| restoration of bioactive lantibiotic suicin from a remnant lan locus of pathogenic streptococcus suis serotype 2. | lantibiotics are ribosomally synthesized, posttranslationally modified antimicrobial peptides. their biosynthesis genes are usually organized in gene clusters, which are mainly found in gram-positive bacteria, including pathogenic streptococci. three highly virulent streptococcus suis serotype 2 strains (98hah33, 05zyh33, and sc84) have been shown to contain an 89k pathogenicity island. here, on these islands, we unveiled and reannotated a putative lantibiotic locus designated sui which contains ... | 2014 | 24271178 |
| development of a lamp assay for rapid detection of different intimin variants of attaching and effacing microbial pathogens. | intimin harboured by pathogenic escherichia coli (e. coli) strains is a key virulence factor involved in host cell adherence and colonization. twenty-seven intimin-encoding e. coli attaching and effacing (eae) gene variants have been reported according to their 3' binding domain sequences. in our study, we developed a specific and sensitive loop-mediated isothermal amplification (lamp) assay to detect all known intimin variants. four primers specific for six regions of eae genes were designed us ... | 2013 | 23893919 |
| identification of in-vivo induced genes of streptococcus suis serotype 2 specially expressed in infected human. | streptococcus suis (s. suis) serotype 2 usually cause infection in swine. recently, two large-scale outbreaks in china with severe streptococcal toxic shock syndrome (stss) and high mortality raised worldwide concern to human s. suis infection. to reveal the molecular pathogenesis of s. suis 2 during human infection, in-vivo induced antigen technology (iviat) was applied to identify the in-vivo induced genes (ivi genes) of s. suis 05zyh33. the ivi genes are specifically expressed or up-regulated ... | 2013 | 23747686 |
| crystallization and preliminary crystallographic analysis of recombinant hyaluronate lyase from streptococcus suis. | hyaluronate lyase is an important surface enzyme of many streptococcal species. the enzyme degrades several biologically important connective tissue components, which facilitates the spreading of the bacteria throughout the host tissues and presumably provides energy and a carbon source for bacterial cells. recombinant hyaluronate lyase was expressed in escherichia coli and was crystallized using the hanging-drop vapour-diffusion method. the crystals belonged to space group p222(1), with unit-ce ... | 2013 | 23722851 |
| [construction and virulence evaluation of the virb1-89k gene knockout mutant of type iv-like secretion system of streptococcus suis serotype 2]. | to construct the virb1-89k gene knockout mutant and its complementary strain of streptococcus suis serotype 2 (ss2) highly virulent strain 05zyh33 and evaluate the role of virb1-89k in the pathogenesis of ss2. | 2013 | 23678574 |
| identification and characterization of a new cell surface protein possessing factor h-binding activity in the swine pathogen and zoonotic agent streptococcus suis. | streptococcus suis is a major swine pathogen and an emerging zoonotic agent. the ability of pathogenic bacteria to bind the complement regulator factor h on their cell surface may allow them to avoid complement attack and phagocytosis. the aim of this study was to characterize a new cell surface protein possessing factor h-binding activity in s. suis serotype 2. the capacity of s. suis to bind the complement regulator factor h on its surface was demonstrated by elisa. using a factor i-cofactor a ... | 2013 | 23618796 |
| a novel fibronectin-binding protein of streptococcus suis serotype 2 contributes to epithelial cell invasion and in vivo dissemination. | streptococcus suis serotype 2 (ss2) is an important pathogen with zoonotic potential. in this study, a novel in vivo induced protein ssa, encoded by the functionally unknown gene ssu05_1311, was identified as a surface anchored fibronectin-binding protein. the recombinant ssa as well as its truncated proteins harboring the n-terminal domain (residues 33-153) could bind to human fibronectin with high-affinity. isogenic knockout of ssa in ss2 led to decrease of bacterial binding to immobilized fib ... | 2013 | 23021642 |
| development of a single-tube loop-mediated isothermal amplification assay for detection of four pathogens of bacterial meningitis. | several loop-mediated isothermal amplification (lamp) assays have been developed to detect common causative pathogens of bacterial meningitis (bm). however, no lamp assay is reported to detect streptococcus agalactiae and streptococcus suis, which are also among common pathogens of bm. moreover, it is laborious and expensive by performing multiple reactions for each sample to detect bacterial pathogen. thus, we aimed to design and develop a single-tube lamp assay capable of detecting multiple ba ... | 2012 | 22946506 |
| future challenges in the elimination of bacterial meningitis. | despite the widespread implementation of several effective vaccines over the past few decades, bacterial meningitis caused by streptococcus pneumoniae, haemophilus influenzae, neisseria meningitidis and group b streptococcus (gbs) still results in unacceptably high levels of human mortality and morbidity. a residual disease burden due to bacterial meningitis is also apparent due to a number of persistent or emerging pathogens, including mycobacterium tuberculosis, escherichia coli, staphylococcu ... | 2012 | 22607903 |
| characterization and determination of holin protein of streptococcus suis bacteriophage smp in heterologous host. | holins are a group of phage-encoded membrane proteins that control access of phage-encoded endolysins to the peptidoglycan, and thereby trigger the lysis process at a precise time point as the 'lysis clock'. smp is an isolated and characterized streptococcus suis lytic phage. the aims of this study were to determine the holin gene, holsmp, in the genome of smp, and characterized the function of holin, holsmp, in phage infection. | 2012 | 22436471 |
| [expression, purification and protective antigen analysis of cell wall protein mrp of streptococcus suis type 2]. | to amplify the mrp gene of streptococcus suis type 2 05zyh33, express it in e.coli bl21 in order to acquire high purity recombinant protein mrp, then evaluate the protective antigen of recombinant protein mrp. | 2012 | 22304764 |
| characterization and functional analysis of atl, a novel gene encoding autolysin in streptococcus suis. | streptococcus suis serotype 2 (s. suis 2) is an important swine and human pathogen responsible for septicemia and meningitis. a novel gene, designated atl and encoding a major autolysin of s. suis 2 virulent strain ha9801, was identified and characterized in this study. the atl protein contains 1,025 amino acids with a predicted molecular mass of 113 kda and has a conserved n-acetylmuramoyl-l-alanine amidase domain. recombinant atl was expressed in escherichia coli, and its bacteriolytic and fib ... | 2012 | 22228730 |
| disposition of ceftiofur and its active metabolites in fallow deer (dama dama) following single-dose intravenous and intramuscular administration. | septicemia and foot infections associated with fusobacterium necrophorum , pasturella multocida, and streptococcus suis in captive fallow deer (dama dama) are reasonably treated with ceftiofur hydrochloride. this study describes the disposition of ceftiofur after single-dose intravenous and intramuscular administration of 3.65±0.1678 mg/kg in six female adult fallow deer using a nonrandomized crossover design and a 7-day washout period. serial blood samples were collected for 12 hr postdrug admi ... | 2015 | 26056876 |
| sialic acid-dependent interactions between influenza viruses and streptococcus suis affect the infection of porcine tracheal cells. | bacterial co-infections are a major complication in influenza-virus-induced disease in both humans and animals. either of the pathogens may induce a host response that affects the infection by the other pathogen. a unique feature in the co-infection by swine influenza viruses (siv) and streptococcus suis serotype 2 is the direct interaction between the two pathogens. it is mediated by the haemagglutinin of siv that recognizes the α2,6-linked sialic acid present in the capsular polysaccharide of ... | 2015 | 26297001 |
| dynamic virus-bacterium interactions in a porcine precision-cut lung slice coinfection model: swine influenza virus paves the way for streptococcus suis infection in a two-step process. | swine influenza virus (siv) and streptococcus suis are common pathogens of the respiratory tract in pigs, with both being associated with pneumonia. the interactions of both pathogens and their contribution to copathogenesis are only poorly understood. in the present study, we established a porcine precision-cut lung slice (pcls) coinfection model and analyzed the effects of a primary siv infection on secondary infection by s. suis at different time points. we found that siv promoted adherence, ... | 2015 | 25916988 |
| transcriptional approach to study porcine tracheal epithelial cells individually or dually infected with swine influenza virus and streptococcus suis. | swine influenza is a highly contagious viral infection in pigs affecting the respiratory tract that can have significant economic impacts. streptococcus suis serotype 2 is one of the most important post-weaning bacterial pathogens in swine causing different infections, including pneumonia. both pathogens are important contributors to the porcine respiratory disease complex. outbreaks of swine influenza virus with a significant level of co-infections due to s. suis have lately been reported. in o ... | 2014 | 24708855 |
| descriptive clinical and epidemiological characteristics of influenza a h1n1 2009 virus infections in pigs in england. | infection of pigs with influenza a h1n1 2009 virus (a(h1n1)pdm09) was first detected in england in november 2009 following global spread of the virus in the human population. this paper describes clinical and epidemiological findings in the first english pig farms in which a(h1n1)pdm09 influenza virus was detected. these farms showed differences in disease presentation, spread and duration of infection. the factors likely to influence these features are described and relate to whether pigs were ... | 2012 | 22865115 |
| multiplex pcr and microarray for detection of swine respiratory pathogens. | porcine respiratory disease complex (prdc) is one of the most important health concerns for pig producers and can involve multiple viral and bacterial pathogens. no simple, single-reaction diagnostic test currently exists for the simultaneous detection of major pathogens commonly associated with prdc. furthermore, the detection of most of the bacterial pathogens implicated in prdc currently requires time-consuming culture-based methods that can take several days to obtain results. in this study, ... | 2015 | 26662640 |
| a newly anti-streptococcus suis bacteriocin producing strain from unweaned piglets fecal matter: isolation, preliminary identification, and optimization of medium composition for enhanced bacteriocin production. | a newly isolated anti-streptococcus suis bacteriocin-producing strain lpl1-5 was obtained from healthy unweaned piglets' fecal matter, and was designated as lactobacillus pentosus lpl1-5 based on morphology, biochemical properties, and 16s rdna sequencing analysis. the medium composition for enhanced bacteriocin production by l. pentosus lpl1-5 was optimized by statistical methodology. yeast extract, k(2)hpo(4)·3h(2)o, and mnso(4)·h(2)o were identified as significant components influencing pento ... | 2012 | 22897764 |
| purification and characterization of suicin 65, a novel class i type b lantibiotic produced by streptococcus suis. | bacteriocins are antimicrobial peptides of bacterial origin that are considered as a promising alternative to the use of conventional antibiotics. recently, our laboratory reported the purification and characterization of two lantibiotics, suicin 90-1330 and suicin 3908, produced by the swine pathogen and zoonotic agent streptococcus suis (serotype 2). in this study, a novel bacteriocin produced by s. suis has been identified and characterized. the producing strain s. suis 65 (serotype 2) was fo ... | 2015 | 26709705 |
| antimicrobial activity of nisin against the swine pathogen streptococcus suis and its synergistic interaction with antibiotics. | streptococcus suis serotype 2 is known to cause severe infections in pigs, including meningitis, endocarditis and pneumonia. furthermore, this bacterium is considered an emerging zoonotic agent. recently, increased antibiotic resistance in s. suis has been reported worldwide. the objective of this study was to evaluate the potential of nisin, a bacteriocin of the lantibiotic class, as an antibacterial agent against the pathogen s. suis serotype 2. in addition, the synergistic activity of nisin i ... | 2013 | 24096107 |
| diadenylate cyclase evaluation of ssdaca (ssu98_1483) in streptococcus suis serotype 2. | cyclic diadenosine monophosphate is a recently identified signaling molecule. it has been shown to play important roles in bacterial pathogenesis. ssu98_1483 (ssdaca), which is an ortholog of listeria monocytogenes daca, is a putative diadenylate cyclase in streptococcus suis serotype 2. in this study, we determined the enzymatic activity of ssdaca in vitro using high-performance liquid chromatography and mass spectrometry. our results showed that ssdaca was a diadenylate cyclase that converts a ... | 2015 | 26125899 |
| multi-etiological nature of tuberculosis-like lesions in condemned pigs at the slaughterhouse. | tuberculosis-like lesions (tbl) in pigs have been associated with microorganisms other than mycobacteria. in this work a histopathological and microbiological evaluation of tbl in pigs is shown. a total of 352 samples belonging to 171 pigs totally condemned at slaughterhouse due to generalized tbl were sampled and selected for analysis. pyogranulomatous (56.2%) and granulomatous lesions (20.2%) were observed in all analysed organs. most of the granulomas observed in both lymph nodes and lungs be ... | 2015 | 26418681 |
| socio-economic, industrial and cultural parameters of pig-borne infections. | the pork-processing industry has been possibly the fastest growing sector of the food industry in recent years. specialization, genetic homogenization of the pig population, high density of the breeding population, reduced human-animal interactions, slaughter at a lower age and increased international trade of live animals and pork are parameters that affect, positively or negatively, the emergence of novel pig-borne pathogens, many of which are pig-specific, and many of which have significant z ... | 2013 | 23738656 |
| surveillance of antimicrobial resistance in clinical isolates of pasteurella multocida and streptococcus suis from ontario swine. | susceptibility results for pasteurella multocida and streptococcus suis isolated from swine clinical samples were obtained from january 1998 to october 2010 from the animal health laboratory at the university of guelph, guelph, ontario, and used to describe variation in antimicrobial resistance (amr) to 4 drugs of importance in the ontario swine industry: ampicillin, tetracycline, tiamulin, and trimethoprim-sulfamethoxazole. four temporal data-analysis options were used: visualization of trends ... | 2014 | 25355992 |
| transcriptional analysis of prrsv-infected porcine dendritic cell response to streptococcus suis infection reveals up-regulation of inflammatory-related genes expression. | the porcine reproductive and respiratory syndrome virus (prrsv) is one of the most important swine pathogens and often serves as an entry door for other viral or bacterial pathogens, of which streptococcus suis is one of the most common. pre-infection with prrsv leads to exacerbated disease caused by s. suis infection. very few studies have assessed the immunological mechanisms underlying this higher susceptibility. since antigen presenting cells play a major role in the initiation of the immune ... | 2016 | 27213692 |
| impact of an experimental prrsv and streptococcus suis coinfection on the pharmacokinetics of ceftiofur hydrochloride after intramuscular injection in pigs. | this study determined the impact of porcine reproductive and respiratory syndrome virus (prrsv) and streptococcus suis coinfection on the pharmacokinetic (pk) profile of ceftiofur hydrochloride in pigs after intramuscular (i.m.) injection. eighteen clinically normal crossbred gilts were assigned by weight into a challenge group (10 pigs) and control group (eight pigs). pigs in both groups received a single i.m. injection of ceftiofur hydrochloride (excenel rtu sterile suspension; zoetis) at a 5 ... | 2015 | 25689130 |
| effects of dietary fibre source on microbiota composition in the large intestine of suckling piglets. | this study aimed to investigate the effects of dietary fibre sources on the gut microbiota in suckling piglets, and to test the hypothesis that a moderate increase of dietary fibre may affect the gut microbiota during the suckling period. suckling piglets were fed different fibre-containing diets or a control diet from postnatal day 7 to 22. digesta samples from cecum, proximal colon and distal colon were used for pig intestinal tract chip analysis. the data showed that the effects of fibre-cont ... | 2016 | 27231242 |
| live attenuated salmonella enterica serovar choleraesuis vaccine vector displaying regulated delayed attenuation and regulated delayed antigen synthesis to confer protection against streptococcus suis in mice. | salmonella enterica serotype choleraesuis (s. choleraesuis) and streptococcus suis (s. suis) are important swine pathogens. development of a safe and effective attenuated s. choleraesuis vaccine vector would open a new window to prevent and control pig diseases. to achieve this goal, the mannose and arabinose regulated delayed attenuated systems (rdas), δpmi and δpcrp::tt arac pbadcrp, were introduced into the wild type s. choleraesuis strain c78-3. we also introduced δrela::arac pbadlaci tt to ... | 2015 | 26238722 |
| streptococcus suis sortase a is ca2+ independent and is inhibited by acteoside, isoquercitrin and baicalin. | sortase a (srta) has long been recognized as an ideal drug target for therapeutic agents against gram-positive pathogens. however, the srta of streptococcus suis (ss-srta), an important zoonotic agent, has not been studied. in this study, the enzymatic properties of ss-srta were investigated, and inhibition of ss-srta by natural products was evaluated. ss-srta was expressed and purified. the purified recombinant ss-srta had maximal activity at ph 6.0-7.5, 45°c, and showed a km of 6.7 μm for the ... | 2017 | 28319184 |
| in vitro and in vivo antibacterial effect of nz2114 against streptococcus suis type 2 infection in mice peritonitis models. | nz2114 is a promising candidate for therapeutic application owing to potent activity to gram-positive bacterium such as streptococcus pneumoniae and staphylococcus aureus. this work is the first report to describe the in vitro and in vivo antibacterial characteristics of nz2114 against streptococcus suis. it exhibited strong antimicrobial activity against s. suis type 2 strains cvcc 606, cvcc 3309, and cvcc 3928 at a low minimal inhibitory concentration (mic) of 0.03-0.06 μm. the nz2114 killed o ... | 2017 | 28220445 |
| expression of a tandemly arrayed plectasin gene from pseudoplectania nigrella in pichia pastoris and its antimicrobial activity. | in recent years, various naturally occurring defence peptides such as plectasin have attracted considerable research interest because they could serve as alternatives to antibiotics. however, the production of plectasin from natural microorganisms is still not commercially feasible because of its low expression levels and weak stability. a tandemly arrayed plectasin gene (1,002 bp) from pseudoplectania nigrella was generated using the isoschizomer construction method, and was inserted into the p ... | 2016 | 26643963 |
| synthesis and antibacterial activity of novel pleuromutilin derivatives. | in this study we describe the design, synthesis, and antibacterial activity of novel pleuromutilin analogs. a series of new compounds containing piperazine and alkylamino or arylamino groups was synthesized. the new compounds were characterized via (1)h-nmr, (13)c-nmr, fourier transform (ft)-ir and ms, and were further evaluated for their in vitro activity against seven gram-positive, and one gram-negative, pathogens. antibacterial data revealed that all compounds exhibited moderate to good anti ... | 2015 | 26133714 |
| identification of the novel lincosamide resistance gene lnu(e) truncated by isenfa5-cfr-isenfa5 insertion in streptococcus suis: de novo synthesis and confirmation of functional activity in staphylococcus aureus. | the novel lincosamide resistance gene lnu(e), truncated by insertion of an isenfa5-cfr-isenfa5 segment, was identified in streptococcus suis. the gene lnu(e) encodes a 173-amino-acid protein with ≤69.4% identity to other lincosamide nucleotidyltransferases. the lnu(e) gene and its promoter region were de novo synthesized, and staphylococcus aureus rn4220 carrying a shuttle vector with the cloned lnu(e) gene showed a 16-fold increase in the lincomycin mic. mass spectrometry experiments demonstrat ... | 2014 | 24366733 |
| novel bacteriophage lysin with broad lytic activity protects against mixed infection by streptococcus pyogenes and methicillin-resistant staphylococcus aureus. | methicillin-resistant staphylococcus aureus (mrsa) and streptococcus pyogenes (group a streptococcus [gras]) cause serious and sometimes fatal human diseases. they are among the many gram-positive pathogens for which resistance to leading antibiotics has emerged. as a result, alternative therapies need to be developed to combat these pathogens. we have identified a novel bacteriophage lysin (plyss2), derived from a streptococcus suis phage, with broad lytic activity against mrsa, vancomycin-inte ... | 2013 | 23571534 |
| identification of a novel collagen type і-binding protein from streptococcus suis serotype 2. | streptococcus suis, a major pathogen of pigs, is an emerging zoonotic agent that causes meningitis and septic shock. cbp40 is a putative virulent gene that has been identified using suppression subtractive hybridization performed on the virulent s. suis serotype 2 strain ha9801 and the avirulent s. suis serotype 2 strain t15. based on predicted protein features showing a shared conserved domain with the collagen-binding protein cna of staphylococcus aureus, cbp40 is likely to function as a direc ... | 2013 | 23465548 |
| screening for antibacterial and antioxidant activities and phytochemical analysis of oroxylum indicum fruit extracts. | oroxylum indicum, which is called pheka in thai, is a traditional thai plant in the bignoniaceae family with various ethnomedical uses such as as an astringent, an anti-inflammatory agent, an anti-bronchitic agent, an anti-helminthic agent and an anti-microbial agent. the young fruits of this plant have also been consumed as vegetables. however, there has been no report concerning its antibacterial activities, especially activities related to clinically isolated pathogenic bacteria and the in vi ... | 2016 | 27070560 |
| antioxidant activity and antibacterial effects on clinical isolated streptococcus suis and staphylococcus intermedius of extracts from several parts of cladogynos orientalis and their phytochemical screenings. | the in vitro antioxidant and antibacterial assays against clinically isolated streptococcus suis and staphylococcus intermedius of the extracts prepared by decoction and ethanolic reflux of different parts of chettaphangki (cladogynos orientalis zipp. ex span), including the leaves, roots, and stems, using 1,1-diphenyl-2-picrylhydrazyl (dpph) scavenging assay and disc diffusion method were conducted. quantitative analysis of total phenolic and total flavonoid contents in the extracts using spect ... | 2015 | 26347795 |
| lysine-tryptophan-crosslinked peptides produced by radical sam enzymes in pathogenic streptococci. | macrocycles represent a common structural framework in many naturally occurring peptides. several strategies exist for macrocyclization, and the enzymes that incorporate them are of great interest, as they enhance our repertoire for creating complex molecules. we recently discovered a new peptide cyclization reaction involving a crosslink between the side chains of lysine and tryptophan that is installed by a radical sam enzyme. herein, we characterize relatives of this metalloenzyme from the pa ... | 2017 | 28191919 |
| evolution and diversity of the antimicrobial resistance associated mobilome in streptococcus suis: a probable mobile genetic elements reservoir for other streptococci. | streptococcus suis is a previously neglected, newly emerging multidrug-resistant zoonotic pathogen. mobile genetic elements (mges) play a key role in intra- and interspecies horizontal transfer of antimicrobial resistance (amr) determinants. although, previous studies showed the presence of several mges, a comprehensive analysis of amr-associated mobilome as well as their interaction and evolution has not been performed. in this study, we presented the amr-associated mobilome and their insertion ... | 2016 | 27774436 |
| comparative genomic analysis of the icesa2603 family ices and spread of erm(b)- and tet(o)-carrying transferable 89k-subtype ices in swine and bovine isolates in china. | integrative and conjugative elements (ices) of the icesa2603 family have been isolated from several species of streptococcus spp.; however, the comparative genomic and evolutionary analyses of these particular ices are currently only at their initial stages. by investigating 13 ices of the icesa2603 family and two icesa2603 family-like ices derived from diverse hosts and locations, we have determined that ices comprised a backbone of 30 identical syntenic core genes and accessory genes that were ... | 2016 | 26870017 |
| genomic comparison between pathogenic streptococcus agalactiae isolated from nile tilapia in thailand and fish-derived st7 strains. | streptococcus agalactiae, or group b streptococcus (gbs), is a highly virulent pathogen in aquatic animals, causing huge mortalities worldwide. in thailand, the serotype ia, β-hemolytic gbs, belonging to sequence type (st) 7 of clonal complex (cc) 7, was found to be the major cause of streptococcosis outbreaks in fish farms. in this study, we performed an in silico genomic comparison, aiming to investigate the phylogenetic relationship between the pathogenic fish strains of thai st7 and other st ... | 2015 | 26455417 |
| recombination between streptococcus suis icessu32457 and streptococcus agalactiae icesa2603 yields a hybrid ice transferable to streptococcus pyogenes. | integrative conjugative elements (ices) are mobile genetic elements that reside in the chromosome but retain the ability to undergo excision and to transfer by conjugation. genes involved in drug resistance, virulence, or niche adaptation are often found among backbone genes as cargo dna. we recently characterized in streptococcus suis an ice (icessu32457) carrying resistance genes [tet(o/w/32/o), tet(40), erm(b), apha, and aade] in the 15k unstable genetic element, which is flanked by two ∼1.3k ... | 2015 | 25935120 |
| transduction of the streptococcus pyogenes bacteriophage φm46.1, carrying resistance genes mef(a) and tet(o), to other streptococcus species. | φm46.1 - streptococcus pyogenes bacteriophage carrying mef(a) and tet(o), respectively, encoding resistance to macrolides (m phenotype) and tetracycline - is widespread in s. pyogenes but has not been reported outside this species. φm46.1 is transferable in vitro among s. pyogenes isolates, but no information is available about its transferability to other streptococcus species. we thus investigated φm46.1 for its ability to be transduced in vitro to recipients of different streptococcus species ... | 2014 | 25620959 |
| common regulators of virulence in streptococci. | streptococcal species are a diverse group of bacteria which can be found in animals and humans. their interactions with host organisms can vary from commensal to pathogenic. many of the pathogenic species are causative agents of severe, invasive infections in their hosts, accounting for a high burden of morbidity and mortality, associated with high economic costs in industry and health care. among them, streptococcus pyogenes, streptococcus agalactiae, streptococcus pneumoniae, and streptococcus ... | 2013 | 23242855 |
| interspecies mobilization of an ermt-carrying plasmid of streptococcus dysgalactiae subsp. equisimilis by a coresident ice of the icesa2603 family. | the recently documented presence of almost identical, small, non-self-transmissible, erm(t)-carrying plasmids in clonally unrelated erythromycin-resistant isolates of streptococcus pyogenes and streptococcus agalactiae suggests that these plasmids somehow circulate in the streptococcal population. the objective of this study was to characterize the erm(t)-carrying genetic element in a clinical isolate of streptococcus dysgalactiae subsp. equisimilis (sde5580) and to provide a possible explanatio ... | 2013 | 22949621 |
| characterization of a streptococcus suis tet(o/w/32/o)-carrying element transferable to major streptococcal pathogens. | mosaic tetracycline resistance determinants are a recently discovered class of hybrids of ribosomal protection tet genes. they may show different patterns of mosaicism, but their final size has remained unaltered. initially thought to be confined to a small group of anaerobic bacteria, mosaic tet genes were then found to be widespread. in the genus streptococcus, a mosaic tet gene [tet(o/w/32/o)] was first discovered in streptococcus suis, an emerging drug-resistant pig and human pathogen. in th ... | 2012 | 22710115 |
| molecular and antimicrobial susceptibility profiling of atypical streptococcus species from porcine clinical specimens. | the streptococcus species present broad phenotypic variation, making identification difficult using only traditional microbiological methods. even though streptococcus suis is the most important species for the worldwide swine industry, other streptococcus species appear to be able to cause disease in swine and could represent a higher underestimated risk for porcine health. the aim of this study was to identify streptococcus-like isolates by maldi-tof ms and 16s rrna sequencing and further mole ... | 2016 | 27491454 |
| epidemiology and pathogenicity of zoonotic streptococci. | zoonotic infections caused by streptococcus spp. have been neglected in spite of the fact that frequency and severity of outbreaks increased dramatically in recent years. this may be due to non-identification since respective species are often not considered in human medical diagnostic procedures. on the other hand, an expanding human population concomitant with an increasing demand for food and the increased number of companion animals favour conditions for host species adaptation of animal str ... | 2013 | 23192319 |
| survival of streptococcus suis, streptococcus dysgalactiae and trueperella pyogenes in dry-cured iberian pork shoulders and loins. | dry-cured hams, shoulders and loins of iberian pigs are highly appreciated in national and international markets. salting, additive addition and dehydration are the main strategies to produce these ready-to-eat products. although the dry curing process is known to reduce the load of well-known food borne pathogens, studies evaluating the viability of other microorganisms in contaminated pork have not been performed. in this work, the efficacy of the dry curing process to eliminate three swine pa ... | 2017 | 27697171 |
| a novel suicide shuttle plasmid for streptococcus suis serotype 2 and streptococcus equi ssp. zooepidemicus gene mutation. | the mariner-based himar1 system has been utilized for creating mutant libraries of many gram-positive bacteria. streptococcus suis serotype 2 (ss2) and streptococcus equi ssp. zooepidemicus (sez) are primary pathogens of swine that threaten the swine industry in china. to provide a forward-genetics technology for finding virulent phenotype-related genes in these two pathogens, we constructed a novel temperature-sensitive suicide shuttle plasmid, pmar4s, which contains the himar1 system transposo ... | 2016 | 27256117 |
| prophage lysin ply30 protects mice from streptococcus suis and streptococcus equi subsp. zooepidemicus infections. | streptococcus suis and streptococcus equi subsp. zooepidemicus are capable of infecting humans and various animals, causing significant problems for the worldwide swine industry. as antibiotic resistance has increased, lysosomal enzymes encoded by phages have shown potential for use against pathogenic bacteria. in this study, a novel bacteriophage lysin, ply30, encoded by the s. suis prophage phi30c, was recombinantly expressed and purified. ply30 showed high bacteriolysis activity on s. suis an ... | 2015 | 26253669 |
| temporal regulation of the transformasome and competence development in streptococcus suis. | in s. suis the comx-inducing peptide (xip) pheromone regulates comr-dependent transcriptional activation of comx (or sigx) the regulator of the late competence regulon. the aims of this study were to identify the comr-regulated genes and in s. suis using genome-wide transcriptomics and identify their function based on orthology and the construction of specific knockout mutants. the comx regulon we identified, includes all homologs of the "transformasome" a type 4-like pilus dna binding and trans ... | 2016 | 28066332 |
| streptococcusmarmotae sp. nov., isolated from the respiratory tract of marmota himalayana. | five strains of a gram-stain-positive, catalase-negative, α-haemolytic, coccus-shaped chain-forming organism were isolated separately from the lower respiratory tracts of five animals of marmota himalayana in the endemic area of plague, the qinghai-tibet plateau, china. based on their morphological characteristics, biochemical features and molecular phylogenetic studies, the strains were placed as representing a new member of the genus streptococcus. comparative 16s rrna gene sequence studies in ... | 2016 | 27473166 |
| heat incubation inactivates streptococcal exotoxins and recombinant cholesterol-dependent cytolysins: suilysin, pneumolysin and streptolysin o. | streptococcus species release cholesterol-dependent cytolysins (cdcs), which are a main toxin, and their heat susceptibility is poorly understood. the aim of this study was to clarify the heat susceptibility of streptococcal exotoxins and cdcs. streptococcal exotoxins were treated with heat incubation at 60 °c for 10 or 30 min. the streptococcus suis exotoxin of serotypes 1 and 2 exhibited more than 50 % haemolytic activity, and all streptococcus pneumoniae exotoxins exhibited more than 60 % hae ... | 2014 | 24974216 |
| insight into the evolution of the histidine triad protein (htp) family in streptococcus. | the histidine triad proteins (htps), also known as pht proteins in streptococcus pneumoniae, constitute a family of surface-exposed proteins that exist in many pathogenic streptococcal species. although many studies have revealed the importance of htps in streptococcal physiology and pathogenicity, little is known about their origin and evolution. in this study, after identifying all htp homologs from 105 streptococcal genomes representing 38 different species/subspecies, we analyzed their domai ... | 2013 | 23527301 |
| an octamer of enolase from streptococcus suis. | enolase is a conserved cytoplasmic metalloenzyme existing universally in both eukaryotic and prokaryotic cells. the enzyme can also locate on the cell surface and bind to plasminogen, via which contributing to the mucosal surface localization of the bacterial pathogens and assisting the invasion into the host cells. the functions of the eukaryotic enzymes on the cell surface expression (including t cells, b cells, neutrophils, monocytoes, neuronal cells and epithelial cells) are not known. strep ... | 2012 | 23055041 |
| [cloning and analysis of the conserved regions in the capsular polysaccharides synthesis locus of streptococcus suis]. | to learn about the function and gene phylogenetic relationship of the conserved regions in capsular polysaccharides synthesis (cps) locus, | 2012 | 22803342 |
| suilysin-induced platelet-neutrophil complexes formation is triggered by pore formation-dependent calcium influx. | platelet activation and platelet-neutrophil interactions have been found to be involved in inflammation, organ failure and soft-tissue necrosis in bacterial infections. streptococcus suis, an emerging human pathogen, can cause streptococcal toxic-shock syndrome (stss) similarly to streptococcus pyogenes. currently, s. suis-platelet interactions are poorly understood. here, we found that suilysin (sly), the s. suis cholesterol-dependent cytolysin (cdc), was the sole stimulus of s. suis that induc ... | 2016 | 27830834 |
| the two-component system ihk/irr contributes to the virulence of streptococcus suis serotype 2 strain 05zyh33 through alteration of the bacterial cell metabolism. | streptococcus suis serotype 2 (s. suis 2) is an important zoonotic pathogen. it causes heavy economic losses in the pig-farming industry and can be associated with severe infections in humans, e.g. streptococcal toxic shock syndrome. understanding its pathogenesis is critical for prevention and control of diseases caused by s. suis 2. in this study, we show that deletion of a two-component system (tcs), 05ssu1660/1661 (orthologues of the ihk/irr tcs of streptococcus pyogenes), in s. suis 2 strai ... | 2012 | 22504441 |
| a locus encoding variable defence systems against invading dna identified in streptococcus suis. | streptococcus suis, an important zoonotic pathogen, is known to have an open pan-genome and to develop a competent state. in s. suis, limited genetic lineages are suggested to be associated with zoonosis. however, little is known about the evolution of diversified lineages and their respective phenotypic or ecological characteristics. in this study, we performed comparative genome analyses of s. suis, with a focus on the competence genes, mobile genetic elements (mges), and genetic elements rela ... | 2017 | 28379509 |
| streptococcus suis serotype 2 biofilms inhibit the formation of neutrophil extracellular traps. | invasive infections caused by streptococcus suis serotype 2 (ss2) has emerged as a clinical problem in recent years. neutrophil extracellular traps (nets) are an important mechanism for the trapping and killing of pathogens that are resistant to phagocytosis. biofilm formation can protect bacteria from being killed by phagocytes. until now, there have only been a few studies that focused on the interactions between bacterial biofilms and nets. ss2 in both a biofilm state and a planktonic cell st ... | 2017 | 28373968 |
| morin attenuates streptococcus suis pathogenicity in mice by neutralizing suilysin activity. | streptococcus suis, a gram-positive pathogen, is widely recognized as an important agent of swine infection, and it is also known to cause a variety of zoonoses, such as meningitis, polyarthritis and pneumonia. suilysin (sly), an extracellular pore-forming toxin that belongs to the cholesterol-dependent cytolysin family, is an essential virulence factor of s. suis capsular type 2 (ss2). here, we found that morin hydrate (morin), a natural flavonoid that lacks anti-ss2 activity, inhibits the hemo ... | 2017 | 28373868 |
| the non-conserved region of mrp is involved in the virulence of streptococcus suis serotype 2. | muramidase-released protein (mrp) of streptococcus suis serotype 2 (ss2) is an important epidemic virulence marker with an unclear role in bacterial infection. to investigate the biological functions of mrp, three mutants named δmrp, δmrp domain 1 (δmrp-d1), and δmrp domain 2 (δmrp-d2) were constructed to assess the phenotypic changes between the parental strain and the mutant strains. the results indicated that mrp domain 1 (mrp-d1, the non-conserved region of mrp from a virulent strain, a.a. 2 ... | 2017 | 28362221 |
| streptococcus suis serotype 2 strains isolated in argentina (south america) are different from those recovered in north america and present a higher risk for humans. | streptococcus suis serotype 2 is an important swine pathogen and emerging zoonotic agent causing meningitis and septicemia/septic shock. strains are usually virulent (eurasia) or of intermediate/low virulence (north america). very few data regarding human and swine isolates from south america are available. | 2016 | 28348788 |
| porcine dendritic cells as an in vitro model to assess the immunological behaviour of streptococcus suis subunit vaccine formulations and the polarizing effect of adjuvants. | an in vitro porcine bone marrow-derived dendritic cell (dc) culture was developed as a model for evaluating immune polarization induced by adjuvants when administered with immunogens that may become vaccine candidates if appropriately formulated. the swine pathogen streptococcus suis was chosen as a prototype to evaluate proposed s. suis vaccine candidates in combination with the adjuvants poly i:c, quil a ®, alhydrogel ®, titermax gold ® and stimune ®. the toll-like receptor ligand poly i:c and ... | 2017 | 28327531 |
| the eukaryote-like serine/threonine kinase stk regulates the growth and metabolism of zoonotic streptococcus suis. | like eukaryotes, bacteria express one or more serine/threonine kinases (stks) that initiate diverse signaling networks. the stk from streptococcus suis is encoded by a single-copy stk gene, which is crucial in stress response and virulence. to further understand the regulatory mechanism of stk in s. suis, a stk deletion strain (δstk) and its complementary strain (cδstk) were constructed to systematically decode stk characteristics by applying whole transcriptome rna sequencing (rna-seq) and phos ... | 2017 | 28326294 |
| better understanding of homologous recombination through a 12-week laboratory course for undergraduates majoring in biotechnology. | homologous recombination, a central concept in biology, is defined as the exchange of dna strands between two similar or identical nucleotide sequences. unfortunately, undergraduate students majoring in biotechnology often experience difficulties in understanding the molecular basis of homologous recombination. in this study, we developed and implemented a 12-week laboratory course for biotechnology undergraduates in which gene targeting in streptococcus suis was used to facilitate their underst ... | 2017 | 28314074 |
| genome-wide analysis of a avirulent and reveal the strain induces pro-tective immunity against challenge with virulent streptococcus suis serotype 2. | it was previously reported in china that two recent large-scale outbreaks of streptococcus suis serotype 2 (s. suis 2) infections in human were caused by two highly virulent s. suis 2 strains, from which a novel genomic island (gei), associated with disease onset and progression and designated 89 k, was identified. here, an avirulent strain, 05has68, was isolated from a clinically healthy pig. | 2017 | 28292261 |
| critical streptococcus suis virulence factors: are they all really critical? | streptococcus suis is an important swine pathogen that can be transmitted to humans by contact with diseased animals or contaminated raw pork products. this pathogen possesses a coat of capsular polysaccharide (cps) that confers protection against the immune system. yet, the cps is not the only virulence factor enabling this bacterium to successfully colonize, invade, and disseminate in its host leading to severe systemic diseases such as meningitis and toxic shock-like syndrome. indeed, recent ... | 2017 | 28274524 |
| case report: two human streptococcus suis infections in borneo, sabah, malaysia. | streptococcus suis (s.suis) is increasingly being recognised as a potentially preventable emerging zoonotic infection in humans with a global distribution. it is a major cause of meningitis especially among those in contact with pigs and has also been associated with a toxic shock syndrome. | 2017 | 28257622 |
| capsular serotypes, virulence-associated genes and antimicrobial susceptibility of streptococcus suis isolates from pigs in korea. | streptococcus suis is an important pig pathogen with potential for human transmission. the serotype distributions and phenotypic characteristics vary over time and among regions; however, little is known about the characteristics of s. suis isolates in korea. in this study, 240 s. suis isolates collected from pigs in korea in 2009-2010 were serotyped by coagglutination tests, subsequently screened for three virulence-associated genes (mrp, epf and sly) and tested for antimicrobial susceptibility ... | 2017 | 28250312 |
| genotyping and investigating capsular polysaccharide synthesis gene loci of non-serotypeable streptococcus suis isolated from diseased pigs in canada. | streptococcus suis (s. suis) is an important swine pathogen and an emerging zoonotic agent. most clinical s. suis strains express capsular polysaccharides (cps), which can be typed by antisera using the coagglutination test. in this study, 79 s. suis strains recovered from diseased pigs in canada and which could not be typed using antisera were further characterized by capsular gene typing and sequencing. four patterns of cps locus were observed: (1) fifteen strains were grouped into previously ... | 2017 | 28219415 |
| transcriptomic analysis reveals selective metabolic adaptation of streptococcus suis to porcine blood and cerebrospinal fluid. | streptococcus suis is a zoonotic pathogen that can cause severe pathologies such as septicemia and meningitis in its natural porcine host as well as in humans. establishment of disease requires not only virulence of the infecting strain but also an appropriate metabolic activity of the pathogen in its host environment. however, it is yet largely unknown how the streptococcal metabolism adapts to the different host niches encountered during infection. our previous isotopologue profiling studies o ... | 2017 | 28212285 |
| syringa oblata lindl. aqueous extract is a potential biofilm inhibitor in s. suis. | streptococcus suis (s. suis) is a zoonotic pathogen that causes severe disease symptoms in pigs and humans. syringa oblata lindl. distributed in the middle latitudes of eurasia and north america were proved as the most development potential of chinese medicine. in this study, biofilm formation by s. suis decreased after growth with 1/2 mic, 1/4 mic, or 1/8 mic of syringa oblata lindl. aqueous extract and rutin. scanning electron microscopy analysis revealed the potential effect of syringa oblata ... | 2017 | 28194111 |
| isolation and partial characterization of streptococcus suis from clinical cases in cattle. | sixteen isolates of gram-positive, coccoid bacteria were obtained from clinical cases of diverse conditions in cattle and identified as streptococcus suis using 16s ribosomal dna gene sequencing and other bacterial identification methods. none of the isolates could be assigned to any of the known s. suis capsular types. virulence-associated gene profiling that targeted muramidase-released protein, extracellular protein factor, suilysin, 89-kb pathogenicity island, and arginine deiminase ( arca) ... | 2017 | 28166707 |
| factor h specifically capture novel factor h-binding proteins of streptococcus suis and contribute to the virulence of the bacteria. | factor h (fh), a regulatory protein of the complement system, can bind specifically to factor h-binding proteins (fhbps) of streptococcus suis serotype 2 (ss2), which contribute to evasion of host innate immune defenses. in the present study, we aimed to identify novel fhbps and characterize the biological functions of fh in ss2 pathogenesis. here, a method that combined proteomics and far-western blotting was developed to identify the surface fhbps of ss2. with this method, fourteen potential n ... | 2017 | 28164787 |
| transcutaneous immunization of streptococcus suis bacterin using dissolving microneedles. | vaccine delivery using microneedle (mn) patches is an easy, safe and painless alternative to traditional needle injections. in this study, we examined whether mn patches can enhance the efficacy of a streptococcus suis serotype 2 (s. suis 2) vaccine in a mouse model. results showed that mns can reach 200-250μm into the skin, a depth beneficial for targeted delivery of antigens to antigen-presenting cells in the epidermis and dermis. vaccination with prime-boost of mn induced higher levels of igg ... | 2017 | 28131383 |
| streptococcus suis small rna rss04 contributes to the induction of meningitis by regulating capsule synthesis and by inducing biofilm formation in a mouse infection model. | streptococcus suis (ss) is an important pathogen for pigs, and it is also considered as a zoonotic agent for humans. meningitis is one of the most common features of the infection caused by ss, but little is known about the mechanisms of ss meningitis. recent studies have revealed that small rnas (srnas) have emerged as key regulators of the virulence in several bacteria. in the previous study, we reported that ss srna rss04 was up-regulated in pig cerebrospinal fluid and contributes to ss virul ... | 2017 | 28110777 |
| draft genome sequence of hypervirulent and vaccine candidate streptococcus suis strain sc19. | streptococcus suis, a zoonotic bacterium found primarily in pigs, has been recognized recently as an emerging pathogen of humans. herein, we describe the genome of streptococcus suis strain sc19, a hypervirulent and vaccine candidate strain isolated from a pig amid the 2005 outbreak in china. | 2017 | 28104658 |
| the involvement of msmk in pathogenesis of the streptococcus suis serotype 2. | streptococcus suis serotype 2 (ss2) is an important swine and human pathogen that causes global economic and public health problems. virulent s. suis strains successfully maintain high bacterial concentrations in host blood and rapidly adapt to challenging environments within hosts. successful survival in hosts is a major factor influencing the pathogenesis of ss2. we have previously identified that ss2 colonization in mouse brain is possibly affected by the atpase, msmk of carbohydrate atp-bind ... | 2017 | 28102028 |
| serotypes of streptococcus suis isolated from healthy pigs in phayao province, thailand. | streptococcus suis (s. suis) is an important swine and human pathogen. there are 33 serotypes that have been described. zoonotic cases are very common the northern part of thailand, especially in phayao province. however, the prevalence of s. suis and, more particularly the different serotypes, in pigs in this region is poorly known and needed to be addressed. | 2017 | 28100261 |
| the 1910hk/rr two-component system is essential for the virulence of streptococcus suis serotype 2. | streptococcus suis serotype 2 is a major zoonotic pathogen, and the two-component system plays an important role in bacterial pathogenesis. the present study targeted the 1910hk/rr two-component system of s. suis 2. a 1910hk/rr deletion mutant (δ1910hk/rr) and the corresponding complementation strain (cδ1910hk/rr) were constructed in s. suis 2 strain 05zyh33. 1910hk/rr deletion had no effect on s. suis 2 growth, but significantly inhibited the adherence and invasion of s. suis 2 to hep-2 cells. ... | 2017 | 28093234 |
| pathogenic streptococcus strains employ novel escape strategy to inhibit bacteriostatic effect mediated by mammalian peptidoglycan recognition protein. | pathogenic streptococcal species are responsible for some of the most lethal and prevalent animal and human infections. previous reports have identified a candidate pathogenicity island (pai) in two highly virulent clinical isolates of streptococcus suis type 2, a causative agent of high-mortality streptococcal toxic shock syndrome. this pai contains a type-ivc secretion system c subgroup (type-ivc secretion system) that is involved in the secretion of unknown pathogenic effectors that are respo ... | 2017 | 28092693 |
| deletion of ssna attenuates the pathogenicity of streptococcus suis and confers protection against serovar 2 strain challenge. | streptococcus suis serotype 2 (ss2) is a major porcine and human pathogen which causes arthritis, meningitis, and septicemia. streptococcus suis nuclease a (ssna) is a recently discovered deoxyribonuclease (dnase), which has been demonstrated to contribute to escape killing in neutrophil extracellular traps (nets). to further determine the effects of ssna on virulence, the ssna deletion mutant (δssna) and its complemented strain (c-δssna) were constructed. the ability of δssna mutant to interact ... | 2017 | 28081204 |
| distribution of suicin gene clusters in streptococcus suis serotype 2 belonging to sequence types 25 and 28. | recently, we reported the purification and characterization of three distinct lantibiotics (named suicin 90-1330, suicin 3908, and suicin 65) produced by streptococcus suis. in this study, we investigated the distribution of the three suicin lantibiotic gene clusters among serotype 2 s. suis strains belonging to sequence type (st) 25 and st28, the two dominant sts identified in north america. the genomes of 102 strains were interrogated for the presence of suicin gene clusters encoding suicins 9 ... | 2016 | 28078298 |
| immunization with streptococcus suis bacterin plus recombinant sao protein in sows conveys passive immunity to their piglets. | streptococcus suis (s. suis) causes arthritis, meningitis, septicemia, and sudden death in pigs and is also an zoonotic agent for humans. the present study demonstrated that immunization with recombinant sao-l (surface antigen one-l, rsao-l) protein from a strain of s. suis serotype 2 in pigs was able to increase cross-serotype protection against s. suis serotype 1 and 2 challenge. since weaning piglets are more susceptible to s. suis infections due to the stresses associated with weaning, prepa ... | 2017 | 28061775 |
| development of an indirect dot-ppa-elisa using glutamate dehydrogenase as a diagnostic antigen for the rapid and specific detection of streptococcus suis and its application to clinical specimens. | streptococcus suis is an important zoonotic pathogen causing infections in pigs and humans. bacterial surface-related proteins are often explored as potential vaccine candidates and diagnostic antigens. in the present study, glutamate dehydrogenase, a highly conserved immunogenic extracellular protein, was used to establish a dot horseradish peroxidase enzyme-linked staphylococcal protein a immunosorbent assay (dot-ppa-elisa) for diagnosis of s. suis infection. the antigen-antibody reaction was ... | 2017 | 28058577 |
| a serine/threonine phosphatase 1 of streptococcus suis type 2 is an important virulence factor. | streptococcus suis is regarded as one of the major pathogens of pigs while s. suis type 2 (ss2) is considered to be a zoonotic bacterium for its ability to cause meningitis and streptococcal toxic shock-like syndrome in humans. many bacterial species contain genes encoding serine/threonine protein phosphatases (stp) responsible for dephosphorylation of their substrates in a single reaction step. this study was aimed to investigate the role of stp1 in pathogenesis of ss2. an isogenic stp1 mutant ... | 2017 | 28057904 |
| the phage lysin plyss2 decolonizes streptococcus suis from murine intranasal mucosa. | streptococcus suis infects pigs worldwide and may be zoonotically transmitted to humans with a mortality rate of up to 20%. s. suis has been shown to develop in vitro resistance to the two leading drugs of choice, penicillin and gentamicin. because of this, we have pursued an alternative therapy to treat these pathogens using bacteriophage lysins. the bacteriophage lysin plyss2 is derived from an s. suis phage and displays potent lytic activity against most strains of that species including sero ... | 2017 | 28046082 |
| detection of multiple parallel transmission outbreak of streptococcus suis human infection by use of genome epidemiology, china, 2005. | streptococcus suis sequence type 7 emerged and caused 2 of the largest human infection outbreaks in china in 1998 and 2005. to determine the major risk factors and source of the infections, we analyzed whole genomes of 95 outbreak-associated isolates, identified 160 single nucleotide polymorphisms, and classified them into 6 clades. molecular clock analysis revealed that clade 1 (responsible for the 1998 outbreak) emerged in october 1997. clades 2-6 (responsible for the 2005 outbreak) emerged se ... | 2017 | 27997331 |
| distribution of type i restriction-modification systems in streptococcus suis: an outlook. | streptococcus suis is a porcine commensal and pathogen with zoonotic potential. we recently identified a novel type i restriction-modification (r-m) system in a zoonotic s. suis clone which has emerged in the netherlands. here, we describe the dna inversions in the specificity subunit of this system in s. suis serotype 2, clonal complex 20 and explain the absence of domain movement by the absence of repeats. in addition, we identified a core type i r-m system present in 95% of the isolates and f ... | 2016 | 27869755 |
| mac protein is not an essential virulence factor for the virulent reference strain streptococcus suis p1/7. | streptococcus suis is a major pathogen of pigs and also an important zoonotic agent for humans. a s. suis protein containing mac-1 domain (designated mac) is a protective antigen, exclusively cleaves porcine igm, and contributes to complement evasion with the presence of high titers of specific porcine anti-s. suis igm, but its role in s. suis virulence has not been investigated in natural healthy host without specific igm. in this study, a mac deletion mutant was constructed by homologous recom ... | 2017 | 27847975 |
| structural and functional analysis of an anchorless fibronectin-binding protein fbps from gram-positive bacterium streptococcus suis. | the anchorless fibronectin-binding proteins (fnbps) are a group of important virulence factors for which the structures are not available and the functions are not well defined. in this study we performed comprehensive studies on a prototypic member of this group: the fibronectin-/fibrinogen-binding protein from streptococcus suis (fbps). the structures of the n- and c-terminal halves (fbps-n and fbps-c), which together cover the full-length protein in sequence, were solved at a resolution of 2. ... | 2016 | 27834729 |
| human meningitis due to streptococcus suis in lomé, togo: a case report. | streptococcus suis is a zoonotic pathogen which represents the leading cause of meningitis in southeast asia and an emerging pathogen in the western world, the main risk factor for infection being contact with pigs. in africa, the prevalence of s. suis infections in swine and humans is largely unrecognized, with only one recent report of a limited case series. | 2016 | 27821092 |
| effects of suilysin on streptococcus suis-induced platelet aggregation. | blood platelets play important roles during pathological thrombocytopenia in streptococcal toxic shock syndrome (stss). streptococcus suis (s. suis) an emerging human pathogen, can cause stss similarly to s. pyogenes. however, s. suis interactions with platelets are poorly understood. here, we found that suilysin (sly), different from other bacterial cholesterol-dependent cytolysins (cdcs), was the sole stimulus that induced platelet aggregation. furthermore, the inside-out activation of gpiib/i ... | 2016 | 27800304 |
| a decennium of etiology and antimicrobial susceptibility patterns in patients with infective endocarditis at a university hospital, thailand. | infective endocarditis is an infection with a high mortality rate. antimicrobial therapy is important in treatment but data on antimicrobial susceptibilities are limited. this retrospective study analyzed the data of causative microorganisms and antimicrobial susceptibility patterns in patients with infective endocarditis who were aged 18 years or older and received inpatient care between 2006 and 2015 at king chulalongkorn memorial hospital. a total of 213 patients fulfilled the inclusion crite ... | 2016 | 27795476 |
| a fatal case of streptococcal toxic shock syndrome caused by streptococcus suis carrying tet (40) and tet (o/w/32/o), italy. | we report the first human fatal case of streptococcal toxic shock syndrome (stss) caused by streptococcus suis serotype 2 carrying the tetracycline efflux tet (40) gene and the tetracycline ribosomal protection tet (o/w/32/o) gene. the patient was splenectomized. the case was characterized by multi-organ dysfunction and disseminated intravascular coagulopathy, in accordance with the clinical parameters of stss. more investigations are needed to improve the epidemiology and the pathogenesis of s. ... | 2016 | 27553071 |
| first case in spain of septic shock by streptococcus suis possible food-borne. | | 2016 | 27021908 |
| predominance of streptococcus suis st1 and st7 in human cases in china, and detection of a novel sequence type, st658. | | 2016 | 27689249 |