| identification and characterization of a sucrose transporter isolated from the developing cotyledons of soybean. | reduced carbon produced in mature leaves is distributed throughout plants in the form of sucrose. sucrose transporter proteins (sut) play a crucial role in transporting sucrose. we isolated a cdna encoding a sucrose transporter, gmsut1, which is expressed in the developing cotyledons of soybean (glycine max). [14c]sucrose uptake assays demonstrate that gmsut1 has a k(m) of 5.6mm and a v(max) of 5.8 nmol sucrose min(-1)(mg cells)(-1), which are similar to those of the low-affinity-high-capacity s ... | 2003 | 12504891 |
| citeromyces siamensis sp. nov., a novel halotolerant yeast isolated in thailand. | two halotolerant yeast strains, h130(t) and h149, were isolated from dry salted squid and fermented soybeans, respectively, in thailand. both isolates grew by multilateral budding, produced asci that had one roughened spherical ascospore and contained ubiquinone q-8. these characteristics were shared by citeromyces matritensis, the only species of the genus citeromyces. strains h130(t) and h149 were differentiated from c matritensis by their ability to assimilate l-sorbose and l-lysine and to gr ... | 2002 | 12508903 |
| bacteria associated with cysts of the soybean cyst nematode (heterodera glycines). | the soybean cyst nematode (scn), heterodera glycines, causes economically significant damage to soybeans (glycine max) in many parts of the world. the cysts of this nematode can remain quiescent in soils for many years as a reservoir of infection for future crops. to investigate bacterial communities associated with scn cysts, cysts were obtained from eight scn-infested farms in southern ontario, canada, and analyzed by culture-dependent and -independent means. confocal laser scanning microscopy ... | 2003 | 12514048 |
| genistein activates p38 mitogen-activated protein kinase, inactivates erk1/erk2 and decreases cdc25c expression in immortalized human mammary epithelial cells. | genistein (4',5,7-trihydroxyisoflavone) is an isoflavonoid present in soybeans that exhibits anticarcinogenic effects in breast, colon and prostate cancer cells. we recently reported that genistein treatment of the immortalized but nonmalignant human mammary epithelial cell line mcf-10f resulted in growth arrest of mcf-10f cells in the g2 phase of the cell cycle, a large induction of the tyr15 phosphorylation of cdc2 (along with decreased activity of cdc2), increased expression of p21(waf/cip1) ... | 2003 | 12514295 |
| a host-specific bacteria-to-plant signal molecule (nod factor) enhances germination and early growth of diverse crop plants. | lipo-chitooligosaccharides (lcos), or nod factors, are host-specific bacteria-to-plant signal molecules essential for the establishment of a successful n(2)-fixing legume-rhizobia symbiosis. at submicromolar concentrations nod factors induce physiological changes in host and non-host plants. here we show that the nod factor nod bj v(c18:1,mefuc) of bradyrhizobium japonicum 532c enhances germination of a variety of economically important plants belonging to diverse botanical families: zea mays, o ... | 2003 | 12520335 |
| characterization of ndvd, the third gene involved in the synthesis of cyclic beta-(1 --> 3),(1 --> 6)-d-glucans in bradyrhizobium japonicum. | previously, we identified two genes in bradyrhizobium japonicum (ndvb, ndvc) that are required for cyclic beta-(1 --> 3),(1 --> 6)-d-glucan synthesis and successful symbiotic interaction with soybean (glycine max). in this study, we report a new open reading frame (orf1) located in the intergenic region between ndvb and ndvc, which is essential for beta-glucan synthesis and effective nodulation of g. max. this new gene is designated ndvd (nodule development). the ndvd translation product has a p ... | 2002 | 12556128 |
| soy foods and soybean isoflavones and menopausal health. | soybeans are a natural dietary source of isoflavones, which have estrogen-like properties. therefore, it is worthwhile to consider the implications for soy of the recently published findings of the heart and estrogen/progestin replacement study (hers) i/ii and the women's health initiative (whi). the whi found coronary heart disease (chd) risk to be increased in women receiving hormone replacement therapy, and both studies found increases in venous thromboembolic disease in such women. additiona ... | 2002 | 12557810 |
| pharmacological activities of genistein, an isoflavone from soy (glycine max): part i--anti-cancer activity. | phytoestrogens represent a family of plant compounds such as isoflavones, flavones and lignans. a variety of these plant compounds have been identified in various human body fluids. a wide range of commonly consumed foods contains appreciable amounts of these different phytoestrogens, viz. soy products are particularly good sources of isoflavones and lignans. accumulating evidences from molecular and cellular biology experiments, animal studies, and to a limited extent, human clinical trials sug ... | 2001 | 12562011 |
| pharmacological activities of genistein, an isoflavone from soy (glycine max): part ii--anti-cholesterol activity, effects on osteoporosis & menopausal symptoms. | phytoestrogens represent a family of plant compounds such as isoflavones, flavones and lignans. a wide range of commonly consumed foods contains appreciable amounts of different phytoestrogens such as isoflavones and lignans. soy and its products are particularly good sources of isoflavones and lignans. the evidence reviewed here represents the beneficial effects of most potential and promising isoflavone, genistein in various types of diseases such as osteoporosis, cardiovascular diseases, meno ... | 2001 | 12562012 |
| cyanidin 3-o-beta-d-glucoside isolated from skin of black glycine max and other anthocyanins isolated from skin of red grape induce apoptosis in human lymphoid leukemia molt 4b cells. | cyanidin 3-o-beta-d-glucoside (cg) was purified from black bean seed coat and other anthocyanins were prepared from red grape skin. these anthocyanins were identified by mass, and 1h- and 13c-nmr. the effects of four anthocyanins on cell viability in human leukemia molt 4b cells were investigated. the anthocyanins displayed strong growth inhibitory effects against human leukemia molt 4b cells. morphological changes showing apoptotic bodies were observed in the molt 4b cells treated with these an ... | 2003 | 12579261 |
| a physical map of a gene-dense region in soybean linkage group a2 near the black seed coat and rhg (4) loci. | soybean ( glycine max l. merrill) linkage group a2 contains a major resistance gene to the soybean cyst nematode ( heterodera glycines ichinohe) at the rhg (4) locus near a gene encoding aspartokinase homoserine dehydrogenase (ak-hsdh) and also near the i locus affecting seed coat color. to identify clones related to this region of the genome, we used a pcr assay using primers designed from a gene encoding ak-hsdh to screen approximately 40,000 clones from a bacterial artificial chromosome (bac) ... | 2002 | 12582695 |
| common loci underlie field resistance to soybean sudden death syndrome in forrest, pyramid, essex, and douglas. | soybean [ glycine max (l.) merr.] sudden death syndrome (sds) caused by fusarium solani f. sp. glycines results in severe yield losses. resistant cultivars offer the most-effective protection against yield losses but resistant cultivars such as 'forrest' and 'pyramid' vary in the nature of their response to sds. loci underlying sds resistance in 'essex' x forrest are well defined. our objectives were to identify and characterize loci and alleles that underlie field resistance to sd ... | 2002 | 12582700 |
| characterization of a plant homolog of hop, a cochaperone of hsp90. | the 90-kd molecular chaperone hsp90 is the key component of a multiprotein chaperone complex that facilitates folding, stabilization, and functional modulation of a number of signaling proteins. the components of the animal chaperone complex include hsp90, hsp70, hsp40, hop, and p23. the animal hop functions to link hsp90 and hsp70, and it can also inhibit the atpase activity of hsp90. we have demonstrated the presence of an hsp90 chaperone complex in plant cells, but not all components of the c ... | 2003 | 12586877 |
| effects of bowman-birk inhibitor on rat colon carcinogenesis. | the present study was undertaken to determine whether the bowman-birk inhibitor (bbi) could prevent colon carcinogenesis in rats treated with dimethylhydrazine (dmh) and whether there were adverse side effects associated with treatment with bbi for cancer prevention. bbi was evaluated in the forms of purified bbi (pbbi) or an extract of soybeans enriched in bbi, termed bbi concentrate (bbic). the results demonstrate that pbbi and bbic reduced the incidence and frequency of tumors in dmh-treated ... | 2002 | 12588698 |
| description of the soybean pod gall midge, asphondylia yushimai sp. n. (diptera: cecidomyiidae), a major pest of soybean and findings of host alternation. | the soybean pod gall midge is an important pest of soybean in japan and is known to occur also in indonesia and china. this gall midge is described from japan as asphondylia yushimai sp. n. and is clearly distinguished from its congeners by the arrangement of the lower frontal horns of the pupa and the sequence of the mtdna coi region. it is concluded that prunus zippeliana miquel is a winter host of the soybean pod gall midge since haplotypes of the soybean pod gall midge coincide with those of ... | 2003 | 12593685 |
| a preliminary study on the use of tempe-based formula as a weaning diet in nigeria. | tempe, an indonesian mold fermented food, was prepared from cowpeas and soybeans using the traditional oriental process with modifications where appropriate. four complementary foods were developed from whole maize meal or dehydrated fermented maize (ogi) flour fortified with either cowpea tempe or soybean tempe. wholesomeness and potentials of the vanilla-flavored foods as weaning diets were determined. the nutrient content of all the developed products were within the range prescribed by the f ... | 2002 | 12602942 |
| dietary supplementation with fermented soybeans suppresses intimal thickening. | although soy foods have been consumed for more than 1000 y, it is only in the past 20 y that they have made inroads into western diets. we investigated the effect of dietary supplementation with natto extracts produced from fermented soybeans on intimal thickening of arteries after vessel endothelial denudation. natto extracts include nattokinase, a potent fibrinolytic enzyme having four times greater fibrinolytic activity than plasmin. intimal thickening was induced in the femoral arteries by i ... | 2003 | 12620531 |
| efficient soybean transformation using hygromycin b selection in the cotyledonary-node method. | the efficiency of soybean [glycine max (l.) merrill] transformation was significantly increased from an average of 0.7% to 16.4% by combining strategies to enhance agrobacterium tumefaciens-mediated t-dna delivery into cotyledonary-node cells with the development of a rapid, efficient selection protocol based on hygromycin b. wounded cotyledonary-node explants were inoculated with a. tumefaciens carrying either a standard-binary or super-binary plasmid and co-cultivated in the presence of mixtur ... | 2003 | 12624759 |
| antimutagenic effects of doenjang (korean fermented soypaste) and its active compounds. | doenjang (korean fermented soypaste) is one of the important fermented foods of korea. doenjang has been traditionally manufactured from meju, which is fermented rectangular shape molded from crushed cooked soybeans. the main microorganisms involved for meju fermentation are bacillus subtilis and molds such as rizopus sp., mucor sp., and aspergillus sp. we have already reported that doenjang is safe from mycotoxin, especially, aflatoxin contamination due to the manufacturing process of the doenj ... | 2003 | 12628502 |
| protective effect of soybean saponins and major antioxidants against aflatoxin b1-induced mutagenicity and dna-adduct formation. | saponins from various plant sources have been suggested as possible anticarcinogens. major dietary sources of saponins include legumes such as soybeans. this study was performed to determine the effect of soybean saponins on aflatoxin b(1)(afb(1))-induced mutagenicity and afb(1)-dna adduct formation using salmonella typhimurium and human liver hepatoma (hepg2) cells, respectively. major antioxidants including l-ascorbic acid, alpha-tocopherol, all-trans-retinol, and butylated hydroxytoluene (bht ... | 2002 | 12639399 |
| inheritance and allelism of resistance to soybean mosaic virus in zao18 soybean from china. | soybean mosaic disease caused by soybean mosaic virus (smv) occurs wherever soybean [glycine max (l.) merr.] is grown and is considered one of the most important soybean diseases in many areas of the world. use of soybean cultivars with resistance to smv is a very effective way of controlling the disease. china has rich soybean germplasm, but there is very limited information on genetics of smv resistance in chinese soybean germplasm and reaction of the resistance genes to smv strains g1-g7. the ... | 2002 | 12642647 |
| expression of an expansin gene is correlated with root elongation in soybean. | expansin is a family of proteins that catalyze long-term expansion of cell walls and has been considered a principal protein that affects cell expansion in plants. we have identified the first root-specific expansin gene in soybean (glycine max), gmexp1, which may be responsible for root elongation. expression levels of gmexp1 were very high in the roots of 1- to 5-d-old seedlings, in which rapid root elongation takes place. furthermore, gmexp1 mrna was most abundant in the root tip region, wher ... | 2003 | 12644651 |
| photosynthetic responses of soybean to soybean aphid (homoptera: aphididae) injury. | the soybean aphid, aphis glycines matsumara, was discovered in the united states in the summer of 2000. since that initial discovery, the aphid has spread across northern soybean production regions. in 2001, we examined the physiological responses of soybeans to low aphids densities (fewer than 50 aphids/leaf). in this study, we determined photosynthetic rates, leaf fluorescence responses, and photosynthetic responses to variable carbon dioxide and light levels. in addition, analyses for chlorop ... | 2003 | 12650361 |
| progression of alachlor-induced olfactory mucosal tumours. | alachlor is an herbicide used primarily in the production of corn (maize), peanuts, and soybeans and is associated with cancer of the nasal cavity, thyroid, and stomach in rats. previous work from our laboratory demonstrated that the nasal cavity tumours originate from the olfactory mucosa, and that neoplasms were present following 6 months of exposure (126 mg/kg/day in the diet). the studies presented herein were conducted to determine more precisely the earliest time point at which alachlor-in ... | 2002 | 12657139 |
| the 5-enolpyruvylshikimate-3-phosphate synthase of glyphosate-tolerant soybean expressed in escherichia coli shows no severe allergenicity. | the recombinant gene was amplified from the chromosomal dna of genetically-modified (gm) soybeans and identified as epsps encoding 5-enolpyruvylshikimate-3-phosphate synthase (epsps) which renders glyphosate resistance. the epsps structural gene was introduced in the pet28(a) plasmid for its expression in escherichia coli bl21(de3). it was confirmed that the maximal productivity of the epsps protein was achieved when cultivating the recombinant strain in a lb broth for 2 h after supplementing 1 ... | 2003 | 12661756 |
| evaluation of the estrogenic effects of legume extracts containing phytoestrogens. | seven legume extracts containing phytoestrogens were analyzed for estrogenic activity. methanol extracts were prepared from soybean (glycine max l.), green bean (phaseolus vulgaris l.), alfalfa sprout (medicago sativa l.), mung bean sprout (vigna radiata l.), kudzu root (pueraria lobata l.), and red clover blossom and red clover sprout (trifolium pratense l.). extracts of kudzu root and red clover blossom showed significant competitive binding to estrogen receptor beta (erbeta). estrogenic activ ... | 2003 | 12670155 |
| utilization of different soy products as affected by age in chicks. | two experiments were conducted to evaluate the utilization of several different soy products at different ages in new hampshire x columbian male chicks. six pens of eight chicks were fed dextrose-protein source diets (23% cp) containing 1 of 10 different protein sources from 0 to 21 d of age. excreta were collected at 0 to 2, 3 to 4, 7, 14, and 21 d of age, and amen and amino acid (aa) digestibility were determined using acid-insoluble ash as a marker. protein sources evaluated were as follows: ... | 2003 | 12705407 |
| comparison of hormonal activity (estrogen, androgen and progestin) of standardized plant extracts for large scale use in hormone replacement therapy. | extracts from red clover (trifolium pratense), soybean (glycine max.) and black cohosh (cimicifuga racemosa) are frequently used as alternative compounds for hormone replacement therapy (hrt) to treat menopausal disorders. fifteen commercially available products made either from red clover, soybean or black cohosh were tested in in vitro assays in this study. the main polycyclic phenolic compounds of soy and red clover products were biochanin a, genistein, daidzein, formononetin, and glycitein. ... | 2003 | 12711012 |
| two critical factors are required for efficient transformation of multiple soybean cultivars: agrobacterium strain and orientation of immature cotyledonary explant. | an efficient transformation system was developed for multiple soybean [glycine max (l.) merrill.] cultivars using agrobacterium-mediated gene transfer. a significantly high number of hygromycin-resistant somatic embryos (ses) was obtained when immature zygotic cotyledons were inoculated with agrobacterium tumefaciens strain kyrt1 and when the abaxial side of explants was oriented upwards (i.e., the adaxial side of explants was in contact with the medium). most hygromycin-resistant ses on selecti ... | 2003 | 12721637 |
| rpon (sigma(54)) is required for plasmid-encoded coronatine biosynthesis in pseudomonas syringae. | the plant pathogen pseudomonas syringae pv. glycinea pg4180 produces coronatine (cor), a phytotoxin which functions as a virulence factor in bacterial blight of soybeans. the cor biosynthetic gene cluster in pg4180 is borne on a 90-kb plasmid named p4180a. although pathway-specific regulatory genes for cor have been identified, global regulatory genes for cor production in pg4180 remain undefined. in the present study, we evaluated the role of rpon, which encodes sigma(54), in the virulence of s ... | 2003 | 12726764 |
| concentrations of choline-containing compounds and betaine in common foods. | choline is important for normal membrane function, acetylcholine synthesis and methyl group metabolism; the choline requirement for humans is 550 mg/d for men (adequate intake). betaine, a choline derivative, is important because of its role in the donation of methyl groups to homocysteine to form methionine. in tissues and foods, there are multiple choline compounds that contribute to total choline concentration (choline, glycerophosphocholine, phosphocholine, phosphatidylcholine and sphingomye ... | 2003 | 12730414 |
| isoflavones and skeletal health: are these molecules ready for clinical application? | a review of the recent literature on the effects of isoflavones was undertaken to determine whether molecules such as genistein and daidzein, aglycone derivatives of soybeans, might have benefit in the prevention and treatment of osteoporosis. the current standard for science-based medicine is the documentation of efficacy of an agent under controlled, randomized, prospective conditions. a few short clinical investigations have been undertaken using isoflavones (along with soy protein), but they ... | 2003 | 12730743 |
| characterization of poly-gamma-glutamate hydrolase encoded by a bacteriophage genome: possible role in phage infection of bacillus subtilis encapsulated with poly-gamma-glutamate. | some bacillus subtilis strains, including natto (fermented soybeans) starter strains, produce a capsular polypeptide of glutamate with a gamma-linkage, called poly-gamma-glutamate (gamma-pga). we identified and purified a monomeric 25-kda degradation enzyme for gamma-pga (designated gamma-pga hydrolase, pghp) from bacteriophage phinit1 in b. subtilis host cells. the monomeric pghp internally hydrolyzed gamma-pga to oligopeptides, which were then specifically converted to tri-, tetra-, and penta- ... | 2003 | 12732513 |
| initial crop growth in soil collected from a closed animal waste lagoon. | in the 21st century, remediation of the soil beneath animal waste lagoons will become an important issue, as they are closed due to environmental regulations or to abandonment. the possibility of growing crops in the soil, which has high concentrations of ammonium-n, has not been studied. the objective of this experiment was to determine if crop species would germinate and grow in lagoon soil. soil was gathered from a lagoon that had received wastes from swine (sus scrofa) and beef (bos taurus) ... | 2003 | 12733569 |
| estimates of conserved microsynteny among the genomes of glycine max, medicago truncatula and arabidopsis thaliana. | a growing body of research indicates that microsynteny is common among dicot genomes. however, most studies focus on just one or a few genomic regions, so the extent of microsynteny across entire genomes remains poorly characterized. to estimate the level of microsynteny between medicago truncatula (mt) and glycine max (soybean), and also among homoeologous segments of soybean, we used a hybridization strategy involving bacterial artificial chromosome (bac) contigs. a mt bac library consisting o ... | 2003 | 12748777 |
| biogenic peptides and their potential use. | this paper reviews bioactive peptides, biogenic peptides, opioid peptides, immunostimulating peptides, mineral soluble peptides, antihypertensive peptides and antimicrobial peptides originating from food materials and enzymatic hydrolysis of proteins. antihypertensive peptides are extensively reviewed and have been divided into angiotensin i-converting enzyme inhibitory peptides and others. these peptides are produced in the enzymatic hydrolysate of treated food materials such as milk, animal an ... | 2003 | 12769742 |
| variations in metabolism of the soy isoflavonoid daidzein by human intestinal microfloras from different individuals. | isoflavonoids found in legumes, such as soybeans, are converted by intestinal bacteria to metabolites that might have increased or decreased estrogenic activity. variation in the effects of dietary isoflavonoids among individuals has been attributed to differences in their metabolism by intestinal bacteria. to investigate this variation, the metabolism of the isoflavonoid daidzein by bacteria from ten fecal samples, provided at different times by six individuals on soy-containing diets, was comp ... | 2003 | 12783157 |
| enhancement of cold tolerance and inhibition of lipid peroxidation by citrus dehydrin in transgenic tobacco. | citrus ( citrus unshiu marcov.) dehydrin in response to chilling stress was overexpressed in tobacco ( nicotiana tabacum l.), and the cold stress tolerance of transgenics at low temperature was analyzed. the freezing at -4 degrees c for 3 h of 24 independent lines indicated that a phenotype expressing citrus dehydrin showed less electrolyte leakage than the control. dehydrin protein content was correlated with freezing tolerance in transgenics. dehydrin-expressing tobacco exhibited earlier germi ... | 2003 | 12783337 |
| [legume cross-reactivity]. | legumes are dicotyledonous plants belonging to the fabales order. the main distinctive characteristic of which is their fruit (legumen, seeds contained in pods). this botanical order is formed by three families: mimosaceae, caesalpiniaceae and papilionaceae or fabacea. the papilionaceae family includes the most important allergenic species: lens culinaris (lentil), cicer arietinum (chick-pea), pisum sátivum (pea), arachis hipogea (peanut), phaseolus vulgaris (bean) y glycine max (soy). legumes a ... | 2003 | 12783766 |
| citrate synthase mutants of sinorhizobium fredii usda257 form ineffective nodules with aberrant ultrastructure. | the tricarboxylic acid (tca) cycle plays an important role in generating the energy required by bacteroids to fix atmospheric nitrogen. citrate synthase is the first enzyme that controls the entry of carbon into the tca cycle. we cloned and determined the nucleotide sequence of the glta gene that encodes citrate synthase in sinorhizobium fredii usda257, a symbiont of soybeans (glycine max [l.] merr.) and several other legumes. the deduced citrate synthase protein has a molecular weight of 48,198 ... | 2003 | 12788763 |
| genetic diversity of fast-growing rhizobia that nodulate soybean ( glycine max l. merr). | the fast-growing rhizobium sp. strain ngr234, isolated from papua new guinea, and 13 strains of sinorhizobium fredii, isolated from china and vietnam, were fingerprinted by means of rapd, rep, eric and ardra. eric, rep and rapd markers revealed a considerable genetic diversity among fast-growing rhizobia. chinese isolates showed higher levels of diversity than those strains isolated from vietnam. ardra analysis revealed three different genotypes among fast-growing rhizobia that nodulate soybean, ... | 2003 | 12802480 |
| symbiotic gene activation is interrupted by endocrine disrupting chemicals. | endocrine disrupting chemicals (edcs) include organochlorine pesticides, plastics manufacturing by-products, and certain herbicides. these chemicals have been shown to disrupt hormonal signaling in exposed wildlife, lab animals, and mammalian cell culture by binding to estrogen receptors (er-+/- and er-) and affecting the expression of estrogen responsive genes. additionally, certain plant chemicals, termed phytoestrogens, are also able to bind to estrogen receptors and modulate gene expression, ... | 2001 | 12805768 |
| estimated historical and current nitrogen balances for illinois. | the midwest has large riverine exports of nitrogen (n), with the largest flux per unit area to the mississippi river system coming from iowa and illinois. we used historic and current data to estimate n inputs, outputs, and transformations for illinois where human activity (principally agriculture and associated landscape drainage) have had a dominant impact. presently, approximately 800,000 mg of n is added each year as fertilizer and another 420,000 mg is biologically fixed, primarily by soybe ... | 2001 | 12805881 |
| nitrogen use and carbon sequestered by corn rotations in the northern corn belt, u.s. | diversified crop rotation may improve production efficiency, reduce fertilizer nitrogen (n) requirements for corn (zea mays l.), and increase soil carbon (c) storage. objectives were to determine effect of rotation and fertilizer n on soil c sequestration and n use. an experiment was started in 1990 on a barnes clay loam (u.s. soil taxonomy: fine-loamy, mixed, superactive, frigid calcic hapludoll) near brookings, sd. tillage systems for corn-soybean ( glycine max [l.] merr.) rotations were conve ... | 2001 | 12806069 |
| genetic study of a lethal necrosis to soybean mosaic virus in pi 507389 soybean. | pi 507389 soybean [glycine max (l.) merr.], a large-seeded line from japan, exhibits a rapid, lethal, necrotic response to strains g1, g2, g5, and g6 of soybean mosaic virus (smv). unlike the hypersensitive necrotic reaction, this stem-tip necrosis can be a serious threat to soybean production. to investigate the genetic basis of lethal necrosis (ln), pi 507389 was crossed with the susceptible (s) cv. lee 68 and with resistant (r) lines pi 96983, cv. york, and cv. marshall, which carry single do ... | 2003 | 12816960 |
| determining whether transgenic and endogenous plant dna and transgenic protein are detectable in muscle from swine fed roundup ready soybean meal. | questions regarding the digestive fate of dna and protein from transgenic feed have been raised in regard to human consumption and commercial trade of animal products (e.g., meat, milk, and eggs) from farm animals fed transgenic crops. using highly sensitive, well-characterized analytical methods, pork loin samples were analyzed for the presence of fragments of transgenic and endogenous plant dna and transgenic protein from animals fed meal prepared from conventional or glyphosate-tolerant round ... | 2003 | 12817492 |
| dilinoleoylphosphatidylcholine reproduces the antiapoptotic actions of polyenylphosphatidylcholine against ethanol-induced hepatocyte apoptosis. | polyenylphosphatidylcholine (ppc), a mixture of polyunsaturated phosphatidylcholines extracted from soybeans, attenuates hepatocyte apoptosis induced by ethanol feeding of rats. our aims were to evaluate whether dilinoleoylphosphatidylcholine (dlpc), the main component of ppc, reproduces the antiapoptotic actions of ppc against alcohol-induced apoptosis and to identify the apoptotic proteins that are affected by ppc and dlpc. | 2003 | 12824822 |
| effects of heat treatment on the nutritional value of raw soybean selected for low trypsin inhibitor activity. | 1. two broiler experimentss and a layer experiments were conducted on kunitz trypsin inhibitor (kti) soybeans (sb) of low trypsin inhibitor (ti) activity to determine their nutritive value when included as mash in least-cost poultry diets. 2. experiment 1 compared chick performance on the kti or raw sb using a commercial full-fat sb meal (ffsbm) and a solvent extracted sb meal (sbm) as controls during a 20 d experimental period. broiler experiment 2 compared kti and raw sb, non-steamed, or steam ... | 2003 | 12828216 |
| the production of a new tempeh-like fermented soybean containing a high level of gamma-aminobutyric acid by anaerobic incubation with rhizopus. | a cultivation procedure for the preparation of a new tempeh-like fermented soybean containing a high level of gamma-aminobutyric acid was developed. steamed soybeans were incubated aerobically with rhizopus microsporus var. oligosporus ifo 8631 for 20 h, and then anaerobically incubated for 5 h by replacement of the atmosphere with nitrogen. the gaba content in the aerobically fermented soybeans was about 30 mg per 100 g dry fermented soybeans, while the anaerobically cultivation was about 370 m ... | 2003 | 12834278 |
| adaptation of cucumber mosaic virus soybean strains (ssvs) to cultivated and wild soybeans. | cucumber mosaic virus soybean strains formerly called soybean stunt virus (ssv) were inoculated onto 23 wild soybeans collected from four asian countries to investigate their infectivity in order to improve understanding of the co-evolution of ssvs and soybean. ssv inoculation resulted in systemic infection in most of the wild soybeans used. however, an ssv strain (ssv-in), which was isolated in indonesia, did not result in systemic infection of many of the wild soybeans distributed in southern ... | 2003 | 12835932 |
| determination of enzymatic activity of 5-enolpyruvylshikimate-3-phosphate synthase by lc/ms. | a liquid chromatography-mass spectrometry (lc/ms) method for determining the enzymatic activity of 5-enolpyruvylshikimate-3-phosphate synthase (epsp synthase), an enzyme of the shikimate pathway, was developed. epsp synthase catalyzes the formation of 5-enolpyruvylshikimate-3-phosphate (epsp) from shikimate-3-phosphate (s-3-p) and phosphoenolpyruvate (pep) in microorganisms and plants. the enzymatic activity of epsp synthase was assessed by the determination of epsp after a 30-min incubation wit ... | 2003 | 12846153 |
| extracellular proteins involved in soybean cultivar-specific nodulation are associated with pilus-like surface appendages and exported by a type iii protein secretion system in sinorhizobium fredii usda257. | several gram-negative plant and animal pathogenic bacteria have evolved a type iii secretion system (ttss) to deliver effector proteins directly into the host cell cytosol. sinorhizobium fredii usda257, a symbiont of soybean and many other legumes, secretes proteins called nops (nodulation outer proteins) into the extracellular environment upon flavonoid induction. mutation analysis and the nucleotide sequence of a 31.2-kb symbiosis (sym) plasmid dna region of usda257 revealed the existence of a ... | 2003 | 12848427 |
| a purl mutant of sinorhizobium fredii hh103 is symbiotically defective and altered in its lipopolysaccharide. | the pleiotropic phenotype of an auxotrophic purl mutant (svq295) of sinorhizobium fredii hh103 has been investigated. svq295 forms colonies that are translucent, produce more slime and absorb less congo red than those of wild-type strain hh103. svq295 did not grow in minimal medium unless the culture was supplemented with thiamin and adenine or with thiamin and aica-riboside (5-aminoimidazole-4-carboxamide 1-beta-d-ribofuranoside), an intermediate of purine biosynthesis. bacterial cultures suppl ... | 2003 | 12855732 |
| triterpenoids from glycine max decrease invasiveness and induce caspase-mediated cell death in human snb19 glioma cells. | in recent years there has been an increasing interest in compounds present in foods that may prevent or slow the progression of chronic illnesses, such as cardiovascular disease, osteoporosis and cancer. saponins have been reported to have important time-dependent anti-cancer properties. we have used a highly purified and characterized saponin fraction containing the soyasapogenol b glycosides (the 'b group' saponins) from soybeans (glycine max l.) to demonstrate a reduction in snb 19 human glio ... | 2003 | 12856725 |
| [the necessity of a surplus in the nitrogen balance in the rumen of cows having high milk production]. | the amount of crude protein entering the small intestine (nxp) and the nitrogen balance in the rumen (rnb) are the two components of the german protein evaluation system. although there is no scientific evidence, feeding strategies for the practice imply an excess in rnb, particularly in diets for high-yielding cows. this study was undertaken to investigate whether cows benefit from an excess in rnb under conditions of sufficient nxp supply. three rations were used equal in energy (7.2 mj net en ... | 2003 | 12864908 |
| the soybean nramp homologue, gmdmt1, is a symbiotic divalent metal transporter capable of ferrous iron transport. | iron is an important nutrient in n2-fixing legume root nodules. iron supplied to the nodule is used by the plant for the synthesis of leghemoglobin, while in the bacteroid fraction, it is used as an essential cofactor for the bacterial n2-fixing enzyme, nitrogenase, and iron-containing proteins of the electron transport chain. the supply of iron to the bacteroids requires initial transport across the plant-derived peribacteroid membrane, which physically separates bacteroids from the infected pl ... | 2003 | 12887581 |
| effect of orally and intraperitoneally administered plant lectins on food consumption of rats. | a panel of orally administered lectins (100 mg/kg b.w.) of different binding specificities was tested for suppression of voluntary food consumption in prefasted rats. pha isolectins (phaseolus vulgaris) and rpa-i (robinia pseudoacacia) were found to exert a marked and significant effect, but two other gut-binding lectins, i.e. sba (glycine max) and wga (triticum vulgar) and several non-binding lectins were ineffective. in cannulated rats pha infused into the duodenum induced food suppression, i. ... | 2003 | 12903908 |
| effect of trehalose on survival of bradyrhizobium japonicum during desiccation. | a major reason for the ineffectiveness of legume inoculants in the field is the rapid death of rhizobia because of desiccation. the major purpose of this study was to identify conditions under which alpha,alpha-trehalose would improve survival of bradyrhizobium japonicum during desiccation. | 2003 | 12911696 |
| interallelic complementation at the ubiquitous urease coding locus of soybean. | soybean (glycine max [l.] merrill) mutant aj6 carries a single recessive lesion, aj6, that eliminates ubiquitous urease activity in leaves and callus while retaining normal embryo-specific urease activity. consistently, aj6/aj6 plants accumulated urea in leaves. in crosses of aj6/aj6 by urease mutants at the eu1, eu2, and eu3 loci, f(1) individuals exhibited wild-type leaf urease activity, and the f(2) segregated urease-negative individuals, demonstrating that aj6 is not an allele at these loci. ... | 2003 | 12913138 |
| the anticarcinogenic potential of soybean lectin and lunasin. | cancer is one of the leading causes of death worldwide, generally exceeded only by cardiovascular disease in the developed world. the number of people diagnosed with cancer within the next few decades is expected to double. there will therefore be increased demand for novel diagnostic and medical therapies that use new non-traditional sources. soybeans contain a variety of anticarcinogenic phytochemicals. recently, there has been increased interest in the potential health benefits of bioactive p ... | 2003 | 12918876 |
| quantification of the environmental impact of different dietary protein choices. | quantitative environmental evaluations of meat, fresh vegetables, and processed protein based on soybeans suggest that the environmental burden of vegetarian foods is usually relatively low when production and processing are considered. the environmental comparison of cheese varieties made from cow milk and directly from lupine and the evaluation of energy inputs in fish protein and vegetable protein also suggest an environmental advantage for vegetarian food. in the evaluation of processed prot ... | 2003 | 12936964 |
| immune response of postpartum dairy cows fed flaxseed. | thirty holstein cows were allotted at calving to 10 groups of three cows blocked for similar calving dates to determine the effects of dietary fatty acids on functional properties of immunocompetent cells in early lactation and at breeding. cows were assigned at calving to one of three isonitrogenous, isoenergetic, and isolipidic supplements based on either calcium salts of palm oil, megalac, micronized soybeans, or whole flaxseed. on the day of ai and 20 d later, cows were injected with ovalbum ... | 2003 | 12939089 |
| genistein enhances the cisplatin-induced inhibition of cell growth and apoptosis in human malignant melanoma cells. | genistein, a naturally occurring isoflavone found chiefly in soybeans, has been reported to be a potent antitumor agent. genistein is presumed to exert multiple effects related to the inhibition of cancer growth. metastatic melanoma is a chemotherapy-refractory neoplasm. the present study was designed to explore the possible activity of genistein to inhibit the aberrant proliferation and to induce apoptosis of human malignant melanoma cells in cooperation with cisplatin treatment. five human mel ... | 2003 | 12950722 |
| lipoxygenase activity in altered gravity. | lipoxygenases are a family of enzymes which dioxygenate unsaturated fatty acids, thus initiating lipoperoxidation of membranes or the synthesis of signalling molecules, or inducing structural and metabolic changes in the cell. this activity is the basis for the critical role of lipoxygenases in a number of pathophysiological conditions, both in animals and plants. we review the effects of microgravity on the catalytic efficiency of purified soybean (glycine max) lipoxygenase-1, as well as the mo ... | 2002 | 12951691 |
| host sanctions and the legume-rhizobium mutualism. | explaining mutualistic cooperation between species remains one of the greatest problems for evolutionary biology. why do symbionts provide costly services to a host, indirectly benefiting competitors sharing the same individual host? host monitoring of symbiont performance and the imposition of sanctions on 'cheats' could stabilize mutualism. here we show that soybeans penalize rhizobia that fail to fix n(2) inside their root nodules. we prevented a normally mutualistic rhizobium strain from coo ... | 2003 | 12955144 |
| effect of supplemental nutrient source on heifer growth and reproductive performance, and on utilization of corn silage-based diets by beef steers. | two experiments were conducted to determine effects of oilseeds or soybean hulls on growth and reproductive performance of heifers and utilization of corn silage diets by growing beef cattle. in exp. 1, 96 beef heifers (249 kg of bw) were used in a randomized complete block design. treatments were as follows: 1) corn and soybean meal (con) at 56% of the dmi; 2) whole linted cottonseed at 15% of the dmi (cot); 3) whole raw soybeans at 15% of the dmi (sb); or 4) pelleted soyhulls at 30% of the dmi ... | 2003 | 12968713 |
| does lowering glutamine synthetase activity in nodules modify nitrogen metabolism and growth of lotus japonicus? | a cdna encoding cytosolic glutamine synthetase (gs) from lotus japonicus was fused in the antisense orientation relative to the nodule-specific lbc3 promoter of soybean (glycine max) and introduced into l. japonicus via transformation with agrobacterium tumefaciens. among the 12 independent transformed lines into which the construct was introduced, some of them showed diminished levels of gs1 mrna and lower levels of gs activity. three of these lines were selected and their t(1) progeny was furt ... | 2003 | 12970491 |
| genetic and physical localization of the soybean rpg1-b disease resistance gene reveals a complex locus containing several tightly linked families of nbs-lrr genes. | alleles or tightly linked genes at the soybean (glycine max l. merr.) rpg1 locus confer resistance to strains of pseudomonas syringae pv. glycinea that express the avirulence genes avrb or avrrpm1. we have previously mapped rpg1-b (the gene specific for avrb) to a cluster of resistance genes (r genes) with diverse specificities in molecular linkage group f. here, we describe the high-resolution physical and genetic mapping of rpg1-b to a 0.16-cm interval encompassed by two overlapping bac clones ... | 2003 | 12971605 |
| metabolism of the soybean isoflavone glycoside genistin in vitro by human gut bacteria and the effect of prebiotics. | the isoflavone genistein is found predominantly in soybeans and is thought to possess various potent biological properties, including anti-carcinogenic effects. studies have shown that genistein is extensively degraded by the human gut microflora, presumably with a loss of its anti-carcinogenic action. the aim of the present study was to investigate the potential of a prebiotic to divert bacterial metabolism away from genistein breakdown: this may be of benefit to the host. faecal samples were o ... | 2003 | 13129470 |
| high isoflavone content and estrogenic activity of 25 year-old glycine max tissue cultures. | soy isoflavones are phytoestrogens which have been associated with several health benefits. in the present study, we report the production of isoflavones in a collection of 40 strains of soya cell cultures established in 1975. a large variability in the isoflavone composition was observed and high-producing strains, with an isoflavone content of up to 46.3 mg g(-1) dry wt., were found. in comparison with soybeans, many callus strains had a higher isoflavone concentration (10-40 times) and a diff ... | 2003 | 13679094 |
| methionine supplementation of processed soybeans in the rat. | | 1962 | 13865104 |
| quick serological method of classifying strains of rhizobium japonicum in nodules. | means, ura m. (u.s. department of agriculture, beltsville, md.), herbert w. johnson, and r. a. date. quick serological method of classifying strains of rhizobium japonicum in nodules. j. bacteriol. 87:547-553. 1964.-a new method of classifying strains of rhizobium japonicum serologically by utilizing the contents of nodules as antigens is described. the method differs from standard procedures only in the preparation of antigens, but it is less time-consuming. agglutination reactions of culture a ... | 1964 | 14127569 |
| studies on the digestion of soybeans. | | 1964 | 14135574 |
| prevention of coprophagy in the rat and the growth-stimulating effects of methionine, cystine and penicillin when added to diets containing unheated soybeans. | | 1965 | 14259451 |
| the effects of temperature and leaf age on the development of lesions caused by peronospora manshurica on soybeans. | | 1965 | 14272783 |
| nodulation factor for rhizobium-legume symbiosis. | valera, concepcion l. (cornell university, ithaca, n.y.), and m. alexander. nodulation factor for rhizobium-legume symbiosis. j. bacteriol. 89:1134-1139. 1965.-nodulation of excised roots of medicago sativa was enhanced by an extract of alfalfa seeds but not by several other substances. coconut water exerted a similar influence upon the formation of nodules on excised roots of glycine max and phaseolus vulgaris. direct evidence for a host factor concerned in nodule genesis was obtained by demons ... | 1965 | 14276108 |
| intestinal absorption of nitrogen and methionine from processed soybeans, in the rat. | | 1962 | 14461114 |
| effects of antibiotics, amino acids, zinc and enzymes on the growth depression in the turkey produced by raw soybeans. | | 1961 | 14465566 |
| auxin-activated nadh oxidase activity of soybean plasma membranes is distinct from the constitutive plasma membrane nadh oxidase and exhibits prion-like properties. | the hormone-stimulated and growth-related cell surface hydroquinone (nadh) oxidase activity of etiolated hypocotyls of soybeans oscillates with a period of about 24 min or 60 times per 24-h day. plasma membranes of soybean hypocotyls contain two such nadh oxidase activities that have been resolved by purification on concanavalin a columns. one in the apparent molecular weight range of 14-17 kda is stimulated by the auxin herbicide 2,4-dichlorophenoxyacetic acid (2,4-d). the other is larger and u ... | 2003 | 14503486 |
| imported longhorned weevil (coleoptera: curculionidae) injury to soybean: physiological response and injury guild-level economic injury levels. | the imported longhorned weevil, calomycterus setarius roelofs, is an occasional pest of soybean, glycine max (l.), and can cause substantial defoliation of seedling soybean when the weevil is present in large numbers. because weevil populations can reach high levels, the potential exists for significant seedling injury, so economic injury levels (eils) are needed for imported longhorned weevil on seedling soybean. because the bean leaf beetle, cerotoma trifurcata (forster), also is present on se ... | 2003 | 14503588 |
| functional characterization and expression of a cytosolic iron-superoxide dismutase from cowpea root nodules. | an iron-superoxide dismutase (fesod) with an unusual subcellular localization, vufesod, has been purified from cowpea (vigna unguiculata) nodules and leaves. the enzyme has two identical subunits of 27 kd that are not covalently bound. comparison of its n-terminal sequence (nvaginll) with the cdna-derived amino acid sequence showed that vufesod is synthesized as a precursor with seven additional amino acids. the mature protein was overexpressed in escherichia coli, and the recombinant enzyme was ... | 2003 | 14512518 |
| variability of aflatoxin and cyclopiazonic acid production by aspergillus section flavi from different substrates in argentina. | aspergillus section flavi strains isolated from peanuts, wheat and soybean grown in argentina were screened for aflatoxins (type b and g) and cyclopiazonic acid (cpa) production. aspergillus flavus was the predominant species in all substrates, although there was almost the same proportion of a. flavus and aspergillus parasiticus in peanuts. aspergillus nomius was not found. incidence of aflatoxigenic a. flavus strains was higher in peanuts (69%) than in wheat (13%) or soybeans (5%) while the ra ... | 2003 | 14527788 |
| ecology and genetics of tropical rhizobia species. | biological nitrogen fixation (bnf) technology with special reference to rhizobium-legume symbiosis is growing very rapidly with the hope of combatting world hunger by producing cheaper protein for animal and human consumption in the third world. one can see rapid progress made in the biochemistry and molecular biology of symbiotic nitrogen fixation in general; however, less progress has been made on the ecological aspects despite the fact that an enormous amount of literature is available on ino ... | 1985 | 14544047 |
| tempe fermentation: some aspects of formation of gamma-linolenic acid, proteases and vitamins. | during a tempe fermentation the concentrations of linoleic, and alpha-linolenic acids (ala) decreased while the concentration of oleic acid increased. during fatty acid synthesis rhizopus sp. produced only gamma-linolenic acid (gla) instead of ala. the amount of gla in tempe were influenced by varying external parameters. the proteolytic capacity of 36 strains of the genus rhizopus isolated from indonesian tempe or tempe inocula was examined. there was a distinct increase in the amount of free a ... | 1993 | 14545670 |
| fermented grain products, production, properties and benefits to health. | fermented foods such as japanese traditional food "miso (fermented soy bean paste)" have been shown to be rich source of micronutrients with the potential to prevent various human diseases. we have introduced effects of a new dietary supplement of fermented grain foods mixture containing extracts from wheat germ, soybeans, rice bran, tear grass, sesame, wheat, citrus lemon, green tea, green leaf extract and malted rice under the trade name of antioxidant biofactor (aob). chemical analysis of aob ... | 2003 | 14567925 |
| genetically modified soybeans: false-positive detection in fermented natural soybean (tempe). | tempe was prepared using mixtures of natural soybean and genetically modified roundup ready (rur) soybean fermented with natural rhizopus sp. the amount of rur soybean was quantified using an elisa plate test. the rur signal decreased during fermentation. in the control experiments on fermentation of non-rur soybean, the tempe gave a false-positive rur signal. the cross-reacting substance was generated only in non-rur soybean during fermentation by rhizopus sp., rhizopus oligosporus, r. oryzae, ... | 2003 | 14571970 |
| enzymatic activity and stability of d-fructose dehydrogenase and sarcosine dehydrogenase immobilized onto giant vesicles. | stable vesicles with diameters between about 1 and 10 mum were prepared by a particular emulsification technology that involved the use of the surfactants span 80 and tween 80 and the phospholipid lecithin (phosphatidylcholine from soybeans). two membrane enzymes, d-fructose dehydrogenase from gluconobacter sp. (fdh) and sarcosine dehydrogenase from pseudomonas putida (sdh), were for the first time immobilized onto the bilayer membranes of these type of vesicles; and the catalytic activity and e ... | 2003 | 14574698 |
| an ancient enzyme domain hidden in the putative beta-glucan elicitor receptor of soybean may play an active part in the perception of pathogen-associated molecular patterns during broad host resistance. | a successful defense against potential pathogens requires that a host organism is able to discriminate between self and nonself structures. soybean (glycine max l.) exploits a specific molecular pattern, a 1,6-beta-linked and 1,3-beta-branched heptaglucoside (hg), present in cell walls of the oomycetal pathogen phytophthora sojae, as a signal compound eliciting the onset of defense reactions. the specific and high affinity hg-binding site is contained in the beta-glucan-binding protein (gbp), wh ... | 2004 | 14578352 |
| sulfur assimilation in soybean ( glycine max [l.] merr.): molecular cloning and characterization of a cytosolic isoform of serine acetyltransferase. | a full-length cdna clone encoding a cytosolic isoform of serine acetyltransferase (satase; ec 2.3.1.30) was isolated by screening a soybean seedling cdna library with a (32)p-labeled expressed sequence tag. nucleotide sequence analysis of the isolated cdna revealed a single open-reading frame of 858 base pairs encoding a 30-kda polypeptide. the deduced amino acid sequence of soybean satase revealed significant homology with other plant satases. analysis of genomic dna by southern blotting indica ... | 2004 | 14586654 |
| cloning of soybean genes induced during hypersensitive cell death caused by syringolide elicitor. | syringolide elicitors produced by bacteria expressing pseudomonas syringae pv. glycinea avirulence gene d (avrd) induce hypersensitive cell death (hcd) only in soybean (glycine max [l.] merr.) plants carrying the rpg4 disease resistance gene. employing a differential display method, we isolated 13 gene fragments induced in cultured cells of a soybean cultivar harosoy (rpg4) treated with syringolides. several genes for isolated fragments were induced by syringolides in an rpg4 cultivar acme as we ... | 2004 | 14586656 |
| the genetic diversity of cultivated soybean grown in china. | cultivated soybean ( glycine max) is an economically important crop that is grown for its oil and protein products. a better knowledge of its genetic diversity will be valuable for the utilization, conservation, and management of germplasm collections. using the database of the national germplasm evaluation program of china (ngepc), we studied the geographical distribution of accessions, the genetic diversity of 15 qualitative and quantitative characters, and the genetic diversity centers of cul ... | 2004 | 14595543 |
| identification and use of actinomycetes for enhanced nodulation of soybean co-inoculated with bradyrhizobium japonicum. | the utilization of actinomycetes as potential soybean (glycine max (l.)) co-inoculants was evaluated. soil samples from carbondale and belleville, ill., were used to inoculate pre-germinated soybean plants to determine antibiotic sensitivity in the native bradyrhizobium japonicum population. sensitivity was in the order kanamycin > tetracycline > oxytetracycline > rifampicin > neomycin. antagonism by five actinomycete cultures toward seven test strains of b. japonicum was also assessed. the rank ... | 2003 | 14608383 |
| a generational study of glyphosate-tolerant soybeans on mouse fetal, postnatal, pubertal and adult testicular development. | the health safety of transgenic soybeans (glyphosate-tolerant or roundup ready) was studied using the mammalian testis (mouse model) as a sensitive biomonitor of potential toxic effects. pregnant mice were fed a transgenic soybean or a non-transgenic (conventional) diet through gestation and lactation. after weaning, the young male mice were maintained on the respective diets. at 8, 16, 26, 32, 63 and 87 days after birth, three male mice and an adult reference mouse were killed, the testes surgi ... | 2004 | 14630127 |
| [root colonization and nodulation of sinorhizobium fredii hn01dl in glycine max rhizosphere]. | rhizobox-soil microcosms studies on the colonization, dispersal and nodulation of sinorhizobium fredii hn01dl marked with luxab gene in glycine max rhizosphere showed that the colonization dynamics and the density of hn01dl in non-sterilized rhizobox-soil microcosms were different from those in sterilized rhizobox-soil microcosms. the colonization density of the former reached the maximum (8.65 log cfu.g-1 root) 12 days after the coated seeds planted, and that of the latter decreased rapidly at ... | 2003 | 14655359 |
| molecular and cell biology of a family of voltage-dependent anion channel porins in lotus japonicus. | voltage-dependent anion channels (vdacs) are generally considered as the main pathway for metabolite transport across the mitochondrial outer membrane. recent proteomic studies on isolated symbiosome membranes from legume nodules indicated that vdacs might also be involved in transport of nutrients between plants and rhizobia. in an attempt to substantiate this, we carried out a detailed molecular and cellular characterization of vdacs in lotus japonicus and soybean (glycine max). database searc ... | 2004 | 14657408 |
| effect of food processing on the fate of dna with regard to degradation and transformation capability in bacillus subtilis. | soymilk, tofu, corn masa, and cooked potato were produced from transgenic raw materials and the effect of processing on the degradation of dna was studied. major degrading factors were for soymilk and tofu the mechanical treatment of soaked soybeans and for corn masa and cooked potatoes the thermal treatment. in the processed foods no dna fragments > 1.1 kb were detected. we included in our studies the effect of the size of donor dna and length of the homologous sequence on the marker rescue tra ... | 2003 | 14666975 |
| effect of dietary fat and vitamin e on alpha-tocopherol in milk from dairy cows. | diets with different fat treatments and with 25, 125, or 250 iu of supplemental vitamin e (all-rac alpha-tocopheryl acetate)/kg of dry matter (dm) were fed for 28 d to midlactation holstein cows to determine factors affecting concentrations of alpha-tocopherol in milk. diets contained no supplemental fat or 2.25% added fat from roasted soybeans or tallow. vitamin e treatment had no effects on production, but fat supplementation increased milk yield (37.2 vs. 35.1 kg/d). cows fed rsb ate more dm ... | 2003 | 14672189 |
| retrospective study of preventive effect of maize on mortality from parkinson's disease in japan. | the findings of a negative association between past maize (zea mays) production and current parkinson's disease mortality by each prefecture in japan tends to support the hypothesis that the nutritional condition that causes niacin deficiency might protect people from parkinson's disease. specifically, the negative association between both the area planted for dried corn in 1960, 1970 or 1977 and the area planted for sweet corn in 1960 and age-adjusted death rates for parkinson's disease is ecol ... | 2003 | 14672869 |
| identification of fast and slow growing rhizobia nodulating soybean (glycine max [l.] merr) by a multiplex pcr reaction. | two dna fragments, a 730-bp and a 900-bp fragment, one homologous to host cultivar specificity genes nolbt of sinorhizobium fredii and the other one homologous to rsalpha, an insertion-like sequence present in bradyrhizobium japonicum, were generated by polymerase chain reaction (pcr) with two pairs of primers. the amount of each fragment generated by the multiplex pcr was proportional to the amount of template dna present. the amplification of the 900-bp rsalpha fragment was more sensitive, sin ... | 2003 | 14680692 |
| equol is a novel anti-androgen that inhibits prostate growth and hormone feedback. | equol (7-hydroxy-3[4'hydroxyphenyl]-chroman) is the major metabolite of the phytoestrogen daidzein, one of the main isoflavones found abundantly in soybeans and soy foods. equol may be an important biologically active molecule based on recent studies demonstrating that equol can modulate reproductive function. in this study, we examined the effects of equol on prostate growth and lh secretion and determined some of the mechanisms by which it might act. administration of equol to intact male rats ... | 2004 | 14681200 |