| involvement of n-acetyl-lactosamine-containing sugar structures in the liver metastasis of mouse colon carcinoma (colon 26) cells. | histochemical aspects of the process of experimentally induced metastasis were examined by light and electron microscopy with labelled lectins employed as a probe. mouse colon carcinoma cells (colon 26) were injected into the spleen of balb/c mice and liver metastasis was induced. among the lectins tested, erythrina cristagalli agglutinin (eca) stained the metastasized colon 26 cells strongly compared with the heterogeneous and faint staining in non-metastasized tumour foci in the spleen or in t ... | 1994 | 7532448 |
| ultrastructural identification of entorhinal cortical synapses in ca1 stratum lacunosum-moleculare of the rat. | the goal of the present study was to identify and characterize, at the electron microscopic level, the synapses formed by entorhinal cortical (ec) axons in the hippocampal ca1 stratum lacunosum-moleculare of the adult rat. phaseolus vulgaris leucoagglutinin was ionotophoresed at various loci throughout the mediolateral and dorsoventral extent of the ec to label ec-ca1 synapses. virtually all labeled synapses were asymmetric and axospinous. ec axons did not preferentially synapse with any particu ... | 1994 | 7534172 |
| distribution of spinal cord projections from the medullary subnucleus reticularis dorsalis and the adjacent cuneate nucleus: a phaseolus vulgaris-leucoagglutinin study in the rat. | the distribution and organization of descending spinal projections from the dorsal part of the caudal medulla were studied in the rat following injections of phaseolus vulgaris-leucoagglutinin into small areas of the subnucleus reticularis dorsalis (srd) and the adjacent cuneate nucleus (cu). the caudal aspect of the cu projected only to the dorsal horn of the ipsilateral cervical cord via the dorsal funiculus. these projections were mainly to laminae i, iv, and v. more ventrally located reticul ... | 1995 | 7536220 |
| evidence for a hypothalamothalamocortical circuit mediating pheromonal influences on eye and head movements. | a method for simultaneous iontophoretic injections of the anterograde tracer phaseolus vulgaris leukoagglutinin and the retrograde tracer fluorogold was used to characterize in the rat a hypothalamothalamocortical pathway ending in a region thought to regulate attentional mechanisms by way of eye and head movements. the relevant medial hypothalamic nuclei receive pheromonal information from the amygdala and project to specific parts of the thalamic nucleus reuniens and anteromedial nucleus, whic ... | 1995 | 7537378 |
| increase of satellite tobacco ringspot virus rna initiated by inoculating circular rna. | a small satellite rna of tobacco ringspot virus (strsv rna) generates circular and linear molecules of unit length and repetitive sequence, linear multimers during replication. the phosphodiester junction joining the unit satellite rna sequences in multimeric and circular rna resisted base-catalyzed cleavage in circles but not in linear dimers. we postulate that junctions of multimeric satellite rna form during synthesis of the polyribonucleotide chain, whereas those of circular rna result from ... | 1995 | 7538253 |
| inhibitory synaptic inputs to the oculomotor nucleus from vestibulo-ocular-reflex-related nuclei in the rabbit. | studies of the pathways involved in the vestibulo-ocular reflex have suggested that the projection from the superior vestibular nucleus to the ipsilateral oculomotor nucleus is inhibitory, whereas the medial vestibular nucleus, the abducens nucleus and the contralateral superior vestibular nucleus most likely exert excitatory effects on oculomotor neurons. in order to determine directly the termination pattern and the neurotransmitter of these afferents, we studied their input to the oculomotor ... | 1995 | 7538643 |
| cervical primary afferent input to vestibulospinal neurons projecting to the cervical dorsal horn: an anterograde and retrograde tracing study in the cat. | vestibulospinal neurons in the caudal half of the medial and descending vestibular nuclei terminate in the cervical spinal cord, not only in the ventral horn and intermediate zone but also in the dorsal horn. the purpose of the present study was to examine whether the areas containing these vestibulospinal neurons are reached by cervical primary afferents. in one group of experiments, wheat germ agglutinin-horseradish peroxidase conjugate and horseradish peroxidase were pressure injected into sp ... | 1995 | 7539013 |
| convection-enhanced distribution of large molecules in gray matter during interstitial drug infusion. | many novel experimental therapeutic agents, such as neurotrophic factors, enzymes, biological modifiers, and genetic vectors, do not readily cross the blood-brain barrier. an effective strategy to deliver these compounds to the central nervous system is required for their application in vivo. under normal physiological conditions, brain interstitial fluid moves by both bulk flow (convection) and diffusion. it has recently been shown that interstitial infusion into the white matter can be used to ... | 1995 | 7539062 |
| a modification of biotinylated dextran amine histochemistry for labeling the developing mammalian brain. | biotinylated dextran amine (bda) has proven to be an excellent anterograde tracer in adult mammalian brains, having some advantages over other anterograde tracers such as phaseolus vulgaris-leucoagglutinin (pha-l) and biocytin. however, results are inferior when bda is used in neonatal mammals. to improve the sensitivity and quality of bda labeling in neonatal mammalian brains, the tetramethylbenzidine-sodium tungstate (tmb-st) method for horseradish peroxidase (hrp) histochemistry was modified ... | 1995 | 7540703 |
| patterning of local intracortical projections within the vibrissae representation of rat primary somatosensory cortex. | anterograde and retrograde tracing with biotinylated dextran amine and phaseolus vulgaris leukoagglutinin was used to assess projection patterns within the vibrissae representation of the rat's primary somatosensory cortex (s-i). large and small injections of either tracer into the center of the vibrissae representation yielded dense anterograde and retrograde labelling throughout much of the tangential extent of the vibrissae representation within s-i. in all layers, the pattern and extent of r ... | 1995 | 7541807 |
| innervation of motoneurons based on dendritic orientation. | 1. the distribution of terminals from vestibulospinal (vs) axons on the dendritic trees of neck motoneurons was determined by combining the anterograde transport of phaseolus vulgaris leucoagglutinin (pha-l) with intracellular staining techniques and three-dimensional reconstruction methods. 2. contacts between vs axon terminals and dendrites were arranged in a nonuniform pattern that depended on the orientation (i.e., rostro-caudal vs. dorsolateral) of the dendrites. 3. this innervation pattern ... | 1995 | 7541831 |
| lectin and immunohistochemical comparison of glycoconjugates in the conjunctiva of patients with and without exfoliation syndrome. | aims--the study was carried out to search for labelling similar to that of intraocular exfoliation material in the conjunctiva by light microscopy using lectin and immunohistochemistry. methods--ten formalin fixed and paraffin embedded conjunctival biopsy specimens both from patients with and without exfoliation syndrome were studied with a panel of 11 lectins and with three monoclonal antibodies to the hnk-1 carbohydrate epitope, all of which react with intraocular exfoliation material. results ... | 1995 | 7542024 |
| growth, hormonal status and protein turnover in rats fed on a diet containing peas (pisum sativum l.) as the source of protein. | the inclusion of peas (pisum sativum l.) as the source of protein in the diet of growing rats brings about a reduction in growth rate as well as the impairment in the liver, muscle and spleen weights as compared with casein fed controls. also, a fall in plasma glucose, triglycerides and protein was observed in the legume fed animals, while no changes in cholesterol levels were found. furthermore, the rats fed on the diet containing peas showed lower levels of plasma insulin, corticosterone, igf- ... | 1995 | 7544899 |
| nitric oxide-synthesising neurons in the central subnucleus of the nucleus tractus solitarius provide a major innervation of the rostral nucleus ambiguus in the rabbit. | we describe an intramedullary nitric oxide synthase (nos) neural pathway that projects from the nucleus tractus solitarius (nts) to the rostral nucleus ambiguus (na) in the rabbit. with the use of nadph diaphorase histochemistry and nos immunohistochemistry, a compact group of nos-positive perikarya was identified in the central subnucleus of the nts dorsomedial to the tractus solitarius and rostral to the obex. a dense network of nos terminals was seen in the rostral na. we investigated whether ... | 1995 | 7545701 |
| digestive adaptations of rats given white bread and cooked haricot beans (phaseolus vulgaris): large-bowel fermentation and digestion of complex carbohydrates. | two experiments were carried out to examine the short (1-3 d) and medium (14 d) term adaptations of rat large-bowel (lb) fermentation to alterations in substrate supply brought about by including cooked haricot beans (phaseolus vulgaris) in diets based on white bread. changes in organic matter (om) flow from the ileum occurred within 1 d and were stable for 14 d but the pattern of caecal short-chain fatty acids took much longer to stabilize with considerable increases in butyrate between 1 and 3 ... | 1995 | 7547852 |
| cis elements and potential trans-acting factors for the developmental regulation of the phaseolus vulgaris chs15 promoter. | a nuclear factor (sbf-1) has previously been identified in phaseolus vulgaris l. (bean) suspension cell nuclear extracts that binds in vitro to three dnase i-footprinted elements (sbf-1 boxes i, ii, and iii, 5' to 3') in the 5' region of the bean chs15 (chalcone synthase) gene promoter. to define the functional role of the three sbf-1 boxes in development, we examined transgenic tobacco plants carrying a series of nested chs15 promoter-beta-glucuronidase (gus) fusions for gus activity by histoch ... | 1995 | 7548834 |
| pvalf, an embryo-specific acidic transcriptional activator enhances gene expression from phaseolin and phytohemagglutinin promoters. | mutations in vp1 and abl3 genes of maize and arabidopsis lead to drastic reductions in the synthesis of a subset of maturation-specific products including seed storage proteins. gene phaseolus vulgaris abl3-like factor (pvalf), whose protein product is similar to the abl3 and vp1 proteins, has been cloned. here, it is shown that pvalf positively regulates phaseolin and phytohemagglutinin (pha-l) promoters in particle bombardment assays. pvalf mrna expression is embryo-specific and temporally com ... | 1995 | 7550372 |
| efferent projections from the region of the medial zona incerta containing a13 dopaminergic neurons: a pha-l anterograde tract-tracing study in the rat. | potential efferent projections of a13 dopaminergic (da) neurons were identified in the present study by examining the distribution of labelled fibers following iontophoretic injection of the anterogradely transported lectin phaseolus vulgaris leucoagglutinin (pha-l) into the medial zona incerta (mzi), the region of the diencephalon containing a13 da neuronal perikarya. one week after injection, pha-l labelled fibers were found throughout the brain with the heaviest labelling occurring ipsilatera ... | 1995 | 7552247 |
| efferent projections from the retrochiasmatic area to the median eminence and to the pars nervosa of the hypophysis with special reference to the a15 dopaminergic cell group in the sheep. | anterograde tracers, viz. phaseolus vulgaris leucoagglutinin and fluorescein dextran, were used in conjunction with tyrosine hydroxylase immunohistochemistry to study the projections of the a15 dopaminergic cell group towards the median eminence and pituitary in sheep. after injection of the tracers in the retrochiasmatic area, which contains the cell group a15, fibres containing anterograde tracer were observed in the internal zone of the median eminence and in the pars nervosa of the pituitary ... | 1995 | 7553775 |
| neocortical grafts placed in the infarcted brain of adult rats: few or no efferent fibers grow from transplant to host. | the present study examines the capacity of fetal neocortical grafts placed in a brain infarct to exchange axonal projections with the host brain. five to 7 days after a middle cerebral artery occlusion in adult spontaneously hypertensive rats, dissociated neocortical primordium from fetuses of gestational age 15-16 days was implanted into the infarcted area. four to 11 months later, the neural tracers phaseolus vulgaris-leucoagglutinin and fluoro-gold were injected in the grafts and host neocort ... | 1995 | 7556547 |
| [h2o2 generation and human neutrophil aggregation as affected by lectins]. | the effects of eight plant lectins on human neutrophils aggregation and h2o2 release were studied. both processes were stimulated by lectins from viscum album, triticum vulgaris, phaseolus vulgaris and canavalia ensiformis while lectins from solanum tuberosum, sambucus nigra and glycine mas displayed only aggregating activity and arachis hypogaea lectin was not effective. heating (46 degrees c) and uv-radiation during 4-5 min were found to suppress completely h2o2 release from neutrophils keepin ... | 1995 | 7557235 |
| premotor neurons for trigeminal motor nucleus neurons innervating the jaw-closing and jaw-opening muscles: differential distribution in the lower brainstem of the rat. | the distribution of premotor neurons for trigeminal motor nucleus neurons innervating the jaw-closing and jaw-opening muscles was examined in the lower brainstem of the rat by using retrograde and anterograde labeling techniques. first, fluorogold, a fluorescent retrograde tracer, was injected into the dorsolateral or ventromedial division of the trigeminal motor nucleus, each of which contains motoneurons innervating the jaw-closing or jaw-opening muscles, respectively. second, phaseolus vulgar ... | 1995 | 7560267 |
| species limits in rhizobium populations that nodulate the common bean (phaseolus vulgaris). | evolutionary genetic relationships among 146 bean-nodulating rhizobium strains, including 94 field isolates from three localities in colombia and 36 strains from mexico, were examined by multilocus enzyme electrophoresis and restriction fragment length polymorphism analysis of a pcr-amplified 260-bp segment of the 16s rrna gene. seventy-five electrophoretic types (ets), corresponding to multilocus enzyme genotypes, were identified, including a genotypically diverse group of 18 ets in colombia th ... | 1995 | 7574588 |
| purified pseudomonas aeruginosa pa-i lectin induces gut growth when orally ingested by rats. | the effects of pa-i lectin isolated from the human pathogen pseudomonas aeruginosa upon cellular metabolism in vivo have been studied using the rat gut as a model system. orally ingested pa-i lectin stimulated metabolic activity and induced polyamine accumulation and growth in the small intestine, caecum and colon. the nature and extent of the changes induced by pa-i lectin were similar to those caused by dietary kidney bean lectin and were likely to lead to impaired epithelial cell function and ... | 1995 | 7581270 |
| high frequency of conjugation versus plasmid segregation of rp1 in epiphytic pseudomonas syringae populations. | the maintenance and transfer of the broad host-range plasmid rp1 in epiphytically growing populations of pseudomonas syringae was monitored in the phyllosphere of bush bean (phaseolus vulgaris). when foliage was inoculated with plasmid-containing bacteria, the plasmid was lost from the majority of the cells within 2 d but was stably maintained in 0.8% of the population. a high frequency of conjugation between added donors and recipients was observed under high humidity conditions. in 1 d, the nu ... | 1995 | 7582033 |
| connections of the lateral reticular nucleus to the lateral vestibular nucleus in the rat. an anterograde tracing study with phaseolus vulgaris leucoagglutinin. | efferent projections from the lateral reticular nucleus in the rat were investigated with anterograde transport of phaseolus vulgaris leucoagglutinin. besides the well known mossy fibre connections to the cerebellar cortex and collaterals to the cerebellar nuclei, a substantial bilateral projection to the lateral vestibular nucleus was found. terminal arborizations found within this nucleus appeared to detach from the reticulocerebellar fibres in the cerebellar white matter and enter the lateral ... | 1995 | 7582116 |
| direct projection from the suprachiasmatic nucleus to hypophysiotrophic corticotropin-releasing factor immunoreactive cells in the paraventricular nucleus of the hypothalamus demonstrated by means of phaseolus vulgaris-leucoagglutinin tract tracing. | the diurnal rhythm of the activity of the hypothalamo-pituitary-adrenal axis is generated by the circadian pacemaker located in the suprachiasmatic nuclei (scn). however, the neuronal circuit connecting the scn with the neurosecretory corticotropin-releasing factor (crf) neurons in the paraventricular nucleus of the hypothalamus is not clear. to investigate the existence of a direct link between the scn and the crf neurons in the pvn we combined microiontopheretic injections of the anterograde t ... | 1995 | 7583205 |
| estrogen-receptive neurons in the anteroventral periventricular nucleus are synaptic targets of the suprachiasmatic nucleus and peri-suprachiasmatic region. | the anteroventral periventricular nucleus (avpv) in the rat preoptic area is a key site underlying control of the steroid dependent preovulatory gonadotropin surge. estrogen and progesterone receptor-containing neurons in the preoptic/hypothalamic continuum, particularly those in the avpv, are believed to transduce steroidal signals and, in turn convey this information to the lhrh system, which lacks steroid receptors. in addition to the influence of the gonadal steroids, the precise timing of t ... | 1995 | 7583329 |
| glycoconjugate expression of chondrocytes and perichondrium during hyaline cartilage development in the rat. | alterations in the expression of glycoconjugate structures during cartilage development in the chondrocranium, nasal skeleton, meckel's cartilage, limb buds, vertebral bodies and ribs were investigated comparatively in 13 to 21-d-old rat embryos. the binding patterns of 24 biotinylated lectins were analysed in serial sections and compared with results obtained using histochemical methods. proteoglycan distribution, assessed by conventional staining procedures, was not associated with lectin bind ... | 1995 | 7591987 |
| effects of astrocyte implantation into the hemisected adult rat spinal cord. | morphological and biochemical methods were applied to assess the effects of implanting cultured astrocytes into the hemisected adult rat spinal cord. astrocytes were purified from neonatal rat cortex and introduced into the lesioned spinal cord either in suspension injection or cultured on gelfoam first. the control groups were rats which had hemisection with injection of culture media or with gelfoam grafted alone. at various time points after surgery (two weeks to two months), the spinal cord ... | 1995 | 7617172 |
| phylogenetic relationships and host range of rhizobium spp. that nodulate phaseolus vulgaris l. | we determined the nucleotide sequences of 16s rrna gene segments from five rhizobium strains that have been isolated from tropical legume species. all share the capacity to nodulate phaseolus vulgaris l., the common bean. phylogenetic analysis confirmed that these strains are of two different chromosomal lineages. we defined the host ranges of two strains of rhizobium etli and three strains of r. tropici, comparing them with those of the two most divergently related new strains. twenty-two of th ... | 1995 | 7618891 |
| developmentally-regulated lectin binding in the embryonic mouse telencephalon. | cell-surface carbohydrate epitopes are important determinants in cell-cell and cell-matrix interactions, and oligosaccharide groups are structural components of many growth factor receptors and cell adhesion molecules. these epitopes may participate in the regulation of stem cell proliferation and differentiation during central nervous system development. to further understand these cellular phenomena, it is important to define the changes in neuroepithelial cell-surface carbohydrate expression ... | 1995 | 7620904 |
| glycaemic response in normal subjects to five different legumes commonly used in the philippines. | five legumes including chick pea (cicer arietinum linn), pigeon pea (cajanus cajan linn. huth.), black bean (phaseolus vulgaris linn), mung bean (phaseolus areus roxb) and white bean (phaseolus vulgaris linn) were cooked and tested for blood glucose response among healthy human volunteers. the blood glucose response to all legumes was significantly lower compared to bread. the glycaemic response to chick pea was significantly lower (p < or = 0.01) than that to black bean, pigeon pea and mung bea ... | 1995 | 7621088 |
| vibrissal motor cortex in the rat: connections with the barrel field. | the flow of information in the sensorimotor cortex may determine how somatic information modulates motor cortex neuronal activity during voluntary movement. electrophysiological recordings and neuroanatomical tracing techniques were used to study the connections between the primary somatosensory cortex (si) and the vibrissal representation of the primary motor cortex (mi) in rodents. intracortical microstimulation (icms) was applied to the vibrissal region of the motor cortex to identify a site ... | 1995 | 7621940 |
| intrinsic connections of the rat amygdaloid complex: projections originating in the lateral nucleus. | the amygdaloid complex receives sensory information from a variety of sources. a widely held view is that the amygdaloid complex utilizes this information to orchestrate appropriate species-specific behaviors to ongoing experiences. relatively little is known, however, about the circuitry through which information is processed within the amygdaloid complex. the lateral nucleus is the major recipient of extrinsic sensory information and is the origin of many intra-amygdaloid projections. in this ... | 1995 | 7629320 |
| descending efferent connections of the sub-pallidal areas in the cat: projections to the lateral habenula. | the descending efferent connections of the sub-pallidal areas to the lateral habenula were investigated in the cat using phaseolus vulgaris leucoagglutinin (pha-l) as an anterograde tracer. several injections of pha-l were made in various regions of the feline sub-pallidal regions. subsequently, the distribution of anterogradely labelled fibres in the lateral habenula was charted. pha-l injections into the rostral part of the sub-pallidal regions resulted in a limited number of labelled fibres i ... | 1995 | 7632903 |
| rhizobium tropici nodulation factor sulfation is limited by the quantity of activated form of sulfate. | rhizobium tropici is a broad host-range symbiont of phaseolus vulgaris. this bacterium produces a mixture of sulfated and non-sulfated n-methylated pentameric nodulation (nod) factors. to understand the genetic bases of the partial sulfation of r. tropici nod factors, which might be involved in the broad host-range of this species, we introduced in r. tropici cfn299 the recombinant plasmid pgmi515 carrying a set of nodulation (nod) genes of r. meliloti, including those involved in the sulfation ... | 1995 | 7635216 |
| degradation of transport-competent destabilized phaseolin with a signal for retention in the endoplasmic reticulum occurs in the vacuole. | to understand how plant cells exert quality control over the proteins that pass through the secretory system we examined the transport and accumulation of the bean (phaseolus vulgaris l.) vacuolar storage protein phaseolin, structurally modified to contain a helix-breaking epitope and carboxyterminal hdel, an endoplasmic reticulum (er)-retention signal. the constructs were expressed in tobacco (nicotiana tabacum l.) with a seed-specific promoter. the results show that phaseolin-hdel accumulates ... | 1995 | 7647686 |
| the fusarium solani gene encoding kievitone hydratase, a secreted enzyme that catalyzes detoxification of a bean phytoalexin. | among the antimicrobial phytoalexins produced by phaseolus vulgaris (french bean) is the prenylated isoflavonoid, kievitone. the bean pathogen, fusarium solani f. sp. phaseoli, secretes a glycoenzyme, kievitone hydratase (ec 4.2.1.95), which catalyzes conversion of kievitone to a less toxic metabolite. among f. solani strains, those that are highly virulent to p. vulgaris also produce kievitone hydratase constitutively, suggesting that the enzyme is a virulence factor. based on the n-terminal am ... | 1995 | 7655061 |
| identification and characterization of a rhizobium leguminosarum bv. phaseoli gene that is important for nodulation competitiveness and shows structural homology to a rhizobium fredii host-inducible gene. | dna sequence analysis of a 1.4-kb sali-hindiii segment located approximately 2 kb upstream of the rhizobium leguminosarum bv. phaseoli syrm gene revealed the presence of an open reading frame (orf3) encoding a putative 295-amino acid polypeptide with a molecular mass of 33,401 da. orf3 is homologous to a r. fredii host-inducible gene. the proteins encoded by r. l. bv. phaseoli orf3 and by the r. fredii host-inducible gene share 37% sequence identity. in contrast to the r. fredii host-inducible g ... | 1995 | 7655066 |
| selective rostral transection of the fornix spares the hippocampal commissural pathway in the rat: a phaseolus vulgaris leucoagglutinin tracing study. | this study describes an approach for disconnecting the septal region from the hippocampus by fimbria-fornix lesions while sparing the commissural projections. after a frontal cut through the rostral fornix, commissural fibres were labelled with the anterograde tracer phaseolus vulgaris leucoagglutinin. the commissural fibre bundle located in the posterior-basal fornix (ventral hippocampal commissure) remained unaffected by the rostral fornix transection, whereas the absence of septal fibres in t ... | 1995 | 7672017 |
| different climbing fibres innervate separate dendritic regions of the same purkinje cell in hypogranular cerebellum. | electrophysiological experiments have shown that in hypogranular cerebella the purkinje cells are innervated by several climbing fibres. the aim of this paper is to provide morphological evidence for this multiple innervation and to describe the topographical distribution of the different climbing fibres onto the somadendritic region of the purkinje cell. experiments have been performed in hypogranular adult wistar rats lesioned during the first postnatal week by methylazoxymethanol (mam) or by ... | 1995 | 7673475 |
| internal connections in the rostral ventromedial medulla of the rat. | physiological and pharmacological data suggest that the rostral ventromedial medulla (rvm) is an important site where integration between somatic and visceral functions might occur. the aim of the present study was to describe the interconnections between various nuclei of the rostral ventromedial medulla and thus reveal the possible anatomical basis for such functional interactions. the topography of anterogradely labelled internal projections was examined following iontophoretic microinjection ... | 1995 | 7673601 |
| efferent projections from the parabrachial nucleus demonstrated with the anterograde tracer phaseolus vulgaris leucoagglutinin. | efferent projections from the parabrachial complex (pbn) were studied in the rat using the anterograde tracer, phaseolus vulgaris leucoagglutinin (pha-l). projections to the hypothalamus (ventromedial, dorsomedial, paraventricular, and supraoptic nuclei) originate primarily in the lateral pbn (1pbn). the amygdalar central nucleus (ace) receives strong projections from all parts of the pbn although the external 1pbn projects primarily to the lateral ace. whereas the projections to the lateral bed ... | 1993 | 7678381 |
| fine structure of the dorsal cap of the inferior olive and its gabaergic and non-gabaergic input from the nucleus prepositus hypoglossi in rat and rabbit. | the dorsal cap of the inferior olive is involved in the control of eye movements and is excited by inputs from the midbrain. in the present study we attempted to determine the inhibitory input to this nucleus in rat and rabbit. the projection from the nucleus prepositus hypoglossi to the dorsal cap was studied in the light microscope by anterograde tracing of phaseolus vulgaris-leucoagglutinin and lesion-induced depletion of glutamic acid decarboxylase immunoreactivity, and in the electron micro ... | 1993 | 7679420 |
| topography of descending projections from the inferior colliculus to auditory brainstem nuclei in the rat. | we examined the organization of descending projections from the inferior colliculus (ic) to auditory brainstem nuclei and to pontine and reticular nuclei in the rat by employing the anterograde axonal tracer phaseolus vulgaris-leucoagglutinin (pha-l). small pha-l injections into cytologically defined subnuclei of the ic revealed that each subnucleus has a unique pattern of efferent projections. the central nucleus of the ic projects in a topographic order to the dorsal nucleus of the lateral lem ... | 1993 | 7680052 |
| absence of serotonergic innervation from raphe nuclei in rat cerebral blood vessels--i. histological evidence. | anterograde tracing from dorsal raphe neurons by phaseolus vulgaris leucoagglutinin and serotonin immunocytochemistry revealed no serotonergic projections from raphe nuclei to cerebral pial vessels in the rat. however, cerebrovascular nerve fibres, mainly located in major pial arteries, were immunoreactive to tryptophan-5-hydroxylase antibodies as previously shown by others. it thus seems that the rate-limiting enzyme catalysing the biosynthesis of serotonin, tryptophan-5-hydroxylase, is present ... | 1993 | 7680791 |
| calretinin is present in non-pyramidal cells of the rat hippocampus--iii. their inputs from the median raphe and medial septal nuclei. | the subcortical innervation of a recently described subpopulation of non-pyramidal neurons, containing the calcium binding protein, calretinin, was investigated in the rat hippocampus using the anterograde tracer phaseolus vulgaris-leucoagglutinin and double immunocytochemistry for calretinin and serotonin at the light and electron microscopic levels. our results show that the gabaergic component of the septohippocampal pathway and the serotonergic raphe afferents establish multiple synaptic con ... | 1993 | 7680801 |
| brainstem afferents to the rostral (juxtafacial) nucleus paragigantocellularis: integration of exteroceptive and interoceptive sensory inputs in the ventral tegmentum. | the rostral pole of the nucleus paragigantocellularis (pgi), termed juxtafacial pgi, lies medially adjacent to the facial nucleus and lateral to the pyramidal tract and the nucleus gigantocellularis pars alpha in the caudal ventral pons. this narrow region of the ventral pontine reticular formation is an afferent to the nucleus locus coeruleus, and contains neurons which have been implicated in pain processing, cardiovascular regulation, respiratory control and arousal. here, we studied brainste ... | 1993 | 7680934 |
| functional and anatomical organization of cardiovascular pressor and depressor sites in the lateral hypothalamic area. ii. ascending projections. | microinjections of l-glutamate or d,l-homocysteic acid were used to stimulate cell bodies in the region of the lateral hypothalamic area (lha) selectively. subsequent iontophoretic injections of phaseolus vulgaris-leucoagglutinin or pressure injections of wheat germ agglutinin-horseradish peroxidase were made into regions containing identified pressor and depressor sites and their connections with the forebrain and cerebral cortex were traced. the results indicate that decreases in blood pressur ... | 1993 | 7682225 |
| demonstration of axon terminals of projection fibers from the periaqueductal gray onto neurons in the nucleus raphe magnus which send their axons to the trigeminal sensory nuclei. | axon terminals of projection fibers from the periaqueductal gray (pag) onto neurons in the nucleus raphe magnus (nrm) sending their axons to the principal sensory trigeminal nucleus (vp) or caudal spinal trigeminal nucleus (vc) were demonstrated light microscopically: in the rats which were injected with phaseolus vulgaris leucoagglutinin (pha-l) and wheat germ agglutinin conjugated to horseradish peroxidase (wga-hrp) respectively into the pag and trigeminal sensory complex (vp or vc), presumed ... | 1993 | 7684309 |
| ventral subicular interaction with the hypothalamic paraventricular nucleus: evidence for a relay in the bed nucleus of the stria terminalis. | the axonal projections of the ventral subiculum to the bed nucleus of the stria terminalis (bst) were examined in the rat with the anterograde neuronal tracer phaseolus vulgaris-leucoagglutinin (pha-l). axons originating in the ventral subiculum coursed to the bst through either the fimbria-fornix, or a pathway involving the stria terminalis via the amygdala. ventral subicular axons gave rise to dense terminal networks that were preferentially distributed in medial and ventral subregions of the ... | 1993 | 7685778 |
| innervation of the amygdaloid complex by catecholaminergic cell groups of the ventrolateral medulla. | the projections to the amygdaloid complex (amg), originating in the catecholaminergic cell groups of the ventrolateral medulla (vlm), were studied in the rat by using either the retrograde tracer fluoro-gold (fg) or the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l) in combination with tyrosine hydroxylase (th) and/or phenylethanolamine-n-methyltransferase (pnmt) immunohistochemistry. in the first series of experiments, injections of fg were made into regions of the central nucleu ... | 1993 | 7685779 |
| local origins of some gabaergic projections to the paraventricular and supraoptic nuclei of the hypothalamus in the rat. | axonal transport and immunohistochemical methods were used to characterize the organization of glutamic acid decarboxylase-immunoreactive (gad-ir) projections to the paraventricular (pvh) and supraoptic (so) nuclei of the hypothalamus in the rat. in line with prior reports, gad-ir varicosities were found to be densely and quite uniformly distributed throughout the hypothalamus, including the pvh and the so. nonetheless, the periventricular part of the pvh was consistently found to contain a disp ... | 1993 | 7685780 |
| direct projections from the midbrain periaqueductal gray and the dorsal raphe nucleus to the trigeminal sensory complex in the rat. | it is well known that analgesia can be produced by stimulation of the midbrain periaqueductal gray and dorsal raphe nucleus. this stimulation-produced analgesia may operate, at least partly, through direct projections to nociceptors in the primary sensory nuclei. in the present study, direct projections from the midbrain periaqueductal gray and dorsal raphe nucleus to the trigeminal sensory complex were studied in the rat. after injection of phaseolus vulgaris leucoagglutinin into the periaquedu ... | 1993 | 7687754 |
| the projections of noradrenergic neurons in the a5 catecholamine cell group to the spinal cord in the rat: anatomical evidence that a5 neurons modulate nociception. | brainstem noradrenergic neurons located in the a5, a6, and a7 catecholamine cell groups provide the entire noradrenergic innervation of the spinal cord. we have previously demonstrated that noradrenergic neurons in the a6 and a7 cell groups innervate the ventral and dorsal horns, respectively. since the specific spinal cord terminations of the a5 cell group have not been clearly delineated, the present experiments were designed to trace the projections from this noradrenergic cell group to the s ... | 1993 | 7689410 |
| postsynaptic targets of gabaergic hippocampal neurons in the medial septum-diagonal band of broca complex. | the termination pattern of hippocamposeptal nonpyramidal cells was investigated by injecting phaseolus vulgaris leucoagglutinin (phal) into stratum oriens of the ca1 region. electron microscopic analysis showed that the majority of the anterogradely labeled boutons formed symmetric synapses with dendrites and occasionally with cell bodies located in the medial septum-diagonal band of broca complex. we have demonstrated with postembedding gaba immunocytochemistry that the majority of phal-labeled ... | 1993 | 7690065 |
| hierarchical organization of areas in rat visual cortex. | to test the hypothesis that areas within rat visual cortex are organized in a multilevel hierarchy, we have employed phaseolus vulgaris leucoagglutinin as an anterograde axonal tracer to visualize the laminar patterns of connections between different cortical areas. for identification of cortical areas, we used a combination of markers that included callosal connections, the patterns of inputs and outputs to ipsilateral cortical and subcortical targets, and geographical location. projections fro ... | 1993 | 7690066 |
| the anterograde neuroanatomical tracer biotinylated dextran-amine: comparison with the tracer phaseolus vulgaris-leucoagglutinin in preparations for electron microscopy. | we investigated the properties of biotinylated dextran-amine (bda) as a neuroanatomical tracer at the electron microscopic level and we compared the results with those obtained previously with another tracer, the lectin phaseolus vulgaris-leucoagglutinin (pha-l). bda was injected into various brain areas of rats. following survival and fixation, vibratome sections were cut, subjected to a freeze-thaw treatment, and incubated overnight with avidin-biotin complex (abc). following reaction with dia ... | 1993 | 7690870 |
| ultrastructure and synaptic targets of the raphe-nigral projection in the rat. | a major input to the substantia nigra is from the 5-hydroxytryptamine-containing neurons in the dorsal raphe nucleus. in order to examine the morphology and distribution of this projection, rats were given injections of the anterograde tracers, phaseolus vulgaris-leucoagglutinin or biocytin, in the dorsal raphe nucleus and the substantia nigra was examined at both the light and electron microscopic levels. in addition, sections of the substantia nigra were immunostained for 5-hydroxytryptamine a ... | 1993 | 7690910 |
| innervation of serotonergic medullary raphe neurons from cells of the rostral ventrolateral medulla in rats. | the rostral ventral medulla has been shown to consist of three distinct subregions: the midline or raphé region, the lateral paragigantocellular-gigantocellular region and the rostro-ventrolateral reticular nucleus. all three regions have been shown to contribute to central vaso-regulation and to project towards sympathetic preganglionic neurons of the thoracic spinal cord. therefore it is of particular interest to describe the interconnections between the three regions and to see if local affer ... | 1993 | 7692351 |
| descending projections from the medullary dorsal reticular nucleus make synaptic contacts with spinal cord lamina i cells projecting to that nucleus: an electron microscopic tracer study in the rat. | an ultrastructural study is made of the synaptic contacts occurring between structures labelled anterogradely and retrogradely in the superficial dorsal horn following injections of cholera toxin subunit b or horseradish peroxidase in the dorsal reticular nucleus of the medulla oblongata of the rat. both tracers revealed labelled axonal boutons in lamina i with round synaptic vesicles and a few large granular vesicles making asymmetrical synaptic contacts upon labelled somata and dendrites. afte ... | 1993 | 7694179 |
| efferent connections of the hypothalamic "grooming area" in the rat. | the efferent connections of the hypothalamic area, where grooming can be elicited by local electrical stimulation or injection of various substances, were studied using iontophoretic injections of phaseolus vulgaris leucoagglutinin. this hypothalamic "grooming area" consists of parts of the hypothalamic paraventricular nucleus and of the dorsal hypothalamic area. the specificity of these efferents for the hypothalamic "grooming area" was investigated by comparison with efferents of hypothalamic ... | 1993 | 7694185 |
| demonstration of habenular neurons which receive afferent fibers from the nucleus accumbens and send their axons to the midbrain periaqueductal gray. | after injecting phaseolus vulgaris leucoagglutinin (pha-l) and wheat germ agglutinin conjugated with horseradish peroxidase (wga-hrp), respectively, into the medial part of the nucleus accumbens (na) and the midbrain periaqueductal gray including the dorsal raphe nucleus (pag/dr) on one side of single rat brains, we observed that axon terminals labeled anterogradely with pha-l made possible synaptic contact with neurons in the habenula (hb) which were retrogradely labeled with wga-hrp. these hb ... | 1993 | 7694201 |
| medullary synaptic inputs to thyrotropin-releasing hormone (trh)-containing neurons in the hypothalamus: an ultrastructural study combining wga-hrp anterograde tracing with trh immunocytochemistry. | ascending projections from the a1/c1 cell group and from the a2 cell group in the medulla oblongata was studied in the light microscope by anterograde tracing of phaseolus vulgaris leucoagglutinin and in the electron microscope by anterograde tracing of wheat germ agglutinin-coupled horseradish peroxidase (wga-hrp) combined with thyrotropin-releasing hormone (trh) immunocytochemistry in the hypothalamic paraventricular nucleus (pvn). wga-hrp-labeled axon terminals originating from neurons in the ... | 1993 | 7694778 |
| bacterial translocation in the rat model of lectin induced diarrhoea. | red kidney beans were fed to weanling long-evans rats to cause diarrhoea (mean (sd) faecal wet weight: 2.66 (0.73) g/day in six rats fed beans v 1.12 (0.47) g/day in six control rats, p < 0.01) and increased faecal energy loss (4.87 (0.41) v 2.14 (0.23) kcal/day, p < 0.01). in addition, the rats fed beans had heavier small intestines (80.6 (4.6) v 51.9 (8.4) g/kg body weight, p < 0.01), heavier mesenteric lymph nodes (0.72 (0.27) v 0.08 (0.08) g/kg body weight, p < 0.05), and translocation of in ... | 1995 | 7698696 |
| efferents of frontal or occipital cortex grafted into adult rat's motor cortex. | phaseolus vulgaris leucoagglutinin (pha-l) was used to examine the efferent connectivity of embryonic (e16) frontal (homotopic) or occipital (heterotopic) neocortical transplants placed into--or in the vicinity of--lesion cavities made in the frontal cortex of adult recipients. homotopic transplants projected towards the host sensorimotor cortex and, in most cases, into the lateral caudate-putamen (cpu). heterotopic transplants projected into the anterior cingulate cortex and, in most cases, dis ... | 1994 | 7700592 |
| terminal morphology and distribution of corticothalamic fibers originating from layers 5 and 6 of cat primary auditory cortex. | two types of terminations were observed on corticothalamic fibers arising from cells in different layers of cat auditory cortex. injections of the anterograde tracers phaseolus vulgaris leucoagglutinin (pha-l) or biocytin were made into single cortical loci that included both superficial layer 5 (5a) and layer 6 in the primary auditory cortex (ai). these resulted in labeling of terminal fibers with small (approximately 1 micron) and large (approximately 2 microns) boutons in the medial geniculat ... | 1994 | 7703690 |
| purification and characterization of phaseolus vulgaris alpha-d-galactosidase isozymes. | a highly purified preparation of alpha-d-galactosidase [e.c. 3.2.1.22] isozymes was obtained from phaseolus vulgaris (pinto bean) seeds by extraction, salt precipitation, ion exchange, and affinity chromatography. the final preparation was homogeneous by sds-page but revealed isozymes of relative mass of 38.3 and 39.6 kda. the n-terminal sequence for both isozymes was identical, langlakt (one letter code for amino acids). relative native molecular mass was estimated at 149.3 kda by sephacryl s-2 ... | 1994 | 7703901 |
| immunostaining and interphase arrangement of field bean kinetochores. | more than 100 sera from patients with scleroderma crest (calcinosis, raynaud phenomenon, esophageal dismotility, sclerodactyly, telangiectasia) were tested in order to detect antigenic nuclear components of the field bean vicia faba (2n = 12). kinetochores of mitotic chromosomes and prekinetochores of interphase cells from root-tip meristems were specifically labelled via an indirect immunofluorescence procedure by antibodies of one of these sera. in 44% of interphase nuclei in which centromeres ... | 1995 | 7704412 |
| phaseolus vulgaris-leucoagglutinin tracing of commissural fibers to the rat dentate gyrus: evidence for a previously unknown commissural projection to the outer molecular layer. | numerous studies have shown a lamina-specific termination of commissural fibers to the dentate gyrus in the inner molecular layer. however, the exact course and arborization pattern of individual fibers remained unknown. in this study, the commissural fiber tract to the dentate gyrus of the rat has been studied using the anterograde tracer phaseolus vulgaris-leucoagglutinin (pha-l), which labels individual axons and their collaterals. following iontophoretic application of the tracer, anterograd ... | 1995 | 7714239 |
| characterization of glycoconjugate expression during development of meckel's cartilage in the rat. | the staining patterns of 24 biotinylated lectins were analyzed in serial sections of the mandible of 13- to 21-day-old rat embryos by means of the avidin-biotin-peroxidase method. a ubiquitous distribution of binding sites was demonstrated after incubation with con a (canavalia ensiformis), dsl (datura stramonium; except bone matrix), and wga (triticum vulgare). ecl (erythrina cristagalli), gsl i (griffonia simplicifolia), sja (saphora japonica), vvl (vicia villosa), dba (dolichus biflorus), uea ... | 1995 | 7717533 |
| the efferent projections of the periaqueductal gray in the rat: a phaseolus vulgaris-leucoagglutinin study. i. ascending projections. | this study has examined the ascending projections of the periaqueductal gray in the rat. injections of phaseolus vulgaris-leucoagglutinin were placed in the dorsolateral or ventrolateral subregions, at rostral or caudal sites. from either region, fibers ascended via two bundles. the periventricular bundle ascended in the periaqueductal and periventricular gray matter. at the posterior commissure level, this bundle divided into a dorsal component that terminated in the intralaminar and midline th ... | 1995 | 7721984 |
| the efferent projections of the periaqueductal gray in the rat: a phaseolus vulgaris-leucoagglutinin study. ii. descending projections. | the descending projections of the periaqueductal gray (pag) have been studied in the rat using the anterograde tracer phaseolus vulgaris-leucoagglutinin. the tracer was injected into the dorsolateral or ventrolateral subdivisions of the pag at rostral or caudal sites. it was found that the patterns of the descending projections of the rostral and caudal parts of the dorsolateral pag were the same and that the patterns of the descending projections of the rostral and caudal parts of the ventrolat ... | 1995 | 7721985 |
| synaptic distribution of afferents from reticular nucleus in ventroposterior nucleus of cat thalamus. | this study was aimed at determining the synaptic circuitry that contributes to the alterations in thalamic function that accompany changes in behavioral states. the somatosensory sector of the thalamic reticular nucleus (rtn) was identified by microelectrode recording in cats and injected with phaseolus vulgaris-leucoagglutinin (pha-l). the axons of labeled rtn cells gave rise to collaterals within the rtn and continued into the dorsal thalamus where they terminated predominately in the ventral ... | 1995 | 7721989 |
| differences in molecular biological, biological and growth characteristics between the immortal and malignant hamster pancreatic cells. | we compared morphological, biological and molecular biological patterns of a newly established, spontaneously immortalized pancreatic ductal cell line, taka-1, with a hamster pancreatic ductal adenocarcinoma cell line, pc-1. pc-1 cells grew in a monolayer on plastic tissue culture flasks, whereas taka-1 cells required type i collagen gel matrix to propagate. the growth rate and argyrophilic nuclear organizer region (ag-nor) counts were greater in pc-1 cells than in taka-1 cells. more taka-1 cell ... | 1995 | 7728976 |
| specific expression in reproductive tissues and fate of a mitochondrial sterility-associated protein in cytoplasmic male-sterile bean. | in common bean, cytoplasmic male sterility has been associated with a unique sequence found in the mitochondrial genome, designated pvs (for phaseolus vulgaris sterility sequence). within the pvs sequence, two open reading frames are encoded, orf98 and orf239. we have raised rabbit polyclonal antibodies against pvs-orf239 to evaluate the role of this putative male sterility-associated protein. histological investigation of pollen development revealed that in the male-sterile bean line, callose d ... | 1995 | 7734962 |
| changes in glycan distribution within the porcine interhaemal barrier during gestation. | changing patterns of glycan distribution are described in porcine placentae at 15, 19, 26, 43, 58, 69 and 109 days gestation, using a carefully selected panel of lectins that allowed partial analysis of saccharide classes and sequences. the lectins used were from galanthus nivalis, pisum sativum, phaseolus vulgaris (leukohaemagglutinin), triticum vulgaris, tetragonolobus purpureus, ulex europaeus-1, arachis hypogaea, erythrina cristagalli, glycine max, maclura pomifera, wisteria floribunda, doli ... | 1995 | 7736552 |
| the effect of l-tryptophane on yield of field bean and activity of soil microorganisms. | the pot trial was performed to study the effect of l-tryptophane (as an auxin precursor) applied in the amount 0.3 and 3.0 mg per 1 kg of the soil on yield and the chemical composition of field bean. the effects of this compound on dehydrogenases activity in the cells rhizobium leguminosarum isolated from root nodules, soil dehydrogenases activity and number of microorganisms from different systematic or physiological groups were also studied. the effects of l-tryptophane were compared to indole ... | 1994 | 7740988 |
| projections from the central cervical nucleus to the cerebellar nuclei in the rat, studied by anterograde axonal tracing. | projections from the central cervical nucleus (ccn) to the cerebellar nuclei were examined following injections of phaseolus vulgaris-leucoagglutinin or cholera toxin subunit b into the c1-c3 segments in the rat. labeled axons and terminals were immunohistochemically demonstrated. labeled spinocerebellar fibers arising from the ccn entered the cerebellum through the inferior and the superior cerebellar peduncles. labeled mossy fiber terminals were seen in lobules i-vi, sublobule viib, lobules vi ... | 1995 | 7745133 |
| re-examination of the plasticity of the corticothalamic projection after unilateral neonatal lesion of the sensorimotor cortex in the rat: a phaseolus vulgaris-leucoagglutinin tracing study. | after unilateral lesion of the sensorimotor cortex in neonatal rats (postnatal days 2-4), the reorganization of the corticothalamic projection originating from the opposite intact sensorimotor cortex was studied. three months after the lesion, the anterograde tracer phaseolus vulgaris-leucoagglutinin (pha-l) was injected in the caudal forelimb area (cfa) of the intact opposite sensorimotor cortex to label the corticothalamic axons, in order to establish their trajectory as well as the distributi ... | 1995 | 7751603 |
| afferent projections to the rat locus coeruleus demonstrated by retrograde and anterograde tracing with cholera-toxin b subunit and phaseolus vulgaris leucoagglutinin. | the aim of this study was to examine the afferents to the rat locus coeruleus by means of retrograde and anterograde tracing experiments using cholera-toxin b subunit and phaseolus leucoagglutinin. to obtain reliable injections of cholera-toxin b in the locus coeruleus, electrophysiological recordings were made through glass micropipettes containing the tracer and the noradrenergic neurons of the locus coeruleus were identified by their characteristic discharge properties. after iontophoretic in ... | 1995 | 7753394 |
| neuropeptide ff-containing efferent projections from the medial hypothalamus of rat: a phaseolus vulgaris leucoagglutinin study. | neuropeptide ff (fmrfamide-like peptide, morphine-modulating peptide) is an octapeptide isolated from the bovine brain. there is evidence that neuropeptide ff participates in the modulation of nociceptive mechanisms. neuropeptide ff acts through its own receptors which are distinct from the opiate receptors. in the rat brain neuropeptide ff is found in two major cell populations. we have studied the efferent connections of the hypothalamic neuropeptide ff-containing cell group, which is located ... | 1995 | 7753395 |
| direct projections from the anterior pretectal nucleus to the ventral medulla oblongata in rats. | the anterior pretectal nucleus has recently been implicated in the descending modulation of nociception. electrical stimulation of the nucleus was found to reduce the nociceptive responses of deep dorsal horn neurons and to inhibit spinally integrated withdrawal reflexes. it is believed that at least part of the descending inhibitory effects of the anterior pretectal nucleus are mediated by reticulospinal cells of the ventrolateral medulla. the purpose of the present study was to trace the direc ... | 1995 | 7753399 |
| the occurrence of ochratoxin a in coffee. | ochratoxin a (oa) is a nephrotoxic and nephrocarcinogenic mycotoxin which is predominantly produced by the two ubiquitous fungal genera, aspergillus and penicillium. oa is found in foodstuffs, predominantly in cereals but also in coffee beans. inconsistent results have been published regarding the influence of roasting on the oa content in roasted beans and the transfer into the coffee brew. in the present study an hplc method was used for the detection of oa in green and roasted coffee beans as ... | 1995 | 7759018 |
| organization of the efferent projections from the spinal cervical enlargement to the parabrachial area and periaqueductal gray: a pha-l study in the rat. | the organization of efferent projections from the spinal cervical enlargement to the parabrachial (pb) area and the periaqueductal gray (pag) was studied in the rat by using microinjections of phaseolus vulgaris-leucoagglutinin (pha-l) into different laminae around the c7 level. the results demonstrated two areas of cervical enlargement which project in different ways to the pb area and pag. first, the superficial laminae (i, ii) showed a very dense projection, with a clear contralateral dominan ... | 1995 | 7759612 |
| topographic organization of spinal and trigeminal somatosensory pathways to the rat parabrachial and kölliker-fuse nuclei. | we examined the organization of somatosensory projections to the parabrachial (pb) and kölliker-fuse (kf) nuclei by employing the retrograde and anterograde axonal transport of fluorogold and phaseolus vulgaris-leucoagglutinin (pha-l), respectively. small pha-l injections were made into different parts of the spinal trigeminal complex, including the paratrigeminal nucleus, and into different segments and laminae of the spinal dorsal horn. the subnuclear distribution of axonal labeling in the pb ... | 1995 | 7759613 |
| topographical organization of the rat suprachiasmatic-paraventricular projection. | the suprachiasmatic nucleus (scn) is a dominant pacemaker involved in the generation of circadian rhythms in mammals. surprisingly, the expression of the many rhythms appears to be mediated via a limited efferent projection system of the pacemaker, of which the largest pathway terminates in the subparaventricular area and in the paraventricular nucleus of the hypothalamus. in order to investigate a possible topographical organization of this major outflow pathway of the scn, microiontophoretic i ... | 1995 | 7759617 |
| purification and molecular characterization of a soybean polygalacturonase-inhibiting protein. | a polygalacturonase-inhibiting protein (pgip) was detected in soybean (glycine max (l.) merr.) seedlings. the protein was purified from germinating seeds and appeared to consist of at least three components with very close molecular weights (between 37 and 40 kda) but each showing a unique n-terminal sequence. primers specific for n-terminal and c-terminal nucleotide sequences of field bean (phaseolus vulgaris l.) pgip were used in a polymerase chain reaction (pcr) on soybean dna, and only one a ... | 1994 | 7765794 |
| flow cytometric detection of bicarbonate-induced changes in lectin binding in boar and ram sperm populations. | boar and ram spermatozoa were incubated in tyrode's medium in the presence or absence of bicarbonate/co2, a component believed essential for capacitation. at intervals, samples were stained with a range of fitc-lectins to detect changes in surface glycoconjugates, using a rapid staining technique to avoid problems of lectin toxicity. the samples were then analysed directly by flow cytometry, using propidium iodide to distinguish dead cells. in the presence of bicarbonate, a live subpopulation of ... | 1995 | 7766409 |
| the nods gene of rhizobium tropici strain ciat899 is necessary for nodulation on phaseolus vulgaris and on leucaena leucocephala. | rhizobium tropici strain ciat899 induces nitrogen-fixing nodules on the roots of a wide range of tropical legumes, including phaseolus vulgaris and leucaena leucocephala. previously, a dna region of the ciat899 psym plasmid containing the common nodulation genes nodabc and one of the nodd alleles was characterized (p. van rhijn, b. feys, c. verreth, and j. vanderleyden, j. bacteriol. 175: 438-447, 1993). as reported here, the region immediately downstream of nodc contains the nodsu genes. the nu ... | 1995 | 7772799 |
| location of the active site of the bean alpha-amylase inhibitor and involvement of a trp, arg, tyr triad. | seeds of the common bean contain three homologous proteins: phytohaemagglutinin e, phytohaemagglutinin l and the lectin-like protein alpha-amylase inhibitor (alpha ai). whereas the active site of lectins has been studied in great detail, there is no information on the active site of the related protein alpha ai, which exerts its biological activity by making a 1:1 complex with alpha-amylase. alpha-amylase inhibitor is synthesized as a 30 kda precursor glycoprotein that needs to be processed at a ... | 1995 | 7772866 |
| red kidney beans--to eat or not to eat? | the importation of dry red kidney beans (a variety of the species phaseolus vulgaris) for cultivation or consumption in south africa is prohibited because of their potential toxicity to humans. it has been established that the haemagglutinating lectins (e.g. phytohaemagglutinin (pha)) in kidney beans are responsible for this toxicity. dry bean varieties available on the south african market for human consumption as well as locally produced (for this study) and imported dry red kidney beans and i ... | 1995 | 7777998 |
| inhibition of starch digestion by alpha-amylase inhibitor reduces the efficiency of utilization of dietary proteins and lipids and retards the growth of rats. | digestion/absorption and nutritional utilization of starch, protein and lipids were studied in rats fed diets containing purified kidney bean alpha-amylase inhibitor at levels of 0, 1.6, 3.3 and 6.6 g/kg diet. at the two higher levels, the growth rate of rats and the apparent digestibilities and utilization of dietary starch and protein were significantly less than in control rats, and losses of nitrogen, lipids and carbohydrate resulted in a significant reduction in dry body weight. some organs ... | 1995 | 7782910 |
| purification, characterization, and cdna cloning of profilin from phaseolus vulgaris. | profilin from common bean (phaseolus vulgaris l.) was purified to homogeneity by poly-l-pro affinity chromatography and gel filtration. the hypocotyl and symbiotic root nodule protein was detected as a single isoform with a 14.4-kd molecular mass and an isoelectric point of 5.3. partial amino acid and dna sequencing of a full-length cdna clone confirmed its identity as profilin. an antibody generated against the purified protein binds to a protein with the same molecular mass in leaves and nodul ... | 1995 | 7784501 |
| contact-dependent stimulation of monocytic cells and neutrophils by stimulated human t-cell clones. | by means of direct cell-cell contact, fixed, stimulated t lymphocytes trigger the production of interleukin-1 beta (il-1 beta) and tumour necrosis factor by monocytes and prime polymorphonuclear leucocytes (pmn) for the respiratory burst induced by n-formyl-methionyl-leucyl-phenylalanine (fmlp). in order to assess whether this activity is displayed by a particular subpopulation of t lymphocytes, 88 t-cell clones (tcc) were generated from healthy human peripheral blood. the clones were stimulated ... | 1995 | 7790031 |
| galactose-containing glycoconjugates of the iris, the aqueous outflow passages and the cornea in capsular glaucoma. a lectin histochemical study. | the aim of the study was to compare galactose-containing glycoconjugates of the iris, the aqueous outflow passages and the cornea with exfoliation material in capsular glaucoma. | 1995 | 7797082 |
| evolutionary relationships among proteins in the phytohemagglutinin-arcelin-alpha-amylase inhibitor family of the common bean and its relatives. | the common bean, phaseolus vulgaris, contains a family of defense proteins that comprises phytohemagglutinin (pha), arcelin, and alpha-amylase inhibitor (alpha ai). here we report eight new derived amino acid sequences of genes in this family obtained with either the polymerase chain reaction using genomic dna, or by screening cdna libraries made with rna from developing beans. these new sequences are: two alpha ai sequences and arcelin-4 obtained from a wild accession of p. vulgaris that is res ... | 1994 | 7811969 |
| organization of projections from the anterior hypothalamic nucleus: a phaseolus vulgaris-leucoagglutinin study in the rat. | anterior hypothalamic nucleus (ahn) projections were examined with the phaseolus vulgaris-leucoagglutinin (pha-l) method in adult male rats. labeled axons from the ahn follow three major routes. 1) a large ascending pathway ends densely in the telencephalon, particularly in the lateral septal nucleus. axons along this route provide moderate to dense input to the medial and lateral preoptic areas, and a few are also observed in the septofimbrial nucleus and fimbria; the latter end in the temporal ... | 1994 | 7814679 |
| organization of projections from the ventromedial nucleus of the hypothalamus: a phaseolus vulgaris-leucoagglutinin study in the rat. | the organization of projections from the four parts of the ventromedial nucleus (vmh) and a ventrolaterally adjacent region tentatively identified as the tuberal nucleus (tu) have been analyzed with small injections of the anterograde axonal tracer phaseolus vulgaris-leucoagglutinin (pha-l). extrinsic and intranuclear projections of each part of the vmh display clear quantitative differences, whereas the overall patterns of outputs are qualitatively similar. overall, the vmh establishes massive ... | 1994 | 7814684 |