| composition of clusters and building blocks in amylopectins from maize mutants deficient in starch synthase iii. | branches in amylopectin are distributed along the backbone. units of the branches are building blocks (smaller) and clusters (larger) based on the distance between branches. in this study, composition of clusters and building blocks of amylopectins from dull1 maize mutants deficient in starch synthase iii (ssiii) with a common genetic background (w64a) were characterized and compared with the wild type. clusters were produced from amylopectins by partial hydrolysis using α-amylase of bacillus am ... | 2013 | 24229421 |
| identification and characterization of novel surfactins produced by fungal antagonist bacillus amyloliquefaciens 6b. | the broad-spectrum fungal antagonist, bacillus amyloliquefaciens 6b (ba6b), isolated from the jakhao coast of kutch, india, was investigated for its antifungal metabolites using mass spectrometry. the cyclic lipopeptides harvested from the cell-free fermentation broth of ba6b by acid precipitation and subsequently dissolved in methanol were subjected to liquid chromatography coupled with electrospray ionization mass spectrometry (lc-esi-ms/ms) for their identification and sequence determination. ... | 2014 | 24164289 |
| dual mode of action of amylolysin: a type-b lantibiotic produced by bacillus amyloliquefaciens ga1. | the partial genome sequencing of bacillus amyloliquefaciens ga1 led to the identification of the aml gene cluster involved in the synthesis of the novel lantibiotic named amylolysin. pure amylolysin was shown to have an antibacterial activity toward gram-positive bacteria including methicillin resistant staphylococcus aureus. the lantibiotic was also found efficient to inhibit the growth of listeria monocytogenes strains on poultry meat upon a long storage at 4°c. in silico analyses of the aml g ... | 2014 | 24164268 |
| antibacterial activity of the lipopetides produced by bacillus amyloliquefaciens m1 against multidrug-resistant vibrio spp. isolated from diseased marine animals. | in this work, the antibacterial activity of the lipopeptides produced by bacillus amyloliquefaciens m1 was examined against multidrug-resistant vibrio spp. and shewanella aquimarina isolated from diseased marine animals. a new and cheap medium which contained 1.0 % soybean powder, 1.5 % wheat flour, ph 7.0 was developed. a crude surfactant concentration of 0.28 mg/ml was obtained after 18 h of 10-l fermentation and diameter of the clear zone on the plate seeded with vibrio anguillarum was 34 mm. ... | 2014 | 24132666 |
| investigation on mechanism of cr(vi) reduction and removal by bacillus amyloliquefaciens, a novel chromate tolerant bacterium isolated from chromite mine soil. | a strain csb 9 isolated from chromite mine soil of sukinda, india was identified as bacillus amyloliquefaciens based on biochemical and 16s rrna gene sequencing. the strain exhibited relatively high tolerance to cr(vi) (⩽900mgl(-1)) and fast reduction rate of 2.22mg cr(vi) l(-1)h(-1), under optimized conditions of 100mgl(-1) cr(vi), ph 7 and temperature 35°c within 45h. mechanism of cr(vi) reduction as well as nature and fate of the reduced product were studied to determine the scope of removal ... | 2014 | 24091247 |
| use of silkworms to evaluate the pathogenicity of bacteria attached to cedar pollen. | injection of a japanese cedar pollen suspension into silkworm hemolymph kills the silkworms. a certain species of bacteria proliferated in the hemolymph of the dead silkworms. a 16s rdna analysis demonstrated that the proliferating bacteria were bacillus cereus, bacillus thuringiensis, bacillus weihenstephanensis, and bacillus amyloliquefaciens. among them, b. cereus, b. thuringiensis, and b. weihenstephanensis exhibited hemolysis against sheep red blood cells and were lethal to mice. a culture ... | 2013 | 24071577 |
| sequence characterization and computational analysis of the non-ribosomal peptide synthetases controlling biosynthesis of lipopeptides, fengycins and bacillomycin d, from bacillus amyloliquefaciens q-426. | lipopeptides secreted by bacteria attract interest because of their uses in biomedicine, biotechnology and food technology; however, harnessing their megasynthases (non-ribosomal peptide synthetases, nrpss) has met with some difficulties in heterologous expression and crystallization. here, we used similarity and phylogenetic analysis of nrps sequences, including the fengycin and iturin family synthetases from bacillus spp., and have developed a novel approach for delineating the length and boun ... | 2013 | 24068498 |
| nmr spectroscopic and ms/ms spectrometric characterization of a new lipopeptide antibiotic bacillopeptin b1 produced by a marine sediment-derived bacillus amyloliquefaciens sh-b74. | | 2014 | 24064466 |
| purification and partial characterization of bacillomycin l produced by bacillus amyloliquefaciens k103 from lemon. | bacillus amyloliquefaciens k103 isolated from a lemon sample was used as a biocontrol agent to suppress rhizoctonia solani kühn and other fungal plant pathogens. two antifungal compounds were purified from the culture broth using acid precipitation, gel permeation chromatography, and reversed-phase high-performance liquid chromatography. matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis indicated that the antifungal compounds were two isomers similar to bacill ... | 2013 | 24043450 |
| bacillus amyloliquefaciens laccase--from soil bacteria to recombinant enzyme for wastewater decolorization. | one hundred wild type strains of bacillus sp. were isolated from industrial and agricultural soil across serbia and screened for laccase activity. three strains showed high laccase activity temperature optimum of 65 and 80 °c towards abts. a new laccase gene from the strain with highest temperature optimum, namely bacillus amyloliquefaciens 12b was cloned and expressed in escherichia coli. recombinant laccase degraded dye reactive blue 52 at ph 7.0 and ph 4.0 and at elevated temperature, while f ... | 2013 | 23994699 |
| [teichoic acids of three type strains of the "bacillus subtilis" group, bacillus mojavensis vkm b-2650, bacillus amyloliquefaciens subsp. amyloliquefaciens vkm b-2582, and bacillus sonorensis vkm b-2652]. | cell walls of three type strains of the "bacillus subtilis" group, bacillus mojavensis vkm b-2650, bacillus amyloliquefaciens subsp. amyloliquefaciens vkm b-2582, and bacillus sonorensis vkm b-2652, are characterized by the individual set of teichoic acids. all strains contained 1,3-poly(glycerophosphates), unsubstituted, acylated with d-alanine, and glycosylated. the latter differ in the nature of the monosaccharide residue. teichoic acids of b. mojavensis vkm b-2650(t) and b. amyloliquefaciens ... | 2014 | 25509395 |
| [immunocytochemical visualization of p185(her2) receptor by antibody fused with dibarnase and conjugate of barstar with colloidal gold]. | we studied the localization of transmembrane receptor p185(her2) in skov-3 and bt-474 cancer cells by fluorescence, confocal and electron immunomicroscopy. p185(her2) is a marker of breast and ovarian tumors, it is considered as a target for anticancer therapy. it is extremely important to choose a universal immunicytotoxic agent applicable, first, to study the distribution of p185(her2) in cancer cells, secondly, to remove p185(her2) from the cell surface and, thirdly, to eliminate target cells ... | 2014 | 25509353 |
| protective effect of bacillus amyloliquefaciens against infections of citrus aurantium seedlings by phoma tracheiphila. | isolate teb1 an antagonistic endophytic bacterium, obtained from citrus leaves and identified as bacillus amyloliquefaciens by 16s rdna sequencing, was used for the biological control of mal secco disease of citrus aurantium seedlings caused by the mitosporic fungus phoma tracheiphila. the isolate teb1 exhibited a good in vitro activity against p. tracheiphila in dual cultures as well as with the well diffusion method. c. aurantium seedlings watered with a suspension of teb1 cells showed a reduc ... | 2014 | 23990072 |
| characterization of aerobic spore-forming bacteria associated with industrial dairy processing environments and product spoilage. | due to changes in the design of industrial food processing and increasing international trade, highly thermoresistant spore-forming bacteria are an emerging problem in food production. minimally processed foods and products with extended shelf life, such as milk products, are at special risk for contamination and subsequent product damages, but information about origin and food quality related properties of highly heat-resistant spore-formers is still limited. therefore, the aim of this study wa ... | 2013 | 23973839 |
| effects of dietary supplementation of bacillus amyloliquefaciens cect 5940 and enterococcus faecium cect 4515 in adult healthy dogs. | the aim of this study was to evaluate the effect of a probiotic preparation consisting of two probiotic strains, bacillus amyloliquefaciens cect 5940 and enterococcus faecium cect 4515 (each 5 · 10(8) cfu/g feed), on faecal consistency, faecal microbiology and nutrient digestibility in adult healthy dogs. sixteen beagles (eight males and eight females) were divided into two groups: the control group (con), which was fed the basal diet, and the probiotic group (pro), which received the basal diet ... | 2013 | 23952642 |
| production and characterization of keratinolytic protease from new wool-degrading bacillus species isolated from egyptian ecosystem. | novel keratin-degrading bacteria were isolated from sand soil samples collected from minia governorate, egypt. in this study, the isolates were identified as bacillus amyloliquefaciens ma20 and bacillus subtilis ma21 based on morphological and biochemical characteristics as well as 16s rrna gene sequencing. b. amyloliquefaciens ma20 and b. subtilis ma21 produced alkaline keratinolytic serine protease when cultivated in mineral medium containing 1% of wool straight off sheep as sole carbon and ni ... | 2013 | 23936776 |
| characterization of internal structure of maize starch without amylose and amylopectin separation. | normal maize starch was used to characterize the internal structure of starch without separating amylose and amylopectin, and the result was compared with amylose-free waxy maize starch. the clusters in the whole starch were produced by partial hydrolysis using α-amylase of bacillus amyloliquefaciens, and were subsequently treated with β-amylase to remove the linear amylose and to produce β-limit dextrins of clusters from amylopectin. the clusters were further hydrolyzed extensively with α-amyla ... | 2013 | 23911473 |
| combined high pressure and thermal processing on inactivation of type a and proteolytic type b spores of clostridium botulinum. | the aim of this study was to determine the resistance of multiple strains of clostridium botulinum type a and proteolytic type b spores exposed to combined high pressure and thermal processing and compare their resistance with clostridium sporogenes pa3679 and bacillus amyloliquefaciens tmw-2.479-fad-82 spores. the resistance of spores suspended in n-(2acetamido)-2-aminoethanesulfonic acid (aces) buffer (0.05 m, ph 7.0) was determined at a process temperature of 105°c, with high pressures of 600 ... | 2013 | 23905794 |
| structural and functional insight into an unexpectedly selective n-methyltransferase involved in plantazolicin biosynthesis. | plantazolicin (pzn), a polyheterocyclic, n(α),n(α)-dimethylarginine-containing antibiotic, harbors remarkably specific bactericidal activity toward strains of bacillus anthracis, the causative agent of anthrax. previous studies demonstrated that genetic deletion of the s-adenosyl-l-methionine-dependent methyltransferase from the pzn biosynthetic gene cluster results in the formation of desmethylpzn, which is devoid of antibiotic activity. here we describe the in vitro reconstitution, mutational ... | 2013 | 23878226 |
| curing the plasmid pmc1 from the poly (γ-glutamic acid) producing bacillus amyloliquefaciens ll3 strain using plasmid incompatibility. | bacillus amyloliquefaciens ll3 is a glutamate-independent poly-γ-glutamic acid (γ-pga) producing strain which consists of a circular chromosome (3,995,227 bp) and an endogenous plasmid pmc1 (6,758 bp). the study of the function of native plasmid and the genome-size reduction of the b. amyloliquefaciens ll3 strain requires elimination of the endogenous plasmid. traditional plasmid-curing procedures using sodium dodecyl sulfate (sds) or acridine orange combined with heat treatment have been shown ... | 2013 | 23873640 |
| effect of metals on a siderophore producing bacterial isolate and its implications on microbial assisted bioremediation of metal contaminated soils. | a bacterial isolate producing siderophore under iron limiting conditions, was isolated from mangroves of goa. based on morphological, biochemical, chemotaxonomical and 16s rdna studies, the isolate was identified as bacillus amyloliquefaciens nar38.1. preliminary characterization of the siderophore indicated it to be catecholate type with dihydroxy benzoate as the core component. optimum siderophore production was observed at ph 7 in mineral salts medium (msm) without any added iron with glucose ... | 2013 | 23838040 |
| antitumor activity of bacterial exopolysaccharides from the endophyte bacillus amyloliquefaciens sp. isolated from ophiopogon japonicus. | the endophytic bacterium, md-b1, was isolated from the medicinal plant ophiopogon japonicas and identified as the bacillus amyloliquefaciens sp. with 99% similarity based on the partial sequence analysis of 16s rdna. exopolysaccharides were extracted from the endophyte for the evaluation of its antitumor activity against gastric carcinoma cell lines (mc-4 and sgc-7901). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assays and microscopy were performed to estimate the cell vi ... | 2013 | 23833642 |
| limited impact of abiotic stress on surfactin production in planta and on disease resistance induced by bacillus amyloliquefaciens s499 in tomato and bean. | understanding how temperature and water stress affect protocooperation between plants and beneficial rhizobacteria may enhance the efficacy of biocontrol agents in reducing plant diseases. however, little is known about the impact of these factors on biocontrol mechanisms and effectiveness, especially when provided by beneficial bacillus spp. this work aimed to evaluate the influence of low/high temperature combined with a normal and reduced water regime on the interaction between bacillus amylo ... | 2013 | 23829709 |
| isolation and characterization of a thermophilic bacillus sp. with protease activity isolated from hot spring of tarabalo, odisha, india. | thermophilic bacteria are less studied but important group of microorganisms due to their ability to produce industrial enzymes. | 2013 | 23825735 |
| purification and characterization of a new metallo-neutral protease for beer brewing from bacillus amyloliquefaciens syb-001. | the increased additive amount of adjuncts in the raw materials of chinese beer requires the usage of protease to release more water-soluble proteins. here, a metallo-neutral protease suited for brewing industry was purified from bacillus amyloliquefaciens syb-001. a 5.6-fold purification of the neutral protease was achieved with a 4-step procedure including ammonium sulfate precipitation, ion-exchange, hydrophobic interaction, and gel-filtration chromatography. the molecular mass of the enzyme w ... | 2013 | 23813340 |
| fermentation of biodiesel-derived glycerol by bacillus amyloliquefaciens: effects of co-substrates on 2,3-butanediol production. | cultivation in glycerol instead of sugars inhibits 2,3-butanediol (2,3-bd) production by bacillus amyloliquefaciens. in this study, we report that b. amyloliquefaciens readily produces 2,3-bd from biodiesel-derived glycerol in the presence of beet molasses as a co-substrate. unexpectedly, the molasses stimulated 2,3-bd production and simultaneously reduced the duration of fermentation. productivity of 2,3-bd was enhanced at the start of fermentation, and yields increased under continuous molasse ... | 2013 | 23797331 |
| induction of apoptosis in human cancer cells by a bacillus lipopeptide bacillomycin d. | a newly isolated and characterized bacillus amyloliquefaciens strain fiply 3a has been found to produce an extracellular cyclic lipopeptide which structurally resembled bacillomycin d, earlier reported to be produced by bacillus subtilis. the lipopeptide showed a dose dependent killing of three different human cancer cell lines viz. a549 (alveolar adenocarcinoma), a498 (renal carcinoma) and hct-15 (colon adenocarcinoma), while not affecting the normal cell line l-132 (pulmonary epithelial cells) ... | 2013 | 23770444 |
| isolation, identification, and characterization of a cellulolytic bacillus amyloliquefaciens strain ss35 from rhinoceros dung. | cellulose hydrolyzing bacteria were isolated from rhinoceros dung and tested for clear zone formation around the colonies on the agar plates containing the medium amended with carboxymethylcellulose as a sole carbon source. isolates were further screened on the basis of carboxymethylcellulase production in liquid medium. out of 36 isolates, isolate no. 35 exhibited maximum enzyme activity of 0.079 u/ml and was selected for further identification by using conventional biochemical tests and phylog ... | 2013 | 23762763 |
| total synthesis of the ribosomally synthesized linear azole-containing peptide plantazolicin a from bacillus amyloliquefaciens. | | 2013 | 23761292 |
| new insights into membrane-active action in plasma membrane of fungal hyphae by the lipopeptide antibiotic bacillomycin l. | bacillomycin l, a natural iturinic lipopeptide produced by bacillus amyloliquefaciens, is characterized by strong antifungal activities against a variety of agronomically important filamentous fungi including rhizoctonia solani kühn. prior to this study, the role of membrane permeabilization in the antimicrobial activity of bacillomycin l against plant pathogenic fungi had not been investigated. to shed light on the mechanism of this antifungal activity, the permeabilization of r. solani hyphae ... | 2013 | 23756779 |
| site-directed mutagenesis studies on the uridine monophosphate binding sites of feedback inhibition in carbamoyl phosphate synthetase and effects on cytidine production by bacillus amyloliquefaciens. | a major problem when pyrimidine de novo biosynthesis is used for cytidine production is the existence of many negative regulatory factors. cytidine biosynthesis in bacillus amyloliquefaciens proceeds via a pathway that is controlled by uridine monophosphate (ump) through feedback inhibition of carbamoyl phosphate synthetase (cps), the enzyme that converts co2, nh3, and glutamine to carbamoyl phosphate. in this study, the gene carb encoding the large subunit of cps from b. amyloliquefaciens cyt1 ... | 2013 | 23750951 |
| antifungal activity of the lipopeptides produced by bacillus amyloliquefaciens anti-ca against candida albicans isolated from clinic. | the bacterium bacillus amyloliquefaciens anti-ca isolated from mangrove system was found to be able to actively kill candida albicans isolated from clinic. the bacterial strain anti-ca could produce high level of bioactive substance, amylase and protease in the cheap medium containing 2.0 % soybean meal, 2.0 % wheat flour, ph 6.5 within 26 h. after purification, the main bioactive substance was confirmed to be a cyclic lipopeptide containing a heptapeptide, l-asp→l-leu→l-leu→l-val→l-val→l-glu→l- ... | 2013 | 23744287 |
| characterization of a fibrinolytic enzyme secreted by bacillus amyloliquefaciens cb1 and its gene cloning. | bacillus amyloliquefaciens cb1 was isolated from cheonggukjang, a korean fermented soy food. b. amyloliquefaciens cb1 secretes proteases with fibrinolytic activities. a gene homologous to apre of bacillus subtilis, aprecb1, was cloned from b. amyloliquefaciens cb1, and dna sequencing showed that aprecb1 can encode a prepro-type serine protease consisting of 382 amino acids. when aprecb1 was introduced into b. subtilis wb600 using an e. coli-bacillus shuttle vector, phy300plk, transformants showe ... | 2013 | 23727811 |
| biological control of apple ring rot on fruit by bacillus amyloliquefaciens 9001. | apple ring rot disease, caused by botryosphaeria dothidea (moug. ex. fr) ces. et de not., is one of the most important diseases on apple fruits. in this study, strain 9001 isolated from healthy apple fruits from an infested orchard was evaluated for its biocontrol activity against apple ring rot in vitro and in vivo. strain 9001 showed obvious antagonistic activity to b. dothidea yl-1 when plated on potato dextrose agar. soaking healthy apples in the bacterial suspensions of strain 9001 prior to ... | 2013 | 25288943 |
| [screening and identification of low temperature-adapted antagonistic bacillus isolated from kekexili region of west china and the analysis of the isolates lipopeptide compounds]. | the research and exploitation of special microbial resources in extreme environment is of scientific significance and has broad applied prospect. in this paper, eight bacillus strains isolated from the vegetation rhizospheres in kekexili extreme region of qinghai province and presented good growth status at low temperature 4 and 10 degrees c were identified. through physiological and biochemical analysis, rep-pcr fingerprinting, and 16s rdna and gyrb partial sequence analyses, the eight strains ... | 2013 | 23718003 |
| the usage of rice straw as a major substrate for the production of surfactin by bacillus amyloliquefaciens xz-173 in solid-state fermentation. | agro-industrial byproducts, especially rice straw, are potential resources. this work was aimed to utilize raw materials to produce value-added biosurfactant in solid-state fermentation (ssf). rice straw and soybean flour were found efficient and selected as major substrates for surfactin production. the results of plackett-burman design indicated that glycerol, water content, inoculum size and temperature were the significant variables identified in the screen of nine total variables. the optim ... | 2013 | 23685270 |
| statistical optimization of medium components for milk-clotting enzyme production by bacillus amyloliquefaciens d4 using wheat bran-an agro-industry waste. | in this paper, two statistical methods were applied to optimize medium components to improve the production of the milk-clotting enzyme by bacillus amyloliquefaciens d4. first, wheat bran juice, skim milk powder, and na2hpo4 were shown to have significant effects on d4 enzyme production using the plackett-burman experimental design. subsequently, an optimal medium was obtained using the box-behnken method, which consisted of 3.31 g/l of skim milk powder, 5.0 g/l of sucrose, 0.1 g/l of feso4·7h2o ... | 2013 | 23676905 |
| comparative study on the properties of lipopeptide products and expression of biosynthetic genes from bacillus amyloliquefaciens xz-173 in liquid fermentation and solid-state fermentation. | this study compared the compositions and properties of lipopeptide products purified from liquid fermentation (lf) and solid-state fermentation (ssf) and transcription levels involved in lipopeptides under the two systems. results of fourier transform infrared spectroscopy and high performance liquid chromatography revealed no significant differences in the polarity and structure of the two lipopeptide products. a higher amino acid proportion was found for lipopeptide product in lf than in ssf. ... | 2013 | 23670314 |
| safety, formulation, and in vitro antiviral activity of the antimicrobial peptide subtilosin against herpes simplex virus type 1. | in the present study the antiviral properties of the bacteriocin subtilosin against herpes simplex virus type 1 (hsv-1) and the safety and efficacy of a subtilosin-based nanofiber formulation were determined. high concentrations of subtilosin, the cyclical antimicrobial peptide produced by bacillus amyloliquefaciens, were virucidal against hsv-1. interestingly, at non-virucidal concentrations, subtilosin inhibited wild type hsv-1 and aciclovir-resistant mutants in a dose-dependent manner. althou ... | 2013 | 23637711 |
| production and partial characterization of extracellular amylase enzyme from bacillus amyloliquefaciens p-001. | amylases are one of the most important enzymes in present-day biotechnology. the present study was concerned with the production and partial characterization of extracellular amylase from bacillus amyloliquefaciens p-001. the effect of various fermentation conditions on amylase production through shake-flask culture was investigated. enzyme production was induced by a variety of starchy substrate but corn flour was found to be a suitable natural source for maximum production. tryptone and ammoni ... | 2013 | 23626928 |
| wh1fungin a surfactin cyclic lipopeptide is a novel oral immunoadjuvant. | wh1fungin, a surfactin lipopeptide from bacillus amyloliquefaciens wh1, can be used as an adjuvant for eliciting strong immune response by parenteral immunization. in this study, wh1fungin was firstly reported as an oral immunoadjuvant. in mice, wh1fungin markedly enhanced the immune response to co-administered protein antigens (ova or gst), similar to levels elicited by ctb, but no immune response was elicited to itself. both igg1 and igg2a antibodies elicited from the immunizations indicating ... | 2013 | 23624094 |
| using bacillus amyloliquefaciens for remediation of aquaculture water. | remediation of aquaculture water using microorganisms like bacillus species is a burgeoning trend for the sustainable development of aquaculture industries. in this work, a bacillus amyloliquefaciens strain (namely b. amyloliquefaciens hn), isolated from activated sludge of a polluted river, was evaluated for its potential in water remediation using simulated aquaculture water. b. amyloliquefaciens hn exhibited high tolerance towards 80 mg l(-1) of nitrite-n and ammonia-n. it could effectively r ... | 2013 | 23577299 |
| bacillus amyloliquefaciens ssp. plantarum strains as potential protective starter cultures for the production of bikalga, an alkaline fermented food. | to identify and screen dominant bacillus spp. strains isolated from bikalga, fermented seeds of hibiscus sabdariffa for their antimicrobial activities in brain heart infusion (bhi) medium and in a h. sabdariffa seed-based medium. further, to characterize the antimicrobial substances produced. | 2013 | 23565829 |
| bacillus amyloliquefaciens q-426 as a potential biocontrol agent against fusarium oxysporum f. sp. spinaciae. | in recent years, bacillus species have received considerable attention for the biological control of many fungal diseases. in this study, bacillus amyloliquefaciens q-426 was tested for its potential use against a variety of plant pathogens. our screen for genes involved in the biosynthesis of antifungal agents revealed that the fen and bmy gene clusters are present in the q-426 genome. lipopeptides such as bacillomycin d, fengycin a, and fengycin b were purified from the bacterial culture broth ... | 2014 | 23553741 |
| plant growth-promoting rhizobacteria strain bacillus amyloliquefaciens njn-6-enriched bio-organic fertilizer suppressed fusarium wilt and promoted the growth of banana plants. | bacillus amyloliquefaciens strain njn-6 is an important plant growth-promoting rhizobacteria (pgpr) which can produce secondary metabolites antagonistic to several soil-borne pathogens. in this study, the ability of a bio-organic fertilizer (bio) containing njn-6 strain to promote the growth and suppress fusarium wilt of banana plants was evaluated in a pot experiment. the results showed that the application of bio significantly decreased the incidence of fusarium wilt and promoted the growth of ... | 2013 | 23541032 |
| chromosome integration of the vitreoscilla hemoglobin gene (vgb) mediated by temperature-sensitive plasmid enhances γ-pga production in bacillus amyloliquefaciens. | oxygen is a limiting factor in the production of γ-pga by the glutamic acid-independent strain bacillus amyloliquefaciens ll3 because of the high viscosity of the culture broth. the vgb gene encoding vitreoscilla hemoglobin (vhb) was introduced into ll3 to overcome the low concentration of dissolved oxygen (do). first, recombinant plasmid pwhv was constructed by cloning vgb into the bacillus expression vector pwh1520 and transformed into ll3. carbon monoxide difference spectral analysis confirme ... | 2013 | 23521121 |
| antagonistic bacterium bacillus amyloliquefaciens induces resistance and controls the bacterial wilt of tomato. | bacterial wilt caused by ralstonia solanacearum (rs) is a serious threat for agricultural production. in this study, bacillus amyloliquefaciens strains cm-2 and t-5 antagonistic to rs were used to create bioorganic fertilisers to control tomato wilt under greenhouse conditions. the possible mechanism of resistance inducement by the antagonistic bacteria was also evaluated. | 2013 | 23519834 |
| genome sequence of a plant-associated bacterium, bacillus amyloliquefaciens strain ucmb5036. | we announce here the genome sequence of bacillus amyloliquefaciens strain ucmb5036, a plant growth-promoting bacterium isolated from a cotton plant. its genome contains gene clusters involved in nonribosomal synthesis of secondary metabolites known for their antimicrobial activities. the availability of this genome will provide novel insights into plant-bacterium-associated activities. | 2013 | 23516223 |
| identification of up-regulated proteins potentially involved in the antagonism mechanism of bacillus amyloliquefaciens g1. | the use of bacillus probiotics has been demonstrated as a promising method in the biocontrol of bacterial diseases in aquaculture. however, the molecular antibacterial mechanism of bacillus still remains unclear. in order to explore the antibacterial mechanism of the potential antagonistic bacillus amyloliquefaciens strain g1, comparative proteomics between b. amyloliquefaciens strain g1 and its non-antagonistic mutant strain was investigated. the 2-dimensional electrophoresis gel maps of their ... | 2013 | 23483288 |
| probiotic bacillus amyloliquefaciens mediate m1 macrophage polarization in mouse bone marrow-derived macrophages. | depending on the microenvironment, macrophages can acquire distinct functional phenotypes, referred to as classically activated m1 and m2. m1 macrophages are considered potent effector cells that kill intracellular pathogens, and m2 macrophages promote the resolution of wound healing. in this study, we are interested to know whether probiotic bacillus amyloliquefaciens (ba) can induce macrophages polarization. real-time fluorescence pcr analysis demonstrated that the expression of il-1β, inos, t ... | 2013 | 23455449 |
| plant growth-promoting bacteria bacillus amyloliquefaciens nbrisn13 modulates gene expression profile of leaf and rhizosphere community in rice during salt stress. | growth and productivity of rice and soil inhabiting microbial population is negatively affected by soil salinity. however, some salt resistant, rhizosphere competent bacteria improve plant health in saline stress. present study evaluated the effect of salt tolerant bacillus amyloliquefaciens nbrisn13 (sn13) inoculation on rice plants in hydroponic and soil conditions exposed to salinity. sn13 increased plant growth and salt tolerance (nacl 200 mm) and expression of at least 14 genes under hydrop ... | 2013 | 23454292 |
| isolation of the putative biosynthetic gene cluster of 1-deoxynojirimycin by bacillus amyloliquefaciens 140n, its production and application to the fermentation of soybean paste. | the 1-deoxynojirimycin (dnj) biosynthetic gene cluster of bacillus amyloliquefaciens 140n isolated from traditional korean fermented food was isolated by pcr screening. it showed 78.9% inhibitory activity against α-glucosidase and produced 0.8 g/l of dnj in an optimized medium containing 2% soluble starch, 1% tryptone, 0.05% kh(2)po(4), and 0.05% (nh(2))(4)so(4). soybean paste fermented with b. amyloliquefaciens 140n produced dnj with 84.4% inhibitory activity. | 2013 | 23391926 |
| characterization of fungal antagonistic bacilli isolated from aerial roots of banyan (ficus benghalensis) using intact-cell maldi-tof mass spectrometry (icms). | to characterize fungal antagonistic bacilli isolated from aerial roots of banyan tree and identify the metabolites responsible for their antifungal activity. | 2013 | 23387377 |
| effects of critical medium components on the production of antifungal lipopeptides from bacillus amyloliquefaciens q-426 exhibiting excellent biosurfactant properties. | in this study, influence of three critical parameters nitrogen sources, initial ph and metal ions was discussed in the production of antifungal lipopeptides from bacillus amyloliquefaciens q-426. the results revealed that lipopeptide biosynthesis might have relations with the population density of strain q-426 and some special amino acids. also, the alkali-resistant strain q-426 could grow well in the presence of fe(2+) ions below 0.8 m l(-1) and still maintain the competitive advantage below 0. ... | 2013 | 23329061 |
| role of the salt bridge between arg176 and glu126 in the thermal stability of the bacillus amyloliquefaciens α-amylase (baa). | in the bacillus amyloliquefaciens α-amylase (baa), the loop (residues 176-185; region i) that is the part of the calcium-binding site (cai, ii) has two more amino acid residues than the α-amylase from bacillus licheniformis (bla). arg176 in this region makes an ionic interaction with glu126 from region ii (residues 118-130), but this interaction is lost in bla owing to substitution of r176q and e126v. the goal of the present work was to quantitatively estimate the effect of ionic interaction on ... | 2013 | 23314361 |
| acetoin production enhanced by manipulating carbon flux in a newly isolated bacillus amyloliquefaciens. | a new strain, fmme044, exhibited a remarkable ability to synthesize acetoin and was identified as bacillus amyloliquefaciens. the following characteristics of enzyme activity were found: 2,3-butanediol was reverse transformed to acetoin upon depletion of glucose; lower agitation speeds favored 2,3-butanediol accumulation; and higher agitation speeds favored reverse transformation of 2,3-butanediol to acetoin. in order to enhance acetoin production by manipulating the carbon flux distribution, a ... | 2013 | 23306133 |
| development of a specific real-time pcr assay targeting the poly-γ-glutamic acid synthesis gene, pgsb, for the quantification of bacillus amyloliquefaciens in solid-state fermentation. | a taqman real-time pcr procedure was developed for specific detection and quantification of strains belonging to bacillus amyloliquefaciens group. the primer pair pgsb726-f/pgsb791-r and the pgsb-probe were designed from one of the poly-γ-glutamic acid synthesis gene (pgsb) of b. amyloliquefaciens. the detection limit was approximately between 10(2)-10(3) cells/ml. a linear correlation between the log10 input pmd-pgsb plasmid dna copies and the threshold cycle values were observed with a magnitu ... | 2013 | 23266849 |
| preparative isolation and purification of macrolactin antibiotics from marine bacterium bacillus amyloliquefaciens using high-speed counter-current chromatography in stepwise elution mode. | preparative high-speed counter-current chromatography (hsccc) was successfully applied to the isolation and purification of two macrolactin antibiotics from marine bacterium bacillus amyloliquefaciens for the first time using stepwise elution with a pair of two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water at (1:4:1:4, v/v) and (3:4:3:4, v/v). the preparative hsccc separation was performed on 300 mg of crude sample yielding macrolactin b (22.7 mg) and macrolactin a (40. ... | 2013 | 23253117 |
| [isolation, identification and cellulase production of a cellulolytic bacterium from intestines of giant panda]. | to isolate and identify the cellulose-producing bacterium from fresh feces of healthy giant pandas, and characterize its cellulase production. | 2012 | 23236845 |
| draft genome sequence of the biocontrol bacterium bacillus amyloliquefaciens strain m27. | bacillus amyloliquefaciens strain m27 is a biocontrol agent with antagonistic activities against a wide range of fungal pathogens. here we present the 3.86-mb draft genome sequence of the bacterium with the aims of providing insights into the genomic basis of its antifungal mechanism and facilitating its application in the biocontrol of plant diseases. | 2012 | 23209201 |
| characterisation and profiling of bacillus subtilis, bacillus cereus and bacillus licheniformis by maldi-tof mass fingerprinting. | the bacillus genus includes species such as bacillus cereus, bacillus licheniformis and bacillus subtilis, some of which may be pathogenic or causative agents in the spoilage of food products. the main goal of this work was to apply matrix-assisted laser desorption ionisation-time of flight (maldi-tof) mass fingerprinting to the classification of these bacillus species. genetic analyses were also compared to phyloproteomic analyses. a collection of 57 bacillus strains isolated from fresh and pro ... | 2013 | 23200657 |
| enhanced root colonization and biocontrol activity of bacillus amyloliquefaciens sqr9 by abrb gene disruption. | root colonization by antagonistic bacteria is a prerequisite for successful biological control, and the instability of colonization under varying environmental conditions has accentuated the need to improve the colonization activity. root colonization by bacillus spp. is mainly determined by chemotaxis and biofilm formation, and both functions are negatively controlled by the global transcription regulator abrb. here, we disrupted the gene abrb in bacillus amyloliquefaciens sqr9, which has been ... | 2013 | 23196984 |
| industrial waste based compost as a source of novel cellulolytic strains and enzymes. | ninety bacteria isolated from raw composting materials were screened for their cellulolytic activity on solid medium containing carboxymethylcellulose. the bacteria producing the highest cellulolytic activity levels were identified by 16s rrna sequencing as bacillus licheniformis strain 1, bacillus subtilis subsp. subtilis strain b7b, bacillus subtilis subsp. spizizenii strain 6, and bacillus amyloliquefaciens strain b31c. cellulase activity production by the most productive strain b. amylolique ... | 2013 | 23181595 |
| effect of silver nanoparticles on oryza sativa l. and its rhizosphere bacteria. | silver nanoparticles (agnps) are widely used as antibacterial and antifungal agents in agriculture. nevertheless, these nanoparticles with newborn properties pose a potential risk to the environment, due to contact with crops and bacteria that are beneficial to the soil. this study is based on the examination of the phytotoxic effects of agnps on oryza sativa l. and some of its rhizosphere bacteria, by physiological and biochemical assays. in order to study the complex interaction of the agnps l ... | 2013 | 23174269 |
| contribution of bacillomycin d in bacillus amyloliquefaciens sqr9 to antifungal activity and biofilm formation. | bacillus amyloliquefaciens strains are capable of suppressing soilborne pathogens through the secretion of an array of lipopeptides and root colonization, and biofilm formation ability is considered a prerequisite for efficient root colonization. in this study, we report that one of the lipopeptide compounds (bacillomycin d) produced by the rhizosphere strain bacillus amyloliquefaciens sqr9 not only plays a vital role in the antagonistic activity against fusarium oxysporum but also affects the e ... | 2013 | 23160135 |
| in silico characterization of a novel β-1,3-glucanase gene from bacillus amyloliquefaciens--a bacterial endophyte of hevea brasiliensis antagonistic to phytophthora meadii. | we report the molecular characterization of β-1,3-glucanase-producing bacillus amyloliquefaciens-an endophyte of hevea brasiliensis antagonistic to phytophthora meadii. after cloning and sequencing, the β-1,3-glucanase gene was found to be 747 bp in length. a homology model of the β-1,3-glucanase protein was built from the amino acid sequence obtained upon translation of the gene. the target β-1,3-glucanase protein and the template protein, endo β-1,3-1,4-glucanase protein (pdb id: 3o5s), were f ... | 2013 | 23108702 |
| bioconversion of p-coumaric acid to p-hydroxystyrene using phenolic acid decarboxylase from b. amyloliquefaciens in biphasic reaction system. | phenolic acid decarboxylase (pad) catalyzes the non-oxidative decarboxylation of p-coumaric acid (pca) to p-hydroxystyrene (phs). pad from bacillus amyloliquefaciens (bapad), which showed k (cat)/k (m) value for pca (9.3 × 10³ mm⁻¹ s⁻¹), was found as the most active one using the "subgrouping automata" program and by comparing enzyme activity. however, the production of phs of recombinant escherichia coli harboring bapad showed only a 22.7 % conversion yield due to product inhibition. based on t ... | 2013 | 23081771 |
| iturin levels on wheat spikes linked to biological control of fusarium head blight by bacillus amyloliquefaciens. | the trigocor strain of bacillus amyloliquefaciens provides consistent control against fusarium head blight of wheat in controlled settings but there is a lack of disease and deoxynivalenol suppression in field settings. since production of antifungal compounds is thought to be the main mode of action of trigocor control, we quantified levels of a key family of antifungal metabolites, iturins, as well as monitored bacillus populations on wheat spikes over 14 days post-application in both the gree ... | 2013 | 23075168 |
| fast and efficient generation of recombinant baculoviruses by in vitro transposition. | a novel recombinant bacmid, beasybac, that enables the easy and fast generation of pure recombinant baculovirus without any purification step was constructed. in beasybac, attr recombination sites were introduced to facilitate the generation of a recombinant viral genome by in vitro transposition. moreover, the extracellular rnase gene from bacillus amyloliquefaciens, barnase, was expressed under the control of the cotesia plutellae bracovirus early promoter to negatively select against the non- ... | 2012 | 23053112 |
| modeling antimicrobial activity of lipopeptides from bacillus amyloliquefaciens es-2 against shewanella putrefaciens in shrimp meat using a response surface method. | bacillus amyloliquefaciens es-2 can produce antimicrobial lipopeptides, including surfactin and fengycin. in this study, the model of antimicrobial activity against shewanella putrefaciens in shrimp meat by antimicrobial lipopeptides from b. amyloliquefaciens es-2 was researched by response surface methodology. the results showed that s. putrefaciens had high sensitivity to antimicrobial lipopeptides, which had a mic of 0.6 mg/ml. a quadratic mathematical model representative of the action of an ... | 2012 | 23043837 |
| a surfactin cyclopeptide of wh1fungin used as a novel adjuvant for intramuscular and subcutaneous immunization in mice. | wh1fungin, a surfactin cyclopeptide from bacillus amyloliquefaciens wh1, is firstly reported as a novel immunoadjuvant, which can markedly enhance the immune response when given in mixture with antigens. after intramuscular or subcutaneous immunization, wh1fungin can help to induce both of durable humoral and cellular immune response, even as strong as freund's adjuvant. both igg1 and igg2a antigen-specific antibodies were elicited from the immunizations indicating a mixed th1/th2 response. sple ... | 2012 | 22982610 |
| chitin extraction from shrimp shell waste using bacillus bacteria. | the ability of six protease-producing bacillus species (bacillus pumilus a1, bacillus mojavencis a21, bacillus licheniformis rp1, bacillus cereus sv1, bacillus amyloliquefaciens an6 and bacillus subtilis a26) to ferment media containing only shrimp shell waste, for chitin extraction, was investigated. more than 80% deproteinization was attained by all the strains tested. however, demineralization rates not exceeding 67% were registered. cultures conducted in media containing shrimp shell waste s ... | 2012 | 22981824 |
| immobilization of the α-amylase of bacillus amyloliquifaciens tswk1-1 for the improved biocatalytic properties and solvent tolerance. | the α-amylase of bacillus amyloliquifaciens tswk1-1 (genbank number, gq121033) was immobilized by various methods, including ionic binding with deae cellulose, covalent coupling with gelatin and entrapment in polyacrylamide and agar. the immobilization of the purified enzyme was most effective with the deae cellulose followed by gelatin, agar and polyacrylamide. the k m increased, while v max decreased upon immobilization on various supports. the temperature and ph profiles broadened, while ther ... | 2013 | 22961428 |
| [effects of overexpression of key enzyme genes on guanosine accumulation in bacillus amyloliquefaciens]. | to study the effects of overexpression of key enzyme genes (prs, purf and guab) on guanosine production in bacillus amyloliquefaciens ta208. | 2012 | 22934352 |
| characterizing a novel strain of bacillus amyloliquefaciens bac03 for potential biological control application. | to identify and characterize a bacterial strain bac03, evaluate its biological control activity against potato common scab (streptomyces spp.) and characterize an antimicrobial substance produced by bac03. | 2012 | 22924833 |
| isolation and molecular characterization of a novel strain of bacillus with antifungal activity from the sorghum rhizosphere. | we looked for bacterial strains with antifungal activity in the sorghum rhizosphere. a prescreening procedure to search for hemolytic activity among the isolated strains allowed us to detect good fungitoxic activity in a bacterial isolate that we named um96. this bacterial isolate showed strong growth inhibition in bioassays against the pathogens diaporthe phaseolorum, colletotrichum acutatum, rhizoctonia solani, and fusarium oxysporum. the supernatant of isolate um96 also showed strong hemolyti ... | 2012 | 22869080 |
| optimization and scale-up of 2,3-butanediol production by bacillus amyloliquefaciens b10-127. | the effects of culture conditions on 2,3-butanediol (2,3-bd) production and its possible scale-up have been studied. a newly isolated bacillus amyloliquefaciens b10-127, belonged to gras microorganisms and showed a remarkable 2,3-bd producing potency, was used for this experiment. corn steep liquor, soybean meal and ammonium citrate were found to be the key factors in the fermentation according to the results obtained from the plackett-burman experimental design. the optimal concentration range ... | 2012 | 22805938 |
| characterization and application of calcium-dependent β-propeller phytase from bacillus amyloliquefaciens ds11. | the enzyme phytase has broad biotechnological applications, especially in the reduction of phytate, antinutritional factors that chelate essential minerals, in human and animal food. we investigated the enzymatic properties of β-propeller phytase (bpp) from bacillus amyloliquefaciens ds11. thermal refolding analysis demonstrated that bpp can remarkably restore its enzymatic activity in the presence of 5 mm ca(2+) to 87% of its original activity after heating to 100 °c and subsequent cooling, ind ... | 2012 | 22775008 |
| antifungal activity of bacillus amyloliquefaciens njn-6 volatile compounds against fusarium oxysporum f. sp. cubense. | bacillus amyloliquefaciens njn-6 produces volatile compounds (vocs) that inhibit the growth and spore germination of fusarium oxysporum f. sp. cubense. among the total of 36 volatile compounds detected, 11 compounds completely inhibited fungal growth. the antifungal activity of these compounds suggested that vocs can play important roles over short and long distances in the suppression of fusarium oxysporum. | 2012 | 22685147 |
| identification of the bacteriocin subtilosin a and loss of purl results in its high-level production in bacillus amyloliquefaciens. | a mini-tn10 transposon mutagenesis library of strain bacillus amyloliquefaciens bahja nk10 was constructed and one mutant, l4, exhibiting markedly enhanced antagonistic activity against micrococcus luteus, was screened and possessed an inactivated purl gene. a similar antibacterial spectrum was observed for both l4 and wild-type strains, but l4 displayed a markedly strengthened antimicrobial effect. extracts of the corresponding culture media were separated by means of liquid chromatography and ... | 2012 | 22677773 |
| evaluation of indigenous bacterial strains for biocontrol of the frogeye leaf spot of soya bean caused by cercospora sojina. | assessment of biological control of cercospora sojina, causal agent of frogeye leaf spot (fls) of soya bean, using three indigenous bacterial strains, bnm297 (pseudomonas fluorescens), bnm340 and bnm122 (bacillus amyloliquefaciens). | 2012 | 22671984 |
| optimization for the production of surfactin with a new synergistic antifungal activity. | two of our long term efforts are to discover compounds with synergistic antifungal activity from metabolites of marine derived microbes and to optimize the production of the interesting compounds produced by microorganisms. in this respect, new applications or mechanisms of already known compounds with a high production yield could be continually identified. surfactin is a well-known lipopeptide biosurfactant with a broad spectrum of antimicrobial and antiviral activity; however, there is less k ... | 2012 | 22629294 |
| [analysis of microorganism species diversity in plant intercropping models in a wetland system constructed for treatment of municipal sewage]. | the selective culture method and pcr-dgge technology were applied to analyze the number and the biodiversity of microorganism species in cells with plant intercropping models and without plants in different seasons in a wetland system constructed for treatment of municipal sewage. the results showed that the numbers of microorganisms were considerably larger in the cells with plant intercropping models than those without plants, while the number of microorganisms was apparently larger in summer ... | 2011 | 22619969 |
| the ultrasound-assisted extraction and identification of antifungal substances from b. amyloliquefaciens strain njn-6 suppressing fusarium oxysporum. | the primary mechanism underlying antagonism among microorganisms is the production of antagonistic substances called antibiotics that inhibit the growth of pathogens. in this study, the antagonistic substances produced by the bacillus amyloliquefaciens strain njn-6 that had antifungal activity against fusarium oxysporum were extracted and identified. the active antifungal substance was extracted from dried leavening with ultrasound-assisted extraction (uae), using n -butanol as the extractant. h ... | 2012 | 22581589 |
| improvement of cellulase activity using error-prone rolling circle amplification and site-directed mutagenesis. | improvement of endoglucanase activity was accomplished by utilizing error-prone rolling circle amplification, supplemented with 1.7 mm mncl2. this procedure generated random mutations in the bacillus amyloliquefaciens endoglucanase gene with a frequency of 10 mutations per kilobase. six mutated endoglucanase genes, recovered from six colonies, possessed endoglucanase activity between 2.50- and 3.12-folds higher than wild type. we sequenced these mutants, and the different mutated sites of nucleo ... | 2012 | 22561853 |
| investigation on the effects of three x-->histidine replacements on thermostability of alpha-amylase from bacillus amyloliquefaciens. | bacillus licheniformis alpha-amylase (bla), a thermophilic counterpart of bacillus amyloliquefaciens alpha-amylase (baa), is an appropriate model for the design of stabilizing mutations in baa. bla has 10 more histidines than baa. considering this prominent difference, in the present study, three out of these positions (i34, q67, and p407; located in the thermostability determinant 1 region and ca-iii binding site of baa) were replaced with histidine in baa, using the site-directed mutagenesis t ... | 2012 | 22561851 |
| diversity of spore-forming bacteria and identification of bacillus amyloliquefaciens as a species frequently associated with the ropy spoilage of bread. | this study examines the diversity of spore-forming bacteria isolated from raw materials/bread using molecular methods along with a rapid and innovative technology, the ft-nir spectroscopy. microbiological analysis showed that 23% of semolina and 42% of other raw materials (including grain, brewer yeast, improvers) contained more than 100 spores/g and more than 50% of each kind of sample was contaminated at a level ranging from 1 to 100 spores/g. a high bacterial diversity characterized raw mater ... | 2012 | 22551674 |
| application of fusion protein 4d5 scfv-dibarnase:barstar-gold complex for studying p185her2 receptor distribution in human cancer cells. | overexpression of the p185(her2) protein determines the malignancy and unfavorable prognosis of ovarian and breast tumors. in this work, the distribution of p185(her2) in human cancer cells was studied by electron microscopy, using a novel approach. it is based on the interaction between barnase (a ribonuclease from bacillus amyloliquefaciens) and its specific inhibitor barstar. the monoclonal antibody 4d5 scfv to extracellular p185(her2) domain fused with two molecules of barnase was used as a ... | 2012 | 22531627 |
| study of the anti-sapstain fungus activity of bacillus amyloliquefaciens cgmcc 5569 associated with ginkgo biloba and identification of its active components. | an endophytic bacterium, designated strain bacillus amyloliquefaciens cgmcc 5569 was isolated from chinese medicinal ginkgo biloba collected from xuzhou, china. both the filtrate and the ethyl acetate extract of strain cgmcc 5569 showed growth inhibition activity against the sapstain fungi lasiodiplodia rubropurpurea, l. crassispora, and l. theobromae obviously (>65%) based on the comparison of the length of zones on the petri dish. from the ethyl acetate extract of the filtrate, the antifungal ... | 2012 | 22520222 |
| medium optimization of antifungal activity production by bacillus amyloliquefaciens using statistical experimental design. | in order to overproduce biofungicides agents by bacillus amyloliquefaciens blb371, a suitable culture medium was optimized using response surface methodology. plackett-burman design and central composite design were employed for experimental design and analysis of the results. peptone, sucrose, and yeast extract were found to significantly influence antifungal activity production and their optimal concentrations were, respectively, 20 g/l, 25 g/l, and 4.5 g/l. the corresponding biofungicide prod ... | 2012 | 22509851 |
| use of two bacteria for biological control of bayoud disease caused by fusarium oxysporum in date palm (phoenix dactylifera l) seedlings. | the bayoud, caused by fusarium oxysporum f. sp. albedinis (foa), is the most destructive disease of date palm (phoenix dactylifera l) in morocco and algeria, with no effective control strategy yet available. in this work, two bacteria, bacillus amyloliquefaciens strain ag1 (ag) and burkholderia cepacia strain cs5 (cs), were examined for their potential to control this disease. both bacterial strains inhibited both growth and sporulation of foa. they released compounds into the culture medium, wh ... | 2012 | 22480991 |
| metabolites of lactobacillus plantarum 2142 prevent oxidative stress-induced overexpression of proinflammatory cytokines in ipec-j2 cell line. | probiotics have already proven beneficial effects in the treatment of several intestinal infections, but the underlying mechanisms how the probiotics can affect responses of porcine ipec-j2 enterocytes to oxidative stress remained to be elucidated. the immunomodulatory effect of five bacterial strains (lactobacillus plantarum 2142, lactobacillus casei shirota, bifidobacterium animalis subsp. lactis bb-12, bacillus amyloliquefaciens cect 5940 and enterococcus faecium cect 4515) on 1 mm peroxide-t ... | 2012 | 22476971 |
| comment on enhancement of the catalytic activity of a 27 kda subtilisin-like enzyme from bacillus amyloliquefaciens ch51 by in vitro mutagenesis. | | 2012 | 22475247 |
| 16s rrna gene sequences analysis of ficus elastica rubber latex degrading thermophilic bacillus strain asu7 isolated from egypt. | a thermophilic bacillus strain asu7 was isolated from soil sample collected from assiut governorate in upper egypt on latex rubber-containing medium at 45 °c. genetically, the 16s bacterial ribosomal rna gene of the strain asu7 was amplified by the polymerase chain reaction (pcr) and sequenced. the sequence of the pcr product was compared with known 16s rrna gene sequences in the genbank database. based on phylogenetic analyses, strain asu7 was identified as bacillus amyloliquefaciens. the strai ... | 2012 | 22453659 |
| production of lipopeptides by bacillus amyloliquefaciens xz-173 in solid state fermentation using soybean flour and rice straw as the substrate. | this work was aimed to produce lipopeptides by bacillus amyloliquefaciens xz-173 in solid state fermentation using agro-industrial byproducts. a central composite design was used to get the highest lipopeptides production. results revealed that the optimal conditions for maximum lipopeptides production were 1.79% starch and 1.91% yeast extract by employing 5.58 g soybean flour and 3.67 g rice straw as the solid substrate with initial ph 7.5, moisture content 55% and a 10% inoculum level at 30°c ... | 2012 | 22418084 |
| efficacy of protected sodium butyrate, a protected blend of essential oils, their combination, and bacillus amyloliquefaciens spore suspension against artificially induced necrotic enteritis in broilers. | necrotic enteritis caused by clostridium perfringens leads to serious economical losses to the poultry industry. there is a growing need to find effective, nontoxic, antibiotic alternatives to prevent and cure the disease. in our study, the efficacy of protected sodium butyrate at 1.5 g/kg (bp70), a bacillus amyloliquefaciens spore suspension with 10(9) cfu/g (bal; ecobiol), a protected blend of essential oils (1%) at 1.5 g/kg (eo), and a combination of sodium butyrate with essential oils (1%) p ... | 2012 | 22399722 |
| production of bacillomycin- and macrolactin-type antibiotics by bacillus amyloliquefaciens njn-6 for suppressing soilborne plant pathogens. | bacillus amyloliquefaciens strains have been used as biocontrol agents for the suppression of several soilborne plant pathogens. a clearer understanding of the antagonistic mechanisms of action of these bacteria will facilitate their use in the control of plant diseases. antagonistic substances were isolated from the fermentation broth of b. amyloliquefaciens strain njn-6 cultures. these compounds were preconcentrated using an xad-16 column and were purified using reversed-phase high-performance ... | 2012 | 22385216 |
| isolation, identification and characterization of bacillus amyloliquefaciens bz-6, a bacterial isolate for enhancing oil recovery from oily sludge. | over 100 biosurfactant-producing microorganisms were isolated from oily sludge and petroleum-contaminated soil from shengli oil field in north china. sixteen of the bacterial isolates produced biosurfactants and reduced the surface tension of the growth medium from 71 to <30 mn m(-1) after 72 h of growth. these bacteria were used to treat oily sludge and the recovery efficiencies of oil from oily sludge were determined. the oil recovery efficiencies of different isolates ranged from 39% to 88%. ... | 2012 | 22365392 |
| isolation of bacillus amyloliquefaciens strains with antifungal activities from meju. | bacilli with fibrinolytic activities were isolated from traditionally-prepared meju and some of these strains showed strong antifungal activities. one isolate, mj1-4, showed the strongest antifungal activity. mj1-4 and other isolates were identified as b. amyloliquefaciens strains by reca gene sequencing and rapd-pcr results. b. amyloliqufaciens mj1-4 efficiently inhibited an aspergillus spp.-producing aflatoxin b1 (afb1) and a penicillium spp.-producing ochratoxin (ota) in addition to other fun ... | 2012 | 24471064 |
| genome shuffling of bacillus amyloliquefaciens for improving antimicrobial lipopeptide production and an analysis of relative gene expression using fq rt-pcr. | genome shuffling is an efficient approach for the rapid improvement of the yield of secondary metabolites. this study was undertaken to enhance the yield of surfactin produced by bacillus amyloliquefaciens es-2-4 using genome shuffling and to examine changes in srfa expression of the improved phenotype at the transcriptional level. six strains with subtle improvements in lipopeptide yield were obtained from populations generated by ultraviolet irradiation, nitrosoguanidine, and ion beam mutagene ... | 2012 | 22350068 |