| [physicochemical and biological characteristics of preparations of an antigenic complex isolated from the cultivation medium of bordetella pertussis]. | the physicochemical and biological properties of antigenic complexes isolated from the supernatant fluid of the culture medium of b. pertussis, strains 305 and 475, were studied. the preparations obtained from both strains contained proteins, carbohydrates, nucleic acids and lipids. electrophoresis in polyacrylamide gel revealed the presence of filamentous hemagglutinin, 4 subunits of b. pertussis toxin and agglutinogens in the antigenic complexes of both strains. the preparations of both strain ... | 1988 | 2904201 |
| lipopolysaccharides in a traditional pertussis vaccine. | analysis of the lipopolysaccharide (lps, endotoxin) in cell sonicates of four danish vaccine strains of bordetella pertussis (3803, 3825, 3843 and 3860) and of purified strain 3803 lps in sodium dodecyl sulphate-polyacrylamide gel electrophoresis by silver staining, showed identical profiles. the lps profile revealed a dominant, brownish lps ii band and a minor, faster-migrating, black-stained lps i band. however, the ratio of lps i to lps ii in the preparation of purified lps differed slightly ... | 1988 | 2904442 |
| whooping cough diagnosis: a clinical evaluation of complementing culture and immunofluorescence with enzyme-linked immunosorbent assay of pertussis immunoglobulin a in nasopharyngeal secretions. | pernasal aspirate (pna) was obtained from 543 children during a 6-month period when whooping cough was prevalent. three tests for diagnosing pertussis were performed on the pna: (a) examination of direct smears by immunofluorescence (if) for bordetella pertussis; (b) culture; and (c) estimation of b. pertussis-specific immunoglobulin-a antibody (p-iga) by an enzyme-linked immunosorbent assay (elisa). on clinical review, 395 children were assessed to have had pertussis (p children) and 148 childr ... | 1988 | 2904501 |
| expression and secretion of pertussis toxin subunit s1 in bacillus subtilis. | pertussis toxin (pt) is an important virulence determinant of bordetella pertussis and one of the major protective antigens against whooping cough. the genes coding for pt have recently been cloned, but attempts to express them in escherichia coli have been unsuccessful. we therefore explored the possibility of expressing these genes in bacilius subtilis for which efficient vectors are available. the lack of endotoxin in the gram-positive bacillus might be an additional advantage for the product ... | 1987 | 2904640 |
| the pathogenesis and immunology of vibrio cholerae and bordetella pertussis. | | 1988 | 2904796 |
| virulence factors of bordetella pertussis. | clearly, b. pertussis has evolved very elaborate mechanisms to maintain itself in the human host. three different proteins (fha, pertussis toxin and fimbriae) have been implicated in adherence. furthermore, a number of toxins are produced (pertussis toxin, adenylate cyclase, dermonecrotic toxin, and tracheal cytotoxin) which destroy the clearance mechanisms of the respiratory tract, or suppress the immune response. there is evidence that b. pertussis may survive intracellularly, and the possibil ... | 1988 | 2904800 |
| monocyte-macrophages increase in the bursal follicular medulla following bacterial intracloacal inoculation of bordetella pertussis in chicks. | | 1988 | 2905104 |
| relationships between the calmodulin-dependent adenylate cyclases produced by bacillus anthracis and bordetella pertussis. | the nucleotide sequences for the calmodulin-dependent adenylate cyclases produced by bordetella pertussis and bacillus anthracis have recently been determined. the gc% for the b. pertussis and b. anthracis cyclase genes are about 65% and 29%, respectively. despite this difference in nucleotide composition, these cyclases possess three highly conserved amino acid domains and share some nucleotide sequence homology. one of these conserved domains appears to be involved in atp binding and is relate ... | 1988 | 2905126 |
| secretion of cyclolysin, the calmodulin-sensitive adenylate cyclase-haemolysin bifunctional protein of bordetella pertussis. | the calmodulin-sensitive adenylate cyclase of bordetella pertussis, a 45 kd secreted protein, is synthesized as a 1706 amino acid precursor. we have shown that this precursor is a bifunctional protein, carrying both adenylate cyclase and haemolytic activities. the 1250 carboxy-terminal amino acids of the precursor showed 25% similarity with escherichia coli alpha-haemolysin (hlya) and 22% similarity with pasteurella haemolytica leucotoxin. three open reading frames were identified downstream fro ... | 1988 | 2905265 |
| structural homology between virulence-associated bacterial adenylate cyclases. | the primary structure of the calmodulin-sensitive adenylate cyclase toxin from bacillus anthracis has been determined from the corresponding nucleotide sequence and compared to that of the homologous toxin secreted by bordetella pertussis. the cya gene of bacillus anthracis encodes an 800 amino acid (aa) protein beginning with an n-terminal signal peptide. the central part of the b. anthracis adenylate cyclase includes a region of striking homology with the n-terminal part of the b. pertussis en ... | 1988 | 2906312 |
| use of supplemented stainer-scholte broth for the isolation of bordetella pertussis from clinical material. | the use of stainer-scholte broth supplemented with (2,6-o-dimethyl)beta-cyclodextrin (heptakis) for the isolation of bordetella pertussis from clinical specimens was evaluated with 3,632 nasal swabs from children and adults with suspected whooping cough or from their family contacts. the liquid enrichment medium was subcultured on charcoal agar with 10% defibrinated horse blood. charcoal agar and soft charcoal agar served as the standard procedure to detect b. pertussis. we isolated 772 strains ... | 1988 | 2906641 |
| comparison of modified bordet-gengou and modified regan-lowe media for the isolation of bordetella pertussis and bordetella parapertussis. | culture and fluorescent-antibody methods for detection of bordetella species were evaluated by two state public health laboratories. field-inoculated plates of regan-lowe agar medium were most useful if incubation was initiated on the day of collection. regan-lowe and bordet-gengou media were comparable for subculturing nasopharyngeal specimens that were transported and enriched in half-strength regan-lowe agar. maximum sensitivity was achieved when the media were used in parallel. fluorescent-a ... | 1988 | 2906642 |
| pertussis: the disease and new diagnostic methods. | bordetella pertussis, the causative agent of whooping cough, produces an acute and chronic respiratory infection in infants and young children. b. pertussis is still a major health problem of young children throughout the world even though effective immunization against whooping cough is available. while predominantly a childhood disease, it has been reported also to be a cause of persistent cough in adults. this review discusses the numerous bacterial virulence factors that may play roles in th ... | 1988 | 2906814 |
| phase variation in bordetella pertussis is accompanied by changes in dna modification. | pathogenic strains of bordetella pertussis tend to undergo a phase variation process when propagated in vitro. the phase variants do not express part or all of the virulence factors of the pathogenic strain and are phenotypically stable. we have previously shown that variation involves a non-reversible, non-random process. in an attempt to characterize the molecular changes accompanying phase variation, chromosomal dna, isolated from b. pertussis and its variants, was digested with a variety of ... | 1987 | 2907087 |
| characterization of fimbrial subunits from bordetella species. | using antisera raised against serotype 2 and 3 fimbrial subunits from bordetella pertussis, serologically related polypeptides were detected in bordetella bronchiseptica, bordetella parapertussis and bordetella avium strains. the two b. pertussis fimbrial subunits, and three of the serologically related b. bronchiseptica polypeptides, were shown to be very similar in amino acid composition and n-terminal amino acid sequence. homology was observed between the n-termini of these polypeptides, and ... | 1987 | 2907088 |
| cloning of the adenylate cyclase genetic determinant of bordetella pertussis and its expression in escherichia coli and b. pertussis. | a recombinant plasmid, prmb1, identified from a gene library of b. pertussis, restored adenylate cyclase (ac) and haemolysin (hly) activities to b. pertussis bp348 (a tn5-insertion mutant deficient in both these activities). b. pertussis bp348 was considerably less virulent than wild type strains of b. pertussis when 3-week-old mice were challenged intranasally; possession of prmb1 restored virulence. neither ac nor hly activities were expressed in e. coli harbouring prmb1. however, expression o ... | 1988 | 2907365 |
| studies on the optimal conditions for inactivation of bordetella pertussis organisms with glutaraldehyde for preparation of a safe and potent pertussis vaccine. | the optimal conditions for inactivation of bordetella pertussis organisms with glutaraldehyde for the production of a safe and potent whole cell pertussis vaccine were investigated. two bacterial harvests from b. pertussis strain 10536 were treated with glutaraldehyde, each with 0.025, 0.05 and 0.1% concentrations of glutaraldehyde for 10, 60 and 120 min. the nine types of glutaraldehyde-inactivated pertussis vaccine (gipv) and conventional heat-inactivated pertussis vaccine (hipv) preparations ... | 1988 | 2907704 |
| interaction of bordetella pertussis virulence components with neutrophils: effect on chemiluminescence induced by a chemotactic peptide and by intact bacteria. | the effect of secreted virulence components of bordetella pertussis on chemiluminescence (cl) of rabbit peritoneal neutrophils was determined with the chemotactic peptide n'-formyl-l-methionyl-l-leucyl-l-phenylalanine (fmlp) or intact b. pertussis as the stimulus. pertussis toxin (pt) inhibited the response to fmlp in a dose-dependent manner, although only after the neutrophils had been exposed to the toxin for greater than 15 min. both filamentous haemagglutinin (fha) and lipopolysaccharide (lp ... | 1988 | 2908118 |
| nucleotide sequence and characterization of a repetitive dna element from the genome of bordetella pertussis with characteristics of an insertion sequence. | a repeating element of dna has been isolated and sequenced from the genome of bordetella pertussis. restriction map analysis of this element shows single internal clai, sphi, bsteii and sali sites. over 40 dna fragments are seen in clai digests of b. pertussis genomic dna to which the repetitive dna sequence hybridizes. sequence analysis of the repeat reveals that it has properties consistent with bacterial insertion sequence (is) elements. these properties include its length of 1053 bp, multipl ... | 1988 | 2908119 |
| agglutinogens and fimbriae of bordetella pertussis. | agglutinogen 2 (agg2) of bordetella pertussis is a fimbrial antigen and therefore a potential adhesin and acellular vaccine component. agg2 was found to dissociate only under harsh conditions into the subunits of mol. wt. 22500 seen in sds-page. results from studies of agglutinogen 3 (agg3) are presented which confirm previous findings from this laboratory that agg3 is also a fimbrial protein but with a subunit mol. wt. of 22000. the amino acid sequence of agg2, deduced from the nucleotide seque ... | 1988 | 2908520 |
| structural and functional properties of a 69-kilodalton outer membrane protein of bordetella pertussis. | a-69-kda outer membrane protein present on virulent bordetella pertussis cells is recognized by the agglutinating monoclonal antibodies bpe3, bpd8, and bpe8. the amino acid composition of this protein, purified from heat extracts of b. pertussis bp353 cells, is different from that of the two major fimbrial antigens of b. pertussis, which is consistent with its being a nonfimbrial protein based on other criteria. western blot analysis using the monoclonal antibody bpe3 demonstrated that a slightl ... | 1988 | 2908522 |
| the vir locus and phase-variation in bordetella pertussis. | by a phenomenon known as phase-variation bordetella pertussis is capable of changing between a virulent-phase in which multiple virulence-associated determinants are expressed, and an avirulent-phase in which the virulence-associated determinants are not expressed. mutations in the vir locus of b. pertussis have a similar effect. we have examined the state of the vir locus in each of a series of strains derived one from the other by phase-variation. we have found that a single base-pair change i ... | 1988 | 2908523 |
| molecular cloning and analysis of p. 69, a vir-controlled protein from bordetella pertussis. | the cloning sequencing and analysis of an important antigenic component of bordetella pertussis is described. the gene for p.69, in common with a variety of other so called "virulence" genes, (e.g., adenylate cyclase (ac), pertussis toxin (pt) and filamentous haemagglutinin (fha)), is under control of the vir locus. the protein p.69 is externally localised on cells and protein preparations are protective as judged by the mouse intra-cerebral challenge test. the gene encoding the p.69 antigen was ... | 1988 | 2908524 |
| bordetella pertussis pilin and pilin-like genes. | bordetella pertussis fimbriae, which mediate the adherence of the microorganism to the epithelium of the respiratory tract, elicit a protective immunogenic response which could be taken advantage of for the creation of an acellular vaccine against pertussis. a few of the fimbrial proteins have been partially characterized by immunological means which recently the cloning and characterization of pilin and pilin-like genes are allowing us to study their structure, regulation of expression during t ... | 1988 | 2908525 |
| bordetella pertussis adenylate cyclase: the gene and the protein. | using the adenylate cyclase-calmodulin interaction as a tool, the b. pertussis cya gene was cloned in a cya defective e. coli strain harbouring a plasmid which expressed high levels of calmodulin. the determination of the nucleotide sequence of the gene showed that adenylate cyclase is synthesized as a large precursor of 1706 amino acids. the calmodulin-stimulated catalytic activity resides in the amino-terminal 400 amino acids whereas the 1300 amino acid carboxy-terminal part of the precursor i ... | 1988 | 2908526 |
| human t-cell immunity against bordetella pertussis analyzed at clonal level. | cloned peripheral blood t lymphocytes from an immune donor were grown in interleukin 2 and tested for proliferation in response to inactivated bordetella species (b. pertussis, b. parapertussis and b. bronchiseptica) and mutants deficient for the expression of virulence-associated antigens. all the t-cell clones obtained were cd4+8- and recognized specifically the bordetella antigens when presented by autologous b cells. on the basis of the responsiveness to the whole inactivated bacteria, it wa ... | 1988 | 2908527 |
| characterization and clinical study on the acellular pertussis vaccine produced by a combination of column purified pertussis toxin and filamentous hemagglutinin. | bordetella pertussis tohama phase i was cultured in a 300-liter fermentor using a medium containing 0.1% heptakis (2,5-0-dimethyl) beta-cyclodextrin (mecd). pertussis toxin (pt) and filamentous hamagglutinin (fha) were purified using affinity and ion exchange gel column chromatographies. endotoxin contents of these antigens (10 micrograms pn/ml) were less than 10 nglps/ml. pt and fha were independently treated with formalin in the presence of amino acid and were mixed at a protein concentration ... | 1988 | 2908528 |
| further characterization of japanese acellular pertussis vaccine prepared in 1988 by 6 japanese manufacturers. | in 1974, we published a paper entitled "leukocytosis-promoting factor of bordetella pertussis. its identity with protective antigen". a preparation which was purified from the culture supernatant of bordetella pertussis phase i, tohama strain by consecutive steps of ammonium sulfate fractionation and sucrose density gradient centrifugation showed leukocytosis-promoting, histamine-sensitizing and hemagglutinating activities. the preparation consisted of two proteins: pertussis toxin (pt) and fila ... | 1988 | 2908530 |
| the quantitative assay of the clustering activity of the lymphocytosis-promoting factor (pertussis toxin) of bordetella pertussis on chinese hamster ovary (cho) cells. | an experimental design and a statistical method for the estimation of the clustering-response activity of lymphocytosis-promoting factor (lpf) in chinese hamster ovary cells growing in wells on a microplate were investigated. the scoring method introduced by ipsen was adopted to express the grade of the clustering response rather than the end-point method generally used. the scoring method was validated by statistical analyses. the grade of response varied with the location of the wells on a mic ... | 1989 | 2921253 |
| induction of experimental autoimmune uveoretinitis and pinealitis by irbp. comparison to uveoretinitis induced by s-antigen and opsin. | microgram quantities bovine irbp (interphotoreceptor retinoid binding protein) injected in freund's complete adjuvant induced severe autoimmune uveoretinitis and pinealitis in lewis rats. at low doses the onset was accelerated and intensified by co-injection of hemophilus pertussis bacteria. wistar, bn and pvg rats were less susceptible, while the eyes of athymic, nude rats did not respond. the disease developed similar to but faster than s-antigen-induced uveoretinitis, while its onset was one ... | 1986 | 2938889 |
| susceptibility of cartilage to damage by immunological inflammation. | injection of ovalbumin into subcutaneous air pouches prepared on the backs of rats previously sensitised to the antigen resulted in the induction of a small and transient accumulation of inflammatory fluid with a predominantly polymorph cell infiltrate. challenge of pouches of appropriately sensitised rats with bordetella pertussis vaccine, on the other hand, resulted in a larger and more prolonged accumulation of fluid and cells with a predominantly mononuclear presence. when intact homologous ... | 1986 | 2942490 |
| cellular interactions in the adoptive transfer of contact sensitivity: characterization of an antigen-nonspecific vicia villosa-adherent t cell needed for adoptive transfer into naive recipients. | the adoptive transfer of delayed-type hypersensitivity (dth) into naive recipients requires the interaction of two functionally distinct ly-1+ t cells: and i-j- cell effector cell for dth which transfers antigen-specific dth only into animals whose suppressive mechanisms have been compromised, and and i-j+ cell which alone never transfers dth but allows the transfer of dth by the i-j- dth effector cell into naive animals. we investigated the phenotypic and functional characteristics of the cell ... | 1986 | 2943803 |
| adoptive transfer of murine autoimmune orchitis to naive recipients with immune lymphocytes. | a protocol was developed for reproducibly transferring experimental autoimmune orchitis (eao) to naive recipient mice. cell donors were (c57bl/6 x a/j)f1 mice immunized about 14 days earlier with mouse testicular homogenate with freund's adjuvant and an extract of bordetella pertussis. lymphocytes from lymph nodes and spleens were equally capable of transferring disease. as few as 5 x 10(6) cells were able to transfer eao, which began on day 5-7 after transfer. infiltrate of lymphocytes and macr ... | 1987 | 2952288 |
| immunological and immunopathological aspects of opsin-induced uveoretinitis. | in an extension of our previous studies, experimental autoimmune uveoretinitis (eau) was induced in lewis rats by injection of very high doses of bovine opsin. the induced reaction consisted predominantly of a mild posterior retinitis. varying the amount of injected opsin between 300 and 1,000 micrograms did not influence this result, provided that the antigen was injected in freund's complete adjuvant. pathogenicity of opsin appeared to be lower than that of interphotoreceptor retinoid binding ... | 1987 | 2952558 |
| suppression of the cytotoxic t-lymphocyte response in mice by pertussis toxin. | pertussis toxin (pt), the major toxin produced by bordetella pertussis, has been reported both to enhance and to suppress immune responsiveness. these findings suggested that pt contributes to the virulence of b. pertussis through mechanisms involving immune regulation. we report that pt suppressed both the primary and the secondary cytotoxic t-lymphocyte (ctl) responses of mouse spleen cells cultured against two different allogeneic stimulator spleen cells in vitro. this suppression was depende ... | 1987 | 2960456 |
| enhancement of haematopoiesis-directed suppressor activity in mice following bordetella pertussis vaccine. | a single injection of killed whole-cell pertussis vaccine (strain 5374) into cba mice (h-2k) was shown to induce a drastic depletion of cells in the thymus accompanied by the enhancement of suppressor t-cell activity. thymus cells obtained from pertussis vaccine-treated mice were shown to inhibit the endogenous and exogenous colony formation in the spleen by haematopoietic stem cells (cfus). treatment of thymus cells with the anti-i-jk alloantiserum against a specific marker of suppressor t-cell ... | 1987 | 2961626 |
| induction, characterization, and cell transfer of autoimmune tubulointerstitial nephritis. | autoimmune tubulointerstitial nephritis (tin) was induced in lewis (lew) rats by immunization with homologous brown-norway (bn) rat renal basement membrane (rbm), complete freund's adjuvant and bordetella pertussis vaccine. the bn strain has a tubular basement membrane (tbm) antigen (ag+) detectable by immunofluorescence which is lacking in unmodified lew rat tbm. development of tin in lew rats correlated with tbm ag+ immunogens from homologous and heterologous rbm preparations. by day 14 after ... | 1987 | 2963168 |
| comparative effects of cholera and bordetella pertussis toxins on cyclic amp and gtp levels and on lipolysis in rat adipocytes incubated in vitro. | the respective effects of cholera and bordetella pertussis toxins were studied in time and concentration dependent experiments, following glycerol and fatty acid release, gtp and camp levels. cholera toxin, after a lag time of 30 min, stimulated linearly gtp and camp accumulation and lipolysis (maximal effect: 2-fold increase at 5 micrograms/ml). pertussis toxin presented a biphasic effect both in time and concentration dependent studies. up to a maximum reached after 2 h with 1.4 units lpf/ml t ... | 1985 | 2982667 |
| pertussis toxin facilitates the progesterone-induced maturation of xenopus oocyte. possible role of protein phosphorylation. | progesterone triggers the first meiotic cell division of xenopus oocyte and inhibits camp synthesis. the effect of pertussis toxin purified from bordetella pertussis was tested on the maturation of xenopus oocyte. the toxin did not inhibit progesterone-induced resumption of meiosis or the hormone-induced drop in camp level. this indicates that progesterone action is not mediated by the ni subunit of the oocyte adenylate cyclase. furthermore, pertussis toxin caused a reduction in the time course ... | 1985 | 2982668 |
| resistance to adenovirus infection after administration of bordetella pertussis vaccine in mice. | treatment of mice with bordetella pertussis vaccine rendered mice resistant to mouse adenovirus infection. the resistant state took at least 5 days to develop, and susceptibility returned to a portion of the test population 35 days after treatment. transient resistance developed in congenitally athymic mice also. treatment with a dose of 25 micrograms (dry weight) of b. pertussis vaccine protected approximately 50% of the test population. vaccines prepared from several different strains of b. pe ... | 1985 | 2982736 |
| potential role for a guanine nucleotide regulatory protein in chemoattractant receptor mediated polyphosphoinositide metabolism, ca++ mobilization and cellular responses by leukocytes. | islet activating protein from bordetella pertussis toxin which ribosylates certain guanine nucleotide regulatory proteins causes a marked reduction of chemoattractant-elicited responses such as chemotaxis, o2 production and camp elevations in human polymorphonuclear leukocytes. the toxin appears to exert its effects by preventing the rapid breakdown of phosphatidylinositol 4,5-bisphosphate induced by the chemotactic peptide n-formyl-methionyl-leucyl-phenylalanine, thereby inhibiting the increase ... | 1985 | 2983721 |
| solubilization from rat pancreatic plasma membranes of a cholecystokinin (cck) agonist-receptor complex interacting with guanine nucleotide regulatory proteins coexisting in the same macromolecular system. | using the non-denaturing detergent 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propane sulfonate (chaps), cholecystokinin (cck) receptors were solubilized from rat pancreatic membranes as a reversible complex with the cck 31-39 nonapeptide 125i-labelled by the bolton and hunter reagent. bound ligand dissociation from this soluble complex was similar to that from the membranous receptors of origin and the marked increase in the rate of dissociation induced by gtp was preserved in the solub ... | 1985 | 2983985 |
| chemoattractant receptor-induced hydrolysis of phosphatidylinositol 4,5-bisphosphate in human polymorphonuclear leukocyte membranes. requirement for a guanine nucleotide regulatory protein. | incubation of plasma membranes from human polymorphonuclear leukocytes (pmns) with [gamma-32p]atp in the presence of mgcl2 resulted in the formation of 32p-labeled phosphatidic acid (pa), phosphatidylinositol 4-phosphate (pip), and phosphatidylinositol 4,5-bisphosphate (pip2). membranes from pmn specific and azurophil granules synthesized only pip, suggesting that pip2 metabolism is confined to the plasma membrane in pmns. further incubations of the labeled plasma membranes for 60 s in the prese ... | 1985 | 2987206 |
| role of ni in coupling angiotensin receptors to inhibition of adenylate cyclase in hepatocytes. | angiotensin ii can inhibit glucagon-stimulated cyclic amp production in hepatocytes and adenylate cyclase activity in hepatic membranes. pertussis toxin, an exotoxin produced by bordetella pertussis, was used to investigate the role of the inhibitory guanine nucleotide-binding regulatory protein of adenylate cyclase (ni) in coupling angiotensin receptors to the adenylate cyclase system. an assay was developed using [32p] nad+ to quantitate the amount of ni protein in the membrane and the extent ... | 1985 | 2999149 |
| effect of bordetella pertussis vaccine on bleeding time, whole blood coagulation time and platelet aggregation in animals. | bordetella pertussis vaccine, a hypoglycemic agent significantly prolonged the bleeding time and whole blood coaguistion time in rabbits and dogs when administered intraperitonially. the vaccine also affects platelet aggregation in rats 4 days after its intraperitonial administration. the effect on platelet aggregation is thought to be due to its beta adrenargic receptor blocking action which may also contribute to the hypoglycemia. | 1985 | 3003424 |
| angiotensin ii and dopamine modulate both camp and inositol phosphate productions in anterior pituitary cells. involvement in prolactin secretion. | despite their opposite effects on prolactin secretion, both dopamine and angiotensin ii inhibit adenylate cyclase activity in homogenates of anterior pituitary cells in primary culture. dopamine and angiotensin ii inhibition of adenylate cyclase was not additive, suggesting that both neurohormones inhibit the adenylate cyclase of the lactotroph cells. pretreatment with bordetella pertussis toxin (islet activator protein) completely suppressed the dopamine-induced inhibition of both adenylate cyc ... | 1986 | 3005316 |
| the influence of bordetella pertussis and its constituents on the beta-adrenergic receptor in the guinea pig respiratory system. | in the present study, the effect of vaccination of guinea pigs with bordetella pertussis was investigated, 4 days after treatment, on the cholinergic and beta-adrenergic receptor function in isolated tracheal spirals and the number of beta-adrenoceptor binding sites in guinea pig lung. it was found that b. pertussis caused an impairment in the beta-adrenoceptor function and a decrease in its number. similar results were obtained with endotoxin. leucocytosis promoting factor, however, was ineffec ... | 1986 | 3005792 |
| effect of islet-activating pertussis toxin on the binding characteristics of ca2+-mobilizing hormones and on agonist activation of phosphorylase in hepatocytes. | islet-activating protein (iap, a bordetella pertussis toxin) was employed to test the hypothesis that the inhibitory gtp-binding regulatory protein of adenylate cyclase (ni) mediates gtp effects on the binding of ca2+-mobilizing hormones to liver plasma membranes and is involved in calcium mobilization stimulated by these agonists. iap added to normal liver plasma membranes catalyzed the incorporation of radioactivity from [32p]nad into a 41,000-da peptide (presumably the alpha-subunit of ni). h ... | 1986 | 3005828 |
| [stimulation of pituitary-adrenocortical system by cepharanthine]. | we observed that cepharanthine might exert its anti-allergic action by stimulating the secretion of corticosterone. the present experiments were carried out to investigate stimulation of the pituitary-adrenocortical system by cepharanthine. administration of cepharanthine to rats produced increases in plasma and adrenal corticosterone levels. administration of cepharanthine to propranolol pretreated rats also produced increases in plasma and adrenal corticosterone levels and plasma acth level. t ... | 1986 | 3009288 |
| bordetella heat-labile toxin: further purification, characterization and mode of action. | the heat-labile toxin (htl) was purified from sonic extracts of bordetella bronchiseptica and bordetella pertussis cells by a series of hydrophobic interactions, density gradient centrifugation, gel filtration, isoelectric precipitation, and isoelectric focusing. a 114-fold purification was regularly obtained with a yield of 31%. a dose of 0.75 ng was dermonecrotizing in guinea pigs. hlt is a simple protein (pi 6.9) with a molecular weight by gel filtration of 102,000 which consists of two polyp ... | 1985 | 3011569 |
| a guanine nucleotide regulatory protein controls polyphosphoinositide metabolism, ca2+ mobilization, and cellular responses to chemoattractants in human monocytes. | previous studies demonstrated that oligopeptide chemoattractant receptors on pmn and macrophages exist in high and low affinity states which are interconvertible by guanosine di- and triphosphates. these observations suggest that guanine nucleotide regulatory (n) proteins play a role in phagocyte activation by chemotactic factors. the data presented here indicate that chemotactic factor receptors on monocytes utilize an n protein to activate phospholipase c and subsequent biologic responses by t ... | 1986 | 3011906 |
| [action of plant extracts on the natural immunity indices of animals]. | the influence of extracts from oak bark, st. john's-wort leaves and pine buds on natural immunity characteristics of mice has been studied. the injection of these extracts into mice has been found to enhance their resistance to infection with staphylococcus aureus and bordetella pertussis virulent cultures, to decrease the enzymatic activity of 5'-nucleotidase in the peritoneal exudate macrophages of mice and to increase the level of lysozyme in their blood. the action of these extracts has prov ... | 1986 | 3012909 |
| activation of muscarinic receptors in pc12 cells. correlation between cytosolic ca2+ rise and phosphoinositide hydrolysis. | the intracellular signals generated by carbachol activation of the muscarinic receptor [release of inositol phosphates as a consequence of phosphoinositide hydrolysis and rise of the cytosolic ca2+ concentration ([ca2+]i, measured by quin2)] were studied in intact pc12 pheochromocytoma cells that had been differentiated by treatment with nerve growth factor. when measured in parallel samples of the same cell preparation 30 s after receptor activation, the release of inositol trisphosphate and of ... | 1986 | 3013159 |
| prokaryotic adenylate cyclase toxin stimulates anterior pituitary cells in culture. | bordetella pertussis synthesizes a variety of virulence factors including a calmodulin-dependent adenylate cyclase (ac) toxin. treatment of anterior pituitary cells with this ac toxin resulted in an increase in cellular camp levels that was associated with accelerated exocytosis of growth hormone (gh), prolactin, adrenocorticotropic hormone (acth), and luteinizing hormone (lh). the kinetics of release of these hormones, however, were markedly different; gh and prolactin were rapidly released, wh ... | 1986 | 3017120 |
| the action of islet activating protein (pertussis toxin) on insulin's ability to inhibit adenylate cyclase and activate cyclic amp phosphodiesterases in hepatocytes. | treatment of hepatocytes with islet activating protein (pertussis toxin) from bordetella pertussis blocked the ability of insulin to inhibit adenylate cyclase activity both in broken plasma membranes and in intact hepatocytes. such treatment of intact hepatocytes with pertussis toxin did not prevent insulin from activating the peripheral plasma membrane cyclic amp phosphodiesterase although it did inhibit the ability of insulin to activate the 'dense-vesicle' cyclic amp phosphodiesterase. the ab ... | 1986 | 3017298 |
| leukotriene b4 induces formation of inositol phosphates in rat peritoneal polymorphonuclear leukocytes. | leukotriene b4 (ltb4) induced rapid breakdown of prelabeled inositol phospholipids in rat peritoneal polymorphonuclear leukocytes (pmns). formation of [3h]inositol triphosphate ([3h]ip3) was rapid, with a peak of 250-300% of the control level, after 5-15 sec of exposure to ltb4. accumulation of [3h]inositol bisphosphate was rapid, peaking after 30 sec of treatment. accumulation of [3h]inositol monophosphate was also rapid in the presence of licl. the kinetics of [3h]ip3, [3h]inositol bisphosphat ... | 1986 | 3018480 |
| adenylate cyclase inhibition is not involved in the adrenal steroidogenic response to angiotensin ii. | angiotensin ii (aii) receptors in adrenal glomerulosa cells are coupled to adenylate cyclase inhibition. we investigated the importance of cyclase inhibition in adrenal steroidogenesis by treating adrenal glomerulosa cells with the toxin of bordetella pertussis (20 ng/ml) for 3 and 18 h. this treatment prevented inhibition of forskolin-stimulated adenylate cyclase by aii. however, the aldosterone response to aii was not altered by toxin treatment. these results strongly suggest that adenylate cy ... | 1986 | 3019640 |
| polymyxin-b inhibition of lps-induced interleukin-1 secretion by human monocytes is dependent upon the lps origin. | polymyxin-b (pmb) is an antibiotic known to inhibit various biological activities induced by lipopolysaccharides (lps). we have investigated the ability of pmb to inhibit lps-induced interleukin-1 (il-1) secretion by human monocytes in vitro. interleukin-1 was assayed by the conventional comitogenic assay using mice thymocytes. our data demonstrate that pmb (1-2 micrograms/assay)-mediated inhibition of lps-induced il-1 secretion depends on the origin of the lps. interleukin-1 secretions induced ... | 1986 | 3023974 |
| neurotoxicity of bordetella pertussis. | pertussis is a unique disease in which the harmful effects are mediated by an exotoxin that effects stimulation of the adrenergic system which is neuronally controlled. the interdependence of the growth of bacteria and toxin production, and the local colonization of the bacteria that precedes the clinical symptom of the disease reflect the nature of the disease. pertussis toxin enzymatically alters the function of numerous regulatory cells that is demonstrable, after an interval of time, by a sp ... | 1986 | 3024080 |
| cytoplasmic ph regulation in activated human neutrophils: effects of adenosine and pertussis toxin on na+/h+ exchange and metabolic acidification. | when stimulated, neutrophils undergo a complex change in cytoplasmic ph (phi): an incipient acidification, followed by an alkalinization which is due to activation of na+/h+ exchange. when the latter is inhibited by amiloride or by removal of extracellular na+, the actual magnitude of the initial acidification can be fully appreciated. the acidification is thought to be of metabolic origin, but the precise origin of the h+ (equivalents) remains undefined. we used adenosine, a modulator of neutro ... | 1986 | 3024727 |
| sensitivity of adipocyte lipolysis to stimulatory and inhibitory agonists in hypothyroidism and starvation. | the responsiveness of lipolysis to the stimulatory agonists noradrenaline, corticotropin and glucagon and to the inhibitory agonists n6-phenylisopropyladenosine, prostaglandin e1 and nicotinic acid was investigated with rat white adipocytes incubated with a high concentration of adenosine deaminase (1 unit/ml). the cells were obtained from fed or 48 h-starved euthyroid animals or from fed or starved animals rendered hypothyroid by 4 weeks of treatment with low-iodine diet and propylthiouracil. h ... | 1986 | 3026350 |
| antiviral activity of bordetella pertussis vaccine-elicited peritoneal exudate cells. | peritoneal exudate cells collected from mice 7 days after treatment with bordetella pertussis vaccine exhibited significant in vitro antiviral activity against vesicular stomatitis virus (vsv). vaccine-induced peritoneal exudate cells exhibited both intrinsic and extrinsic antiviral activity in culture with target vsv-infected l cells. virus replication was poor in the vaccine-induced exudate cells. coculture of vaccine-induced exudate cells and vsv-infected l cell targets decreased virus yield. ... | 1986 | 3027186 |
| leukotriene b4 stimulation of phagocytes results in the formation of inositol 1,4,5-trisphosphate. a second messenger for ca2+ mobilization. | inositol trisphosphate (insp3) production and cytosolic free ca2+ ([ca2+]i) elevations induced by leukotriene b4 (ltb4)-receptor activation were studied in the human promyelocytic-leukaemia cell line hl60, induced to differentiate by retinoic acid. the myeloid-differentiated hl60 cells respond to ltb4 by raising their [ca2+]i with a dose-response relationship similar to that shown by normal human neutrophils. the observations of the ltb4 transduction mechanism were compared with those of the tra ... | 1986 | 3028373 |
| structure-activity analysis of the activation of pertussis toxin. | bordetella pertussis, the causative agent of whooping cough, releases pertussis toxin in an inactive form. the toxin consists of an a protomer containing one s1 peptide subunit and a b oligomer containing several other peptide subunits. the toxin binds to cells via the b oligomer, and the s1 subunit is activated and expresses adp-ribosyltransferase and nad glycohydrolase activities. treatment of purified toxin with dithiothreitol (dtt) in vitro increases both activities. atp and the detergent 3- ... | 1987 | 3030399 |
| two growth factor signalling pathways in fibroblasts distinguished by pertussis toxin. | the primary action of a family of mitogens including bombesin, bradykinin, vasopressin and alpha-thrombin is to activate the hydrolysis of polyphosphoinositides. hydrolysis of phosphatidylinositol 4,5-bisphosphate (ptdins(4,5)p2) by phospholipase c is mediated through coupling of surface receptors to a gtp-binding protein (gp protein) which, in some cells, is inactivated by the toxin of bordetella pertussis. it is not known whether this signalling pathway is involved in initiating dna replicatio ... | 1987 | 3033510 |
| role of the adrenal medulla in stress-induced hyperinsulinaemia in normal mice and in mice infected with bordetella pertussis or treated with pertussis toxin. | intranasal infection of mice with a sublethal dose of bordetella pertussis or the intravenous administration of purified pertussis toxin resulted in a marked increase in the serum immunoreactive insulin concentration following ether stress. this stress-induced hyperinsulinaemia was not modified significantly by blockade of alpha 2-adrenoceptors with idazoxan, beta-adrenoceptors with propranolol, autonomic ganglia with hexamethonium, opioid receptors with naloxone, muscarinic cholinoceptors with ... | 1988 | 3045244 |
| [microbiological, pharmacokinetic and clinical studies of rokitamycin dry syrup in the pediatric field]. | rokitamycin (rkm), a newly developed macrolide antibiotic with a 16-membered ring, dissolves well under acidic conditions. it has been improved over other macrolides to minimize individual variations in its absorbability. we measured, using the ga-test, variations in gastric acidities of 43 children with ages between 1 to 14 years, and investigated the relationship between gastric acidities and pharmacokinetic values. also activities (expressed in mics) of antimicrobial agents were studied again ... | 1988 | 3050186 |
| the clinical use of macrolides. | macrolides are active against streptococcus pneumoniae, legionella spp. and mycoplasma pneumoniae, the main causes of community-acquired pneumonia they may therefore be used for the empirical treatment of community-acquired pneumonia, although emergent resistance in str. pneumoniae limits their use in some parts of the world. in patients with bronchitis the use of macrolides reduces the severity and duration of symptoms. macrolides have also been used successfully in the treatment of otitis medi ... | 1988 | 3053568 |
| selection of an erythromycin for the treatment of pertussis. | pertussis is one of the most communicable diseases of the respiratory tract and the incidence of this disease has increased substantially in recent years. bordetella pertussis is the major pathogen implicated and erythromycin is considered the drug of choice. because more studies have reported bacteriological and clinical relapses with ethylsuccinate and stearate formulations than with the estolate preparation, erythromycin estolate 50 mg/kg/d in divided doses over a 14-day period is recommended ... | 1988 | 3069427 |
| alpha-subunits of ns are released from the plasma membrane following cholera toxin activation. | cholera toxin (ct) and islet-activating protein (iap, a bordetella pertussis toxin) were employed to test the hypothesis that gtp-binding regulatory proteins are released from plasma membranes to a greater extent when 'activated' than when 'inactivated'. ct, which activates ns (the stimulatory gtp-binding regulatory protein of the adenylate cyclase system), catalyzed the incorporation of radioactivity from [32p]nad into 45 and 47.5 kda peptides associated with rat liver plasma membranes. followi ... | 1986 | 3086121 |
| dual effects of pertussis toxin on lymphoid cells in culture. | pertussis toxin (ptx), a component of bordetella pertussis, is responsible for many of the biological activities of this bacterium, including its potent adjuvant capacity. in attempt to better understand the ptx activity on the immune response in vivo, we have examined the effect of ptx on certain lymphoid cell responses in vitro which could be targets for the adjuvant activity of this molecule. ptx was found to stimulate a variety of cell responses which include (a) increased production and rel ... | 1986 | 3091265 |
| pertussigen enhances antigen-driven interferon-gamma production by sensitized lymphoid cells. | we have studied the effects on interferon-gamma (ifn-gamma) production of pertussigen, a protein toxin from bordetella pertussis that augments and prolongs delayed-type hypersensitivity (dth) reactions. lymphoid cell suspensions from immunized mice were incubated with antigen or mitogen, and the culture supernatants were assayed for ifn-gamma. the production of ifn-gamma on exposure to specific antigen or concanavalin a was greatly enhanced if mice were given pertussigen at the time of immunizat ... | 1986 | 3091271 |
| the effects of different inactivating agents on the potency, toxicity and stability of pertussis vaccine. | the effect of heat (56 degrees c for 10 min), formaldehyde (0.1% at 37 degrees for 24h), glutaraldehyde (0.05% at room temperature for 10 min), thimerosal (0.02% at 37 degrees c for 24h), acetone-i (three treatments at room temperature) and acetone-ii (three treatments at room temperature and fourth treatment at 37 degrees c), when used as inactivating agents in the preparation of pertussis suspension, was studied with regard to potency, toxicity and stability. five batches each of bordetella pe ... | 1987 | 3104344 |
| presence of three pertussis toxin substrates and go alpha immunoreactivity in both plasma and granule membranes of chromaffin cells. | gtp-binding proteins have been proposed to be involved in some secretory processes. bordetella pertussis toxin is known to catalyze adp-ribosylation of several gtp-binding proteins. in this paper, the subcellular localization of b. pertussis toxin substrates has been explored in chromaffin cells of bovine adrenal medulla. with appropriate gel electrophoresis conditions, three adp-ribosylated substrates of 39, 40 and 41 kda were detectable in both plasma and granule membranes. the more intense la ... | 1987 | 3108031 |
| mechanisms of lithium-vasopressin interaction in rabbit cortical collecting tubule. | in single cortical collecting tubules (cct) of the rabbit, guanosine 5'-triphosphate (gtp) increased the arginine vasopressin (avp)-stimulated adenylate cyclase (ac) by 60% (p less than 0.05). in contrast, guanosine 5' o-(2-thio)-diphosphate (gdp-beta s), a competitive inhibitor of gtp action on the stimulatory guanine regulatory protein (ns), reduced the avp-stimulated ac activity by 72% (p less than 0.001), indicating the presence of endogenous gtp in the cells under study. that inhibitory eff ... | 1987 | 3109254 |
| glutaraldehyde inactivated pertussis vaccine: a less histamine sensitizing vaccine. | the effects of different inactivating agents on the biological activity of the histamine sensitization factor of bordetella pertussis toxin were examined. the agents were used for inactivation in the preparation of whole cell pertussis suspension. the histamine sensitizing activity was reduced to 36.9-13.3% by treatment with glutaraldehyde, to about 50% by treatment with formaldehyde and by the acetone-ii treatment, relative to the reduction by heat treatment. treatment with thimerosal and the a ... | 1987 | 3110164 |
| g-proteins in torpedo marmorata electric organ. differential distribution in pre- and post-synaptic membranes and synaptic vesicles. | the nature of the g-proteins present in the pre- and post-synaptic plasma membranes and in the synaptic vesicles of cholinergic nerve terminals purified from the torpedo electric organ was investigated. in pre- and post-synaptic plasma membranes, bordetella pertussis toxin, known to catalyze the adp-ribosylation of the alpha-subunit of several g-proteins, labels two substrates at 41 and 39 kda. the 39 kda subunit detected by adp-ribosylation in the synaptic plasma membrane fractions was immunolo ... | 1987 | 3115830 |
| strain difference in an allergic asthma model in rats. | a new rat asthma model was devised, and with the model, allergic bronchoconstrictor responses and effects of disodium cromoglycate (dscg) were compared among wistar, lewis and fischer 344 rats. rats were actively sensitized with dnp-ascaris antigen (dnp-as) and killed bordetella pertussis vaccine. after eight days, asthmatic response was provoked by inhalation of dnp-as. the bronchomotor response was measured with a modified konzett-rössler method in diaphragm-sectioned rats. the inhalation of d ... | 1987 | 3119903 |
| chromatographic resolution and immunologic identification of the alpha 40 and alpha 41 subunits of guanine nucleotide-binding regulatory proteins from bovine brain. | a guanine nucleotide-binding regulatory protein (g protein), with subunits designated as alpha 40 beta gamma, was identified and partially resolved from two other purified g proteins, go (alpha 39 beta gamma) and gi (alpha 41 beta gamma), found in bovine brain. the alpha 40 g protein subunit served as a substrate for adp-ribosylation catalyzed by bordetella pertussis toxin, as did alpha 39 and alpha 41. alpha 40 was shown to be closely related to, but distinct from, alpha 41 by reaction with var ... | 1988 | 3123484 |
| inhibition of adenylate cyclase from luteinized rat ovary by monovalent cations: roles of the stimulatory guanine nucleotide-binding regulatory component and stimulatory hormone receptor. | sodium and other monovalent cations (added as chloride salts) inhibited adenylate cyclase of luteinized rat ovary. sodium chloride (150 mm) inhibited basal enzyme activity by 20%. sodium chloride inhibition was enhanced to 34-54% under conditions of enzyme stimulation by guanine nucleotides (gtp and its nonhydrolyzable analog 5'-guanylyl imidodiphosphate), fluoride anion, and agonists (ovine luteinizing hormone (olh) and the beta-adrenergic catecholamine isoproterenol) acting at stimulatory rece ... | 1988 | 3124764 |
| enhancement of experimental allergic encephalomyelitis in mice by antibodies against ifn-gamma. | acute experimental allergic encephalomyelitis (eae) was induced in c57bl/6j and sjl/j mice by injection of isologous spinal cord homogenate given in conjunction with bordetella pertussis and freund's adjuvant. sjl/j mice showed a highly aggressive and 100% lethal form of the disease; c57bl/6j mice were much less susceptible as they had low morbidity rates (20 to 40%), low disease scores, and mostly no mortality. treatment of these low susceptibility mice with neutralizing mab against ifn-gamma c ... | 1988 | 3126227 |
| tumour necrosis factor (cachectin) induces phospholipase a2 activity and synthesis of a phospholipase a2-activating protein in endothelial cells. | tumour necrosis factor (tnf) is an important mediator of endotoxin-induced vascular collapse and other inflammatory reactions. eicosanoids have been implicated in the pathogeensis of these responses. in order to explore further the potential interactions between tnf and eicosanoid metabolism in eliciting vascular responses, we studied the effects of tnf on the bovine endothelial cell line cpae. tnf induced cellular retraction observed by light microscope. this morphological change was monitored ... | 1988 | 3128274 |
| the influence of adjuvant on induction of protective immunity by a non-living vaccine against schistosomiasis. | mice were protected against subsequent infection with schistosoma mansoni by intradermal or s.c. vaccination with killed schistosomula or soluble parasite extracts and bacillus calmette-guérin (bcg). treatment with i.p. immunization was somewhat less effective, whereas i.m. vaccination failed to elicit protective immunity. the level of resistance induced by intradermal immunization was influenced by the strain of bcg used, and isolated bcg cell walls did not reliably substitute for whole bcg org ... | 1988 | 3128608 |
| endogenous tumor necrosis factor induction with bordetella pertussis vaccine as a triggering agent and its therapeutic effect on mm46 carcinoma-bearing mice. | induction of endogenous tumor necrosis factor (tnf) by administration of bordetella pertussis vaccine (bpv) as a triggering agent and its therapeutic effect against mm46 carcinoma were investigated in c3h/he mice. test triggering agents were injected intravenously into mice after intravenous injection of 4-fold dilution of macrophage activating factor (maf) or 10(4) units of murine interferon-gamma (mu-ifn-gamma). then sera were obtained from the mice, and their tnf activities were assayed on l- ... | 1988 | 3131286 |
| multiple species and isoforms of bordetella pertussis toxin substrates. | in purified g proteins from bovine brain cortex the adp-ribosylated substrates of bordetella pertussis toxin (pt) can be resolved in three polypeptides by polyacrylamide gel electrophoresis: a 39 kda major substrate, corresponding to go alpha and two others (40 and 41 kda) assigned to alpha subunits of gi-like proteins. these three polypeptides were also detected in membranes of normal cells or tissues from neuronal and endocrine origins. in contrast, in membranes from other origins, only two pt ... | 1988 | 3132153 |
| evidence that muscarinic receptors in islet cells are not coupled functionally to adenylate cyclase through the inhibitory guanine nucleotide binding protein (ni). | the effect of muscarinic agonist on adenylate cyclase was investigated in neonatal islet cells and in a clonal pituitary cell line (gh4c1) following labelling of the intracellular atp pool with [2,8 3h]adenine. in islet cells carbamylcholine was without effect on basal or glucagon-stimulated adenylate cyclase activity, measured as 3h cyclic amp production, but inhibited 3h cyclic amp production in the clonal pituitary cells. the involvement of the inhibitory guanine nucleotide binding protein of ... | 1988 | 3133298 |
| adp-ribosyltransferase mutations in the catalytic s-1 subunit of pertussis toxin. | the adp-ribosyltransferase activity of pertussis toxin resides within the s-1 subunit of the toxin. deletion mapping of a recombinant s-1 subunit produced in escherichia coli showed that amino acids 2 through 180 are required for adp-ribosylation of gi protein. mutants of the s-1 subunit which lacked either amino acids 2 through 22 or amino acids 153 through 180 failed to express enzyme activity, implicating a functional or structural role for these residues in catalysis. the catalytic carboxy-t ... | 1988 | 3135265 |
| endotoxin-induced tumor necrosis factor (tnf): selective triggering of tnf and interleukin-1 production by distinct glucosamine-derived lipids. | the isolated lipid a of bordetella pertussis endotoxin (lipa) has been found to induce in vitro release of tumor necrosis factor (tnf) by murine macrophages, albeit much less efficiently than does the intact lipopolysaccharide. synthetic analogs (monosaccharides m4 and m6) of both glucosamine units present in the lipa backbone induced production of tnf by peritoneal macrophages of swiss mice. macrophages from a/j mice gave higher responses than those from swiss mice, while those of c3h/hej mice ... | 1988 | 3135943 |
| alteration of non-specific resistance to infection with listeria monocytogenes. | the experimental infection of murine hosts with listeria monocytogenes is often used as a model for cell-mediated immunity. however, the natural immunity or non-specific resistance to listeriosis can be influenced by the parasite itself and also by a wide array of endogenous and exogenous host factors. the most important host factor in inbred mouse strains is their genetically determined susceptibility or resistance to listeria monocytogenes. secondly, the age of the mice is crucial for the outc ... | 1988 | 3138182 |
| guanosine 5'-triphosphate binding protein (gi) and two additional pertussis toxin substrates associated with muscarinic receptors in rat heart myocytes: characterization and age dependency. | the coupling of muscarinic receptors with g-proteins was investigated in cultured myocytes prepared from the hearts of newborn rats. the coupling was investigated in both young (5 days after plating) and aged (14 days after plating) cultures, in view of the completely different effects of 5'-guanylyl imidodiphosphate [gpp(nh)p] on muscarinic agonist binding to homogenates from young vs aged cultures [moscona-amir, e., henis, y. i., yechiel, e., barenholz, y., & sokolovsky, m. (1986) biochemistry ... | 1988 | 3139031 |
| g-proteins in skeletal muscle. evidence for a 40 kda pertussis-toxin substrate in purified transverse tubules. | in muscle, it has been established that guanosine 5'-[gamma-thio]triphosphate (gtp[s]), a non-hydrolysable gtp analogue, elicits a rise in tension in chemically skinned fibres, and that pretreatment with bordetella pertussis toxin (ptx) decreases gtp[s]-induced tension development [di virgilio, salviati, pozzan & volpe (1986) embo j. 5, 259-262]. in the present study, g-proteins were analysed by ptx-catalysed adp-ribosylation and by immunoblotting experiments at cellular and subcellular levels. ... | 1988 | 3140802 |
| nucleotide sequence of the bacillus anthracis edema factor gene (cya): a calmodulin-dependent adenylate cyclase. | the nucleotide sequence of the bacillus anthracis edema factor (ef) gene (cya), which encodes a calmodulin-dependent adenylate cyclase, has been determined. ef is part of the tripartite protein exotoxin of b. anthracis. an atg start codon, immediately upstream from codons which specify the first 15 amino acids (aa) of ef, was preceded by an aaaggaggt sequence which is its probable ribosome-binding site. starting at this atg codon, there was a continuous 2400-bp open reading frame which encodes t ... | 1988 | 3149607 |
| production of tumor necrosis factor in nude mice by muramyl peptides associated with bacterial vaccines. | gram-negative vaccines can elicit the production of tumor necrosis factor (tnf) in mice primed by muramyl dipeptide (mdp) or by its lipophilic derivative mdp-dipalmitoyl glycerol (mdp-gdp). in mice pretreated with mdp and particularly with mdp-gdp, bordetella pertussis vaccine was shown to be more effective than typhoid vaccine. the time course of tnf production in the blood did not indicate any difference between the effect of mdp or of mdp-gdp. in both cases the cytotoxic activity reached maxi ... | 1988 | 3167834 |
| experimental respiratory infection with bordetella pertussis in mice: comparison of two methods. | the mouse respiratory model is being used increasingly to study the pathogenesis and immunology of bordetella pertussis infection. two methods of inoculation, aerosol and intranasal, are routinely used to establish the infection. we compared the two methods of inoculation for reproducibility of infection using quantitative lung cultures and distribution of infection with [35s] methionine labeled bacteria and pulmonary histopathology. ability to produce a respiratory infection intranasally was re ... | 1988 | 3168352 |
| the comparative in-vitro activity of ofloxacin. | the antibacterial activity of ofloxacin, a new fluoroquinolone, was evaluated against a wide range of clinical bacterial isolates and compared with that of nalidixic acid, norfloxacin, enoxacin, pefloxacin and ciprofloxacin by determination of minimum inhibitory concentrations (mics). ofloxacin was very active against nalidixic acid-susceptible isolates of the enterobacteriaceae (mic less than or equal to 0.12 mg/l) and was also active against strains resistant to nalidixic acid (mic less than o ... | 1988 | 3182468 |
| adp-ribosylation of bovine s-antigen by cholera toxin. | the s-antigen (alias 48k protein or arrestin) of bovine rod photoreceptors contains two stretches of amino acid sequence homologous to the adp-ribosylation sites of the alpha subunit of transducin (ta). we have found that cholera toxin transfers the adp-ribosyl group from nad to purified bovine s-antigen as well as to s-antigen in rod outer segment membranes, while bordetella pertussis toxin is unable to catalyze the transfer reaction efficiently. under the same conditions, both toxins catalyzed ... | 1988 | 3190695 |
| the effects of purified pertussis components and lipopolysaccharide on the results of the mouse weight gain test. | the effects of highly purified components of bordetella pertussis, that is pertussis toxin (pt) and filamentous haemagglutinin (fha), and of lipopolysaccharide (lps) were studied in the active mouse weight gain test (mwgt). the pt when given alone or with other components in various combinations caused weight losses and deaths 2-3 days after inoculation but fha was not toxic in the mwgt. when fha was given with pt, the toxic effect of pt was reduced. the lps caused weight losses at 24 h which de ... | 1988 | 3198661 |
| cck and gastrin inhibit adenylate cyclase activity through a pertussis toxin-sensitive mechanism in the tumoral rat pancreatic acinar cell line ar 4-2j. | (thr28,nle31)cck(23-33) (cck-9) and gastrin(1-17)i (gastrin) inhibited adenylate cyclase activity in membranes from the tumoral rat pancreatic acinar cell line ar 4-2j through a bordetella pertussis toxin-sensitive mechanism. this contrasted with the stimulatory effect exerted by cck-9 on adenylate cyclase activity in membranes from normal rat pancreas. the relative potency of cck-9, gastrin, and related peptides in inhibiting adenylate cyclase, when confronted with previous evidence, suggests t ... | 1988 | 3203744 |
| intraperitoneal injection of bordetella pertussis vaccine and thiomersal failed to produce intussusception in mice. | | 1988 | 3227729 |
| elevation of glucose-6-phosphate dehydrogenase levels in alloxan diabetic mice by bordetella pertussis cell extract. | | 1988 | 3243559 |