| characterization of impermeability variants of pseudomonas aeruginosa isolated during unsuccessful therapy of experimental endocarditis. | we characterized five amikacin-resistant variants of pseudomonas aeruginosa isolated from aortic valve vegetations during unsuccessful therapy of experimental endocarditis. these organisms were cross resistant to other aminoglycosides. no aminoglycoside-modifying enzymes were produced by these strains. however, all five variants demonstrated significant defects in permeability and intracellular uptake of [3h]amikacin when compared with the amikacin-susceptible parental strain (0 to 26% of that o ... | 1987 | 3105442 |
| characterization of impermeability variants of pseudomonas aeruginosa isolated during unsuccessful therapy of experimental endocarditis. | we characterized five amikacin-resistant variants of pseudomonas aeruginosa isolated from aortic valve vegetations during unsuccessful therapy of experimental endocarditis. these organisms were cross resistant to other aminoglycosides. no aminoglycoside-modifying enzymes were produced by these strains. however, all five variants demonstrated significant defects in permeability and intracellular uptake of [3h]amikacin when compared with the amikacin-susceptible parental strain (0 to 26% of that o ... | 1987 | 3105442 |
| salt tolerance of lactose-grown vibrio parahaemolyticus carrying escherichia coli lac genes. | lac- strains of vibrio parahaemolyticus were converted to lac+ on receiving a hybrid plasmid containing the lactose utilization genes of escherichia coli k-12. a v. parahaemolyticus strain containing this hybrid plasmid exhibited optimal growth rates on glucose and other carbon sources in the presence of 0.2 to 0.4 m nacl. growth of the same strain on lactose was inhibited at similar concentrations of nacl. the altered growth rate responses in lactose medium appeared to be attributable to effect ... | 1987 | 3105458 |
| [antimicrobial susceptibility of vibrio parahaemolyticus and vibrio alginolyticus isolated from human feces and foods]. | | 1987 | 3106535 |
| biosynthesis and processing of platelet gpiib-iiia in human megakaryocytes. | platelet membrane glycoprotein iib-iiia forms a calcium-dependent heterodimer and constitutes the fibrinogen receptor on stimulated platelets. gpiib is a two-chain protein containing disulfide-linked alpha and beta subunits. gpiiia is a single chain protein. these proteins are synthesized in the bone marrow by megakaryocytes, but the study of their synthesis has been hampered by the difficulty in obtaining enriched population of megakaryocytes in large numbers. to examine the biosynthesis and pr ... | 1987 | 3108266 |
| differences between saccharomyces cerevisiae and bacillus subtilis in secretion of human lysozyme. | saccharomyces cerevisiae secreted human lysozyme in the medium as an active form when the signal peptides of chicken lysozyme and a chicken lysozyme-aspergillus awamori glucoamylase hybrid were used, whereas it did not synthesize any human lysozyme protein by using the signal peptide of a. awamori glucoamylase. the secreted lysozyme was easily purified and crystallized. on the other hand, bacillus subtilis secreted an inactive human lysozyme, which seemed to have incorrect disulfide bonds, with ... | 1987 | 3109419 |
| stimulation by hyphopichia burtonii and bacillus amyloliquefaciens of aflatoxin production by aspergillus flavus in irradiated maize and rice grains. | aspergillus flavus was grown on maize and rice extract agars and on irradiated viable cracked maize and rice grains, either in pure culture or in dual culture with wild strains of either hyphopichia burtonii or bacillus amyloliquefaciens. aflatoxin production by a. flavus and its growth and interactions with the other microorganisms were studied at three water activities (aw) (0.98, 0.95, and 0.90) and two temperatures (25 and 16 degrees c). both h. burtonii and b. amyloliquefaciens markedly sti ... | 1987 | 3111368 |
| [drug-resistance and transferable r plasmids in vibrio cholerae o-1 and non o-1, v. fluvialis and v. parahaemolyticus recently isolated from human sources]. | | 1987 | 3112272 |
| distribution of indicator bacteria and vibrio parahaemolyticus in sewage-polluted intertidal sediments. | the impact of a sewage point source on the bacterial densities in an intertidal mud flat in boston harbor, mass., was investigated. the area, savin hill cove, acts as a receiving basin for a combined storm and sewage outlet (cso). preliminary examination of sediments and overlying water at high tide demonstrated that fecal coliforms were present in sediments at abundances 2 to 4 orders of magnitude higher than in the overlying water column. the following bacterial counts were determined from sed ... | 1987 | 3116932 |
| purification and characterization of a hemolysin produced by a clinical isolate of kanagawa phenomenon-negative vibrio parahaemolyticus and related to the thermostable direct hemolysin. | a clinical isolate (strain 4037) of kanagawa phenomenon-negative vibrio parahaemolyticus was studied. although the strain was judged to be kanagawa phenomenon-negative by various conventional tests, it produced a new hemolysin (named vp-trh, for thermostable direct hemolysin [vp-tdh]-related hemolysin) that was related to the vp-tdh produced by ordinary kanagawa phenomenon-positive v. parahaemolyticus. vp-trh was purified by ammonium sulfate fractionation and successive column chromatographies o ... | 1988 | 3126151 |
| decreased locomotor activity produced by repeated, but not single, administration of murine-recombinant interferon-gamma in mice. | a single treatment with murine-recombinant interferon-gamma (murifn-gamma; 30 micrograms/mouse), whether evaluated immediately after, or four hrs after, intraperitoneal injection, does not alter open field activity levels. on the other hand, repeated murifn-gamma administration (30 micrograms/mouse/day for 5 days) results in decreased spontaneous locomotor activity and an increase in body weight. | 1988 | 3126371 |
| [vibrio parahaemolyticus infectious disease caused by kanagawa phenomenon-negative o3:k6 originated from maldives]. | | 1987 | 3127502 |
| serum albumin adducts in the molecular epidemiology of aflatoxin carcinogenesis: correlation with aflatoxin b1 intake and urinary excretion of aflatoxin m1. | aflatoxin-serum albumin adducts in the blood of 42 residents of guangxi province, people's republic of china, were determined and compared with intake of aflatoxin b1 (afb1) and excretion of aflatoxin m1 (afm1) in urine. blood specimens were obtained during the same period that urine was collected and that diet was sampled. serum albumin was isolated from blood by affinity chromatography on reactive blue 2-sepharose and subjected to enzymatic proteolysis using pronase. immunoreactive products we ... | 1988 | 3133131 |
| prostacyclin biosynthesis and phospholipase activity in hypoxic rat myocardium. | phospholipid metabolism was studied in rat myocardial slices that were incubated under normoxia or hypoxia for up to 24 hours. phospholipid degradation was prominent in hypoxic myocardium, particularly phosphatidylcholine, which markedly decreased after 24 hours of hypoxia. in contrast, lysophosphatidylcholine increased. the mechanism of phospholipid degradation in hypoxic myocardium was studied. the highest activity for phospholipase a2 among subcellular fractions was found in microsomal fracti ... | 1988 | 3133132 |
| bone mineral metabolism in experimental diabetes mellitus: osteocalcin as a measure of bone remodeling. | the etiology of diabetic osteopenia has not been established. the value of serum osteocalcin (bgp) as a marker of the bone abnormalities and the possible role of the polyol pathway in diabetic osteopenia were investigated. three groups of rats were studied over 7 weeks: group d (n = 12), rats with streptozotocin (55 mg/kg)-induced diabetes given saline by gavage; group ds (n = 12), rats with streptozotocin-induced diabetes given the aldose reductase inhibitor sorbinil (25 mg/kg) daily by gavage; ... | 1988 | 3133194 |
| on the diagnostic significance of cft in respiratory-syncytial virus infection in children over one year old. | | 1987 | 3133290 |
| hiv seroprevalence in migrant and seasonal farmworkers--north carolina, 1987. | | 1988 | 3136308 |
| vibrio parahemolyticus-associated diarrhea in spain. | | 1988 | 3137059 |
| development of a highly sensitive bead-elisa to detect bacterial protein toxins. | a highly sensitive sandwich enzyme-linked immunosorbent assay to detect bacterial toxins was developed. fab' of anti-toxin igg was conjugated with horseradish peroxidase by the maleimide method and tetramethylbenzidine was used as substrate. as the solid phase, a 6.5 mm diameter polystyrene bead was used and this was coated with the anti-toxin igg. the entire assay could be completed within 3.5 hr. the sensitivity of this bead-elisa was found to be quite high with various bacterial toxins: less ... | 1988 | 3143897 |
| poor lifestyle modification among intravenous drug users assisted at a sexually transmitted disease clinic for hiv infection diagnosis, madrid, 1987. | | 1988 | 3146273 |
| prevention of respiratory distress syndrome. | rds continues to be a major problem for premature infants despite a better understanding of its pathophysiology and of ways to try to prevent it. to date, prenatal administration of glucocorticoids has been the most widely used method of accelerating fetal lung development. however, several limitations of this therapy have prompted the search for alternative approaches. most efforts have focused on the potential use of combined hormonal therapy with glucocorticoids and either thyroid hormones or ... | 1988 | 3146813 |
| isolation of the early phase of chylomicron formation in intestinal epithelial cells of rats. | lipid is first observed electron microscopically in the rough endoplasmic reticulum of intestinal epithelial cells during active lipid absorption. we have been able to isolate this subcellular fraction by using discontinuous sucrose gradients of 0.25/0.86/1.11 m sucrose. a preliminary low speed centrifugation of mucosal homogenate removed the heavier subcellular organelles. the resulting supernatant was centrifuged at 5.25 x 10(6) x g.min. the pellet from this centrifugation was placed on top of ... | 1988 | 3147719 |
| [effects of intracerebroventricularly injected cholecystokinin octapeptide on the electric activities of pain-reacted neurons in nucleus parafascicularis of thalamus in rats]. | | 1988 | 3151154 |
| the iridovirus frog virus 3: a model for trans-acting proteins. | the amphibian iridovirus, frog virus 3 (fv3), produces at least two trans-activating proteins that stimulate the expression of viral genes. one of these proteins induces transcription from the promoter of an immediate-early fv3 gene, whereas the other induces transcription from exogenously methylated dna. these proteins may serve as a model for both viral and cellular trans-acting factors. | 1986 | 3153144 |
| effects of acetyl strophanthidin on duration of atrial fibrillation in the neurally-intact and blockaded dog. | although the inotropic and dromotropic effects of cardiac glycosides in atrial fibrillation (af) are well recognized, their action on af itself is not clear. accordingly, to determine whether cardiac glycosides prolong af, the duration of electrically induced af, atrioventricular conduction, and left ventricular function were assessed for 30 minutes before and for 30 minutes following intravenous administration of acetyl strophanthidin (as), 20 micrograms/kg, in neurally intact, beta-blocked, an ... | 1988 | 3154634 |
| non-immune igg f(ab')2 binding to group c and g streptococci is mediated by structures on gamma chains. | the present investigation was designed to determine whether the heavy or the light immunoglobulin chain is involved in the non-immune binding of igg f(ab')2 fragments to specific surface receptors on human group c and g streptococci. purified human polyclonal igg was mildly reduced with dithiothreitol and alkylated with iodoacetamide. light (l) and heavy (h) chains were separated. intact igg and purified l and h chains of polyclonal immunoglobulin g were tested in an inhibition assay for non-imm ... | 1985 | 3156400 |
| direct selection of mutations reducing transcription or translation of the reca gene of escherichia coli with a reca-lacz protein fusion. | when a reca-lacz protein fusion was cloned into phage lambda, the resulting transducing phage grew normally on wild-type escherichia coli, but its growth was severely inhibited in lexa(def) mutant strains that express reca constitutively at high levels. mutants of the transducing phage that grew on the lexa(def) strains were isolated and were found to affect production of the reca-beta-galactosidase hybrid protein. most mutants, including a number of nonsense mutants, were phenotypically lacz-. ... | 1985 | 3160689 |
| structural and functional studies of the effects of sympathetic nerve stimulation on rabbit submandibular salivary glands. | continuous sympathetic stimulation at 8-10 hz caused intense vasoconstriction in the gland, so stimulation was generally given in an interrupted pattern to minimize this detrimental effect on secretion. only a small increase in fluid secretion occurred; it became thick and tended to block the cannula; therefore in later experiments the main duct was not cannulated. after sympathetic stimulation there was substantial degranulation of acinar cells. however, as this was accompanied by little moveme ... | 1988 | 3165262 |
| characterization of muscarinic receptor subtypes on human peripheral lung. | to better define the role of muscarinic receptors in lung responses and airway diseases, we characterized the binding of the m1-specific antagonist, [3h]pirenzepine (pz), and the nonspecific (m1- and m2-) antagonist, [3h]quinuclidinyl benzilate (qnb), to human peripheral lung tissue. data obtained from 15 different lung specimens showed that the radioligands bound to single high-affinity sites with dissociation constant (kd) values ranging from 1 to 9 nm for [3h]pz and 0.03 to 0.46 nm for [3h]qn ... | 1988 | 3170410 |
| survival of vibrio parahaemolyticus in two gastropod molluscs, clithon retropictus and nerita albicilla. | | 1988 | 3172602 |
| isolation from a coastal fish of vibrio hollisae capable of producing a hemolysin similar to the thermostable direct hemolysin of vibrio parahaemolyticus. | a vibrio isolated from the intestine of a coastal fish was identified as vibrio hollisae by its biochemical characteristics. the isolate reacted with the gene probe for the thermostable direct hemolysin of vibrio parahaemolyticus. the hemolysin produced by the isolate from the fish had traits identical to those of the thermostable direct hemolysin-like hemolysin produced by a clinical strain of v. hollisae. | 1988 | 3178217 |
| temporal relationship of vibrio parahaemolyticus in patients and the environment. | we prospectively compared the occurrence of vibrio parahaemolyticus in patients and the environment in the pacific northwest. inpatient and outpatient stool and wound specimens and water samples from 10 estuarine sites were cultured for v. parahaemolyticus over a period of 3 years. v. parahaemolyticus infections were detected in 13 patients (8 with gastroenteritis; 5 with wound infections), and all of the infections were found in outpatients in physicians' offices. ten of the infections were loc ... | 1988 | 3183022 |
| sodium butyrate increases glucose transporter expression in llc-pk1 cells. | the effect of sodium butyrate on the expression of the facilitated glucose transporter (gt) was investigated in the pig kidney cell line llc-pk1. when cells were treated with butyrate, gt mrna expression was remarkably enhanced with a maximal effect at 5 mm. levels of gt mrna were increased at 1 day after butyrate treatment and continued to increase for at least 4 days; however, acetate and propionate did not affect gt mrna levels significantly. the induction of gt mrna by butyrate was accompani ... | 1988 | 3186708 |
| hepatotoxicity of cyclophosphamide in man: pharmacokinetic analysis. | of 44 patients with neoplasia treated with cyclophosphamide (cpa), 19 (43%) had a transient elevation of serum levels of aminotransferases. in these patients, regardless of the combination chemotherapy regimen given, the incidence of liver dysfunction was 33%, when the total cpa dose was less than 400 mg/m2 (group a) and 73% with higher doses (group b). prior to the initiation of cpa treatment, pharmacokinetics of cpa were investigated in 15 patients. plasma aucs of cpa and phosphoramide mustard ... | 1988 | 3187190 |
| differential modulation of host cell and hiv gene expression by combinations of avarol and azt in vitro. | human immunodeficiency virus type 1 (hiv-1) gene expression is modulated by some virus-encoded proteins, possibly acting at multiple levels of control, which are also known to be involved in the regulation of gene expression in uninfected cells (transcriptional, post-transcriptional, nucleocytoplasmic transport, and translational control). two anti-hiv-1 drugs, avarol and 3'-azido-3'-deoxythymidine, which inhibit viral replication by differential mechanisms, were used to study the role of cytopl ... | 1988 | 3190740 |
| chicken liver basic fatty acid-binding protein (pi = 9.0). purification, crystallization and preliminary x-ray data. | chicken liver basic fatty acid-binding protein (pi = 9.0) has been purified with a high yield by a modification of a method originally applied to rat liver. the final product is highly homogeneous and can be used to grow crystals that belong to two different space groups. the crystals are either tetragonal, space group p4(2)2(1)2 with a = b = 60.2 a and c = 138.1 a or orthorhombic, space group p2(1)2(1)2(1) with a = 60.7 a, b = 40.1 a and c = 66.7 a. the second form appears to be more suitable f ... | 1988 | 3191992 |
| activities and properties of putrescine-biosynthetic enzymes in vibrio parahaemolyticus. | the biosynthetic pathways for putrescine (put) in vibrio parahaemolyticus were delineated by measuring activities of the enzymes which would be involved in its biosynthesis. experiments with labeled arginine and ornithine revealed that both of these amino acids were converted into put by intact cells. the activities of three enzymes, arginine decarboxylase (adc), ornithine decarboxylase (odc), and agmatine ureohydrolase (auh), were detected in cell extracts. adc and odc of v. parahaemolyticus we ... | 1988 | 3193911 |
| the disposition of [2,3-14c]-methyl and [2,3-14c]-2-ethylhexyl acrylate in male wistar albino rats. | the disposition of methyl [2,3-14c]-acrylate (ma) and 2-ethylhexyl [2,3-14c]-acrylate (eha) following intraperitoneal and oral administration to rats has been studied. the 14c found in the tissues was mainly associated with liver, kidneys and lungs. loss of 14c from these tissues occurred fairly rapidly, excluding the rats given eha intraperitoneally. most of the administered acrylates underwent rapid metabolism and excretion with expired air (more than 50% of the dose and urine (10-50% of the d ... | 1988 | 3196152 |
| [food poisoning caused by halophilic vibrio parahaemolyticus]. | | 1988 | 3212540 |
| coccidiocidal efficacy of a single treatment of toltrazuril in naturally infected lambs. | | 1988 | 3212906 |
| [demonstration of new k-antigens in strains of vibrio parahaemolyticus isolated from patients with traveller's diarrhea]. | | 1988 | 3216491 |
| effect of aminooxyacetic acid on the release of preloaded [3h]gaba and radioactive metabolites from slices of developing mouse brain. | the release of [3h]gamma-aminobutyric acid (gaba) and its radioactive metabolites from slices of the cerebral cortex, cerebellum, striatum and brain stem of developing and adult mice was studied. the slices were incubated and superfused in the absence and presence of the gaba aminotransferase (gaba-t) inhibitor aminooxyacetic acid (aoaa). exposure to 100 microm aoaa totally inhibited gaba-t and all radioactivity released from slices was in authentic gaba. in studies on developing brain the 10-mi ... | 1988 | 3216949 |
| toxin profiles of vibrio cholerae non-o1 from environmental sources in calcutta, india. | a collection of vibrio cholerae non-o1 isolated from the aquatic environs of calcutta, a cholera-hyperendemic area, were examined for the production of cholera toxin (ct), shiga-like toxins (vero toxins), heat-stable enterotoxin, and hemolysins. two (0.5%) v. cholerae non-o1 isolates produced ct. the dna from both these isolates also hybridized with a dna probe containing sequences encoding the a subunit of ct. none of the strains produced shiga-like toxins or heat-stable enterotoxin. hemolytic ... | 1988 | 3223774 |
| chlamydial pneumonia of infancy in siblings. | we describe two brothers born 16 months apart who were hospitalized with culture-proved chlamydial pneumonia of infancy due to the same immunotype of chlamydia trachomatis. presumably the mother had either been chronically infected or had been reinfected. chlamydial pneumonia of infancy is a preventable disease if genital infection in pregnant women is diagnosed and treated. however, if pregnant women at high risk are not screened for c. trachomatis, multiple offspring may acquire the infection ... | 1988 | 3227475 |
| excision of cutaneous tumors in the horse using histologic guidance. | a highly successful surgical technique for removing cutaneous tumors in humans was used in seven horses with cutaneous squamous cell carcinoma (n = 3) or sarcoid (n = 4). in humans, the complete underside of the tumor is evaluated histologically by processing horizontal sections from the tumor base, and orientation between the wound surface and the undersurface of the excised tumor is maintained by mapping both surfaces. the technique ensures that small foci of residual tumor can be located accu ... | 1988 | 3227634 |
| effects of endotoxemia on systemic plasma loss and hematocrit in rats. | endotoxemia in rats increases plasma extravasation but does not result in continuously rising hematocrit. these contradictory observations led us to design a study in anesthetized rats (c, control rats, n = 10; e, endotoxin rats, n = 10) in which we continuously measured in blood hematocrit (conductivity cell) and changes in concentration of 125i-hsa (human serum albumin) and 51cr-labeled red cell (51cr-rbc; multichannel analyzer) in an extracorporeal circuit. in two additional series of experim ... | 1988 | 3231125 |
| effect of ph, nacl, temperature and time of incubation on the probability of vibrio parahaemolyticus growth initiation in a model broth. | | 1988 | 3232624 |
| consequences for swine farm income of depopulation as a disease control measure. | | 1988 | 3232640 |
| individual free fatty acids and lactate uptake in the human heart during severe sepsis. | coronary haemodynamics and myocardial metabolism of nonesterified fatty acids (nefa) and lactate were studied in 11 patients with severe sepsis, and compared to 10 control subjects. coronary sinus blood flow was evaluated by thermodilution. arterial and coronary sinus blood samples were collected for the measurement of lactate and total and individual nefa concentrations both in septic and control patients. there was an increase in lactate and total nefa arterial concentrations with a marked inc ... | 1988 | 3232957 |
| [kidney morphology in hemolytic-uremic syndrome]. | morphology of the kidneys in hemolytic-uremic syndrome is considered basing on autopsy findings obtained for 3 infants with 5-17-day history of acute renal failure. a newborn infant of 17 days developed the disease after feto-fetal hemotransfusion when macerated fetus-donor hemolysis products entered the circulation of the fetus-recipient through monochorionic placenta. the second case in an infant of 6 months was due to adtp vaccine. the last infant aged 16 months manifested the syndrome in the ... | 1988 | 3233069 |
| [effectiveness of distal gene translation in polycistrons depends upon the arrangement of regulatory signals on a template]. | the role of the translational terminator and initiator signals arrangement for two adjacent genes in polycistronic mrna has been studied. semisynthetic beta-galactosidase gene (lacz) of e. coli and fragment of phage m13 dna (with promoter pviii, gene ix, and part of gene viii) were used for constructing of the ix-viii-lacz artificial polycistronic operon. cloning of the constructs into pbr322 vector resulted in a number of plz381n plasmids differing by the mutual arrangement of gene viii transla ... | 1988 | 3233097 |
| partial characterization of a fraction from human follicular fluid that initiates the human sperm acrosome reaction in vitro. | a human follicular fluid (hff) fraction prepared by sephadex g-75 column chromatography has been previously shown by this laboratory to initiate the human sperm acrosome reaction (ar) in vitro. in the present report, the apparent molecular weight (mw) of this ar activity determined by a longer g-75 column than was used in the previous work was 50,000 +/- 5,106. the g-75 sephadex void volume fractions of some but not all hff samples were also found to contain some ar-initiating activity. the occa ... | 1988 | 3235026 |
| effect of ionizing radiation on macrophage stimulating property of vibrio parahaemolyticus lipopolysaccharide. | | 1988 | 3243588 |
| the effects of anions on fluid reabsorption from the proximal convoluted tubule of the rat. | 1. fluid reabsorption from surface proximal tubules of the rat was measured in vivo using stationary microperfusion techniques. reabsorptive rate (jv) was measured from droplets containing chloride as the main reabsorbable anion and when chloride was substituted by bromide, iodide, nitrate, acetate, isethionate or methylsulphate in either the tubular lumen alone or in both lumen and peritubular capillaries. 2. in tubules with an intact blood supply, droplet volume decreased in a manner best desc ... | 1988 | 3256612 |
| on the time-dependent diffusion of macromolecules through transient open junctions and their subendothelial spread. i. short-time model for cleft exit region. | in this two-part study we shall quantitatively study, using time-dependent models, the hypothesis that transient open junctions associated with widely scattered endothelial cells undergoing mitosis are the structural equivalent for the large pore pathway via which macromolecules the size of albumin or larger cross the vascular endothelium. in an earlier steady-state model [am. j. physiol. 248, h945-960 (1985)], the authors demonstrated that such an open-junction pathway could quantitatively acco ... | 1988 | 3256708 |
| [a lipoma of the cerebellopontile angle]. | the case of a 38-year old man with a lipoma of the left cerebellopontine angle is presented. while the clinical signs of that tumour are unspecific, an exact diagnosis is possible by ct and mri. as in other reported cases only an incomplete removal of the tumours was made because of the adherence of the lipoma to the nerves of the cpa. | 1988 | 3261397 |
| [a lipoma of the cerebellopontile angle]. | the case of a 38-year old man with a lipoma of the left cerebellopontine angle is presented. while the clinical signs of that tumour are unspecific, an exact diagnosis is possible by ct and mri. as in other reported cases only an incomplete removal of the tumours was made because of the adherence of the lipoma to the nerves of the cpa. | 1988 | 3261397 |
| interleukin 4 promotes the growth of tumor-infiltrating lymphocytes cytotoxic for human autologous melanoma. | addition of il-4 (1,000 u/ml) to either high or low concentrations of il-2 augmented tumor-infiltrating lymphocytes (til) growth from human melanoma. weekly restimulation with irradiated tumor cells in conjunction with il-4 allowed enhanced growth of til. with low-dose il-2 (10 u/ml) and il-4, expanded til had little cytolytic activity against daudi or allogeneic tumors. further, il-4 augmented the total lytic activity against autologous tumors in most cases. with high-dose il-2 (1,000 u/ml), il ... | 1988 | 3264324 |
| malaria infection in rats with stimulated splenic red pulp: the blood flow and protective effect in normal and transplanted splenic tissue. | after splenic autotransplantation both weight and blood flow of the regenerated splenic tissue are decreased. in addition, the protective function of the transplant is less compared to that of the normal spleen. in the present study, the red pulp of normal and transplanted splenic tissue was stimulated by injections of phenylhydrazine to increase the weight, the blood flow, and the protective function. after stimulation, the weight of the normal spleen increased to 900 +/- 70 mg (control 530 +/- ... | 1988 | 3265528 |
| megakaryocyte colony-stimulating factor and burst-promoting activity in lps-treated mouse spleen cell-conditioned medium. | in order to clarify the mechanism(s) of increased splenic hematopoiesis noted in lipopolysaccharide (lps)-injected mice, the effects of spleen cell-conditioned medium (spcm) on megakaryocyte colony (cfu-meg) formation and early erythroid (bfu-e) differentiation were investigated. after spleen cells from lps-injected mice were incubated for 3 days, the spcm was assayed for megakaryocyte colony-stimulating factor (meg-csf) in cfu-meg assay and for burst-promoting activity (bpa) and erythropoietin ... | 1988 | 3265923 |
| effect of recent antibacterial agents against bacteria causing diarrhoea. | the susceptibility of 424 bacterial isolates causing diarrhoea were tested by agar dilution technique on mueller-hinton agar against amoxicillin, ampicillin, ceftriaxone, chloramphenicol, co-trimoxazole, ciprofloxacin, doxycycline, norfloxacin and ofloxacin. the bacterial species included were aeromonas hydrophila, edwardsiella tarda, pleisomonas shigelloides, salmonella spp., shigella spp., vibrio cholerae and vibrio parahaemolyticus. the most active compounds were the fluorinated 4-quinolones ... | 1988 | 3266900 |
| [dna-dna homology and phenotypic characterization of environmental strains of vibrio parahaemolyticus and vibrio pelagius]. | a taxonomic study by means of dna homology and the most recent phenetic criteria has been carried out in 11 environmental strains of vibrio parahaemolyticus and 4 of vibrio pelagius, previously ascribed to the respective phenons in a numerical taxonomy study. the strains of v. parahaemolyticus showed genetic homogeneity, a guanine plus cytosine content (g + c) of 46% and 61% homology with v. parahaemolyticus type strain attc 17802. they exhibited phenetic atypicalities but could be ascribed to t ... | 1986 | 3271060 |
| dose-dependent effects of oral and intravenous glucose on insulin secretion and clearance in normal humans. | insulin secretion and clearance were studied in 2 groups of 7 normal subjects who each received 25, 50, and 100 g of glucose either orally or intravenously (iv) on separate occasions. insulin secretion rates were calculated during a 1-h base line and for 5 h after glucose administration from a two-compartmental analysis of peripheral c-peptide concentrations using individual kinetic parameters derived after iv bolus injections of biosynthetic human c-peptide. incremental glucose areas after oral ... | 1988 | 3279811 |
| evaluation of four methods for enumeration of vibrio parahaemolyticus. | two membrane filter (mf) and two most-probable-number methods for enumerating vibrio parahaemolyticus were compared. the mf methods used elevated-temperature incubations (41 and 42 degrees c) and were more specific than the most-probable-number methods (conducted at 35 degrees c). the mf method with a hydrophobic grid and a repair step was most effective. | 1988 | 3281584 |
| [immunodiagnosis of aids using preparations of the recombinant antigen of the surface protein of the htlv-iii virus]. | a technological scheme has been developed for purification of recombinant antigen of surface protein (asp) of the causative virus of aids from escherichia coli cells carrying plasmid pl2 which codes for the synthesis of a hybrid polypeptide consisting of phage lambda n protein (59 amino acid residues) and a fragment of sp (env) of hiv virus (569 a.r.). purification of asp is based on two separation principles: fractionation of polypeptides of bacterial lysates by preparative isoelectrofocusing i ... | 1988 | 3285593 |
| valanimycin acts on dna in bacterial cells. | | 1987 | 3294774 |
| colocalization of microtubule-associated protein 1a and microtubule-associated protein 2 on neuronal microtubules in situ revealed with double-label immunoelectron microscopy. | microtubule-associated protein 1a (map1a) and microtubule-associated protein 2 (map2) were shown to be colocalized on the same microtubules (mts) within neuronal cytoskeletons by double-label immunoelectron microscopy. to investigate the electron microscopic disposition of map1a and map2 and their relationship to mts in vivo, and to determine whether there are different subsets of mts which specifically bind either map1 or map2, we employed a double-label immunogold procedure on rat cerebella us ... | 1987 | 3294858 |
| x-linked gene expression and x-chromosome inactivation: marsupials, mouse, and man compared. | the existence of paternal x inactivation in australian and american marsupial species suggests that this feature of x-chromosome dosage compensation is not a recent adaptation, but probably predates the evolutionary separation of the australian and american marsupial lineages. although it is theoretically possible that the marsupial system is one of random x inactivation with p greater than 0.99 and q less than 0.01 and dependent on parental source, no instance of random x inactivation (p = q or ... | 1987 | 3298156 |
| comparison between proteinases of human seminal plasma and of sperm origin. | a comparison of the alkaline proteinase activity of human seminal plasma, the seminal non-gamete cellular material and spermatozoa was made by gelatin-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (gelatin-sds-page) zymography. several major (molecular weights = greater than 56,000) and minor (35,000 to 44,000) bands of proteinase activity were seen in the seminal plasma samples from nonvasectomized and vasectomized, healthy donors. similar activity profiles were observed in the nong ... | 1987 | 3305451 |
| foodborne diseases in india. | | 1987 | 3308696 |
| effect of acute renal failure on insulin disposition in the isolated perfused rat kidney. | the renal disposition of insulin in acute renal failure has not been evaluated. we used the isolated perfused rat kidney to test the hypothesis that acute renal failure (arf) decreases renal insulin clearance. we used warm ischemia for 45 min, uranyl nitrate 5 mg/kg, ureter ligation, and nonfiltering kidneys as methods of inducing arf. comparisons were made with normal control kidneys. the concentrations of insulin in perfusate and urine was determined by radioimmunoassay. acute renal failure ca ... | 1987 | 3318499 |
| [the effect of an alpha-glucosidase inhibitor on fatty liver in obesity]. | | 1987 | 3320414 |
| ascending urinary tract infections in rats induced by staphylococcus saprophyticus and proteus mirabilis. | ascending pyelonephritis was induced by staphylococcus saprophyticus and proteus mirabilis both by inoculating the organisms separately and in combination into rat bladders. bacterial cultures of tissue homogenates showed that pyelonephritis by both bacteria occurred significantly more often in rats where the two organisms were instilled concomitantly, suggesting a synergistic virulence between the two species. the antibody response to the different organisms was the same either the bacteria wer ... | 1987 | 3326399 |
| ascending urinary tract infections in rats induced by staphylococcus saprophyticus and proteus mirabilis. | ascending pyelonephritis was induced by staphylococcus saprophyticus and proteus mirabilis both by inoculating the organisms separately and in combination into rat bladders. bacterial cultures of tissue homogenates showed that pyelonephritis by both bacteria occurred significantly more often in rats where the two organisms were instilled concomitantly, suggesting a synergistic virulence between the two species. the antibody response to the different organisms was the same either the bacteria wer ... | 1987 | 3326399 |
| [cloning and expression in escherichia coli of vibrio parahaemolyticus thermostable direct hemolysin and thermolabile hemolysin genes]. | | 1987 | 3329243 |
| magnetic resonance imaging of ischemic bowel in rabbit model. | acute mesenteric ischemic bowel disease is a common yet complex disorder with high morbidity and mortality rates predominantly caused by delayed diagnosis. we examined the role of magnetic resonance imaging (mri) in the early detection of small bowel ischemia using the rabbit model. surgical ligation of the appropriate arterial vascular supply to the ileum of 10 rabbits produced the ischemic compromise. the animals were imaged at different time intervals after the arterial occlusion. multislice, ... | 1988 | 3343117 |
| complete separation of nine equine oestrogens by high-performance liquid chromatography. | | 1988 | 3346348 |
| estrogen- and antiestrogen-induced ornithine decarboxylase activity and uterine growth in the rat. | the estrogen antagonists tamoxifen and monohydroxytamoxifen are also classified as partial estrogen agonists. in infantile rats, estradiol induced a single peak of uterine odc activity at 6h following injection regardless of the extent of induction by various estradiol doses. by contrast, the timing of the odc activity peak induced by tamoxifen and monohydroxytamoxifen was highly dependent upon the dosing conditions and was delayed to 18 h at lower tamoxifen doses. in immature rats, tamoxifen an ... | 1988 | 3347055 |
| [a patient with acute secretory diarrhea caused by an autochthonous infection with vibrio parahaemolyticus]. | | 1988 | 3347287 |
| a theoretical framework for evaluation and treatment of the victims of child sexual assault by a nonfamily member. | thirty-seven families whose children were victims of sexual abuse by a nonfamily member were evaluated and treated during a nine-month period. the average age of the children was 5.36 years. the youngest children were boys, and all the adolescents were girls. a theoretical framework adapted from ferreira's and byng-hall's work was developed to assess and treat child victims of sexual molestation and their families. the format consists of three concepts. first, the trauma from sexual abuse, being ... | 1988 | 3360096 |
| purification and characterization of a family of high molecular weight surface-array proteins from campylobacter fetus. | a variety of gram-negative and gram-positive bacteria possess crystalline surface layers, although little is known of their function. we previously have shown that the high molecular weight surface-array proteins of campylobacter fetus are important in both the pathogenicity and antigenicity of this organism. for biochemical and immunological characterization, we purified high molecular weight (100,000, 127,000, 149,000) surface-array proteins from three c. fetus strains using sequential gel fil ... | 1988 | 3360785 |
| high expression of functional adenovirus dna polymerase and precursor terminal protein using recombinant vaccinia virus. | initiation of adenovirus (ad) dna replication occurs by a protein-priming mechanism in which the viral precursor terminal protein (ptp) and dna polymerase (pol) as well as two nuclear dna-binding proteins from uninfected hela cells are required. biochemical studies on the ptp and dna polymerase proteins separately have been hampered due to their low abundance and their presence as a ptp-pol complex in ad infected cells. we have constructed a genomic sequence containing the large open reading fra ... | 1988 | 3362670 |
| a comparison of the effects of measured, predicted, estimated and constant residual volumes on the body density of female athletes. | the body density (bd), and hence the relative body fat (% bf) was measured for 182 female athletes. the residual volume (rv) was determined both before and after the underwater weighing by a multiple breath helium dilution technique with the subject immersed to neck level. the absolute mean difference (lxdl) and see between the two rv trials were 63 and 75 ml, respectively. these increased to values ranging 144-685 and 187-252 ml, respectively, when the mean of the two rv trials for each subject ... | 1988 | 3366515 |
| time course of adaptation to hypoxia in newborn rats. | in newborn rats after a few minutes of hypoxia, ventilation is similar to the normoxic value. nevertheless, after a few days in hypoxia, newborn rats have a sustained hyperventilation. in this study we examined the time course of the newborn rat's adaptation to hypoxia. measurements of body size, hematocrit, lung and heart mass, and breathing pattern have been performed on newborn rats exposed to hypoxia (10% o2) for different time intervals from 4 to 60 h (hypoxic, h), and on same-age rats grow ... | 1988 | 3370546 |
| occurrence of vibrionaceae in natural and cultivated oyster populations in the pacific northwest. | studies were done to assess the role of oysters from the pacific northwest as a potential source of vibrionaceae. oysters collected from natural and cultivated populations on the british columbia coast were opened using sterile instruments. the gills and oyster meat were each removed, and the meat was cut in half. the gills and the cut surface of the oyster meat were each cultured by inoculating them directly to the surface of agar plating media for the isolation of vibrionaceae. overall, 120 oy ... | 1988 | 3370928 |
| vibrio parahaemolyticus (kanagawa-negative) wound infection in a hospital dietary employee. | vibrio parahaemolyticus is an estuarine bacterium that is a major cause of gastroenteritis worldwide. occasionally, this organism has also been shown to be the causative agent of wound infections. in almost all cases of gastrointestinal disease only strains that produce hemolysin ("kanagawa-positive") are implicated. we have isolated a kanagawa-negative strain of v. parahaemolyticus from serous wound drainage of the infected foot of a hospital dietary employee. to our knowledge, this is only the ... | 1988 | 3370930 |
| effects of lignocaine and bupivacaine on regional myocardial function and coronary blood flow in anaesthetized dogs. | empirical i.v. doses of lignocaine or bupivacaine of equal local anaesthetic potency were administered to halothane-anaesthetized dogs. both local anaesthetics caused the expected depression of global haemodynamic function. regional myocardial systolic shortening was depressed similarly by both agents. regional myocardial dysfunction, seen as post-systolic shortening, occurred to a similar extent with both lignocaine and bupivacaine. coronary blood flow and coronary perfusion pressure were signi ... | 1988 | 3377951 |
| protein kinase c in the developing rat liver, heart and brain. | ontogenic changes of protein kinase c in the rat liver, heart and cerebrum were examined from 17-day gestation until adult. cerebral protein kinase c activity was 19 times less at 17-day fetal as compared to 15-day postnatum or older rats. the enzyme activity in the heart was generally higher in the neonatal period than the adult, but was not correlated with the previously reported alpha 1-adrenergic and cholinergic receptor ontogeny. likewise in the liver, the enzyme activity was not correlated ... | 1988 | 3383720 |
| risk assessment for acquiring meningitis tuberculosis among children not vaccinated with bcg: a case-control study. | this case-control study was conducted to assess the risk, among children aged 0-12 years, of developing meningitis tuberculosis (mt) associated with a lack of intradermal bcg vaccination. cases (45) of mt admitted for treatment at the fundacao benjamin guimaraes hospital (belo horizonte, mg, brazil), from 1975 to 1981, were matched for age at hospitalization, date of hospitalization and nutritional status, with two types of controls--patients with acute diarrhoea (ad) and patients with acute non ... | 1988 | 3384537 |
| postoperative wound infection associated with vibrio parahaemolyticus in a patient without exposure to seawater. | this report describes a case of wound infection associated with vibrio parahaemolyticus. the patient had ingested steamed crabs 7 days before admission for surgical treatment of intestinal obstruction due to colon carcinoma. the vibrio sp. was isolated from postoperative wound drainage as well as from stool. recovery was uneventful. | 1988 | 3384932 |
| progesterone regulation of secretory component (sc): uterine sc response in organ culture following in vivo hormone treatment. | sex steroid hormones are known to have profound effects on mucosal immunity. in the present study we evaluated the effects of progesterone on the uterine immune system by determining the changes in the levels of secretory component (sc) released from uterine tissues in culture following in vivo administration of progesterone to estradiol-stimulated ovariectomized rats. sc is a transport protein which moves iga into external secretions such as intestinal and uterine secretions. sc release was det ... | 1988 | 3386246 |
| australian fraud inquiry under way. | | 1988 | 3386761 |
| dissociation of hcg-receptor complex and detection of hcg receptors in human chorionic tissues. | we developed a radioreceptor assay (rra) system for investigating occupied hcg receptors by using a rat testis membrane fraction. treatment with dithiothreitol, neuraminidase, and low ph (3.3) buffer partially dissociated hcg from its receptor with dissociation rates of 42.8%, 35.2%, and 68.7%, respectively. specific receptor rebinding was unaffected and showed competitive inhibition of [125i]hcg rebinding by unlabeled hcg. scatchard plot analysis yielded a rebinding km of 1.798-3.496/pm. high c ... | 1988 | 3392440 |
| biological activities of lipid a from vibrio parahaemolyticus: stimulation of murine peritoneal macrophages. | the toxicity and macrophage stimulating property of vibrio parahaemolyticus lipid a was studied. the ld50 dose of lipid a in galactosamine-sensitized mice was found to be 0.6 micrograms when injected intraperitoneally. administration of lipid a resulted in stimulation of peritoneal macrophages as evident by increase in their cellular rna contents and lysosomal enzyme activities. the treatment also caused enhancement in the phagocytic activity of macrophages. | 1988 | 3393096 |
| attachment of a vibrio parahaemolyticus strain to rabbit brush border membranes. | the attachment of a clinical isolate of vibrio parahaemolyticus to brush border membranes isolated from rabbits was studied, using transmission electron microscopy. the bacterial cells adhered to the brush borders, apparently with a small space between the cell wall and the surface of the microvilli. no extracellular structures were seen to be involved in the process of adhesion. | 1988 | 3394451 |
| [the differentiation of bordetella bronchiseptica strains. 7. phenotypic changes in bordetella bronchiseptica through changes of phase]. | | 1988 | 3395180 |
| flagellar dynamometer controls swarmer cell differentiation of v. parahaemolyticus. | swarmer cell genes, laf, are induced when v. parahaemolyticus is grown on the surface of solidified media, embedded in solidified media, suspended in viscous media, or agglutinated with antibody in liquid media. these conditions have in common the constraint of the movement of the polar flagellum. to test the hypothesis that the polar flagellum functions as a sensor necessary for control of swarmer cell formation, we have constructed a variety of mutations in genes encoding components of the pol ... | 1988 | 3396074 |
| [allergen analysis of mugwort pollen (artemisia vulgaris) using crossed radioimmunoelectrophoresis and radioallergosorbent test inhibition]. | it could be identified 43 antigens of mugwort pollen (artemisia vulgaris), 31 migrating anodically and 12 cathodically, by means of crossed immunoelectrophoresis (cie). eleven antigens bound human ige-antibodies in crossed radioimmunoelectrophoresis (crie). two major allergens could be found, allergens 23 and 40.- the allergenic activity of mugwort pollen extract was examined by means of rast-inhibition. specificity of this reaction could be determined by a control trial with grass pollen extrac ... | 1988 | 3400558 |
| influence of macrogol bases on the bioavailability of bacampicillin after rectal administration in rabbits. | | 1988 | 3416367 |
| experimental contact sensitization with 3,4,5-trichloropyridazine. | the potential of 3,4,5-trichloropyridazine to induce contact sensitization was assessed in the guinea pig maximization test of magnusson and kligman and also in the closed patch test described by buehler. the test material was a 1% solution of 3,4,5-trichloropyridazine in a highly refined mineral oil. the test material elicited moderate to severe irritation when diluted in mineral oil to concentrations of 15-25% and minimal irritation at concentrations of 1-3%. both tests clearly indicated that ... | 1988 | 3416588 |