| simultaneous quantification of escherichia coli o157:h7 and shigella boydii using a visual-antibody-macroarray. | being high throughput, rapid, automated, economical, convenient to operate and highly sensitive, protein arrays have been widely used in the analysis of tumor markers and veterinary drug residues. pathogenic microbes also can be detected qualitatively by dna array or protein array; however, their high throughput detection and quantification remains a big obstacle. to evaluate the potentiality of protein arrays for multiple quantitative detection of microorganisms with naked eye examination witho ... | 2015 | 26300105 |
| intracellular and membrane-damaging activities of methyl gallate isolated from terminalia chebula against multidrug-resistant shigella spp. | shigella spp. (shigella dysenteriae, shigella flexneri, shigella boydii and shigella sonnei) cause bacillary dysentery (shigellosis), which is characterized by bloody mucous diarrhoea. although a variety of antibiotics have been effective for treatment of shigellosis, options are becoming limited due to globally emerging drug resistance. in the present study, in vitro antibacterial activity of methyl gallate (mg) isolated from terminalia chebula was determined by performing mic, minimal bacteric ... | 2015 | 26272388 |
| molecular genotyping of shigella sonnei strains isolated from children with bloody diarrhea using pulsed field gel electrophoresis on the total genome and pcr-rflp of ipah and ipabcd genes. | identification, understanding of antibiotic sensitivity patterns and molecular characterization of genetic elements of shigella species are important because of both epidemiological and clinical indications in developing countries. | 2015 | 25789126 |
| optimization of electrically active magnetic nanoparticles as accurate and efficient microbial extraction tools. | food defense requires the means to efficiently screen large volumes of food for microbial pathogens. even rapid detection methods often require lengthy enrichment steps, making them impractical for this application. there is a great need for rapid, sensitive, specific, and inexpensive methods for extracting and concentrating microbial pathogens from food. in this study, an immuno-magnetic separation (ims) methodology was developed for escherichia coli o157:h7, using electrically active magnetic ... | 2015 | 25664527 |
| shigella outer membrane protein pssp-1 is broadly protective against shigella infection. | in developing countries, shigella is a primary cause of diarrhea in infants and young children. although antibiotic therapy is an effective treatment for shigellosis, therapeutic options are narrowing due to the emergence of antibiotic resistance. thus, preventive vaccination could become the most efficacious approach for controlling shigellosis. we have identified several conserved protein antigens that are shared by multiple shigella serotypes and species. among these, one antigen induced cros ... | 2015 | 25651919 |
| characterization and complete genome sequence of a novel n4-like bacteriophage, psb-1 infecting shigella boydii. | shigellosis is one of major foodborne pathogens in both developed and developing countries. although antibiotic therapy is considered an effective treatment for shigellosis, the imprudent use of antibiotics has led to the increase of multiple-antibiotic-resistant shigella species globally. in this study, we isolated a virulent podoviridae bacteriophage (phage), psb-1, that infects shigella boydii. one-step growth analysis revealed that this phage has a short latent period (15 min) and a large bu ... | 2014 | 25283727 |
| molecular characterization of serologically atypical provisional serovars of shigella isolates from kolkata, india. | during 2000-2004, 13 shigella strains that were untypable by commercially available antisera were isolated from children <5 years of age with acute diarrhoea in kolkata. these strains were subsequently identified as shigella dysenteriae provisional serovar 204/96 (n = 3), shigella dysenteriae provisional serovar e23507 (n = 1), shigella dysenteriae provisional serovar i9809-73 (n = 1), shigella dysenteriae provisional serovar 93-119 (n = 1), shigella flexneri provisional serovar 88-893 (n = 6) a ... | 2014 | 25261061 |
| isolation and development of bioluminescent reporter phages for bacterial dysentery. | shigellosis is a significant cause of morbidity and mortality worldwide, most notably amongst children. moreover, there is a global increase in the occurrence of multidrug-resistant isolates, including the epidemic and pandemic shigella dysenteriae type 1 strain. we developed a bioluminescent reporter phage assay to facilitate detection and simultaneously determine antibiotic susceptibility. a shigella flexneri phage (shfl25875) was isolated from environmental wastewater and characterized by dna ... | 2015 | 25252629 |
| protective immunity by oral immunization with heat-killed shigella strains in a guinea pig colitis model. | the protective efficacy of and immune response to heat-killed cells of monovalent and hexavalent mixtures of six serogroups/serotypes of shigella strains (shigella dysenteriae 1, shigella flexneri 2a, s. flexneri 3a, s. flexneri 6, shigella boydii 4, and shigella sonnei) were examined in a guinea pig colitis model. a monovalent or hexavalent mixture containing 1 × 10(7) of each serogroup/serotype of heat-killed shigella cells was administered orally on days 0, 7, 14 and 21. on day 28, the immuni ... | 2013 | 24033533 |
| characterization and complete genome sequence of the shigella bacteriophage psf-1. | shigellosis is a global health problem, and shigella flexneri is the major cause of this disease. in this study, we isolated a virulent siphoviridae bacteriophage (phage), psf-1, that infects s. flexneri. this phage was isolated from the han river in korea and was found to infect s. flexneri, shigella boydii, and shigella sonnei. one-step growth analysis revealed that this phage has a short latent period (10 min) and a large burst size (86.86 pfu/cell), indicating that psf-1 has good host infect ... | 2013 | 24012542 |
| multi-serotype outer membrane vesicles of shigellae confer passive protection to the neonatal mice against shigellosis. | recently, we have demonstrated, immunization of adult female mice with outer membrane vesicles (omvs) of shigella boydii type 4 protected their offspring passively from shigellosis. in our present study, we have advanced our research by formulating multi-serotype outer membrane vesicles (momvs), mixing the omvs of shigella dysenteriae 1 δstx, shigella flexneri 2a, 3a and 6, s. boydii type 4 and shigella sonnei to achieve a broad spectrum protection against shigellosis. adult mice were immunized ... | 2013 | 23684822 |
| phage inactivation of foodborne shigella on ready-to-eat spiced chicken. | shigellosis, also called bacillary dysentery, is an infectious disease caused by shigella species, including shigella flexneri, shigella dysenteriae, shigella sonnei, and shigella boydii. infection with s. flexneri can result in epidemics, and shigella-contaminated food is often the source of infection, such as ready-to-eat spiced chicken and duck. therefore, we investigated the ability of shigella phages to inhibit pathogenic shigella spp. in ready-to-eat spiced chicken. food samples were inocu ... | 2013 | 23243250 |
| studies on shigella boydii infection in caenorhabditis elegans and bioinformatics analysis of immune regulatory protein interactions. | shigella boydii causes bacillary dysentery or shigellosis and generates a significant burden in the developing nations. s. boydii-mediated infection assays were performed at both physiological and molecular levels using caenorhabditis elegans as a host. continuous exposure of worms to s. boydii showed a reduced life span indicating the pathogenicity of shigella. quantitative real-time pcr analysis was performed to analyze the expression and regulation of host specific candidate-antimicrobial gen ... | 2012 | 22841995 |
| outer membrane vesicles of shigella boydii type 4 induce passive immunity in neonatal mice. | like most other gram-negative bacteria, shigella releases outer membrane vesicles (omvs) into the surrounding environment during growth. in this study, we have exploited omvs of shigella as a protective immunogen in a mice model against shigellosis. distinctive vesicle secretion was noticed from different shigella strains. among them, shigella boydii type 4 (bch612) was secreting relatively higher amounts. we immunized female adult mice orally with 32 μg of purified shigella boydii type 4 (bch61 ... | 2012 | 22762732 |
| effect of bovine lactoferrin on the minimum inhibitory concentrations of ampicillin and trimethoprim-sulfamethoxazole for clinical shigella spp. strains. | here, we determined the effect of bovine lactoferrin (blf) on the minimum inhibitory concentration (mic) of ampicillin and trimethoprim-sulfamethoxazole in shigella . using a microdilution method, the mic was determined in the presence or absence of bovine lactoferrin (10 mg/ml) on 88 shigella strains (56 shigella flexneri , 15 shigella boydii , 13 shigella sonnei , and 4 shigella dysenteriae ) previously isolated from peruvian children <2 years old. a fold change of 2 or more in mic values was ... | 2012 | 22397495 |
| antimicrobial-resistant shigella infections from iran: an overlooked problem? | in this study, we wanted to assess the level of antimicrobial resistance, the presence of genes encoding resistance to cephalosporins and plasmid-mediated quinolone resistance (pmqr), and genetic relatedness among shigella isolates obtained from iranian patients. | 2012 | 22345385 |
| comparison of minimum inhibitory concentration of water soluble extracts of eugenia jambolana lam. (fam. myrtaceae) barks of different ages on dysentery and diarrhoea forming micro - organisms. | a preliminary investigations was carried out to study the antibacterial activity of the water soluble extracts of five and ten years old barks of eugenia jambolana lam. (fam. myrtaceae) on dysentery and diarrhoea forming micro organisms. it was observed that the barks of young plants have a better inhibitory effect on micro - organisms like salmonella viballerup, shigella dysenteriae 10, shigella boydii 5, sgigella dysenteriae 2. | 1985 | 22557509 |
| in vitro anti-oxidant and anti-microbial potentiality investigation of different fractions of caryota urens leaves. | caryota urens is a member of the arecaceae family and a common plant in the southeast asian region. this plant has been reported as an anti-microbial agent in recent years. thus, we aimed to find out the mic (minimum inhibitory concentration) against different pathogenic microorganism. | 2016 | 28536384 |
| the enterohemorrhagic escherichia coli effector espw triggers actin remodeling in a rac1-dependent manner. | enterohemorrhagic escherichia coli (ehec) is a diarrheagenic pathogen that colonizes the gut mucosa and induces attaching-and-effacing lesions. ehec employs a type iii secretion system (t3ss) to translocate 50 effector proteins that hijack and manipulate host cell signaling pathways, which allow bacterial colonization and subversion of immune responses and disease progression. the aim of this study was to characterize the t3ss effector espw. we found espw in the sequenced o157:h7 and non-o157 eh ... | 2017 | 28630074 |