| the protective effect of selenium inorganic forms against cadmium and silver toxicity in mycelia of pleurotus ostreatus. | the effect of two inorganic selenium forms has been investigated in the mycelia of pleurotus ostreatus exposed to cadmium and silver salts in the shaken cultures. the degree of toxicity was assessed by the determination of malondialdehyde (mda; a common biomarker of lipid peroxidation). the mycelia were exposed to one element form (up to 5 mg l(-1)) and also to the following combinations: cadmium(ii) + selenium(iv); cadmium(ii) + selenium(vi); silver(i) + selenium(iv); silver(i) + selenium(vi). ... | 2007 | 17509851 |
| production of lignocellulose-degrading enzymes and changes in soil bacterial communities during the growth of pleurotus ostreatus in soil with different carbon content. | the extracellular enzyme activity and changes in soil bacterial community during the growth of the ligninolytic fungus pleurotus ostreatus were determined in nonsterile soil with low and high available carbon content. in soil with p. ostreatus, the activity of ligninolytic enzymes laccase and mn-peroxidase was several orders of magnitude higher than in soil without the fungus. addition of lignocellulose to soil increased the activity of cellulolytic fungi and the production of mn-peroxidase by p ... | 2006 | 17455795 |
| structural characterization of heterodimeric laccases from pleurotus ostreatus. | the subfamily of poxa3 laccase isoenzymes produced by the fungus pleurotus ostreatus has been characterized as an example of the complexity and heterogeneity of fungal isoenzyme patterns. two isoenzymes, poxa3a and poxa3b, were previously purified, exhibiting an unusual heterodimeric structure composed of a large (67 kda) and a small (18 or 16 kda) subunit. a unique gene encodes the large subunit of both poxa3a and poxa3b, but alternative splicing produces two variants--differing for an insertio ... | 2007 | 17429621 |
| lentinus edodes and pleurotus species lignocellulolytic enzymes activity in submerged and solid-state fermentation of lignocellulosic wastes of different composition. | lentinus edodes and pleurotus species from various origins were compared for the first time for their ability to produce lignocellulolytic enzyme in solid-state (ssf) and submerged (sf) fermentation of various plant raw material. fungi cultivation in identical culture conditions revealed wide differences among both species and strains of the same species. the yields of cmcase (62.3uml(-1)), xylanase (84.1 u ml(-1)), fpa (5.9 u ml(-1)), and laccase (4103 ul(-1)) are the best so far obtained with ... | 2008 | 17350827 |
| oyster mushroom reduced blood glucose and cholesterol in diabetic subjects. | it has been postulated that mushroom has beneficial effect of lowering blood glucose and cholesterol in diabetic subjects. the literature so far searched and found that there was no published data in this regard. this study was undertaken to assess the effect of reducing blood glucose, cholesterol and triglycerides in diabetic patients. additionally, this study addressed whether there was any hepatic and renal toxicity of mushroom. this clinical investigation was conducted in birdem hospital fro ... | 2007 | 17344789 |
| effects of fermented oyster mushroom ( pleurotus ostreats) by-product supplementation on growth performance, blood parameters and meat quality in finishing berkshire pigs. | the objective of the present study was to investigate the effects of fermented oyster mushroom (pleurotus ostreats) by-production (fomp) supplementation on the growth performance, blood parameters, carcass traits and meat quality in finishing berkshire pigs. fomp was made by mixing oyster mushroom by-production with rice bran and barley bran and this mixture was fermented for 60 days. the experimental diets were 0, 3, 5 and 7% of fomp added to c, t1, t2 and t3 in the basis diet for 7 weeks. aver ... | 2007 | 22444296 |
| occupational asthma due to spores of pleurotus ostreatus. | | 2007 | 17298434 |
| feasibility of using olive mill effluent (ome) as a wetting agent during the cultivation of oyster mushroom, pleurotus ostreatus, on wheat straw. | in this study, cultivation of oyster mushroom, pleurotus ostreatus, on wheat straw substrate containing tap water and olive mill effluent (ome) mixture containing varying volume of ome was studied in order to investigate the feasibility of using ome as an alternative wetting agent and ome's impact on some fundamental food quality characteristics of mushrooms. time period for mycelial colonization, primordium initiation and first harvest were comparatively evaluated with the control group. it was ... | 2008 | 17239585 |
| effect of various substrates on the growth and quality of mushrooms. | the effect of different biowastes such as paddy straw, sorghum straw, sugarcane molasses, saw dust and paper waste on the growth and biochemical constituents of oyster mushroom (pleurotus florida) was studied. favourable conditions were created to attain the maximum yield of mushrooms. the results reveled that mushroom growth was better in paddy straw followed by sugarcane molasses and least in wood saw dust and paper waste. the growth of mushrooms may be coincided with type of substrates used t ... | 2007 | 19070009 |
| study of biodegradation products from azo dyes in fungal degradation by capillary electrophoresis/electrospray mass spectrometry. | biodegradation products from four model sulfonated azo dyes orange ii, acid orange 8, food yellow 3, and 4-[(4-hydroxyphenyl)azo]-benzenesulfonic acid, sodium salt (4haba), during fungal degradation were determined by capillary electrophoresis coupled with ion trap mass spectrometry (ce-ms) with electrospray ionization and a coaxial sheath flow interface. the development and optimization of this analytical method including the sheath liquid composition and flow rate, nebulizing gas flow rate, ca ... | 2007 | 17126346 |
| fumigant activity of plant essential oils and components from schizonepeta tenuifolia against lycoriella ingenua (diptera: sciaridae). | plant essential oils from 21 plant species were tested for their insecticidal activities against larvae of lycoriella ingenua dufour (diptera: sciaridae) by using a fumigation bioassay. good insecticidal activity against larvae of l. ingenua was achieved with essential oils of acorus gramineus solander, schizonepeta tenuifolia briquet, and zanthoxylum piperitum de candolle at 25 microg/ml air. s. tenuifolia oil showed the most potent insecticidal activity among the plant essential oils. at 12.5 ... | 2006 | 17066804 |
| two new species of trichoderma associated with green mold of oyster mushroom cultivation in korea. | this paper describes and illustrates two new species, trichoderma pleurotum and t. pleuroticola, associated with green mold disease of oyster mushroom in korea. | 2006 | 24039481 |
| steroid structural requirements for interaction of ostreolysin, a lipid-raft binding cytolysin, with lipid monolayers and bilayers. | ostreolysin, a cytolytic protein from the edible oyster mushroom (pleurotus ostreatus), recognizes and binds specifically to membrane domains enriched in cholesterol and sphingomyelin (or saturated phosphatidylcholine). these events, leading to permeabilization of the membrane, suggest that a cholesterol-rich liquid-ordered membrane phase, which is characteristic of lipid rafts, could be its possible binding site. in this work, we present effects of ostreolysin on membranes containing various st ... | 2006 | 16857161 |
| exclusive overproduction of recombinant versatile peroxidase mnp2 by genetically modified white rot fungus, pleurotus ostreatus. | by combining a homologous recombinant gene expression system and optimization of the culture conditions, hyper overproduction of pleurtous ostreatus mnp2 was achieved. genetically modified p. ostreatus strains with the recombinant mnp2 sequence under the control of sdi1 expression signals, were subjected to agitated culture using media supplemented with wheat bran or its hot-water extract. the best result, whereby 7300 u/l of mnp was produced by a recombinant strain tm2-18, indicated that more t ... | 2006 | 16820241 |
| influence of dimethyl sulfoxide on extracellular enzyme production by pleurotus ostreatus. | dimethyl sulfoxide (dmso) is commonly used as a co-solvent to dissolve poorly water-soluble biologically active agents to assess their biological activities such as for enzyme induction. the question addressed was whether dmso can be assumed to be an inert co-solvent. the influence of dmso on the production of extracellular enzymes by pleurotus ostreatus was investigated. dmso functioned as either an inducer or a repressor, depending on the enzyme studied. the production of laccase and endo-1,4- ... | 2006 | 16642304 |
| se-enriched mycelia of pleurotus ostreatus: distribution of selenium in cell walls and cell membranes/cytosol. | the incorporation of se to fungi has been studied, focusing on element distribution among different cellular compartments and, in particular, polysaccharide structures contained in cell walls. se-enriched mycelia of pleurotus ostreatus were obtained in submerged cultures. the incorporation of selenium from the growth medium to mycelia was observed with the relative distribution between cytosol plus cell membranes fraction (ccm) and cell walls fraction (cw) of about 44 and 56%, respectively. ccm ... | 2006 | 16637705 |
| lovastatin production by pleurotus ostreatus: effects of the c:n ratio. | the types of carbon source and nitrogen source used as well as the c:n ratio in the medium influenced lovastatin production by pleurotus ostreatus. the maximum value of the lovastatin yield was obtained in a medium that contained organic nitrogen. | 2013 | 16610224 |
| growth of pleurotus ostreatus on wheat straw and wheat-grain-based media: biochemical aspects and preparation of mushroom inoculum. | mycelial growth, intracellular activity of proteases, laccases and beta-1,3-glucanases, and cytoplasmic protein were evaluated in the vegetative phase of pleurotus ostreatus grown on wheat straw and in wheat-grain-based media in petri dishes and in bottles. the productivity of the wheat straw and wheat-grain-based spawn in cylindrical polyethylene bags containing 5 kg of chopped straw was also determined. we observed high activity of proteases and high content of intracellular protein in culture ... | 2006 | 16586105 |
| genetic linkage map and expression analysis of genes expressed in the lamellae of the edible basidiomycete pleurotus ostreatus. | pleurotus ostreatus is an industrially cultivated basidiomycete with nutritional and environmental applications. its genome contains 35 mbp organized in 11 chromosomes. there is currently available a genetic linkage map based predominantly on anonymous molecular markers complemented with the mapping of qtls controlling growth rate and industrial productivity. to increase the saturation of the existing linkage maps, we have identified and mapped 82 genes expressed in the lamellae. their manual an ... | 2006 | 16531085 |
| [yellow laccase from the fungus pleurotus ostreatus d1: purification and characterization]. | yellow laccase was isolated from a solid-phase culture of the fungus pleurotus ostreatus d1 and characterized. it is a copper-containing enzyme with molecular weight 64 kda. its absorption spectrum lacks the maximum at 610 nm, characteristic of fungal laccases and corresponding to type i copper atom. the optimum ph values for the enzyme were determined. they proved to be: 7.0 for syringaldazine, 8.0 for pyrocatechol, and 4.0 for 2,2'-azine-bis-(3-ethylbenzothiazoline-6-sulfonate and 2,6-dimethox ... | 2015 | 16521579 |
| an aqueous polysaccharide extract from the edible mushroom pleurotus ostreatus induces anti-proliferative and pro-apoptotic effects on ht-29 colon cancer cells. | anti-proliferative and pro-apoptotic activities of fractions of pleurotus ostreatus were examined using ht-29 colon cancer cells in vitro. a hot-water-soluble fraction of the mycelium of the liquid cultured mushroom was partially isolated and chemically characterized as a low-molecular-weight alpha-glucan. ht-29 cells were exposed to the different isolates and significant inhibition of proliferation was obtained in a dose-dependent manner. proliferation inhibition was shown to be the result of a ... | 2006 | 16413114 |
| structure selectivity in degradation and translocation of polychlorinated biphenyls (delor 103) with a pleurotus ostreatus (oyster mushroom) culture. | the biodegradation of polychlorinated biphenyls (pcbs), formerly applied in technical mixtures called delor 103, was realized by the white rot fungus pleurotus ostreatus. besides the efficiency of degradation the translocation of pcbs into the fruit bodies grown during cultivation on straw were of special interest. the selectivity of pcbs degradation is discussed on the basis of structural features of the individual congeners. the most important structural factors affecting the degradation effic ... | 2005 | 16291407 |
| [expression of a laccase gene from pleurotus ostreatus in pichia pastoris and characterization of the recombinant enzyme]. | a pleuotus ostreatus laccase gene was cloned by rt-pcr and designated as lccpol. its sequence was submitted to genbank with the accession number ay450404 obtained. the open reading frame was transformed into three pichia pastoris strains gs115, km71 and smd1168, respectively, under control of the aox1 promoter by using the vector phbm906. lccpo1 can be expressed by all three p. pastoris recombinant strains. three different strategies for shake-flask cultures were compared: (1) (25 degrees c, 1.0 ... | 2005 | 16245886 |
| response surface analysis of solid state growth of pleurotus ostreatus mycelia utilizing whey permeate. | a novel approach to utilizing whey permeate, the cultivation of mycelia of the edible mushroom pleurotus ostreatus, is introduced. response surface analysis (rsa) was successfully applied to determine the combination of substrate concentration, temperature and ph that would result in a maximal mycelial extension rate under solid state cultivation. the conditions to maximize the mycelial extension rate were predicted to be 44 g lactose l(-1), ph 6.0 and 24.2 degrees c. subsequent verification of ... | 2005 | 16245171 |
| laccase-mediated remazol brilliant blue r decolorization in a fixed-bed bioreactor. | a crude laccase mixture preparation from pleurotus ostreatus cultures supplemented with copper and ferulic acid was used to decolorize the anthraquinonic dye remazol brilliant blue r (rbbr). performance of this enzymatic system was tested, and a maximum of 70% decolorization was achievable under optimal conditions. the crude preparation was immobilized by entrapment in copper alginate beads attaining 65% yield of laccase activity. stability of the immobilized laccases was remarkably increased in ... | 2015 | 16209547 |
| molecular breeding of white rot fungus pleurotus ostreatus by homologous expression of its versatile peroxidase mnp2. | using a dna-mediated transformation technique, a molecular breeding approach to isolate pleurotus ostreatus strains with enhanced productivity of its versatile peroxidase mnp2 was conducted. a recombinant mnp2 construct under the control of p. ostreatus sdi1 expression signals was introduced into the wild-type p. ostreatus strain by cotransformation with a carboxin-resistant marker plasmid. a total of 32 transformants containing the recombinant mnp2 sequence were isolated in a screening with spe ... | 2006 | 16163536 |
| effect of ph on the pore forming activity and conformational stability of ostreolysin, a lipid raft-binding protein from the edible mushroom pleurotus ostreatus. | ostreolysin, a pore-forming protein from the edible oyster mushroom (pleurotus ostreatus), is a member of the aegerolysin protein family, a novel group of small acidic proteins found in bacteria, molds, mushrooms, and plants. it binds to lipid rafts and interacts specifically with cholesterol-rich lipid domains. in this study, ostreolysin was classified as a single-domain all-beta-structured protein on the basis of cdna sequencing. ph-induced and thermally induced unfolding of ostreolysin was st ... | 2005 | 16101298 |
| a carbon-carbon-coupled dimeric bergenin derivative biotransformed by pleurotus ostreatus. | a novel c-c-coupled dimer derivative of bergenin was produced by the biotransformation of cultured mycelia of white rot fungus pleurotus ostreatus. its structure was elucidated by detailed spectroscopic analysis. | 2005 | 15993599 |
| pleurostrin, an antifungal peptide from the oyster mushroom. | a 7kda peptide, with inhibitory activity on mycelial growth in the fungi fusaerium oxysporum, mycosphaerella arachidicola and physalospora piricola, was isolated from fresh fruiting bodies of the oyster mushroom. the isolation procedure entailed extraction with an aqueous buffer, ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel and gel filtration by fast protein liquid chromatography on superdex 75. the protein was unadsorbed on deae-cellulose and adsor ... | 2005 | 15941607 |
| [comparative analysis of pleurotus ostreatus natural isolates]. | a comparative analysis is performed of the polymorphism of the pleurotus ostreatus (fr.) kumm naturally occurring strains isolated from the natural substrates found in two geographically remote russian natural preserves, the central arboreal biosphere tver state preserve (cabtsp) and the moscow state university zvenigorod biological station (zbs, moscow oblast), and within the city of moscow. the results of the frequency analysis for the isozyme loci alleles and for the sexual and vegetative inc ... | 2006 | 15938400 |
| degradation of lignocellulose by pleurotus ostreatus in the presence of copper, manganese, lead and zinc. | pleurotus ostreatus produces the cellulolytic and hemicellulolytic enzymes endo-1,4-beta-glucanase, exo-1,4-beta-glucanase, 1,4-beta-glucosidase, endo-1,4-beta-xylanase, 1,4-beta-xylosidase, endo-1,4-beta-mannanase and 1,4-beta-mannosidase and ligninolytic enzymes mn-peroxidase and laccase during growth on wheat straw in the presence and absence of cu, mn, pb, and zn. this is the first report concerning endo-1,4-beta-mannanase in p. ostreatus. although the concentrations of trace metals in wheat ... | 2013 | 15921894 |
| 3-o-alkyl-d-glucose derivatives induce fruit bodies of pleurotus ostreatus. | amphipathic glucose derivatives 3-o-octyl- and 3-o-decyl-d-glucose were synthesized, and the ability to induce fruit bodies on pleurotus ostreatus was examined. surfactants such as chaps, chapso, mega-8, mega-9, and mega-10 were also assayed. the result was that both glucose derivatives could stimulate fruiting on p. ostreatus, while none of the surfactants assayed in this study stimulated the fruiting of p. ostreatus. these results suggest that sugar moiety is necessary for amphipathic compound ... | 2005 | 15912955 |
| [137cs distribution and accumulation in the tissues of cultivated fungi (pleurotus ostreatus)]. | the authors examined 137cs accumulation and distribution in different structures and tissues of pleurotus ostreatus cultivated under laboratory conditions. the fungi were shown to concentrate 137cs. a higher concentrations of the radionuclides in the fungi compared to their substrate is manifested at the first stages of the fruit body formation, the maximum content of 137cs is accumulated by fungi in the middle of bearing stage. the fungus tissues are different by their accumulative capacity as ... | 2016 | 15906870 |
| isolation of genes differentially expressed during the fruit body development of pleurotus ostreatus by differential display of rapd. | to analyze genes involved in fruit body development of pleurotus ostreatus, mrnas from three different developmental stages: i.e., vegetative mycelium, primordium, and mature fruit body, were isolated and reverse-transcribed to cdnas. one hundred and twenty random pcr amplifications were performed with the cdnas, which generated 382, 394, 393 cdna fragments from each developmental stage. from these fragments, four cdna clones specifically expressed in primordium or mature fruit body were detecte ... | 2005 | 15899417 |
| interference of laccase in determination of cellobiose dehydrogenase activity of pleurotus ostreatus (florida) using dichlorophenol indophenol as the electron acceptor. | pleurotus ostreatus (florida), itcc 3308 produces approximately 9.0 u/ml extracellular cellobiose dehydrogenase (cdh) in cellulose medium after 7 days of growth. however, no activity could be detected if the assay was done with cellobiose as the substrate and 2,6-dichlorophenol indophenol (dpip) as the electron acceptor in absence of any laccase inhibitor. kinetic study showed that v(max)/k(m) value was very high for rdpip (reduced 2,6-dichlrophenol indophenol) oxidation by laccase. oxygen consu ... | 2005 | 15812859 |
| [comparative study on the productivity of strains of pleurotus spp. in commercial cultivation]. | this paper describes the commercial production of two strains of pleurotus pulmonarius, selected in the laboratory for their rapid mycelial development and high production of basidiomata, and one commercial strain of pleurotus ostreatus. substrate preparation, impact of pathogens and environmental conditions necessary for the production and quality of the fruiting bodies required are discussed. | 2000 | 15762809 |
| changes of enzyme activities and compositions of abnormal fruiting bodies grown under artificial environmental conditions in pleurotus ostreatus. | this study investigated the biochemical changes of abnormal fruiting bodies grown under artificial environmental conditions in p. ostreatus. abnormal mushroom growth during cultivation damages the production of good quality mushroom. this study showed that different environmental conditions produced morphological changes in the fruiting bodies of p. ostreatus. the fruiting bodies with morphological changes were collected and examined for differences in biochemical properties, enzyme activities, ... | 2005 | 24049471 |
| [use of coffee grounds for production of pleurotus ostreatus (jacq.:fr.) kummer]. | studies were carried out to screen the industrial strain hk35 of pleurotus ostreatus for its ability to develop fruiting bodies in solid state cultivation using several substrates containing 17.8 to 55% coffee grounds. our results showed that only 55% of coffee grounds was used in the substrate without detecting changes in fruiting body or on its biological efficiency of production. the chemical analysis of the caffeine in the substrate showed that this compound decreased about 59% of the myceli ... | 2004 | 15709800 |
| determination of biodegradation products from sulfonated dyes by pleurotus ostreatususing capillary electrophoresis coupled with mass spectrometry. | microbial treatment of environmental pollutants including dyes with white rot fungi has received wide attention as a potential alternative for conventional methods in wastewater treatment. the degradation products from dyes and mechanism underlying fungal degradation of dyes is desirable to be understood. capillary electrophoresis coupled with mass spectrometry (ce-ms) was used in this study to determine biodegradation products of 4-[(4-hydroxyphenyl)azo]-benzenesulfonic acid, sodium salt (4haba ... | 2005 | 15685423 |
| complete nucleotide sequence and genome organization of a dsrna partitivirus infecting pleurotus ostreatus. | the nucleotide sequences of the genomic dsrna mycovirus infecting pleurotus ostreatus (p. ostreatus virus 1; pov1) were determined and compared to the sequences of the other mycoviruses belonging to partitiviruses and totivirues. pov1 dsrna-1 and dsrna-2 had genomes of 2296 and 2223 nucleotides, respectively. the purified virus preparations contained isometric particles of 28-30 nm in diameter, and also the same two dsrnas were isolated from purified virus preparations. the sequences of pov1 dsr ... | 2005 | 15681061 |
| biodegradation of lindane by pleurotus ostreatus via central composite design. | the degradation of lindane was studied in liquid-agitated cultures using a commercial strain of the fungus pleurotus ostreatus as the biodegrading organism. the biodegradation was accomplished with the action of extracellular oxidative enzymes, produced by the fungus to decompose woody substrates. enzyme activities of manganese peroxidase and laccase were measured in a liquid mineral medium. an orthogonal central composite design of experiments was used to construct second-order response surface ... | 2005 | 15661282 |
| mn(2+) is dispensable for the production of active mnp2 by pleurotus ostreatus. | the regulation mechanism for expression of versatile peroxidase mnp2 by the basidiomycete fungus pleurotus ostreatus was examined using chemically defined synthetic media. expression of mnp2 was down-regulated at the transcription level by nutrient nitrogen, e.g., nh(4)(+), arginine or urea. as is often the case with other fungal manganese peroxidases, active mnp2 was not detected when mn(2+) was omitted from the culture, while mnp2 transcription was barely affected by mn(2+). however, mn(2+) ca ... | 2005 | 15649426 |
| mass spectrometry and database search in the analysis of proteins from the fungus pleurotus ostreatus. | tandem mass spectrometry is a method of choice for rapid analysis in proteomics. identification and characterization of proteins from organisms with sequenced genomes is today a routine procedure as will be identification of proteins from organisms with unsequenced genomes with new developing tools. here, we report the use of isotopic labeling with electrospray ionisation (esi)-tandem mass spectrometry for de novo sequencing in combination with database search taking advantage of different progr ... | 2005 | 15602771 |
| [interspecific genetic variability of the oyster mushroom pleurotus ostreatus as revealed by allozyme gene analysis]. | allozyme variation in natural populations of basidiomycetes fungus pleurotus ostreatus (88 individuals) from three regions of central russia was studied. the species was shown to have 92.86% of polymorphic allozyme loci and expected heterozygosity he = 0.49. the mean number of alleles per locus was 3.5. the genetic differences among populations were supported by f-statistics (fst = 0.750). the low level of inbreeding (fis = 0.018) suggests that the p. ostreatus populations are panmictic, and the ... | 2004 | 15523846 |
| [treatment of coloured industrial effluents with pleurotus spp]. | the decolouration of fermentation residues (vinasse) and liquid extract of coffee pulp by the mushroom pleurotus ostreatus was studied in addition to laccase activity. the fungus was inhibited in both residues when they remained undiluted. in submerged cultivation on wastewaters a good production of biomass (14.8 g/l for vinasse and 5.4 g/l for extract of coffee pulp) and also laccase activity (14.1 u/ml for vinasse and 3.0 u/ml for extract of coffee pulp) up to the 10 days of fermentation was o ... | 2003 | 15456356 |
| the mannitol cycle in pleurotus ostreatus (florida). | in mushroom, presence of the mannitol cycle has not been reported so far although the polyol is supposed to be generated by the reduction of fructose by mannitol dehydrogenase. this study submits evidence for the presence of the mannitol cycle in pleurotus ostreatus. the key enzyme of the cycle, mannitol-1-phosphate dehydrogenase (m1pdh), was present appreciably in all the developmental stages of the mushroom. however, the enzyme level dropped significantly at the onset of sporulation. the prese ... | 2004 | 15251212 |
| decolorization of orange g by pleurotus ostreatus monokaryotic isolates with different laccase activity. | the effect of enhanced laccase (lac) activity (obtained after copper addition to cultivation media) on decolorization of azo dye orange g in two basidiospore-derived monokaryotic isolates of pleurotus ostreatus was determined. the high lac-producing isolate efficiently decolorized orange g. the low-producing isolate showed only poor decolorization ability during cultivation in liquid medium and no decolorization on agar plates containing orange g after a 25-d growth. a substantial enhancement of ... | 2003 | 15058191 |
| fungal upgrading of wheat straw for straw-thermoplastics production. | combining biologic pretreatment with storage is an innovative approach for improving feedstock characteristics and cost, but the magnitude of responses of such systems to upsets is unknown. unsterile wheat straw stems were upgraded for 12 wk with pleurotus ostreatus at constant temperature to estimate the variation in final compositions with variations in initial moisture and inoculum. degradation rates and conversions increased with both moisture and inoculum. a regression analysis indicated th ... | 2004 | 15054197 |
| structure of gene coding for the fruit body-specific hydrophobin fbh1 of the edible basidiomycete pleurotus ostreatus. | two fruit body-specific hydrophobins (fbh1 and poh1) have been identified in two different strains of the edible basidiomycete pleurotus ostreatus. comparison of their nucleotide and amino acid sequences yielded similarity values (59% and 66%, respectively) smaller than those found for alleles of the same hydrophobin gene but higher than those found for different hydrophobin genes in p. ostreatus var. florida (peñas et al 2002). in this paper, we have addressed the question of fbh1 and poh1 alle ... | 2010 | 21148831 |
| immunofluorescence microscopy of the microtubule cytoskeleton during conjugate division in the dikaryon pleurotus ostreatus n001. | fluorescence microscopy was used to describe the distribution of nuclei and the organization of the microtubule network in hyphae of pleurotus ostreatus. dikaryotic hyphae of p. ostreatus n001 grow by tip extension with two closely spaced nuclei moving slowly forward with the growing hyphal tip. during vegetative growth of the hyphae, cytoplasmic microtubules are found as long filaments oriented longitudinally within fungal hyphae. when the apical cell reaches a length of approximately 150 μm, t ... | 2010 | 21148827 |
| evidences of high carbon catabolic enzyme activities during sporulation of pleurotus ostreatus (florida). | measurements of the specific activities of the representative enzymes of different pathways linked to carbohydrate metabolism indicate that glycolysis and tca cycles are the major route of carbohydrate catabolism in the sporulating phase of fruiting body development in pleurotus ostreatus. enzymes of the pentose phosphate pathway always showed lower specific activities as compared to those of the enzymes of the glycolytic pathway. the activity of nadp linked glutamate dehydrogenase which is know ... | 2003 | 14625896 |
| three genes specifically expressed during phosphate deficiency in pholiota nameko strain n2 encode hydrophobins. | we previously isolated 31 cdnas corresponding to pdi [inorganic phosphate (pi) deficiency- inducible] genes through the differential screening of a cdna library constructed from the mycelium of pholiota nameko strain n2 cultured in pi-depleted medium. among the cdnas, pdi251, pdi263 and pdi315 were analyzed here. the deduced amino acid sequences of pdi251, pdi263 and pdi315 showed high similarity to fungal hydrophobins and genes corresponding to these cdnas encoding p. nameko hydrophobins were d ... | 2004 | 14608471 |
| the white-rot fungus pleurotus ostreatus secretes laccase isozymes with different substrate specificities. | four laccase isozymes (lcc1, lcc2, lcc3 and lcc4) synthesized by pleurotus ostreatus strain v-184 were purified and characterized. lcc1 and lcc2 have molecular masses of about 60 and 65 kda and exhibited the same pi value (3.0). their n termini were sequenced, revealing the same amino acid sequence and homology with laccases from other microorganisms. laccases lcc3 and lcc4 were characterized by sds-page, estimating their molecular masses around 80 and 82 kda, respectively. by native isoelectrof ... | 2010 | 21149010 |
| yield, mushroom size and time to production of pleurotus cornucopiae (oyster mushroom) grown on switch grass substrate spawned and supplemented at various rates. | to find a cost effective alternative substrate, pleurotus cornucopiae 608 (yellow basidiomata) was grown on: (1) chopped, pasteurized switch grass (panicum virgatum, 99%) with 1% ground limestone and (2) a mixture of pasteurized cottonseed hulls (75% dry wt.), 24% chopped wheat straw, and 1% ground limestone (all ingredients wt./wt.). the substrates were spawned at various levels (2.5%, 3.75% or 5% wet wt., crop i) and non-supplemented or supplemented with commercial delayed release nutrient (ca ... | 2004 | 14585625 |
| purification and characterization of an aflatoxin degradation enzyme from pleurotus ostreatus. | nineteen fungi were tested for their ability to degrade aflatoxin b1 (afb1). an extracellular enzyme from the edible mushroom pleurotus ostreatus showed afaltoxin-degradation activity detected by thin-layer chromatography (tlc). an enzyme with this activity was purified by two chromatographies on deae-sepharose and phenyl-sepharose. the apparent molecular mass of the purified enzyme was estimated to be 90 kda by sds-page. optimum activities were found in the ph range between 4.0 and 5.0 and at 2 ... | 2003 | 14521233 |
| effect of tributylphenyltetraethoxylate on enzyme production of pleurotus ostreatus. | the effect of the addition of the nonionic surfactant tributylphenyltetraethoxylate to culture media on ph and extracellular protein content, and on production of beta-glucosidase, xylanase, laccase, and manganese-dependent and -independent peroxidases by the edible fungus pleurotus ostreatus was determined. the relationship between fermentation parameters and concentration of surfactant was assessed by multiple linear regression analysis, and the similarities and differences among the fermentat ... | 2003 | 12909730 |
| metal-responsive elements in pleurotus ostreatus laccase gene promoters. | fungal laccase gene transcription is strongly induced by copper ions; notably, some laccase promoters contain multiple putative metal-responsive elements (mres). previously, it has been demonstrated that the pleurotus ostreatus laccase genes poxc and poxa1b are transcriptionally induced by copper, and several putative mres were found in the promoter regions of these genes, which extend for about 400 nt upstream of the start codon (atg). identification of mre sequences, which are protected by pro ... | 2003 | 12904555 |
| a simple procedure for preparing substrate for pleurotus ostreatus cultivation. | the use of wooden crates for composting a mixture of 70% grass, (digitaria decumbens), and 30% coffee pulp, combined with 2% ca(oh)(2), was studied as a method for preparing substrate for the cultivation of pleurotus ostreatus. crate composting considerably modified the temperature pattern of the substrate in process, as compared to pile composting, where lower temperatures and less homogeneous distributions were observed. biological efficiencies varied between 59.79% and 93% in the two harvests ... | 2003 | 12895557 |
| removal of biocide pentachlorophenol in water system by the spent mushroom compost of pleurotus pulmonarius. | pentachlorophenol (pcp) has been widely used as a wood preservative since 1980s. although it has been banned worldwide, residues of pcp are still commonly found. the spent compost of oyster mushroom pleurotus pulmonarius (smc) which was a degraded paddy straw-based substrate, contained 25% chitin. five percentage of the smc could remove 89.0 +/- 0.4% of 100 mg pcpl(-1) within 2 days at room temperature predominantly by biodegradation. the maximum removal capacity was 15.5 +/- 1.0 mg g(-1) smc. t ... | 2003 | 12867185 |
| presence of pleurotus ostreatus in patagonia, argentina. | specimens belonging to the genus pleurotus were collected growing on fallen trunks of araucaria araucana, a native tree with a poorly known mycoflora, which grows in patagonia, argentina. fruitbodies were produced in culture on sawdust from an isolated strain. interspecific pairing tests performed between mating types of pleurotus from patagonia and tester strains of p. pulmonarius and p. ostreatus showed the patagonia strain to be 100% compatible with p. ostreatus and incompatible with p. pulmo ... | 2002 | 12828514 |
| mapping of genomic regions (quantitative trait loci) controlling production and quality in industrial cultures of the edible basidiomycete pleurotus ostreatus. | industrial production of the edible basidiomycete pleurotus ostreatus (oyster mushroom) is based on a solid fermentation process in which a limited number of selected strains are used. optimization of industrial mushroom production depends on improving the culture process and breeding new strains with higher yields and productivities. traditionally, fungal breeding has been carried out by an empirical trial and error process. in this study, we used a different approach by mapping quantitative tr ... | 2003 | 12788770 |
| biomass and exopolysaccharide production in submerged cultures of pleurotus ostreatoroseus sing. and pleurotus ostreatus "florida" (jack.: fr.) kummer. | the species of the genus pleurotus produce large amounts of biomass and exopolysaccharide (eps) in submerged cultures, which may be used for biotechnological purposes. in the present work two brazilian autochthonous strains of edible pleurotus (p. ostratoroseus sing. and p. ostreatus "florida") were used. the fungi grown in liquid potato dextrose medium (pd) were used as inocula to cultivate those microorganisms in the pol culture medium. after a 9-day incubation, the optimal growth time for bio ... | 2003 | 12761774 |
| the presence of partially 3-o-methylated mannogalactan from the fruit bodies of edible basidiomycetes pleurotus ostreatus 'florida' berk. and pleurotus ostreatoroseus sing. | the partially 3-o-methylated mannogalactans were isolated from the fruiting bodies of edible basidiomycetes pleurotus ostreatus 'florida' berk. and pleurotus ostreatoroseus sing. they were obtained via successive aqueous extraction, freeze thawing, and precipitation with fehling solution and then investigated using (13)c- and (1)h-nuclear magnetic resonance spectroscopy (including cosy, tocsy and hmqc techniques), methylation analysis and smith degradation. the main chain consisted of (1-->6)-li ... | 2003 | 12694919 |
| bioaugmentation of tar-contaminated soils under field conditions using pleurotus ostreatus refuse from commercial mushroom production. | the influence of the white rot fungus pleurotus ostreatus on the degradation of selected poly- and heterocyclic aromatic hydrocarbons (referred to as polycyclic aromatic hydrocarbons [pahs]) in soil was investigated under field conditions representing the northern temperate zone. pleurotus ostreatus was added to two contaminated soils in the form of homogenized refuse from the commercial production of fungus. the soils were collected from a former shipyard (the b&w soil) and underneath a former ... | 2003 | 12685699 |
| lignocellulose degradation by pleurotus ostreatus in the presence of cadmium. | activities of cellulolytic and hemicellulolytic enzymes endo-1,4-beta-glucanase, exo-1,4-beta-glucanase, 1,4-beta-glucosidase, endo-1,4-beta-xylanase, 1,4-beta-xylosidase and 1,4-beta-mannosidase and ligninolytic enzymes mn-peroxidase and laccase were detected during the growth of the white-rot fungus pleurotus ostreatus on wheat straw in the presence and absence of cadmium. the loss of substrate dry weight and mn-peroxidase activity decreased with increasing cd concentration, whereas the activi ... | 2003 | 12670686 |
| decolorization of synthetic dyes by pleurotus ostreatus isolates differing in ligninolytic properties. | the ability to decolorize four synthetic dyes (phenol red, evans blue, eosin yellowish and poly b411) in five pleurotus ostreatus strains (a parental strain and four isolates derived from it) was determined. two of the isolates had markedly higher and other two substantially lower production of ligninolytic enzymes and hydrogen peroxide than the parental strain. like the parental strain, the higher-producing isolates were able to decolorize all the tested dyes, but not to a higher extent than th ... | 2002 | 12630321 |
| production and purification of statins from pleurotus ostreatus (basidiomycetes) strains. | pleurotus ostreatus strains were cultured in liquid medium and on wheat straw. the yields of lovastatin were compared. | 2013 | 12622228 |
| infra-red spectroscopic analyses of banana waste degraded by oyster mushroom. | carbon, hydrogen and nitrogen analyses of banana leaf and pseudostem biomass revealed their potentiality as substrates for microorganisms. infra-red (ir) spectra of both biomass show presence of cellulose, xylan and lignin. ir spectra of leaf and pseudostem biomass degraded in solid state fermentation (ssf) by two pleurotus species (p. sajor-caju and p. ostreatus) for 40 days showed the utilization of cellulose, xylan and lignin by these microbes. dynamics of various lignocellulolytic enzymes of ... | 2002 | 12587734 |
| removal of phenolics in olive mill wastewaters using the white-rot fungus pleurotus ostreatus. | olive mill wastewaters (omw) present a major environmental problem. the large amounts generated, combined with the high phenols and chemical oxygen demand concentrations, are the main difficulties in finding a solution for the management of these wastewaters, which are dangerous for the environment. the phenols, which are contained in the omw have a structure similar to lignin, which makes them difficult to biodegrade. lignin can be degraded only by a few microorganisms, such as "white-rot" basi ... | 2002 | 12448515 |
| variability of laccase activity in the white-rot basidiomycete pleurotus ostreatus. | the production of laccase in liquid cultures of the white-rot fungus pleurotus ostreatus was highly variable. during the first days of cultivation, the relative variability was as high as 80-100% and it decreased to 30% in the course of cultivation. the main source of variability was assumed to be the independent development of enzyme activity in individual cultures. cultures with high laccase production showed also high production of the other ligninolytic enzyme--mn-dependent peroxidase. the v ... | 2002 | 12422515 |
| comparison of volatile compound production in fruit body and in mycelium of pleurotus ostreatus identified by submerged and solid-state cultures. | comparative analyses of the production of volatile compounds by pleurotus ostreatus jmo.95 fruit body and its corresponding mycelium grown in liquid, on agar surface, and on solid support cultures were carried out by dynamic headspace concentration using gas chromatography/mass spectrometry and gas chromatography sniffing. the aroma produced by fruit body was owing essentially to the presence of octan-3-one and, to a lesser extent, to octan-3-ol. other compounds, such as oct-1-en-3-ol, oct-1-en, ... | 2016 | 12396146 |
| mn2+ alters peroxidase profiles and lignin degradation by the white-rot fungus pleurotus ostreatus under different nutritional and growth conditions. | the white-rot fungus pleurotus ostreatus produces two types of extracellular peroxidases: manganese-dependent peroxidase (mnp) and versatile peroxidase (vp). the effect of mn2+ on fungal growth, peroxidase activity profiles, and lignin degradation by p. ostreatus was studied in liquid culture and under solid-state fermentation conditions on perlite, the latter resembling the natural growth conditions of this fungus. the fungus was grown in either a defined asparagine-containing basidiomycete sel ... | 2013 | 12396142 |
| growth and productivity of different pleurotus ostreatus strains on sunflower seed hulls supplemented with n-nh4+ and/or mn(ii). | the mycelial growth rates in lineal growth assay, yield, and production rate of five pleurotus ostreatus strains were evaluated in response to different levels of mn(ii) and/or nh4+ in a substrate containing sunflower seed hulls as a main energy and nutritional component. each strain showed different basal values for mycelial growth rate and biological efficiency on sunflower seed-hull substrate. adding growth limiting mineral nutrients increased the mycelial growth rate by 13-25%. primordia ini ... | 2002 | 12139334 |
| transformation of the edible mushroom pleurotus ostreatus by particle bombardment. | uracil auxotroph of pleurotus ostreatus was transformed to prototrophy by means of particle bombardment. five transformants were obtained under three conditions differing in the two parameters of target distance and helium pressure. the transformation frequency was one transformant per microg of dna. in the transformants, plasmid dnas were integrated into the genomic dna and stably maintained. this is the first report on transformation of p. ostreatus by particle bombardment. | 2002 | 12076804 |
| lignocellulose affects mn2+ regulation of peroxidase transcript levels in solid-state cultures of pleurotus ostreatus. | the effect of mn2+ amendment on peroxidase gene expression was studied during pleurotus ostreatus growth on cotton stalks. four peroxidase-encoding genes were expressed differentially and in a manner different from that observed in defined media. mn2+ affects mnp3 expression even 2 h after its addition to the cultures, suggesting a direct effect of the metal ion on expression. | 2002 | 12039783 |
| liquid chromatographic and electrophoretic characterisation of extracellular beta-glucosidase of pleurotus ostreatus grown in organic waste. | the production of beta-glucosidase by the ligninolytic fungus pleurotus ostreatus has been studied in different culture media containing agro-industrial wastes. the enzyme is purified by anion-exchange chromatography, the molecular mass and isoelectric point of purified beta-glucosidase are measured by sodium dodecylsulfate-polyacrylamide gel electrophoresis (sds-page) and isoelectric focusing and the stability and kinetic parameters of the enzyme assessed by spectrophotometry. it has been estab ... | 2002 | 12013217 |
| influence of spawn rate and commercial delayed release nutrient levels on pleurotus cornucopiae (oyster mushroom) yield, size, and time to production. | pleurotus cornucopiae 608 was grown on a mixture of pasteurized cottonseed hulls (75% dry wt). 24% chopped wheat straw, and 1% ground limestone. the substrate was spawned at various levels (1.25%, 2.5%, 3.75%, or 5% wet wt) and not supplemented or supplemented with commercial delayed release nutrient (campbell's s-41) at various levels (0%, 3%, 6%, 9%, or 12%). maximum yield (weight of fresh mushrooms harvested at maturity) was obtained at 3.75-5% spawn level and 6% s-41 supplement. as supplemen ... | 2002 | 11954801 |
| quantitative trait loci controlling vegetative growth rate in the edible basidiomycete pleurotus ostreatus. | mycelium growth rate is a quantitative characteristic that exhibits continuous variation. this trait has applied interest, as growth rate is correlated with production yield and increased advantage against competitors. in this work, we studied growth rate variation in the edible basidiomycete pleurotus ostreatus growing as monokaryotic or dikaryotic mycelium on eger medium or on wheat straw. our analysis resulted in identification of several genomic regions (quantitative trait loci [qtls]) invol ... | 2002 | 11872457 |
| copper and cadmium increase laccase activity in pleurotus ostreatus. | addition of copper (0.5-5 mm) or cadmium (1-5 mm) to the white rot fungus pleurotus ostreatus cultivated in liquid nitrogen-limited medium for 12 days increased the activity of laccase. the addition of 2 mm cd led to an 18.5-fold increase of activity, 1 mm cu increased the activity eight-fold. when added earlier than 12 days, the activation of laccase was delayed (cu) or decreased (cd). ag, hg, pb, zn, and h(2)o(2) decreased laccase activity. to study the effect on native enzymes, purified lacca ... | 2002 | 11786259 |
| using spectrophotometry and spectral mapping technique for the study of the production of manganese-dependent and manganese-independent peroxidases by pleurotus ostreatus. | the activity of manganese-dependent and manganese-independent peroxidases produced by pleurotus ostreatus in culture media composed of agro-residues was measured by visible spectrophotometry. the overall enzyme activity and its selectivity were separated by using spectral mapping technique followed by nonlinear mapping. the relationships between the parameters of enzyme production and the composition of culture media and fermentation time was assessed by stepwise regression analysis. calculation ... | 2002 | 11741699 |
| production of manganese peroxidase by pellet culture of the lignin-degrading basidiomycete, pleurotus ostreatus. | pleurotus ostreatus no. 42 produced the ligninolytic enzymes, manganese peroxidase (mnp) and laccase, in agitation culture in glucose/peptone/wheat-bran medium. formation of mycelial pellets 1-2 mm in diameter was essential for the production of mnp; and the concentration of dissolved oxygen in the culture medium greatly influenced the production of mnp, a concentration over 5 ppm being necessary for mnp production. the maximal activity of mnp was obtained on days 7-9 of culture, after the consu ... | 2001 | 11525618 |
| screening of pleurotus ostreatus isolates for their ligninolytic properties during cultivation on natural substrates. | thirteen basidiospore-derived isolates of pleurotus ostreatus f6 strain differing in the level of ligninolytic enzyme production and other characteristics (mycelium extension rate, colony morphology) from the parental strain were cultivated on natural substrates. under these conditions ligninolytic enzyme activity, loss of organic mass, polycyclic aromatic hydrocarbons (pahs) degradation and colonization of sterile and nonsterile soil were studied. the activity of ligninolytic enzymes was substa ... | 2000 | 11487057 |
| relationship between monokaryotic growth rate and mating type in the edible basidiomycete pleurotus ostreatus. | the edible fungus pleurotus ostreatus (oyster mushroom) is an industrially produced heterothallic homobasidiomycete whose mating is controlled by a bifactorial tetrapolar genetic system. two mating loci (mata and matb) control different steps of hyphal fusion, nuclear migration, and nuclear sorting during the onset and progress of the dikaryotic growth. previous studies have shown that the segregation of the alleles present at the matb locus differs from that expected for a single locus because ... | 2001 | 11472908 |
| transcript and activity levels of different pleurotus ostreatus peroxidases are differentially affected by mn2+. | the white-rot fungus pleurotus ostreatus produces both manganese-dependent peroxidase (mnp) and versatile peroxidase (vp) in non-manganese-amended peptone medium (pm). we studied the effect of mn2+ supplementation on mnps and vps in p. ostreatus by analysing the enzymatic and transcript abundance profiles of the peroxidases, as well as the lignin mineralization rate. the fungus was grown in pm under solid-state conditions using perlite as an inert solid support. mn2+ amendment resulted in a 1.7- ... | 2001 | 11422318 |
| homologous expression of recombinant manganese peroxidase genes in ligninolytic fungus pleurotus ostreatus. | pleurotus ostreatus is a white-rot fungus known as an efficient degrader of lignin and also various organo-pollutants. using a dna-mediated transformation system, a molecular breeding approach to isolate over-producers of a manganese peroxidase isozyme, mnp3, was carried out. recombinant mnp3 constructs under the control of p. ostreatus sdi1 expression signals were introduced into the wild-type p. ostreatus strain by co-transformation with a carboxin-resistant vector plasmid, ptm1. one of the re ... | 2001 | 11414322 |
| degradation of lignin in wheat straw during growth of the oyster mushroom (pleurotus ostreatus) using off-line thermochemolysis with tetramethylammonium hydroxide and solid-state (13)c nmr. | the oyster mushroom (pleurotus ostreatus) is widely cultivated on wheat straw (triticum aestivum); however, there is a need to better understand the relationship between the chemical composition of the compost and mushroom growth. wheat straw was degraded over a period of 63 days by p. ostreatus during which time it was sampled at weekly intervals. off-line thermochemolysis with tetramethylammonium hydroxide and solid-state (13)c nmr were then used in the molecular characterization of the undegr ... | 2001 | 11409955 |
| purification, characterization, and functional role of a novel extracellular protease from pleurotus ostreatus. | a new extracellular protease (posl; pleurotus ostreatus subtilisin-like protease) from p. ostreatus culture broth has been purified and characterized. posl is a monomeric glycoprotein with a molecular mass of 75 kda, a pi of 4.5, and an optimum ph in the alkaline range. the inhibitory profile indicates that posl is a serine protease. the n-terminal and three tryptic peptide sequences of posl have been determined. the homology of one internal peptide with conserved sequence around the asp residue ... | 2001 | 11375191 |
| [effect of culture medium composition on peroxidase biosynthesis by the fungus pleurotus ostreatus uzbi-i105]. | production of extracellular peroxidase during submerged cultivation of the xylotrophic bazidiomycete pleurotus ostreatus uzbi-i105 in nutrient media with lignocellulosic wastes, exhausted cottonseed oil cake, cotton stalks, rice husks, or ambary hemp was studied. the enzyme production increased threefold to fivefold in the presence of exhausted cottonseed oil cake extract in the nutrient medium. the dynamics of peroxidase production in various media was investigated. | 2006 | 11357429 |
| two-stage biohumus production from inedible potato biomass. | the feasibility of a two-stage bioconversion of inedible potato biomass into biohumus by oyster mushroom followed by worms was tested. as a raw material for biohumus production the inedible potato biomass in certain properties ranked below wheat straw. the most feasible method to convert the potato wastes into biohumus was to mix them with wheat straw at the mass ratio of 1:3 and then treat with mushrooms followed by worms. this gave a good yield of mushrooms. the biohumus produced from the mixt ... | 2001 | 11341687 |
| copper sorption by native and modified pellets of wood-rotting basidiomycetes. | the aim of this study was to investigate the biosorption of copper to the pellets of different wood-rotting fungal species. | 2001 | 11264752 |
| comparison of three fungal laccases from rigidoporus lignosus and pleurotus ostreatus: correlation between conformation changes and catalytic activity. | the conformation changes in solution of three fungal laccases in different environmental conditions were examined by circular dichroism (cd) and electron paramagnetic resonance (epr) spectroscopy. cd measurements indicate that the secondary structure of proteins depends slightly on the ph or ionic strength, though the presence of salt could interfere in the molecular recognition process between substrates and enzymes. the enzymes, however, are highly destabilized by prolonged exposure to low ph ... | 2001 | 11192701 |
| veratryl alcohol stimulates fruiting body formation in the oyster mushroom, pleurotus ostreatus. | the oyster mushroom, pleurotus ostreatus, cultivated in solid state on sugarcane bagasse-wheat bran (5:1) medium in the presence of veratryl alcohol resulted in an increased production of the fruiting body at earlier times compared to when the fungus was grown in the absence of veratryl alcohol. the results indicate a new physiological role for veratryl alcohol in stimulating fruiting body formation. veratryl alcohol also stimulated laccase production during the mycelial growth stage. evidence i ... | 2001 | 11164314 |
| molecular tools for breeding basidiomycetes. | the industrial production of edible basidiomycetes is increasing every year as a response to the increasing public demand of them because of their nutritional properties. about a dozen of fungal species can be currently produced for food with sound industrial and economic bases. notwithstanding, this production is threatened by biotic and abiotic factors that make it necessary to improve the fungal strains currently used in industry. breeding of edible basidiomycetes, however, has been mainly em ... | 2000 | 11032306 |
| effect of culture conditions of pleurotus ostreatus (fr.) kumm. on cellulases complexes activity in post-cultivated substrates. | the aim of the presented research was to settle conditions and parameters of pleurotus ostreatus (fr.) kumm. culture and to determine activity and yield of cellulase complexes biosynthesised by this mushroom on different substrates. wheat straw sterilized by evaporation at temperature 58-60 degrees c during 48 hours was the first substrate. wheat straw with reynoutria japonica (houtt) in 1:1 proportion, sterilized by irradiation (15 kgy), was the second, two-component, substrate. according to th ... | 2000 | 11093677 |
| degradation of bisphenol a by the lignin-degrading enzyme, manganese peroxidase, produced by the white-rot basidiomycete, pleurotus ostreatus. | degradation of 2,2-bis(4-hydroxyphenyl)propane (bisphenol a, bpa), an endocrine-disturbing chemical, by the growing mycelia of the white-rot basidiomycete, pleurotus ostreatus, was examined. about 80% of bpa initially present decreased in 12 days of culture with this fungus. by in vitro experiments using the lignin-degrading enzyme manganese peroxidase (mnp), bpa was metabolized to phenol, 4-isopropenylphenol, 4-isopropylphenol, and hexestrol. the degradation products of bpa were assumed to be f ... | 2000 | 11055402 |
| allergic asthma to shiitake and oyster mushroom. | | 2000 | 11030382 |
| characterization and immobilization of the laccase from pleurotus ostreatus and its use for the continuous elimination of phenolic pollutants. | a laccase, the only ligninolytic enzyme produced by the basidiomycete pleurotus ostreatus strain rk 36 was purified to homogeneity and characterized. the enzyme is a monomeric protein with a molecular weight of 67 000 da and an isoelectric point of 3.6. type i and type iii cu(2+) centers were identified by spectrophotometry. with syringaldazine as substrate laccase showed the highest oxidation rates at ph 5.8, 50 degrees c, and in 40 mm phosphate buffer. among the tested stabilization parameters ... | 2000 | 10899561 |
| influence of cadmium and mercury on activities of ligninolytic enzymes and degradation of polycyclic aromatic hydrocarbons by pleurotus ostreatus in soil. | the white-rot fungus pleurotus ostreatus was able to degrade the polycyclic aromatic hydrocarbons (pahs) benzo[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, dibenzo[a,h]anthracene, and benzo[ghi]perylene in nonsterile soil both in the presence and in the absence of cadmium and mercury. during 15 weeks of incubation, recovery of individual compounds was 16 to 69% in soil without additional metal. while soil microflora contributed mostly to degradation of pyre ... | 2000 | 10831426 |
| carboxin resistance transformation of the homobasidiomycete fungus pleurotus ostreatus. | a novel selection marker gene for transformation of the white-rot basidiomycete pleurotus ostreatus was developed by introducing a point mutation in a gene which encodes the iron-sulfur protein (ip) subunit of succinate dehydrogenase. the mutant gene, cbxr, encodes a modified ip subunit with an amino-acid substitution (his239 to leu) and confers resistance to the systemic fungicide, carboxin. the dna sequence was integrated ectopically in the chromosome of the transformants. this is the first re ... | 2000 | 10794179 |
| manganese peroxidase isoenzymes produced by pleurotus ostreatus grown on wood sawdust. | the white rot basidiomycete pleurotus ostreatus produces two manganese peroxidase (mnp) isoenzymes when grown in solid stationary conditions on poplar sawdust, whereas a lower production of these same enzymes is observed on fir sawdust. addition of mn(2+) to poplar culture resulted in a threefold increase of mnp activity; the same addition to fir culture was able to increase tenfold the mnp production. the two mnp isoenzymes (mnp2 and mnp3) were purified from p. ostreatus poplar culture. the iso ... | 2000 | 10729203 |