| the li2 mutation results in reduced subgenome expression bias in elongating fibers of allotetraploid cotton (gossypium hirsutum l.). | next generation sequencing (rna-seq) technology was used to evaluate the effects of the ligon lintless-2 (li2) short fiber mutation on transcriptomes of both subgenomes of allotetraploid cotton (gossypium hirsutum l.) as compared to its near-isogenic wild type. sequencing was performed on 4 libraries from developing fibers of li2 mutant and wild type near-isogenic lines at the peak of elongation followed by mapping and polycat categorization of rna-seq data to the reference d5 genome (g. raimond ... | 2014 | 24598808 |
| changes during leaf expansion of φpsii temperature optima in gossypium hirsutum are associated with the degree of fatty acid lipid saturation. | in this project, we hypothesize that cotton (gossypium hirsutum) leaf temperature and the responses of leaf photosynthesis to temperature will change as the leaves expand and that differences between young and mature leaves will be associated with the proportion of saturated fatty acids in thylakoid and other membrane lipids. to that end, we studied main stem leaves obtained from plants growing in a temperature controlled greenhouse and at different times in the field season. we found that young ... | 2014 | 24594393 |
| the effects of fruiting positions on cellulose synthesis and sucrose metabolism during cotton (gossypium hirsutum l.) fiber development. | cotton (gossypium hirsutum l.) boll positions on a fruiting branch vary in their contribution to yield and fiber quality. fiber properties are dependent on deposition of cellulose in the fiber cell wall, but information about the enzymatic differences in sucrose metabolism between these fruiting positions is lacking. therefore, two cotton cultivars with different sensitivities to low temperature were tested in 2010 and 2011 to quantify the effect of fruit positions (fps) on fiber quality in rela ... | 2014 | 24586807 |
| molecular characterization of cotton c-repeat/dehydration-responsive element binding factor genes that are involved in response to cold stress. | low temperature, drought and salinity are major abiotic stresses that influence survival, productivity and geographical distribution of many important crops across the globe. the c-repeat/dehydration-responsive element binding transcription factors (cbf/dreb) are important proteins involved in response to abiotic stresses in plants. in this study, twenty-one cbf genes were identified in cotton (gossypium hirsutum) by bioinformatic approach. the twenty-one cbf genes (named as ghcbf1--ghcbf21) wer ... | 2014 | 24566693 |
| construction of a complete set of alien chromosome addition lines from gossypium australe in gossypium hirsutum: morphological, cytological, and genotypic characterization. | we report the first complete set of alien addition lines of g. hirsutum . the characterized lines can be used to introduce valuable traits from g. australe into cultivated cotton. gossypium australe is a diploid wild cotton species (2n = 26, gg) native to australia that possesses valuable characteristics unavailable in the cultivated cotton gene pool, such as delayed pigment gland morphogenesis in the seed and resistances to pests and diseases. however, it is very difficult to directly transfer ... | 2014 | 24553965 |
| identification and analyses of aux-iaa target genes controlling multiple pathways in developing fiber cells of gossypium hirsutum l. | technological development led to an increased interest in systems biological approaches in plants to characterize developmental mechanism and candidate genes relevant to specific tissue or cell morphology. aux-iaa proteins are important plant-specific putative transcription factors. there are several reports on physiological response of this family in arabidopsis but in cotton fiber the transcriptional network through which aux-iaa regulated its target genes is still unknown. in-silico modelling ... | 2013 | 24497725 |
| induction and establishment of somatic embryogenesis in elite indian cotton cultivar (gossypium hirsutum l. cv khandwa-2). | embryogenesis in cotton is a difficult task due its genome dependency. we used 3 cotton cultivars (khandwa-2, g. cot. 10, and bc-68–2) and coker-312 as control for regeneration. efficient somatic embryogenesis was induced in agronomically important indian cotton cultivars, khandwa-2 and g. cot. 10. for callusing in all the cultivars, different media combinations were tried. embryogenesis was initiated on a hormone-free ms medium (msb). for embryo maturation and recovery excess of l-glutamine and ... | 2013 | 24494238 |
| modelling the structural response of cotton plants to mepiquat chloride and population density. | cotton (gossypium hirsutum) has indeterminate growth. the growth regulator mepiquat chloride (mc) is used worldwide to restrict vegetative growth and promote boll formation and yield. the effects of mc are modulated by complex interactions with growing conditions (nutrients, weather) and plant population density, and as a result the effects on plant form are not fully understood and are difficult to predict. the use of mc is thus hard to optimize. | 2014 | 24489020 |
| related to aba-insensitive3(abi3)/viviparous1 and atabi5 transcription factor coexpression in cotton enhances drought stress adaptation. | drought tolerance is an important trait being pursued by the agbiotech industry. abscisic acid (aba) is a stress hormone that mediates a multitude of processes in growth and development, water use efficiency (wue) and gene expression during seed development and in response to environmental stresses. arabidopsis b3-domain transcription factor related to aba-insensitive3 (abi3)/viviparous1 (namely atrav2) and basic leucine zipper (bzips) atabi5 or atabf3 transactivated aba-inducible promoter:gus r ... | 2014 | 24483851 |
| aberrant phenotype and transcriptome expression during fiber cell wall thickening caused by the mutation of the im gene in immature fiber (im) mutant in gossypium hirsutum l. | the immature fiber (im) mutant of gossypium hirsutum l. is a special cotton fiber mutant with non-fluffy fibers. it has low dry weight and fineness of fibers due to developmental defects in fiber secondary cell wall (scw). | 2014 | 24483163 |
| identification and functional analysis of a novel parvulin-type peptidyl-prolyl isomerase from gossypium hirsutum. | plants have developed a variety of adaptive mechanisms to cope with stresses. a novel salt-induced gene was isolated during the screening of a nacl-induced cdna library of cotton seedlings. the gene was registered as accession number ay972810 in genbank. phylogenetic analysis suggested that the protein encoded by the gene belongs to the parvulin family of peptidyl-prolyl cis/trans isomerases (ppiases, ec 5.2.1.8). northern blot analysis indicated that the mrna accumulation of ghppi was induced b ... | 2014 | 24468661 |
| genetic structure, linkage disequilibrium and association mapping of verticillium wilt resistance in elite cotton (gossypium hirsutum l.) germplasm population. | understanding the population structure and linkage disequilibrium in an association panel can effectively avoid spurious associations and improve the accuracy in association mapping. in this study, one hundred and fifty eight elite cotton (gossypium hirsutum l.) germplasm from all over the world, which were genotyped with 212 whole genome-wide marker loci and phenotyped with an disease nursery and greenhouse screening method, were assayed for population structure, linkage disequilibrium, and ass ... | 2014 | 24466016 |
| the calcium sensor ghcam7 promotes cotton fiber elongation by modulating reactive oxygen species (ros) production. | fiber elongation is the key determinant of fiber quality and output in cotton (gossypium hirsutum). although expression profiling and functional genomics provide some data, the mechanism of fiber development is still not well understood. here, a gene encoding a calcium sensor, ghcam7, was isolated based on its high expression level relative to other ghcams in fiber cells at the fast elongation stage. the level of expression of ghcam7 in the wild-type and the fuzzless/lintless mutant correspond t ... | 2014 | 24443839 |
| phytochrome rnai enhances major fibre quality and agronomic traits of the cotton gossypium hirsutum l. | simultaneous improvement of fibre quality, early-flowering, early-maturity and productivity in upland cotton (g. hirsutum) is a challenging task for conventional breeding. the influence of red/far-red light ratio on the fibre length prompted us to examine the phenotypic effects of rna interference (rnai) of the cotton phya1 gene. here we show a suppression of up to ~70% for the phya1 transcript, and compensatory overexpression of up to ~20-fold in the remaining phytochromes in somatically regene ... | 2014 | 24430163 |
| mir828 and mir858 regulate homoeologous myb2 gene functions in arabidopsis trichome and cotton fibre development. | although polyploidy is common in plants and some animals, mechanisms for functional divergence between homoeologous genes are poorly understood. myb2 gene promotes cotton fibre development and is functionally homologous to arabidopsis glabrous1 (gl1) in trichome formation. the most widely cultivated cotton is an allotetraploid (gossypium hirsutum, aadd) that contains ghmyb2a and ghmyb2d homoeologs. here we show that ghmyb2d mrna accumulates more than ghmyb2a during fibre initiation and is target ... | 2014 | 24430011 |
| association analysis of fiber quality traits and exploration of elite alleles in upland cotton cultivars/accessions (gossypium hirsutum l.). | exploring the elite alleles and germplasm accessions related to fiber quality traits will accelerate the breeding of cotton for fiber quality improvement. in this study, 99 gossypium hirsutum l. accessions with diverse origins were used to perform association analysis of fiber quality traits using 97 polymorphic microsatellite marker primer pairs. a total of 107 significant marker-trait associations were detected for three fiber quality traits under three different environments, with 70 detected ... | 2014 | 24428209 |
| functional characterization of a bidirectional plant promoter from cotton leaf curl burewala virus using an agrobacterium-mediated transient assay. | the c1 promoter expressing the ac1 gene, and v1 promoter expressing the av1 gene are located in opposite orientations in the large intergenic region of the cotton leaf curl burewala virus (clcubuv) genome. agro-infiltration was used to transiently express putative promoter constructs in nicotiana tabacum and gossypium hirsutum leaves, which was monitored by a gus reporter gene, and revealed that the bidirectional promoter of clcubuv transcriptionally regulates both the ac1 and av1 genes. the clc ... | 2014 | 24424501 |
| effects of fuzzless cottonseed phenotype on cottonseed nutrient composition in near isogenic cotton (gossypium hirsutum l.) mutant lines under well-watered and water stress conditions. | there is no information available on the effect of fuzzless seed trait on cottonseed nutrient composition (minerals, n, s, protein, and oil) under drought stress. the objective of this research was to investigate the effect of the fuzzless seed trait on cottonseed nutrients using five sets of near-isogenic lines (nils). each set consists of two lines that share the same genetic background, but differ in seed fuzziness (fuzzy, f; fuzzless, n). the near isogenic lines will enable us to compare the ... | 2013 | 24416037 |
| comparative evolutionary and developmental dynamics of the cotton (gossypium hirsutum) fiber transcriptome. | the single-celled cotton (gossypium hirsutum) fiber provides an excellent model to investigate how human selection affects phenotypic evolution. to gain insight into the evolutionary genomics of cotton domestication, we conducted comparative transcriptome profiling of developing cotton fibers using rna-seq. analysis of single-celled fiber transcriptomes from four wild and five domesticated accessions from two developmental time points revealed that at least one-third and likely one-half of the g ... | 2014 | 24391525 |
| a mads-box gene is specifically expressed in fibers of cotton (gossypium hirsutum) and influences plant growth of transgenic arabidopsis in a ga-dependent manner. | in this study, a cdna, ghmads14, encoding a typical mads-box protein with 223 amino acids was isolated from a cotton cdna library. fluorescent microscopy indicated that the ghmads14 protein was localized in the cell nucleus. ghmads14 was specifically expressed in the elongating fibers, and its expression was gradually enhanced at early stages of fiber elongation and reached its peak in 9-10 dpa fibers. overexpression of ghmads14 in arabidopsis hindered plant growth. measurement and statistical a ... | 2014 | 24374505 |
| non-destructive high-throughput dna extraction and genotyping methods for cotton seeds and seedlings. | extensive use of targeted pcr-based genotyping is precluded for many plant research laboratories by the cost and time required for dna extraction. using cotton (gossypium hirsutum) as a model for plants with medium-sized seeds, we report here manual procedures for inexpensive non-destructive high-throughput extraction of dna suitable for pcr-based genotyping of large numbers of individual seeds and seedlings. by sampling only small amounts of cotyledon tissue of ungerminated seed or young seedli ... | 2015 | 25967902 |
| construction of microsatellite-based linkage map and mapping of nectarilessness and hairiness genes in gossypium tomentosum. | gossypium tomentosum, a wild tetraploid cotton species with ad genomes, possesses genes conferring strong fibers and high heat tolerance. to effectively transfer these genes into gossypium hirsutum, an entire microsatellite (simple sequence repeat, ssr)-based genetic map was constructed using the interspecific cross of g. hirsutum x g. tomentosum (ht). we detected 1800 loci from 1347 pairs of polymorphic primers. of these, 1204 loci were grouped into 35 linkage groups at lod ≥ 4. the map covers ... | 2013 | 24371166 |
| both methylerythritol phosphate and mevalonate pathways contribute to biosynthesis of each of the major isoprenoid classes in young cotton seedlings. | in higher plants, both the methylerythritol phosphate (mep) and mevalonate (mva) pathways contribute to the biosynthesis of isoprenoids. however, despite a significant amount of research on the activity of these pathways under different conditions, the relative contribution of each to the biosynthesis of diverse isoprenoids remains unclear. in this work, we examined the formation of several classes of isoprenoids in cotton (gossypium hirsutum l.). after feeding [5,5-(2)h2]-1-deoxy-d-xylulose ([5 ... | 2014 | 24359633 |
| functional analyses of cotton (gossypium hirsutum l.) immature fiber (im) mutant infer that fiber cell wall development is associated with stress responses. | cotton fiber maturity is an important factor for determining the commercial value of cotton. how fiber cell wall development affects fiber maturity is not well understood. a comparison of fiber cross-sections showed that an immature fiber (im) mutant had lower fiber maturity than its near isogenic wild type, texas marker-1 (tm-1). the availability of the im mutant and tm-1 provides a unique way to determine molecular mechanisms regulating cotton fiber maturity. | 2013 | 24341782 |
| harmonic radar tagging for tracking movement of nezara viridula (hemiptera: pentatomidae). | harmonic radar tagging was investigated as a method for monitoring the movement of the southern green stink bug, nezara viridula (l.) (hemiptera: pentatomidae). because adhesive toxicity and tag weight limit the use of this technology, initial efforts focused on selection of the optimal adhesive and design of harmonic radar tags to reduce impact on the movement of stink bugs. a design consisting of a 6-cm-long 0.10-mm-thick silver-plated copper monopole on the anode terminal of a three-contact s ... | 2013 | 24331611 |
| rna-seq transcriptome profiling of upland cotton (gossypium hirsutum l.) root tissue under water-deficit stress. | an rna-seq experiment was performed using field grown well-watered and naturally rain fed cotton plants to identify differentially expressed transcripts under water-deficit stress. our work constitutes the first application of the newly published diploid d5 gossypium raimondii sequence in the study of tetraploid ad1 upland cotton rna-seq transcriptome analysis. a total of 1,530 transcripts were differentially expressed between well-watered and water-deficit stressed root tissues, in patterns tha ... | 2013 | 24324815 |
| ssr marker-assisted improvement of fiber qualities in gossypium hirsutum using g. barbadense introgression lines. | this study demonstrates the first practical use of csils for the transfer of fiber quality qtls into upland cotton cultivars using ssr markers without detrimentally affecting desirable agronomic characteristics. gossypium hirsutum is characterized by its high lint production and medium fiber quality compared to extra-long staple cotton g. barbadense. transferring valuable traits or genes from g. barbadense into g. hirsutum is a promising but challenging approach through a traditional interspecif ... | 2014 | 24306319 |
| gene expression profile analysis of ligon lintless-1 (li1) mutant reveals important genes and pathways in cotton leaf and fiber development. | ligon lintless-1 (li1) is a monogenic dominant mutant of gossypium hirsutum (upland cotton) with a phenotype of impaired vegetative growth and short lint fibers. despite years of research involving genetic mapping and gene expression profile analysis of li1 mutant ovule tissues, the gene remains uncloned and the underlying pathway of cotton fiber elongation is still unclear. in this study, we report the whole genome-level deep-sequencing analysis of leaf tissues of the li1 mutant. differentially ... | 2014 | 24279997 |
| the role of somatic embryogenesis receptor-like kinase 1 in controlling pollen production of the gossypium anther. | in flowering plants, male gametophytes are generated in anthers from microsporocytes. however, more evidence is needed to reveal the genetic mechanisms which regulate the differentiation and interaction of these highly specialized cells in anthers. here we report the characterization of a series of male-sterile cotton (gossypium hirsutum) mutants, including mutants with normal fertility, semi-sterility and complete sterility. these mutants are forms of transgenic cotton containing rnai vectors w ... | 2014 | 24276918 |
| genome-wide functional analysis of cotton (gossypium hirsutum) in response to drought. | cotton is one of the most important crops for its natural textile fibers in the world. however, it often suffered from drought stress during its growth and development, resulting in a drastic reduction in cotton productivity. therefore, study on molecular mechanism of cotton drought-tolerance is very important for increasing cotton production. to investigate molecular mechanism of cotton drought-resistance, we employed rna-seq technology to identify differentially expressed genes in the leaves o ... | 2013 | 24260499 |
| reflectance and cyclic electron flow as an indicator of drought stress in cotton (gossypium hirsutum). | the response and the functioning of the photosynthetic machinery of cotton, gossypium hirsutum during water stress was studied by leaf optical properties, linear (etrii) and cyclic electron flow (cef) and chlorophyll a fluorescence. we observed that in g. hirsutum, during water limitation, chlorophyll b showed the best correlation with reflectance at 731 nm and is a better indicator of drought. fv /fm was observed to be very insensitive to mild water stress. however, during severe water stress t ... | 2014 | 24246098 |
| transcriptome analysis reveals crosstalk of responsive genes to multiple abiotic stresses in cotton (gossypium hirsutum l.). | abiotic stress is a major environmental factor that limits cotton growth and yield, moreover, this problem has become more and more serious recently, as multiple stresses often occur simultaneously due to the global climate change and environmental pollution. in this study, we sought to identify genes involved in diverse stresses including abscisic acid (aba), cold, drought, salinity and alkalinity by comparative microarray analysis. our result showed that 5790, 3067, 5608, 778 and 6148 transcri ... | 2013 | 24224045 |
| the dual functions of wlim1a in cell elongation and secondary wall formation in developing cotton fibers. | lin-11, isl1 and mec-3 (lim)-domain proteins play pivotal roles in a variety of cellular processes in animals, but plant lim functions remain largely unexplored. here, we demonstrate dual roles of the wlim1a gene in fiber development in upland cotton (gossypium hirsutum). wlim1a is preferentially expressed during the elongation and secondary wall synthesis stages in developing fibers. overexpression of wlim1a in cotton led to significant changes in fiber length and secondary wall structure. comp ... | 2013 | 24220634 |
| the promoter structure differentiation of a myb transcription factor rlc1 causes red leaf coloration in empire red leaf cotton under light. | the red leaf coloration of empire red leaf cotton (erlc) (gossypium hirsutum l.), resulted from anthocyanin accumulation in light, is a well known dominant agricultural trait. however, the underpin molecular mechanism remains elusive. to explore this, we compared the molecular biological basis of anthocyanin accumulation in both erlc and the green leaf cotton variety ccri 24 (gossypium hirsutum l.). introduction of r2r3-myb transcription factor rosea1, the master regulator anthocyanin biosynthes ... | 2013 | 24205014 |
| changes in the cell wall and cellulose content of developing cotton fibers investigated by ftir spectroscopy. | fourier transform infrared (ftir) spectra of cotton fibers harvested at different stages of development were acquired using universal attenuated total reflectance ftir (uatr-ftir). the main goal of the study was to monitor cell wall changes occurring during different phases of cotton fiber development. two cultivars of gossypium hirsutum l. were planted in a greenhouse (texas marker-1 and tx55). on the day of flowering, individual flowers were tagged and bolls were harvested. from fibers harvest ... | 2014 | 24188832 |
| identification and characterization of (e)-β-caryophyllene synthase and α/β-pinene synthase potentially involved in constitutive and herbivore-induced terpene formation in cotton. | cotton (gossypium hirsutum l.) plants damaged by insects emit a blend of volatiles, including monoterpenes and sesquiterpenes, which can directly repel herbivores and/or indirectly protect the plant by attracting natural enemies of the herbivores. to understand the molecular basis of terpene biosynthesis and regulation in cotton, two terpene synthase genes, ghtps1 and ghtps2, were heterologously expressed and characterized. recombinant ghtps1 accepted farnesyl pyrophosphate as substrate and prod ... | 2013 | 24184450 |
| genetic diversity and population structure in the us upland cotton (gossypium hirsutum l.). | genetic diversity and population structure in the us upland cotton was established and core sets of allelic richness were identified for developing association mapping populations in cotton. elite plant breeding programs could likely benefit from the unexploited standing genetic variation of obsolete cultivars without the yield drag typically associated with wild accessions. a set of 381 accessions comprising 378 upland (gossypium hirsutum l.) and 3 g. barbadense l. accessions of the united stat ... | 2014 | 24170350 |
| mapping and genomic targeting of the major leaf shape gene (l) in upland cotton (gossypium hirsutum l.). | a major leaf shape locus (l) was mapped with molecular markers and genomically targeted to a small region in the d-genome of cotton. by using expression analysis and candidate gene mapping, two lmi1 -like genes are identified as possible candidates for leaf shape trait in cotton. leaf shape in cotton is an important trait that influences yield, flowering rates, disease resistance, lint trash, and the efficacy of foliar chemical application. the leaves of okra leaf cotton display a significantly ... | 2014 | 24158249 |
| generation and analysis of a large-scale expressed sequence tag database from a full-length enriched cdna library of developing leaves of gossypium hirsutum l. | cotton (gossypium hirsutum l.) is one of the world's most economically-important crops. however, its entire genome has not been sequenced, and limited resources are available in genbank for understanding the molecular mechanisms underlying leaf development and senescence. | 2013 | 24146870 |
| cellulose synthases: new insights from crystallography and modeling. | detailed information about the structure and biochemical mechanisms of cellulose synthase (cels) proteins remained elusive until a complex containing the catalytic subunit (bcsa) of cels from rhodobacter sphaeroides was crystalized. additionally, a 3d structure of most of the cytosolic domain of a plant cels (ghcesa1 from cotton, gossypium hirsutum) was produced by computational modeling. this predicted structure contributes to our understanding of how plant cels proteins may be similar and diff ... | 2014 | 24139443 |
| linkage mapping and expression analysis of mirnas and their target genes during fiber development in cotton. | micrornas (mirnas) are small, endogenously expressed, non-coding rna molecules involved in gene transcription and expression that combine with specific mrna site of target genes to inhibit protein synthesis or degrade mrna. since the first plant mirna was reported in 2002, numerous new mirnas and their targets have been discovered via high-throughput sequencing and computational approaches. however, the genetic variation of mirna genes is poorly understood due to the lack of mirna-specific dna m ... | 2013 | 24131852 |
| transcriptome dynamics during fibre development in contrasting genotypes of gossypium hirsutum l. | understanding the contribution of genetic background in fibre quality traits is important for the development of future cotton varieties with superior fibre quality. we used affymetrix microarray (santa clara, ca) and roche 454 gsflx (branford, ct) for comparative transcriptome analysis between two superior and three inferior genotypes at six fibre developmental stages. microarray-based analysis of variance (anova) for 89 microarrays encompassing five contrasting genotypes and six developmental ... | 2014 | 24119257 |
| bac-pool sequencing and analysis of large segments of a12 and d12 homoeologous chromosomes in upland cotton. | although new and emerging next-generation sequencing (ngs) technologies have reduced sequencing costs significantly, much work remains to implement them for de novo sequencing of complex and highly repetitive genomes such as the tetraploid genome of upland cotton (gossypium hirsutum l.). herein we report the results from implementing a novel, hybrid sanger/454-based bac-pool sequencing strategy using minimum tiling path (mtp) bacs from ctg-3301 and ctg-465, two large genomic segments in a12 and ... | 2013 | 24116150 |
| comparative transcriptome analysis between somatic embryos (ses) and zygotic embryos in cotton: evidence for stress response functions in se development. | as a product of asexual reproduction in plants, the somatic embryo (se) differentiates into a new plantlet via a zygotic embryogenesis-like process. here, we present the phenotypic and cellular differences between ses and zygotic embryos (zes) revealed by histological section scanning using three parallel development stages of the two types of embryos of cotton (gossypium hirsutum cv. yz1), including globular, torpedo and cotyledonary-stages. to identify the molecular characteristics of se devel ... | 2014 | 24112122 |
| isolation and characterization of terpene synthases in cotton (gossypium hirsutum). | cotton plants accumulate gossypol and related sesquiterpene aldehydes, which function as phytoalexins against pathogens and feeding deterrents to herbivorous insects. however, to date little is known about the biosynthesis of volatile terpenes in this crop. herein is reported that 5 monoterpenes and 11 sesquiterpenes from extracts of a glanded cotton cultivar, gossypium hirsutum cv. ccri12, were detected by gas chromatography-mass spectrometry (gc-ms). by est data mining combined with rapid ampl ... | 2013 | 24074555 |
| genome-wide analysis of small rnas reveals eight fiber elongation-related and 257 novel micrornas in elongating cotton fiber cells. | micrornas (mirnas) and other types of small regulatory rnas play critical roles in the regulation of gene expression at the post-transcriptional level in plants. cotton is one of the most economically important crops, but little is known about the roles of mirnas during cotton fiber elongation. | 2013 | 24044642 |
| accumulation of biomass and mineral elements with calendar time by cotton: application of the expanded growth model. | accumulation of plant biomass (mg ha(-1)) with calendar time (wk) occurs as a result of photosynthesis for green land-based plants. a corresponding accumulation of mineral elements (kg ha(-1)) such as nitrogen, phosphorus, and potassium occurs from the soil through plant roots. field data from literature for the warm-season annual cotton (gossypium hirsutum l.) are used in this analysis. the expanded growth model is used to describe accumulation of biomass and mineral elements with calendar time ... | 2013 | 24039802 |
| isolation and expression profiling of ghnac transcription factor genes in cotton (gossypium hirsutum l.) during leaf senescence and in response to stresses. | nac (nam, ataf, and cuc) is a plant-specific transcription factor family with diverse roles in plant development and stress regulation. in this report, stress-responsive nac genes (ghnac8-ghnac17) isolated from cotton (gossypium hirsutum l.) were characterised in the context of leaf senescence and stress tolerance. the characterisation of nac genes during leaf senescence has not yet been reported for cotton. based on the sequence characterisation, these ghnacs could be classified into three grou ... | 2013 | 24036432 |
| variations in seed protein content of cotton (gossypium hirsutum l.) mutant lines by in vivo and in vitro mutagenesis. | the present work describes the influence of gamma irradiation (gr), ethyl methane sulphonate (ems) and sodium azide (sa) treatment on yield and protein content of selected mutant lines of cotton. seeds of mcu 5 and mcu 11 were exposed to gamma rays (gr), ethyl methane sulphonate (ems) and sodium azide (sa). lower dose of gamma irradiation (100-500 gy), 10-50 mm ems and sa at lower concentration effectively influences in improving the yield and protein content. significant increase in yield (258. ... | 2013 | 24006801 |
| isolation, characterization and mapping of genes differentially expressed during fibre development between gossypium hirsutum and g. barbadense by cdna-srap. | gossypium hirsutum and g. barbadense are two cultivated tetraploid cotton species with differences in fibre quality. the fibre of g. barbadense is longer, stronger and finer than that of g. hirsutum. to isolate genes expressed differently between the two species during fibre development, cdna-srap (sequence-related amplified polymorphism) was applied. this technique was used to analyse genes at different stages of fibre development in g. hirsutum cv. emian22 and g. barbadense acc. 3-79, the pare ... | 2013 | 23970073 |
| response of mirnas and their targets to salt and drought stresses in cotton (gossypium hirsutum l.). | micrornas (mirnas) are an important gene regulator, controlling almost all biological and metabolic processes, in both plants and animals. in this study, we investigated the effect of drought and salinity stress on the expression of mirnas and their targets in cotton (gossypium hirsutum l.). our results show that the expression change of mirnas and their targets were dose-dependent and tissue-dependent under salinity and drought conditions. the expression of mirnas in leaf was down-regulated und ... | 2013 | 23948080 |
| the complete mitochondrial genome of gossypium hirsutum and evolutionary analysis of higher plant mitochondrial genomes. | mitochondria are the main manufacturers of cellular atp in eukaryotes. the plant mitochondrial genome contains large number of foreign dna and repeated sequences undergone frequently intramolecular recombination. upland cotton (gossypium hirsutum l.) is one of the main natural fiber crops and also an important oil-producing plant in the world. sequencing of the cotton mitochondrial (mt) genome could be helpful for the evolution research of plant mt genomes. | 2013 | 23940520 |
| evaluation and selection of reliable reference genes for gene expression under abiotic stress in cotton (gossypium hirsutum l.). | reference genes are critical for normalization of the gene expression level of target genes. the widely used housekeeping genes may change their expression levels at different tissue under different treatment or stress conditions. therefore, systematical evaluation on the housekeeping genes is required for gene expression analysis. up to date, no work was performed to evaluate the housekeeping genes in cotton under stress treatment. in this study, we chose 10 housekeeping genes to systematically ... | 2013 | 23933278 |
| genome-wide analysis of small rna and novel microrna discovery during fiber and seed initial development in gossypium hirsutum. l. | cotton is the source of the most important, renewable natural textile fiber and oil in the world. micrornas (mirnas) are endogenous, non-coding, approximately 18-24 nucleotides long rnas and function in the negative regulation of their target genes. two mostly overlapping libraries of small rna molecules were constructed and sequenced, and served as repetition sets of data to identify mirnas involved in fiber initiation and seed development. the d genome sequence of gossypium raimondii was used ... | 2013 | 23922789 |
| molecular characterisation of two homoeologous elicitor-responsive lipin genes in cotton. | the identification and molecular characterisation of two lipin-like gene copies (ghlipn) in cotton, gossypium hirsutum, an allotetraploid derived from two progenitor diploid gossypium species, is described. sequence analyses of the ghlipn copies, designated ghlipn-1 and -2, revealed that they contain 11 exons, separated by ten introns. they each have a 2,643 bp open reading frame that encodes 880 aa proteins, and share a 97.7 and 95.5 % sequence similarity at the translated nucleotide and amino ... | 2013 | 23897433 |
| cloning and characterization of a flo/lfy ortholog in gossypium hirsutum l. | ghlfy was cloned from g. hirsutum l. its expression, subcellular localization, and function were analyzed, as well as the in vivo regulation of ghlfy by the mads-box protein soc1 (ghsoc1). | 2013 | 23893068 |
| characterization and expression analysis of a fiber differentially expressed fasciclin-like arabinogalactan protein gene in sea island cotton fibers. | fasciclin-like arabinogalactan (fla) protein is a cell-wall-associated protein playing crucial roles in regulating plant growth and development, and it was characterized in different plants including upland cotton (gossypium hirsutum l.). in cdna-aflp analysis of 25 dpa (days post anthesis) fiber mrna, two fla gene-related transcripts exhibit differential expression between sea island cotton (g. barbadense l.) and upland cotton. based on the transcript-derived fragment, race-pcr and realtime pcr ... | 2013 | 23875019 |
| single and multiple in-season measurements as indicators of at-harvest cotton boll damage caused by verde plant bug (hemiptera: miridae). | the ability to monitor verde plant bug, creontiades signatus distant (hemiptera: miridae), and the progression of cotton, gossypium hirsutum l., boll responses to feeding and associated cotton boll rot provided opportunity to assess if single in-season measurements had value in evaluating at-harvest damage to bolls and if multiple in-season measurements enhanced their combined use. one in-season verde plant bug density measurement, three in-season plant injury measurements, and two at-harvest da ... | 2013 | 23865196 |
| compensation of lygus hesperus induced preflower fruit loss in cotton. | a 3-yr field study quantified the compensatory ability of cotton (gossypium hirsutum l.) to preflower fruit damage by lygus hesperus knight in the texas high plains under limited irrigation. experiments were designed to achieve varying levels of preflower fruit loss by augmenting lygus bug populations using nymphal bugs reared in a laboratory colony. treatments included 1) three bugs per plant (3pp), 2) one bug per plant (1pp), 3) naturally occurring background bug density or untreated control ( ... | 2013 | 23865185 |
| qtl analysis for early-maturing traits in cotton using two upland cotton (gossypium hirsutum l.) crosses. | making use of the markers linked closely to qtl for early-maturing traits for mas (marker-assisted selection) is an effective method for the simultaneous improvement of early maturity and other properties in cotton. in this study, two f2 populations and their f2:3 families were generated from the two upland cotton (gossypium hirsutum l.) crosses, baimian2 × tm-1 and baimian2 × cir12. qtl for early-maturing traits were analyzed using f2:3 families. a total of 54 qtl (31 suggestive and 23 signific ... | 2013 | 23853509 |
| tolerance to feeding damage by cotton fleahopper (hemiptera: miridae) among genotypes representing adapted germplasm pools of united states upland cotton. | cotton fleahopper [pseudatomoscelis seriatus (reuter)] (hemiptera: miridae) is one of the most damaging insect pests of cotton (gossypium hirsutum l.) in texas and oklahoma because of their feeding on small floral buds which are termed squares. damage to early season squares can reduce yield, delay crop maturity and increase the risk of crop loss because of late season insect pests and adverse weather. insecticide applications are the only control tactic. the objectives of this study were to det ... | 2013 | 23786099 |
| impact of twospotted spider mite (acari: tetranychidae) duration of infestation on cotton seedlings. | the yield response of cotton, gossypium hirsutum l., to twospotted spider mite, tetranychus urticae koch, duration of infestation during the seedling stage was measured at starkville and stoneville, ms, during 2010 and 2011. the treatments included a noninfested control, infestations lasting for 7, 14, 21, or 28 d, and a season long infested control. twospotted spider mites from a greenhouse colony were inoculated on all of the infested cotton plots during the three-leaf stage. applications of m ... | 2013 | 23786075 |
| large-scale resource development in gossypium hirsutum l. by 454 sequencing of genic-enriched libraries from six diverse genotypes. | the sequence information has been proved to be an essential genomic resource in case of crop plants for their genetic improvement and better utilization by humans. to dissect the gossypium hirsutum genome for large-scale development of genomic resources, we adopted hypomethylated restriction-based genomic enrichment strategy to sequence six diverse genotypes. approximately 5.2-gb data (more than 18.36 million reads) was generated which, after assembly, represents nearly 1.27-gb genomic sequences ... | 2013 | 23782852 |
| transcript profiling by microarray and marker analysis of the short cotton (gossypium hirsutum l.) fiber mutant ligon lintless-1 (li1). | cotton fiber length is very important to the quality of textiles. understanding the genetics and physiology of cotton fiber elongation can provide valuable tools to the cotton industry by targeting genes or other molecules responsible for fiber elongation. ligon lintless-1 (li1) is a monogenic mutant in upland cotton (gossypium hirsutum) which exhibits an early cessation of fiber elongation resulting in very short fibers (< 6 mm) at maturity. this presents an excellent model system for studying ... | 2013 | 23767687 |
| molecular cloning and function analysis of two squamosa-like mads-box genes from gossypium hirsutum l. | the mads-box genes encode a large family of transcription factors having diverse roles in plant development. the squamosa (squa)/apetala1 (ap1)/fruitfull (ful) subfamily genes are essential regulators of floral transition and floral organ identity. here we cloned two mads-box genes, ghmads22 and ghmads23, belonging to the squa/ap1/ful subgroup from gossypium hirsutum l. phylogenetic analysis and sequence alignment showed that ghmads22 and ghmads23 belonged to the euful and euap1 subclades, respe ... | 2013 | 23718551 |
| transcriptome profiling reveals auxin and cytokinin regulating somatic embryogenesis in different sister lines of cotton cultivar ccri24. | to get a broader view on the molecular mechanisms underlying somatic embryogenesis (se) in cotton (gossypium hirsutum l.), global analysis of cotton transcriptome dynamics during se in different sister lines was performed using rna-seq. a total of 204 349 unigenes were detected by de novo assembly of the 214 977 462 illumina reads. the quantitative reverse transcription-polymerase chain reaction (qrt-pcr) measurements were positively correlated with the rna-seq results for almost all the tested ... | 2013 | 23710882 |
| resolving multiple host use of an emergent pest of cotton with microsatellite data and chloroplast markers (creontiades dilutus stål; hemiptera, miridae). | following the global uptake of transgenic cotton several hemipteran pests have emerged as primary targets for pesticide control. previous research on one such emergent pest: the green mirid, creontiades dilutus, indicated differential use of two crop hosts, cotton (gossypium hirsutum, malvaceae) and lucerne (alfalfa) (medicago sativa, fabaceae). we tested the hypothesis that this apparent demographic independence of lucerne and cotton inhabiting mirids is the result of cryptic species being asso ... | 2013 | 23702301 |
| selection and characterization of a novel photoperiod-sensitive male sterile line in upland cotton. | upland cotton (gossypium hirsutum l.) shows strong heterosis. however, heterosis is not widely utilized owing to the high cost of hybrid seed production. creation of a photoperiod-sensitive genetic male sterile line could substantially reduce the cost of hybrid seed production in upland cotton. such a mutant with virescent marker was found by space mutation in near-earth orbit and its traits had been stable after 4 years of selection in anyang and sanya, china. this mutant was fertile with an 11 ... | 2013 | 23691935 |
| how predictable are the behavioral responses of insects to herbivore induced changes in plants? responses of two congeneric thrips to induced cotton plants. | changes in plants following insect attack are referred to as induced responses. these responses are widely viewed as a form of defence against further insect attack. in the current study we explore whether it is possible to make generalizations about induced plant responses given the unpredictability and variability observed in insect-plant interactions. experiments were conducted to test for consistency in the responses of two congeneric thrips, frankliniella schultzei trybom and frankliniella ... | 2013 | 23691075 |
| using genome-referenced expressed sequence tag assembly to analyze the origin and expression patterns of gossypium hirsutum transcripts. | we assembled a total of 297,239 gossypium hirsutum (gh, a tetraploid cotton, aadd) expressed sequence tag (est) sequences that were available in the national center for biotechnology information database, with reference to the recently published g. raimondii (gr, a diploid cotton, dd) genome, and obtained 49,125 unigenes. the average lengths of the unigenes were increased from 804 and 791 bp in two previous est assemblies to 1,019 bp in the current analysis. the number of putative cotton unigene ... | 2013 | 23675784 |
| cotton ghbak1 mediates verticillium wilt resistance and cell death. | virus-induced gene silencing (vigs) offers a powerful approach for functional analysis of individual genes by knocking down their expression. we have adopted this approach to dissect gene functions in cotton resistant to verticillium wilt, one of the most devastating diseases worldwide. we showed here that highly efficient vigs was obtained in a cotton breeding line (ca4002) with partial resistance to verticillium wilt, and ghmkk2 and ghve1 are required for its resistance to verticillium wilt. a ... | 2013 | 23675706 |
| effects of rootstocks on cryotolerance and overwintering survivorship of genic male sterile lines in upland cotton (gossypium hirsutum l.). | grafting desirable scion on stress-tolerant rootstocks provides an opportunity to improve the cryotolerance of scion. genic male sterile (gms) lines of plant could be used as sterile line and maintainer in breeding, and they have the conspicuous characteristics that the fertility of which is easy to regain but hard to maintain by sexual reproduction. in order to maintain the fertility of gms cotton by means of its perennial growth on the basis of frostless winters in nanning, guangxi autonomous ... | 2013 | 23667634 |
| cotton ghcki disrupts normal male reproduction by delaying tapetum programmed cell death via inactivating starch synthase. | anther infertility under high temperature (ht) conditions is a critical factor contributing to yield loss in cotton (gossypium hirsutum). using large-scale expression profile sequencing, we studied the effect of ht on cotton anther development. our analysis revealed that altered carbohydrate metabolism or disrupted tapetal programmed cell death (pcd) underlie anther sterility. expression of the gossypium hirsutum casein kinase i (ghcki) gene, which encodes a homolog of casein kinase i (cki), was ... | 2013 | 23662698 |
| neonicotinoid insecticides alter induced defenses and increase susceptibility to spider mites in distantly related crop plants. | chemical suppression of arthropod herbivores is the most common approach to plant protection. insecticides, however, can cause unintended, adverse consequences for non-target organisms. previous studies focused on the effects of pesticides on target and non-target pests, predatory arthropods, and concomitant ecological disruptions. little research, however, has focused on the direct effects of insecticides on plants. here we demonstrate that applications of neonicotinoid insecticides, one of the ... | 2013 | 23658754 |
| cotton anngh3 encoding an annexin protein is preferentially expressed in fibers and promotes initiation and elongation of leaf trichomes in transgenic arabidopsis. | the annexins are a multifamily of calcium-regulated phospholipid-binding proteins. to investigate the roles of annexins in fiber development, four genes encoding putative annexin proteins were isolated from cotton (gossypium hirsutum) and designated anngh3, anngh4, anngh5, and anngh6. quantitative reverse transcription-polymerase chain reaction (qrt-pcr) results indicated that anngh3, anngh4, and anngh5 were preferentially expressed in fibers, while the transcripts of anngh6 were predominantly a ... | 2013 | 23651035 |
| cotton gasl genes encoding putative gibberellin-regulated proteins are involved in response to ga signaling in fiber development. | gast (ga-stimulated transcript)-like genes have been reported as targets of ga regulation in some plant species. in this study, we isolated seven gast-like cdnas from cotton (gossypium hirsutum) cdna libraries (designated as ghgasl1-ghgasl7). meanwhile, the genomic dna clones corresponding to the seven ghgasl genes were isolated by using pcr amplification technique. analysis of gene structure revealed that four genes (ghgasl1/3/5/6) contain two exons and one intron, while the rest have four exon ... | 2013 | 23645033 |
| chloroplast-targeted expression of recombinant crystal-protein gene in cotton: an unconventional combat with resistant pests. | plants transformed with single bt gene are liable to develop insect resistance and this has already been reported in a number of studies carried out around the world where bt cotton was cultivated on commercial scale. later, it was envisaged to transform plants with more than one bt genes in order to combat with resistant larvae. this approach seems valid as various bt genes possess different binding domains which could delay the likely hazards of insect resistance against a particular bt toxin. ... | 2013 | 23643479 |
| cotton bracts are adapted to a microenvironment of concentrated co2 produced by rapid fruit respiration. | elucidation of the mechanisms by which plants adapt to elevated co2 is needed; however, most studies of the mechanisms investigated the response of plants adapted to current atmospheric co2. the rapid respiration rate of cotton (gossypium hirsutum) fruits (bolls) produces a concentrated co2 microenvironment around the bolls and bracts. it has been observed that the intercellular co2 concentration of a whole fruit (bract and boll) ranges from 500 to 1300 µmol mol(-1) depending on the irradiance, ... | 2013 | 23625144 |
| comparative expression profiling of mirna during anther development in genetic male sterile and wild type cotton. | genetic male sterility (gms) in cotton (gossypium hirsutum) plays an important role in the utilization of hybrid vigor. however, the molecular mechanism of the gms is still unclear. while numerous studies have demonstrated that micrornas (mirna) regulate flower and anther development, whether different small rna regulations exist in gms and its wild type is unclear. a deep sequencing approach was used to investigate the global expression and complexity of small rnas during cotton anther developm ... | 2013 | 23597285 |
| two modified rna extraction methods compatible with transcript profiling and gene expression analysis for cotton roots. | efficient isolation of high-quality rna is of prime importance for optimal transcript profiling results and further gene expression analysis. however, it is difficult for cotton roots because of lower-than-average rna content and high content of polysaccharides, polyphenols, and other secondary metabolites. to develop simple and reliable protocols for high-quality rna extraction from cotton roots for transcript profiling and gene expression analysis, some modifications were introduced to a repor ... | 2013 | 23581784 |
| mapping heterotic loci for yield and agronomic traits using chromosome segment introgression lines in cotton. | in the present study, a set of chromosome segment introgression lines (csils) using gossypium hirsutum l. tm-1 as the recipient parent and g. barbadense hai7124 as the donor parent were used to explore the genetic basis of heterosis for interspecific hybrids. two sets of f₁ populations individually derived from csils crossing with both parents were configured to investigate heterotic loci (hl) and substitution effect loci (sl). a total of 58 hl and 39 sl were identified in 3 years. one stable hl ... | 2013 | 23570369 |
| analysis of flavonoids and the flavonoid structural genes in brown fiber of upland cotton. | as a result of changing consumer preferences, cotton (gossypium hirsutum l.) from varieties with naturally colored fibers is becoming increasingly sought after in the textile industry. the molecular mechanisms leading to colored fiber development are still largely unknown, although it is expected that the color is derived from flavanoids. | 2013 | 23527031 |
| genotypic variability for root/shoot parameters under water stress in some advanced lines of cotton (gossypium hirsutum l.). | research pertaining to genetic variability parameters, heritability, and genotypic, phenotypic, simple, and environmental correlations for various seedling traits in five elite advanced cotton (gossypium hirsutum l.) lines (fh-113, fh-114, fh-941, fh-942, and fh-2015) and one check (cim-496) was carried out during october and november 2010 under greenhouse conditions at the cotton research institute (faisalabad, pakistan). material was raised in plastic tubes with a randomized complete block des ... | 2013 | 23512672 |
| integrated metabolomics and genomics analysis provides new insights into the fiber elongation process in ligon lintless-2 mutant cotton (gossypium hirsutum l.). | the length of cotton fiber is an important agronomic trait characteristic that directly affects the quality of yarn and fabric. the cotton (gossypium hirsutum l.) fiber mutation, ligon lintless-2, is controlled by a single dominant gene (li(2)) and results in extremely shortened lint fibers on mature seeds with no visible pleiotropic effects on vegetative growth and development. the li(2) mutant phenotype provides an ideal model system to study fiber elongation. to understand metabolic processes ... | 2013 | 23497242 |
| variations and transmission of qtl alleles for yield and fiber qualities in upland cotton cultivars developed in china. | cotton is the world's leading cash crop, and genetic improvement of fiber yield and quality is the primary objective of cotton breeding program. in this study, we used various approaches to identify qtls related to fiber yield and quality. firstly, we constructed a four-way cross (4wc) mapping population with four base core cultivars, stoneville 2b, foster 6, deltapine 15 and zhongmiansuo no.7 (cri 7), as parents in chinese cotton breeding history and identified 83 qtls for 11 agronomic and fibe ... | 2013 | 23468939 |
| polycat: a resource for genome categorization of sequencing reads from allopolyploid organisms. | read mapping is a fundamental part of next-generation genomic research but is complicated by genome duplication in many plants. categorizing dna sequence reads into their respective genomes enables current methods to analyze polyploid genomes as if they were diploid. we present polycat-a pipeline for mapping and categorizing all types of next-generation sequence data produced from allopolyploid organisms. polycat uses gsnap's single-nucleotide polymorphism (snp)-tolerant mapping to minimize the ... | 2013 | 23450226 |
| verde plant bug (hemiptera: miridae) feeding injury to cotton bolls characterized by boll age, size, and damage ratings. | the verde plant bug, creontiades signatus (distant), has been present in south texas for several years but has more recently been documented as an economic threat to cultivated cotton, (gossypium hirsutum l. our studies over 2 yr (2009 and 2010) and two locations (weslaco and corpus christi, tx) investigated feeding-injury of the verde plant bug to a range of cotton boll age classes defined by boll diameter and accumulated degree-days (anthesis to the time of infesting) for first-position cotton ... | 2013 | 23448031 |
| reproductive dormancy in boll-weevil from populations of the midwest of brazil. | the boll weevil (anthonomus grandis boheman) (coleoptera: curculionidae) is an introduced pest in brazil, which in 30 yr has successfully expanded to various eco-regions and became the most important pest of cotton (gossypium hirsutum, malvaceae). given the limited knowledge about the adaptive mechanisms that allowed successful establishment of the pest population in a tropical region, in this work we studied the potential of the midwest population of boll weevils to enter a reproductive dormanc ... | 2013 | 23448019 |
| identification of mitochondrial dna sequence variation and development of single nucleotide polymorphic markers for cms-d8 in cotton. | cytoplasmic male sterility (cms), which is a maternally inherited trait and controlled by novel chimeric genes in the mitochondrial genome, plays a pivotal role in the production of hybrid seed. in cotton, no pcr-based marker has been developed to discriminate cms-d8 (from gossypium trilobum) from its normal upland cotton (ad1, gossypium hirsutum) cytoplasm. the objective of the current study was to develop pcr-based single nucleotide polymorphic (snp) markers from mitochondrial genes for the cm ... | 2013 | 23435734 |
| genetic diversity analysis in opal cotton hybrids based on ssr, issr, and rapd markers. | cotton is one of the most economically important crops in iran; hybridization is a means to increase the genetic diversity and obtain new elite cultivars in this crop. we examined agronomic characteristics and molecular genetic diversity in the opal cotton (gossypium hirsutum) cultivar and in f(2) progenies. ten homo-primers and seven hetero-primers of 26 rapd primers produced 261 reproducible bands, with an average of 4.18 bands per primer and 22% polymorphism. the opb12/oph08 primer gave the h ... | 2013 | 23408413 |
| transcriptomic analysis of differentially expressed genes during anther development in genetic male sterile and wild type cotton by digital gene-expression profiling. | cotton (gossypium hirsutum) anther development involves a diverse range of gene interactions between sporophytic and gametophytic tissues. however, only a small number of genes are known to be specifically involved in this developmental process and the molecular mechanism of the genetic male sterility (gms) is still poorly understand. to fully explore the global gene expression during cotton anther development and identify genes related to male sterility, a digital gene expression (dge) analysis ... | 2013 | 23402279 |
| carbon dioxide diffusion across stomata and mesophyll and photo-biochemical processes as affected by growth co2 and phosphorus nutrition in cotton. | nutrients such as phosphorus may exert a major control over plant response to rising atmospheric carbon dioxide concentration (co2), which is projected to double by the end of the 21st century. elevated co2 may overcome the diffusional limitations to photosynthesis posed by stomata and mesophyll and alter the photo-biochemical limitations resulting from phosphorus deficiency. to evaluate these ideas, cotton (gossypium hirsutum) was grown in controlled environment growth chambers with three level ... | 2013 | 23384758 |
| small rna and degradome sequencing reveal complex mirna regulation during cotton somatic embryogenesis. | micrornas (mirnas) are endogenous non-coding ~21 nucleotide rnas that regulate gene expression at the transcriptional and post-transcriptional levels in plants and animals. they play an important role in development, abiotic stress, and pathogen responses. mirnas with their targets have been widely studied in model plants, but limited knowledge is available on the small rna population of cotton (gossypium hirsutum)-an important economic crop, and global identification of related targets through ... | 2013 | 23382553 |
| genetic mapping and characteristics of genes specifically or preferentially expressed during fiber development in cotton. | cotton fiber is an ideal model to study cell elongation and cell wall construction in plants. during fiber development, some genes and proteins have been reported to be specifically or preferentially expressed. mapping of them will reveal the genomic distribution of these genes, and will facilitate selection in cotton breeding. based on previous reports, we designed 331 gene primers and 164 protein primers, and used single-strand conformation polymorphism (sscp) to map and integrate them into ou ... | 2013 | 23372723 |
| genes expressed in cotton (gossypium hirsutum) buds isolated with a subtractive library. | a subtractive cdna library from cotton buds was constructed to prospect for differentially expressed genes related to early bud development. a library was constructed and 768 cdna sequences were obtained, comprising 168 clusters, with 126 contigs and 42 singlets. both the gossypium as well as arabidopsis databases were utilized for the in silico analysis, since some genes identified in cotton have not yet been studied for functionality, although they have homology with genes from other species. ... | 2013 | 23359022 |
| development of a plant-based threshold for tarnished plant bug (hemiptera: miridae) in cotton. | the tarnished plant bug, lygus lineolaris (palisot de beauvois), is an important pest of cotton, gossypium hirsutum l., in the mid-southern united states. it is exclusively controlled with foliar insecticide applications, and sampling methods and thresholds need to be revisited. the current experiment was designed to establish a plant-based threshold during the flowering period of cotton development. experiments were conducted in mississippi in 2005 and 2006, arkansas in 2005, and louisiana in 2 ... | 2012 | 23356065 |
| a fasciclin-like arabinogalactan protein, ghfla1, is involved in fiber initiation and elongation of cotton. | arabinogalactan proteins (agps) are involved in many aspects of plant development. in this study, biochemical and genetic approaches demonstrated that agps are abundant in developing fibers and may be involved in fiber initiation and elongation. to further investigate the role of agps during fiber development, a fasciclin-like arabinogalactan protein gene (ghfla1) was identified in cotton (gossypium hirsutum). overexpression of ghfla1 in cotton promoted fiber elongation, leading to an increase i ... | 2013 | 23349362 |
| synergistic effects of ghsod1 and ghcat1 overexpression in cotton chloroplasts on enhancing tolerance to methyl viologen and salt stresses. | in plants, cuzn superoxide dismutase (cuznsod, ec l.15.1.1), ascorbate peroxidase (apx, ec 1.11.1.11), and catalase (cat, ec l.11.1.6) are important scavengers of reactive oxygen species (ros) to protect the cell from damage. in the present study, we isolated three homologous genes (ghsod1, ghapx1, and ghcat1) from gossypium hirsutum. overexpressing cassettes containing chimeric ghsod1, ghapx1, or ghcat1 were introduced into cotton plants by agrobacterium transformation, and overexpressed produc ... | 2013 | 23335985 |
| elucidation of nuclear and organellar genomes of gossypium hirsutum: furthering studies of species evolution and applications for crop improvement. | plant genomes are larger and more complex than other eukaryotic organisms, due to small and large duplication events, recombination and subsequent reorganization of the genetic material. commercially important cotton is the result of a polyploidization event between old and new world cottons that occurred over one million years ago. allotetraploid cotton has properties that are dramatically different from its progenitors-most notably, the presence of long, spinnable fibers. recently, the complet ... | 2013 | 24833222 |
| field-acclimated gossypium hirsutum cultivars exhibit genotypic and seasonal differences in photosystem ii thermostability. | previous investigations have demonstrated that photosystem ii (psii) thermostability acclimates to prior exposure to heat and drought, but contrasting results have been reported for cotton (gossypium hirsutum). we hypothesized that psii thermotolerance in g. hirsutum would acclimate to environmental conditions during the growing season and that there would be differences in psii thermotolerance between commercially-available u.s. cultivars. to this end, three cotton cultivars were grown under dr ... | 2013 | 23246028 |