| the complete genomes of lactobacillus plantarum and lactobacillus johnsonii reveal extensive differences in chromosome organization and gene content. | the first comprehensive comparative analysis of lactobacilli was done by comparing the genomes of lactobacillus plantarum (3.3 mb) and lactobacillus johnsonii (2.0 mb). l. johnsonii is predominantly found in the gastrointestinal tract, while l. plantarum is also found on plants and plant-derived material, and is used in a variety of industrial fermentations. the l. plantarum and l. johnsonii chromosomes have only 28 regions with conservation of gene order, totalling about 0.75 mb; these regions ... | 2004 | 15528649 |
| genetic characterization of the bile salt response in lactobacillus plantarum and analysis of responsive promoters in vitro and in situ in the gastrointestinal tract. | in this paper we describe the growth, morphological, and genetic responses of lactobacillus plantarum wcfs1 to bile. growth experiments revealed that a stepwise increase in the porcine bile concentration led to a gradual decrease in the maximal growth rate. moreover, the final density reached by an l. plantarum culture growing in mrs containing 0.1% bile was approximately threefold lower than that in mrs lacking bile. the morphology of the cells grown in mrs containing 0.1% bile was investigated ... | 2004 | 15547253 |
| lactobacillus paraplantarum sp. now., a new species related to lactobacillus plantarum. | four strains of facultatively heterofermentative lactobacilli isolated from beer and human feces have physiological characteristics similar to those of lactobacillus plantarum. unlike 66% of the l. plantarum strains tested (f. bringel, m.-c. curk, and j.-c. hubert, int. j. syst. bacteriol. 46:588-594, 1996), these strains do not catabolize alpha-methyl-d-mannoside. however, because they exhibit little dna relatedness to l. plantarum and lactobacillus pentosus, these four strains were classified ... | 1996 | 15551474 |
| transformation of lactobacillus plantarum by electroporation with in vitro modified plasmid dna. | an improved method for the electrotransformation of lactobacillus plantarum cect 220 (atcc 8014) with plasmid dna isolated from escherichia coli is described. the two main modifications with respect to existing methods are: (i) isolation of plasmid dna from e. coli jm110 grown in minimal medium and (ii) in vitro modification of the dna by cell-free extracts of the host l. plantarum. optimal electrotransformation was obtained with exponentially growing cells of l. plantarum concentrated to 6x10(9 ... | 2004 | 15556712 |
| the possible role of natural idiotopes in immune memory. | in this paper we report on the generation of abs possessing specificities similar to those of abs used in immunization, and on the generation of id and anti-id specificities in the sera of mice immunized with commensal bacterial antigens. the human monoclonal antibody igm dj (vh3/vl2) expresses natural antibody properties, natural idiotope (y7), and specificity towards lactic acid bacteria (lab). when used in immunization it generates lab-specific antibodies. immunization with lab, as detected i ... | 2004 | 15559375 |
| effect of natural starters used for sourdough bread in morocco on phytate biodegradation. | phytase activity was studied in natural sourdough bread starters to determine physicochemical characteristics (phytic acid hydrolysis, dough rising capacity and ph) in the flour and during sourdough fermentation. fermentation microorganisms (yeasts and lactic acid bacteria) were also characterized. results showed a decrease of phytic acid in sourdoughs started with traditional starters, and wide variation in phytase activity. microorganism counts were high at the end of fermentation, indicating ... | 2003 | 15562743 |
| bile-mediated aminoglycoside sensitivity in lactobacillus species likely results from increased membrane permeability attributable to cholic acid. | few studies have been conducted on antimicrobial resistance in lactobacilli, presumably because of their nonpathogenic nature as anaerobic commensals. we assessed resistance in 43 type strains and isolates representing 14 species by using agar disk diffusion and mic analysis in mrs medium. most noteworthy were two general phenotypes displayed by nearly every strain tested: (i) they were more susceptible (up to 256-fold in some cases) to the deconjugated bile acid cholic acid than to the conjugat ... | 2004 | 15574918 |
| simple duplex fecal pcr assay that allows identification of false-negative results in helicobacter sp.-infected mice. | we designed a simple and sensitive duplex polymerase chain reaction (pcr) assay for detection of false-negative results during routine helicobacter sp. feces analysis. we took advantage of the various lactobacillus species that form part of the normal intestinal flora of laboratory rodents to improve our pcr diagnostic assays. using this one-step pcr assay, we were able to rule out false-negative results without the need of adding internal standard molecules. this is an important quality control ... | 2004 | 15575366 |
| water activity affects heat resistance of microorganisms in food powders. | to study the factors and mechanisms involved in microorganisms' death or resistance to temperature in low-water-activity environments, a previous work dealt with the viability of dried microorganisms immobilized in thin-layer on glass beads. this work is intended to check the efficiency of a rapid heating-cooling treatment to destroy microorganisms that were dried after mixing with wheat flour or skim milk. the thermoresistance of the yeast saccharomyces cerevisiae and the bacterium lactobacillu ... | 2005 | 15582741 |
| effect of yeast inoculation rate on the metabolism of contaminating lactobacilli during fermentation of corn mash. | two separate 4 (bacterial concentrations)x6 (yeast concentrations) full factorial experiments were conducted in an attempt to identify a novel approach to minimize the effects caused by bacterial contamination during industrial production of ethanol from corn. lactobacillus plantarum and lactobacillus paracasei, commonly occurring bacterial contaminants in ethanol plants, were used in separate fermentation experiments conducted in duplicate using an industrial strain of saccharomyces cerevisiae, ... | 2004 | 15599666 |
| in vitro screening of potential probiotic activities of selected lactobacilli isolated from unpasteurized milk products for incorporation into soft cheese. | the aim was to select potentially probiotic lactobacilli from 88 strains isolated from unpasteurized milk and cheese products, and to incorporate these bacteria in a viable state into a soft cheese, without changing its quality. the survival of these bacteria was assessed in acidic and bile conditions, after freezing at -80 degrees c. four strains from unpasteurized camembert--two lactobacillus plantarum strains and two lb. paracasei/casei strains--were identified and typed by pcr and pfge and w ... | 2004 | 15605712 |
| succinate production and citrate catabolism by cheddar cheese nonstarter lactobacilli. | to identify strains of cheddar cheese nonstarter lactobacilli that synthesize succinate from common precursors and characterize the biochemical pathways utilized. | 2005 | 15610413 |
| gene organization and sequences of pediocin ach/pa-1 production operons in pediococcus and lactobacillus plasmids. | to determine the locations and sequences of pediocin ach production genes in pediococcus parvulus ato77 from vegetables, lactobacillus plantarum whe92 from muenster cheese, and a lactose-fermenting isolate pediococcus pentosaceus s34 from buffalo milk. | 2005 | 15613003 |
| reduction of non-digestible oligosaccharides in soymilk: application of engineered lactic acid bacteria that produce alpha-galactosidase. | human consumption of soy-derived products has been limited by the presence of non-digestible oligosaccharides (ndo), such as the alpha-galactooligosaccharides raffinose and stachyose. most mammals, including man, lack pancreatic alpha-galactosidase (alpha-gal), which is necessary for the hydrolysis of these sugars. however, such ndo can be fermented by gas-producing microorganisms present in the cecum and large intestine, which in turn can induce flatulence and other gastrointestinal disorders i ... | 2004 | 15614733 |
| phenotypic characterization of lactic acid bacteria from sourdoughs for altamura bread produced in apulia (southern italy). | in order to study the composition of the lactic acid bacteria (lab) community of sourdoughs used for the manufacture of altamura bread, a traditional durum wheat bread produced in apulia (southern italy), 111 strains of lab were isolated and characterized. the phenotypic characterization of the isolates, carried out using a set of 29 tests, allowed the identification of 15 clusters at the 80% similarity level by hierarchical cluster analysis. of the isolates, 88% were identified as facultatively ... | 2005 | 15617801 |
| influence of polysaccharides on oxygen dependent lactate utilization by an amylolytic lactobacillus plantarum strain. | oxygen-dependent conversion of lactate to acetate by the amylolytic strain lactobacillus plantarum a6 was studied using mrs-grown cells, transferred to a basic medium with lactate. in the presence of oxygen, lactate was stoechiometricaly converted to acetate. when glucose, maltose or cellobiose was added to the basic medium, no utilisation of lactate was observed. however, when starch or glycogen was added, the conversion of lactate to acetate happened. to verify the possible link of this effect ... | 2005 | 15617803 |
| production of nitric oxide, tumor necrosis factor-alpha and interleukin-6 by raw264.7 macrophage cells treated with lactic acid bacteria isolated from kimchi. | the present study investigated immunopotentiating activities of lactic acid bacteria isolated from kimchi (klab) in vitro. a raw264.7 macrophage cell line was stimulated with four strains of klab and two strains of bifidobacteria, and then the production of nitric oxide (no) and cytokines--tumor necrosis factor-alpha (tnf-alpha) and interleukin-6 (il-6)--was determined. lactobacillus plantarum, a lactic acid bacteria involved in the latent fermentation of kimchi, was the most effective for the g ... | 2004 | 15630182 |
| high-pressure inactivation of saccharomyces cerevisiae and lactobacillus plantarum at subzero temperatures. | high hydrostatic pressure is a new technology in the food processing industry, and is used for cold pasteurization of food products. however, the pressure inactivation of food-borne microorganisms requires very high pressures (generally more than 400 mpa) and long pressure holding times (5 min or more). carrying out pressure processing at low temperatures without freezing can reduce these parameters, which presently limit the application of this technology, in keeping the quality of fresh raw pr ... | 2004 | 15639102 |
| starch-binding domain affects catalysis in two lactobacillus alpha-amylases. | a new starch-binding domain (sbd) was recently described in alpha-amylases from three lactobacilli (lactobacillus amylovorus, lactobacillus plantarum, and lactobacillus manihotivorans). usually, the sbd is formed by 100 amino acids, but the sbd sequences of the mentioned lactobacillus alpha-amylases consist of almost 500 amino acids that are organized in tandem repeats. the three lactobacillus amylase genes share more than 98% sequence identity. in spite of this identity, the sbd structures seem ... | 2005 | 15640201 |
| probiotication of tomato juice by lactic acid bacteria. | this study was undertaken to determine the suitability of tomato juice as a raw material for production of probiotic juice by four lactic acid bacteria (latobacillus acidophilus la39, lactobacillus plantarum c3, lactobacillus casei a4, and lactobacillus delbrueckii d7). tomato juice was inoculated with a 24-h-old culture and incubated at 30 degrees c. changes in ph, acidity, sugar content, and viable cell counts during fermentation under controlled conditions were measured. the lactic acid cultu ... | 2004 | 15650688 |
| polyphasic study of wine lactobacillus strains: taxonomic implications. | one hundred and seventy-eight lactobacilli isolated from wine were characterized by a polyphasic approach. strains were phenotypically identified at genus and species level by classical tests including the analysis of cell morphology, homo/heterofermentative character, sugar fermentation patterns, growth at different temperatures and the optical nature of the isomer of lactic acid produced from glucose. molecular techniques such as random amplification of polymorphic dna (rapd), amplified 16s rd ... | 2005 | 15653876 |
| detection of acidithiobacillus ferrooxidans in acid mine drainage environments using fluorescent in situ hybridization (fish). | an important microorganism of acid mine drainage (amd) and bioleaching environments is acidithiobacillus ferrooxidans which oxidizes ferrous iron and generates ferric iron, an oxidant. most investigations to understand microbial aspects of sulfide mineral dissolution have focused on understanding physiological, metabolic, and genetic characteristics of a. ferrooxidans. in this study, a 16s rrna oligonucleotide probe designated s-s-t.ferr-0584-a-a-18, and labeled at the 5'-end with indocarbocyani ... | 2005 | 15676194 |
| fermented milk-starch and milk-inulin products as vehicles for lactic acid bacteria. | formulations using cassava starch or inulin plus milk were fermented with three different lactic acid bacteria (lab) strains: lactobacillus plantarum d34, lactobacillus sp. slh6, and streptococcus thermophilus st4. growth and acidification were followed in 3% powdered milk (m3), 3% milk-6% starch (m3-s6), and 3% milk-6% inulin (m3-in6). d34 and slh6 growth was enhanced by starch in m3-s6, when compared to the count (cfu/ml) obtained in m3. growth of all strains was promoted by inulin. all fermen ... | 2004 | 15678724 |
| antibiotic susceptibility profiles of new probiotic lactobacillus and bifidobacterium strains. | the antimicrobial susceptibilities and presence of plasmids in four new probiotic lactic acid bacteria (lab) strains, lactobacillus rhamnosus hn001 (dr20) hn067, lactobacillus acidophilus hn017 and bifidobacterium lactis hn019 (dr10), were determined. resistance to 18 commonly used antibiotics was assessed by disk diffusion. the three lactobacillus strains had similar antibiotic susceptibility profiles to those of lactobacillus plantarum strain hn045 and two commercial probiotic lactobacillus st ... | 2005 | 15681048 |
| intestinal epithelial cells control dendritic cell function. | dendritic cells (dcs) comprise a family of cells specializing in antigen capture and presentation to t cells. we have recently shown that dc play an active role in bacterial uptake across mucosal surfaces. indeed, dc are able to open tight junctions and to sample antigens directly across epithelia, both in vitro and in vivo. because dc express tight junction proteins, the integrity of the epithelial barrier is preserved. in this study we have analyzed the possible involvement of epithelial cells ... | 2004 | 15681745 |
| plasmid p256 from lactobacillus plantarum represents a new type of replicon in lactic acid bacteria, and contains a toxin-antitoxin-like plasmid maintenance system. | lactobacillus plantarum nc7 harbours a single 7.2 kb plasmid called p256. this report describes the complete nucleotide sequence and annotation of p256, as well as the identification of the minimal replicon of the plasmid. based on sequence features in the unusually small (0.7 kb) minimal replicon, and the absence of a gene for a replication-relevant protein, p256 seems to represent a hitherto unknown type of theta replicon in lactic acid bacteria (lab), with a relatively low copy-number. in add ... | 2005 | 15699191 |
| probiotics: wanted dead or alive. | | 2005 | 15702852 |
| survival of the probiotic, l. plantarum 299v and its effects on the faecal bacterial flora, with and without gastric acid inhibition. | probiotic bacteria have to survive passage through the gastrointestinal tract. in this placebo-controlled double-blind study, the effect of lactobacillus plantarum 299v on the faecal flora was studied with and without gastric acid inhibition. | 2005 | 15702859 |
| effect of processing on the antioxidant vitamins and antioxidant capacity of vigna sinensis var. carilla. | cowpea (vigna sinensis l. var. carilla) flours obtained by fermentation with inoculum lactobacillus plantarum (pf) or with the natural microorganisms present in the flour (nf) and subsequent heat treatment in an autoclave were prepared to study the effect of fermentation on the antioxidant vitamin content and on the antioxidant capacity. bacterial counts and ph values, vitamins c and e, carotenoids, glutathione (gsh), superoxide dismutase-like activity (sod-like activity), peroxyl radical-trappi ... | 2005 | 15713044 |
| repression of the pyr operon in lactobacillus plantarum prevents its ability to grow at low carbon dioxide levels. | carbamoyl phosphate is a precursor for both arginine and pyrimidine biosynthesis. in lactobacillus plantarum, carbamoyl phosphate is synthesized from glutamine, atp, and carbon dioxide by two sets of identified genes encoding carbamoyl phosphate synthase (cps). the expression of the carab operon (encoding cps-a) responds to arginine availability, whereas pyraaab (encoding cps-p) is part of the pyrr1bcaaabdfe operon coding for the de novo pyrimidine pathway repressed by exogenous uracil. the pyr ... | 2005 | 15743958 |
| functional analysis of three plasmids from lactobacillus plantarum. | lactobacillus plantarum wcfs1 harbors three plasmids, pwcfs101, pwcfs102, and pwcfs103, with sizes of 1,917, 2,365, and 36,069 bp, respectively. the two smaller plasmids are of unknown function and contain replication genes that are likely to function via the rolling-circle replication mechanism. the host range of the pwcfs101 replicon includes lactobacillus species and lactococcus lactis, while that of the pwcfs102 replicon also includes carnobacterium maltaromaticum and bacillus subtilis. the ... | 2005 | 15746322 |
| overproduction of heterologous mannitol 1-phosphatase: a key factor for engineering mannitol production by lactococcus lactis. | to achieve high mannitol production by lactococcus lactis, the mannitol 1-phosphatase gene of eimeria tenella and the mannitol 1-phosphate dehydrogenase gene mtld of lactobacillus plantarum were cloned in the nisin-dependent l. lactis nice overexpression system. as predicted by a kinetic l. lactis glycolysis model, increase in mannitol 1-phosphate dehydrogenase and mannitol 1-phosphatase activities resulted in increased mannitol production. overexpression of both genes in growing cells resulted ... | 2005 | 15746354 |
| cloning and characterization of the hsp 18.55 gene, a new member of the small heat shock gene family isolated from wine lactobacillus plantarum. | using a molecular approach based on pcr, rt-pcr and northern blot analysis, a new member of the small heat shock family of wine, lactobacillus plantarum, was cloned and characterized. the protein sequence deduced from the isolated gene had a calculated molecular mass of 18.548 kda and was therefore named hsp 18.55. the gene codes for a protein homologous to the previously characterized hsp 19.3 and hsp 18.5 and is co-transcribed with an upstream gene of unknown function. analysis of the 5' flank ... | 2004 | 15748987 |
| a beta-glucosidase gene isolated from wine lactobacillus plantarum is regulated by abiotic stresses. | little genetic information exists on the ability of wine lactic acid bacteria (lab) to hydrolyse glycoconjugates during malolactic fermentation. we tried to fill this important gap by characterizing a gene codifying for a putative beta-glucosidase enzyme from wine lactobacillus plantarum and from a commercial strain of oenococcus oeni. | 2005 | 15752331 |
| lactobacillus hammesii sp. nov., isolated from french sourdough. | twenty morphologically different strains were chosen from french wheat sourdough isolates. cells were gram-positive, non-spore-forming, non-motile rods. the isolates were identified using amplified-fragment length polymorphism, randomly amplified polymorphic dna and 16s rrna gene sequence analysis. all isolates were members of the genus lactobacillus. they were identified as representing lactobacillus plantarum, lactobacillus paralimentarius, lactobacillus sanfranciscensis, lactobacillus spicher ... | 2005 | 15774659 |
| sequence analysis of the lactobacillus plantarum bacteriophage phijl-1. | the complete genomic sequence of a lactobacillus plantarum virulent phage phijl-1 was determined. the phage possesses a linear, double-stranded, dna genome consisting of 36,677 bp with a g+c content of 39.36%. a total of 52 possible open reading frames (orfs) were identified. according to n-terminal amino acid sequencing and bioinformatic analyses, proven or putative functions were assigned to 21 orfs (41%), including 5 structural protein genes. the phijl-1 genome shows functionally related gene ... | 2005 | 15777728 |
| a prospective randomised trial of probiotics in critically ill patients. | probiotics exert a beneficial effect on the host through modulation of gastrointestinal microflora. the aim of this study was to investigate the effect of the probiotic lactobacillus plantarum 299v on gut barrier function and the systemic inflammatory response in critically ill patients. | 2005 | 15784480 |
| the role of val-265 for flavin adenine dinucleotide (fad) binding in pyruvate oxidase: ftir, kinetic, and crystallographic studies on the enzyme variant v265a. | in pyruvate oxidase (pox) from lactobacillus plantarum, valine 265 participates in binding the cofactor fad and is responsible for the strained conformation of its isoalloxazine moiety that is visible in the crystal structure of pox. the contrasting effects of the conservative amino acid exchange v265a on the enzyme's catalytic properties, cofactor affinity, and protein structure were investigated. the most prominent effect of the exchange was observed in the 2.2 a crystal structure of the mutan ... | 2005 | 15794646 |
| use of diet and probiotic therapy in the irritable bowel syndrome: analysis of the literature. | the goal of this report is to review the use of dietary intake and probiotics in patients with irritable bowel syndrome (ibs) in published reports. | 2005 | 15798491 |
| [fermentation of a high-protein plant biomass by introduction of lactic acid bacteria]. | lactic acid bacteria displaying increased ability to produce lactic acid, medium proteolytic activity, and tolerance to osmotic stress were isolated under selective conditions from phyllosphere and rhizosphere of registered and raised cultivars of legumes. lactic fermentation of poorly ensilable leguminous plants (red clover and caucasian goat's rue) was performed by introduction of rifampin-resistant homofermenting representatives of the genus lactobacillus (selected according to a set of techn ... | 2005 | 15810737 |
| characterization of a mobile clpl gene from lactobacillus rhamnosus. | two genes encoding clpl atpase proteins were identified in a probiotic lactobacillus rhamnosus strain, e-97800. sequence analyses revealed that the genes, designated clpl1 and clpl2, share 80% identity. the clpl2 gene showed the highest degree of identity (98.5%) to a clpl gene from lactobacillus plantarum wcfsi, while it was not detected in three other l. rhamnosus strains studied. according to northern analyses, the expression of clpl1 and the clpl2 were induced during heat shock by > 20- and ... | 2005 | 15812039 |
| genome-based in silico detection of putative manganese transport systems in lactobacillus plantarum and their genetic analysis. | manganese serves an important function in lactobacillus plantarum in protection against oxidative stress and this bacterium can accumulate mn(2+) up to millimolar levels intracellularly. although the physiological role of mn(2+) and the uptake of this metal ion have been well documented, the only uptake system described so far for this bacterium is the mn(2+)- and cd(2+)-specific p-type atpase (mnta). recently, the genome of l. plantarum wcfs1 has been sequenced allowing in silico detection of g ... | 2005 | 15817790 |
| cell-surface display of e7 antigen from human papillomavirus type-16 in lactococcus lactis and in lactobacillus plantarum using a new cell-wall anchor from lactobacilli. | the human papillomavirus type-16 (hpv-16) e7 protein is considered a major viral oncoprotein involved in cervical cancer (cxca) and a potential candidate for the development of a vaccine against this neoplasia. here, two lactic acid bacteria (the model one lactococcus lactis and a probiotic one lactobacillus plantarum) were engineered to deliver an e7 mutant protein (e7mm), which has a reduced transforming activity and consequently, could fit better to therapeutic use in humans than the native f ... | 2005 | 15823960 |
| dimanganese catalase--spectroscopic parameters from broken-symmetry density functional theory of the superoxidized mn(iii)/mn(iv) state. | broken-symmetry density functional theory was used to study the catalytic center of manganese catalase in the superoxidized mn(iii)/mn(iv) state. heisenberg exchange coupling constants, 55mn and 14n hyperfine coupling constants (hfcs) and nuclear quadrupole splittings, as well as the electronic g tensors were evaluated for different model systems of the active site after complete geometry optimizations in the high-spin and broken-symmetry states. a comparison of the experimental data with the sp ... | 2005 | 15830216 |
| biodiversity of lactobacillus sanfranciscensis strains isolated from five sourdoughs. | five different sourdoughs were investigated for the composition of lactic acid bacteria (lab) and the biodiversity of lactobacillus sanfranciscensis strains. | 2005 | 15836738 |
| lactobacillus plantarum ccl gene is non-essential, arginine-repressed and codes for a conserved protein in firmicutes. | among proteins specifically found in most gram-positive bacteria of the phylum firmicutes, conserved proteins of the family pfam06177-duf988-cog4708 are of unknown function. the citrulline cluster-linked (ccl) gene of lactobacillus plantarum codes one such protein and is adjacent to the citrulline biosynthesis operon argcjbdf, a situation also found in lactococcus lactis. this gene is well conserved among l. plantarum species, and 1 isolate out of 24 harbored two ccl copies. northern hybridizati ... | 2005 | 15864550 |
| co-cultivation of antifungal lactobacillus plantarum milab 393 and aspergillus nidulans, evaluation of effects on fungal growth and protein expression. | the fungal inhibitory effects of strain lactobacillus plantarum milab 393, producing broad-spectrum antifungal compounds, were evaluated. a co-cultivation method was set up to monitor effects on fungal growth and protein expression of growing aspergillus nidulans with l. plantarum milab 393. the effects of inhibitory metabolites produced by l. plantarum milab 393, cyclo(l-phe-l-pro), lactic acid and 3-phenyllactic acid, were also investigated by addition of pure compounds to the growth medium of ... | 2005 | 15869970 |
| [probiotics as therapeutic agents in irritable bowel syndrome]. | probiotics are defined as living micro-organisms which, when administered in large amounts, confer a health benefit on the host. the use of probiotics in the therapy of infectious bowel diseases as well as maintaining remission of ulcerative colitis and in pouchitis is evidence-based. also, in several studies proof could be supplied that specific probiotics relieve the symptoms and the course of irritable bowel syndrome. some trials showed a significant improvement of irritable bowel syndrome-re ... | 2005 | 15871070 |
| interference of lactobacillus plantarum with pseudomonas aeruginosa in vitro and in infected burns: the potential use of probiotics in wound treatment. | this study evaluated the ability of the probiotic organism lactobacillus plantarum to inhibit the pathogenic activity of pseudomonas aeruginosa, both in vitro and in vivo, and investigated the mechanisms involved in such protection. l. plantarum whole cultures, culture filtrates (acid filtrate and neutralised acid filtrate) and isolated, washed cells were tested in vitro for their effects on the production of the p. aeruginosa quorum-sensing signal molecules, acyl-homoserine-lactones (ahls), and ... | 2005 | 15882197 |
| rapid identification of dairy lactic acid bacteria by m13-generated, rapd-pcr fingerprint databases. | about a thousand lactic acid bacteria (lab) isolated from dairy products, especially cheeses, were identified and typed by species-specific pcr and rapd-pcr, respectively. rapd-pcr profiles, which were obtained by using the m13 sequence as a primer, allowed us to implement a large database of different fingerprints, which were analysed by bionumerics software. cluster analysis of the combined rapd-pcr fingerprinting profiles enabled us to implement a library, which is a collection of library uni ... | 2005 | 15893395 |
| effective probiotic therapy. | | 2005 | 15896436 |
| unity in organisation and regulation of catabolic operons in lactobacillus plantarum, lactococcus lactis and listeria monocytogenes. | global regulatory circuits together with more specific local regulators play a notable role when cells are adapting to environmental changes. lactococcus lactis is a lactic acid bacterium abundant in nature fermenting most mono- and disaccharides. comparative genomics analysis of the operons encoding the proteins and enzymes crucial for catabolism of lactose, maltose and threhalose revealed an obvious unity in operon organisation . the local regulator of each operon was located in a divergent tr ... | 2005 | 15900965 |
| pih01, a small cryptic plasmid from leuconostoc citreum ih3. | a small cryptic plasmid pih01 from leuconostoc citreum ih3 was characterized. this 1.8-kb sized plasmid contains single open reading frame that encodes a repc class protein (342 amino acids) and a conserved pt181-type double strand origin, suggesting a rolling circle replication mode. this putative replicase protein shows the highest similarity to a replicase from pfr18 plasmid of leuconostoc mesenteroides fr52 (64% identity), one of the pt181-type rolling circle plasmid family and contains a st ... | 2005 | 15904957 |
| the effects of various antifungal additives on the fermentation and aerobic stability of corn silage. | in 2 consecutive years, whole plant corn was ensiled in laboratory silos to investigate the effects of various silage additives on fermentation, dry matter (dm) recovery and aerobic stability. in yr 1, chopped forage was treated with 1) no additive (untreated, u), 2) lactobacillus buchneri 40788, 4 x 10(5) cfu/g of fresh forage (llb4), 3) l. buchneri 11a44, 1 x 10(5) cfu/g (plb), 4) biomax 5 (lactobacillus plantarum pa-28 and k-270), 1 x 10(5) cfu/g (b5), 5) silo guard ii (sodium metabisulfite a ... | 2005 | 15905443 |
| metabolic models for rational improvement of lactic acid bacteria as cell factories. | | 2005 | 15916646 |
| effect of additions on ensiling and microbial community of senesced wheat straw. | crop residues collected during or after grain harvest are available once per year and must be stored for extended periods. the combination of air, high moisture, and high microbial loads leads to shrinkage during storage and risk of spontaneous ignition. ensiling is a wet preservation method that could be used to store these residues stably. to economically adapt ensiling to biomass that is harvested after it has senesced, the need for nutrient, moisture, and microbial additions must be determin ... | 2005 | 15917585 |
| cloning, expression, purification, and analysis of mannitol dehydrogenase gene mtlk from lactobacillus brevis. | the commercial production of mannitol involves high-pressure hydrogenation of fructose using a nickel catalyst, a costly process. mannitol can be produced through fermentation by microorganisms. currently, a few lactobacillus strains are used to develop an efficient process for mannitol bioproduction; most of the strains produce mannitol from fructose with other products. an approach toward improving this process would be to genetically engineer lactobacillus strains to increase fructose-to-mann ... | 2005 | 15917616 |
| screening of potential lactobacilli antigenotoxicity by microbial and mammalian cell-based tests. | antigenotoxicity is considered an important property for probiotic lactobacilli. the ability of non probiotic lactobacilli from dairy products and starters to inhibit two reference genotoxins: 4-nitroquinoline-1-oxide and n-methyl-n'-nitro-n-nitrosoguanidine was evaluated. the study was carried out using short-term assays with different targets, such as procaryotic cells (sos-chromotest for genotoxicity in escherichia coli and ames test for mutagenicity in salmonella typhimurium) and eucaryotic ... | 2005 | 15925000 |
| enumeration and identification of pediococci in powder-based products using selective media and rapid pfge. | in this study, pediococci selective medium (psm) was evaluated for the enumeration of pediococcus acidilactici and pediococcus pentosaceus from probiotic animal feed and silage inoculants. psm is based on the complex basal medium mrs supplemented with cysteine hydrochloride, novobiocin, vancomycin, and nystatin. no significant change in electivity was observed when pediococci where recovered from culture or powder-based products following incubation at 37 degrees c under anaerobic conditions for ... | 2006 | 15925418 |
| rapid differentiation and in situ detection of 16 sourdough lactobacillus species by multiplex pcr. | a two-step multiplex pcr-based method was designed for the rapid detection of 16 species of lactobacilli known to be commonly present in sourdough. the first step of multiplex pcr was developed with a mixture of group-specific primers, while the second step included three multiplex pcr assays with a mixture of species-specific primers. primers were derived from sequences that specify the 16s rrna, the 16s-23s rrna intergenic spacer region, and part of the 23s rrna gene. the primer pairs designed ... | 2005 | 15933001 |
| selective probiotic bacteria induce il-10-producing regulatory t cells in vitro by modulating dendritic cell function through dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin. | lactobacilli are probiotic bacteria that are frequently tested in the management of allergic diseases or gastroenteritis. it is hypothesized that these probiotics have immunoregulatory properties and promote mucosal tolerance, which is in part mediated by regulatory t cells (treg cells). on the basis of pathogenic or tissue-specific priming, dendritic cells (dc) acquire different t cell-instructive signals and drive the differentiation of naive t h cells into either t h 1, t h 2, or regulatory e ... | 2005 | 15940144 |
| antimicrobial effects of essential oils in combination with chlorhexidine digluconate. | the aim of the present study was to compare antimicrobial effects of essential oils alone and in combination with chlorhexidine digluconate against planktonic and biofilm cultures of streptococcus mutans and lactobacillus plantarum. the essential oils included cinnamon, tea-tree (melaleuca alternifola), manuka (leptospermum scoparium), leptospermum morrisonii, arnica, eucalyptus, grapefruit, the essential oil mouthrinse cool mint listerine and two of its components, menthol and thymol. cinnamon ... | 2005 | 15943766 |
| addition of formic acid or starter cultures to liquid feed. effect on ph, microflora composition, organic acid concentration and ammonia concentration. | some of the charateristics of good quality fermented liquid feed (flf) are low ph, high numbers of lactic acid bacteria, and low numbers of enterobacteria. in order to test strategies to avoid a proliferation of enterobacteria during the initial phase of flf elaboration, two in vitro studies were carried out. addition of various doses of formic acid or two different starter cultures were tested. adding 0.1% formic acid or l. plantarum vtt e-78076 to the liquid feed seemed to be addecuate ways of ... | 2001 | 15954629 |
| effect of lactobacillus plantarum bacteriocin on gram-negative rods. | the antibacterial effect of bacteriocins synthesized by lactobacillus plantarum isolated from fermented carrot juice on enterobacteriaceae rods coming from food was studied in the experiment. their antibacterial activity was determined employing the diffusion method, well technique. the results show that the bacteriocins examined were characterized by a different effect on test strains whose development was inhibited at the activity level from 256au/ml to 4au/ml. | 2001 | 15954662 |
| use of lactic and propionic acid bacteria in the production of fermented parsley juice. | taking into consideration the proliferation rate of propionibacterium sp. cells, ph changes and sensory properties of the fermented product obtained, selection of propionic acid bacteria (pab) was made in order to determine their usefulness for the production of fermented parsley juice. the analysis included 12 strains, belonging to the following species: p. thoenii (4 strains), p. jensenii (6 strains), p. freudenreichii (1 strain) and p. acidipropionici (1 strain). the experiments show that man ... | 2001 | 15954663 |
| antimicrobial activity of lactic acid bacteria isolated from tenerife cheese: initial characterization of plantaricin tf711, a bacteriocin-like substance produced by lactobacillus plantarum tf711. | the screening and initial characterization of bacteriocins produced by lactic acid bacteria (lab) from raw tenerife goats' cheese with possible application as biopreservatives or ripening accelerators for tenerife cheese. | 2005 | 15960667 |
| a full flora, but not monocolonization by escherichia coli or lactobacilli, supports tolerogenic processing of a fed antigen. | fed protein undergoes processing and coupling to major histocompatibility complex (mhc) ii molecules during passage through the intestinal epithelium, generating a tolerogenic form of the antigen in serum. transfer of this factor to naïve animals induces tolerance in the recipient. in this study, we investigate what impact colonization with gram-positive (lactobacillus plantarum) or gram-negative (escherichia coli) bacteria has on tolerogenic processing in the gut. germ-free (gf), monocolonized ... | 2005 | 15963047 |
| development of methods for the detection and quantification of 7alpha-dehydroxylating clostridia, desulfovibrio vulgaris, methanobrevibacter smithii, and lactobacillus plantarum in human feces. | mounting evidence suggests a relationship between bacterial metabolism of certain dietary and endogenous factors and the development of colorectal cancer. deoxycholic acid (dca) is a well studied co-carcinogen and bio-transformation product of 7alpha-dehydroxylating clostridia. h2s is a cytotoxic metabolite produced primarily by sulfate-reducing bacteria (srb). the production of methane indicates low levels of active srb. lactic acid bacteria (lab) have received attention recently due to their p ... | 2005 | 15963794 |
| characterization of lactobacillus strains and monitoring by rapd-pcr in controlled fermentations of "almagro" eggplants. | the characterization of 23 lactobacillus strains was performed. the strains were assayed for biogenic amine-forming capacity, hydrogen peroxide production, pectin esterase, cellulase and polygalacturonase production, growth rate, acidifying capacity and salt tolerance. three strains were selected which belonged to the species, lactobacillus brevis, lactobacillus plantarum and lactobacillus fermentum. different starter cultures prepared as combinations of these three strains were assayed in pilot ... | 2005 | 15979183 |
| growth of lactic acid bacteria and rhizopus oligosporus during barley tempeh fermentation. | the zygomycete rhizopus oligosporus is traditionally used to ferment soybean tempeh, but it is also possible to ferment other legumes and cereals to tempeh. the traditional soybean tempeh harbours a multitude of microorganisms with potentially beneficial or detrimental effects on quality. lactic acid bacteria (lab) have positive effects on the safety of soybean tempeh, but the effects of lab on r. oligosporus growth have not been investigated. we have developed a cereal grain tempeh by fermentin ... | 2005 | 15979185 |
| quenching of microbial samples for increased reliability of microarray data. | messenger rna levels change on a minutes scale due to both degradation and de novo transcription. consequently, alterations in the transcript profiles that are not representative for the condition of interest are easily introduced during sample harvesting and work-up. in order to avoid these unwanted changes we have validated a -45 degrees c methanol-based quenching method for obtaining reliable and reproducible 'snapshot' samples of lactobacillus plantarum cells for transcriptome analyses. tran ... | 2006 | 15982764 |
| enhanced antiinflammatory capacity of a lactobacillus plantarum mutant synthesizing modified teichoic acids. | teichoic acids (tas), and especially lipoteichoic acids (ltas), are one of the main immunostimulatory components of pathogenic gram-positive bacteria. their contribution to the immunomodulatory properties of commensal bacteria and especially of lactic acid bacteria has not yet been investigated in detail. to evaluate the role of tas in the interaction between lactic acid bacteria and the immune system, we analyzed the antiinflammatory properties of a mutant of lactobacillus plantarum ncimb8826 a ... | 2005 | 15985548 |
| adhesion of the probiotic bacterium lactobacillus plantarum 299v onto the gut mucosa in critically ill patients: a randomised open trial. | to achieve any possible positive effect on the intestinal mucosa cells it is important that probiotics adhere tightly onto the intestinal mucosa. it has been shown in healthy volunteers that lactobacillus plantarum 299v (lp 299v) (dsm 9843), a probiotic bacterium, given orally in a fermented oatmeal formula adheres onto the intestinal mucosa, but whether this also occurs in critically ill patients is unknown. | 2005 | 15987403 |
| direct regulatory immune activity of lactic acid bacteria on der p 1-pulsed dendritic cells from allergic patients. | lactic acid bacteria (lab) are suggested to play a regulatory role in the development of allergic reactions. however, their potential effects on dendritic cells (dcs) directing the immune polarization remain unclear. | 2005 | 15990795 |
| high-level, inducible gene expression in lactobacillus sakei and lactobacillus plantarum using versatile expression vectors. | vectors have been developed for inducible gene expression in lactobacillus sakei and lactobacillus plantarum in which expression of the gene of interest is driven by strong, regulated promoters from bacteriocin operons found in l. sakei strains. the activity of these promoters is controlled via a two-component signal transduction system, which responds to an externally added peptide pheromone. the vectors have a modular design, permitting easy exchange of all essential elements: the inducible pr ... | 2005 | 16000734 |
| lactobacillus plantarum subsp. argentoratensis subsp. nov., isolated from vegetable matrices. | fourteen strains isolated from vegetable sources and identified as belonging to lactobacillus plantarum presented an atypical pattern of amplification with a species-specific multiplex-pcr assay. phylogenetic analysis of two protein-encoding genes, reca (encoding the recombinase a protein) and cpn60 (encoding the groel chaperonin), as well as phenotypic and genomic traits revealed a homogeneous group of very closely related strains for which subspecies status is proposed, with the name lactobaci ... | 2005 | 16014493 |
| characterization of bacteriocins produced by lactic acid bacteria isolated from spoiled black olives. | bacteriocin-producing strains of lactobacillus plantarum st23ld and st341ld, enterococcus faecium st311ld and leuconostoc mesenteroides subsp. mesenteroides st33ld were isolated from the brine of spoiled black olives. the bacteriocins produced by all four strains inhibited the growth of gram-positive bacteria (e. faecalis, l. casei and streptococcus pneumoniae), but also escherichia coli and pseudomonas aeruginosa. strain st23ld produced two bacteriocins (st23lda and st23ldb of approximately 3.0 ... | 2005 | 16028203 |
| pantothenic acid quantification: method comparison of a stable isotope dilution assay and a microbiological assay. | different foods and feedstuffs were analyzed for pantothenic acid (pa) by the recently developed stable isotope dilution assay (sida) and by the standard method, a microbiological assay (ma). the sida involved the use of [13c3, 15n]-pantothenic acid as the internal standard and detection by liquid chromatography-tandem mass spectrometry. the analysis of identical extracts minimized systematic bias due to equal extraction yields and enabled an ideal comparison between both methods. for the sample ... | 2005 | 16028638 |
| an agr-like two-component regulatory system in lactobacillus plantarum is involved in production of a novel cyclic peptide and regulation of adherence. | we have analyzed a locus on the annotated lactobacillus plantarum wcfs1 genome that showed homology to the staphylococcal agr quorum-sensing system and designated it lam for lactobacillus agr-like module. production of the lambdca transcript was shown to be growth phase dependent. analysis of a response regulator-defective mutant (delta)lama) in an adherence assay showed that lam regulates adherence of l. plantarum to a glass surface. global transcription analysis of the wild-type and (delta)lam ... | 2005 | 16030216 |
| lipotechoic acid in lactobacilli: d-alanine makes the difference. | | 2005 | 16046536 |
| nucleotide sequence, structural organization and host range of prs4, a small cryptic pediococcus pentosaceus plasmid that contains two cassettes commonly found in other lactic acid bacteria. | the complete nucleotide sequence of the cryptic plasmid prs4 (3550 bp) from pediococcus pentosaceus rs4 was determined. sequence analysis revealed the presence of three open reading frames (orfs). the putative protein coded by orf 1 showed 93% identity with the mobilization protein of lactobacillus casei plasmid plc88 and 94% identity with a sequenced fragment of the mobilization protein of p. damnosus plasmid pf8801, suggesting a common origin for these three mobilization proteins. the putative ... | 2005 | 16054305 |
| identification of predominant lactic acid bacteria isolated from traditionally fermented vegetable products of the eastern himalayas. | gundruk, sinki and khalpi are lactic-fermented vegetable products of sikkim in india, and inziangsang is a fermented leafy vegetable product of nagaland and manipur in india. a total of 65 samples of gundruk (25), sinki (12), khalpi (25) and inziangsang (3) were analysed for microbial counts. the population of lactic acid bacteria (lab) as well as aerobic mesophilic counts were at the level of 10(7) cfu g(-1). yeasts were detected only in few samples of sinki and khalpi. no moulds were detected. ... | 2005 | 16055218 |
| identification of potential probiotic starter cultures for scandinavian-type fermented sausages. | potential probiotic cultures suitable as starter cultures for the scandinavian-type fermented sausages were identified among strains well-adapted to fermented meats as well as strains originating from a culture collection. from 15 different fermented meat products, 22 strains were isolated as dominant non-starter lactic acid bacteria (nslab). the isolates were identified by rapd, api and sequence analysis of 16s rrna and showed to be five strains of lactobacillus sakei, five strains of lactobaci ... | 2005 | 16076509 |
| monitoring the bacterial population dynamics during fermentation of artisanal argentinean sausages. | the dynamics of the microbial community responsible for the artisanal fermentation of dry sausage produced in argentina was investigated by using classical and molecular approaches. the combined use of rapd analysis with primers m13, xd9, rapd1 and rapd2 and 16s rdna sequencing were applied to the identification and intraspecific differentiation of 100 strains of lactobacilli and micrococcaceae. dgge analysis was used to monitor the dynamic changes in population after total microbial dna was dir ... | 2005 | 16083816 |
| susceptibility to some antifungal drugs of vaginal lactobacilli isolated from healthy women. | samples were taken from the lateral vaginal wall of 19 healthy women. lactobacilli were isolated from ten of these women. fifty-eight lactobacillus isolates were identified as l. gasseri, l. vaginalis, l. acidophilus, l. delbrueckii spp. lactis, l. crispatus, l. plantarum, l. cellobiosus, l. jensenii, l. salivarius, l. curvatus, l. brevis and l. oris. the inhibitory effect of isoconazole and oxiconazole in 0.2, 0.1 and 0.05 microg/ml concentrations on 58 lactobacillus spp. and candida albicans ( ... | 2005 | 16086557 |
| the assessment of biological activities associated with the major constituents of the methanol extract of 'wild carrot' (daucus carota l) seeds. | daucus carota l. (family: apiaceae alt. umbelliferae), commonly known as 'wild carrot' or 'queen anne's-lace,' is an ecologically invasive erect biennial naturalized to scotland. the ethnobotanical uses of this species include applications in the treatment of cough, diarrhea, dysentery, cancer, malaria and tumors, and as an antiseptic, abortifacient, aphrodisiac, carminative, stimulant, stomachic and tonic. the major constituents isolated from the methanol extract of d. carota seeds by reversed- ... | 2005 | 16093236 |
| diversity and technological properties of predominant lactic acid bacteria from fermented cassava used for the preparation of gari, a traditional african food. | traditional fermentation of cassava is dominated by a lactic acid bacteria (lab) population. fermentation is important for improving product flavour and aroma as well as safety, especially by reduction of its toxic cyanogenic glucosides. the production of gari from cassava in benin typically occurs on a household or small industrial scale, and consequently suffers from inconsistent product quality and may not always be safe for consumption. therefore, the diversity of lab from a typical cassava ... | 2005 | 16104351 |
| in situ activity of a bacteriocin-producing lactococcus lactis strain. influence on the interactions between lactic acid bacteria during sourdough fermentation. | to biochemically characterize the bacteriocin produced by lactococcus lactis ssp. lactis m30 and demonstrate its effect on lactic acid bacteria (lab) during sourdough propagation. | 2005 | 16108809 |
| exploring lactobacillus plantarum genome diversity by using microarrays. | lactobacillus plantarum is a versatile and flexible species that is encountered in a variety of niches and can utilize a broad range of fermentable carbon sources. to assess if this versatility is linked to a variable gene pool, microarrays containing a subset of small genomic fragments of l. plantarum strain wcfs1 were used to perform stringent genotyping of 20 strains of l. plantarum from various sources. the gene categories with the most genes conserved in all strains were those involved in b ... | 2005 | 16109953 |
| biodiversity-based identification and functional characterization of the mannose-specific adhesin of lactobacillus plantarum. | lactobacillus plantarum is a frequently encountered inhabitant of the human intestinal tract, and some strains are marketed as probiotics. their ability to adhere to mannose residues is a potentially interesting characteristic with regard to proposed probiotic features such as colonization of the intestinal surface and competitive exclusion of pathogens. in this study, the variable capacity of 14 l. plantarum strains to agglutinate saccharomyces cerevisiae in a mannose-specific manner was determ ... | 2005 | 16109954 |
| compared tolerance to osmotic stress in various microorganisms: towards a survival prediction test. | the osmotic tolerance of microbial cells of different microorganisms was investigated as a function of glycerol concentration and temperatures. cells displayed specific sensitivity to dehydration in glycerol solutions. the viability of gram-negative strains (escherichia coli, bradyrhizobium japonicum), gram-positive strains (lactobacillus plantarum, l. bulgaricus), and yeasts (saccharomyces cerevisiae, candida utilis) decreased with increasing osmotic pressure. for each strain, a characteristic ... | 2005 | 16116658 |
| production of probiotic cabbage juice by lactic acid bacteria. | research was undertaken to determine the suitability of cabbage as a raw material for production of probiotic cabbage juice by lactic acid bacteria (lactobacillus plantarum c3, lactobacillus casei a4, and lactobacillus delbrueckii d7). cabbage juice was inoculated with a 24-h-old lactic culture and incubated at 30 degrees c. changes in ph, acidity, sugar content, and viable cell counts during fermentation under controlled conditions were monitored. l. casei, l. delbrueckii, and l. plantarum grew ... | 2006 | 16125381 |
| construction of a new reporter system to study the nacl-dependent dnak promoter activity of lactobacillus sanfranciscensis. | a reporter system was developed to study gene expression in lactobacillus sanfranciscensis. it was based on the escherichia coli/lactobacillus shuttle vector plp3537 and the mela gene encoding alpha-galactosidase originating from lactobacillus plantarum. mela was functionally expressed in e. coli and l. sanfranciscensis, and activity was easily monitored in vivo as well as in vitro by applying an optimized enzyme assay. the reporter system was validated by demonstrating the induction of the dnak ... | 2006 | 16133335 |
| lactic acid bacteria and yeasts associated with spontaneous fermentations during the production of sour cassava starch in brazil. | sour cassava starch is a traditional fermented food used in the preparation of fried foods and baked goods such as traditional cheese breads in brazil. thirty samples of sour cassava starch were collected from two factories in the state of minas gerais. the samples were examined for the presence of lactic acid bacteria, yeasts, mesophilic microorganisms, bacillus cereus and faecal coliforms. lactic acid bacteria and yeasts isolates were identified by biochemical tests, and the identities were co ... | 2005 | 16153731 |
| survival, growth and persistence under farm conditions of a lactobacillus plantarum strain inoculated into liquid pig feed. | to investigate the survival and persistence of lactobacillus plantarum reb1 in the fermentation process of liquid pig feed on a working farm in standard production conditions. | 2005 | 16162236 |
| lactate racemization as a rescue pathway for supplying d-lactate to the cell wall biosynthesis machinery in lactobacillus plantarum. | lactobacillus plantarum is a lactic acid bacterium that produces d- and l-lactate using stereospecific nad-dependent lactate dehydrogenases (ldhd and ldhl, respectively). however, reduction of glycolytic pyruvate by ldhd is not the only pathway for d-lactate production since a mutant defective in this activity still produces both lactate isomers (t. ferain, j. n. hobbs, jr., j. richardson, n. bernard, d. garmyn, p. hols, n. e. allen, and j. delcour, j. bacteriol. 178:5431-5437, 1996). production ... | 2005 | 16166538 |
| biodiversity of lactic acid bacteria in moroccan soft white cheese (jben). | the bacterial diversity occurring in traditional moroccan soft white cheese, produced in eight different regions in morocco, was studied. a total of 164 lactic acid bacteria were isolated, purified and identified by whole-cell protein fingerprinting and rep-pcr genomic fingerprinting. the majority of the strains belonged to the genera lactobacillus, lactococcus, leuconostoc and enterococcus. sixteen species were identified: lactobacillus plantarum, lactobacillus rhamnosus, lactobacillus paracase ... | 2005 | 16168579 |
| oral immunization of mice with lactic acid bacteria producing helicobacter pylori urease b subunit partially protects against challenge with helicobacter felis. | the development of an efficacious vaccine against infection with helicobacter pylori, the causative agent of chronic gastritis, peptic ulcer disease, and gastric adenocarcinoma, remains a challenge. since the use of mucosal adjuvants is limited in human application, we have evaluated the potential of recombinant lactobacillus strains producing h. pylori urease b (ureb) subunit to deliver this antigen to the gastrointestinal tract. | 2005 | 16170763 |
| the effects of lactobacillus-fermented milk on lipid metabolism in hamsters fed on high-cholesterol diet. | the objective of this study was to evaluate the effects of local lactobacillus strains (ntu 101 and 102) on cholesterol-lowering effects in vivo. thirty male hamsters were housed, divided into five groups, and fed on a cholesterol diet (5 g/kg diet) to induce hypercholesterolemia. milk fermented by lactobacillus paracasei subsp. paracasei ntu 101, lactobacillus plantarum ntu 102, and lactobacillus acidophilus bcrc 17010 was administrated for this study. after treatment with different fermented m ... | 2006 | 16172889 |
| resistance of lactobacillus plantarum kctc 3099 from kimchi to oxidative stress. | the antioxidative capacity of two lactic acid bacteria isolated from kimchi, a korean fermented food, was evaluated by measuring the resistance to reactive oxygen species (ros) and compared with that of lactobacillus rhamnosus gg as a positive control. both intact cells and cell-free extracts of lactobacillus plantarum kctc 3099 exhibited higher antioxidative activity in inhibiting lipid peroxidation among the strains evaluated with an inhibitory level of 38.6% and 48.5%, respectively. to evalua ... | 2005 | 16176138 |