| [studies on lipids of spore-forming bacteria. ii. changes in fatty acid composition of bacillus subtilis cells during growth and sporulation (author's transl)]. | | 1977 | 410967 |
| [studies on lipids of spore-forming bacteria. iii. composition of lipids in vegetative cells and spores of bacillus subtilis (author's transl)]. | | 1977 | 410968 |
| tissue distribution and residues of aminoglycoside antibiotics in normal dairy cows. | the concentration and persistence of streptomycin, dihydostreptomycin (dhs), neomycin, and kanamycin in the body organs of normal dairy cows following intravenous (i.v.) and intramuscular (i.m.) injections were determined by qualitative and quantitative assay methods. the most sensitive qualitative procedure, i.e. bacillus subtilis bga at ph 8.0, failed to detect kanamycin in the meat of cows slaughtered 4 hours after i.m. and 6 hours after i.v. injections. this test, however, was positive for t ... | 1977 | 411195 |
| a new assay for transaminase c. | | 1977 | 411384 |
| purification of cohesive-end-producing restriction endonuclease from bacillus subtilis g. | a new restriction endonuclease was partially purified from bacillus subtilis g (iam1247). this restriction endonuclease (endonuclease rbsug) seems to produce cohesive ends at its cleavage site. | 1977 | 411515 |
| effects of carrier morphology and buffer diffusion on the expression of enzymatic activity. | a very stable esterase (ec 3.1.1.-), which hydrolyses ethyl acetate, cephalosporin c and other acetyl esters with a maximum turnover number of 3-10(2) s-1, was isolated from bacillus subtilis atcc 6633 and immobilized on two supports: controlled-pore glass and powdered brick, a representative of carriers having a wide pore-size distribution. carrier morphology determines diffusion rates and the expression of activity. rate-limiting mass transfer of buffer leads to apparent losses of activity, gr ... | 1977 | 411518 |
| [isolation and some properties of phospholipase d from bacillus subtilis g-22]. | a method of isolating highly purified phospholipase d from bac. subtilis g-22 is described. it includes ammonium sulphate fractionation, thermal denaturation, chromatography on lipoprotein bound with sepharose 6b and ah-sepharose 4b. the enzyme is 130-fold purified, its yield exceeds 90.0%, its specific activity is 164 units per mg of protein. the homogeneity of the enzyme is demonstrated by polyacrylamide gel electrophoresis, ultracentrifugation, isoelectric focusing and n-terminal amino acid d ... | 1977 | 411526 |
| the effect of waring blendor treatment on transformation in bacillus subtilis. | treatment of competent bacillus subtilis in a waring blendor for 10 s increases transformability of the culture about twofold while reducing the attachment of dna to competent cells by 80%. the effectiveness of attached dna in producing transformants is increased 10-fold by this treatment. the uptake of transforming dna into a dnase-resistant state is progressively reduced by 70% during a 120-s blending treatment. blending for 30-45 s diminishes transformability to about 10% of the original nonb ... | 1977 | 411563 |
| serum gentamicin assay by high-performance liquid chromatography. | we describe a high-performance liquid chromatographic method for the quantitative determination of gentamicin in serum. the antibiotic was separated from serum by passage through a silicic acid column, derivatized with o-phthalaldehyde, and eluted with ethanol. the derivatized gentamicin was then separated into all three of its major components by reversed-phase chromatography and quantified by fluorometry. concentrations in serum as low as 0.5 mg of gentamicin per liter could be accurately dete ... | 1977 | 411610 |
| metabolic disposition of cephalothin and deacetylcephalothin in children and adults: comparison of high-performance liquid chromatographic and microbial assay procedures. | | 1977 | 411623 |
| the distribution of lipids in the protoplast membranes of bacillus subtilis. a study with phospholipase c and trinitrobenzenesulphonic acid. | | 1977 | 411655 |
| the production of bacitracin synthetase by bacillus licheniformis atcc 10716. | | 1977 | 411685 |
| the influence of the developing bacterial spore on the mother cell. | | 1977 | 411705 |
| interaction of granaticin b with the transcription system of bacillus subtilis. | the interaction of granaticin b, a quinone antibiotic produced by streptomyces granaticolor, with some biologically important bivalent metal ions, dna and atp was demonstrated spectrophotometrically. the activity of isolated rna polymerase was higher when the dna of phage sp 50 served as template than with dna isolated from bacillus subtilis. granaticin b inhibited in vitro rna synthesis, similarly to certain other antibiotics (the inhibition was three times lower than that caused by actinomycin ... | 1977 | 411719 |
| transformation in bacillus subtilis. initial stages of the transformation process and their energy dependence. | nan3 was found to inhibit transformation but not the irreversible binding of donor 3h-dna in competent cells of the original low-transformable strain bacillus subtilis 168 trp2. addition of nan3 to cells of two mutants bacillus subtilis ht39 and ht46 with an increased transformability decreased substantially the irreversible binding of the donor dna to the competent cells. the decreased irreversible binding of dna is caused by an increased osmotic sensitivity of competent cells of the mutants ht ... | 1977 | 411720 |
| kuwaitimycin, effect on synthesis of lipids in bacillus subtillis cells. | the effects exerted by kuwaitimycin on synthesis of lipids as well as some metabolic activities of bacillus subtilis were studied. the antibiotic not only arrested the inocrporation of 14c-acetate into the microbial lipids but also altered the fatty acids pattern, contents of i-c 15, a-c 15, i-c 17 and a-c 17 were markedly reduced, concomitant with an increase in the contents of i-c 14 and n-c 14. moreover, the rates of synthesis of phospholipids were decreased by the drug, especially that of p ... | 1977 | 411777 |
| restriction-like phenomena in transformation of bacillus subtilis reca. | genetic transformation in reca1 strains of bacillus subtilis was studied to test the hypothesis that, in these strains, a major pathway of recombination is missing, leaving only residual transformation via a pathway specific for transduction. the two putative recombinational pathways have been hypothesized to differ in either length of synapsed regions or specificity for nucleotide sequence homology. it was found that the efficiency of transformation of reca1 cells by deoxyribonucleic acid (dna) ... | 1977 | 411782 |
| repair and subsequent fragmentation of deoxyribonucleic acid in ultraviolet-irradiated bacillus subtilis reca. | cells of bacillus subtilis reca1 are sensitive to irradiation with ultraviolet light. evidence is presented here that these cells are not defective in ultraviolet light-induced incision of deoxyribonucleic acid (dna) or repair dna synthesis. ligation of dna at repair sites appears to occur, but the dna is subsequently fragmented, apparently at sites of previous repair synthesis. it is hypothesized that the defect in dna repair leads to host-specific restriction at repaired sites because of a def ... | 1977 | 411783 |
| reconstitution studies show that rifampicin resistance is determined by the largest polypeptide of bacillus subtilis rna polymerase. | a procedure has been developed to separate the subunits of bacillus subtilis rna polymerase rapidly and in good yield. the method involved the use of a blue dextran-sepharose column which bound the beta' subunit. a phosphocellulose column was used to separate the alpha and beta subunits. during purification, the enzyme eluted from the dna-cellulose column in three separate forms in the order alpha2betabeta'deltaomega1,alpha2betabeta'omega1, and alpha2betabeta'omega1sigma. subunit reconstitution ... | 1977 | 411792 |
| inactivation of bacillus spores in dry systems at low and high temperatures. | a plot of the thermal resistance of bacillus subtilis var. niger spores (log d value) against temperature was linear between 37 and 190 degrees c (z = 23 degrees c), provided that the relative humidity of the spore environment was kept below a certain critical level. the corresponding plot for bacillus stearothermophilus spores was linear in the range 150 to 180 degrees c (z = 29 degrees c) but departed from linearity at lower temperatures (decreasing z value). however, the z value of 29 degrees ... | 1977 | 411885 |
| morphological stages of bacillus subtilis sporulation and resistance to fusidic acid. | during spore development of bacillus subtilis both protein synthesis and sporulation become resistant to the antibiotic fusidic acid. this resistance develops at the time when asymmetric prespore septa are formed. simultaneously ribosomes lose their ability to bind fusidic acid, as demonstrated by their affinity chromatography with the immobilized drug. mutants resistant to fusidic acid during growth are oligosporogenous; their sporulation development is blocked before septum formation. these re ... | 1977 | 411887 |
| inhibitors of bacillus subtilis dna polymerase iii. structure-activity relationships of 6-(phenylhydrazino)uracils. | 6-(phenylhydrazino)uracils inhibit the replication-specific enzyme dna polymerase iii of bacillus subtilis by forming a strong, reversible complex with template-primer dna and enzyme. the phenyl ring interacts with a hydrophobic enzyme site which, on the basis of structure-activity relationships of substituted analogues, appears to possess the following characteristics: (1) planarity or near-planarity; (2) a finite capacity to accommodate bulky substituents; and (3) location near the domain of t ... | 1977 | 411933 |
| inhibitors of bacillus subtilis dna polymerase iii. 6-(arylalkylamino)uracils and 6-anilinouracils. | 6-(benzylamino)uracils and substituted 6-anilinouracils have been found to be potent inhibitors of bacillus subtilis dna polymerase iii by a mechanism identical with that of 6-(phenylhydrazino)uracils. higher phenylalkylamino homologues are progressively weaker inhibitors of the enzyme. examination of the effects of substituents on the activity of 6-(benzylamino)uracils against wild-type and mutant enzymes and preliminary results for 6-anilinouracils have permitted further dissection of the mech ... | 1977 | 411934 |
| selective enrichment for genetic markers in dna released by competent cultures of bacillus subtilis. | deoxyribonucleic acid is released into the growth medium by bacillus subtilis at the time of competence. this dna is enriched for the genetic markers which have previously been demonstrated to be elevated in membrane-dna preparations and more recently in cell wall-dna complexes. furthermore, the pura16/leu-8 relative marker enrichment varies with time, reaching its highest point at the time of maximal competence. enrichment remains elevated for at least 60 min further in the competence regimen. ... | 1977 | 412055 |
| purification of a positive regulatory subunit from phage sp01-modified rna polymerase. | a phage-induced subunit has been purified from phage sp01-modified bacillus subtilis rna polymerase. this subunit binds in vitro to rna polymerase core from uninfected b. subtilis thereby template-selective transcription and asymmetric synthesis of sp01 middle rna. | 1977 | 412108 |
| calcium ion regulates chemotactic behaviour in bacteria. | | 1977 | 412110 |
| sensory electrophysiology of bacteria: relationship of the membrane potential to motility and chemotaxis in bacillus subtilis. | the relationship of membrane potential to motility and chemotaxis of bacillus subtilis has been tested by using the fluorescence of a cyanine dye as a probe of the potential. the dye fluorescence was found to be an indicator of membrane potential by correlation with triphenylmethylphosphonium ion distribution and with changes due to anaerobicity and ionophore addition. when the potential was sufficient for motility and constant over time, it was found that the absolute level of the potential did ... | 1977 | 412194 |
| ["host-controlled modification and restriction" and the relating enzymes in bacillus subtilis and other bacillus strains (author's transl)]. | | 1977 | 412233 |
| [molecular breeding in e. coli, and newly found bacillus subtilis plasmids (author's transl)]. | | 1977 | 412234 |
| characterization of bacterial growth by means of flow microfluorometry. | by means of flow microfluorometry, the protein and nucleic acid contents of individual bacterial cells may be measured at the rate of several thousand cells per second. accumulation of such information over a few minutes yields the composition distribution of the microbial population. these distributions have been determined at different times during batch growth of bactillus subtilis, and the results indicate that the variance of cell composition decreases as the population passes through the e ... | 1977 | 412254 |
| [suppression of mutations arising during transformation of bacillus subtilis cells using foreign dna]. | | 1977 | 412287 |
| studies of bacillus subtilis phage m2: a physical map of the m2 genome. | | 1977 | 412316 |
| restriction fragment analysis of the temporal program of bacteriophage spo1 transcription and its control by phage-modified rna polymerases. | | 1977 | 412317 |
| simple and rapid colorimetric method for the microdetermination of alpha amylase. | | 1977 | 412348 |
| purification and partial characterization of the gastric ulcer inhibitory substance from culture filtrate of bacillus subtilis h. | | 1977 | 412598 |
| attachment of carbohydrate to enzymes increases their circulatory lifetimes. | | 1977 | 412706 |
| ribosomal dimers produced by dimers of aurintricarboxylic acid. | | 1977 | 412707 |
| inhibition of phenylalanine trna synthetase from bacillus subtilis by ochratoxin a. | | 1977 | 412710 |
| transformation of bacillus subtilis and escherichia coli by a hybrid plasmid pcd1. | the gene thyp3 from bacillus subtilis bacteriophage phi 3t was cloned in the plasmid pmb9. the resulting chimeric plasmid, pcd1, is effective in transforming both escherichia coli and bacillus subtilis to thymine prototrophy. the activity of the thyp3 gene product, thymidylate synthetase, was assayed and found to be 9 times greater in a transformed strain of escherichia coli than in a phi 3t lysogen of bacillus subtilis. the physical location of restriction sites has been determined for two rela ... | 1977 | 412725 |
| effect of site-specific endonuclease digestion on the thyp3 gene of bacteriophage phi 3t and the thyp11 gene of bacteriophage rho11. | phi 3t and rho11 are closely related bacteriophages of bacillus subtilis which can "convent" thymine auxotrophs to thymine prototrophs upon infection or transfection. the effect of endonuclease digestion on the ability of both bacteriophage and prophage dna from phi et and rho11 to transform for thymine prototrophy was determined. all of the endonucleases tested: bamhi, bg/ii, bsuri, ecori, hindii+ iii, and hpaii reduced the efficiency of thyp transformation to an equal extent in prophage and ba ... | 1977 | 412726 |
| studies on the ionophorous antibiotics. xi. the artifacts and the degradation products of lysocellin. | lysocellin is a new polyether antibiotic produced by streptomyces cacaoi var. asoensis and is characterized as a broad spectrum ionophore having a higher complexation affinity for divalent cations than for monovalents and also having an ability to transport biological amines. the structures of two artifacts designated l1 and m1 have been elucidated based on spectral evidence, and the formation mechanism of these compounds was discussed with respect to the reactivity of the antibiotic. in additio ... | 1977 | 412819 |
| relationship of ribosomal binding and antibacterial properties of tylosin-type antibiotics. | | 1977 | 412822 |
| genetics of the alpha-ketoglutarate dehydrogenase complex of bacillus subtilis. | the enzymatic defects in a number of bacillus subtilis mutants of the alpha-ketoglutarate dehydrogenase complex lacking activity have been investigated. mutants in the citk locus, as well as a series of deletions of unknown length covering the citk locus, are deficient in e1 of the complex, alpha-ketoglutarate dehydrogenase, but have normal activities of e2, dehydrolipoyl transsuccinylase, and e3, lipoamide dehydrogenase. the citk mutants and the citl22 mutant show in vitro complementation of al ... | 1978 | 412834 |
| temperature-sensitive nature of the rodb maturation in bacillus subtilis. | the mg2+ requirement of a morphological mutant of bacillus subtlis, rodb strain 104 was highly temperature sensitive in the presence of halide or nitrate anions. likewise the morphological change from rod shapes to spheres was dependent upon temperature, the same anions, and the mg2+ concentration. the three factors interacted. other rodb mutants behaved similarly. if the rodb strain 104 in its rod form was treated at high temperatures in the absence of either protein or peptidoglycan synthesis ... | 1978 | 412835 |
| cell polarity in bacillus subtilis: effect of growth conditions on spore positions in sister cells. | | 1977 | 412916 |
| the shape of the ultraviolet inactivation curve for transforming dna. | | 1977 | 413044 |
| [certain data on the protoplast ultrastructure]. | a study was made of the structure of bac. subtilis and listeria monocytogenes protoplasts by the method of scanning electron microscopy. the mechanism of protoplast formation in gram-positive bacteria and in spheroplasts of gram-negative bacteria proved to differ. a loss of the rigid form of the cell, round protrusions on cell surface, and an escape of the cytoplasm through the ruptured cell wall in some one place was noted in case of protoplasts. individual cells can coalesce with one another w ... | 1977 | 413293 |
| pholipomycin, a new member of phosphoglycolipid antibiotics. iii. biological properties. | pholipomycin, a new member of the phosphoglycolipid antibiotics, was primarily active against gram-positive bacteria including clinically isolated resistant bacteria. it differed from other members of the antibiotic family as it also demonstrated activity against gram-negative bacteria. pholipomycin protected mice from infection with staphylococcus aureus and was nontoxic to mice having an ld50 (i.v.) of 600 mg/kg. besides possessing anti-microbiol activity, pholipomycin, when administered orall ... | 1977 | 413819 |
| 3-epi-deoxynegamycin and leucyl-3-epi-deoxynegamycin produced by streptomyces. | | 1977 | 413822 |
| biosynthesis of thiamin in bacillus subtilis. isolation of mutants accumulating 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate. | thiamin-deficient mutants of bacillus subtilis were characterized by their growth responses to the pyrimidine and thiazole moieties of the vitamin molecule and by cross-feeding tests. all mutants growing on the thiazole moiety and all mutants with an absolute requirement for thiamin fed all those growing on the pyrimidine moiety. no other cross-feeding effects were observed. from the culture fluid of a mutant growing on the thiazole moiety, two compounds were isolated which supported growth of m ... | 1977 | 413878 |
| respiratory manifestations and skin reactivity in the detergent industry. | workers at two continental european enzyme detergent plants were examined to determine respiratory symptomatology, pulmonary function abnormalities and skin reactivity to four enzymes through use of a standardized questionnaire, flow-volume curves and provocative acetylcholine, single breath carbon monoxide, and prick tests. in both plants, the frequency of bronchitis was similar but the frequency of asthmatic or rhinopharyngitic symptoms was different. symptomatology was more frequent in female ... | 1978 | 413892 |
| semisynthetic aminoglycoside antibacterials. 6. synthesis of sisomicin, antibiotic g-52, and novel 6'-substituted analogues of sisomicin from aminoglycoside 66-40c. | the discovery of aminoglycoside 66-40c, a novel dimeric, unsaturated imine produced by micromonospora inyoensis, afforded a versatile intermediate for the synthesis of a variety of sisomicin analogues modified at the 6' position. the conversion of 66-40c into sisomicin, antibiotic g-52, and a series of novel 6'-substituted analogues of sisomicin is described, and the biological activity of the products is discussed. | 1978 | 413921 |
| completion of the replication and division cycle in temperature-sensitive dna initiation mutants of bacillus subtilis 168 at the non-permissive temperature. | | 1977 | 413927 |
| transcriptional specificity of a multisubunit rna polymerase induced by bacillus subtilis bacteriophage pbs2. | bacillus subtilis phage pbs2 induced the synthesis of two temporally defined categories of phage-specified transcripts. the transcription of phage "early" genes was induced almost immediately after infection; this rna synthesis did not require phage protein synthesis. phage "late" gene transcription, on the other hand, was induced at an intermediate time in the lytic cycle; this rna synthesis required the production of phage proteins. both classes of transcription were resistant to the drug rifa ... | 1978 | 413936 |
| macromolecular synthesis in chromosome initiation mutants of bacillus subtilis. | inactivation of the dna b or dna d gene product in bacillus subtilis stimulates rna and protein synthesis. strains containing ts dna b and d mutations have been constructed by introducing the mutations by transformation into a thymine requiring strain which does not lyse during thymine starvation. the consequences of inactivation of these gene products have been assessed by comparing rna and protein synthesis during thymine starvation at the restrictive temperature with the recipient strain. in ... | 1977 | 414065 |
| transformation in bacillus subtilis: biological and physical evidence for a novel dna-intermediate in synchronously transforming cells. | competent b. subtilis cells exposed to transforming dna in the presence of edta bind, but do not take up dna. rapid and almost synchronous uptake of the bound dna is achieved by the addition of mg2+ ions in excess of the edta. at 30 degrees and at 17 degrees comparable numbers of transformants are produced from cells pre-loaded with dna at 30 degrees (after termination of uptake by the addition of dna ase the samples were incubated at 37 degrees). however, almost no transformants are produced wh ... | 1977 | 414066 |
| heterogeneity of the genes coding for 5 s rna in three related strains of the genus bacillus. | | 1977 | 414067 |
| characteristics of a complex formed by a nonintegrated fraction of transforming dna and bacillus subtilis recipient cell constituents. | | 1977 | 414068 |
| on the dose response in b. subtilis transfection: involvement of aggregates in phi 29 transfection. | transfection with phi 29 dna is predominantly caused by multimolecular, protease-sensitive aggregates of dna. a minority of transfecting dna molecules having properties of unit-length phi 29 genome molecules show a quadratic dose response in transfection. | 1977 | 414069 |
| molecular cloning and in vitro transcription of bacillus subtilis plasmid in escherichia coli. | a composite plasmid (pat2010) has been constructed in vitro from rsf2124 and bacillus subtilis ifo3022 plasmid (pat1060) by covalent joining of the two dna molecules by means of escherichia coli dna ligase through the cohesive ends generated by restriction endonuclease ri (ecori) cleavage. the composite plasmid was selected by transformation of e. colic600r-m- with the ligated mixture after enrichment for composite plasmid by preparative agarose gel electrophoresis, and plating of the transforma ... | 1977 | 414072 |
| streptomycin-resistant, asporogenous mutant of bacillus subtilis. | a streptomycin-resistant mutantant of bacillus subtilis that is also asporogenous, was isolated and partially characterized. this strain, srb15, sporulated at a frequency of about 1% compared to the wild type frequency of greater than 70%. the two phenotypes were inseparable by transformation, suggesting that this strain carries a single mutation that causes it to be both streptomycin-resistant and spore-minus. the mutation cotransduces with cysa, the closest auxotrophic marker to the "ribosomal ... | 1977 | 414073 |
| nucleotide sequence of a lysine trna from bacillus subtilis. | a lysine trna (trna1lys) was purified from bacillus subtilis w168 by a consecutive use of several column chromatographic systems. the nucleotide sequence was determined to be pg-a-g-c-c-a-u-u-a-g-c-u-c-a-g-u-d-g-g-d-a-g-a-g-c-a-u-c-u-g-a-c-u-u(u*)-u-u-k-a-psi-c-a-g-a-g-g-m7g(g)-u-c-g-a-a-g-g-t-psi-c-g-a-g-u-c-c-u-u-c-a-u-g-g-c-u-c-a-c-c-aoh, where k and u* are unidentified nucleosides. the nucleosides of u34 and m7g46 were partially substituted with u* and g, respectively. the binding ability of ... | 1977 | 414208 |
| [dna metabolism of a temperature sensitive filamentous mutant of bacillus subtilis sb 19]. | | 1977 | 414466 |
| a counting method for determining the burst size of defective phages from bacillus subtilis. | the defective phages of bacillus subtilis cannot be counted by plating as they do not form plaques. in addition, counting under the electron microscope with latex spheres as an internal standard is not possible. the reliability of a method using escherichia coli phage t4 as a substitute for the latex spheres has been tested and the results compared with those of other methods. using this method, we determined the burst sizes of the defective phages pbs x, pbs y and pbs z under various conditions ... | 1977 | 414658 |
| subunit contacts of the rifamycin binding site of rna polymerase (b. subtilis). | | 1978 | 414752 |
| a template independent, rifampicin sensitive poly (a).poly (u) synthesizing activity present in bacillus subtilis. | | 1978 | 414754 |
| relation between inhibition of bacilli sporulation and synthesis of lytic enzymes. | | 1978 | 414756 |
| purification and characterization of alanine carrier isolated from h-proteins of bacillus subtilis. | alanine transport carrier was isolated and purified from h-proteins of bacillus subtilis. the purified carrier preparation was homogeneous in migration on polyacrylamide gels containing urea or sodium dodecyl sulfate. electrophoresis on polyacrylamide gels containing dodecyl sulfate showed a single band of molecular weight of about 7500. 1 mol alanine was bound/mol carrier protein with a dissociation constant of 0.2 micron. the binding was inhibited by p-chloromercuribenzoate and the inhibition ... | 1978 | 414914 |
| [bacillus subtilis mutant with altered ability for heterologous transformation]. | mutant strain of bacillus subtilis, which produced in certain conditions significantly reduced quantity of trnasformants during transformation by homologous dna, as compared with transformation by heterologous dna from bac. aterrimus, is isolated. the ability to transfection by phage spo1 dna and the efficiency of infection of the mutant by this phage are also decreased. the causes of such alterated properties are discussed. | 1978 | 414961 |
| differential antimicrobial activities of anthracycline antibiotics on rec-bacillus subtilis. | | 1978 | 415030 |
| spore location patterns in sporulating doublets of bacillus cereus and bacillus megaterium, derived from single doublet isolates with differing sporulation geometry. | the distribution of spore loci in pairs of bacillus cereus and b. megaterium showed different degrees of polarization from random location towards location at the old end of the sporangium. when individual doublets containing both spores at either the old or new ends of the sporangia were isolated by micromanipulation, subsequent culture showed the same spore location patterns. | 1978 | 415036 |
| mapping of a genetic locus that affects glycerol 3-phosphate transport in bacillus subtilis. | two types of fosfomycin-resistant mutants of bacillus subtilis were isolated. mutants of the first type (glpt mutants) were resistant to at least 200 microgram of fosfomycin per ml and failed to take up exogenous glycerol 3-phosphate. mutants of the second type were resistant to lower concentrations of fosfomycin and transported glycerol-3-phosphate as efficiently as wild-type bacteria. the glpt mutations, but not the mutations in the second type of fosfomycin-resistant mutants, map in the cysa- ... | 1978 | 415047 |
| functional expression of two bacillus subtilis chromosomal genes in escherichia coli. | ecori-cleaved deoxyribonucleic acid segments carrying two genes from bacillus subtilis, pyr and leu, have been cloned in escherichia coli by insertion into a derivative of the e. coli bacteriophage lambda. lysogenization of pyrimidine- and leucine-requiring auxotrophs of e. coli by the hybrid phages exhibited prototrophic phenotypes, suggesting the expression of b. subtilis genes in e. coli. upon induction, these lysogens produced lysates capable of transducing e. coli pyr and leu auxotrophs to ... | 1978 | 415049 |
| bacteriocin and antibiotic resistance plasmids in bacillus cereus and bacillus subtilis. | a number of plasmids have been isolated as covalently closed circular dnas from strains of bacillus cereus and b. subtilis. from 12 out of 15 strains of b. cereus, plasmids could be isolated. most of the b. cereus strains contained two or more plasmids. their molecular weights ranged from 1.6 x 10(6) to 105 x 10(6). bacteriocin production could be attributed to a 45 x 10(6)-dalton plasmid (pbc7) from b. cereus dsm 336, and tetracycline resistance to a 2.8 x 10(6) plasmid (pbc16) from b. cereus g ... | 1978 | 415051 |
| study of pole assembly in bacillus subtilis by computer reconstruction of septal growth zones seen in central, longitudinal thin sections of cells. | the septal growth of bacillus subtilis 168/s has been studied by making a number of observations from thin sections of cells from exponentially growing cultures. the process was initiated by the formation of a new cross wall under a preexisting layer of cylindrical wall. an annular notch appeared to cut through the overlying wall and presumably allowed the cross wall to split into two layers of peripheral wall. during this initial notching process, two raised bands of wall material were produced ... | 1978 | 415053 |
| catabolic conversion of sepiapterin to 6-(1-carboxyethoxy)pterin by bacillus subtilis. | structural elucidation of an intensely blue fluorescent compound (a) formed from sepiapterin by bacillus subtilis is described. the structure of the catabolite (a) was found to be 2-amino-6-(1-carboxyethoxy)-4(3h)-pteridinone (9) from both spectroscopic and degradation studies. this was confirmed by an unambiguous synthesis of 9. the stereochemical structure of the side chain at the 6-position of a was confirmed to be the l(or s) configuration, as in sepiapterin, by analysis of the lactic acid f ... | 1978 | 415056 |
| trail phenomena in connexion with serratia phages and marcescin-like activities. | | 1978 | 415120 |
| effects of the transciption inhibitory protein, tf1, on phage sp01 promoter complex formation and stability. | | 1977 | 415142 |
| translation of rnas synthesized in vivo and in vitro from bacteriophage sp82 dna. | the synthesis of 69 phage-specific polypeptides during the infection of bacillus subtilis with bacteriophage sp82 was detected by pulse-labeling, one-dimensional electrophoresis, and autoradiography. sp82 virions were found to contain approximately 22 polypeptides, most of which were synthesized late in infection; evidence was obtained for the processing of the major virion protein. rnas extracted at different times during infection were translated by using an escherichia coli cell-free extract. ... | 1978 | 415151 |
| incorporation of label from ribose into 5-(4',5'-dihydroxypentyl) uracil of bacteriophage sp15 dna. | radioactively labeled ribose was incorporated into the glucosylated deoxynucleoside monophosphate of 5-(4',5'-dihydroxypentyl)uracil of bacteriophage sp15 dna to a greater extent than into the other pyrimidine deoxynucleoside monophosphates. results from formic acid hydrolysis of the deoxynucleoside monophosphates to their bases suggest that label from ribose is incorporated into the dihydroxypentyl side chain of 5-(4',5'-dihydroxypentyl)uracil. | 1978 | 415152 |
| isolation of a bacillus subtilis 168 derivative sensitive to defective bacteriophage pbsx. | a derivative of bacillus subtilis strain 168 sensitive to defective bacteriophages pbsx and pbsz has been isolated. this particular strain, rub824, carries the genetic information for defective bacteriophage pbsx. | 1978 | 415153 |
| [germination and outgrowth of spores and proliferation of outgrown vegetative cells in the peritoneal cavity of mice intraperitoneally injected with bacillus cereus or bacillus subtilis spores (author's transl)]. | | 1977 | 415160 |
| [processes regulating division, sporulation and synthesis of metabolites by microorganisms]. | | 1977 | 415214 |
| bacteriophage infection of minicells: a general method for identification of "in vivo" bacteriophage directed polypeptide biosynthesis. | | 1977 | 415222 |
| cloning of bacillus subtilis leucina a, b and c genes with escherichia coli plasmids and expression of the leuc gene in e. coli. | the leucine genes of bacillus subtilis have been cloned directly from the chromosomal dna into escherichia coli leub cells by selection for the leu+ phenotype using rsf2124 as a vector plasmid. the hybrid plasmid designated rsf2124-b.leu contained a 4.2 megadalton fragment derived from b. subtilis dna, including the leu genes. the fragment had one site susceptible to ecori* and another site susceptible to bamni endonuclease. among the three fragments produced by ecori* and bamni endonucleases, t ... | 1978 | 415224 |
| synthesis of phage 2c-dna in permeabilized b. subtilis. | | 1978 | 415225 |
| mutation induction with uv- and x-radiations in spores and vegetative cells of bacillus subtilis. | spores and vegetative cells of bacillus subtilis strains with various defects in dna-repair capacities (hcr-, ssp-, hcr-ssp-) were irradiated with uv radiation or x-rays. induced mutation frequency was determined from the observed frequency of prototrophic reversion of a suppressible auxotrophic mutation. at equal physical dose, after either uv- or x-irradiation, spores were more resistant to mutations as well as to killing than were vegetative cells. however, quantitative comparison revealed th ... | 1978 | 415232 |
| photodynamic action of fluorescein dyes in dna-damage and in vitro inactivation of transforming dna in bacteria. | | 1978 | 415233 |
| effects of cyanine dye membrane probes on cellular properties. | | 1978 | 415251 |
| new cluster of ribosomal genes in bacillus subtilis with regulatory role in sporulation. | | 1978 | 415254 |
| utilization of a depsipeptide substrate for trapping acyl-enzyme intermediates of penicillin-sensitive d-alanine carboxypeptidases. | the penicillin-sensitive d-alanine carboxypeptidases of bacillus subtilis, escherichia coli, and staphylococcus aureus catalyzed the hydrolysis of the d-lactic acid residue from the depsipeptide diacetyl-l-lysyl-d-alanyl-d-lactic acid. the ester substrate was hydrolyzed faster than the peptide analogue, diacetyl-l-lysyl-d-alanyl-d-alanine, by the b. subtilis (15-fold) and e. coli (4-fold) carboxypeptidases, presumably because acylation (k(2)), which is the rate-limiting step of the peptidase rea ... | 1978 | 415311 |
| [paper chromography of semisynthetic penicillins]. | | 1977 | 415315 |
| metals as mutagens. | a number of metals are mutagenic in bacteria or phage. these include compounds of arsenic, chromium, copper, iron, manganese, molybdenum, platinum, and selenium. compounds containing alumium, antimony, arsenic, cadmium, copper, lead, mercury, nickel, and tellium have been shown to induce chromosomal aberrations or abnormal cell divisions in animal or plant cells. genetic evidence suggests that arsenic, chromium, and molybdenum compounds may influence the accuracy of dna repair processes in micro ... | 1977 | 415523 |
| [testing the sterility of injection preparations of penicillin series antibiotics]. | the efficiency of 3 variants of the method for determination of microbial flora was compared on the injection preparation of potassium benzylpenicillin artificially infected with staph. aureus 209p and the spores of bac. subtilis atcc 6633 in different doses and with different amounts of the preparation in the vials. the procedure of the preparation dissolution in the vial with the thioglycol medium containing penicillinase proved to be most effective. the microbe detection amounted to 100 per c ... | 1978 | 415660 |
| sensitive bioassay for vancomycin. | an accurate and sensitive assay for vancomycin in serum and body fluids has not been available. this paper reports an assay for vancomycin that can detect serum and fluid levels as low as 0.8 mug/ml. a disk diffusion technique was designed employing buffered glucose minimal salts agar and bacillus subtilis as an indicator strain. a linear relationship was obtained between zone diameter and concentration for vancomycin standards from 0.8 to 50 mug/ml prepared in pooled human serum. results were a ... | 1978 | 415661 |
| [the significance of in-vitro tests with respect to testing of substances for carcinogenic activities (author's transl)]. | the actual possibilities to test chemical substances for carcinogenic activities are critically assessed. short-term-tests are especially discussed. by these tests--now utilized for screening of chemicals--specific biological activities can be determined, which correlate with carcinogenic effects in vivo. the phase of checking and improving the vitro-tests is not yet finished with regard to the rate of correlation of the results from those tests and the results from long-term experiments in anim ... | 1977 | 415699 |
| effects of staphylococcin 1580 on cells and membrane vesicles of bacillus subtilis w23. | 1. uptake of l-glutamic acid is inhibited, and preaccumulated l-glutamic acid is released from bacillus subtilis cells treated with staphylococcin 1580. uptake of alpha-methylglycoside is enhanced at low bacteriocin concentrations and inhibited by excess bacteriocin. 2. inhibition of amino acid uptake into membrane vesicles is somewhat less sensitive to staphylococcin 1580 than uptake into whole cells under similar conditions, when the bacteriocin concentration is expressed per weight unit of me ... | 1978 | 415763 |
| [expression of bacillus subtilis riboflavin operon in escherichia coli cells in the form of hybrid plasmids ppr1 and ppr2]. | | 1978 | 415855 |
| role of the 21,000 molecular weight polypeptide of bacillus subtilis rna polymerase in rna synthesis. | dna-dependent rna polymerase from bacillus subtilis contains a 21,000 molecular weight (21k) peptide subunit. this subunit was purified and added to 21k-depleted polymerase isolated from both uninfected and sp85-infected b. subtilis. the effect of the subunit on total rna synthesis, on enzyme-dna binding, on rna chain initiation and elongation, and on enzymatic specificity were examined. a comparison was made of the effects of the 21k peptide and nacl on polymerase activity, rna chain elongation ... | 1978 | 416017 |
| isolation of 4-(1'-d-ribitylamino)-5-amino-2,6-dihydroxypyrimidine from a riboflavin-adenine-deficient mutant of bacillus subtilis. | studies were carried out to determine possible intermediates involved in the biosynthetic pathway of riboflavin, using resting cells of a riboflavin-adenine-deficient mutant, bacillus subtilis aj1988. the cells excreted 6,7-dimethyl-8-ribityllumazine, the end product in the biosynthetic pathway, into the incubation medium in large amounts. the addition of glyoxal caused a large accumulation of a green fluorescent compound; an inverse relation was observed between the formation of the lumazine an ... | 1978 | 416026 |