| deferoxamine inhibition of malaria is independent of host iron status. | the mechanism whereby deferoxamine (df) inhibits the growth of malaria parasites was studied in rats infected with plasmodium berghei. peak parasitemia was 32.6% (day 14) in untreated controls and 0.15% (day 7) in rats receiving 0.33 mg/g in 8 hourly df injections, subcutaneously. df inhibition of parasite growth was achieved without any reduction in transferrin saturation or hemoglobin synthesis and with only a partial (56%) depletion of hepatic iron stores. dietary iron depletion resulted in a ... | 1988 | 3294334 |
| evaluation of the skin disinfecting activity and cumulative effect of chlorhexidine and triclosan handwash preparations on hands artificially contaminated with serratia marcescens. | the initial and cumulative efficacy of two antiseptic handwash preparations in eliminating serratia marcescens from hands was evaluated on volunteers. two antiseptics with persistent skin antibacterial activity, 4% chlorhexidine gluconate in detergent and 1.5% triclosan in natural soap, were studied in a new protocol designed according to food and drug administration guidelines. after a single handwash, both preparations exhibited a degerming action statistically superior to the mechanical elimi ... | 1987 | 3294700 |
| [gardnerella vaginalis in nonspecific vaginitis]. | | 1987 | 3296818 |
| radiation inactivation analysis of enzymes. effect of free radical scavengers on apparent target sizes. | in most cases the apparent target size obtained by radiation inactivation analysis corresponds to the subunit size or to the size of a multimeric complex. in this report, we examined whether the larger than expected target sizes of some enzymes could be due to secondary effects of free radicals. to test this proposal we carried out radiation inactivation analysis on escherichia coli dna polymerase i, torula yeast glucose-6-phosphate dehydrogenase, chlorella vulgaris nitrate reductase, and chicke ... | 1987 | 3298256 |
| comparison of enzyme-linked immunosorbent assay and complement fixation and indirect fluorescent-antibody tests for detection of coxiella burnetii antibody. | an enzyme-linked immunosorbent assay (elisa) was developed to detect immunoglobulin g to coxiella burnetii phase ii. serum samples from 213 patients who had had q fever 1 year previously and from 301 blood donors from six localities in switzerland were tested by elisa and by indirect fluorescent-antibody (ifa) and complement fixation (cf) tests. the elisa and the ifa and cf tests detected antibody to c. burnetii in 202 (94.8%), 193 (90.6%), and 166 (77.8%) of the 213 q fever patients, respective ... | 1987 | 3298311 |
| an important outbreak of human q fever in a swiss alpine valley. | we describe an important q fever human epidemic involving 415 serologically confirmed cases in the val de bagnes (valais, switzerland) during the autumn of 1983. the ages of the 415 cases with acute q fever ranged from 8 to 82 years with a mean of 35 years, of whom 58% (240) were men and 42% (175) were women. this outbreak started about three weeks after 12 flocks of sheep (between 850 and 900 animals) descended from the alpine pastures to the valley. geographically, the epidemic reached all the ... | 1987 | 3301708 |
| chlamydial hemagglutinin identified as lipopolysaccharide. | chlamydial lipopolysaccharide (lps) agglutinated mouse and rabbit erythrocytes but not human, guinea pig, or pronghorn antelope erythrocytes. hemagglutination was not specific for chlamydia spp., as rough lpss from coxiella burnetii and escherichia coli also agglutinated erythrocytes from the same animal species. nonagglutinated and agglutinated erythrocytes bound equivalent amounts of lps, indicating that hemagglutination was not due to a specific interaction of chlamydial lps with erythrocytes ... | 1987 | 3301820 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 37-1987. a 50-year-old man with bilateral pneumonia and respiratory failure. | | 1987 | 3306377 |
| [the prevalence of chlamydia trachomatis infections in a general hospital in amsterdam]. | | 1987 | 3309684 |
| [simultaneous detection by immunofluorescence of several viral infections and mycoplasma, chlamydia and rickettsia burneti infections localized at the level of the conjunctival, nasal and pharyngeal mucosae of patients with recurring chronic keratoconjunctivitis]. | a study was conducted of the viral and inframicrobial populations present in the conjunctival, nasal and pharyngeal mucous membranes by immunofluorescence techniques, using a twelve hyperimmune serum kit, in 120 patients with chronic, relapsing keratoconjunctivitis. qualitative and quantitative differences were noted in the antigen levels in the three investigated mucous membranes. the number of antigens in the conjunctival and nasal epithelial cells was higher than in the pharyngeal ones: 117 a ... | 1987 | 3310378 |
| [theoretical analysis of h+/o stoichiometry during aerobic oxidation in e. coli in the stationary state]. | a general scheme of e. coli respiratory chain under aerobic oxidation with nad.h2 is considered. the ratio h+/o is calculated by the currents method for the respiratory chain in a stationary state. the maximal possible stoichiometry is shown to equal 8. the origin of different h+/o values in the respiratory chains is discussed. | 1987 | 3311170 |
| measurement of regional myocardial perfusion and mass by subselective hydrogen infusion and washout techniques: a validation study. | a technique was developed for measuring regional myocardial perfusion by intracoronary infusions of hydrogen (h2)-saturated saline. h2 concentration was detected during washout in the pulmonary artery by means of the voltage response of a platinum-tipped electrode. regional myocardial perfusion was calculated from the h2 exponential desaturation curve according to the kety-schmidt principle. in 16 anesthetized open-chest dogs, validation of this technique was performed at baseline, reduced (sten ... | 1987 | 3315296 |
| measurement of regional myocardial perfusion and mass by subselective hydrogen infusion and washout techniques: a validation study. | a technique was developed for measuring regional myocardial perfusion by intracoronary infusions of hydrogen (h2)-saturated saline. h2 concentration was detected during washout in the pulmonary artery by means of the voltage response of a platinum-tipped electrode. regional myocardial perfusion was calculated from the h2 exponential desaturation curve according to the kety-schmidt principle. in 16 anesthetized open-chest dogs, validation of this technique was performed at baseline, reduced (sten ... | 1987 | 3315296 |
| prosthetic valve endocarditis. | about 2% of patients with a prosthetic valve will develop endocarditis. this may occur within a few weeks of the valve replacement operation (early) or many months or years later (late). the infecting organisms, pathogenicity and prognosis differ in the two groups. the incidence of early prosthetic valve endocarditis (pve) is under 1%; the predominant organisms are staphylococci that are acquired in the operating theatre or in the intensive therapy unit. early pve usually follows wound sepsis th ... | 1987 | 3316160 |
| aetiology and epidemiology of infective endocarditis in england and wales. | comparing data relating to the estimated incidence of infective endocarditis and deaths due to this condition highlights the changing age and sex patterns over the last 50 years. using these data it is estimated that about 20 cases per million population per year can now be expected many of whom will be aged 65 years or more. about 20% of cases die. viridans streptococci are responsible for almost half the cases of endocarditis, faecal streptococci for 11%, staphylococci for 20% and coxiella bur ... | 1987 | 3316164 |
| q fever: current concepts. | persons with q fever usually present with severe retrobulbar headache, a fever to 104 degrees f or higher with shaking chills, general malaise, myalgia, chest pain, and sometimes pneumonia and hepatitis. cattle, sheep, goats, and ticks are the primary reservoirs of the etiologic agent, coxiella burnetii. humans are usually infected by inhaling infectious aerosols. because c. burnetii can survive for long periods in the environment, it poses a continuing health hazard once it is disseminated. q f ... | 1987 | 3317737 |
| pulsatile secretion of growth hormone and insulin in relation to feeding in rats. | in unrestrained male wistar rats chronically implanted with intracardiac catheters, blood samples were taken every 20 min throughout the 24 h of the diurnal cycle. plasma concentrations of growth hormone (gh), insulin, and glucose were measured. the pattern of food intake was continuously monitored. the existence of 3-h pulsatile cycles of gh secretion was confirmed. in addition, short bursts of insulin secretion were observed in the middle of every second gh peak-to-peak interval. food intake a ... | 1987 | 3318507 |
| antibodies against mammary derived growth inhibitor (mdgi) react with a fibroblast growth inhibitor and with heart fatty acid binding protein. | a polypeptide growth inhibitor, designated as mammary derived growth inhibitor, has previously been purified from lactating bovine mammary glands. polyclonal rabbit antiserum raised against mammary derived growth inhibitor cross-reacts with bovine heart fatty acid binding protein and bovine peripheral myelin p2 protein. these results are consistent with the observation that the amino acid sequence of mammary derived growth inhibitor showed homology to the sequences of these proteins. in a parall ... | 1987 | 3318832 |
| treatment of respiratory tract infections with cephalosporin antibiotics. | infections of the respiratory tract are among the most common causes for antibiotic prescribing. their diagnosis within the community is generally limited to clinical criteria, and microbiological information is frequently lacking. hospitalised patients with respiratory tract infections are more likely to undergo diagnostic sampling, but difficulties remain in reliably defining a microbial aetiology, thereby providing a confident basis for antibiotic selection. in considering the role of the cep ... | 1987 | 3319501 |
| q fever in europe: current aspects of aetiology, epidemiology, human infection, diagnosis and therapy. | | 1987 | 3319911 |
| role of bradykinin generating and degrading systems in the vascular permeability response induced with kaolin in rats. | we investigated how bradykinin mediates inflammatory reactions in rats, via measurements of bradykinin by enzyme immunoassay method in inflammatory tissue fluids. vascular permeability was increased markedly during the first 10 min and then declined quickly after the infusion of a kaolin suspension (10 mg/ml) in 0.8% carboxymethl-cellulose solution into an air pouch formed on the back of rats. bradykinin in the exudate reached a maximum 5 min after the challenge and then decreased quickly. local ... | 1987 | 3320343 |
| evidence that dna involved in the expression of nodulation (nod) genes in rhizobium binds to the product of the regulatory gene nodd. | in rhizobium leguminosarum biovar viciae, the regulatory nodulation nodd gene has at least two functions. it constitutively represses its own transcription and in the presence of inducer flavonoid molecules, it activates the expression of two other nod gene transcriptional units, nodabcij and nodfe. upstream of noda and nodf is a conserved sequence, the nod box, which has been implicated in nodd-mediated transcriptional activation of these genes. dna fragments spanning the nod boxes that precede ... | 1987 | 3320955 |
| insulin stimulates ammoniagenesis in canine renal proximal tubular segments. | to characterize the effect of insulin on ammoniagenesis in renal proximal tubule, ammonia productions were measured in suspensions of canine renal proximal tubular segments incubated with 10 mm l-glutamine and varying concentrations of insulin. productions of ammonia were linear functions of time for 120 min. insulin (10(-6) m) increased ammonia production at 2 h by 34 +/- 5%. at the same time, gluconeogenesis, as measured by glucose production, was decreased by 16 +/- 2%. significant enhancemen ... | 1987 | 3322042 |
| cloning, characterization, and sequencing of the genes encoding the large and small subunits of the periplasmic [nife]hydrogenase of desulfovibrio gigas. | the structural genes for the large and small subunits of desulfovibrio gigas periplasmic [nife]hydrogenase were identified and isolated by immunological and oligonucleotide screening. the gene for the small subunit codes for a 266-amino-acid, 28,724-dalton polypeptide which is separated by 63 nucleotides from the large subunit gene that codes for a 560-amino-acid, 61,707-dalton polypeptide. a putative signal peptide precedes the small subunit coding region, which may direct transport of the enzy ... | 1987 | 3322743 |
| [pasteurella anatipestifer infection of water fowl]. | | 1987 | 3322779 |
| the specific gastrinase activity of membranes isolated from the human and rat gastrointestinal mucosa. | | 1987 | 3322803 |
| intraocular and extraocular retinoblastoma. | retinoblastoma, the most common primary ocular malignancy of childhood, is a tumor in which the pediatrician and pediatric oncologist can now play a much more significant role in therapy. developments in molecular biology have now made carrier testing and prenatal diagnosis feasible. in the near future, these developments should greatly augment the pediatrician's and pediatric oncologist's ability to offer accurate and appropriate genetic counseling for affected families. a practical staging sys ... | 1987 | 3323180 |
| coordinative interactions in the separation of insulin and its derivatives by high-performance liquid chromatography. | the effects of various inorganic cations and anions on the chromatographic behaviour of insulin and its derivatives have been investigated. it was shown that on an octadecylsilica column at a given eluent composition the retention of insulins depends primarily on the nature of the anions. the cations have a secondary effect, depending on their size and coordination ability. the mechanism of the effect of the ions is complex. it includes the formation of ion pairs, their influence on dissociation ... | 1987 | 3323216 |
| [quantitative integrated backscatter characteristics in the normal and infarcted canine myocardium]. | to develop ultrasonic cardiac tissue characterization, serial changes in intensity of backscattered ultrasound from the normal and infarcted myocardium were studied in vitro, with frequency-domain analysis. as an index of backscattered signal intensity, quantitative integrated backscatter [(10 x log-s2/s1)] (db), s1 and s2 = areas of power spectra of digitized (sample rate = 100 mhz, 8 bits) ultrasonic signals from a perfect reflector and good specimens) were calculated for 60 regions of the myo ... | 1986 | 3323322 |
| [endocarditis with unusual causative agents]. | in the period 1947-1985, 601 patients with infective endocarditis were seen at the university hospital zurich and the kantonsspital lucerne. streptococci, enterococci and staphylococci were the predominant causative organisms in two-thirds of all cases. in more than 25% of the patients blood cultures remained negative. in 6 patients endocarditis was caused by very rare organisms, viz. coxiella burnetii (2 cases), hemophilus parainfluenzae, corynebacterium bovis (diphtheroids), brucella melitensi ... | 1987 | 3324331 |
| [current status of the q-rickettsial endocarditis problem and the 1st case reported in bulgaria]. | a vast literary review on q-rickettsial endocarditis is presented--spread, frequency, predilection, clinical course, laboratory findings, diagnosis, treatment, prognosis. the first case of q-rickettsial endocarditis in bulgaria is reported. the case was proved by the high titre of the specific antibodies while the patient was still alive and post mortem by visualizing the causative agent in the aortic valve and by its isolation through inoculation of material from the aortic valve. the infection ... | 1987 | 3324486 |
| epidemiology (1980-1985) and nonspecific prophylaxis of q fever in the ussr (survey). | a review of the epidemiology and nonspecific prophylaxis of q fever in the ussr during 1980-1985 is presented. attention is paid of the source to infection, the seroprevalence of antibodies to coxiella burnetii in man and livestock and the possibilities of prophylaxis against q fever under conditions of mandatory registration of this disease. | 1987 | 3324565 |
| epidemiology and significance of q fever in czechoslovakia. | over the last 35 years most parts of czechoslovakia have experienced q fever. of particular note were outbreaks in factories processing imported cotton, wool and hides that had been contaminated in their countries of origin. some areas of the southern part of central slovakia became a natural focus of q fever with the dermacentor marginatus tick being the main vector of coxiellae. currently, coxiella (c.) burnetii infection appears to be dormant in the country since only sporadic cases are encou ... | 1987 | 3324566 |
| infections with coxiella burnetii in man and animals in the netherlands. | a serological survey using an indirect micro-immunofluorescence test among people considered to be at risk of contracting an infection with c. burnetii yielded 75.9% seropositives, whereas controls from three geographical regions in the netherlands showed a mean of 45.5% with considerable differences per region and sex. a comparable retrospective sample from 1968 showed 46% seropositives. sera from people aged 0 to 19 years yielded 38.3% positives (8). the preliminary data of a survey among catt ... | 1987 | 3324567 |
| q fever in the united kingdom and ireland. | in britain, q fever in man was first reported in 1949. two years later a serological survey of cattle revealed a prevalence of infection of 2.1%. in the 1970's prevalences of 4.5% and 3.1% were encountered respectively in cows that had aborted or calved normally in central england. in sheep, antibodies to c. burnetii have been detected in approximately 2.8% of sera examined for diagnostic purposes and 0.6% of sheep awaiting export. since 1975 fewer than 10 incidents of c. burnetii associated dis ... | 1987 | 3324568 |
| epidemiology and significance of q fever in the federal republic of germany. | in the federal republic of germany no large q fever epidemics (more than 200 cases) have been encountered within the last 20 years; however, q fever was prevalent throughout that period on a constant level (between 27 and 100 officially reported cases per year). besides classical pneumonic q fever, chronic forms associated with endocarditis, myocarditis and hepatitis were recently diagnosed for the first time in the federal republic of germany. the disease q fever in humans is often misdiagnosed ... | 1987 | 3324569 |
| q fever laboratory diagnostic methods used in roumania. | the methods used presently in roumania for q fever laboratory diagnosis are presented. they include coxiella (c.) burnetii isolation procedures, q fever serologic tests, and the skin test. the diagnosis by isolation procedures is performed by inoculation into guinea pigs and embryonated eggs. q fever serologic diagnostic tests are the complement fixation (cf), microagglutination (ma), and igg-igm elisa tests. results obtained by comparing the cf test with ma and elisa tests in the diagnosis of a ... | 1987 | 3324570 |
| detection of coxiella burnetii by the immunoperoxidase technique. | an immunoperoxidase method using coxiella (c.) burnetii-specific hyperimmune serum raised in rabbits, and swine anti-rabbit horseradish peroxidase conjugate was applied to visualize c. burnetii in bgm cell cultures. the technique proved to be highly specific and did not result in any unspecific background staining. the technique was applied successfully to study the organism during multiplication in vacuoles of epithelial cells and in the phagolysosomes of macrophages. preliminary results furthe ... | 1987 | 3324571 |
| q fever vaccines: present status and application in man. | of three q fever vaccine candidates available at present, i.e., phase i corpuscular untreated, soluble, and phase i corpuscular chloroform-methanol treated vaccines, the first two were tested on hundreds of subjects exposed to q fever. based on their sufficient immunogenicity (which depends also on the tests employed) and low reactogenicity (providing that subjects with previous contact with coxiella (c.) burnetii are excluded from vaccination) these two vaccines can be recommended for vaccinati ... | 1987 | 3324573 |
| q fever vaccines for animals. | the results of different workgroups and those obtained in own investigations with q fever vaccines for cattle and sheep are reviewed and discussed. in field trials coxiella (c.) burnetii vaccines of inactivated whole cells (wc) in phase (ph) i as well as wc ph ii vaccines prevented infections of cows exposed to naturally infected environments, provided they were vaccinated as noninfected calves. to monitor calves for this purpose the sensitive elisa has superseded the complement fixation test (c ... | 1987 | 3324574 |
| epidemiology and ecology of rickettsial diseases in the people's republic of china. | since 1949, information on rickettsial diseases in the people's republic of china has been virtually nonexistent in the west. this is the first comprehensive review of the ecology and epidemiology of chinese rickettsial diseases to be published outside the people's republic. at least five rickettsioses exist in china: scrub typhus, murine typhus, epidemic typhus, q fever, and one or more spotted fever-group (sfg) rickettsioses. although epidemic typhus has been controlled and scrub typhus has ab ... | 1987 | 3326129 |
| genetically engineered antibody molecules and their application. | immunoglobulin genes can be efficiently expressed following transfection into myeloma cells. using protoplast fusion, transfection frequencies greater than 10(-3) can be achieved. compatible plasmids containing two different selectible markers are used to simultaneously deliver heavy and light chain genes to the same cell. to produce molecules with differing specificities the rearranged and expressed variable regions can be cloned from the appropriate hybridoma. in some cases, variable regions f ... | 1987 | 3327412 |
| an outbreak of q fever probably due to contact with a parturient cat. | thirty-three cases (24 definite, nine probable) of q fever were diagnosed in victoria county, cape breton, nova scotia from may to august, 1985. twenty-six of the cases occurred in residents of baddeck (population 900, attack rate 2.8 percent), and 21 of the cases occurred during the month of june. there was geographic clustering of the cases: 14 of the 33 (42 percent) lived or worked in four buildings located side by side in the center of town. a case control study revealed that 25 of 29 cases ... | 1988 | 3335174 |
| lipopolysaccharide phase variation determines the complement-mediated serum susceptibility of coxiella burnetii. | phase variation of coxiella burnetii is due to variation of the lipopolysaccharide (lps), a phenomenon analogous to smooth-to-rough lps variation of gram-negative enteric bacteria. virulent enterobacteria usually have a smooth lps and resist serum killing, whereas avirulent rough lps mutants are sensitive to complement-mediated serum killing. like gram-negative enterobacteria, smooth lps phase variants of c. burnetii are virulent, whereas the rough lps variants are avirulent. we therefore studie ... | 1988 | 3335408 |
| the cpg dinucleotide and human genetic disease. | reports of single base-pair mutations within gene coding regions causing human genetic disease were collated. thirty-five per cent of mutations were found to have occurred within cpg dinucleotides. over 90% of these mutations were c----t or g----a transitions, which thus occur within coding regions at a frequency 42-fold higher than that predicted from random mutations. these findings are consistent with methylation-induced deamination of 5-methyl cytosine and suggest that methylation of dna wit ... | 1988 | 3338800 |
| q fever. a call to heighten our index of suspicion. | the prevalence of q fever infection is probably underestimated. in michigan, the first two reported human cases of q fever occurred in 1984. the case-patients lived in adjacent, rural counties and had multiple exposures to goats. we conducted a serosurvey of goat owners and a reference population to compare the prevalence of q fever antibodies in the two-county area. goat owners were almost three times more likely to be seropositive with q fever antibodies than the reference population (43% vs 1 ... | 1988 | 3341862 |
| a heat shock operon in coxiella burnetti produces a major antigen homologous to a protein in both mycobacteria and escherichia coli. | a gene library from the dna of coxiella burnetii has been constructed in the cosmid vector phc79. a particular clone, pjb196, reacted strongly with coxiella-specific antibodies elicited in a number of different species of animals. this clone produced two abundant c. burnetii-specific polypeptides, a 14-kilodalton nonimmunoreactive protein and a 62-kilodalton immunoreactive protein. sequencing identified two open reading frames, encoding polypeptides of 10.5 and 58.3 kilodaltons. the only transcr ... | 1988 | 3343219 |
| cytoskeleton-associated plectin: in situ localization, in vitro reconstitution, and binding to immobilized intermediate filament proteins. | the association and interaction of plectin (mr 300,000) with intermediate filaments and filament subunit proteins were studied. immunoelectron microscopy of whole mount cytoskeletons from various cultured cell lines (rat glioma c6, mouse balb/c 3t3, and chinese hamster ovary) and quick-frozen, deep-etched replicas of triton x-100-extracted rat embryo fibroblast cells revealed that plectin was primarily located at junction sites and branching points of intermediate filaments. these results were c ... | 1988 | 3346324 |
| d-periodic distribution of collagen type ix along cartilage fibrils. | it has recently become apparent that collagen fibrils may be composed of more than one kind of macromolecule. to explore this possibility, we developed a procedure to purify fibril fragments from 17-d embryonic chicken sternal cartilage. the fibril population obtained shows, after negative staining, a uniformity in the banding pattern and diameter similar to the fibrils in situ. pepsin digestion of this fibril preparation releases collagen types ii, ix, and xi in the proportion of 8:1:1. rotary ... | 1988 | 3346333 |
| markers of cell-mediated immunity after vaccination with an inactivated, whole-cell q fever vaccine. | a clinical trial of q fever vaccine in four south australian abattoirs showed apparently complete protection against natural infection; however, only 50%-60% of vaccinees developed complement-fixing or immunofluorescent antibody after vaccination. cell-mediated immunity to coxiella burnetii antigens, as measured by an index of lymphoproliferative responses (lsi) of peripheral blood mononuclear cells, was therefore assessed. eighty-five percent of 13 subjects with "low risk" of exposure to q feve ... | 1988 | 3346570 |
| serosurvey of coxiella burnetii infection in dairy goat herds in ontario. a comparison of two methods of enzyme-linked immunosorbent assay. | two technical variations of enzyme-linked immunosorbent assay for the detection of antibodies to coxiella burnetii were compared in this serosurvey on 20 ontario dairy goat herds. both a trichloracetic acid extract and a coctoantigen of purified coxiellas were used to sensitize the microtitration plates. technical differences related to coating ph, serum dilutions tested and interpretation of results. results agreed in 98.6% of sera examined, the differing sera were in the low titer borderline r ... | 1988 | 3349400 |
| glomerular nephropathy associated with chronic q fever. | of three patients with coxiella burnetii endocarditis, two developed focal segmental proliferative glomerulonephritis (gn), and the third developed diffuse intracapillary proliferative glomerulonephritis. in one case, a good therapeutic response was followed by partial remission of the renal alterations, but 10 months later there were clinical and histological signs of active glomerular nephropathy, suggesting that the antigenic stimulus persisted. in another case, poor evolution of the infectio ... | 1988 | 3354566 |
| serosurvey on the occurrence of coxiella burnetii in ontario cattle. | | 1988 | 3355970 |
| molecular mechanisms in basement membrane complications of diabetes. alterations in heparin, laminin, and type iv collagen association. | we report alterations in the ligand-binding properties of laminin, the major noncollagenous protein of basement membranes, resulting from nonenzymatic glycosylation in vitro. mouse laminin was incubated in vitro with 500 mm glucose, and the level of nonenzymatically glycosylated amino acids increased 2-, 6.2-, and 12-fold after incubation for 1, 3, and 12 days, respectively. ligand binding assays conducted in solution with varying concentrations of [3h]heparin and a constant amount of control or ... | 1988 | 3360214 |
| antiherpesvirus activity of 9-(4-hydroxy-3-hydroxymethylbut-1-yl) guanine (brl 39123) in animals. | the antiviral activity of 9-(4-hydroxy-3-hydroxymethylbut-1-yl)guanine (brl 39123) was assessed in several animal models of herpes simplex virus (hsv) infection. brl 39123 was as active as acyclovir (acv) when applied topically to guinea pigs with a cutaneous hsv type 1 (hsv-1) infection and was also active topically in an hsv-2 genital infection. before systemic administration to infected animals, brl 39123 and acv were administered orally and subcutaneously to mice, and the blood was assayed f ... | 1988 | 3364954 |
| glycine transport by cultured human fibroblasts. | the transport of glycine was studied in cultured human fibroblasts. the amino acid entered the cell by na+-dependent and na+-independent mechanisms. na+-independent glycine (0.1 mm) transport was less than 10% of total uptake and occurred by a mechanism formally indistinguishable from diffusion. two distinct routes contributed to na+-dependent glycine transport. the first route was identified with system a because it was inhibited by meaib and underwent adaptive regulation. the second route was ... | 1988 | 3365244 |
| duplication 17q mosaicism: an infant with features of ellis-van creveld syndrome. | we describe an infant with multiple dysmorphic features who is mosaic for duplication 17q21.1----qter, owing to a direct tandem duplication. he is the first case with mosaicism for a 17q duplication to be reported. his features are strikingly suggestive of ellis-van creveld syndrome. | 1988 | 3367352 |
| aids and adolescents: school health education must begin now. | teenagers must be educated about aids. because thousands of teenagers are at risk because of their behaviors, target groups for special programs should include those who are drug users, gay and bisexual, homeless, or into solicitation and prostitution. educational material should be developed that includes a culturally sensitive approach for minority teenagers. certain areas with high prevalence rates of aids need special attention because they pose greater risks for teenagers, such as housto ... | 1988 | 3367641 |
| incidence of q fever among cattle, sheep and goats in the upper nile province in southern sudan. | | 1988 | 3369066 |
| [effect of prolactin on the enzymatic activity of thyroid follicular cells during stress due to surgery and subsequent subcutaneous inflammation]. | in order to reveal prolactin (prl) effect on the enzymatic activity of the thyroid follicular cells at a subcutaneous inflammation, 2 series of experiments have been performed on 100 non-inbred male rats with body mass of 150-170 g. in order to produce the inflammation, under ether narcosis celloidin globules are introduced subcutaneously in the abdominal wall. the test animals are given 0.125 mg of "lactin" (the soviet preparation of bovine prl) in 0.5 ml of isotonic solution once every day, be ... | 1988 | 3369954 |
| effect of chronic naltrexone administration and its withdrawal on the regional activity of neurons that contain norepinephrine, dopamine and serotonin. | a method is described that permits the simultaneous quantitation of norepinephrine (ne), dopamine (da) serotonin (5-ht) and their respective major metabolites, 3-methoxy-4-hydroxy phenylglycol (mhpg), 3-methoxytyramine (3-mt), dihydroxyphenyl acetic acid (dopac) and 5-hydroxyindole acetic acid (5-hiaa) in discrete brain regions. the ratio of mhpg/ne, dopac/da and 5-hiaa/5-ht was used to assess the effects of the chronic administration of the narcotic antagonist, naltrexone, and its withdrawal on ... | 1988 | 3370455 |
| lack of effect of adrenal denervation on analgesia elicited by continuous and intermittent cold water swim in the rat. | the present experiment was conducted to determine if continuous and/or intermittent cold water swim analgesia is dependent upon a factor released from the adrenal medullae. male sprague-dawley rats (300-350 g) underwent either bilateral adrenal denervation or sham surgery and two weeks later were exposed to continuous (3.5 m) or intermittent (10 s in, 10 s out for 6 min) swim in water at 4 degrees c. adrenal denervation failed to affect either intermittent or continuous cold water swim analgesia ... | 1988 | 3370473 |
| fatal respiratory disease in nilgiri tahr: possibly malignant catarrhal fever. | nilgiri tahr (hemitragus hylocrius) are native to india and are a rare zoo exhibit. this report describes an acute respiratory disease in tahr that caused the death of 15 of 16 animals in an extensive exhibit of about 35 acres where they were housed together with a variety of other exotic species of ruminants. the deaths occurred in two separate outbreaks and were associated with losses from malignant catarrhal fever in other ruminants in the exhibit. the most prominent clinical sign was severe ... | 1988 | 3370557 |
| stabilization of osteochondral fragments using limited placement of cyanoacrylate in rabbits. | the higher homologues of the alkyl-2-cyanoacrylates appear to possess properties useful for an osseous adhesive. considering currently available bonding strengths, the most reasonable potential application of an osseous adhesive would appear to involve the stabilization of relatively small osteochondral fragments. if used as a means of temporary internal fixation, such an adhesive would need to be used in limited amounts in limited areas to allow for normal healing processes to occur between are ... | 1988 | 3370879 |
| cloning, nucleotide sequence, and taxonomic implications of the flagellin gene of roseburia cecicola. | the gene coding for the flagellin protein of roseburia cecicola, an oxygen-intolerant, gram-negative, anaerobic bacterium indigenous to the murine cecum, has been cloned and sequenced. nh2-terminal amino acid sequence data from the flagellin protein were used as a basis for the synthesis of two mixed-sequence deoxyoligonucleotides. the oligonucleotides were used to identify and clone the flagellin structural gene. dna sequence analysis of m13mp8 and mp9 subclones revealed a protein with a length ... | 1988 | 3372477 |
| defect in cholesteryl ester transport in serum of patients with uremia receiving maintenance hemodialysis: increased inhibitor activity for cholesteryl ester transfer. | the rate of cholesterol transfer (rct) from high-density lipoproteins (hdls) to very low-density lipoproteins (vldls) and low-density lipoproteins (ldls) was previously found to be lower in the serum of patients with uremia receiving maintenance hemodialysis than in the serum of control subjects, and the defect was associated with the serum density greater than 1.063 gm/ml fraction (hsia et al. j lab clin med 1985;106:53). the present study shows that cholesterol transfer from hdls to vldls and ... | 1988 | 3373112 |
| [effect of acute and chronic administration of melatonin on the temporal dynamics of apomorphine-induced stereotypy]. | melatonin (1-20 mg/kg) weakened in a dose-dependent way the intensity degree of apomorphine-induced stereotypy in rats. according to the spectral analysis data, this is followed by an increase of the number of waves with a short (2-3 per min) period in the temporal structure of stereotypy recorded every one minute. after administration of melatonin (1 mg/kg), the effect of apomorphine changed more in the afternoon (1-3 p.m.) than in the morning (6-8 a.m.) or the evening (6-8 p.m.) hours. the act ... | 1988 | 3378603 |
| q fever endocarditis: relapse five years after successful valve replacement for a first unrecognized episode. | a 59-yr-old man presented with mitral endocarditis and negative blood cultures. antibodies to phase 2 and phase 1 antigens of coxiella burneti were detected and a diagnosis of q fever endocarditis was made. five years earlier, this patient had been successfully treated by aortic valve replacement for a first episode of endocarditis with negative blood cultures. giemsa and machiavello stains of the native aortic valve were made retrospectively and showed coccobacilli highly suggestive of coxiella ... | 1988 | 3379100 |
| quiescent q fever endocarditis exacerbated by cardiac surgery and corticosteroid therapy. | q fever endocarditis occurs in up to 11% of patients infected by coxiella burnetti. major clues for the diagnosis are culture-negative endocarditis, hepatic involvement, rash, and thrombocytopenia. characteristically, the diagnosis is delayed. in our patient, q fever endocarditis occurred without previously recorded signs of infection. fever, rash, and hepatic involvement all occurred following aortic valve replacement. the histologic picture of the excised valve was consistent with endocarditis ... | 1988 | 3382299 |
| [metabolism of guanidinoacetic acid and creatine in uremia]. | | 1988 | 3386016 |
| ischaemia-induced changes in the phosphorylase activity in different parts of the cardiac conduction system. | the phosphorylase specific activity was lowered in total ischaemia in all examined parts of the myocardium and the conduction system of the heart. sixty-minute ischaemia had a considerable effect on glycogen degradation in right bundle branch and in the working myocardium, however, its glycogenolytic effect on the av-node and penetrating bundle of his was insignificant. phosphorylase "a" evaluated as a percentage of the total phosphorylase activity decreased in the working myocardium and increas ... | 1988 | 3391039 |
| exposure to parturient cats: a risk factor for acquisition of q fever in maritime canada. | over a 34-mo period we studied 51 patients with q fever and 102 control subjects (with various lower-respiratory-tract infections) who were matched for age, sex, and time of onset of infection. by univariate analysis (not adjusted for multiple comparisons), cases differed significantly from controls in the following activities: working on a farm; slaughtering or dressing animals; and contact with cats, cattle, and sheep. the strongest association was with exposure to stillborn kittens--11 of 51 ... | 1988 | 3392409 |
| poker players' pneumonia. an urban outbreak of q fever following exposure to a parturient cat. | | 1988 | 3393197 |
| an ultrastructural study of the development of babesia occultans in the salivary glands of adult hyalomma marginatum rufipes. | the development of babesia occultans in the salivary glands of adult hyalomma marginatum rufipes was studied with the electron microscope. sporogony involved a process of multiple fission in which sporozoites formed from the periphery of a polymorphous sporont. different stages of development were found concurrently in individual acini as well as within individual acinar cells. mature sporozoites were found on day 3 post-tick attachment and measures 3.0-3.5 x 1.5 micron. the apical complex consi ... | 1988 | 3393374 |
| chronic q fever and severe thrombocytopenia in a pregnant woman. | | 1988 | 3400702 |
| serological reaction to legionella pneumophila group 4 in a patient with q fever. | | 1988 | 3404000 |
| the orienting response as an index of stimulus associability in rats. | the strength of the orienting response (or) to a light and its associability was studied in three experiments. in experiment 1, three groups of rats received serial conditioning in which the light served as the first element of a serial compound. for group diff the light was followed by a conditioned stimulus (cs) of 10-s duration on some trials and by a different cs of 30-s duration on others. for the other groups, the light was also followed by different stimuli, but these had equivalent durat ... | 1988 | 3404083 |
| conversion of angiotensin i to angiotensin ii by cathepsin a isoenzymes of porcine kidney. | we have reported the existence of a carboxypeptidase in a human renal extract that converts angiotensin i (ai) to angiotensin ii (aii) in two steps with des-leu-ai (dl-ai) being formed as an intermediate. since this carboxypeptidase had properties similar to cathepsin a, the ability of cathepsin a to metabolize ai was studied. cathepsin a was purified from hog kidney with enzyme activity being monitored using both benzyloxycarbonyl-glutamyl-tyrosine (zgt) and ai as substrates. the procedure sepa ... | 1988 | 3408489 |
| hepatic fibrin-ring granulomas in visceral leishmaniasis. | hepatic fibrin-ring granulomas and leishmania parasites were found in the liver biopsy specimens of 3 patients with prolonged fever and hepatosplenomegaly. it was recognition of the leishmanias in the liver biopsy specimen that prompted the diagnosis in all cases. there was no evidence of q fever, hodgkin's disease, cytomegalovirus hepatitis, or allopurinol treatment, which are the recognized causes of hepatic fibrin-ring granulomas. this report extends the range of etiologies of hepatic fibrin- ... | 1988 | 3410227 |
| tuberculosis in five basset hounds. | five basset hounds (2 females and 3 males) under the age of 5 years, acquired systemic tuberculosis. we suspected tuberculosis in one dog, because it had histologic lesions similar to those in 4 dogs in which bacteria were identified as mycobacterium avium complex. a review of canine tuberculosis revealed a similar diagnosis in a basset hound. the association of this infection in basset hounds suggests an inherited immunologic defect. results of our survey suggest that the defect might exist in ... | 1988 | 3410774 |
| the regionalization of laboratory services at the university of utah medical center. associated regional and university pathologists inc (arup). | we feel that now, four years out, we have achieved the interim goals that we set for ourselves. there are some key aspects that we would do differently if we were now starting over, and we have provided that information to those seeking to begin similar off-site ventures who have sought our advice. there is an interesting constellation of factors that must be present for such an operation to succeed, and the individual components of that constellation will differ by institution and location. the ... | 1988 | 3415450 |
| optic disc, cup and neuroretinal rim size, configuration and correlations in normal eyes. | four hundred and fifty-seven unselected normal human optic nerve heads of 319 subjects (163 men, 156 women, mean age 42.7 +/- 19.6 years) were evaluated by magnification-corrected morphometry of optic disc photographs. mean optic disc surface measured 2.69 +/- 0.70 mm2 (0.80-5.54 mm2), mean diameter horizontally 1.76 +/- 0.31 mm (0.91-2.61 mm), and vertically 1.92 +/- 0.29 mm (0.96-2.91 mm). the form was slightly vertically oval. optic cup area averaged 0.72 +/- 0.70 (0.00-3.41 mm2), mean horizo ... | 1988 | 3417404 |
| abortion in black belly barbados sheep in fiji caused by coxiella burnetii. | | 1988 | 3421889 |
| [seropositivity in occupationally exposed persons to toxoplasmosis, q-fever and chlamydiosis]. | | 1987 | 3427492 |
| release of neuronotrophic factor from rabbit corneal epithelium during wound healing and nerve regeneration. | epithelial neuronotropic factor (enf) is secreted by cultured epithelial cells of rabbit cornea and conjunctiva, and is active in promoting survival and inducing neurite outgrowth of cultured trigeminal neurons. this study evaluated the relation of enf to corneal nerve regeneration utilizing a model of heptanol-induced epithelial wounding. the organ culture technique was used to collect enf from the intact corneal epithelium, and a neuronal bioassay was utilized to quantify enf. the results reve ... | 1987 | 3428389 |
| identification of a peanut agglutinin-binding protein from human retina. | with the use of an antiserum raised to peanut agglutinin (pna)-binding protein(s) from human retinas, we have identified a glycoprotein that has the same subunit molecular weight (135,000) as human interphotoreceptor retinol-binding protein (irbp) on human retinal protein transblots, and, like irbp, is localized in the interphotoreceptor matrix surrounding both rods and cones. we show that this pna-binding protein is not irbp by comparing the binding patterns of antiserum to the pna-binding prot ... | 1987 | 3428393 |
| serological and bacteriological survey of haemophilus pleuropneumoniae serovar 5. | | 1987 | 3430925 |
| [q fever in uruguay]. | | 1987 | 3432922 |
| epidemiology and significance of q fever in hungary. | in hungary, coxiella (c.) burnetii infections were diagnosed for the first time in 1956 in a few dairy and sheep farms. q fever associated with abortion was first demonstrated in 1983 during laboratory diagnostic examination of fetuses and placentae. from case history data and on-the-spot investigations it is clear that in cattle stocks abortion caused by c. burnetii occurs sporadically, whereas in sheep flocks numerous abortions may take place within a short time. losses caused by q fever on th ... | 1987 | 3434013 |
| epidemiology of q fever in italy and in other mediterranean countries. | the history of q fever in italy may be divided into three periods: epidemic in character after the second world war, endemic occurrence from 1960 to 1980, and sporadic occurrence at present. clinical symptoms are unspecific, and diagnosis must be confirmed by serology and isolation of the causative agent. the reported incidence is consequently underestimated. results are reported of a seroepidemiologic survey in animals and humans in the italian region and western sicily. in the mediterranean ar ... | 1987 | 3434014 |
| q fever in france. | in france q fever is a notifiable disease, but information could only be obtained from fragmental inquiries. routine serologic examination of 75 901 sera during the years 1982-85 showed 430 q fever positive sera (mean rate 0.57%). our laboratory could confirm 135 cases of clinical q fever. in the region of dijon, 4.4% of the country population was seropositive, but among breeders 25% were positive. 36 persons (8%) among 245 employees of the departmental veterinary services were seropositive, som ... | 1987 | 3434015 |
| serodiagnosis of q fever by enzyme-linked immunosorbent assay (elisa). | indirect enzyme-linked immunosorbent assays (elisas) specific for igg and igm antibodies against coxiella burnetii were applied to test 208 serum samples collected within 1983 to 1986 from 128 individuals suspected of having q fever, and from 1611 serum samples of normal blood donors. among them were 2 patients with q fever endocarditis, one patient with myocarditis, one patient with chronic hepatitis, 3 patients with pneumonia, one woman who had aborted a monstrous child, 38 state veterinarians ... | 1987 | 3434017 |
| re: prophylaxis of gram-negative and gram-positive infections in rodents with three intravenous immunoglobulins and therapy of experimental polymicrobial burn wound sepsis with pseudomonas immunoglobulin and ciprofloxacin. | | 1987 | 3436681 |
| [differential pathomorphological diagnosis of q fever and chlamydia and toxoplasma abortions in sheep]. | morphologic differential diagnostic investigations were carried out with a total of 134 aborted sheep fetuses. the studies were coupled by the examination of 665 blood serum samples from sheep that were pregnant or had miscarried. parallel bacteriologic studies were also carried out. positive serologic results were obtained in 43.74 per cent of the cases (toxoplasmosis), 20.2% (chlamydial infection), and 14.4% (q-fever). gross lesions were found in 64 fetuses (toxoplasmosis), 18 (q-fever), and 3 ... | 1987 | 3439024 |
| coagulase-negative staphylococci resistant to beta-lactam antibiotics in vivo produce penicillin-binding protein 2a. | strains of coagulase-negative staphylococci were tested for in vivo resistance in a rabbit model of prophylaxis of endocarditis. regimens of nafcillin, cefazolin, cefamandole, and vancomycin were compared for efficacy in the prevention of infection caused by two methicillin-resistant strains and a susceptible strain. for the two resistant strains, vancomycin was the most effective drug tested. all regimens were effective against the susceptible strain. the two strains for which prophylaxis with ... | 1987 | 3439802 |
| lipid a and related compounds. xiv. a new synthesis of lipid y, the reducing sugar moiety of salmonella- and proteus-type lipid a. | | 1987 | 3442881 |
| [toxicological evaluation of water-soluble polymer lubricant-cooling liquids]. | | 1987 | 3443326 |
| research on a possible q-fever infection in humans and animals on the cape verde islands (santa cruz/santiago, west africa). | we tested 243 domestic-animal serum samples and 316 human serum samples from the st. cruz region on santiago island for antibodies against coxiella burneti. 145 (59.6%) of the animal sera and 41.7% of the human sera tested positive. the samples were obtained after an epidemic among the human population with fever and symptoms typical of q-fever. | 1987 | 3443745 |
| serological investigations in nigeria for anthropozoonoses in human sera: brucellosis, echinococcosis, toxoplasmosis, chlamydial diseases, listeriosis, rickettsiosis (coxiella burneti and rickettsia conori). | 176 blood sera taken from patients in the hospital of minna and abeokuta (nigeria) were examined for anthropozoonoses. the following positive reactions could be found: brucella abortus 9%, brucella melitensis 11.7%, echinococcosis 0.53%, toxoplasmosis 79.2% (cf-test 6.9%), chlamydial diseases 45%, listeriosis 28.7% (typ 1h) and 19.7% (typ 4bh), rickettsiosis--rickettsia conori 18.6% and coxiella burneti 63.3%. | 1987 | 3443751 |
| the incidence of coxiella burnetii antibodies in cattle in the transvaal. | with the use of the complement fixation test, 8,900 cattle were tested for antibodies to coxiella burnetii. these were randomly selected from 178 different farms in 37 districts in the transvaal. the percentage of cattle in the sample with positive antibody titres was equal to 7.78%, with a standard error of 0.28%. because of the large size of the sample, asymptotic normality can be relied upon and the population confidence interval calculated. this was found to be greater than or = 0.07 and les ... | 1987 | 3444611 |