cysteine residues were modified by dep treatment nor did the enzyme undergo any major conformational change. activity can be restore ... | 1995 | 7758542 |
| the expression in e. coli and purification of isolated non-thioredoxin-like domains of human pdi. | | 1995 | 7758789 |
| anti-interleukin-4 treatment diminishes secretion of th2 cytokines and inhibits hepatic fibrosis in murine schistosomiasis japonica. | anti-interleukin-4 (il-4) treatment of schistosoma japonicum-infected mice markedly inhibited in vitro secretion of the th2 cytokines il-4 and il-5 from antigen-stimulated spleen cells, but enhanced the secretion of the th1 cytokine ifn-gamma. il-2 secretion was unaffected. hepatic fibrosis was markedly diminished in anti-il-4-treated-mice at ten weeks of infection while granulomas around s. japonicum eggs in the livers were slightly-to-moderately increased in size. the number of eggs per worm p ... | 1995 | 7761107 |
| high level production of hybrid potyvirus-like particles carrying repetitive copies of foreign antigens in escherichia coli. | synthesis in e. coli of native coat protein of johnsongrass mosaic virus, and hybrid protein molecules containing foreign antigens, resulted in the intracellular formation of potyvirus-like particles (pvlps). the foreign antigens used were an octapeptide epitope from plasmodium falciparum and a decapeptide hormone (luteinizing hormone releasing hormone) at the n- or at both n- and c-terminal regions of the coat protein molecule, and a full length protein antigen (sj26-glutathione s-transferase o ... | 1993 | 7764098 |
| production and secretion of recombinant proteins in dictyostelium discoideum. | we have expressed useful amounts of three recombinant proteins in a new eukaryotic host/vector system. the cellular slime mold dictyostelium discoideum efficiently secreted two recombinant products, a soluble form of the normally cell surface associated d. discoideum glycoprotein (psa) and the heterologous protein glutathione-s-transferase (gst) from schistosoma japonicum, while the enzyme beta-glucuronidase (gus) from escherichia coli was cell associated. up to 20mg/l of recombinant psa and 1mg ... | 1994 | 7764951 |
| effect of colchicine on hepatic glycosaminoglycan metabolism in mice infected with schistosoma japonicum. | to study the effect of colchicine on hepatic glycosaminoglycan (gag) metabolism in mice infected with schistosoma japonicum. | 1995 | 7771194 |
| ultrastructural observations on cercaria of schistosoma japonicum. | the present paper describes the ultrastructure of the surface topography, head organ, tegument, musculature, glandular system, primary alimentary tract and flame cells of the chinese mainland strain of schistosoma japonicum cercaria, as visualized with both scanning and transmission electron microscopes. the results not only illustrate the morphological features of the cercarial surface and its internal structure reflecting an adaptation to the aqueous habitat but also reveal the correlation bet ... | 1994 | 7777916 |
| purification of 31/32 kda proteins of adult schistosoma japonicum as antigens (sj 31/32) for elisa and iha. | major diagnostic proteins of 31/32 kda were purified from soluble adult worm homogenate of schistosoma japonicum using aca ultragel chromatography and additional treatments. the purity of the isolated antigenic proteins (sj 31/32) was demonstrated by sds-page and western blotting technique. the antigens showed a negative reaction when stained by pas and remained active after treatment with sodium periodate, moving toward anode in the electrophoretic field. these purified antigens were used for d ... | 1995 | 7788890 |
| [dynamics of il-2 and ifn-gamma levels induced by sea or con a in spleen cells of schistosoma japonicum-infected mice]. | the capacity of mitogen con a and sea-stimulated spleen cells to produce cytokines ifn-gamma and il-2 was studied in s. japonicum-infected mice every two weeks from 0 to 14 wk after infection. the results showed that the levels of these two cytokines began to rise at the 4th wk after infection and reached a peak level at 6-8 wk, then declined to the levels similar to those pre-infection at 12-14 wk after infection. the ifn-gamma level reached the peak earlier than the il-2 level. the dynamics of ... | 1995 | 7788892 |
| scanning electron microscope observation on tegumental alteration of schistosoma japonicum induced by levo- and dextro-praziquantel. | when mice infected with schistosoma japonicum for 35 d were treated ig with levo-praziquantel (l-pra) 150 mg.kg-1 or dextro-praziquantel (d-pra) 150-600 mg.kg-1, the alterations of the worm tegument were examined by scanning electron microscopy within 1-24 h after administration. the results showed that l-pra induced apparent and extensive damage on the worm surface including severe swelling, fusion, erosion and peeling of tegument accompanied by attachment of the host leukocytes. the discoid-li ... | 1995 | 7788895 |
| [pathological changes in the livers of rabbits infected with schistosome cercariae and treated with artemether or praziquantel in the early stage of infection]. | when rabbits infected with schistosoma japonicum cercariae for 7 d were treated ig with artemether (art) 30 mg.kg-1, and repeated at 1-wk intervals (i.e. on d 14, 21, 28 after infection) for 4 times, dead worms were found in the liver sections, but no egg or egg granuloma was detected. in infected rabbits treated repeatedly with the above-mentioned dosage of art at 2-wk intervals (i.e. on d 21, 35, 49) after the first dosing on d 7 after infection, few eggs and egg granulomas were seen occasiona ... | 1995 | 7788896 |
| [observation on antibody levels of rabbits infected with single sex cercariae of schistosoma japonicum]. | nine rabbits infected with male s. japonicum cercariae (worm recovery 15-133, mean 53.1) and 9 rabbits infected with female cercariae (worm recovery 1-102, mean 36.1) were bled periodically until 1 year post-infection. the sera were tested by elisa, la and chr for antibody detection. results showed that the positive rates were 88.9%, 55.6% and 83.3%, respectively. the duration of positive reaction was 3-51 wk post-infection for elisa, 4-17 wk for la and 2-35 wk for chr and the fluctuated results ... | 1995 | 7788897 |
| the 26-kda glutathione-s-transferases from chinese and philippine schistosoma japonicum are identical. | | 1994 | 7810392 |
| immunogenicity of nef protein of sivsmm-pbj14 expressed in a live vaccine strain of salmonella species. | the nef gene of an infectious molecular clone of sivsmm isolate pbj14 was fused to the glutathione s-transferase gene of schistosoma japonicum to generate plasmid pemc100. the recombinant plasmid was placed in an aroa live vaccine salmonella dublin strain, and the production of gst-nef protein was induced by exposure to iptg. the fusion protein was purified and administered as vaccine to balb/c mice by i.p. injection. several doses of the purified fusion protein produced an earlier anti-gst-nef ... | 1994 | 7811532 |
| reinfection with schistosoma japonicum after treatment with praziquantel in poyang lake region, china. | the study on reinfection with schistosoma japonicum after treatment was carried out in a cohort of subjects in a heavy endemic village of poyang lake region, china. after mass treatment with praziquantel in non-transmission time, detailed observations of water contact were estimated using the mean area of skin exposed daily. one year after treatment, the prevalence of infection in study subjects was 54.48%, returning to 83% of initial prevalence. the peak prevalence occurred the 11-15 year age c ... | 1994 | 7825007 |
| evaluation of defined antigen vaccines against schistosoma bovis and s. japonicum in bovines. | our objective is to contribute to the development of defined antigen vaccines for schistosomiasis by evaluating the protective efficacy of schistosoma bovis and s. japonicum antigens in their natural bovine hosts. antigens under evaluation include some already identified as vaccine candidates: glutathione s-transferases (gsts); klh, which shares protective epitopes with the protective antigen gp38 of s. mansoni; and sj23, the analogue of the vaccine candidate sm23 antigen. in another approach, s ... | 1994 | 7825230 |
| schistosomiasis research and the european community. | schistosomiasis research within the framework of the commission of the european communities 'science and technology for development' (cec/std) programme is targeted at three specific problems: diagnosis of infection and disease; the dynamics of transmission, immunity, and morbidity; and the need for improved tools and strategies for control. several important advances have been made over the past decade. improved methods of diagnosis by detection of circulating antigens are in an advanced stage ... | 1994 | 7825231 |
| electron microscopic observations of tegumental damage in adult schistosoma japonicum after in vivo treatment with levo-praziquantel. | the in vivo effects of a single dose of levo-praziquantel, 75 mg/kg in peg 400, on the tegumental surface of adult s. japonicum were compared with the effects of a single dose (150 mg/kg) of the mixed isomer preparation, using scanning and transmission electron microscope. worms were recovered from mice at 10 min, 30 min, 1 hr, 4 hr, 12 hr, 24 hr and 48 hr after treatment. after 10 min exposure to either compound, the tegumental folds and sensory organelles were swollen and the tegument vacuolat ... | 1994 | 7835105 |
| adult schistosoma mansoni express cathepsin l proteinase activity. | this report presents the deduced amino acid sequence of a novel cathepsin l proteinase from schistosoma mansoni, and describes cathepsin l-like activity in extracts of adult schistosomes. using consensus primers specific for cysteine proteinases, gene fragments were amplified from adult s. mansoni cdna by pcr and cloned. one of these fragments showed marked identity to sm31, the cathepsin b cysteine proteinase of adult s. mansoni, whereas another differed from sm31 and was employed as a probe to ... | 1994 | 7838171 |
| crystal structures of a schistosomal drug and vaccine target: glutathione s-transferase from schistosoma japonica and its complex with the leading antischistosomal drug praziquantel. | glutathione s-transferase (gst), an essential detoxification enzyme in parasitic helminths, is a major vaccine target and an attractive drug target against schistosomiasis and other helminthic diseases. crystal structures of the 26 kda gst from the helminth schistosoma japonica (sjgst) have been determined for the unligated enzyme (resolution = 2.4 a, r-factor = 19.7%) and for the enzyme bound to the leading antischistosomal drug praziquantel (resolution = 2.6 a, r-factor = 21.2%). the protein, ... | 1995 | 7853399 |
| strain complex of schistosoma japonicum in the mainland of china. | the present paper deals with studies on the characteristics of schistosoma japonicum isolated from five localities in the mainland of china. the following items were observed and compared including morphometric data, susceptibility of six mammalian hosts, prepatent period, compatibility between larvae and snail hosts, size of hepatic granuloma produced by eggs, immunoreactions in experimental animals, sensitivity to praziquantel, sds-page protein pattern and its antigenicity analysis, dna hybrid ... | 1994 | 7855634 |
| concentration time course of praziquantel in filipinos with mild schistosoma japonicum infection. | despite extensive use of praziquantel, the current drug of choice for the treatment of schistosomiasis and other helminthic infections, little information is available about its pharmacokinetics in individuals living in geographic areas where such infections are endemic. we investigated the pharmacokinetics of praziquantel by determining its serum concentration-time course in four selected filipino volunteers with mild schistosoma japonicum infection who lived in an endemic area in the southern ... | 1994 | 7855670 |
| [study on allele frequency in oncomelania from the mainland of china]. | nine populations of oncomelania, field-collected from anhui, shanghai, jiangsu, zhejiang, jiangxi, hunan, hubei, sichuan and yunnan were studied by horizontal starch gel electrophoretic method with 24 enzyme systems (aat, acph, ak, ao, aph, ck, est, gdh, gpi, g6pd, hbd, isdh, lap, ldh, me, mdh, mpi, nadd, oct, pgm, 6pgd, sdh, sod, xdh) analyzed. 40 loci and 117 alleles were detected in the oncomelania. both of gpi and pgm-i, with 7 alleles, were the most variable loci. 22 loci had more than 3 al ... | 1994 | 7867149 |
| schistosoma japonicum: size of egg granulomas in vaccinated and non-vaccinated hosts as observed in bovines. | the present paper reported the results of studies on the size of schistosoma japonicum egg granulomas in susceptible and poorly susceptible, vaccinated and non-vaccinated, hosts as observed in bovines. it was noted that in both cattle and buffaloes, the mean size of the granuloma was smaller in vaccinated than in the non-vaccinated. the vaccinated bovines will accordingly not only be benefited by the reduction of the survival rate of the challenged schistosomula but also by decreasing the size o ... | 1994 | 7867155 |
| [dynamic study on relationship between serum seaic level and hepatic pathological changes in mice infected with schistosoma japonicum]. | using purified rabbit polyclonal antibodies to sea, avidin-biotin system and capture elisa technique, we observed the dynamic changes in the level of the circulating soluble egg antigen-antibody complex (seaic) in murine sera at various weeks post infection. simultaneously, the diameter and area of liver egg granuloma were measured by using profile analytical technique. serum seaic was first detected 4 weeks post infection (p.i.), reaching peak level at 6-7th week, and then gradually dropped, an ... | 1994 | 7867157 |
| [relationship between the sex of schistosoma japonicum and circulating antigen detection]. | single sex cercariae were used to infect rabbits and the detection of circulating membrane antigen by dot-elisa were followed up until 1 year postinfection. 9 rabbits infected with male cercariae (worm recovery 15-133, mean 53.1) and 9 rabbits infected with female cercariae (worm recovery 1-102, mean 39.1) were examined but no positive reaction was occurred. on the contrary, 7 rabbits infected with bi-sex cercariae (worm recovery 4-161, mean 67.1) all showed positive reactions 6-10 wks post-infe ... | 1994 | 7867161 |
| production and characterization of polyclonal antibodies recognizing the intracytoplasmic third loop of the 5-hydroxytryptamine1a receptor. | the portion of the complementary dna encoding the third intracellular loop of the rat 5-hydroxytryptamine1a (serotonin) receptor was subcloned into the vector pgex-kg and expressed in escherichia coli as a fusion protein coupled with the glutathione s-transferase of schistosoma japonicum. the fusion protein was purified on a glutathione-agarose affinity column and used to immunize rabbits for the production of polyclonal anti-5-hydroxytryptamine1a receptor antibodies. enzyme-linked immunosorbent ... | 1994 | 7870302 |
| overproduction, purification and structural characterization of the functional n-terminal dna-binding domain of the fru repressor from escherichia coli k-12. | a dna fragment encoding the dna-binding domain (amino acids 1-60) of the escherichia coli fru transcriptional regulator was cloned into the pgex-kt vector and expressed in frame with the fused gene encoding glutathione s-transferase. the fusion protein was purified to homogeneity by affinity chromatography on immobilized glutathione, and then cleaved with thrombin. after separation by a cation-exchange chromatography step, the dna-binding domain exhibited proper folding, as shown by proton nmr a ... | 1995 | 7883193 |
| detection of host dna sequences including the h-2 locus of the major histocompatibility complex in schistosomes. | the mouse type 2 alu (b2) sequence was detected in both dnas of schistosoma mansoni and s.japonicum except for the cercarial stage by the polymerase chain reaction (pcr). using several kinds of mouse stms (sequence tagged microsatellite site) primer sets, pcr products related to the host were found in the dnas of s. mansoni as well as of s.japonicum. products could be detected only in the dna of s. japonicum using certain stms primer sets. the fact that no products could be amplified from the dn ... | 1995 | 7885735 |
| updated codon usage in schistosoma. | | 1995 | 7895849 |
| cdna sequences of schistosoma japonicum coding for two cathepsin b-like proteins and sj32. | sequences of three cdnas of schistosoma japonicum are presented. two of them code for the antigens sj32 (hemoglobinase) and cathepsin b. the third clone represents a mutant derivative of the cathepsin b gene. the dna and amino acid sequences are compared to their homologues from s. mansoni. the mutant cathepsin b, in which the cys29 in the active center is replaced by ser, is discussed with respect to a possible function. | 1994 | 7899786 |
| international symposium on schistosomiasis. | | 1993 | 7900975 |
| single-step purification of recombinant melanoma growth-stimulating activity by anion-exchange high-performance liquid chromatography. | the cytokine melanoma growth-stimulating activity (mgsa) is a growth factor for melanoma cells and a chemotaxin for neutrophils. known purification procedures of mgsa from human sources or expression systems give a low yield and require multiple chromatography steps. here, a fast and high-yield method for the purification of recombinant mgsa is described. approximately 500 micrograms mgsa were recovered from the bacterial lysate of a 10 liter culture within a day. for this purpose, total mrna of ... | 1994 | 7921655 |
| encapsulated egg deposition pattern of schistosoma japonicum in the peritoneal cavity of mice. | thirty-four albino mice were each exposed to 20 cercariae of schistosoma japonicum by the intraperitoneal or percutaneous route and killed after 60-127 days. the peritoneal cavity of each mouse was flushed and worms in the portal vein system were perfused with citrated saline. after sedimentation, washings were examined microscopically. mature worms were only found in the portal vein system. the worm recovery rate was much higher by percutaneous infection (61.2%) than by intraperitoneal (37.1%). ... | 1994 | 7930453 |
| detection of schistosoma japonicum antigen (sj31/32) in sera of chinese patients using a sandwich elisa based on monoclonal antibody. | a sandwich-elisa was developed using igg from rabbits infected with schistosoma japonicum as capture antibody and a monoclonal antibody as detecting reagent for circulating sj 31/32 in patient sera. the assay detected circulating antigen in 36 of 53 sera from chinese schistosomiasis japonica patients, but not in sera from healthy persons or patients with paragonimiasis, trichinosis or cysticercosis. this is the first demonstration that sj 31/32 proteins may be used for circulating antigen-based ... | 1994 | 7939160 |
| vectors for cu(2+)-inducible production of glutathione s-transferase-fusion proteins for single-step purification from yeast. | we describe six new yeast episomal vectors which encode glutathione s-transferase (gst) affinity tags. these allow for the production of gst-fusion proteins in saccharomyces cerevisiae under the control of the cup1 promoter. affinity chromatography with glutathione-sepharose permits convenient purification of the fusion protein from a yeast lysate. the presence of a protease cleavage site facilitates subsequent removal of the gst tag. the expression and single-step purification of both gst and a ... | 1994 | 7941730 |
| genetic variation in geographically isolated populations and subspecies of oncomelania hupensis determined by a pcr-based rflp method. | previously, the species complex oncomelania hupensis, individuals of which act as intermediate hosts for the human bloodfluke schistosoma japonicum, has been characterised by morphological and isoenzyme criteria. we have examined genetic variation between and within three subspecies of oncomelania hupensis, i.e. o. h. hupensis (china), o. h. quadrasi (philippines) and o. h. nosophora (japan), by direct means using a pcr-based rflp method. the subspecies of o. hupensis were readily distinguished ... | 1994 | 7942357 |
| demonstration of the target molecule of a protective ige antibody in secretory glands of schistosoma japonicum larvae. | we have demonstrated that a mouse monoclonal ige antibody, sj18 epsilon.1, recognizes a 97 kda surface molecule (sj97) of schistosoma japonicum larvae and that the antibody induces partial but significant protection against the skin to lung-stage of s. japonicum infection. the antibody stimulates eosinophil- and macrophage-mediated killing of schistosomula in vitro. in the present study, we isolated the putative full-length cdna of sj97 by screening a lambda gt11 cdna library from s. japonicum a ... | 1994 | 7947464 |
| [preliminary observation on the detection of schistosome circulating antigen and the immunocomplex by dot-elisa]. | serum samples were collected from normal rabbits, normal persons, schistosoma japonicum-infected rabbits and human cases respectively, and were treated by tca precipitation and peg precipitation, and subsequently detected by dot-elisa. the results showed that both the supernatants of tca-treated antisera from infected human cases and rabbits and the precipitants of peg-treated antisera of the same origins showed positive reactions, while no positive reactions were found for sera from normal rabb ... | 1994 | 7955165 |
| purification and characterization of recombinant human apolipoprotein a-ii expressed in escherichia coli. | we have expressed recombinant human apolipoprotein a-ii (apoa-ii) in escherichia coli, as a fusion protein with schistosoma japonicum glutathione-s-transferase (gst). the gst-aii fusion protein was recovered by affinity chromatography using glutathione as a ligand. after thrombin cleavage and removal of the gst carrier, recombinant apoa-ii was obtained in a highly purified form and was exclusively composed of dimeric apoa-ii. kinetics of association to dimyristoylglycerophosphocholine (myr2gropc ... | 1994 | 7957205 |
| gene cloning, overproduction and purification of a functionally active cytoplasmic fatty acid-binding protein (sj-fabpc) from the human blood fluke schistosoma japonicum. | we report the gene cloning, molecular characterisation and purification of a 14.7-kda functionally active recombinant (re) cytoplasmic fatty acid-binding protein (sj-fabpc) from the chinese strain of the human bloodfluke schistosoma japonicum (sj). as schistosomes are unable to synthesise long chain fatty acids and sterols de novo and must, therefore, take up these lipids from the host, sj-fabpc is an attractive vaccine and/or drug target. clone 39 (c39), which contains the entire sj-fabpc gene, ... | 1994 | 7958962 |
| intestinal schistosomiasis japonica: ct-pathologic correlation. | to study the pathologic basis of intestinal wall calcification caused by schistosoma japonicum visualized with computed tomography (ct). | 1994 | 7972775 |
| evidence for and implications of anti-embryonation immunity in schistosomiasis. | | 1994 | 8001666 |
| early treatment of schistosomal infection with praziquantel in mice. | the first single dose of praziquantel (pra) was given ig to mice on the day of infection with schistosoma japonicum cercariae, or 7 d, 14 d, and 21 d after infection. afterwards, the same dose of pra was given once at 1-3 wk intervals for 2-3 times. the prophylactic effect was estimated by the reduction of average number of total and female worms, the number of mice without female worm, and the gross change of the liver. when mice were treated with pra 300-500 mg.kg-1 initially on d 21 after inf ... | 1993 | 8010053 |
| cerebral schistosomiasis caused by schistosoma haematobium: case report. | the cns is an unusual site of ectopic infection in schistosomiasis. cerebral lesions are caused primarily by schistosoma japonicum, and spinal cord lesions are due primarily to schistosoma mansoni and schistosoma haematobium. s. haematobium is an unusual cause of cerebral mass lesions although schistosomal eggs can be frequently found in the brains of individuals in countries where s. haematobium is endemic. we describe a patient with a space-occupying cerebral lesion and schistosomal granulomas ... | 1994 | 8011815 |
| parthenogenesis in the genus schistosoma: electrophoretic evidence for this reproduction system in s. japonicum and s. mansoni. | mixed infections with schistosoma japonicum and s. mansoni were carried out in mice. s. japonicum females paired with s. mansoni males developed normally and produced numerous viable eggs; very little sperm was found in the female genital tract. the eggs yielded many miracidia infective to oncomelania hupensis, the host of s. japonicum. cercariae arising from miracidia developed into male worms with an electrophoretic pattern of malate dehydrogenase (mdh) corresponding only to the maternal speci ... | 1994 | 8036230 |
| the serological differentiation of acute and chronic schistosoma japonicum infection by elisa using keyhole limpet haemocyanin as antigen. | antibodies (immunoglobulin (ig) g and igm) that recognize keyhole limpet haemocyanin (klh), which shares a well defined carbohydrate epitope with the surface of larval schistosomes, were determined by enzyme-linked immunosorbent assay in patients with acute or chronic schistosomiasis japonica. marked differences in igg and igm responses were evident between acute and chronically infected patients at a serum dilution of up to 1:2000. the acute sera had mean (+/- standard deviation) optical densit ... | 1994 | 8036691 |
| characterization of isoelectric point (pi) fractions of soluble egg antigen of schistosoma japonicum. | the soluble egg antigen of schistosoma japonicum was fractionated into 20 individual isoelectric point (pi) fractions with different pis ranging from 1.86-11.40 by isoelectric focusing (ief). the fractions were tested for humoral and cellular responses, as well as in vitro granuloma formations. the results indicated that the fractions in the acidic region (pi 3.78-5.54) may play an important role in granuloma elicitation in schistosomiasis japonica, and antibodies are also involved in the regula ... | 1994 | 8044897 |
| effect of early treatment of artemether against schistosomiasis in mice. | when artemether (art) was given ig to mice on the day of infection with schistosoma japonicum cercariae at a single dose of 300 mg.kg-1 and followed by the repeated dosing at 2-3 wk intervals, the total and female worm reduction rates as compared with the control were evident. in mice treated ig with art on d7 at the same dosage and repeated once every wk for 4 times, the female worm reduction rates were about or over 90%, and part of the animals was free from female worm. nevertheless, the live ... | 1994 | 8044912 |
| [preparation of membrane proteins of trichinella spiralis larva and application of them in detection with elisa]. | membrane proteins of muscle larva of trichinella spiralis (t.s) were isolated by using the non-ionic detergent triton x-114. at least fourteen components in the membrane proteins were separated with sds-page and then silver nitrate stain. the bands of them were located between 99-18kd mw level. the mw of chief bands were 94, 90, 47, 32 and 28kd. the sensitivity and specificity of these proteins were determined with elisa. they didn't cross react with sera of rabbits infected with toxocara canis ... | 1994 | 8070772 |
| nuclear magnetic resonance (nmr) analysis on the serum lipids of rabbits infected with schistosoma japonicum: oxidative modifications of diene system in fatty chains. | proton nuclear magnetic resonance (1h nmr) spectroscopy was used to characterize the oxidized products in the serum lipoprotein lipids (lpl) of rabbits infected with schistosoma japonicum. the loss of bis-allylic and allylic methylenes proton peaks (2.7 and 2.0 p.p.m.) of unsaturated systems of the lpl and the appearance of the signals of the conjugated diene systems of hydroperoxide (5.43-6.45 p.p.m.) and epidioxide (2.45 p.p.m.) of the fatty chains of the serum demonstrated the lipids degradat ... | 1994 | 8070961 |
| induction of hepatic pathology in scid-hu mice engrafted with peripheral blood lymphocytes of patients with schistosomiasis japonica. | scid mice were engrafted with peripheral blood lymphocytes (pbl) derived from persons currently or previously infected with schistosoma japonicum. after immunization with soluble worm antigenic preparation, the scid-hu mice were analyzed for a human immune response. elisa revealed a low titer of human antibody recognizing soluble egg antigens in 2 of 10 mice. one mouse had detectable levels of interleukin (il)-2 and gamma-interferon, th1 phenotype cytokines. all mice had elevated levels of il-4, ... | 1994 | 8077739 |
| [purification of 31/32 kda proteins of adult schistosoma japonicum and studies on their protective immunity]. | schistosoma japonicum adult worm 31/32 kda proteins (sj 31/32) were separated on polyacrylamide slab gels and purified by electrophoretic elution. these purified proteins were used to immunize mice in order to observe their protective immunity against challenge. the results of sds-page,eitb and elisa indicated that the 31/32 kda proteins separated and purified by means of these methods were pure and active. it was assumed that sj 31/32 proteins could reduce worm burden and inhibit the fecundity ... | 1993 | 8082259 |
| schistosoma japonicum-infected mice show reduced hepatic fibrosis and eosinophilia and selective inhibition of interleukin-5 secretion by cd4+ cells after treatment with anti-interleukin-2 antibodies. | schistosoma japonicum-infected mice were injected with antibodies to interleukin-2 (il-2) and/or il-2 receptor to clarify the role of il-2 on the granulomatous reaction around schistosome eggs in the liver. granulomas were of normal or slightly increased size in animals subjected to il-2 blockade, but hepatic fibrosis was markedly decreased in treated animals 10 weeks after infection. anti-il-2 treatment significantly decreased the in vitro secretion of il-5 by antigen-stimulated spleen cells, a ... | 1993 | 8095922 |
| [studies on the strain differences of schistosoma japonicum in the mainland of china. xii. dna hybridization of five isolates]. | the nonradioactive labelled probe psm 889 was hybridized to southern blots of genomic dna of five isolates of schistosoma japonicum from anhui, hubei, guangxi, sichuan and yunnan in the mainland of china. the major bands of hybridization of dna digested by restriction endonucleases ecori and bamhi are same among the five isolates, but the minor bands of hybridization of dna digested by ecori show some differences between 4.4-9.6 kb and between 2.3-4.4 kb among the five isolates. the result shows ... | 1993 | 8104737 |
| cloning, expression, and spectroscopic studies of the jun leucine zipper domain. | association of the human c-jun and c-fos proteins depends upon interactions involving their leucine zipper domains. we are interested in elucidating the tertiary structure of the jun and fos leucine zipper domains with a view to understanding the precise intermolecular interactions which govern the affinity and specificity of interaction in these proteins, which have the unusual capacity to form either homodimeric or heterodimeric zipper pairs. with this goal in mind, we have developed a bacteri ... | 1994 | 8112339 |
| recognition of different schistosoma mansoni antigens by specific human t cell clones. | human t lymphocyte clones (tlc) sensitized to schistosoma mansoni soluble egg antigens (sea) were developed from chronic schistosomiasis mansoni patients in order to investigate the regulatory mechanisms involved with in vitro granulomatous hypersensitivity to this parasite. all clones studied displayed cd4+ phenotype and required antigen-presenting cells in antigen-driven proliferation and granuloma formation. each t lymphocyte clone has been shown to proliferate and generate in vitro granuloma ... | 1994 | 8118891 |
| a t7 expression vector for producing n- and c-terminal fusion proteins with glutathione s-transferase. | the pgex system for protein production in e. coli is widely used in molecular biology. a bacterial expression vector, petgexct, which incorporates features of the pgex and pet expression systems was designed. petgexct allows the production of n- and c-terminal fusions to glutathione s-transferase (gst) under the tight control of the t7 promoter. use of this vector can circumvent problems associated with unstable or inactive fusions to the n terminus of gst. indeed, it is demonstrated that fusion ... | 1994 | 8125285 |
| expression and purification of the mouse mammary tumor virus gag-pro transframe protein p30 and characterization of its dutpase activity. | the mouse mammary tumor virus gag-pro transframe protein (p30) contains the nucleocapsid protein domain derived from the 3' end of gag, fused to 154 residues encoded by the 5' region of the pro open reading frame. the dna coding for p30 was cloned into the plasmid palter-1, and an additional nucleotide was inserted by site-directed mutagenesis to allow the read-through from the gag into the pro open reading frame. the obtained insert was then cloned into pgex-2t, a plasmid containing the glutath ... | 1994 | 8139016 |
| nuclear and mitochondrial genetic markers highly conserved between chinese and philippine schistosoma japonicum. | geographical isolates of s. japonicum, and particularly isolates from china and the philippines, were examined at the molecular level for genetic divergence. sequences from both nuclear and mitochondrial genomes were selected as markers of evolutionary divergence and s. mekongi and s. mansoni were included in the study for comparison purposes. restriction fragment length polymorphism (rflp) and pcr-rflp analysis of the rdna repeat unit and sequence analysis of the second internal transcribed spa ... | 1993 | 8147278 |
| kinetics of egg production and egg excretion by schistosoma mansoni and s. japonicum in mice infected with a single pair of worms. | individual male and female schistosomes approximately three weeks of age were implanted into the portal venous system of c57bl/6 mice to produce infections with a single pair of schistosoma mansoni or s. japonicum. mice were killed between seven and 54 weeks after infection. worm fecundity was measured by counting eggs accumulating in the tissues and eggs passed in the feces. schistosoma mansoni worm pairs laid approximately 350 eggs per day with no change in the apparent rate of egg laying betw ... | 1994 | 8147487 |
| reversal of advanced liver fibrosis in rabbits with schistosomiasis japonica. | advanced liver fibrosis is generally considered to be irreversible. we studied the reversibility of marked liver fibrosis in rabbits infected with schistosoma japonicum. we determined liver collagen content, collagen biosynthesis, and collagenase activity using serial biopsy specimens obtained 20, 40, and 60 weeks after infection. reversibility of this process was investigated in rabbits cured of infection at 21 weeks; control rabbits not cured of infection were also studied. at 20 weeks, liver ... | 1994 | 8166357 |
| [a study on the monoclonal anti-idiotypic antibody np30 for the diagnosis of schistosomiasis japonica]. | in this study the ability of the monoclonal anti-idiotypic antibody np30 was tested as a substitute of diagnostic antigen in detecting antibody of schistosoma japonicum from human sera by use of elisa. the results showed that the seropositive rate was 98% with np30 in the group of acute infection, which was comparable to 94% with gut associated antigens (gaa) and 98% with the soluble egg antigens (sea); 87% with np30 in the group of chronic infection which was comparable to 86% with gaa but lowe ... | 1993 | 8168242 |
| [role of macrophage in presenting antigens of schistosoma japonicum]. | indirect elisa was employed to monitor the serum anti-uea (urea soluble egg antigen of schistosoma japonicum) antibody level of mice immunized by: a. uea pulsed macrophage (mphi+); b. cultural supernatant of mphi+; c. paraformaldehyde fixed m phi (p-mphi) pulsed with uea; d. ammonium chloride treated m phi (nh4cl-mphi) pulsed with uea; e. p-mphi pulsed with trypsin digested uea (t-uea); f. nh4cl-mphi pulsed with t-uea. the normal mphi, its supernatant and the culture media rpmi 1640 acted as the ... | 1993 | 8174221 |
| [studies on the strain differences of schistosoma japonicum in the mainland of china. xiii. conclusion]. | up to the present, schistosoma japonicum endemic in the mainland of china has used to be considered being of only one strain, chinese mainland strain. it is well known that the endemic areas of schistosomiasis japonica in the chinese mainland are mainly confined to regions south of yangtze river, but they are discontinuous, showing conspicuous geographic and topographic isolation. so far, little is known about the characteristics of schistosomes isolated from various topographic areas. we have t ... | 1993 | 8174222 |
| sds-page protein pattern and its antigenicity analysis of different isolates of schistosoma japonicum in china. | homogenates prepared from s. japonicum adult worms of different isolates from anhui, hubei, guangxi, yunnan and sichuan provinces were analyzed by sds-page and enzyme linked immunoelectrotransfer blot (eitb) tested with rabbit anti-snails antibody. the results of sds-page indicated that with silver staining both male and female worms of guangxi isolate showed some definite differences in their protein profile, namely, absence of one band between 50-75 kda in male worms and marked reduction in qu ... | 1994 | 8187569 |
| purification of a recombinant schistosoma japonicum antigen homologous to the 22-kda membrane-associated antigen of s. mansoni, a putative vaccine candidate against schistosomiasis. | we describe the cdna cloning, overproduction and purification of a 22.6-kda antigen from the human blood fluke schistosoma japonicum. a 777-bp cdna (c32) was isolated from a s. japonicum lambda zapii cdna expression library immuno-screened with hyperimmune rabbit serum (hrs) raised against soluble adult s. japonicum proteins. the open reading frame of c32 encodes a protein of 191 amino acids (aa) which exhibits 71% identity to a 22.6-kda membrane-associated antigen of s. mansoni, a putative vacc ... | 1994 | 8194761 |
| cloning and expression of ole e i, the major allergen from olive tree pollen. polymorphism analysis and tissue specificity. | ole e i, the major allergen from the olive tree (olea europaea), is one of the main causes of allergy in mediterranean countries and some areas of north america. the cloning and sequencing of several cdnas coding for the olive allergen have been achieved. cdna has been synthesized from total pollen rna and amplified by using the polymerase chain reaction. the nucleotide sequence data demonstrate the existence of microheterogeneities in at least 37 positions out of the 145 amino acids of ole e i, ... | 1994 | 8195158 |
| cloning and functional expression of a schistosoma japonicum cdna homologous to the enolase gene family. | a cdna encoding the complete open reading frame of schistosoma japonicum enolase has been cloned. the 1494bp cdna (c30) was isolated from a s. japonicum cdna expression library immunoscreened with hyperimmune rabbit sera raised against soluble adult s. japonicum proteins. the orf encodes a protein of 434 amino acids exhibiting 72% identity to human, murine, and rat enolases, and 62% identity to saccharomyces cerevisiae enolase. the inferred molecular mass of the protein is 47,251 daltons, simila ... | 1993 | 8216251 |
| biology of schistosoma japonicum. from cercaria penetrating into host skin to producing egg. | the biology of the chinese mainland strain of schistosoma japonicum in mammalian phase of the life cycle has been studied in the past three decades. the mode of cercarial penetration is found to involve enzyme secretion and movement. the entry of schistosomulum into the blood vessel in the hypodermal connective tissue is evidenced after skin penetration. the growth and development of this fluke in mice and rabbits are investigated by physiological, histochemical and ultrastructural methods. the ... | 1993 | 8222905 |
| diagnostic evaluation of dot-binding assays for circulating cathodic antigen (cca) and anti-cca determinations in schistosomiasis japonica using defined biotinylated conjugates. | using an affinity purified circulating cathodic antigen (cca) preparation and an anti-cca monoclonal igm antibody, we modified dot-binding assays for anti-cca and cca detections with biotinylated conjugates. a study on 3 groups of 138 schistosomiasis japonica patients and 105 healthy individuals demonstrated a high predictive rate of over 95% in both assays, highly comparable to those of circumoral precipitin test (copt). a blind test in 342 samples of various groups revealed higher detection ra ... | 1993 | 8222906 |
| immunodiagnosis of schistosoma japonicum infection in china. | diagnosis is an essential prerequisite for the control programs of schistosomiasis. chinese scientists have been making efforts for decades towards the development of immunological approach in an attempt to offer alternatives of or supplements to parasitological diagnosis. the authors outline briefly the main advances in the research of immunodiagnosis for schistosomiasis japonica in china in two catalogues: antibody detection and antigen detection. a number of immunodiagnostic assays based on a ... | 1993 | 8222913 |
| schistosoma mansoni: immunoreactivity of human sera with the surface antigen sm23. | sm23, a surface antigen of schistosoma mansoni, elicits an immune response in patients infected with the parasite, although to a lesser degree than sj23, the homologue of sm23 in schistosoma japonicum. to characterize the host immune response to sm23, we have expressed full-length sm23 using the baculovirus expression system and have also expressed the n-terminal 133 amino acids and the 85 c-terminal amino acids by means of the prokaryotic vector pgex. a total of 70 sera from patients from sudan ... | 1993 | 8224084 |
| infectivity of the newly-isolated han-pao strain of schistosoma japonicum from taiwan to some oncomelanid snails. | | 1993 | 8250634 |
| biochemical properties of cloned glutathione s-transferases from schistosoma mansoni and schistosoma japonicum. | cdna clones encoding a 28-kda subunit glutathione s-transferase (gst) from schistosoma mansoni (sm28gst) and a 26-kda subunit gst from schistosoma japonicum (sj26gst) have been expressed in bacterial systems. the recombinant proteins were purified to homogeneity by batch-wash glutathione-agarose affinity chromatography and their biochemical properties investigated. gel filtration chromatography indicated that both recombinant gsts are homodimeric proteins. resolution of sm28gst and sj26gst by ch ... | 1993 | 8264729 |
| hepatosplenic lesions induced by experimental schistosoma malayensis in rabbits. | two groups of three rabbits each were infected with 250 cercariae of the baling and koyan strain of schistosoma malayensis. changes induced by both strains included periportal hepatocellular necrosis and fibrosis. vascular changes such as portal phlebitis and thrombophlebitis and varying degrees of pericholangitis were also present. amyloid deposition was noted. a comparative study of the changes induced in rabbits by s. malayensis, s. mekongi and s. japonicum showed that the hepatic lesions ind ... | 1993 | 8266238 |
| dot immunoassay with biotinylated antigen for determination of antibodies against the circulating cathodic antigen (cca) in schistosomiasis japonica. | based on the fact that schistosomiasis patients in both the acute and chronic phase of the infection show a strong humoral immune response against the gut-associated circulating cathodic antigen, a simple and sensitive dot immunobinding assay for schistosomiasis japonica was developed. circulating cathodic antigen that had been purified by immunoadsorption using monoclonal antibody was biotinylated with biotin aminocaproylhydrazide via the carbohydrate moiety of the antigen. serum samples dotted ... | 1993 | 8279643 |
| one-step purification of plant ferredoxin-nadp+ oxidoreductase expressed in escherichia coli as fusion with glutathione s-transferase. | complementary dna sequences encoding the mature form of pea ferredoxin-nadp+ reductase were cloned in-frame at the 3' end of the schistosoma japonicum glutathione s-transferase gene in the expression vector pgex-3x (smith and johnson, gene 67, 31-40, 1988). a spacer sequence linking the two genes was modified to provide a proteolytic site just before the first amino acid residue of mature pea reductase. when introduced into competent escherichia coli cells and induced, the resulting plasmid (pgf ... | 1993 | 8286951 |
| host-related dna sequences are localized in the body of schistosome adults. | the localization of host (mouse)-related dna sequences in the adults and cercariae of schistosoma japonicum and s. mansoni was examined by in situ hybridization using 32p-labelled probes. the hybridization signals to the sequences of the mouse type c and type a retroviruses were clearly observed in the subtegumental layer and inner tissues of s. japonicum adults. in contrast, it was hard to find any signals to these sequences in s. mansoni adults. distinct signals to the env-specific region of t ... | 1993 | 8295791 |
| comparative analysis of hepatic, pulmonary, and intestinal granuloma formation around freshly laid schistosoma japonicum eggs in mice. | hepatic, pulmonary, and intestinal granuloma formation was comparatively studied in mice implanted with freshly laid schistosoma japonicum eggs. the liver and the lung showed similar kinetics of tissue reactivity, with the magnitude in the lung being of a significantly lower degree. when the footpad-swelling test was performed, the delayed-type hypersensitivity (dth) response during hepatic granuloma formation was found to be significantly increased from the 28th day after egg implantation onwar ... | 1993 | 8327455 |
| immune attrition of adult schistosomes. | mouse infection models are described that demonstrate reduction in the rate of egg production in schistosoma haematobium worms 6-10 weeks after the onset of oviposition and loss of schistosoma bovis worms around 10 weeks after infection. neither phenomenon has been shown in schistosoma mansoni- or schistosoma japonicum-infected mice. the immunological basis for these anti-adult responses was inferred by comparison with infections in t-cell deprived mice and by transference of the ability to redu ... | 1993 | 8332382 |
| unilateral optic atrophy presumed secondary to schistosomiasis of the optic nerve. | schistosomiasis, a parasitic disease, is endemic in many parts of the world. schistosomal eggs may be found in almost any organ or tissue in the body, including the eye. the presence of schistosomal eggs in the eye can produce granuloma formation and inflammatory sequelae. | 1993 | 8335885 |
| immunization of pigs against infection with schistosoma japonicum using ultraviolet-attenuated cercariae. | since pigs are important in the zoonotic transmission of schistosomiasis japonica in china, a veterinary vaccine might contribute to the control of the disease in humans. pigs were immunized with three doses each of 10,000 cercariae of schistosoma japonicum attenuated with ultraviolet light (400 microwatt.min/cm2). the experiment was performed with portable irradiation equipment in a rural area of the hubei province (p.r. china). a challenge infection of 1,000 untreated cercariae was given 2.5 o ... | 1993 | 8341581 |
| lipase from pseudomonas aeruginosa. production in escherichia coli and activation in vitro with a protein from the downstream gene. | the lipase gene from pseudomonas aeruginosa te3285 is followed by another gene, lipb. the lipase gene was expressed in escherichia coli bl21(de3)plyss using the t7 rna polymerase expression system. the mature lipase was accumulated as inclusion bodies at 42% of the total cell proteins. the inclusion bodies were solubilized with 8 m urea, but lipase activity was not detected in the solubilized preparation containing 85% lipase protein even after removing urea by dialysis. the lipb gene, positione ... | 1993 | 8344292 |
| schistosoma japonicum soluble egg antigens activate naive b cells to produce antibodies: definition of parasite mechanisms of immune deviation. | this study analysed the effect of schistosoma japonicum egg antigens (sea) on the activation of lymphocytes from naive mice. t cells were found to be unaffected by sea. b cells, however, were activated by sea without participation of adherent cells such as macrophages. b-cell activating factor(s) in sea were distributed into a fraction of m(r) 120,000 and a fraction of m(r) 650,000 by gel filtration. however, a fraction of m(r) 120,000 demonstrated the presence of a limited number of components ... | 1993 | 8344698 |
| a new function of s-adenosylmethionine: the ribosyl moiety of adomet is the precursor of the cyclopentenediol moiety of the trna wobble base queuine. | queuosine (q) [7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deaz agu anosine] usually occurs in the first position of the anticodon of trnas specifying the amino acids asparagine, aspartate, histidine, and tyrosine. the hypermodified nucleoside is found in eubacteria and eucaryotes. q is synthesized de novo exclusively in eubacteria; for eucaryotes the compound is a nutrient factor. in escherichia coli the q precursor (oq), carrying a 2,3-epoxy-4,5-dihydroxycyclopentane ring, is for ... | 1993 | 8347586 |
| regression of hepatic lesions after treatment of schistosoma mansoni or schistosoma japonicum infection in mice: a comparative study. | experimental infections with schistosoma mansoni and s. japonicum differ in several aspects and post-treatment resorption of fibrosis might be one of them. to investigate this point, mice infected with each of these schistosome species were treated with praziquantel and the evolution of hepatic lesions was sequentially followed for five months. parasitologic data showing destruction of worms and eggs and biochemical findings of progressively decreased collagen concentration after cure indicated ... | 1993 | 8352381 |
| imported opisthorchis viverrini and parasite infections from thai labourers in taiwan. | in order to determine the prevalence of intestinal parasites among labourers from thailand in taiwan, the stool samples from 1364 thai labourers were examined by the formalin-ether concentration method and 18.0% were found to be infected with one or more parasites. the infection rates of hookworm, opisthorchis viverrini, strongyloides stercoralis, enterobius vermicularis, ascaris lumbricoides, hymenolepis nana, capillaria philippinensis, schistosoma japonicum, echinostoma sp., entamoeba coli, gi ... | 1993 | 8354853 |
| studies on chemotherapy of parasitic helminths: efficacy of artemether on japanese strain of schistosoma japonicum in mice. | effects of artemether were examined on schistosoma japonicum in mice. when the drug was given at a daily dosage of 200 mg/kg for 4 successive days from 46 days post-infection, a significant reduction in worm recovery was observed. a significant reduction in size of worms from the medicated mice was also seen compared with that from non-medicated controls. | 1993 | 8362307 |
| the temperature--dependent expression of gst of schistosoma japonicum (philippine strain). | obtained from psj5, the cdna gene encoding gst antigen of schistosoma japonicum (philippine strain) was ligated with efficient temperature-dependent pbv220 vector which was constructed in capm, and then introduced into host bacterium-dh5 alpha (e. coli) by transformation. transformants were selected by ampicillin and recombinant clones were identified by restriction mapping. the result showed that recombinant clone 43 was the one carrying recombinant plasmid pbv 220 with the correct insertion of ... | 1993 | 8362308 |
| the antigenicity of gst antigen extracted from chinese strain of schistosoma japonicum. | the gst antigen, similar to sj26 (philippine strain), which plays an important role in inducing protective immunity against schistosoma japonicum, can be extracted and purified from adult worms of the chinese strain of s. japonicum. there are two bands at 26 kda and 28 kda of gst antigen called the 26-28 kda gst antigen as identified by sds-page, and these have gst activities. mice were immunized with the 26-28 kda antigen and the specific antibody response in serum was assayed by elisa, ifa and ... | 1993 | 8362309 |
| comparative study on antigenicity and immunogenicity of 26-28 kda antigen and recombinant sj26 (rsj26) of schistosoma japonicum. | this paper reports a comparison of the recombinant sj26 (rsj26) antigen derived from the philippine strain and the 26-28 kda antigen isolated and purified from the chinese strain of schistosoma japonicum with respect to their antigenicity and immunogenicity. the results showed that there were obvious cross reactions between rsj26 and 26-28 kda antigen when rsj26 antigen was tested against specific antibodies in sera of mice infected with the chinese strain of s. japonicum or the 26-28 kda antige ... | 1993 | 8362310 |
| the possibility of gst antigen from chinese strain of schistosoma japonicum for immunodiagnosis of schistosomiasis. | the gst antigen (called 26-28 kda antigen) extracted and purified from schistosoma japonicum adult worms was applied to the detection of specific antibodies in sera of infected mice and mice immunized with the above protein antigen by elisa technique. the 26-28 kda antigen was better than crude antigens (sea, swap) when used to detect specific antibodies in sera from immunized mice. as with crude antigens (sea and swap), the 26-28 kda antigen could be used to detect specific antibodies in infect ... | 1993 | 8362311 |
| the hoeppli phenomenon in schistosomiasis japonica: historical findings and additional investigation in bovines. | the present paper deals with a buried knowledge of the early findings of the hoeppli phenomenon in cattle infected with schistosoma japonicum together with a revised list of bovines and other mammalian species in which this phenomenon has been found. it was noted that the percentage of the mature-egg granulomas with positive hoeppli phenomena varied with the species of bovines, ie, higher positive percentage in the more susceptible cattle than in the less susceptible buffalos. the radiating fila ... | 1993 | 8362312 |
| moloney murine leukemia virus protease: bacterial expression and characterization of the purified enzyme. | the moloney murine leukemia virus (mo-mulv) protease has been cloned into the prokaryotic expression vector pgex-2t, expressed in fusion with the glutathione s-transferase from schistosoma japonicum, and purified to apparent homogeneity after thrombin cleavage of the chimeric protein. the purified protease showed maximum activity at ph 6.0 and was inhibited by several aspartyl protease inhibitors, found to be active toward the human immunodeficiency virus-1 (hiv-1) protease. peptides representin ... | 1993 | 8372434 |
| a study and analysis of the deaths due to advanced schistosoma japonicum infection in the dongting lake area of china. | this study collected records of 245 cases of death due to advanced schistosomiasis japonica in the eastern dongting lake area between 1985 and 1990. the mean survival of the patients was 5.16 years. the patients with ascitic type had a shorter expected life while the life span of splenomegalic type patient markedly improved after splenectomy. the hbsag positive rate was 43.64%; the rate of those complicated with carcinoma of liver was 19.18%. the latter group had a hbv infection rate as high as ... | 1993 | 8384665 |
| acquired resistance to schistosoma japonicum in ige-deficient sja/9 mice immunized with irradiated cercariae. | participation of ige in protective immunity against schistosoma japonicum was examined by comparing congenital ige-deficient sja/9 and ige-producing sjl/j mice. mice immunized with 100 irradiated cercariae 7 weeks previously were infected with 50 live cercariae. in sjl/j mice at 40 days after infection, a 3-to 4-fold increase of total ige levels and anti-s, japonicum egg ige antibody production were observed with no significant difference between immunized and nonimmunized mice. ige was not dete ... | 1993 | 8400898 |