| larval control of anopheles (nyssorhinchus) darlingi using granular formulation of bacillus sphaericus in abandoned gold-miners excavation pools in the brazilian amazon rainforest. | use of a bacillus sphaericus based mosquito larvicide was evaluated as an intervention for malaria vector control at a mining site in amapá, brazil. impacts on larval and adult densities of the primary vector anopheles darlingi were measured over the course of a 52 week study period. | 2013 | 23740074 |
| biological treatment and modeling aspect of btex abatement process in a biofilter. | in the present work, a laboratory scale corn-cob based biofilter inoculated with bacillus sphaericus (mtcc 8103) was used for degradation of btex for a period of 86 days. the overall performance of a biofilter evaluated in terms of its elimination capacity by using 3-d mesh technique. maximum removal efficiency was found more than 96.43% for all four compounds in each phase of experiments. a maximum elimination capacity (ec) of 60.89 gm(-3)h(-1) of the biofilter was obtained at inlet btex load o ... | 2013 | 23732917 |
| potency and persistence of the bacterial mosquitocide bacillus sphaericus against culicine mosquitoes under field conditions. | the biopesticide bacillus sphaericus represents one of the important tools used in the control of mosquito larvae after chemical insecticides. the present study was conducted to investigate the efficacy b. sphaericus 2362 (vectolex) in two different technical powders, abg-6232 and abg-6491 against larvae of two mosquitoes, culex pipiens and aedescaspius in el-arish city, north sinai, egypt. the mosquito larvae were collected from polluted and fresh water (cx. pipiens) and saline water (ae. caspi ... | 2013 | 23697020 |
| molecular characterization of mosquitocidal bacillus sphaericus isolated from tamil nadu, india. | forty-two bacillus sphaericus strains were isolated from soil around tamil nadu, india. the phylogenetic relationship among the b. sphaericus isolates was analysed by rep-pcr and multiplex pcr was performed for the detection of mosquito larvicidal genes bina, binb, mtx1, mtx2 and mtx3 in b. sphaericus isolates. according to the rep-pcr band pattern, b. sphaericus isolates were divided into group a comprising i-xi clusters and group b comprising cluster xii. three of the isolates bstn01, 23 and 2 ... | 2013 | 23648218 |
| identification of multiple putative s-layer genes partly expressed by lysinibacillus sphaericus jg-b53. | lysinibacillus sphaericus jg-b53 was isolated from the uranium mining waste pile haberland near johanngeorgenstadt, germany. previous studies have shown that many bacteria that have been isolated from these heavy metal contaminated environments possess surface layer (s-layer) proteins that enable the bacteria to survive by binding metals with high affinity. conversely, essential trace elements are able to cross the filter layer and reach the interior of the cell. this is especially true of the s ... | 2013 | 23579690 |
| novel organic solvent-tolerant esterase isolated by metagenomics: insights into the lipase/esterase classification. | in order to isolate novel organic solvent-tolerant (ost) lipases, a metagenomic library was built using dna derived from a temperate forest soil sample. a two-step activity-based screening allowed the isolation of a lipolytic clone active in the presence of organic solvents. sequencing of the plasmid prbest recovered from the positive clone revealed the presence of a putative lipase/esterase encoding gene. the deduced amino acid sequence (rbest1) contains the conserved lipolytic enzyme signature ... | 2013 | 23560782 |
| assessment of functional and genetic diversity of aerobic endospore forming bacilli from rhizospheric soil of phyllanthus amarus l. | fifty two aerobic and endospore forming bacilli (aefb) strains were recovered from rhizospheric soil of phyllanthus amarus. morphological, biochemical and molecular characterization by 16s rdna gene sequencing has shown that these bacterial strains belong to six different genera of aefb i.e. bacillus, brevibacillus, lysinibacillus, paenibacillus, terribacillus and jeotgalibacillus. analysis of their pgp activities has shown that 92.30 % strains produced indole acetic acid hormone, 86.53 % of the ... | 2013 | 23526192 |
| metal tolerance and larvicidal activity of lysinibacillus sphaericus. | lysinibacillus sphaericus is a spore-forming bacterium used in the biological control of mosquitoes and in bioremediation. mosquito larvae exposed to heavy metals are tolerant to concentrations above the permissible limit for industrial residual waters. in this work, we characterize 51 l. sphaericus strains for metal tolerance and larvicidal activity against culex quinquefasciatus. lysinibacillus sphaericus ot4b.2, ot4b.20, ot4b.25, ot4b.26 and ot4b.58 were as toxic as the spores of the referenc ... | 2013 | 23504213 |
| nanobiotechnology advanced antifouling surfaces for the continuous electrochemical monitoring of glucose in whole blood using a lab-on-a-chip. | in the current work we have developed a lab-on-a-chip containing embedded amperometric sensors in four microreactors that can be addressed individually and that are coated with crystalline surface protein monolayers to provide a continuous, stable, reliable and accurate detection of blood glucose. it is envisioned that the microfluidic device will be used in a feedback loop mechanism to assess natural variations in blood glucose levels during hemodialysis to allow the individual adjustment of gl ... | 2013 | 23478879 |
| lysinibacillus contaminans sp. nov., isolated from surface water. | a gram-positive-staining, aerobic, endospore-forming bacterium, isolated as a contamination from an enrichment of enteric bacteria from surface water, was studied using a polyphasic taxonomic approach. 16s rrna gene sequence similarity comparisons revealed that strain fst3a(t) was grouped in the genus lysinibacillus, most closely related to lysinibacillus xylanilyticus xdb9(t) (98.1%), lysinibacillus parviboronicapiens bam-582(t) and lysinibacillus sphaericus dsm 28(t) (both 98.0%). the 16s rrna ... | 2013 | 23435244 |
| mosquito larvicidal and pupicidal activity of euphorbia hirta linn. (family: euphorbiaceae) and bacillus sphaericus against anopheles stephensi liston. (diptera: culicidae). | to explore the larvicidal and pupicidal activity of euphorbia hirta (e. hirta) leaf extract and bacillus sphaericus (b. sphaericus) against the malarial vector, anopheles stephensi (an. stephensi). | 2013 | 23339910 |
| engineering of corynebacterium glutamicum for high-yield l-valine production under oxygen deprivation conditions. | we previously demonstrated efficient l-valine production by metabolically engineered corynebacterium glutamicum under oxygen deprivation. to achieve the high productivity, a nadh/nadph cofactor imbalance during the synthesis of l-valine was overcome by engineering nad-preferring mutant acetohydroxy acid isomeroreductase (ahair) and using nad-specific leucine dehydrogenase from lysinibacillus sphaericus. lactate as a by-product was largely eliminated by disrupting the lactate dehydrogenase gene l ... | 2013 | 23241971 |
| ccpa-mediated enhancement of sugar and amino acid metabolism in lysinibacillus sphaericus by nmr-based metabolomics. | lysinibacillus sphaericus is a bacterium incapable of metabolizing sugars with the sole exception of n-acetylglucosamine. to unravel the regulatory role of catabolite control protein a (ccpa) in the sugar metabolism of l. sphaericus, a ccpa deficient mutant was constructed by homologous recombination. the mutant showed growth deficiency and a low efficiency of carbon and energy utilization. nmr spectroscopy in combination with multivariate data analysis revealed that the metabolome of l. sphaeri ... | 2012 | 22856458 |
| inoculation of bacillus sphaericus upmb-10 to young oil palm and measurement of its uptake of fixed nitrogen using the ¹⁵n isotope dilution technique. | there are increasing applications of diazotrophic rhizobacteria in the sustainable agriculture system. a field experiment on young immature oil palm was conducted to quantify the uptake of n derived from n₂ fixation by the diazotroph bacillus sphaericus strain upmb-10, using the ¹⁵n isotope dilution method. eight months after ¹⁵n application, young immature oil palms that received 67% of standard n fertilizer application together with b. sphaericus inoculation had significantly lower ¹⁵n enrichm ... | 2012 | 22446306 |
| influence of temperature on the effectiveness of a biogenic carbonate surface treatment for limestone conservation. | so far, most studies on microbiologically induced carbonate precipitation for limestone conservation have been performed at temperatures optimal for the activity of the calcinogenic bacteria (i.e., 20-28 °c). successful application in practice, however, requires adequate performance in a wide range of environmental conditions. therefore, the aim of this study was to select microorganisms that are most suited for biodeposition at temperatures relevant for practice. in a first step, ureolytic micr ... | 2013 | 22426701 |
| generation of mariner-based transposon insertion mutant library of bacillus sphaericus 2297 and investigation of genes involved in sporulation and mosquito-larvicidal crystal protein synthesis. | bacillus sphaericus has been used with great success in mosquito control programs worldwide. under conditions of nutrient limitation, it undergoes sporulation via a series of well defined morphological stages. however, only a small number of genes involved in sporulation have been identified. to identify genes associated with sporulation, and to understand the relationship between sporulation and crystal protein synthesis, a random mariner-based transposon insertion mutant library of b. sphaeric ... | 2012 | 22404546 |
| expression and purification of the active soluble form of bacillus sphaericus binary toxin for structural analysis. | the binary toxin produced from bacillus sphaericus is highly toxic against larvae of culex and anopheles mosquitoes. the two major components of the binary toxin are 42-kda bina and 51-kda binb, which are produced as crystalline inclusions during sporulation. currently, there is no detailed knowledge of the molecular mechanism of the binary toxin, mainly due to the lack of structural information. herein, we describe an expression protocol with modified conditions allowing production of soluble, ... | 2012 | 22381463 |
| biosensing for the environment and defence: aqueous uranyl detection using bacterial surface layer proteins. | the fabrication of novel uranyl (uo(2)(2+)) binding protein based sensors is reported. the new biosensor responds to picomolar levels of aqueous uranyl ions within minutes using lysinibacillus sphaericus jg-a12 s-layer protein tethered to gold electrodes. in comparison to traditional self assembled monolayer based biosensors the porous bioconjugated layer gave greater stability, longer electrode life span and a denser protein layer. biosensors responded specifically to uo(2)(2+) ions and showed ... | 2010 | 22399904 |
| optimization of mosquitocidal toxin synthesis from bacillus sphaericus using gene fusions. | β-galactosidase gene fusions have been used to monitor the progress of mosquito-larvicidal-toxin gene expression in bacillus sphaericus strain 2362. β-galactosidase estimation in cells from late-growth-phase batch cultures was compared with larvicidal toxicity after incubation for 48 h. conditions which promoted efficient sporulation, such as plentiful trace elements and relatively crude protein sources (soybean or cottonseed flours), enhanced reporter gene expression and provided high toxicity. ... | 1996 | 24415078 |
| preparation of a flowable liquid bacterial insecticide based on bacillus sphaericus. | fermenter-produced bacillus sphaericus 2362 was formulated into a thick, dark flowable liquid concentrate containing 4.8×10(9) c.f.u./ml and charcoal as protector against ultraviolet light. the potencies of the formulation against l4 culex pipiens quinquefasciatus before and after storage for 2 years were 5714 and 5862 international toxic units (itu), respectively, when compared with a standardized b. sphaericus from the who at 1000 itu. in field trials, treatment at 1.01/ha gave 96 to 100% cont ... | 1991 | 24425273 |
| delignification and enhanced gas release from soil containing lignocellulose by treatment with bacterial lignin degraders. | the aim of the study was to isolate bacterial lignin-degrading bacteria from municipal solid waste soil, and to investigate whether they could be used to delignify lignocellulose-containing soil, and enhance methane release. | 2017 | 28393443 |
| recombinant expression, characterization and application of a dihydrolipoamide dehydrogenase with diaphorase activity from bacillus sphaericus. | diaphorases are flavin-containing enzymes with potential applications in biotransfomation reactions, biosensor design and in vitro diagnostic tests. in this communication, we describe recombinant expression, characterization and application of a lipoamide dehydrogenase (dld) with diaphorase activity from a strain of bacillus sphaericus. the dld gene consisting of 1413 bp encoding a protein of 470 amino acids was expressed in escherichia coli bl21 (de3) and the recombinant enzyme was characterize ... | 2017 | 28597164 |
| medium optimization for improved production of dihydrolipohyl dehydrogenase from bacillus sphaericus pad-91 in escherichia coli. | dihydrolipohyl dehydrogenase (dld) is a fad-dependent enzyme that catalyzes the reversible oxidation of dihydrolipoamide. herein, we report medium optimization for the production of a recombinant dld with nadh-dependent diaphorase activity from a strain of bacillus sphaericus pad-91. the dld gene that consisted of 1413 bp was expressed in escherichia coli bl21 (de3), and its enzymatic properties were studied. the composition of production medium was optimized using one-variable-at-a-time method ... | 2017 | 28573449 |
| the lspc3-41i restriction-modification system is the major determinant for genetic manipulations of lysinibacillus sphaericus c3-41. | lysinibacillus sphaericus has been widely used in integrated mosquito control program and it is one of the minority bacterial species unable to metabolize carbohydrates. in consideration of the high genetic conservation at genomic level and difficulty of genetic horizontal transfer, it is hypothesized that effective restriction-modification (r-m) systems existed in mosquitocidal l. sphaericus. | 2017 | 28525986 |
| identification of cry48aa/cry49aa toxin ligands in the midgut of culex quinquefasciatus larvae. | a binary mosquitocidal toxin composed of a three-domain cry-like toxin (cry48aa) and a binary-like toxin (cry49aa) was identified in lysinibacillus sphaericus. cry48aa/cry49aa has action on culex quinquefasciatus larvae, in particular, to those that are resistant to the bin binary toxin, which is the major insecticidal factor from l. sphaericus-based biolarvicides, indicating that cry48aa/cry49aa interacts with distinct target sites in the midgut and can overcome bin toxin resistance. this study ... | 2017 | 28780070 |
| cation-chelation and ph induced controlled switching of the non-fouling properties of bacterial crystalline films. | we report the controlled loss of the anti-fouling activity of the s-layer protein sbpa from lysinibacillus sphaericus (ccm2177). this protein forms crystal-like films with square lattice (p4) via self-assembly on almost any type of surfaces. such engineered bioinspired nanometric membranes are known by their excellent preventive performance under biological conditions. however, their exposure to certain treatments can lead to gradual degradation of the s-protein layer. in this work, two distinct ... | 2017 | 28704713 |
| a genome-scale metabolic reconstruction of lysinibacillus sphaericus unveils unexploited biotechnological potentials. | the toxic lineage (tl) of lysinibacillus sphaericus has been extensively studied because of its potential biotechnological applications in biocontrol of mosquitoes and bioremediation of toxic metals. we previously proposed that l. sphaericus tl should be considered as a novel species based on a comparative genomic analysis. in the current work, we constructed the first manually curated metabolic reconstruction for this species on the basis of the available genomes. we elucidated the central meta ... | 2017 | 28604819 |
| efficacy of the vegetative cells of lysinibacillus sphaericus for biological control of insecticide-resistant aedes aegypti. | the control of aedes aegypti is usually based on chemical insecticides, but the overuse of these compounds has led to increased resistance. the binary toxin produced by lysinibacillus sphaericus in the final stages of sporulation is used for mosquito control due to its specificity against the culicid larvae; however, it has been proved that ae. aegypti is refractory for this toxin. currently, there is no evidence of the use of l. sphaericus vegetative cells for mosquito biocontrol. therefore, in ... | 2017 | 28490350 |
| biocementation of concrete pavements using microbially induced calcite precipitation. | in this study, the feasibility of introducing calcite-forming bacteria into concrete pavements to improve their mechanical performance was investigated. lysinibacillus sphaericus wj-8, which was isolated in a previous study and is capable of exhibiting high urease activity and calcite production, was used. when analyzed via scanning electron microscopy (sem) and x-ray diffraction, wj-8 showed a significant amount of calcite precipitation. the compressive strength of cement mortar mixed with wj-8 ... | 2017 | 28478659 |
| differential expression of duplicated binary toxin genes bina/binb in lysinibacillus sphaericus c3-41. | due to the self-produced binary toxins bina/binb, some lysinibacillus sphaericus isolates exhibit high toxicity against mosquito larvae and are utilized in mosquito control programmes. previous whole-genome sequencing revealed the presence of a large plasmid pbsph in l. sphaericus c3-41, which contains a 30·5-kb duplication of the genome including the binary toxin genes bina/binb. this was confirmed by southern blot and qpcr experiments in this study. mutants of l. sphaericus c3-41, bearing disr ... | 2017 | 28474344 |
| molecular analysis of culex quinquefasciatus larvae responses to lysinibacillus sphaericus bin toxin. | lysinibacillus sphaericus produces the mosquito larvicidal binary toxin consisting of bina and binb, which are both required for toxicity against culex and anopheles larvae. the molecular mechanisms behind bin toxin-induced damage remain unexplored. we used whole-genome microarray-based transcriptome analysis to better understand how culex larvae respond to bin toxin treatment at the molecular level. our analyses of culex quinquefasciatus larvae transcriptome changes at 6, 12, and 18 h after bin ... | 2017 | 28406958 |