| virulence of pathogenic and non-pathogenic zymodemes of entamoeba histolytica (indian strains) in guinea-pigs. | guinea-pigs were inoculated with entamoeba histolytica strains isolated from cases of amoebic liver abscess, amoebic dysentery and asymptomatic cyst passers and the strains were classified in zymodemes by isoenzyme electrophoresis. it was observed that certain non-pathogenic zymodemes were potentially pathogenic for the guinea-pigs. the relationship of zymodemes and virulence in laboratory animals is discussed. | 1989 | 2559510 |
| effects of panmede and various horse serum concentrations on the axenic cultivation of entamoeba histolytica strains in tps-1 medium. | we analyzed the influence of panmede and horse serum concentrations on the growth of five entamoeba histolytica strains (hk9, hm1, hm2, hm3, and hm38) axenically cultivated in tps-1 medium. panmede was evaluated by comparing the growth of strain hm1 in medium prepared with each of 15 panmede lots; the yields of e. histolytica trophozoites depended on the lot quality of panmede, and their maximal values ranged from 8 x 10(3) to 8.9 x 10(4) amoebae/ml. the growth-promoting effect of eight lots of ... | 1989 | 2560188 |
| interaction of rheumatoid factor and entamoeba histolytica. | the amoebae's cytotoxicity test and the amoebae's lysis test were used to show possible interactions between rheumatoid factor (rf) and entamoeba histolytica. amoebae's cytotoxic activity (aca) was inhibited by affinity chromatography purified antiamoebae rabbit igg (rigg). enhanced inhibition could be demonstrated with rigg plus rf. but the same marked inhibition of aca could be seen when replacing rf by heat inactivated normal human serum as a control. about 50% amoebae's lysis occurred when a ... | 1989 | 2560586 |
| effect of exogenous 5-hydroxytryptamine on pathogenicity of entamoeba histolytica in experimental animals. | in an effort to find out the mechanism(s) operative in enhancing the pathogenicity of e. histolytica in hosts under heat stress reported earlier, effect of 5-hydroxytryptamine (5-ht) on the virulence of the parasite was examined in just weaned charles foster strain of albino rats. pathogenicity of 10 strains of e. histolytica, from various forms of intestinal amoebiasis, grown in modified boeck and drbohlav's medium was assessed by caecal scoring. administration of 5-ht in infected animals signi ... | 1989 | 2561282 |
| an outbreak of trichuris trichiura in a mental handicap hospital. | an outbreak of trichuris trichiura in a ward for the severely mentally handicapped affected 22 of 29 patients. there was concurrent carriage of non-pathogenic entamoeba histolytica in 59% of these patients. heavy contamination of soil was found in the patients' recreation area. the management of the outbreak is discussed. | 1989 | 2567308 |
| adherence of entamoeba histolytica trophozoites to rat and human colonic mucosa. | we studied the adherence of [3h]thymidine-labeled axenic entamoeba histolytica (strain hm1-imss) to in vitro preparations of rat and human colonic mucosa. studies were performed with fixed or unfixed rat colonic mucosa, unfixed rat mucosa exposed to trypsin, unfixed rat submucosa, and fixed human colonic mucosa. twenty percent of the amebae adhered to fixed rat colonic mucosa; adherence was specifically inhibited by n-acetyl-d-galactosamine (galnac), galactose, and asialofetuin. the adherence of ... | 1985 | 2580787 |
| subcellular fractionation of amoebapore and plasma membrane components of entamoeba histolytica using self-generating percoll gradients. | separation and initial characterization of subcellular organelles from entamoeba histolytica have been achieved by the use of self-generating percoll gradients. adequate resolution was obtained within one hour of amoeba homogenization. the ion-channel forming activity, amoebapore, was found associated with highly dense, small diameter particles, that were resolved from the numerous digestive vacuoles, and the plasma membrane. following iodination of intact trophozoites with lactoperoxidase, the ... | 1985 | 2581133 |
| fluorometric analysis of transferable membrane pores. | when pore-forming factors insert into the hyperpolarized membranes of lipid vesicles, ion gradients are rapidly equilibrated, effecting complete depolarization. this process can be conveniently followed with a potentiometric cyanine dye. the generality of the method is demonstrated by applications to three diverse materials. the well-studied gramicidin channel is used to demonstrate that the method is sensitive down to concentrations of 10(-12)m. an extract from the shark repellent skin secretio ... | 1985 | 2581609 |
| characterization of a thiol proteinase in giardia lamblia. | sonicated preparations of giardia lamblia hydrolyze a variety of protein substrates including human immunoglobulin. activity is increased by thiol-activating agents and inhibited by thiol proteinase inhibitors. about 55% of activity remains in the soluble fraction after high-speed centrifugation, and pretreatment with a nonionic detergent results in increased soluble activity. this suggests that the enzyme is membrane bound or associated with subcellular particles. activity elutes as a major pea ... | 1989 | 2584755 |
| exotic diarrhoeal problems and poliomyelitis. | exotic gastrointestinal infections continue to increase as world travel expands. many are debilitating and some are life-threatening. a heightened awareness of their significance and symptomatology could help to prevent unnecessary suffering or death. | 1989 | 2594659 |
| prevalence and seasonal fluctuations of intestinal parasitic infections in the nablus area, west bank of jordan. | a total of 22,970 stool specimens collected from patients attending the central medical laboratory in the city of nablus in the period of 1981-1986 were examined for intestinal parasites. of these 7412 (32.3%) were positive. entamoeba histolytica (22.9%), giardia lamblia (7.3%), and ascaris lumbricoides (5.7%) were the most prevalent intestinal parasites found. other intestinal parasites present included hymenolepis nana, trichomonas hominis, trichuris trichiura, taenia saginata, enterobius verm ... | 1989 | 2596904 |
| comparison of four nitroimidazole compounds for treatment of symptomatic amoebiasis in kenya. | four antiamoebic drugs currently used in many kenyan hospitals and health centres were compared for their efficacy on symptomatic luminal amoebiasis in kiambu, kilifi, and machakos hospitals during this study. the drugs were; the brand metronidazole (flagyl, may & baker, kenya ltd.), the generic metronidazole (metrozol, cosmos ltd., nairobi, kenya), the brand tinidazole (fasigyn, pfizer laboratories ltd.) and the generic tinidazole (tynazole laboratory and allied equipments, kenya ltd). clinical ... | 1989 | 2606014 |
| [synthesis of 2-aryl benzothiazoles and research on its antiparasitic activity]. | several 2-aryl benzothiazoles (with phenyl, naphthyl, thienyl and some oxygenated heterocycles) were synthesized. the anti-parasitic properties of these compounds were evaluated, in vitro and in vivo, against one nematode nippostrongylus brasiliensis and, in vitro against two protozoaires entamoeba histolytica and trichomonas vaginalis. only two compounds exhibited, in vitro a low nematicidal activity. | 1989 | 2610470 |
| a model of hepatic amoebiasis in random bred mice. | this study describes a model of hepatic amoebiasis in random bred mice (mf2). mice were infected by introducing liver tissue from hamsters (mesocricetus auratus), containing about 5 x 10(4) trophozoites of entamoeba histolytica, between adjacent liver lobes. direct inoculation of xenic e. histolytica resulted in mortality within 24 h, whereas monoxenic and axenic strains failed to produce any lesions. serial mouse liver passage resulted in increased lesion score, number of metastatic abscesses, ... | 1989 | 2617583 |
| experimental production of new zymodemes of entamoeba histolytica supports the hypothesis of genetic exchange. | by mixing in culture zymodemes of entamoeba histolytica, clones showing a different pattern from the 2 parents have been obtained on 3 occasions. two new zymodemes (xi alpha- and xxi) have thus been produced, which belong to the third generation of a family of e. histolytica zymodemes apparently obtained by genetic exchange. a hypothesis for the molecular basis of those exchanges is discussed. we report also the observation of probable mutation. | 1989 | 2617647 |
| determination of antiamoebic igg, igm, iga and igg subclasses in sera from patients with amoebiasis by enzyme-linked immunosorbent assay. | the hk-9 strain of entamoeba histolytica was cultured axenically. a crude extract of the trophozoites was used as antigen in the enzyme-linked immunosorbent assay (elisa) for the determination of antiamoebic igg, igm, iga and igg subclasses in sera of patients with e. histolytica infection. sera from amoebiasis patients had significantly higher mean elisa values for all classes and subclasses of immunoglobulins examined compared with healthy controls. the elisa for igg, igm and iga was positive ... | 1989 | 2633348 |
| [prevalence of entamoeba histolytica and other intestinal protozoa among the inhabitants of poznan province over a period of 30 years]. | the aim of the study was to present the past and actual prevalence of entamoeba histolytica and other intestinal protozoa in poznań province. although in poland there are only few accurate data concerning the prevalence of intestinal protozoa, the results of some studies were found reliable. in a survey conducted in 1958 in an orphanage in poznań (kasprzak and karlewiczowa) the intestinal protozoa were found with high prevalence (table 1). after six years this prevalence decreased considerably ( ... | 1989 | 2633499 |
| [pathogenic agents in acute non-enterocolic diarrheal syndrome]. | along a one year period 112 infants admitted with non enterocolic acute diarrhea were studied for isolation of potentially ethiologic agents, namely enteropathogenic bacteria (salmonella, shigella, campylobacter, classic enteropathogenic, enteroinvasive and enterotoxigenic escherichia coli), rotavirus (viral rna electrophoresis) and enteroparasites (telemann and pafs). the most frequently identified pathogen was rotavirus (57.8%), followed by thermo labile toxin producing escherichia coli (19.7% ... | 1989 | 2634863 |
| descriptive epidemiology of persistent diarrhoea among young children in rural northern india. | in order to determine the descriptive epidemiology of persistent diarrhoea in rural northern india, a cohort of 963 children aged 0-71 months was followed prospectively for 12 months through weekly household visits. the incidence of persistent diarrhoea was 6.3 per 100 child-years among those aged 0-71 months, and was highest (31 per 100 child-years) among those aged 0-11 months. there were no significant sex-related differences in the incidence of the disease, and the overall seasonal distribut ... | 1989 | 2670297 |
| [production and characterization of monoclonal antibodies to echinococcus multilocularis]. | spleen cells from balb/c mice immunized against echinococcus multilocularis were fused with mouse myeloma cells (p3x6.5.3.) in the presence of polyethylene glycol (peg 1000), and the hybridomas were obtained. of the 85 hybridomas, 23 were found to produce antibodies. of the 23 cell lines, 13 hybridomas which produced a high titer of antibodies were cultured for cloning the cells by the limiting dilution method. then 5 monoclonal antibodies were obtained. the immunoglobulin class of the monoclona ... | 1989 | 2684834 |
| an outbreak of amebiasis in an institution for the mentally retarded in japan. | the results of an epidemiological survey in a 190-patient institution for mentally retarded were reported. twenty percent of the patients had either cysts or trophozoites of entamoeba histolytica in their stools, and 38% were positive serologically. the amebic outbreak revealed a sex-independent but age-dependent distribution; younger patients had more serious symptoms in cases invasive amebiasis. a high prevalence of amebic infection was found in the heavily retarded patients, and the positive ... | 1989 | 2698965 |
| ige level in parasitic diseases before and after specific therapy. | fifty patients (3-12 years old) with parasitic infestation vz: e. histolytica, oxyuris, h. nana, ascaris and mixed infection were compared to ten healthy age and sex matched normal school children. they were subjected to stool and urine analysis total and differential white count, serum protein and serum ige estimation before and after the specific treatment. the absolute eosinophilic count was high during infestation in all groups and significantly decreased after treatment (p less than 0.01). ... | 1989 | 2708864 |
| [invasive amebiasis as a public health problem]. | amebiasis, an infection of humans with the protozoan entamoeba histolytica, has a wide distribution in mexico. the lumenal, asymptomatic infection, as measured by the presence of cysts in stools has been recorded from 2.4% at ometepec, guerrero to over 55% at mixquic, d.f., but only a small percentage of those having intestinal infection will develop invasive amebiasis, the main clinical forms being dysentery and liver abscess. in mexico city, from 0.8 up to 14% of cases of acute diarrhoea in ch ... | 1989 | 2713060 |
| intestinal infections in patients with acquired immunodeficiency syndrome. a prospective study in 132 patients. | we studied prospectively 132 patients with acquired immunodeficiency syndrome to define the spectrum of enteric pathogens during this disease, with special reference to the correlation between the lesions, the infections, and the symptoms. forty-four percent of the patients harbored at least one enteric pathogen: the most frequently recovered were cryptosporidium (28), cytomegalovirus (16), entamoeba histolytica (13), giardia lamblia (9), and mycobacterium avium intracellulare (7). patients harb ... | 1989 | 2714152 |
| [blastocystis hominis, a parasitic cause of diarrhea]. | the frequency of detection of blastocystis hominis in the stools of patients with gastroenteritis is reported. over a twelve-month period b. hominis was identified in the stools of 69 (4.72%) out of 1460 patients. of these 69 positive samples, 45 (65%) contained b. hominis alone and in 24 (35%) it was present together with other parasites such as lamblia intestinalis, entamoeba histolytica, entamoeba coli, endolimax nana, iodamoeba buetschlii, ancylostoma duodenale, ascaris lumbricoides, trichur ... | 1989 | 2717900 |
| the vitamin b1 status among young egyptian males in relation to infection with parasites. | erythrocytic transketolase activity and thiamin pyrophosphate effect (tpp%) were used to define the nutritional thiamin status of an age stratified sample of 66 young males with parasitic infection. the results revealed that 6% of the control group were biochemically deficient in vitamin b1 compared with a percentage incidence of 42% among the parasitized subjects. intestinal infection with entamoeba histolytica, hymenolepsis nana or with more than one parasite had the most depleting effect on t ... | 1989 | 2722425 |
| entamoeba histolytica: diffuse liver inflammation in gerbils (meriones unguiculatus) with experimentally induced amebic liver abscess. | the histopathological changes that occur in the uninfected liver lobes of gerbils with a localized amebic liver abscess (left lobe) were studied from 5 to 60 days after inoculation. early parenchymal changes (10 days) in the right and median liver lobes showed a lymphocytic infiltrate and bile duct proliferation in the portal areas. maximum alterations occurred at 20 to 30 days after inoculation and were characterized by a dense portal infiltrate, hepatocyte degeneration, granulocytic infiltrate ... | 1989 | 2724182 |
| squamous cell carcinoma associated with amoebic cervicitis. report of a case. | a case of squamous cell carcinoma associated with amebiasis is presented. protozoa identified as entamoeba histolytica were found in a routine papanicolaou smear and in the cervical biopsy of a young woman following the initial diagnosis of cervical cancer and the institution of a radiation therapy regimen. no amoebae were found in repeated stool specimens. the late detection and diagnosis of amebiasis precluded any possible changes in the management of the case. initial follow-up for the carcin ... | 1989 | 2728784 |
| [severe forms of hemorrhagic rectocolitis. predisposing role of the amebic graft?]. | severe forms of ulcerative colitis are somewhat rare but have a severe prognosis. apart from the usual predisposing factors, the amebic graft must be especially emphasized, at least in those countries where the parasite is endemic. entamoeba histolytica was consistently identified in 19 cases of the severe form that we found out of a total of 126 cases of ulcerative colitis. usually the parasite was found in the shape of massive infestation and in most cases it proved to be resistant to the ameb ... | 1989 | 2764495 |
| pattern of intestinal parasitic infection in preschool children in riyadh, saudi arabia. | a total of 1,167 stool specimens collected from 0.6-6 years old patients attending king abdel aziz university hospital (kauh) in riyadh, were examined for intestinal parasites. of these 243 (20.8%) were positive. giardia lamblia (13.5) and enterobius vermicularis (4.2%), were the commonest parasites found. other parasites present include ascaris lumbricoides, entamoeba histolytica and hymenolepis nana. abdominal pain (38.6%) and diarrhoea (27.6%) were the most common causes of referral presented ... | 1989 | 2809181 |
| etiology of acute childhood diarrhoea in calcutta. | of the 152 cases of acute diarrhoea, 124 (81.5%) revealed potential pathogens. altogether 27 (21.2%) out of 127 strains of escherichia coli, klebsiella pneumoniae, enterobacter, proteus and acinetobacter produced enterotoxin. single pathogenic bacteria (40 cases 26.3%), parasite (6; 6%), rota virus (6; 6%), toxigenic bacteria (19; 12.5%) and mixed agents (37; 24.24.3%) were recorded in 108 cases (71.0%). another 14 (9.2%) cases exclusively revealed moderate to heavy growth of suspected enteric p ... | 1989 | 2815324 |
| investigation of human stool samples from the cape verde islands (district santa cruz/santiago). | based on the rate of occurrence of diseases associated with diarrhoea, stool samples were also investigated; parasitologically, using the mifc-enrichment method for intestinal parasites; serologically, using elisa for rotavirus and bacteriologically for shigella and salmonella on the cape verde islands. 90% of the samples came from children aged between 6 and 14 years. entamoeba histolytica, the causal agent of amoebic dysentery was demonstrated in 17.4%, lamblia intestinalis in 28.7% and rotavi ... | 1988 | 2844628 |
| entamoeba histolytica trophozoites in the lumen and mucus blanket of rat colons studied in vivo. | trophozoites of entamoeba histolytica hm-1 were cultivated axenically in tyi-s medium. the amoebae were then transferred into this medium lacking serum (tyi) and inoculated into in vivo colon loops of adult sprague-dawley rats. the trophozoites were rapidly absorbed by the mucus, and few were found free in the luminal fluid by 1 h. by 4 h, the amoebae began to reappear in the lumen, aggregated in sloughed mucus blanket fragments. the colon was examined histologically and by scanning electron mic ... | 1985 | 2856915 |
| seroepidemiological study of antibody responses to the zymodemes of entamoeba histolytica. | in 303 apparently healthy subjects and 54 patients with confirmed invasive amoebiasis amoebae were isolated where possible, and entamoeba histolytica was classified into its various zymodems. 20% of the healthy subjects were antibody-positive; 17 healthy subjects proved to be symptomless carriers of pathogenic zymodemes and, like patients with invasive disease, all these were seropositive. furthermore, of subjects infected with pathogenic zymodemes 94-100% were strongly seropositive, compared wi ... | 1985 | 2857995 |
| frequency distribution of entamoeba histolytica zymodemes in a rural south african population. | to determine the prevalence of entamoeba histolytica and its zymodeme distribution pattern, isoenzyme electrophoresis was performed on isolates of the parasite from 1381 individuals of the population of a semirural endemic area south of durban. about 10% of the population was found to harbour e histolytica. the zymodeme distribution was similar to that described in other surveys but two new zymodemes were observed, a non-pathogenic zymodeme xix and a pathogenic zymodeme xx. non-pathogenic zymode ... | 1985 | 2857996 |
| is that amoeba harmful or not? | | 1985 | 2858001 |
| [tropical mimics; study of the feces is a must]. | | 1985 | 2858059 |
| isolation, purification, and partial characterization of an enterotoxin from extracts of entamoeba histolytica trophozoites. | soluble cell-free extracts of pathogenic entamoeba histolytica, as well as serum-free minimal media in which trophozoites are incubated, contain substances that cause the rapid rounding up and detachment of tissue-cultured monolayers of mammalian cells (cytopathic activity) and induce fluid secretion in ligated intestinal loops of indomethacin-pretreated rats (enterotoxic activity). a semiquantitative assay for the determination of the cytopathic activity based on the rate of detachment of tissu ... | 1985 | 2858451 |
| entamoeba histolytica: early progressive pathology in the cecum of the gerbil (meriones unguiculatus). | the early formation of amebic cecal lesions in the gerbil (meriones unguiculatus) was studied sequentially from 1 to 10 days post-inoculation. the earliest response of the cecal mucosa towards trophozoites of entamoeba histolytica was local release of interglandular and goblet cell mucin. trophozoites adhered to the surface epithelium or were trapped in the luminal mucus in the absence of epithelial cell damage. focal mucosal erosion occurred later, and was preceded by crypt hyperplasia, edema a ... | 1985 | 2858986 |
| n-acetyl-d-galactosamine-inhibitable adherence lectin of entamoeba histolytica. i. partial purification and relation to amoebic virulence in vitro. | adherence of axenic entamoeba histolytica, strain hm1-imss, to chinese hamster ovary (cho) cells is mediated by an amoebic adhesin that is inhibited by n-acetyl-d-galactosamine (galnac). the in vitro virulence for cho cells and human neutrophils (pmns) of four strains of axenic amoebae was strain hm1-imss greater than h303-nih = 200-nih greater than nonvirulent laredo (p less than .001). the hm1 strain had the greatest sensitivity to galnac-mediated inhibition of adherence (p less than .001). ga ... | 1985 | 2859338 |
| n-acetyl-d-galactosamine-inhibitable adherence lectin of entamoeba histolytica. ii. mitogenic activity for human lymphocytes. | a soluble protein preparation from virulent axenic amoebae (strain hm1-imss) has been demonstrated to be mitogenic for normal human lymphocytes; mitogenesis was optimal at 100 micrograms/ml when incubation was maintained for five to seven days with monocytes (p less than .001). after gel filtration chromatography mitogenic activity of fractions was associated with n-acetyl-d-galactosamine-inhibitable lectin activity (agglutination of chinese hamster ovary cells; p less than .001). lymphocyte pro ... | 1985 | 2859339 |
| entamoeba histolytica and e. invadens: sulfhydryl-dependent proteolytic activity. | sodium dodecyl sulfate (sds) and 2-mercaptoethanol (2-me) activated proteolytic enzymes present in extracts of entamoeba histolytica and e. invadens; sds (0.5%) and 2-me (1.4 and 715 mm) doubled the enzymatic activity when assayed on a stained insoluble substrate. urea (4 m) did not reduce this activity, suggesting that amebic proteases are stable in the above denaturant conditions. specific reagents for sulfhydryl (-sh) groups completely inhibited proteolytic activity regardless of ph. inhibiti ... | 1985 | 2859367 |
| structural bases of the cytolytic mechanisms of entamoeba histolytica. | the cellular bases of the powerful cytolytic activity of the human protozoan parasite entamoeba histolytica were explored by studying the effect of the virulent strain hm1:imss on epithelial monolayers of mdck cells using a combination of time-lapse microcinematography and transmission and scanning electron microscopy. early alterations of the epithelial cell membranes were detected by measuring changes in the transepithelial electrical resistance of mdck monolayers mounted in ussing chambers. t ... | 1985 | 2859368 |
| amoebic isoenzymes. | | 1985 | 2859504 |
| urinary tract infection with entamoeba histolytica after ileocaecal bladder replacement. | | 1985 | 2859507 |
| isolation and characterization of entamoeba histolytica mutants resistant to emetine. | isolation of emetine-resistant mutants of entamoeba histolytica is described. spontaneous rate of mutation, obtained from a luria-delbrück fluctuation test was 2.5 x 10(-7). the mutagenesis frequency obtained from the number of colonies grown in the presence of emetine, divided by the number of viable trophozoites inoculated in semisolid agar was less than or equal to 10(-7) and it was increased from 10 to 100 fold with ethyl methanesulfonate. two types of clones were isolated: one was cross-res ... | 1985 | 2859522 |
| tolerance of axenically cultured entamoeba histolytica and giardia lamblia to a variety of antimicrobial agents. | twelve antimicrobial agents were tested for use in axenic cultures of entamoeba histolytica and giardia lamblia. ten did not inhibit parasite growth at concentrations of at least two-fold above the effective antibacterial or antifungal levels. we have found the combination of piperacillin (500 micrograms/ml) plus amikacin (125 micrograms/ml) to be useful in axenization of a new strain of e. histolytica, eradication of multiply drug-resistant contaminants in cultures and prevention of bacterial g ... | 1985 | 2859675 |
| zymodemes expressing possible genetic exchange in entamoeba histolytica. | entamoeba histolytica was first seen by lösch (1875), and subsequently described by schaudinn (1903). since that time this organism has only been known to divide by binary fision. observations at molecular level now reveal possible genetic exchange in this species of amoeba. | 1985 | 2859677 |
| effects of cytochalasin b on na+ content and cell volume of entamoeba histolytica. | cells of entamoeba histolytica accumulated k+ and extruded na+ compared to the concentrations of those ions present in the growth medium. pinocytic activity, measured by the uptake of horseradish peroxidase of 125i-polyvinylpyrrolidone, was high (up to 0.3 ml/ml cells per h). upon addition of cytochalasin b, at a concentration (20 microm) that completely blocked pinocytosis, cells lost up to 40% of their na+ content within 90 min; k+ content was not affected or increased slightly compared to con ... | 1985 | 2859891 |
| entamoeba histolytica: purification of cathepsin b. | a cytotoxic cysteine proteinase with a molecular weight of 16,000 was isolated from axenically grown trophozoites of entamoeba histolytica. the enzyme was purified from frozen-thawed strain hm-1 by ion-exchange chromatography on deae-cellulose, organomercurial agarose affinity chromatography, and size-exclusion chromatography. the purified enzyme had proteinase activity that could be demonstrated on azocasein (ph 5), hemoglobin (ph 5), or carbobenzoxy-l-arginyl--l-arginyl-7-amino-4-trifluorometh ... | 1985 | 2860002 |
| vaccination of rabbits against entamoeba histolytica with aqueous suspensions of trehalose-dimycolate as the adjuvant. | rabbits were immunized with soluble entamoeba histolytica antigen with an aqueous suspension of trehalose-6,6'-dimycolate used as the adjuvant. induction of protective immunity in the immunized animals was demonstrated by enhanced humoral and cell-mediated immune responses and 100% survival after challenge. administration of soluble antigen only failed to induce a similar degree of protective immunity. trehalose-6,6'-dimycolate alone produced only a slight increase in nonspecific resistance. | 1985 | 2860068 |
| complement activation by antigenic fractions of entamoeba histolytica. | complement (c) activation induced by entamoeba histolytica in normal non-immune human serum was studied by testing in vitro the ability of different fractions of the trophozoites to cause c3 breakdown. whole trophozoites were found to activate both alternative and classical c pathways. the antibody-independent classical pathway (mgegta inhibitable) c activating capacity was found to greatly increase after disruption of the cell membrane by sonication. subsequent analysis after differential centr ... | 1985 | 2860628 |
| [invasive amebiasis (i): etiology, pathogenesis, epidemiology, and transmission. intestinal amebiasis and its complications]. | | 1985 | 2860702 |
| [invasive amebiasis (ii): extra-intestinal forms and complications. parasitological and serological diagnosis. treatment]. | | 1985 | 2860704 |
| [use of modified asami medium as a monoxenic culture medium for entamoeba histolytica]. | this is a report about the monoxenic cultivation of entamoeba histolytica in asami culture medium. the culture amebae serve as material for antigen preparation, for intrahepatic infections in golden hamsters and also for microscopical observation, s. figs. 2 and 3. | 1985 | 2860761 |
| cytologic demonstration of entamoeba histolytica using immunoperoxidase techniques. report of two cases. | an indirect immunoperoxidase technique to detect entamoeba histolytica in cell samples from patients suspected to have amebiasis is described. using a rat antiserum specific for e. histolytica, the organism was clearly identified both in smears and in cell blocks. this immunoperoxidase technique seems to offer great possibilities for a specific, accurate and rapid identification of amebic infestation in diagnostic cytology. | 1985 | 2860765 |
| use of a monoclonal antibody in an enzyme immunoassay for the detection of entamoeba histolytica in fecal specimens. | an indirect enzyme-linked immunosorbent assay (elisa) was developed for the detection of entamoeba histolytica in human feces, using both a monoclonal antibody and rabbit antisera. it detected from less than 1 to 57 trophozoites of 6 e. histolytica strains. stool specimens were positive by elisa in 18 of 22 (82%) patients with e. histolytica and in 3 of 186 (2%) of patients without demonstrable e. histolytica in their stools. the latter included one from a child living near an asymptomatic cyst ... | 1985 | 2860814 |
| secretory hormones of entamoeba histolytica. | watery diarrhoea as distinct from dysentery is occasionally seen in intestinal amoebiasis, suggesting a component of intestinal secretion. to study the pathogenesis of this watery diarrhoea, we evaluated the effect of lysates of entamoeba histolytica on active intestinal electrolyte transport using rabbit ileum and rat colon studied by the ussing chamber-voltage clamp technique. amoebic lysates added to the ileal and colonic mucosal surfaces did not alter electrolyte transport; in contrast, addi ... | 1985 | 2861068 |
| human amoebiasis: the interaction of lymphocyte surface-bound immune complexes and pmns impairs t cell proliferative responses to e. histolytica mitogen. | in some of the sera from patients with amoebiasis circulating immune complexes are present which are thought to interact with lymphoid cells, enabling them to elicit a burst of oxygen consumption in pmns. the intensity of chemiluminescence is related to the presence of c3+ and fc igg+ cells in the lymphoid cell suspensions employed. the generation and release of highly reactive oxygen derivatives from pmns impair t lymphocyte proliferative responses to the e. histolytica mitogen. the authors sug ... | 1985 | 2861159 |
| enzyme linked immunosorbent assay for serodiagnosis of amoebiasis in children. | | 1985 | 2861160 |
| chemotaxis by entamoeba histolytica. | a micropore membrane procedure to assay taxis by entamoeba histolytica is described and the results of studies of responses to a variety of soluble substances, bacteria, an rat colon washings using this procedure are reported. trophozoites migrated in blind well chambers through 8-micron pore size polycarbonate membranes but not nitrocellulose membranes up to 12 micron pore size. amoebae were attracted toward fresh axenic culture medium (tyi-s), an enzymatic hydrolysate of casein (trypticase), a ... | 1985 | 2861283 |
| incorporation and toxicity of 32p-orthophosphate and occurrence of polyphosphate in entamoeba trophozoites. | we explored the requirements of inorganic phosphate (pi), the incorporation of 32p-orthophosphate (32pi), and the occurrence of inorganic polyphosphate (polyp) in axenic entamoeba cultures. maximal population densities and growth rates of entamoeba histolytica trophozoites were attained in complete tp-s-1 medium. as 32pi concentration was increased in the medium, its own incorporation and the culture growth rate were progressively inhibited, especially in pi-deficient medium. polyp grains were f ... | 1985 | 2861284 |
| amebic colitis. preventing morbidity and mortality from fulminant disease. | uncomplicated amebic colitis is readily treated and has a mortality rate of less than 0.5%. complications necessitating surgical intervention develop in only 6% to 11% of patients with symptomatic disease. however, the mortality rate in these patients ranges from 40% to 100% and stems in part from delays in diagnosis and treatment. patients with known amebic colitis who show signs of systemic toxicity or of localized or generalized peritonitis are at high risk for complications; surgical consult ... | 1985 | 2861601 |
| infectious diarrhea. pathogenesis and risk factors. | our understanding of the pathogenesis of infectious, especially bacterial, diarrhea has increased dramatically. new etiologic agents, mechanisms, and diseases have become known. for example, escherichia coli serogroup o157 is now known to cause acute hemorrhagic colitis. also, e. coli serogroups that produce shiga toxin are recognized as etiologic agents in the hemolytic-uremic syndrome. the production of bacterial diarrhea has two major facets, bacterial-mucosal interaction and the induction of ... | 1985 | 2861742 |
| [anti-amebic effect of polyenic antibiotics]. | all-union research technological institute of antibiotics and medical enzymes, leningrad. institute of epidemiology, virology and medical parasitology, ministry of health of the armenian ssr. the effect of polyenic antibiotics made in the ussr on development of e. histolytica and e. moshkovski was studied. the following antibiotics were used: levorin and its derivatives, mycoheptin, amphotericin b, amphoglucamine and nystatin. the antibiotics were compared with emetine and metronidazole. some dr ... | 1985 | 2861787 |
| interactions between trophozoites of entamoeba histolytica and cells of the immune system. | the interaction between entamoeba histolytica trophozoites and cells of the immune system was studied in vitro. both immune as well as non-immune mononuclear cells (mnc) to some extent were capable of exerting a cytotoxic effect on e. histolytica trophozoites. incorporation of immune serum directed against the trophozoites in the reaction mixture resulted in a marked increase in the killing capacity of mnc. highest killing was observed (96.7 +/- 1.04%) with mnc obtained from animals immunized wi ... | 1985 | 2861805 |
| improved method of producing amoebic liver abscesses in hamsters for screening of systemically active amoebicides. | an improved method of producing hepatic amoebiasis in hamsters has been developed. a serially liver-passaged strain of entamoeba histolytica from experimentally-infected hamsters was used for infection. small pieces from the infected liver were examined for the presence of amoebae and contained approximately 20,000 active trophozoites per piece. such liver pieces, when used as an inoculum, produced infection in all of the animals. the method has been successfully employed for screening of known ... | 1985 | 2861806 |
| [frequency of enteropathogenic micro-organisms isolated in children with and without acute diarrhea]. | | 1985 | 2861829 |
| entamoeba histolytica: impedance measurements and cytotoxicity in the presence of bepridil, verapamil, and cytochalasin d. | entamoeba histolytica, and invasive enteric protozoa, kills mammalian target cells by sequential adherence and cytolytic events. using platinum plate electrodes with an alternating current source placed in a wheatstone bridge circuit, the impedance (resistance to ion flow) of a cell suspension of axenic amebae (strain hm1-imss) was measured. the impedance of the amebic cell suspension, expressed as resistivity (in ohm-cm), was significantly greater than the test solution and increased with decre ... | 1985 | 2862058 |
| differential lectin agglutinability of virulent and avirulent entamoeba histolytica strains. | | 1985 | 2862104 |
| electrophoretic isoenzymes of isolates of entamoeba histolytica before & after revival of their virulence. | | 1985 | 2862106 |
| rapid polymerization of entamoeba histolytica actin induced by interaction with target cells. | within 5 s of challenge of entamoeba histolytica trophozoites with red blood cells (rbc), attachment and deformation of target cells occurred at multiple sites on the amoeba surface. many trophozoite-target interfaces were outlined with a ring of polymerized amoeba actin, revealed by rhodamine-phalloidin staining of glutaraldehyde-fixed and triton-x 100-extracted cells. the beginnings of phagocytic pseudopods rimmed many targets. the phagocytic membrane and underlying actin network grew uniforml ... | 1985 | 2862217 |
| attempts to infect germfree nu/nu mice with entamoeba histolytica. | | 1985 | 2862642 |
| a seroepidemiological survey of amoebiasis in lucknow. | | 1985 | 2862724 |
| intestinal invasive amebiasis: an experimental model in rodents using axenic or monoxenic strains of entamoeba histolytica. | a new experimental model for the production of early stages of invasive intestinal amebiasis in hamsters or guinea pigs with axenic or monoxenic cultures of entamoeba histolytica of the hm1:imss strain is reported. the model is called the washed-closed cecal loop, because it involves the washing out of the cecum contents of conventionally raised animals and the formation of a closed loop, into which amebas are inoculated. colonization occurred in all inoculated animals. after 48 hr, macroscopic ... | 1985 | 2862801 |
| [etiology of acute infantile gastroenteritis in gabon]. | rotaviruses are the main etiology of acute diarrhoeas in gabonese children (11 to 30% according to age). salmonellae (11.4%), shigellae (7.1%) and e. histolytica (7.1%), isolated or associated with enterobacteria, e. coli (3%), giardia and strongyloides stercoralis (1.4%), yersinia enterocolitica (1%) and balantidium coli (0.5%) were also found, without cholera. | 1985 | 2863005 |
| [endocytosis of liposomes by entamoeba histolytica and transport of encapsulated molecules toward the cytoplasm]. | we have examined the interaction of a highly phagocytosing cell: entamoeba histolytica with liposomes of different lipid compositions, and followed, by a semi-quantitative method, the intracellular fate of the entrapped molecules. liposomes containing a small molecule, 6-carboxyfluorescein, are first phagocytosed. then the encapsulated compound migrates from the vacuoles to the cytoplasm. liposomes containing macromolecular substances, such as fluorescent albumin or ferritin, are also phagocytos ... | 1985 | 2863146 |
| in vitro and in vivo interaction between trophozoites of entamoeba histolytica and gerbil lymphoid cells. | the in vitro and in vivo antiamoebic cytotoxic effects of peritoneal exudate cells and mesenteric lymph node lymphocytes of gerbils with cecal amoebiasis or those immunized with amoebic extract were investigated. a differential effect of the lymphoid cells against trophozoites of nonpathogenic and pathogenic strains of entamoeba histolytica was observed. nonpathogenic amoebae were more susceptible to killing by lymphoid cells than pathogenic amoebae in vitro and in vivo in infected or immunized ... | 1985 | 2863217 |
| interaction of human leukocytes and entamoeba histolytica. killing of virulent amebae by the activated macrophage. | capable effector mechanisms in the human immune response against the cytolytic, protozoan parasite entamoeba histolytica have not been described. to identify a competent human effector cell, we studied the in vitro interactions of normal human polymorphonuclear neutrophils, peripheral blood mononuclear cells (pbmc), monocytes (mc), and mc-derived macrophages with virulent axenic amebae (strain hmi-imss). amebae killed neutrophils, pbmc, mc, and mc-derived macrophages (p less than 0.001), without ... | 1985 | 2863284 |
| phospholipase a enzymes of entamoeba histolytica: description and subcellular localization. | cytolysis of target cells by entamoeba histolytica is a rapid, contact-dependent event that appears to involve calcium and amebic phospholipase a enzymes. there are two phospholipase a enzymes of e. histolytica: one calcium independent and optimally active at an acid ph, and a second calcium dependent and most active at an alkaline ph. the amebic calcium-dependent enzyme was inhibited by pharmacological antagonists that have been shown to reduce cytolysis of target cells by intact amebae: phosph ... | 1985 | 2863316 |
| effect of antagonists of calcium and phospholipase a on the cytopathogenicity of entamoeba histolytica. | the in vitro mechanisms by which entamoeba histolytica trophozoites lyse target chinese hamster ovary (cho) cells were examined. calcium chelators ethylenediaminetetraacetate and ethyleneglycol bis (beta-aminoethyl ether)-n,n'-tetraacetate (10 mm) inhibited amebic cytolysis of target cho cells (p less than .01). a putative antagonist of intracellular calcium flux, 8-(n,n-diethylamino)octyl-3,4,5-trimethoxybenzoate (tmb-8; greater than or equal to 250 microm), inhibited amebic adherence and cytol ... | 1985 | 2863317 |
| [native entamoeba histolytica infections]. | | 1985 | 2863438 |
| effect of lanosterol on the susceptibility of mastomys natalensis to entamoeba histolytica infection. | | 1985 | 2863883 |
| entamoeba histolytica: electrophoretic isoenzyme patterns of strains and their virulence in the cecum of gerbils (meriones unguiculatus). | six strains of entamoeba histolytica isolated from humans were characterized by their electrophoretic isoenzyme patterns and by virulence in the cecum of mongolian gerbils (meriones unguiculatus). amebae from symptomatic cases of amebiasis were found to belong to zymodeme group ii, whereas strains isolated from asymptomatic infections belonged to zymodeme groups i or v. strains recently isolated from symptomatic cases of amebiasis were highly invasive in the cecum of gerbils. the most prominent ... | 1985 | 2863988 |
| [immunologic characterization of a monoclonal antibody specific for e. histolytica]. | | 1985 | 2864744 |
| detection of entamoeba histolytica in the pus of amoebic liver abscess by immunofluorescent technique. | | 1985 | 2865204 |
| activation of the alternative and classical complement pathways by entamoeba histolytica. | entamoeba histolytica hm1 supported the activation of human alternative and classical complement pathways in the absence of ameba-reactive antibodies. nonimmune serum depleted of c1q and factor d (nhs s c1q + d) and reconstituted with c1q was able to specifically deposit c3b onto trophozoites and produce lysis. this activity was not modified by the absorption of serum on e. histolytica. serum depleted of factor b allowed c3b binding to amebae. serum devoid of c4 effected only small amounts of c3 ... | 1985 | 2865212 |
| a dot enzyme-linked immunosorbent assay for detection of antibodies against entamoeba histolytica. | a visual micro-dot enzyme-linked immunosorbent assay (elisa) based on detection of antibodies against soluble antigens of axenically grown cultures of entamoeba histolytica is described. the antigen was spotted on a nitrocellulose sheet, the unsaturated sites blocked with bovine serum albumin (bsa) and incubated with 3-fold dilutions of patient sera followed by incubation with protein a conjugated to peroxidase. enzymic activity was evidenced using the substrate 4-chloro-1-naphthol. a positive r ... | 1985 | 2865313 |
| entamoeba histolytica: lymphoreticular changes in gerbils (meriones unguiculatus) with experimentally induced cecal amebiasis. | antibody responses and changes in the lymphoreticular tissues of gerbils with experimental cecal amebiasis were studied from 5 to 60 days pi. changes in the cecum consisted of lymphoid follicle hyperplasia and depletion of lymphocytes, followed by follicle atrophy and histiocytosis. mesenteric lymphadenopathy, and histologic alterations in the lymph nodes paralleled the progressive development of amebic cecal lesions. early in the infection (5 to 10 days pi) mesenteric lymph nodes showed cortica ... | 1985 | 2865345 |
| purine salvage in entamoeba histolytica. | pulse-labeling of the nucleotide pool in entamoeba histolytica with radioactive precursors, and subsequent high performance liquid chromatographic (hplc) analysis of the radiolabeled nucleotides, indicate that e. histolytica is incapable of de novo synthesis of purine nucleotides. hypoxanthine, inosine and xanthine could not be converted to nucleotides in e. histolytica, which suggests the absence of interconversion between adenine nucleotides and guanine nucleotides through formation of imp. ad ... | 1985 | 2865346 |
| experimental infection of rhesus monkeys with entamoeba histolytica mimics human infection. | experimental infection of entamoeba histolytica was successfully established in rhesus monkeys (macaca mulatta). parasites of proven pathogenicity maintained through liver passage in hamsters were used in this study. laparotomized monkeys were inoculated intracecally/intrahepatically with trophozoites, or by oral administration of cysts (2000 cysts/ml). the cysts were isolated from an infected human stool sample. formation of typical amebic lesions in the inoculated tissues along with alteration ... | 1985 | 2865401 |
| [entamoeba histolytica and other intestinal parasites in an institution for the mentally retarded]. | | 1985 | 2865836 |
| entamoeba histolytica, antigens and amoebiasis. | | 1985 | 2866068 |
| inhibition of human monocyte locomotion by products of axenically grown e. histolytica. | the supernatant fluid of axenically grown e. histolytica inhibits chemotaxis, chemokinesis and random mobility of human mononuclear phagocytes (mp) as measured in boyden chambers. human polymorphonuclear phagocytes (pmn) locomotion is apparently unaffected. the factor was found in comparable amounts in the supernatant fluid of axenic cultures of four e. histolytica strains that differed in their human pathogenicity and virulence, as well as in two entamoebas non-pathogenic for man. this dialysab ... | 1985 | 2866481 |
| [culture methods in the diagnosis of intestinal parasitic protozoans in man, particularly entamoeba histolytica]. | | 1985 | 2866849 |
| multilayer-enzyme linked immunosorbent assay (ml-elisa) for detection of entamoeba histolytica trophozoite coproantigen. | multilayer elisa (ml-elisa) method for entamoeba histolytica trophozoite coproantigen was developed for diagnosis of amoebiasis and its usefulness vis-a-vis microscopic examination analysed for paired specimens from 87 patients from lucknow and hyderabad, india. all the stool specimens positive for trophozoites were also found to be positive in ml-elisa. out of a total 32 samples positive for cysts in microscopy 21 (65.62%) gave positive reactions for coproantigen. results on 26 samples drawn fr ... | 1985 | 2867033 |
| which amoebae are harmful? | | 1985 | 2867035 |
| susceptibility of entamoeba histolytica to oxidants. | the effect of hydrogen peroxide and hypochlorite on culture forms of entamoeba histolytica trophozoites was examined by using two strains of e. histolytica, virulent (ip:0682:1) and nonvirulent (dkb). the amoebae were incubated with various concentrations of hydrogen peroxide and hypochlorite, and their viability was determined at different times after incubation. when the viability of the virulent and nonvirulent strains was compared to different oxidant strengths, it became apparent that the v ... | 1986 | 2867045 |
| acanthamoeba, an opportunistic microorganism: a review. | granulomatous amebic encephalitis due to acanthamoeba spp. usually occurs in chronically ill and debilitated individuals. some of these patients may have received immunosuppressive therapy. another infection due to acanthamoeba spp. has been corneal ulcerations which usually occur after minimal trauma to the corneal epithelium (1). in contrast, primary amebic meningoencephalitis due to naegleria fowleri usually occurs in healthy, young individuals with a history of swimming in heated swimming po ... | 1985 | 2867047 |
| [short-term treatment of protozoonoses with tiberal]. | | 1985 | 2867648 |