| efficient succinic acid production from lignocellulosic biomass by simultaneous utilization of glucose and xylose in engineered escherichia coli. | to enhance succinic acid formation during xylose fermentation in escherichia coli, overexpression of atp-forming phosphoenolpyruvate carboxykinase (pepck) from bacillus subtilis 168 in an ldha, pflb, and ppc deletion strain resulted in a significant increase in cell mass and succinic acid production. however, ba204 displays a low yield of glucose fermentation and sequential glucose-xylose utilization under regulation by the phosphotransferase system (pts). to improve the capability of glucose fe ... | 2013 | 24096277 |
| purification and kinetics study of thermostable cellulase free xylanase from bacillus subtilis. | present study dealt with the production, purification and characterization of xylanase from bacillus subtilis- bs05 grown in submerged fermentation at 37(o)c for 48h using sugarcane bagasse as a substrate. xylanase enzyme was purified to homogeneity by ammonium sulphate fractionation followed by sephadex g-100 chromatography. the molecular mass of xylanase enzyme was found to be 23kda by sds-page. the optimum ph and temperature of xylanase enzyme was 5 and 50°c with stability range of 5-6 and 30 ... | 2013 | 23713898 |
| high production of cellulose degrading endo-1,4-β-d-glucanase using bagasse as a substrate from bacillus subtilis kibge has. | sugarcane bagasse is a cheap carbon source for endo-1,4-β-d-glucanase production as it is easily available as by-product from sugar industries. fermentation conditions for endo-1,4-β-d-glucanase production by bacillus subtilis kibge has were optimized by using un-treated sugarcane bagasse for induction of endo-1,4-β-d-glucanase and it was found that 2.0 g% bagasse in fermentation medium induced maximum endo-1,4-β-d-glucanase production. it was also found that when sugarcane bagasse was supplemen ... | 2013 | 23044136 |
| a designed bifunctional laccase/β-1,3-1,4-glucanase enzyme shows synergistic sugar release from milled sugarcane bagasse. | a bifunctional enzyme has been created by fusing two bacillus subtilis enzymes: the β-1,3-1,4-glucanase (bgls, ec 3.2.1.73) that hydrolyzes plant cell wall β-glucans and the copper-dependent oxidase laccase (cota, ec 1.10.3.2) that catalyzes the oxidation of aromatic compounds with simultaneous reduction of oxygen to water. the chimeric laccase/β-1,3-1,4-glucanase was created by insertion fusion of the bgls and cota genes, and expressed in escherichia coli. the affinity-purified recombinant chim ... | 2013 | 23012443 |
| succinate production by metabolically engineered escherichia coli using sugarcane bagasse hydrolysate as the carbon source. | efficient biosynthesis of succinate from a renewable biomass resource by engineered escherichia coli is reported in this paper. fermentation of sugarcane bagasse hydrolysate by engineered e. coli ba204, a pflb, ldha, and ppc deletion strain overexpressing the atp-forming phosphoenolpyruvate carboxykinase from bacillus subtilis 168, produced a final succinate concentration of 15.85 g l(-1) with a high yield of 0.89 g l(-1) total sugar under anaerobic conditions. during dual-phase fermentations, i ... | 2013 | 23010211 |
| beneficial effects of bacillus subtilis subsp. subtilis mori2, a honey-associated strain, on honeybee colony performance. | a bacillus spp. strain isolated from a honey sample in morillos (salta, argentina) was phylogenetically characterized as b. subtilis subsp. subtilis mori2. the strain was administered to bee colonies as a monoculture in one litre of sugarcane syrup (125 g/l) at a final concentration of 10(5) spores/ml to evaluate the bee colony performance. the treated colony was monitored, and any changes were compared with the control hives. all conditions were identical (weather, nourishment and supervision), ... | 2012 | 26781735 |
| cry1ac and vip3aa proteins from bacillus thuringiensis targeting cry toxin resistance in diatraea flavipennella and elasmopalpus lignosellus from sugarcane. | the biological potential of vip and cry proteins from bacillus is well known and widely established. thus, it is important to look for new genes showing different modes of action, selecting those with differentiated entomotoxic activity against diatraea flavipennella and elasmopalpus lignosellus, which are secondary pests of sugarcane. therefore, cry1 and vip3 proteins were expressed in escherichia coli, and their toxicities were evaluated based on bioassays using neonate larvae. of those, the m ... | 2017 | 28123906 |
| an entomopathogenic bacterium strain, bacillus thuringiensis, as a biological control agent against the red palm weevil, rhynchophorus ferrugineus (coleoptera: curculionidae). | the red palm weevil (rpw), rhynchophorus ferrugineus, is an invasive wood-boring insect that damages palms and sugarcane. bacillus thuringiensis (bt) is an entomopathogenic bacterium which has been modified into various strains and widely used in pest management. the aim of this study was to evaluate the susceptibility of rpw to the ha strain of bt. | 2016 | 27862867 |
| possibly similar genetic basis of resistance to bacillus thuringiensis cry1ab protein in 3 resistant colonies of the sugarcane borer collected from louisiana, usa. | the sugarcane borer, diatraea saccharalis (f.), is a major maize borer pest and a target of transgenic maize expressing bacillus thuringiensis (bt) proteins in south america and the mid-southern region of the united states. evolution of resistance in target pest populations is a great threat to the long-term efficacy of bt crops. in this study, we compared the genetic basis of resistance to cry1ab protein in 3 resistant colonies of sugarcane borer established from field populations in louisiana, ... | 2016 | 27860288 |
| reproductive isolation between two populations of diatraea saccharalis (f.) (lepidoptera: crambidae) from different host plant species and regions in argentina. | the sugarcane borer, diatraea saccharalis (f.), has a widespread distribution throughout the western hemisphere and is a pest of many crop plants including sugarcane, corn, sorghum and rice. the use of bacillus thuringiensis (bt) corn has been the primary tool for managing this species in corn fields. sugarcane borer control has been recently threatened by observations of susceptibility and/or resistance to certain varieties of bt corn and the protein used in many newer varieties. this has led t ... | 2016 | 27112423 |
| lepidoptera (crambidae, noctuidae, and pyralidae) injury to corn containing single and pyramided bt traits, and blended or block refuge, in the southern united states. | fall armyworm, spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae); corn earworm, helicoverpa zea boddie (lepidoptera: noctuidae); southwestern corn borer, diatraea grandiosella dyar (lepidoptera: crambidae); sugarcane borer, diatraea saccharalis f. (lepidoptera: crambidae); and lesser cornstalk borer, elasmopalpus lignosellus zeller (lepidoptera: pyralidae), are lepidopteran pests of corn, zea mays l., in the southern united states. blended refuge for transgenic plants expressing the i ... | 2015 | 26470116 |
| identification, inheritance, and fitness costs of cry2ab2 resistance in a field-derived population of sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae). | the sugarcane borer, diatraea saccharalis (f.), is one of the major target pests of transgenic maize, zea mays, expressing bacillus thuringiensis (bt) proteins in south america and mid-southern region of the u.s. the mon89034 maize expresses cry2ab2 and cry1a.105 bt proteins and it was developed to provide better control of key lepidopteran pests of maize including d. saccharalis. the objectives of this study were to select and characterize the resistance of d. saccharalis to cry2ab2 using a non ... | 2015 | 26205174 |
| binding analysis of bacillus thuringiensis cry1 proteins in the sugarcane borer, diatraea saccharalis (lepidoptera: crambidae). | sugarcane borer (diatraea saccharalis, f.) is an important corn pest in south america and united states. the aim of the present study was to analyze the susceptibility and binding interactions of three cry1a proteins and cry1fa in a brazilian d. saccharalis population. the results showed that cry1ab was the most active, followed by cry1ac, cry1fa and cry1aa. all cry1-biotinylated proteins tested bound specifically to the d. saccharalis brush border membrane vesicles (bbmv). heterologous competit ... | 2015 | 25736726 |
| modeling evolution of resistance of sugarcane borer (lepidoptera: crambidae) to transgenic bt corn. | diatraea saccharalis (f.) (lepidoptera: crambidae) is a target pest of transgenic corn expressing bacillus thuringiensis (bt) protein, and the first evidence of resistance by d. saccharalis to cry1ab corn was detected in a field population in northeast louisiana in 2004. we used a model of population dynamics and genetics of d. saccharalis to 1) study the effect of interfield dispersal, the first date that larvae enter diapause for overwintering, toxin mortality, the proportion of non-bt corn in ... | 2014 | 24914780 |
| managing the sugarcane borer, diatraea saccharalis, and corn earworm, helicoverpa zea, using bt corn and insecticide treatments. | the sugarcane borer, diatraea saccharalis (fabricius) (lepidoptera: crambidae) and the corn earworm, helicoverpa zea (boddie) (lepidoptera: noctuidae), are important pests of corn in brazil and have not been successfully managed, because of the difficulty of managing them with pesticides. the objective of this study was to evaluate the effect of bt corn mon810, transformed with a gene from bacillus thuringiensis berliner (bacillales: bacillaceae) insecticide seed treatment, and foliar insecticid ... | 2013 | 24735131 |
| control of diatraea saccharalis by the endophytic pantoea agglomerans 33.1 expressing cry1ac7. | despite the fact that bacillus thuringiensis (bt) is found in more than 90 % of the products used against insects, it has some difficulty reaching the internal regions where the larvae feed. to solve this problem, many genetically modified microorganisms that colonize the same pests have been developed. thus, the endophytic bacterium pantoea agglomerans (33.1), which has been recently described as a promising sugarcane growth promoter, was genetically modified with the pjtt vector (which carries ... | 2014 | 24531524 |
| fitness costs and stability of cry1ab resistance in sugarcane borer, diatraea saccharalis (f.). | the sugarcane borer, diatraea saccharalis (f.), is a major target species of transgenic corn expressing bacillus thuringiensis (bt) proteins in south america and the u.s. mid-south region. in this study, the fitness of seven insect genotypes of d. saccharalis were assayed on non-toxic diet, which included a cry1ab-susceptible strain (ss-2009), two cry1ab-resistant strains (rr-43a(bc), rr-l5b(bc)), and four f1 hybrids (f1-r43a(m)s(f), f1-r43a(f)s(m), f1-r5b(m)s(f), and f1-r5b(f)s(m)). the f1 hybr ... | 2014 | 24503242 |
| potential of sugarcane bagasse (agro-industrial waste) for the production of bacillus thuringiensis israelensis. | sugarcane bagasse is a renewable resource that can be used to produce biopesticide for the control of mosquito vectors. in the present study, we demonstrated that cane processed bagasse could be used to produce bacillus thuringiensis serovar israelensis (bti) for control of mosquito vectors viz: culex quinquefasciatus, anopheles stephensi and aedes aegypti. biochemical studies indicated that the bti spore/crystal toxins produced from the test culture medium (bagasse, bg + soybean, sb) are higher ... | 2013 | 24189680 |
| microbial degradation of fipronil by bacillus thuringiensis. | fipronil, a phenyl pyrazole insecticide has been found to be effective for the control of various insect pests. due to its higher persistence in soil bioremediation is a promising approach to degrade the pesticide from soil. isolation and identification of soil microbes was conducted for bioremediation of fipronil contaminated soils. soil samples collected from different sugarcane growing fields in gurdaspur district with extensive use of pesticide history served as a source of pesticide degradi ... | 2013 | 23618775 |
| occurrence and larval movement of diatraea saccharalis (lepidoptera: crambidae) in seed mixes of non-bt and bt pyramid corn. | larval movement of target pest populations among bt and non-bt plants is a major concern in the use of a seed mixture refuge strategy for bt resistance management. in this study, occurrence and larval movement of the sugarcane borer, diatraea saccharalis (f.), were evaluated in four planting patterns of non-bt and bt plants containing genuity® smartstax(tm) traits in 2009-2011. the four planting patterns were: (1) a pure stand of 27 bt plants; (2) one non-bt plant in the center, surrounded by 26 ... | 2013 | 23456950 |
| evaluation of corn hybrids expressing cry1f, cry1a.105, cry2ab2, cry34ab1/cry35ab1, and cry3bb1 against southern united states insect pests. | studies were conducted across the southern united states to characterize the efficacy of multiple bacillus thuringiensis (bt) events in a field corn, zea mays l., hybrid for control of common lepidopteran and coleopteran pests. cry1f protein in event tc1507 and cry1a.105 + cry2ab2 proteins in event mon 89034 were evaluated against pests infesting corn on above-ground plant tissue including foliage, stalks, and ears. cry34ab1/cry35ab1 proteins in event das-59122-7 and cry3bb1 in event mon 88017 w ... | 2012 | 23156183 |
| extended monitoring of resistance to bacillus thuringiensis cry1ab maize in diatraea saccharalis (lepidoptera: crambidae). | the sugarcane borer, diatraea saccharalis (f.), is a major target of transgenic maize expressing bacillus thuringiensis (bt) proteins in south america and the mid-southern region of the united states. during 2007-2009, a total of 986 feral individuals of d. saccharalis were collected from maize fields in six locations of louisiana and mississippi and examined for resistance to cry1ab maize using f 1/f 2 screens. major resistance alleles to cry1ab maize in the populations sampled from non-bt maiz ... | 2014 | 22688686 |
| parasitism by the endoparasitoid, cotesia flavipes induces cellular immunosuppression and enhances susceptibility of the sugar cane borer, diatraea saccharalis to bacillus thuringiensis. | cotesia flavipes cameron (hymenoptera: braconidae), is a gregarious larval endoparasitoid of the sugarcane borer, diatraea saccharalis fabricius (lepidoptera: crambidae). the aim of this research was to analyze cellular immunosuppression of d. saccharalis parasitized by c. flavipes in terms of encapsulation, melanization, and hemocyte nodule formation. the encapsulation assay was done 1 and 6 days after parasitoid oviposition. in addition, the susceptibility of parasitized and nonparasitzed larv ... | 2011 | 22225507 |
| isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from bacillus vallismortis rg-07. | the rising concerns about the scarcity of fossil fuels, the emission of green house gasses and air pollution by incomplete combustion of fossil fuel have also resulted in an increasing focus on the use of cellulases to perform enzymatic hydrolysis of the lignocellulosic materials for the generation of bioethanol. the aim of this study was to isolate a potential thermo-solvent tolerant cellulase producing bacterium from natural resources, and then applied for purification and characterization. th ... | 2015 | 25886936 |
| selection of reference genes for gene expression studies in virus-infected monocots using quantitative real-time pcr. | both genome-wide transcriptomic surveys of the mrna expression profiles and virus-induced gene silencing-based molecular studies of target gene during virus-plant interaction involve the precise estimation of the transcript abundance. quantitative real-time pcr (qpcr) is the most widely adopted technique for mrna quantification. in order to obtain reliable quantification of transcripts, identification of the best reference genes forms the basis of the preliminary work. nevertheless, the stabilit ... | 2013 | 23954326 |
| enhanced transgene expression in sugarcane by co-expression of virus-encoded rna silencing suppressors. | post-transcriptional gene silencing is commonly observed in polyploid species and often poses a major limitation to plant improvement via biotechnology. five plant viral suppressors of rna silencing were evaluated for their ability to counteract gene silencing and enhance the expression of the enhanced yellow fluorescent protein (eyfp) or the β-glucuronidase (gus) reporter gene in sugarcane, a major sugar and biomass producing polyploid. functionality of these suppressors was first verified in n ... | 2013 | 23799071 |
| beauveria brongniartii on white grubs attacking sugarcane in south africa. | beauveria brongniartii (saccardo) petch fungal infections were observed on the melolonthid hypopholis sommeri burmeister (coleoptera: scarabaeidae) at two sites (harden heights and canema) in the sugarcane producing area of the northern kwazulu-natal midlands of south africa. to initially identify the disease-causing fungus, 17 different fluorescently-labelled microsatellite pcr primers were used to target 78 isolates of beauveria spp. dna. microsatellite data resolved two distinct clusters of b ... | 2012 | 22982079 |
| description and phylogenetic placement of beauveria hoplocheli sp. nov. used in the biological control of the sugarcane white grub, hoplochelus marginalis, on reunion island. | on reunion island successful biological control of the sugarcane white grub hoplochelus marginalis fairmaire (coleoptera: melolonthidae) has been conducted for decades with strains from the entomopathogenic fungal genus beauveria (ascomycota: hypocreales). a study based on morphological characters combined with a multisequence phylogenetic analysis of genes that encode the translation elongation factor 1-alpha (tef1), rna polymerase ii largest subunit (rpb1), rna polymerase ii second largest sub ... | 2016 | 26297783 |
| experimental investigation of microbiologically influenced corrosion of selected steels in sugarcane juice environment. | in the current study, ferritic stainless grades aisi 439 and aisi 444 were investigated as possible construction materials for machinery and equipment in the cane-sugar industry. their performance in corrosive cane-sugar juice environment was compared with the presently used low carbon steel aisi 1010 and austenitic stainless steel aisi 304. the tafel plot electrochemical technique was used to evaluate general corrosion performance. microbiologically influenced corrosion (mic) behaviour in sugar ... | 2013 | 23764955 |
| maize elongin c interacts with the viral genome-linked protein, vpg, of sugarcane mosaic virus and facilitates virus infection. | the viral genome-linked protein, vpg, of potyviruses is involved in viral genome replication and translation. to determine host proteins that interact with sugarcane mosaic virus (scmv) vpg, a yeast two-hybrid screen was used and a maize (zea mays) elongin c (zmelc) protein was identified. zmelc transcript was observed in all maize organs, but most highly in leaves and pistil extracts, and zmelc was present in the cytoplasm and nucleus of maize cells in the presence or absence of scmv. zmelc exp ... | 2014 | 24954157 |
| de novo analysis of the transcriptome of pratylenchus zeae to identify transcripts for proteins required for structural integrity, sensation, locomotion and parasitism. | the root lesion nematode pratylenchus zeae, a migratory endoparasite, is an economically important pest of major crop plants (e.g. cereals, sugarcane). it enters host roots, migrates through root tissues and feeds from cortical cells, and defends itself against biotic and abiotic stresses in the soil and in host tissues. we report de novo sequencing of the p. zeae transcriptome using 454 flx, and the identification of putative transcripts encoding proteins required for movement, response to stim ... | 2016 | 26292651 |
| biodegradation of s-triazine herbicide atrazine by enterobacter cloacae and burkholderia cepacia sp. from long-term treated sugarcane-cultivated soils in kenya. | in this study soils from sugarcane-cultivated fields were screened for bacterial species capable of atrazine (6-chloro-n²-ethyl-n⁴-isopropyl-1,3,5-triazine-2,4-diamine) degradation due to long exposure of the soils to this herbicide. to enrich for atrazine degraders, minimal salt medium containing atrazine as the sole n source and glucose as the c source was inoculated with soils impacted with this herbicide and incubated. bacterial growth was monitored by measuring optical density. the degradat ... | 2012 | 22575004 |
| draft genome sequence of xanthomonas axonopodis pathovar vasculorum ncppb 900. | xanthomonas axonopodis pathovar vasculorum strain ncppb 900 was isolated from sugarcane on reunion island in 1960. consistent with its belonging to fatty-acid type d, multi-locus sequence analysis confirmed that ncppb 900 falls within the species x. axonopodis. this genome harbours sequences similar to plasmids pxcv183 from x. campestris pv. vesicatoria 85-10 and pphb194 from burkholderia pseudomallei. its repertoire of predicted effectors includes homologues of xopaa, xopad, xopae, xopb, xopd, ... | 2014 | 25263632 |
| bacilliform dna-containing plant viruses in the tropics: commonalities within a genetically diverse group. | plant viruses, possessing a bacilliform shape and containing double-stranded dna, are emerging as important pathogens in a number of agricultural and horticultural crops in the tropics. they have been reported from a large number of countries in african and asian continents, as well as from islands from the pacific region. the viruses, belonging to two genera, badnavirus and tungrovirus, within the family caulimoviridae, have genomes displaying a common plan, yet are highly variable, sometimes e ... | 2013 | 23763585 |
| recovery of purified lactonic sophorolipids by spontaneous crystallization during the fermentation of sugarcane molasses with candida albicans o-13-1. | numerous studies have focused on how to obtain high yield of sophorolipids using low-cost materials as substrates, and there has been various work on the experimental methods for purifying lactonic sophorolipids. these studies have not yet obtained satisfied results in combining a low-cost fermentation process and the purification of lactonic sophorolipids. this study establishes a fed-batch fermentation process of purifying sophorolipids from candida albicans o-13-1 using low-cost sugarcane mol ... | 2012 | 23040390 |
| diversity of culturable yeasts in phylloplane of sugarcane in thailand and their capability to produce indole-3-acetic acid. | yeasts were isolated by the enrichment technique from the phylloplane of 94 samples of sugarcane leaf collected from seven provinces in thailand. all sugarcane leaf samples contained yeasts and 158 yeast strains were obtained. on the basis of the d1/d2 domain of the large subunit rrna gene sequence analysis, 144 strains were identified to 24 known species in 14 genera belonging to the ascomycota viz. candida akabanensis, candida dendronema, candida mesorugosa, candida michaelii, candida nivarien ... | 2014 | 24442819 |
| production of mannitol from a high concentration of glucose by candida parapsilosis sk26.001. | a novel strain, sk26.001, which can produce mannitol from a high concentration of glucose without the addition of fructose, was isolated from sugarcane juice. this strain was identified as candida parapsilosis based on 18s ribosomal rna (rrna) sequence analysis and the morphological and physiological-biochemical characteristics of the strain. under optimized fermentation conditions, the mannitol concentration in shake flasks reached 68.5 g/l. when batch fermentation was performed, the fed glucos ... | 2017 | 27557902 |
| response surface methodology for optimizing the production of biosurfactant by candida tropicalis on industrial waste substrates. | biosurfactant production optimization by candida tropicalis ucp0996 was studied combining central composite rotational design (ccrd) and response surface methodology (rsm). the factors selected for optimization of the culture conditions were sugarcane molasses, corn steep liquor, waste frying oil concentrations and inoculum size. the response variables were surface tension and biosurfactant yield. all factors studied were important within the ranges investigated. the two empirical forecast model ... | 2017 | 28223971 |
| fungal communities in pressmud composting harbour beneficial and detrimental fungi for human welfare. | pressmud is a substrate derived from sugarcane juice filtrate, and around 26-40 kg of this residue are produced per ton of sugarcane. it is mainly used as fertilizer in crops, and its application in the field is often made without any prior treatment, but, in this research, it was studied for the risk this practice poses for human health. this research was stimulated by previous results indicating the presence of opportunistic pathogens in residues used in various composting systems and the ext ... | 2016 | 27170376 |
| xylitol production by yeasts isolated from rotting wood in the galápagos islands, ecuador, and description of cyberlindnera galapagoensis f.a., sp. nov. | this study evaluated d-xylose-assimilating yeasts that are associated with rotting wood from the galápagos archipelago, ecuador, for xylitol production from hemicellulose hydrolysates. a total of 140 yeast strains were isolated. yeasts related to the clades yamadazyma, kazachstania, kurtzmaniella, lodderomyces, metschnikowia and saturnispora were predominant. in culture assays using sugarcane bagasse hemicellulose hydrolysate, candida tropicalis clqca-24sc-125 showed the highest xylitol producti ... | 2015 | 26219566 |
| diversity and physiological characterization of d-xylose-fermenting yeasts isolated from the brazilian amazonian forest. | this study is the first to investigate the brazilian amazonian forest to identify new d-xylose-fermenting yeasts that might potentially be used in the production of ethanol from sugarcane bagasse hemicellulosic hydrolysates. | 2012 | 22912807 |
| decolorization of a recalcitrant organic compound (melanoidin) by a novel thermotolerant yeast, candida tropicalis rg-9. | sugarcane distilleries use molasses for ethanol production and generate large volume of effluent containing high biological oxygen demand (bod) and chemical oxygen demand (cod) along with melanoidin pigment. melanoidin is a recalcitrant compound that causes several toxic effects on living system, therefore, may be treated before disposal. the aim of this study was to isolate a potential thermotolerant melanoidin decolorizing yeast from natural resources, and optimized different physico-chemical ... | 2012 | 22708874 |
| complete genome sequence of nine isolates of canna yellow streak virus reveals its relationship to the sugarcane mosaic virus (scmv) subgroup of potyviruses. | complete genome sequences were obtained from nine isolates of canna yellow streak virus (caysv). caysv belongs to the sugarcane mosaic virus (scmv) subgroup of potyviruses with johnsongrass mosaic virus (jgmv) as its closest relative. multiple sequence alignments showed a pattern of amino acid substitutions in the cp sequences, which enabled us to relate these isolates to south east asian or european isolates. biological characterization of caysv identified nicotiana benthamiana, chenopodium qui ... | 2015 | 25567205 |
| comparison of the impact of viral and plant-derived promoters regulating selectable marker gene on maize transformation and transgene expression. | the choice of promoter regulating the selectable marker gene impacts transformation efficiency, copy number and the expression of selectable marker and flanking genes in maize. viral or plant-derived constitutive promoters are often used to regulate selectable marker genes. we compared two viral promoters, cauliflower mosaic virus (camv 35t) and sugarcane bacilliform virus (scbv) with two plant promoters, rice actin1 (osact1) and maize ubiquitin 1 (zmubi1) to drive aryloxyalkanoate dioxygenase ( ... | 2017 | 28160062 |
| truncated ubiquitin 5' regulatory region from erianthus arundinaceus drives enhanced transgene expression in heterologous systems. | characterization of novel plant gene promoters underpins the development of transgenic crop plants. here, we report a novel 5' regulatory sequence (eriubi d7) of the ubiquitin gene from erianthus arundinaceus, a wild relative of sugarcane resistant to many biotic and abiotic stresses. a 3.2-kb regulatory sequence of ubiquitin gene was isolated through random amplification of genomic ends technique and characterized in rice, tobacco, and sugarcane. in silico analysis revealed that the regulatory ... | 2015 | 25985900 |
| 5' regulatory region of ubiquitin 2 gene from porteresia coarctata makes efficient promoters for transgene expression in monocots and dicots. | porteresia ubiquitin 5' regulatory region drives transgene expression in monocots and dicots. ubiquitin promoters are promising candidates for constitutive transgene expression in plants. in this study, we isolated and characterized a novel 5' regulatory sequence of a ubiquitin gene from porteresia coarctata, a stress-tolerant wild grass species. through functional analysis in heterologous plant systems, we have demonstrated that full length (port ubi2.3) or truncated sequence (pd2) of the isola ... | 2013 | 23508257 |
| reuteran and levan as carbohydrate sinks in transgenic sugarcane. | the present study reports the effect of high molecular weight bacterial fructan (levan) and glucan (reuteran) on growth and carbohydrate partitioning in transgenic sugarcane plants. these biopolymers are products of bacterial glycosyltransferases, enzymes that catalyze the polymerization of glucose or fructose residues from sucrose. constructs, targeted to different subcellular compartments (cell wall and cytosol) and driven by the cauliflower mosaic virus-35s: maize-ubiquitin promoter, were int ... | 2012 | 22903192 |
| ima genome-f 6: draft genome sequences of armillaria fuscipes, ceratocystiopsis minuta, ceratocystis adiposa, endoconidiophora laricicola, e. polonica and penicillium freii daomc 242723. | the genomes of armillaria fuscipes, ceratocystiopsis minuta, ceratocystis adiposa, endoconidiophora laricicola, e. polonica, and penicillium freii daomc 242723 are presented in this genome announcement. these six genomes are from plant pathogens and otherwise economically important fungal species. the genome sizes range from 21 mb in the case of ceratocystiopsis minuta to 58 mb for the basidiomycete armillaria fuscipes. these genomes include the first reports of genomes for the genus endoconidio ... | 2016 | 27433447 |
| expression of two novel β-glucosidases from chaetomium atrobrunneum in trichoderma reesei and characterization of the heterologous protein products. | two novel gh3 family thermostable β-glucosidases from the filamentous fungus chaetomium atrobrunneum (cel3a and cel3b) were expressed in trichoderma reesei, purified by two-step ion exchange chromatography, and characterized. both enzymes were active over a wide range of ph as compared to neurospora crassa β-glucosidase gh3-3, which was also expressed in t. reesei and purified. the optimum temperature of both c. atrobrunneum enzymes was around 60 °c at ph 5, and both enzymes had better thermal a ... | 2016 | 27714589 |
| the genome organisation and taxonomy of sugarcane striate mosaic associated virus. | sugarcane striate mosaic associated virus (scsmav) has slightly flexuous 950 nm x 15 nm filamentous particles and is associated with sugarcane striate mosaic disease in central queensland, australia. we report the full sequence of its rna genome, which comprises 5 open reading frames representing the polymerase, movement function proteins encoded in a triple gene block and coat protein. phylogenetic analyses based on either the full nucleotide sequence, the polymerase protein, or the coat protei ... | 2001 | 11676409 |
| production and characterization of violacein by locally isolated chromobacterium violaceum grown in agricultural wastes. | the present work highlighted the production of violacein by the locally isolated chromobacterium violaceum (genbank accession no. hm132057) in various agricultural waste materials (sugarcane bagasse, solid pineapple waste, molasses, brown sugar), as an alternative to the conventional rich medium. the highest yield for pigment production (0.82 g l⁻¹) was obtained using free cells when grown in 3 g of sugarcane bagasse supplemented with 10% (v/v) of l-tryptophan. a much lower yield (0.15 g l⁻¹) wa ... | 2012 | 22278051 |
| biohydrogen production from hydrolysates of selected tropical biomass wastes with clostridium butyricum. | biohydrogen production has received widespread attention from researchers in industry and academic fields. response surface methodology (rsm) was applied to evaluate the effects of several key variables in anaerobic fermentation of glucose with clostridium butyrium, and achieved the highest production rate and yield of hydrogen. highest h2 yield of 2.02 mol h2/mol-glucose was achieved from 24 h bottle fermentation of glucose at 35 °c, while the composition of medium was (g/l): 15.66 glucose, 6.0 ... | 2016 | 27251222 |
| loop-mediated isothermal amplification (lamp) based detection of colletotrichum falcatum causing red rot in sugarcane. | red rot, caused by colletotrichum falcatum, is a destructive disease prevalent in most sugarcane-producing countries. disease-free sugarcane planting materials (setts) are essential as the pathogen spreads primarily through infected setts. the present study was undertaken to develop a loop-mediated isothermal amplification (lamp) assay for the detection of c. falcatum. c. falcatum genomic dna was isolated from pure mycelium culture and infected tissues. four sets of primers corresponding to a un ... | 2015 | 25861736 |
| a betabaculovirus encoding a gp64 homolog. | a betabaculovirus (disagv) was isolated from diatraea saccharalis (lepidoptera: crambidae), one of the most important insect pests of the sugarcane and other monocot cultures in brazil. | 2016 | 26847652 |
| isolation and identification of hexazinone-degrading bacterium from sugarcane-cultivated soil in kenya. | the s-triazine herbicide hexazinone [3-cyclohexyl-6-dimethylamino-1-methyl-1,3,5-triazine-2,4(1h,3h)-dione], is widely used in agriculture for weed control. laboratory biodegradation experiments for hexazinone in liquid cultures were carried out using sugarcane-cultivated soils in kenya. liquid culture experiments with hexazinone as the only carbon source led to the isolation of a bacterial strain capable of its degradation. through morphological, biochemical and molecular characterization by 16 ... | 2014 | 24458247 |
| biological neutralization and biosorption of dyes of alkaline textile industry wastewater. | the present work was aimed to secure biological neutralization and biosorption of dyes of an alkaline textile industrial effluent (atie) using an alkaliphilic bacterium, enterococcus faecalis strain r-16 isolated from gujarat coast. the isolate was capable and competent to bring down the ph of atie from 12.1 to 7.0 within 2 h in the presence of carbon and nitrogen sources. carboxylic group concentration (cgc), nmr and ft-ir analysis revealed production of carboxylic acid as a result of neutraliz ... | 2014 | 24910184 |
| identification and molecular characterization of a novel sugarcane streak mastrevirus and an isolate of the a-strain of maize streak virus from sugarcane in nigeria. | sugarcane and maize plants showing symptoms typical of those described for the so-called "african streak viruses" (afsvs) were encountered during field surveys conducted from february to july 2015 to document viruses infecting both crops across the northern guinea savannah region of nigeria. as part of this study, two categories of complete mastrevirus-like genome sequences were obtained from nine samples (maize = 2; sugarcane = 7). in pairwise comparisons, the full-length genomes of the first s ... | 2017 | 27815694 |
| isolation of saccharomyces cerevisiae from pineapple and orange and study of metal's effectiveness on ethanol production. | in view of the anticipated shortage of the traditional supplies of fossil fuels, there is a great deal of interest in the production of ethanol as an alternative biofuel in recent years. the main objective of this research work was to isolate and characterize stress tolerant, high potential ethanol producing yeast strains from various fruit peel. two yeast isolates from pineapple (pa) and orange (or) have been isolated, characterized on the basis of morphological and physic-chemical characters a ... | 2017 | 28386473 |
| long-term t-dna insert stability and transgene expression consistency in field propagated sugarcane. | this study addresses t-dna insert stability and transgene expression consistency in multiple cycles of field propagated sugarcane. t-dna inserts are stable; no transgene rearrangements were observed. amcyan1 and pmi protein accumulation levels were maintained. there was no evidence that production of either protein declined across generations and no transgene silencing was observed in three commercial sugarcane varieties through commercially relevant ratooning, propagation-by-setts, and micro-pr ... | 2017 | 28032251 |
| the family 22 carbohydrate-binding module of bifunctional xylanase/β-glucanase xyn10e from paenibacillus curdlanolyticus b-6 has an important role in lignocellulose degradation. | a newly isolated endo-β-1,4-xylanase (xyn10e) from paenibacillus curdlanolyticus b-6 has a modular structure consisting of a family 22 carbohydrate-binding module (cbm), a glycoside hydrolase (gh) family 10 catalytic domain, two fibronectin type iii (fn3) domains, and a family 3 cbm at the c-terminus. intact xyn10e (rxyn10e), cbm22-deleted xyn10e (x-cbm3), cbm3-deleted xyn10e (x-cbm22), and gh10 catalytic domain only (x-gh10) were expressed in escherichia coli. rxyn10e showed bifunctional degrad ... | 2017 | 27871388 |
| production of d-lactate from sugarcane bagasse and corn stover hydrolysates using metabolic engineered escherichia coli strains. | in this study, the lactogenic escherichia coli strain ju15 was used and modified to produce d-lactate (d-la) from plant hydrolysates with a minimal nutrient addition in ph controlled fermenters. results showed that strain ju15 produces d-la with high yield and productivity in laboratory simulated hydrolysate media and actual sugar cane bagasse hemicellulosic hydrolysate. strain ju15 showed sequential carbon source utilization and acetic acid production. the l-lactic and acetic acid production pa ... | 2016 | 27573474 |
| effective pretreatment of sugarcane bagasse with combination pretreatment and its hydrolyzates as reaction media for the biosynthesis of ethyl (s)-4-chloro-3-hydroxybutanoate by whole cells of e. coli cczu-k14. | in this study, sugarcane bagasse (sb) was pretreated with combination pretreatment (e.g., sequential koh extraction and ionic liquid soaking, sequential koh extraction and fenton soaking, or sequential koh extraction and glycerol soaking). after the enzymatic hydrolysis of pretreated sbs, it was found that all these three concentrated hydrolyzates could be used for the asymmetric bioreduction of ethyl 4-chloro-3-oxobutanoate (cobe) into ethyl (s)-4-chloro-3-hydroxybutanoate [(s)-chbe]. compared ... | 2016 | 27060248 |
| activated carbon from pyrolysed sugarcane bagasse: silver nanoparticle modification and ecotoxicity assessment. | activated carbon from pyrolysed sugarcane bagasse (acpb) presented pore size ranges from 1.0 to 3.5nm, and surface area between 1200 and 1400m(2)g(-1) that is higher than commonly observed to commercial activated carbon. the acpb material was successfully loaded with of silver nanoparticles with diameter around 35nm (0.81wt.%). x-ray photoelectron spectroscopy (xps) analyses showed that the material surface contains metallic/ag(0) (93.60wt.%) and ionic/ag(+) states (6.40wt.%). the adsorption cap ... | 2016 | 27039274 |
| purification and characterization of recombinant sugarcane sucrose phosphate synthase expressed in e. coli and insect sf9 cells: an importance of the n-terminal domain for an allosteric regulatory property. | sucrose phosphate synthase (sps) catalyses the transfer of glycosyl group of uridine diphosphate glucose to fructose-6-phosphate to form sucrose-6-phosphate. plant sps plays a key role in photosynthetic carbon metabolisms, which activity is modulated by an allosteric activator glucose-6-phosphate (g6p). we produced recombinant sugarcane sps using escherichia coli and sf9 insect cells to investigate its structure-function relationship. when expressed in e. coli, two forms of sps with different si ... | 2016 | 26826371 |
| plasmidic expression of nema and yafc* increased resistance of ethanologenic escherichia coli ly180 to nonvolatile side products from dilute acid treatment of sugarcane bagasse and artificial hydrolysate. | hydrolysate-resistant escherichia coli sl100 was previously isolated from ethanologenic ly180 after sequential transfers in am1 medium containing a dilute acid hydrolysate of sugarcane bagasse and was used as a source of resistance genes. many genes that affect tolerance to furfural, the most abundant inhibitor, have been described previously. to identify genes associated with inhibitors other than furfural, plasmid clones were selected in an artificial hydrolysate that had been treated with a v ... | 2016 | 26826228 |
| [succinic acid production from sucrose and sugarcane molasses by metabolically engineered escherichia coli]. | sugarcane molasses containing large amounts of sucrose is an economical substrate for succinic acid production. however, escherichia coli afp111 cannot metabolize sucrose although it is a promising candidate for succinic acid production. to achieve sucrose utilizing ability, we cloned and expressed cscbka genes encoding sucrose permease, fructokinase and invertase of non-pts sucrose-utilization system from e. coli w in e. coli afp111 to generate a recombinant strain afp111/pmd19t-cscbka. after 7 ... | 2015 | 26380410 |
| improved waste water treatment by bio-synthesized graphene sand composite. | the photocatalytic and antibacterial properties of graphene biosynthesized from sugar and anchored on sand particles has been focused here. the morphology and composition of the synthesized graphene sand composite (gsc) was investigated by means of x-ray powder diffraction (xrd), scanning electron microscopy (sem), energy dispersive x-ray spectroscopy (edax), fourier transform infra-red spectroscopy (ftir) and uv-visible spectroscopy. sem images show wrinkly edges. this is characteristic of grap ... | 2015 | 26265599 |
| improved ethanol production from biomass by a rumen metagenomic dna fragment expressed in escherichia coli ms04 during fermentation. | with the aim of improving current ethanologenic escherichia coli strains, we screened a metagenomic library from bovine ruminal fluid for cellulolytic enzymes. we isolated one fosmid, termed csd4, which was able to confer to e. coli the ability to grow on complex cellulosic material as the sole carbon source such as avicel, carboxymethyl cellulose, filter paper, pretreated sugarcane bagasse, and xylan. glucanolytic activity obtained from e. coli transformed with csd4 was maximal at 24 h of incub ... | 2015 | 26175105 |
| mutation in galp improved fermentation of mixed sugars to succinate using engineered escherichia coli as1600a and am1 mineral salts medium. | escherichia coli kj122 was engineered to produce succinate from glucose using the wild type galp for glucose uptake instead of the native phosphotransferase system (ptsi mutation). this strain now ferments 10% xylose poorly. mutants were selected by serial transfers in am1 mineral salts medium with 10% xylose. clones from this population all exhibited a similar improvement, co-fermentation of an equal mixture of xylose and glucose. one of these, as1600a, produced 84.26 ± 1.37 g/l succinate, equi ... | 2015 | 26159300 |
| overexpression and characterization of a glucose-tolerant β-glucosidase from t. aotearoense with high specific activity for cellobiose. | thermoanaerobacterium aotearoense p8g3#4 produced β-glucosidase (bgl) intracellularly when grown in liquid culture on cellobiose. the gene bgl, encoding β-glucosidase, was cloned and sequenced. analysis revealed that the bgl contained an open reading frame of 1314 bp encoding a protein of 446 amino acid residues, and the product belonged to the glycoside hydrolase family 1 with the canonical glycoside hydrolase family 1 (gh1) (β/α)8 tim barrel fold. expression of pet-bgl together with a chaperon ... | 2015 | 25957152 |
| the antibiotic activity and mechanisms of sugarcane (saccharum officinarum l.) bagasse extract against food-borne pathogens. | sugarcane bagasse contains natural compositions that can significantly inhibit food-borne pathogens growth. in the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol. the sugarcane bagasse extract showed bacteriostatic activity against the growth of staphylococcus aureus, listeria monocytogenes, escherichia coli and salomonella typhimurium. additionally, the sugarcane bagasse extract can increase the electri ... | 2015 | 25952848 |
| combining treatments to improve the fermentation of sugarcane bagasse hydrolysates by ethanologenic escherichia coli ly180. | inhibitory side products from dilute acid pretreatment is a major challenge for conversion of lignocellulose into ethanol. six strategies to detoxify sugarcane hydrolysates were investigated alone, and in combinations (vacuum evaporation of volatiles, high ph treatment with ammonia, laccase, bisulfite, microaeration, and inoculum size). high ph was the most beneficial single treatment, increasing the minimum inhibitory concentration (measured by ethanol production) from 15% (control) to 70% hydr ... | 2015 | 25864026 |
| phosphomannose isomerase affects the key enzymes of glycolysis and sucrose metabolism in transgenic sugarcane overexpressing the mana gene. | sugarcane is one of the most important crops cultivated for the production of sugar and ethanol. in our previous studies, an innovative positive selection system for obtaining transgenic sugarcane, which utilized the e. coli-derived mana gene as the selectable marker and mannose as the selective agent, was developed and patented in china. in this paper, the influence of phosphomannose isomerase (pmi) overexpression on the key enzymes of both glycolysis and sucrose metabolism was investigated in ... | 2017 | 25798049 |
| escherichia coli w shows fast, highly oxidative sucrose metabolism and low acetate formation. | sugarcane is the most efficient large-scale crop capable of supplying sufficient carbon substrate, in the form of sucrose, needed during fermentative feedstock production. however, sucrose metabolism in escherichia coli is not well understood because the two most common strains, e. coli k-12 and b, do not grow on sucrose. here, using a sucrose utilizing strain, e. coli w, we undertake an in-depth comparison of sucrose and glucose metabolism including growth kinetics, metabolite profiling, microa ... | 2014 | 25125039 |
| screening of lactic acid bacteria for their potential as microbial cell factories for bioconversion of lignocellulosic feedstocks. | the use of fossil carbon sources for fuels and petrochemicals has serious impacts on our environment and is unable to meet the demand in the future. a promising and sustainable alternative is to substitute fossil carbon sources with microbial cell factories converting lignocellulosic biomass into desirable value added products. however, such bioprocesses require availability of suitable and efficient microbial biocatalysts, capable of utilizing c5 sugars and tolerant to inhibitory compounds gene ... | 2014 | 24997803 |
| one-pot green synthesis of carbon dots by using saccharum officinarum juice for fluorescent imaging of bacteria (escherichia coli) and yeast (saccharomyces cerevisiae) cells. | we are reporting highly economical plant-based hydrothermal method for one-pot green synthesis of water-dispersible fluorescent carbon dots (cds) by using saccharum officinarum juice as precursor. the synthesized cds were characterized by uv-visible, fluorescence, fourier transform infrared (ft-ir), dynamic light scattering (dls), high-resolution transmission electron microscopic (hr-tem), and laser scanning confocal microscopic techniques. the cds are well dispersed in water with an average siz ... | 2014 | 24656348 |
| isolation of a novel peroxisomal catalase gene from sugarcane, which is responsive to biotic and abiotic stresses. | catalase is an iron porphyrin enzyme, which serves as an efficient scavenger of reactive oxygen species (ros) to avoid oxidative damage. in sugarcane, the enzymatic activity of catalase in a variety (yacheng05-179) resistant to the smut pathogen sporisorium scitamineum was always higher than that of the susceptible variety (liucheng03-182), suggesting that catalase activity may have a positive correlation with smut resistance in sugarcane. to understand the function of catalase at the molecular ... | 2014 | 24392135 |
| engineering and adaptive evolution of escherichia coli w for l-lactic acid fermentation from molasses and corn steep liquor without additional nutrients. | the d-lactic acid producing strain, escherichia coli hbut-d, was reengineered for l(+)-lactic acid fermentation by replacing the d-lactate dehydrogenase gene (ldha) with an l(+)-lactate dehydrogenase gene (ldhl) from pedicoccus acidilactici, followed by adaptive evolution in sucrose. the resulting strain, wyz-l, has enhanced expression of the sucrose operon (csca and csckb). in 100 g l(-1) of sucrose fermentation using mineral salt medium, wyz-l produced 97 g l(-1) of l(+)-lactic acid, with a yi ... | 2013 | 24063823 |
| molecular cloning and characterization of two pathogenesis-related β-1,3-glucanase genes scglua1 and scglud1 from sugarcane infected by sporisorium scitamineum. | two β-1,3-glucanase genes from sugarcane were cloned and characterized. they were all located in apoplast and involves in different expression patterns in biotic and abiotic stress. smut caused by sporisorium scitamineum is a serious disease in the sugarcane industry. β-1,3-glucanase, a typical pathogenesis-related protein, has been shown to express during plant-pathogen interaction and involves in sugarcane defense response. in this study, β-1,3-glucanase enzyme activity in the resistant variet ... | 2013 | 23842883 |
| repetitive succinic acid production from lignocellulose hydrolysates by enhancement of atp supply in metabolically engineered escherichia coli. | in this study, repetitive production of succinic acid from lignocellulose hydrolysates by enhancement of atp supply in metabolically engineered e. coli is reported. escherichia coli ba305, a pflb, ldha, ppc, and ptsg deletion strain overexpressing atp-forming phosphoenolpyruvate (pep) carboxykinase (pepck), produced a final succinic acid concentration of 83 g l(-1) with a high yield of 0.87 g g(-1) total sugar in 36 h of three repetitive fermentations of sugarcane bagasse hydrolysate. furthermor ... | 2013 | 23819977 |
| scmt2-1-3, a metallothionein gene of sugarcane, plays an important role in the regulation of heavy metal tolerance/accumulation. | plant metallothioneins (mts), which are cysteine-rich, low-molecular-weight, and metal-binding proteins, play important roles in detoxification, metal ion homeostasis, and metal transport adjustment. in this study, a novel metallothionein gene, designated as scmt2-1-3 (genbank accession number jq627644), was identified from sugarcane. scmt2-1-3 was 700 bp long, including a 240 bp open reading frame (orf) encoding 79 amino acid residues. a his-tagged scmt2-1-3 protein was successfully expressed i ... | 2013 | 23781509 |
| a 24.7-kda copper-containing oxidase, secreted by thermobifida fusca, significantly increasing the xylanase/cellulase-catalyzed hydrolysis of sugarcane bagasse. | thermobifida fusca is a moderately thermophilic soil bacterium belonging to actinobacteria. it has been known for its capability to degrade plant cell wall polymers except lignin and pectin. to know whether it can produce enzymes to facilitate lignin degradation, the extracellular proteins bound to sugarcane bagasse were harvested and identified by liquid chromatography tandem mass spectrometry. among the identified proteins, a putative copper-containing polyphenol oxidase of 241 amino acids, en ... | 2013 | 23377789 |
| expression and characterization of a gh39 β-xylosidase ii from caulobacter crescentus. | in the present work, the gene xynb2, encoding a β-xylosidase ii of the glycoside hydrolase 39 (gh39) family, of caulobacter crescentus was cloned and successfully overexpressed in escherichia coli dh10b. the recombinant protein (ccxynb2) was purified using nickel-sepharose affinity chromatography, with a recovery yield of 75.5 %. ccxynb2 appeared as a single band of 60 kda on a sodium dodecyl sulfate polyacrylamide gel and was recognized by a specific polyclonal antiserum. the predicted ccxynb2 ... | 2012 | 23054825 |
| metabolic engineering of klebsiella oxytoca m5a1 to produce optically pure d-lactate in mineral salts medium. | klebsiella oxytoca strains were constructed to produce optical pure d-lactate by ph-controlled batch fermentation in mineral salts medium. the alcohol dehydrogenase gene, adhe, and the phospho-transacetylase/acetate kinase a genes, pta-acka, were deleted from the wild type. kms002 (δadhe) and kms004 (δadhe δpta-acka) exhibited d-lactate production as a primary pathway for the regeneration of nad(+). both strains produced 11-13 g/l of d-lactate in medium containing 2% (w/v) glucose with yields of ... | 2012 | 22728200 |
| a novel dirigent protein gene with highly stem-specific expression from sugarcane, response to drought, salt and oxidative stresses. | dirigent and dirigent-like family proteins contain a number of proteins involved in lignification or in the response to pathogen infection and abiotic stress in plants. in the present study, a full-length cdna sequence of a dirigent-like gene designated scdir (genbank accession number jq622282) was obtained from sugarcane based on the stem full-length cdna library. the scdir gene was 819-bp long, including a 564-bp orf encoding 187 amino acid residues. the protein n-terminus contained signal pep ... | 2012 | 22696141 |
| effect of sugarcane fiber digestibility, conservation method and concentrate level on the ruminal ecosystem of beef cattle. | the aim of this study was to investigate the effects of sugarcane neutral detergent fiber digestibility (ndfd), conservation method, and concentrate level on the ruminal microbial population of steers. eight ruminal-cannulated nellore steers were distributed in two contemporary 4 × 4 latin square design with a 2 × 2 factorial arrangement of treatments. experiment 1: diets were formulated with 60% of concentrate level, and two sugarcane genotypes (high or low ndfd) either freshly cut or as silage ... | 2017 | 28265975 |
| parameters of fermentation and rumen microbiota of nellore steers fed with different proportions of concentrate in fresh sugarcane containing diets. | the objective of this study was to evaluate the effect of a fresh sugarcane-based diet and different roughage-to-concentrate ratios (70:30, 60:40, 40:60 and 20:80) on the rumen microbiota associated with rumen fermentation parameters and the intake and apparent digestibility of nutrients in nellore steers. eight rumen-cannulated nellore steers (331 ± 8 kg bw) were distributed in a double 4 × 4 latin square design balanced for the control of the residual effect. the ruminal ph decreased (p < 0.01 ... | 2016 | 27415825 |
| effect of fiber digestibility and conservation method on feed intake and the ruminal ecosystem of growing steers. | fiber digestibility is an important factor regulating dmi in ruminants. additionally, the ensiling process can also affect digestibility and chemical composition of the forage. the objective of this study was to investigate effects of sugarcane ndf digestibility (ndfd) and conservation method on intake, rumen kinetics, and the ruminal ecosystem of steers. eight ruminally cannulated nellore steers (275±22 kg bw) were used in a replicated 4×4 latin square design with a 2×2 factorial arrangement of ... | 2014 | 25414108 |
| chemiluminescent detection of fiji disease virus with biotinylated dna probes. | biotinylated fiji disease fijivirus specific cdna probes detected the presence of the virus in total nucleic acid extracts from infected sugarcane plants. hybridised biotinylated probes were detected with streptavidin-alkaline phosphatase conjugate and the light generating substrate amppd. samples were either blotted manually, or by alkaline capillary transfer using 100 mm naoh. transfer of nucleic acids to charge modified nylon with sodium hydroxide was superior to denaturation with glyoxal or ... | 1994 | 8031237 |
| detection of fiji disease virus in infected sugarcane by nucleic acid hybridization. | clones of fiji disease virus were obtained by cdna synthesis from isolated viral genomic double-stranded rna. nick-translated probes made from a mixture of clones specifically detected fiji disease virus in infected sugarcane galls, leaves and growing tips. this method for detection of fiji disease virus is extremely sensitive, and can be used as a rapid screening procedure for the virus with very small tissue samples. | 1986 | 3755138 |
| detection and identification of fiji disease virus in infected sugarcane by immunodiffusion, immuno-osmophoretic and enzyme-linked immunosorbent assays. | radial double-immunodiffusion, immuno-osmophoretic and enzyme-linked immunosorbent assay (elisa) methods have been compared for the detection of fiji disease virus (fdv) in infected sugarcane tissue extracts using an antiserum containing antibodies specific to fdv proteins and ds-rna. elisa was the most sensitive of these tests and detected only fdv-specific proteins byt not ds-rna. immuno-osmophoretic tests were less sensitive than elisa but detected both the protein and ds-rna antigens as dist ... | 1981 | 6795221 |
| striga biocontrol on a toothpick: a readily deployable and inexpensive method for smallholder farmers. | striga hermonthica (witchweed) is a parasitic weed that attacks and significantly reduces the yields of maize, sorghum, millet, and sugarcane throughout sub-saharan africa. low cost management methods such as hand weeding, short crop rotations, trap cropping, or conventional biocontrol have not been effective. likewise, striga-tolerant or herbicide-resistant maize cultivars are higher yielding, but are often beyond the economic means of sustenance farmers. the fungal pathogen, fusarium oxysporum ... | 2016 | 27551284 |
| development and application of a rapid and visual loop-mediated isothermal amplification for the detection of sporisorium scitamineum in sugarcane. | smut is a fungal disease with widespread prevalence in sugarcane planting areas. early detection and proper identification of sporisorium scitamineum are essential in smut management practices. in the present study, four specific primers targeting the core effector pep1 gene of s. scitamineum were designed. optimal concentrations of mg(2+), primer and bst dna polymerase, the three important components of the loop-mediated isothermal amplification (lamp) reaction system, were screened using a sin ... | 2016 | 27035751 |
| fusarium sacchari, a cause of mycotic keratitis among sugarcane farmers - a series of four cases from north india. | the two most common filamentous fungi causing mycotic keratitis are aspergillus and fusarium spp. around 70 fusarium spp. are involved in causing human infections. in this study, four cases of keratitis in sugarcane farmers in india are being reported, caused by the sugar cane pathogen fusarium sacchari, a species of the fusarium fujikuroi species complex. fusarial keratitis was established by potassium hydroxide/calcofluor white wet mounts and fungal culture of corneal scrapings on conventional ... | 2016 | 27292696 |
| rhamnolipid biosurfactant against fusarium sacchari--the causal organism of pokkah boeng disease of sugarcane. | pokkah boeng disease on sugarcane caused by the fungus fusarium sacchari results considerable damage to the crop leading to top rot, the most serious and advanced stage of pokkah boeng, where the growing point is killed and the entire top of the plant dies. in the present study, the effect of rhamnolipid biosurfactant as an antifungal agent against f. sacchari to control pokkah boeng disease was investigated. on the basis of surface tension reduction, 12 bacterial isolates were selected as poten ... | 2014 | 23687052 |
| in vitro generation of somaclonal variant plants of sugarcane for tolerance to fusarium sacchari. | key message : a combination of in vitro culture and mutagenesis using ethyl methanesulfonate (ems) followed by culture filtrate-mediated selection produced variant sugarcane plants tolerant and resistant to fusarium sacchari. eldana saccharina is a destructive pest of the sugarcane crop in south africa. fusarium sacchari png40 (a fungal strain harmful to e. saccharina) has the potential to be an endophytic biological control agent of the stalk borer. however, the fungus causes fusarium stalk rot ... | 2013 | 23090725 |
| mating populations of fusarium section liseola from rice, sugarcane and maize. | mating studies were conducted on 78 isolates of fusarium species section liseola from rice, sugarcane and maize. from the crosses with tester strains of gibberella fujikuroi species complex, 64.1% (50 out of 78 isolates) were cross-fertile with tester strains of mating populations a to e. the results of the mating studies showed that of the 50 isolates, 19 belonged to mating population a (gibberella moniliformis), 18 to mating population b (gibberella sacchari), 4 to mating population e (gibbere ... | 2011 | 24575220 |
| deciphering the transcriptomic response of fusarium verticillioides in relation to nitrogen availability and the development of sugarcane pokkah boeng disease. | pokkah boeng, caused by fusarium verticillioides, is a serious disease in sugarcane industry. the disease severity is related to the sugarcane genotype as well as environmental considerations, such as nitrogen application. the impact of the nitrogen source (ammonium sulfate, urea, or sodium nitrate) on sugarcane pokkah boeng disease and its pathogen was investigated in planta and fungal growth and sporulation production was measured in vitro. the results showed that ammonium and nitrate were ben ... | 2016 | 27434999 |
| fvbck1, a component of cell wall integrity map kinase pathway, is required for virulence and oxidative stress response in sugarcane pokkah boeng pathogen. | fusarium verticillioides (formerly f. moniliforme) is suggested as one of the causal agents of pokkah boeng, a serious disease of sugarcane worldwide. currently, detailed molecular and physiological mechanism of pathogenesis is unknown. in this study, we focused on cell wall integrity mapk pathway as one of the potential signaling mechanisms associated with pokkah boeng pathogenesis. we identified fvbck1 gene that encodes a map kinase kinase kinase homolog and determined that it is not only requ ... | 2015 | 26500635 |
| optimization of endoglucanase and xylanase activities from fusarium verticillioides for simultaneous saccharification and fermentation of sugarcane bagasse. | enzymatic hydrolysis is an important but expensive step in the production of ethanol from biomass. thus, the production of efficient enzymatic cocktails is of great interest for this biotechnological application. the production of endoglucanase and xylanase activites from f. verticillioides were optimized in a factorial design (2(5)) followed by a ccdr design. endoglucanase and xylanase activities increased from 2.8 to 8.0 u/ml and from 13.4 to 114 u/ml, respectively. the optimal ph and temperat ... | 2014 | 24170331 |