| [the localization of alpha-fetoprotein in the mouse fetus by immunofluorescence and immunodiffusion tests]. | | 1979 | 95700 |
| evidence for transmission of lymphocyte responses to tuberculin by breast-feeding. | the possibility that cell-mediated immunity could be acquired by breast-feeding was evaluated in a prospective study of 26 tuberculin positive and 9 negative puerperal mothers and their infants. 13 infants of the positive and all the infants of negative mothers were breast-fed. tuberculin-reactive t cells were found in colostrum and early milk of most positive but in none of the negative nursing mothers. a significant number (8/13) of infants born to positive mothers had tuberculin-reactive peri ... | 1977 | 95733 |
| "micrococci" and urinary infection. | | 1977 | 95777 |
| binding of mouse peritoneal macrophages to tumour cells by a "lectin-like" macrophage receptor. | a binding interaction is described between mouse peritoneal macrophages, tumour cells and proliferating mouse embryo fibroblasts. the interaction appears to be between a "lectin-like" receptor in the macrophage plasma membrane and carbohydrates in the plasma membrane of the target cells. | 1979 | 95806 |
| [the proventriculus of the xenopsylla cheopis flea studied by scanning electron microscopy]. | the proventriculus of the flea x. cheopis was studied by means of scanning electron microscopy. the external surface of the proventriculus is a rigid structure with an alveolate surface ("wasp nest") formed by muscular cords. the internal surface of the proventriculus is represented by numerous acanthae which fall close together. they have a shape of bent dens with longitudinal sharply angular edges and concave spaces between them. some edges are crenate. | 1979 | 95815 |
| [biosynthesis of penicillin upon periodic culturing of penicillium chrysogenum with an exponentially increasing glucose supply]. | penicillium chrysogenum was cultivated under controlled conditions and at constant specific growth rates regulated by glucose supply. the rate of penicillin synthesis (units/mg dry weight.hr) and pattern of time changes of the synthesizing capacity of the culture depended on the specific growth rate (hr-1). the arrest of penicillin synthesis was induced by the inherent changes of the culture rather than by external factors, e.g. use of substrates, changed limitation, accumulation of inhibitory m ... | 1979 | 95828 |
| [external brain acetylcholinesterase in the ontogeny of precocial and altricial rodents]. | the share of external acetylcholinesterase (ache) has been determined in different brain structures of maturely and immaturely born rodents. it was found that in maturely born animals (guinea pigs) the share of external ache in all the studied brain structures is the same in newborn and adult individuals. in immaturely born animals (albino rats) the share of external ache to the moment of birth is significantly higher as that in adult organisms. during the development of the brain, the share of ... | 1979 | 95839 |
| [the characteristics of the potential of the dorsal surface of the spinal cord in week-old rat pups]. | in 7-day albino rats, the negative component of the potential of the dorsal surface of the spinal cord during stimulation of the nervous trunk exhibits long latent period, low amplitude and significant duration. slow positive potential has a low amplitude, whereas its duration is approximately the same as that in adult rats. the presence of a slow positive wave in 7-day rats indicates a possibility of presynaptic inhibition already at the beginning of postnatal life of these animals. | 1979 | 95858 |
| [preservation of hematopoietic stem cells by slow freezing and elimination of the heat of fusion]. | previous experiments, in dogs with fresh bone marrow or bone marrow frozen with a modified freezing system have demonstrated a 100% recovery of frozen stem cells, stored for periods up to 5 months. five patients, three with drug resistant acute leukemia and two with metastic carcinomas, have been treated with a high dose combination chemotherapy regimen (tacc) followed by reinfusion of marrow cryopreserved with the same modified freezing system. following the reinfusion of marrow, autologous en ... | 1978 | 95897 |
| isolation and properties of a succinylated subunit of human thyroglobulin. | a subunit of succinylated (40:1 molar ratio, succinic anhydride:lysine residues) human thyroglobulin (tg) was prepared by gel filtration on 6% and 4% agarose. this subunit (s-8) had a sdegrees20, w of 7.6s, ddegrees20, w of 2.96 x 10(7) and an equilibrium molecular weight of 165,000 after correction for bound succinic anhydride (sa). the s-8 component was suggested to be a quarter unit of intact tg. the s-8 and intact tg had nearly identical amino acid compositions with differences only in gluta ... | 1978 | 95912 |
| a preliminary study on hepatitis b vaccine. | | 1978 | 95926 |
| bacterial sensitivity to minocycline (minocin) of clinical isolations. | | 1977 | 95945 |
| neuronal activity in the brain stem reticular formation during performance of a "go-no go" visual attention task in the monkey. | | 1978 | 95962 |
| [pseudomonas bacteriophage phikz--possible model for studying the genetic control of morphogenesis]. | a description of structure and some bilogical properties of a new virulent pseudomonas phage, phikz, is given in the paper. phage phikz has unique characteristics of particle (a large size of the head and the presence in the head of a structure which is visible on electron micrographs as crosswise striated substance). a cause of high-titer phage phikz formation on plates has been investigated. characteristics of single step growth experiments, sensitivity of free phage particles to heating, sds- ... | 1978 | 95987 |
| characterization of small plasmids from staphylococcus aureus. | small molecular weight plasmids from staphylococcus aureus were characterized with respect to size, restriction enzyme cleavage pattern and transforming capacity. the plasmids ps194 and pc194 which encode streptomycin and chloramphenicol resistance respectively contained 3.0 and 2.0 megadaltons of dna as determined by zonal rate centrifugation and electron-microscopy. both plasmids transformed s. aureus wigh high efficiency. plasmid pc194 contained only one cleavage site for endonuclease hindiii ... | 1978 | 95989 |
| [gram-positive bacteria as adjuvants (author's transl)]. | gram-positive bacteria or their constituents influence the immune response in various ways. for instance: listeria monocytogenes cellwall fraction acts as b-cell mitogen, in this way enhancing a humoral immune response. corynebacterium parvum acts as a macrophage stimulant evoking maximum macrophage activation. bcg, among other things, leads to an enhancement of a t-cell response to unrelated antigens. the hypothetical therapeutic consequences are discussed. | 1978 | 96006 |
| intracytoplasmic membrane, phospholipid, and sterol content of methylobacterium organophilum cells grown under different conditions. | intracytoplasmic membranes were present in methylobacterium organophilum when cells were grown with methane, but not methanol or glucose, as the sole carbon and energy source. cells grown with methane as the carbon and energy source and low levels of dissolved oxygen had the greatest amount of intracytoplasmic membrane. cells grown with increased levels of dissolved oxygen had less intracytoplasmic membrane. the amount of total lipid correlated with the amount of membrane material observed in th ... | 1978 | 96093 |
| liver microsomal cytochromes p-450 and azoreductase activity. | hepatic microsomal azoreductase activity with amaranth (3-hydroxy-4[(4-sulfo-1-naphthalenyl)azo]-2,7-naphthalenedisulfonic acid trisodium salt) as a substrate is proportional to the levels of microsomal cytochrome p-450 from control or phenobarbital-pretreated rats and mice or cytochrome p-448 from 3-methylchol-anthrene-pretreated animals. in the "inducible" c57b/6j strain of mice, 3-methylcholanthrene and phenobarbital pretreatment cause an increase in cytochrome p-448 and p-450 levels, respect ... | 1978 | 96118 |
| identification and grouping of neisseria meningitidis directly on agar plates by coagglutination with specific antibody-coated protein a-containing staphylococci. | it has been shown that neisseria meningitidis can be grouped by coagglutination directly upon growth on sheep blood or chocolate agar plates. all positive reactions were group specific, and only a single colony was required for a positive reaction. there was variation seen in the effectiveness of commercial antisera in preparing sensitive reagents. certain throat and sputum isolates of group z organisms failed to react by coagglutination, although they would react directly with antiserum in whol ... | 1978 | 96128 |
| immunoglobulin secretion by human splenic lymphocytes in vitro: the effects of antibodies to igm and igd. | the effects of f(ab')2 fragments of affinity-purified rabbit anti-human mu chain antibody (rahmu) and rabbit anti-human delta chain antibody (rahdelta) on spontaneous and mitogen-stimulated immunoglobulin (ig) secretion by normal human spleen cells were studied. igm and igg secretion by human spleen cells cultured in vitro was measured by incubating the cells with 3h-amino acids precipitating the secreted labeled ig with anti-ig, and analyzing the precipitates by sodium dodecyl sulfate polyacryl ... | 1978 | 96179 |
| linkage between a gene for a serum protein and the gene for the ig kappa light chain in rabbits. | a rabbit serum protein (prt) that migrates ahead of transferrin in polyacrylamide gel electrophoresis is inherited in a simple mendelian fashion. the gene for this protein is linked to the gene for the ig kappa light chain and the degree of linkage is calculated as 23.5 +/- 4.7 centimorgans. | 1978 | 96181 |
| alloantigen-induced t helper activity. i. minimal genetic differences necessary to induce a positive allogeneic effect. | addition of histoincompatible lymphocytes can influence the course of ongoing immune responses. such allogeneic effects may either augment or diminish immune responses. we describe here the minimal genetic differences necessary to generate positive allogeneic effects (allohelp) in a humoral immune response. the antibody response to sheep erythrocytes of t cell-depleted mouse spleen cells was reconstituted by addition of syngeneic or allogeneic nylon wool column-passaged spleen t cells. t cells w ... | 1978 | 96182 |
| correlation of neural discharge with pattern and force of muscular activity, joint position, and direction of intended next movement in motor cortex and cerebellum. | 1. monkeys were trained to grasp a rod movable in a horizontal arc (fig. 1), and to hold the rod by angulation of the wrist in each of three positions (a,b, c). a maintained load was placed on the rod alternately to oppose flexion and extension. at a light signal, the monkey had to move to the next position in a prescribed sequence (abcbabcba, etc.). the task was designed to dissociate, while holding in position, the following variables: 1) pattern of muscular activity in the forearm required to ... | 1978 | 96223 |
| functional specificity of lateral geniculate nucleus laminae of the rhesus monkey. | 1. this study investigated the functional specificity of the lateral geniculate mucleus (lgn) of the rhesus monkey using microelectrode-recording techniques. 2. the parvocellular laminae of the lgn receive input predominantly from medium-conduction-velocity optic tract fibers, while the magnocellular laminae receive fast-conducting axons from the retina. 3. cells projecting from the parvocellular layers to area 17 have medium-conduction velocities, while those from the magnocellular layers are f ... | 1978 | 96227 |
| direct administration of methotrexate into the central nervous system of primates. part 1: distribution and degradation of methotrexate in nervous and systemic tissue after intraventricular injection. | levels of methotrexate (mtx) measured by both 3h radioactivity and dihydrofolate reductase assays were determined in cerebrospinal fluid (csf), plasma, urine, and both neural and non-neural tissues at varying times after a single intraventricular injection into cynomolgus monkeys (macaca fascicularis). clearance of the mtx from csf was rapid after injection. a relatively constant level of 3hmtx was reached in plasma 2 1/2 hours after injection, and about 30% of the 3hmtx dose was excreted in the ... | 1978 | 96228 |
| tissue tropism of different trypanosoma cruzi strains. | a systematic study of the distribution of intracellular parasites in the organs and tissues was performed in groups of mice inoculated with 4 different trypanosoma cruzi strains. an extremely high parasitism of spleen, liver, and bone marrow was observed in mice inoculated with y and berenice strains; with cl strain, however, parasites were almost absent in those organs. bloodstream forms apparently present differences which facilitate or prevent their uptake by macrophages from the mononuclear ... | 1978 | 96243 |
| methods for storage of antimicrobial effectiveness test inoculum suspensions below freezing. | studies were conducted to determine the suitability of storage below freezing of some antimicrobial effectiveness test inoculum organisms: staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, and candida albicans. several solutions used for protecting microorganisms subjected to storage below freezing were compared. comparison of 10% dextrose with other solutions (distilled water; 0.07 m phosphate buffer, h 7.0, with 15% glycerol; and 0.07 m phosphate buffer, ph 7.0, with 7.5% dimeth ... | 1978 | 96247 |
| plasma amine oxidase activity in 136 normal rhesus monkeys: relationships between enzyme activity and age, sex and genetic factors. | normative data on plasma amine oxidase activity were obtained for four primate species, m. mulatta, m. arctoides, c. aethiops and h. sapiens. significant between-species differences in activity were observed. in a large sample of m. mulatta, no significant relationship between age and plasma amine oxidase activity was observed, but adult males had significantly higher enzyme activities than adult females. | 1977 | 96261 |
| intrathecal gentamicin for refractory gram-positive meningitis. | the mortality for meningitis due to staphylococcus aureus or listeria monocytogenes remains high, ranging from 32% to 77%. we treated a patient with staphylococcal meningitis refractory to methicillin sodium and oxacillin sodium, and a patient with listeria meningitis refractory to penicillin g potassium and ampicillin sodium; both patients were cured when intrathecal gentamicin sulfate was added to the regimen. the combination of intrathecal gentamicin and a penicillin may be beneficial in refr ... | 1978 | 96281 |
| body weight change and nutritional adequacy in the parenterally alimented rat. | | 1978 | 96303 |
| stage-specific antigens on the surface membrane of sporozoites of malaria parasites. | | 1978 | 96355 |
| [pathogenicity of the indigenous flora of the oral cavity]. | | 1977 | 96398 |
| [the appearance of penicillin resistant gonococci. clinical, genetic and epidemiologic problems]. | | 1978 | 96408 |
| [tuberculin reactions in guinea pigs injected with virulent and avirulent tubercle bacilli]. | | 1978 | 96433 |
| synthesis of cold-insoluble globulin by cultured calf endothelial cells. | radiolabeled amino acids were incorporated into nondialyzable protein by cultures of endothelial cells derived from calf aorta. antibody prepared against purified bovine plasma cold-insoluble globulin (cig) formed a strong precipitin line upon immunodiffusion against 3h-labeled proteins from endothelial cell culture media. this precipitin line formed a line of identity with the precipitin line formed by anti-cig and purified cig. double-antibody immunoprecipitation of 3h-labeled protein showed t ... | 1978 | 96438 |
| clusters of intramembranous particles on cultured myotubes at sites that are highly sensitive to acetylcholine. | electrophysiological and autoradiographic studies have shown that the distribution of acetylcholine (accho) receptors on uninnervated cultured chicken muscle cells is not uniform. regions of high receptor density (hot spots 10--40 times more sensitive than surrounding areas are localized as discrete patches or clusters about 10 micrometer in diameter o myotube muscle membranes. hot spots were also found on fusion-arrested mononucleated myoblasts. we have developed a method for freeze-fracturing ... | 1978 | 96446 |
| involvement of gamma-amino butyric acid (gaba) in the anticonvulsant action of methaqualone. | the effects of methaqualone on isonicotinic acid hydrazide, 6-mercapto propionic acid, picrotoxin, and strychnine-induced convulsion were studied in mice and the results compared with diazepam. methaqualone, like diazepam, was found to be a selective antagonist of isoniazid-induced convulsion and a much less effective inhibitor of strychnine convulsion. methaqualone elicits muscle-relaxant, sedative, and anticonvulsant effects at different dose levels. at low, nonsedative doses the drug produces ... | 1978 | 96460 |
| the caloric and intoxicating properties of fluid intake as components of stress-induced ethanol consumption in rats. | rats housed continuously in a test environment for 25 days were offered water, 5% v/v ethanol, 10% v/v ethanol, and propylene glycol at 7.5% w/v. the propylene glycol concentration represented a caloric midpoint between the 5 and 10% ethanol. after 10 baseline sessions, during which preference for the four solutions was shown to be statistically equal, shock schedules were introduced. the consumption of ethanol at both concentrations showed significant peaks for the interval immediately followin ... | 1978 | 96465 |
| deprenyl administration in man: a selective monoamine oxidase b inhibitor without the 'cheese effect'. | after pretreatment with the selective monoamine oxidase b inhibitor, (-)-deprenyl, in doses sufficient for complete inhibition of the platelet enzyme, 4 normal and 6 parkinsoniam volunteers (2 receiving levodopa and 2 levodopa plus carbidopa) suffered no adverse pressor reaction ('cheese effect') after challenge with oral tyramine in amounts considerably greater than those likely to be encountered in a normal diet. nor did the levodopa-deprenyl combination itself result in a pressor response. no ... | 1978 | 96466 |
| purification and immunochemical quantitation of human pancreatic secretory trypsin inhibitor. | human pancreatic secretory trypsin inhibitor has been purified from pancreatic juice obtained on connection with surgery in the pancreas. the purification was accomplished by gel filtration on sephadex g-75 and ion-exchange chromatography on sp-sephadex c-50 giving 49% yield. five forms of the inhibitor were demonstrated by combined chromatographic and electrophoretic methods. on gel filtration the inhibitor was shown to have the same molecular weight as the kunitz inhibitor, i.e. about 6500. in ... | 1978 | 96521 |
| [diagnosis of vibriosis in bulls at breeding stations through lavages of the artificial vagina]. | the objective of the study was to test the suitability of lavages of artificial vaginas which are used for collecting ejaculate for diagnosing the campylobacter fetus subsp. fetus germs. according to the results of the investigation this method may be considered equal to the method of lavages of the prepuce. however, it is important to point out the advantages: it eliminates the danger of an accident, it enables quality lavage in the laboratory. to caputure the campylobacter fetus the filtration ... | 1978 | 96573 |
| [effectiveness of antigens from various developmental stages of ascaris suum in the deomonstration of the migratory phase of experimental ascariasis in pigs]. | tests of the efficiency of antigens prepared from different developmental stages of ascaris suum in indirect haemagglutination test in the course of proving the migration phase of experimental ascariasis in pigs show that the antigens prepared by ultrasound from the invasive larval stage of a. suum in comparison with antigens of sexually mature stages have higher serological activity. by using this antigen it is possible to prove specific antibodies in experimentally invaded pigs from 6 to 120 d ... | 1978 | 96575 |
| [the technic of arterial blood-specimen collection in cattle]. | a simple method of collecting arterial blood in cattle by puncture of the arteria iliaca externa per rectum is described. individual collections do not induce any local reaction nor do they impair the overall condition and milk yield of the animals. | 1978 | 96577 |
| [application of frozen stored various animal red blood cells for the virological and serological tests (author's transl)]. | | 1977 | 96589 |
| pituitary-adrenal response to stimulation of the limbic system and lateral hypothalamus in the rhesus monkey (macacca mulatta). | various sites within the limbic system, frontal lobe and lateral hypothalamus of the rhesus monkey brain were electrically stimulated using chronically implanted electrodes. increases in plasma cortisol was observed after stimulation of the lateral hypothalamic area, basolateral amygdala, pyriform cortex, hippocampus, certain sites in the cingulate gyrus and orbital part of the frontal lobe. inactive sites included the caudate nucleus, putamen and white matter in the frontal lobe. | 1978 | 96644 |
| the role of phosphatidylglycerol as a donor of phosphatidyl and of sn-glycerol-1-phosphate groups in biosynthetic reactions. | | 1978 | 96663 |
| inhibitors of beta-glucosidases of animal tissues. | | 1978 | 96666 |
| the hla complex in 1977. | | 1977 | 96678 |
| differential binding of penicillin by membrane fractions from penicillin-susceptible and -resistant gonococci. | an inverse relationship between binding of [(14)c]penicillin to cells of neisseria gonorrhoeae and the minimal inhibitory concentration of penicillin was recently reported by us. herein we report a similar relationship in binding by cytoplasmic membranes derived from 10 strains of gonococci. membranes were extracted after braun homogenization and differential centrifugation of the resulting extracts. membranes derived from penicillin-susceptible cells bound 140 to 200 nmol of [(14)c]penicillin p ... | 1978 | 96727 |
| unpredictable response of pseudomonas aeruginosa to synergistic antibiotic combinations in vitro. | the combined activity of ticarcillin and each of three aminoglycosides, gentamicin, tobramycin, and amikacin, was studied with 45 clinical isolates of pseudomonas aeruginosa. all strains individually were susceptible to each of these agents. comparison of the results revealed highly variable interaction, both from strain to strain and from one aminoglycoside to another against the same strain. against 11 strains, none of three antibiotic pairs tested was synergistic by conventional criteria; aga ... | 1978 | 96736 |
| antibiotic susceptibility of clinical isolates of listeria monocytogenes. | a broth microdilution method was used to measure the minimal inhibitory concentrations (mics) of the antibiotics most often recommended for treatment of listeriosis. the mics of ampicillin, penicillin, erythromycin, and tetracycline for 175 strains of listeria monocytogenes were below the approximate mic breakpoint for susceptible strains as recommended by the national committee on clinical laboratory standards. inhibition diameters for 125 strains were measured by the standardized disk method ( ... | 1978 | 96737 |
| portal hypertension and chronic salmonellosis in situs invertus. | the unique combination of a late complication of schistosoma mansoni infection in situs invertus is reported. the improvement in both the haematological and biochemical indices of the patient after splenectomy is discussed. an association between chronic salmonellosis and schistosomiasis is demonstrated; the experience of the author and other workers with this syndrome is discussed. | 1978 | 96743 |
| metabolism of sulpiride in man and rhesus monkeys. | the metabolism of 14c-carbonyl-sulpiride (form a) and of 14c-3, 4 pyrrolidine-sulpiride (form b) was studied in the rhesus monkey and man. in the monkey, the metabolites in both the urine and the bile were the same with form a and form b: 60-80% sulpiride, 10-30% 5-oxopyrrolidine sulpiride and 3-8% an unidentified metabolite (me-x). in four human volunteers given a single oral dose of either 108 mg form a or 100 mg form b, more than 95% of the 14c recovered in the urine and feces was unchanged s ... | 1978 | 96745 |
| efficacy of penicillin g, cefazolin, and gentamicin in m-k medium at 4 degrees c. | corneal-scleral shells are routinely stored in mccarey-kaufman (m-k) medium at 4 degrees c. antibiotics are added to the medium to suppress bacterial growth. we examined the antibacterial activity of penicillin g potassium, 100 microgram/ml, cefazolin sodium, 100 microgram/ml, and gentamicin sulfate, 20 microgram/ml against test organisms in m-k medium at 4 degrees, 23 degrees, and 37 degrees c for periods up to 18 hours. within the limits of the experimental conditions, none of the antibiotics ... | 1978 | 96782 |
| separation of oestrone udp-glucuronyltransferase and p-nitrophenol udp-glucuronyltransferase activities. | rabbit liver udp-glucuronyltransferase activity was resolved into two separate fractions on deae-cellulose, one containing most of the transferase activity toward oestrone and the other most of the activity toward p-nitrophenol. these two activities were completely separated by rechromatography of each fraction on a second deae-cellulose column. | 1978 | 96815 |
| interaction of the nitrogenase components of anabaena cylindrica with those of clostridium pasteurianum. | | 1978 | 96817 |
| analyses of absorption and fluorescence spectra of water-soluble chlorophyll proteins, pigment system ii particles and chlorophyll a in diethylether solution by the curve-fitting method. | absorption and fluorescence spectra in the red region of water-soluble chlorophyll proteins, lepidium cp661, cp663 and brassica cp673, pigment system ii particles of spinach chloroplasts and chlorophyll a in diethylether solution at 25 degrees c were analyzed by the curve-fitting method (french, c.s., brown, j.s. and lawrence, m.c. (1972) plant physiol 49, 421--429). it was found that each of the chlorophyll forms of the chlorophyll proteins and the pigment system ii particles had a correspondin ... | 1978 | 96855 |
| phycobiliprotein synthesis in protoplasts of the unicellular cyanophyte, anacystis nidulans. | stable and metabolically active protoplasts were prepared from the unicellular cyanophyte, anacystis nidulans, by enzymatic digestion of the cell wall with 0.1% lysozyme. the yield of protoplasts from intact algal cells was approx. 50%. incorporation of l-[u-14c]leucine into cold trichloroacetic acid-insoluble material from protoplasts preparations was linear for 1.5 h and continued for an additional 2.5 h. incorporation of radiolabeled leucine into hot trichloroacetic acid-insoluble material fr ... | 1978 | 96857 |
| [effect of combined administration of hydrocortisone and edta on the number of the antibody-producing cells in the spleen of mice immunized with sheep erythrocytes]. | experiments on mouse hybrids (cba x c57bl)f1i indicated that injection of hydrocortisone in a dose of 1 mg/mouse 24 hours after the immunization with sheep red blood cells against the background of multiple edta injection resulted in a relative reduction of the plaque-forming cells in the spleen--more than 6-fold in comparison with control, and more than 3-fold in comparison with the effect of hydrocortisone or edta alone. this may possibly be the consequence of a more intensive hydrocortisone i ... | 1978 | 96887 |
| fibrinogen and adp-induced platelet aggregation. | | 1978 | 96891 |
| changes in responsiveness of medial preoptic neurons to the microelectrophoresis of releasing hormones as a function of ovarian hormones. | | 1978 | 96913 |
| the primary structure of staphylococcal protease. | the amino acid sequence of staphylococcal protease has been determined by analysis of tryptic peptides obtained from cyanogen bromide fragments. selected peptides obtained from digests with staphylococcal protease, thermolysin, and chymotrypsin provided the information necessary to align the tryptic peptides and the cyanogen bromide fragments. the protease is a single polypeptide chain of some 250 amino acids and is devoid of sulfhydryl groups. the cooh-terminal tryptic peptide of of the proteas ... | 1978 | 96922 |
| spinal reflexes as a basis for tremor in the premammillary cat. | a maintained tremor observed in premammillary cats has been studied by varying the load attached to the soleus muscle, by electrical recording, and stimulation. the frequency and amplitude of the tremor varies widely with changes in elastic or inertial loading (springs or flywheels). these variations agree well with predictions based on the idea that the tremor is maintained by relfexes reinforcing a tendency for oscillation in a mass-spring system (which includes the elasticity of the muscle). ... | 1978 | 96926 |
| test for differentiation of m. tuberculosis and m. bovis from other mycobacteria. | a test is described for selective inhibition of mycobacterium tuberculosis and m. bovis isolates in fluid medium. the method employs rho-nitro-alpha-acetyl-amino-beta-hydroxy-propiophenone (nap) as an inhibitory agent for differentiation of mammalian tuberculosis strains from other mycobacteria. | 1978 | 96930 |
| analysis of cytotoxic mechanisms induced by minor histocompatibility antigens in the mouse. | | 1978 | 96941 |
| the rabbit pulmonary monooxygenase system: characteristics and activities of two forms of pulmonary cytochrome p-450. | two forms of rabbit pulmonary cytochrome p-450 have been characterized spectrally and their activities in reconstituted monooxygenase systems investigated. the presence of both microsomal phospholipids and sodium cholate was required to obtain optimum activity. only one of the cytochromes (i) was active in the n-demethylation of benzphetamine and the o-deethylation of 7-ethoxycoumarin. however, cytochrome ii was 20% more active than cytochrome i in the metabolism of benzo[a]pyrene. the profile o ... | 1978 | 96944 |
| [chemical reactivity and biological properties of a series of aminochlorambucil derivatives]. | the substitution of aminochlorambucil by a methyl group increased the chemical reactivity in iv b (n = 1) and iv b (n = 2) but their cytotoxicity remained low. the immunosuppressive effect (adjuvant arthritis in rats and tuberculin related skin reaction) was observed with iv b (n = 2). aminochlorambucil was effective on adjuvant arthritis only and iv b (n = 1) had no activity. aminochlorambucil, iv b (n = 1) and iv b (n = 2) were devoid of any non-specific anti-inflammatory activity. | 1978 | 96958 |
| [antimicrobial activities of two strains of streptomyces albus isolated from african soils]. | the antibiotic activity of these two strains of s. albus is facilitated by culture on slices of potatoes. streptomyces extracts show an antibiotic activity against gram-positive and gram-negative bacteria and an antifongic activity aginst nearly all aspergillus. these antimicrobial effects bring hope of a utilization against some mycotoxin pollution, or mold attacks. | 1978 | 96972 |
| [dna polymerases in human platelets]. | human platelets have two dna polymerases. in the mitochondrial-free cytoplasm we have found a dna polymerase gamma and another dna polymerase very closely related to the polymerase alpha from animal cells. in the mitochondria only the gamma activity is present. | 1978 | 96974 |
| [isolation of bacteria that use that use nitric oxide as a respiratory electron acceptor under anaerobiosis]. | ten bacteria of the genus bacillus were isolated from pasteurized soils, in anaerobiosis and at 32 degrees c, on peptone broth containing 0.5% kno2. they are gram variable rods producing oval spores. they are oxidase positive and have catalase. they grow, in anaerobiosis, on no-3, no-2, n2o, and no as respiratory electron acceptors. these compounds are reduced to n2. | 1978 | 97003 |
| [cutaneous respiration in a selachian (scyliorhinus caniculus l.)]. | cutaneous oxygen consumption (mo2) and cutaneous oxygen uptake from the external medium were investigated in an elasmobranch scyliorhinus caniculus l. in spite of an important mo2 (2.75 +/- 0.28 nmol.mn-1 cm-2 ; n=6) a positive net flux of oxygen through the skin takes place (2.98 +/- 0.33 nmol. mn-1 .cm-2). the skin of the dogfish in an exchanger for oxygen supplying 10% of oxygen requirements of other organs of the body in these experimental conditions (t = 13 degrees c). | 1978 | 97014 |
| [demonstration of testosterone secretion by testicular tissue of hypophysectomized boar as affected by hcg in organ culture]. | boar leydig cells undergo a strong atrophy from 1 to 3 months after hypophysectomy but can be reactivated by the gonadotropin hcg in organ culture conditions. this reactivation which appeared at histological and ultrastructural level was evidenced by the capacity of testicular tissue to synthesize testosterone as judged by radioimmunoassay. both synthesis in the tissue and release into the medium increased according the incubation time with hcg; the adjonction of 17 alpha-oh-pregneolone to cultu ... | 1978 | 97016 |
| baroreceptor reflexes in human hypertension. | we studied the control of arterial pressure by the carotid sinus baroreceptors in 35 hypertensive humans, using a variable pressure neck chamber to alter carotid sinus transmural pressure in a graded fashion. the results were compared with those obtained from 11 normotensives. as in normotensives, reduction in carotid transmural pressure caused a linearly related pressor response and vice versa. however, whereas in normotensives the pressure response was greater than the depressor, the reverse w ... | 1978 | 97032 |
| thyroid function in children with growth hormone deficiency, either idiopathic or caused by diseases of the central nervous system. | thyroid function was assessed in thirty two patients with growth hormone deficiency (ghd) by clinical examination and by measurement of t4-levels, free t4-indices, basal tsh values and tsh responses to trh (100 mu/m2). sixteen patients (50%) were hypothyroid. in thirteen patients, the endocrine disorders were considered to be of hypothalamic origin. ten of them showed prolonged responses to trh and in the other three the responses were exaggerated. in three patients hypothyroidism was due to a p ... | 1978 | 97084 |
| monoclonal igm cryoglobulinemia associated with gamma-3 heavy chain disease: immunochemical and biochemical studies. | a patient (mia) with a monoclonal igm(kappa) cryoglobulin (cryo igm) developed additional heavy chain disease proteins of the gamma3 subclass 8 years later. biochemical studies of the cryo igm indicated that the heavy chain was vhi, but the nh2-terminal amino acid sequence of the light chain did not permit a definite assignment of its vkappa subgroup. two major fragments of the gamma3 chain were distinguishable by electrophoresis in sodium dodecyl sulfate polyacrylamide gel. the smaller componen ... | 1978 | 97087 |
| lysosomal acid hydrolases of normal adipose tissues of man, monkey and rat. | | 1978 | 97094 |
| effects of chronic and acute treatment with sulfonylureas on plasma insulin and glucose levels in the rat. | | 1978 | 97105 |
| cytogenetics and taxonomy of some south bolivian monkeys. | illustrations are given by the authors of chromosome banding patterns of three species of monkeys: douroucouli, black-capped capuchin and squirrel monkey. the precise locality of the animals in southern bolivia is known. this enables a taxonomic discussion on the value of some names of these animals. | 1978 | 97192 |
| the use of intramuscularly administered methyl-trh to evaluate the pituitary-thyroid axis. | the present study was carried out to evaluate the effectiveness of intramuscular administration of methyl-trh, a potent analogue of thyrotropin-releasing hormone, for assessing pituitary reserve of tsh and prolactin and for distinguishing euthyroid, hypothyroid and hyperthyroid individuals. serum samples were taken for 24 hours after intramuscular injection of methyl-trh, 200 microgram, in 19 euthyroid subjects, 9 hypothyroid men and 9 hyperthyroid men. the mean serum prolactin and tsh concentra ... | 1978 | 97198 |
| absorption of dieldrin through monkey & dog skin. | | 1977 | 97210 |
| impaired host resistance to endotoxin and malaria in polychlorinated biphenyl- and hexachlorobenzene-treated mice. | the in vivo effect of polychlorinated biphenyl (pcb) and hexachlorobenzene (hcb) on murine endotoxin sensitivity and resistance to malaria (plasmodium berghei nyu-2) infection was studied. the dietary administration of 167 ppm (167 microgram/g) of pcb 1242 or hcb for 3 weeks resulted in an enhanced sensitivity to gram-negative endotoxin (salmonella typhosa), which was further increased in animals maintained on the diets for 6 weeks. by 6 weeks, a 5.2- or 32-fold increase in endotoxin sensitivity ... | 1978 | 97225 |
| listeria monocytogenes cell walls induce decreased resistance to infection. | a significant decrease in murine resistance to listeria monocytogenes was induced by using crude listeria cell wall fraction (lcwf) and purified listeria cell walls (pf). when equal amounts of these materials were injected, pf was more effective than lcwf in decreasing resistance. the pf effect was dose dependent when measured either as a decrease in 50% lethal dose of the listeria challenge or as a decrease in survival time of the infected mice. pf apparently does not act directly on the lister ... | 1978 | 97226 |
| induction of capping in blood-stage trypomastigotes of trypanosoma cruzi by human anti-trypanosoma cruzi antibodies. | the effect of human-specific antibody on surface membrane antigens of trypomastigotes of trypanosoma cruzi was studied in vitro by using immunofluorescence methods. immune sera induced aggregation of surface antigens in trypomastigotes to form polar cell caps. | 1978 | 97230 |
| adjuvant activity of synthetic 6-o-"mycoloyl"-n-acetylmuramyl-l-alanyl-d-isoglutamine and related compounds. | adjuvant and antitumor activities of synthetic 6-o-"mycoloyl"-n-acetylmuramyl-l-alanyl-d-isoglutamine were examined. all the synthetic 6-o-corynomycoloyl-, 6-o-mocardomycoloyl-, and 6-o-mycoloyl-n-acetylmuramyl-l-alanyl-d-isoglutamine were active as adjuvants for cell-mediated immune responses. however, 6-o-mycoloyl-n-acetylmuramyl-l-alanyl-d-isoglutamine was less active as an adjuvant on circulating antibody formation. it was shown that pyrogenic activity of n-acetylmuramyldipeptide was reduced ... | 1978 | 97234 |
| synthesis and preliminary scintigraphic evaluation of in vivo distribution of c-lactic acid and c-lactonitrile. | | 1978 | 97239 |
| metabolism of bile acids. v . metabolism of ursodeoxycholic acid in rhesus monkey. | | 1977 | 97247 |
| is the solitary dark neuron a manifestation of postmortem trauma to the brain inadequately fixed by perfusion? | dark neurons, classified as solitary because of their sparse occurrence, were discerned in the transitional zones between gray and white matter in various species of laboratory animals fixed by perfusion. these neurons, histologically indistinguishable from dark neurons in immersion fixed material, tended to develop when the saline perfusion was delayed or slow, the amount of the bouin fixative was excessive, or the autopsy was performed shortly after the perfusion. under these conditions, the w ... | 1978 | 97249 |
| influence of type of antibiotic and length of antibiotic feeding period on performance and persistence of antibiotic resistant enteric bacteria in growing-finishing swine. | | 1978 | 97254 |
| antimicrobial agents as preservatives in pharmaceutical and cosmetic products. agents as preservatives in eye-drops and contact lens solutions. | | 1978 | 97255 |
| the use of ferret trachea organ cultures for therapeutic studies of anti-influenzal drugs. ii. comparison of four compounds in tissue culture and in organ culture. | | 1978 | 97260 |
| activity of amikacin against mycobacterium tuberculosis. | | 1978 | 97261 |
| ultrastructural study of salmonella typhimurium treated with membrane-active agents: specific reaction dansylchloride with cell envelope components. | amino groups of cell envelope proteins, lipids, and lipopolysaccharides cannot be labeled in intact cells of salmonella typhimurium g 30 by using 5-dimethylaminonaphthalene-1-sulfonylchloride incorporated in lecithin-cholesterol vesicles. however, application of membrane-interacting agents like tris(hydroxymethyl)aminomethane (tris)-hydrochloride, ethylenediaminetetraacetate (na salt) (edta), divalent cations, and sublethal doses of the cationic antibacterial agents polymyxin b and chlorhexidine ... | 1978 | 97268 |
| biosynthesis of delta-aminolevulinic acid by blue-green algae (cyanobacteria). | when levulinic acid, a competitive inhibitor of delta-aminolevulinic acid dehydratase, was added to growing cultures of blue-green algae (cyanobacteria), delta-aminolevulinic acid was excreted into the medium and cell growth was inhibited. | 1978 | 97274 |
| polyethylene glycol-induced fusion of heat-inactivated and living protoplasts of bacillus megaterium. | protoplasts of bacillus megaterium, incubated at 50 degrees c for 120 min, lost the ability to revert to bacillary form. such heat-inactivated protoplasts, however, produced recombinants when fused by polyethylene glycol treatment with normal protoplasts. although this differential inactivation effect is not yet fully reproducible, reciprocal inactivations of the parental protoplasts in genetic crosses have clearly shown that for protoplast fusion (i) either of the parents may serve as the viabl ... | 1978 | 97276 |
| independence of proline chemotaxis and transport in bacillus subtilis. | values of ki for nine proline analogs as inhibitors of proline chemotaxis and of proline transport were determined. two of them inhibited transport at substantially lower concentrations than chemotaxis; two at substantially higher concentrations. moreover, mutants, believed to be in the component that binds proline, were isolated that showed a shift of km for transport to higher concentrations, one as much as 40-fold. however, chemotaxis was virtually unaffected. therefore, unlike galactose chem ... | 1978 | 97283 |
| improved version of the exoantigen test for identification of coccidioides immitis and histoplasma capsulatum cultures. | a rapid and simple method for extracting specific cell-free antigens of coccidioides immitis and histoplasma capsulatum from agar slant cultures was developed. the extracts were analyzed in immunodiffusion tests for the presence of c. immitis or h. capsulatum specific antigens. these extracts were compared with culture filtrates of brain heart infusion broth subcultures in tests with 32 isolates of c. immitis and h. capsulatum and 13 other fungi which might be morphologically confused with them. ... | 1978 | 97310 |
| isolation and analysis of h-2 and ia alloantigens from wild mouse strains. | immunoprecipitation and sds-page analysis were used to examine h-2 and ia antigens from mouse strains with wild-derived mhc haplotypes. antisera raised against the wild-derived strains contained anti-h-2 and anti-ia antibodies which precipitated antigen molecules readily distinguishable by a single assay procedure. the antibodies to wild strains were cross-reactive with standard laboratory haplotypes. evidence supporting the similar genetic organization of wild and standard haplotypes was found, ... | 1978 | 97344 |
| elimination of inter-species reactive anti-igg antibodies by affinity chromatography. | inter-species reactive anti-igg antibodies are disturbing in blocking and interference experiments with the sensitive mixed hemadsorption (mh) technique. by affinity chromatography this disturbance could be efficiently eliminated. anti-igg sera were passed through columns containing heterologous igg. the absorption technique is rapid and simple. after one absorption the inter-species reactivity in anti-igg sera had been decreased thousand-fold, while any loss of species-specific reactivity could ... | 1978 | 97348 |
| lymphocyte responsiveness to pseudomonas aeruginosa in cystic fibrosis: relationship to status of pulmonary disease in sibling pairs. | lymphocyte proliferative responses to pseudomonas aeruginosa and staphylococcus aureus were evaluated in six sibling pairs with cystic fibrosis. in each pair, one sibling had advanced clinical disease, whereas the other sibling was in good clinical condition. three in this latter group had no clinically apparent pseudomonas bronchitis. in all cases, the average responses to pseudomonas isolated from each sibling pair were lower in the sibling with advanced clinical disease. this difference was n ... | 1978 | 97373 |
| folate antagonists. 12. antimalarial and antibacterial effects of 2,4-diamino-6-[(aralkyl and alicyclid)thio-, sulfinyl-, and sulfonyl]quinazolines. | a series of 2,4-diamino-6-[(aralkyl and alicyclic)thio-, sulfinyl-, and sulfonyl]quinazolines was prepared via condensation of 5-chloro-2-nitrobenzonitrile or 5,6-dichloro-2-nitrobenzonitrile with the appropriate aralkyl or alicyclic thiopseudourea, reduction of the resulting 2-nitro-5-[(aralkyl or alicyclic)thio]benzonitrile with stannous chloride to the amine, and cyclization with chloroformamidine hydrochloride. oxidation was effected with hydrogen peroxide or the bromine complex of 1,4-diaza ... | 1978 | 97382 |