| pilf is an outer membrane lipoprotein required for multimerization and localization of the pseudomonas aeruginosa type iv pilus secretin. | type iv pili (t4p) are retractile appendages that contribute to the virulence of bacterial pathogens. pilf is a pseudomonas aeruginosa lipoprotein that is essential for t4p biogenesis. phenotypic characterization of a pilf mutant confirmed that t4p-mediated functions are abrogated: t4p were no longer present on the cell surface, twitching motility was abolished, and the mutant was resistant to infection by t4p retraction-dependent bacteriophage. the results of cellular fractionation studies indi ... | 2008 | 18776008 |
| effective mutagenesis of vibrio fischeri by using hyperactive mini-tn5 derivatives. | we have developed a transposon mutagenesis system for vibrio fischeri es114 that utilizes a hyperactive mutant tn5 transposase (e54k and m56a) and optimized transposon ends. using a conjugation-based procedure, we obtained independent single-insertion mini-tn5 mutants at a rate of approximately 10(-6). this simple and inexpensive technique represents a significant improvement over previous methods for transposon mutagenesis of v. fischeri and should be applicable to many other bacteria. | 2008 | 18805998 |
| acclimated biomass that degrades sulfonated naphthalene formaldehyde condensate. | a number of aerobic species were isolated from textile industry activated sludge wastewater. the bacterial consortium was acclimated during seven days before testing its capacity of sulfonated naphthalene-formaldehyde condensate (snfc) recalcitrant compound degradation. snfc's degradation was evaluated by using different techniques including: vapour pressure osmometry, spectroscopy uv-visible and chemical oxygen demand (cod). the degradation of snfc by acclimated bacterial consortium was determi ... | 2008 | 18819646 |
| nitric oxide reductase gene expression and nitrous oxide production in nitrate-grown pseudomonas mandelii. | pure cultures of pseudomonas mandelii were incubated with or without nitrate, which acts as a substrate and an electron acceptor for denitrification. nitric oxide reductase (cnorb) gene expression was measured using a quantitative reverse transcription-pcr, and nitrous oxide emissions were measured by gas chromatography. p. mandelii cells in either the presence or absence of nitrate demonstrated an increase in cnorb gene expression during the first 3 h of growth. the level of expression of cnorb ... | 2008 | 18820058 |
| phylogenetic analysis and siderotyping as useful tools in the taxonomy of pseudomonas stutzeri: description of a novel genomovar. | an examination of the results of phylogenetic analyses based on the sequences of fragments of the 16s rrna, gyrb and rpod genes, and the discrimination of genomovars based on siderophore diversity within the genus pseudomonas, has added important taxonomic tools in the characterization of pseudomonas stutzeri. eighteen reference strains, nine newly identified hydrocarbon-degrading strains and three strains showing relevant physiological characteristics of p. stutzeri, together with the type stra ... | 2008 | 18842847 |
| cross-reaction of stenotrophomonas and xanthomonas species in a 23s rrna gene-directed pcr for detection of s. maltophilia. | | 2008 | 18842940 |
| toxicogenomic response of pseudomonas aeruginosa to ortho-phenylphenol. | pseudomonas aeruginosa (p. aeruginosa) is the most common opportunistic pathogen implicated in nosocomial infections and in chronic lung infections in cystic fibrosis patients. ortho-phenylphenol (opp) is an antimicrobial agent used as an active ingredient in several epa registered disinfectants. despite its widespread use, there is a paucity of information on its target molecular pathways and the cellular responses that it elucidates in bacteria in general and in p. aeruginosa in particular. an ... | 2008 | 18847467 |
| structure of l-xylulose-5-phosphate 3-epimerase (ulae) from the anaerobic l-ascorbate utilization pathway of escherichia coli: identification of a novel phosphate binding motif within a tim barrel fold. | three catabolic enzymes, ulad, ulae, and ulaf, are involved in a pathway leading to fermentation of l-ascorbate under anaerobic conditions. ulad catalyzes a beta-keto acid decarboxylation reaction to produce l-xylulose-5-phosphate, which undergoes successive epimerization reactions with ulae (l-xylulose-5-phosphate 3-epimerase) and ulaf (l-ribulose-5-phosphate 4-epimerase), yielding d-xylulose-5-phosphate, an intermediate in the pentose phosphate pathway. we describe here crystallographic studie ... | 2008 | 18849419 |
| degradation of phenanthrene by bacterial strain isolated from soil in oil refinery fields in shanghai china. | a bacterial strain pseudomonas stutzeri zp2 was identified with phenanthrene-degrading ability based on gram staining, oxydase reaction, biochemical tests, fame analysis, g+c content and 16s rdna gene sequence analysis. it is the first time that p. stutzeri is reported to process the capability for phenanthrene degradation. the strain was isolated from soil samples contaminated with polycyclic aromatic hydrocarbon (pah)-containing waste from an oil refinery field in shanghai, china. strain p sp. ... | 2009 | 18930349 |
| phosphorus deprivation responses and phosphonate utilization in a thermophilic synechococcus sp. from microbial mats. | the genomes of two closely related thermophilic cyanobacterial isolates, designated synechococcus isolate os-a and synechococcus isolate os-b', from the microbial mats of octopus spring (yellowstone national park) have been sequenced. an extensive suite of genes that are controlled by phosphate levels constitute the putative pho regulon in these cyanobacteria. we examined physiological responses of an axenic os-b' isolate as well as transcript abundances of pho regulon genes as the cells acclima ... | 2008 | 18931115 |
| resolving genetic functions within microbial populations: in situ analyses using rrna and mrna stable isotope probing coupled with single-cell raman-fluorescence in situ hybridization. | prokaryotes represent one-half of the living biomass on earth, with the vast majority remaining elusive to culture and study within the laboratory. as a result, we lack a basic understanding of the functions that many species perform in the natural world. to address this issue, we developed complementary population and single-cell stable isotope ((13)c)-linked analyses to determine microbial identity and function in situ. we demonstrated that the use of rrna/mrna stable isotope probing (sip) rec ... | 2009 | 18997025 |
| resolving genetic functions within microbial populations: in situ analyses using rrna and mrna stable isotope probing coupled with single-cell raman-fluorescence in situ hybridization. | prokaryotes represent one-half of the living biomass on earth, with the vast majority remaining elusive to culture and study within the laboratory. as a result, we lack a basic understanding of the functions that many species perform in the natural world. to address this issue, we developed complementary population and single-cell stable isotope ((13)c)-linked analyses to determine microbial identity and function in situ. we demonstrated that the use of rrna/mrna stable isotope probing (sip) rec ... | 2009 | 18997025 |
| enantioselective oxidation of racemic lactic acid to d-lactic acid and pyruvic acid by pseudomonas stutzeri sdm. | d-lactic acid and pyruvic acid are two important building block intermediates. production of d-lactic acid and pyruvic acid from racemic lactic acid by biotransformation is economically interesting. biocatalyst prepared from 9 g dry cell wt l(-1) of pseudomonas stutzeri sdm could catalyze 45.00 g l(-1)dl-lactic acid into 25.23 g l(-1)d-lactic acid and 19.70 g l(-1) pyruvic acid in 10h. using a simple ion exchange process, d-lactic acid and pyruvic acid were effectively separated from the biotran ... | 2009 | 19000943 |
| pseudomonas stutzeri associated conjunctivitis. | | 2008 | 19008607 |
| genomic islands of pseudomonas aeruginosa. | key to pseudomonas aeruginosa's ability to thrive in a diversity of niches is the presence of numerous genomic islands that confer adaptive traits upon individual strains. we reasoned that p. aeruginosa strains capable of surviving in the harsh environments of multiple hosts would therefore represent rich sources of genomic islands. to this end, we identified a strain, pse9, that was virulent in both animals and plants. subtractive hybridization was used to compare the genome of pse9 with the le ... | 2009 | 19025565 |
| genomic islands of pseudomonas aeruginosa. | key to pseudomonas aeruginosa's ability to thrive in a diversity of niches is the presence of numerous genomic islands that confer adaptive traits upon individual strains. we reasoned that p. aeruginosa strains capable of surviving in the harsh environments of multiple hosts would therefore represent rich sources of genomic islands. to this end, we identified a strain, pse9, that was virulent in both animals and plants. subtractive hybridization was used to compare the genome of pse9 with the le ... | 2009 | 19025565 |
| aerobic reduction of perchlorate by bacteria isolated in kerala, south india. | possibility of perchlorate reduction by microbes raises hope for an eco-friendly mode of degradation of this toxic rocket fuel. this study reports 3 isolates (a1, a2 and a3) capable of molybdenum-independent degradation of perchlorate under aerobic conditions. the rate of degradation was the highest when perchlorate concentration was 17 mm, and then 3.2 mm, 4.7 mm and 4.1 mm of perchlorate was reduced by isolates a1, a2 and a3 (respectively) after 72 h at 28 degrees c under aerobic conditions. p ... | 2008 | 19029691 |
| common thiolation mechanism in the biosynthesis of trna thiouridine and sulphur-containing cofactors. | 2-thioribothymidine (s(2)t), a modified uridine, is found at position 54 in transfer rnas (trnas) from several thermophiles; s(2)t stabilizes the l-shaped structure of trna and is essential for growth at higher temperatures. here, we identified an atpase (trna-two-thiouridine c, ttuc) required for the 2-thiolation of s(2)t in thermus thermophilus and examined in vitro s(2)t formation by ttuc and previously identified s(2)t-biosynthetic proteins (ttua, ttub, and cysteine desulphurases). the c-ter ... | 2008 | 19037260 |
| case of indolent endocarditis due to pseudomonas stutzeri with genetic evidence of relapse after 4 years. | pseudomonas stutzeri, a gram-negative bacterium, is a common inhabitant of soil and water. we report an unusual case of a relapse of infective endocarditis due to p. stutzeri 4 years after the initial episode. the identity of the strains was proven by genomic analysis. | 2009 | 19052175 |
| spectroscopic and computational studies of nitrite reductase: proton induced electron transfer and backbonding contributions to reactivity. | a combination of spectroscopy and dft calculations has been used to define the geometric and electronic structure of the nitrite bound type 2 (t2) copper site at high and low ph in nitrite reductase from rhodobacter sphaeroides. at high ph there is no electron transfer from reduced type 1 (t1) to the nitrite bound t2 copper, while protonation triggers t1 --> t2 electron transfer and generation of no. the dft calculated reaction coordinate for the n-o bond cleavage in nitrite reduction by the red ... | 2009 | 19053185 |
| conjugative interaction induces transposition of ispst9 in pseudomonas stutzeri an10. | ispst9 is an isl3-like insertion sequence (is) that was recently described in the naphthalene-degrading organism pseudomonas stutzeri strain an10. in this paper we describe a novel strong is regulation stimulus; transposition of ispst9 is induced in all p. stutzeri an10 cells after conjugative interaction with escherichia coli. thus, we observed that in all p. stutzeri an10 cells that received genetic material by conjugation the ispst9 genomic dose and/or distribution was changed. furthermore, i ... | 2009 | 19060139 |
| purine utilization by klebsiella oxytoca m5al: genes for ring-oxidizing and -opening enzymes. | the enterobacterium klebsiella oxytoca uses a variety of inorganic and organic nitrogen sources, including purines, nitrogen-rich compounds that are widespread in the biosphere. we have identified a 23-gene cluster that encodes the enzymes for utilizing purines as the sole nitrogen source. growth and complementation tests with insertion mutants, combined with sequence comparisons, reveal functions for the products of these genes. here, we report our characterization of 12 genes, one encoding gua ... | 2009 | 19060149 |
| purine utilization by klebsiella oxytoca m5al: genes for ring-oxidizing and -opening enzymes. | the enterobacterium klebsiella oxytoca uses a variety of inorganic and organic nitrogen sources, including purines, nitrogen-rich compounds that are widespread in the biosphere. we have identified a 23-gene cluster that encodes the enzymes for utilizing purines as the sole nitrogen source. growth and complementation tests with insertion mutants, combined with sequence comparisons, reveal functions for the products of these genes. here, we report our characterization of 12 genes, one encoding gua ... | 2009 | 19060149 |
| electrochemical evidence for multiple peroxidatic heme states of the diheme cytochrome c peroxidase of pseudomonas aeruginosa. | the enzyme cytochrome c peroxidase from pseudomonas aeruginosa and its catalytic mechanism were investigated using protein film voltammetry. monolayers of the diheme bacterial enzyme were immobilized on both pyrolytic graphite edge and alkanethiol-modified au electrodes. the redox couple associated with the low potential heme could be detected on both electrode surfaces at a reduction potential of -234 mv vs she. the midpoint potential displays a distinct ph dependence at acidic ph values, indic ... | 2009 | 19072039 |
| vectorial proton transfer coupled to reduction of o2 and no by a heme-copper oxidase. | the heme-copper oxidase (hcuo) superfamily consists of integral membrane proteins that catalyze the reduction of either oxygen or nitric oxide. the hcuos that reduce o(2) to h(2)o couple this reaction to the generation of a transmembrane proton gradient by using electrons and protons from opposite sides of the membrane and by pumping protons from inside the cell or organelle to the outside. the bacterial no-reductases (nor) reduce no to n(2)o (2no + 2e(-) + 2h(+) --> n(2)o + h(2)o), a reaction a ... | 2008 | 19074284 |
| biochemical and genetic characterization of pspe and glpe, two single-domain sulfurtransferases of escherichia coli. | the pspe and glpe genes of escherichia coli encode periplasmic and cytoplasmic single-domain rhodaneses, respectively, that catalyzes sulfur transfer from thiosulfate to thiophilic acceptors. strains deficient in either or both genes were constructed. comparison of rhodanese activity in these strains revealed that pspe provides 85% of total rhodanese activity, with glpe contributing most of the remainder. pspe activity was four times higher during growth on glycerol versus glucose, and was not i ... | 2008 | 19088907 |
| genomic sequence and activity of ks10, a transposable phage of the burkholderia cepacia complex. | the burkholderia cepacia complex (bcc) is a versatile group of gram negative organisms that can be found throughout the environment in sources such as soil, water, and plants. while bcc bacteria can be involved in beneficial interactions with plants, they are also considered opportunistic pathogens, specifically in patients with cystic fibrosis and chronic granulomatous disease. these organisms also exhibit resistance to many antibiotics, making conventional treatment often unsuccessful. ks10 wa ... | 2008 | 19094239 |
| biosynthetic gene cluster of cetoniacytone a, an unusual aminocyclitol from the endosymbiotic bacterium actinomyces sp. lu 9419. | a gene cluster responsible for the biosynthesis of the antitumor agent cetoniacytone a was identified in actinomyces sp. strain lu 9419, an endosymbiotic bacterium isolated from the intestines of the rose chafer beetle (cetonia aurata). the nucleotide sequence analysis of the 46 kb dna region revealed the presence of 31 complete orfs, including genes predicted to encode a 2-epi-5-epi-valiolone synthase (ceta), a glyoxalase/bleomycin resistance protein (cetb), an acyltransferase (cetd), an fad-de ... | 2009 | 19101977 |
| carbapenem resistance among pseudomonas aeruginosa strains from india: evidence for nationwide endemicity of multiple metallo-beta-lactamase clones (vim-2, -5, -6, and -11 and the newly characterized vim-18). | among 57 metallo-beta-lactamase (mbetal)-producing pseudomonas spp. detected in india during 2006, five bla(vim) genes were found, including a newly characterized bla(vim) gene. pseudomonas aeruginosa strains were clustered in 33 ribotypes with clones found in multiple hospitals. several types of bla(vim-2)-carrying integrons were detected. the newly characterized variant vim-18 showed a 4-amino-acid deletion compared to other vim variants. in this study, we show that vim-producing pseudomonas s ... | 2009 | 19114677 |
| carbapenem resistance among pseudomonas aeruginosa strains from india: evidence for nationwide endemicity of multiple metallo-beta-lactamase clones (vim-2, -5, -6, and -11 and the newly characterized vim-18). | among 57 metallo-beta-lactamase (mbetal)-producing pseudomonas spp. detected in india during 2006, five bla(vim) genes were found, including a newly characterized bla(vim) gene. pseudomonas aeruginosa strains were clustered in 33 ribotypes with clones found in multiple hospitals. several types of bla(vim-2)-carrying integrons were detected. the newly characterized variant vim-18 showed a 4-amino-acid deletion compared to other vim variants. in this study, we show that vim-producing pseudomonas s ... | 2009 | 19114677 |
| utility of peptide nucleic acid fluorescence in situ hybridization for rapid detection of acinetobacter spp. and pseudomonas aeruginosa. | the utility of peptide nucleic acid fluorescence in situ hybridization (pna fish) for the detection of acinetobacter spp. and pseudomonas aeruginosa was evaluated on broth suspensions and spiked blood cultures of atcc strains and clinical isolates with select gram-negative rods. after testing 60 clinical isolates, pna fish had a sensitivity and specificity of 100% and 100%, respectively, for acinetobacter spp. and 100% and 95%, respectively, for p. aeruginosa. pna fish was able to detect both pa ... | 2009 | 19116347 |
| utility of peptide nucleic acid fluorescence in situ hybridization for rapid detection of acinetobacter spp. and pseudomonas aeruginosa. | the utility of peptide nucleic acid fluorescence in situ hybridization (pna fish) for the detection of acinetobacter spp. and pseudomonas aeruginosa was evaluated on broth suspensions and spiked blood cultures of atcc strains and clinical isolates with select gram-negative rods. after testing 60 clinical isolates, pna fish had a sensitivity and specificity of 100% and 100%, respectively, for acinetobacter spp. and 100% and 95%, respectively, for p. aeruginosa. pna fish was able to detect both pa ... | 2009 | 19116347 |
| arginine racemization by coupled catabolic and anabolic dehydrogenases. | d-amino acids exist in living organisms as specialized components of many different machineries. biosynthesis of d-amino acids from racemization of predominant l-enantiomers is catalyzed by a single enzyme. here, we report the finding of a novel 2-component amino acid racemase for d-to-l inversion in d-arginine metabolism of pseudomonas aeruginosa. from dna microarray analysis, the putative daubar operon (for d-arginine utilization) of unknown functions was found to be highly induced by d-argini ... | 2009 | 19139398 |
| biocide susceptibility of the burkholderia cepacia complex. | the burkholderia cepacia complex (bcc) species are important opportunistic pathogens with intrinsic antibiotic resistance. they are also well known as contaminants of disinfectants, yet their biocide susceptibility has not been studied in detail. we investigated bcc biocide susceptibility and correlated it to their taxonomy, antibiotic susceptibility and ability to form biofilms. | 2009 | 19153076 |
| accommodation of two diatomic molecules in cytochrome bo: insights into no reductase activity in terminal oxidases. | bacterial heme-copper terminal oxidases react quickly with no to form a heme-nitrosyl complex, which, in some of these enzymes, can further react with a second no molecule to produce n(2)o. previously, we characterized the heme a(3)-no complex formed in cytochrome ba(3) from thermus thermophilus and the product of its low-temperature illumination. we showed that the photolyzed no group binds to cu(b)(i) to form an end-on no-cu(b) or a side-on copper-nitrosyl complex, which is likely to represent ... | 2009 | 19187032 |
| non-sterilized fermentative production of polymer-grade l-lactic acid by a newly isolated thermophilic strain bacillus sp. 2-6. | the demand for lactic acid has been increasing considerably because of its use as a monomer for the synthesis of polylactic acid (pla), which is a promising and environment-friendly alternative to plastics derived from petrochemicals. optically pure l-lactic acid is essential for polymerization of pla. the high fermentation cost of l-lactic acid is another limitation for pla polymers to compete with conventional plastics. | 2009 | 19194504 |
| proteomic detection of proteins involved in perchlorate and chlorate metabolism. | mass spectrometry and a time-course cell lysis method were used to study proteins involved in perchlorate and chlorate metabolism in pure bacterial cultures and environmental samples. the bacterial cultures used included dechlorosoma sp. kj, dechloromonas hortensis, pseudomonas chloritidismutans ask-1, and pseudomonas stutzeri. the environmental samples included an anaerobic sludge enrichment culture from a sewage treatment plant, a sample of a biomass-covered activated carbon matrix from a bior ... | 2009 | 19199051 |
| electron transfer patterns of the di-heme protein cytochrome c(4) from pseudomonas stutzeri. | we report kinetic data for the two-step electron transfer (et) oxidation and reduction of the two-domain di-heme redox protein pseudomonas stutzeri cytochrome (cyt) c(4) by [co(bipy)(3)](2+/3+) (bipy=2,2'-bipyridine). following earlier reports, the data accord with both bi- and tri-exponential kinetics. a complete kinetic scheme includes both "cooperative"intermolecular et between each heme group and the external reaction partner, and intramolecular et between the two heme groups. a new data ana ... | 2009 | 19217165 |
| structural analysis of ligand stimulation of the histidine kinase narx. | histidine kinase receptors are a large family of membrane-spanning proteins found in many prokaryotes and some eukaryotes. they are a part of two-component signal transduction systems, which each comprise a sensor kinase and a response regulator and are involved with the regulation of many cellular processes. narx is a histidine kinase receptor that responds to nitrate and nitrite to effect regulation of anaerobic respiration in various bacteria. we present high-resolution x-ray crystal structur ... | 2009 | 19217390 |
| species distribution and antimicrobial susceptibility of gram-negative aerobic bacteria in hospitalized cancer patients. | nosocomial infections pose significant threats to hospitalized patients, especially the immunocompromised ones, such as cancer patients. | 2009 | 19228413 |
| identification of neisserial dna binding components. | neisseria meningitidis, a causative agent of meningitis and septicaemia, expresses type iv pili, a feature correlating with the uptake of exogenous dna from the environment by natural transformation. the outer membrane complex pilq, through which pili are extruded and retracted, has previously been shown to bind dna in its pore region. in order to further elucidate how dna is transported across the membranes, we searched for dna binding proteins within the meningococcal inner membrane. inner mem ... | 2009 | 19246756 |
| active site of cytochrome cbb3. | cytochrome cbb(3) is the most distant member of the heme-copper oxidase family still retaining the following major feature typical of these enzymes: reduction of molecular oxygen to water coupled to proton translocation across the membrane. the thermodynamic properties of the six redox centers, five hemes and a copper ion, in cytochrome cbb(3) from rhodobacter sphaeroides were studied using optical and epr spectroscopy. the low spin heme b in the catalytic subunit was shown to have the highest m ... | 2009 | 19252222 |
| low rates of pseudomonas aeruginosa misidentification in isolates from cystic fibrosis patients. | pseudomonas aeruginosa is an important cause of pulmonary infection in cystic fibrosis (cf). its correct identification ensures effective patient management and infection control strategies. however, little is known about how often cf sputum isolates are falsely identified as p. aeruginosa. we used p. aeruginosa-specific duplex real-time pcr assays to determine if 2,267 p. aeruginosa sputum isolates from 561 cf patients were correctly identified by 17 australian clinical microbiology laboratorie ... | 2009 | 19261796 |
| a novel c-type lectin (cflec-3) from chlamys farreri with three carbohydrate-recognition domains. | c-type lectins are a superfamily of carbohydrate-recognition proteins which play crucial roles in the innate immunity. in this study, the gene of a c-type lectin with multiple carbohydrate-recognition domains (crds) from scallop chlamys farreri (designated as cflec-3) was cloned by rapid amplification of cdna ends (race) approach based on expression sequence tag (est) analysis. the full-length cdna of cflec-3 was of 2256 bp. the open reading frame encoded a polypeptide of 516 amino acids, includ ... | 2009 | 19264137 |
| structure of the redox sensor domain of methylococcus capsulatus (bath) mmos. | mmos from methylococcus capsulatus (bath) is the multidomain sensor protein of a two-component signaling system proposed to play a role in the copper-mediated regulation of soluble methane monooxygenase (smmo). mmos binds an fad cofactor within its n-terminal tandem per-arnt-sim (pas) domains, suggesting that it functions as a redox sensor. the crystal structure of the mmos tandem pas domains, designated pas-a and pas-b, has been determined to 2.34 a resolution. both domains adopt the typical pa ... | 2009 | 19271777 |
| biosorptive capacity of cd(ii) and cu(ii) by lyophilized cells of pseudomonas stutzeri. | the biosorptive capacity of cd(ii) and cu(ii) by lyophilized cells of pseudomonas stutzeri was investigated based on langmuir and freundlich isotherms and biosorption kinetics were analyzed using first order kinetic with different initial metal concentrations. biosorptive capacity for cd(ii) and cu(ii) decreased with an increment of metal concentration, reaching 43.5 and 36.2 mg/g at the initial concentration of 300 mg/l. biosorption capacity for both metal ions was increased with increasing ph. ... | 2009 | 19282630 |
| enhanced crude oil biodegradation and rhamnolipid production by pseudomonas stutzeri strain g11 in the presence of tween-80 and triton x-100. | in this study, the growth of sixty-one bacterial strains in crude oil were determined spectrophotometrically at 620 nm. pseudomonas aeruginosa g1, pseudomonas fluorescens g6, pseudomonas stutzeri g11 and pseudomonas putida g15 were chosen for the study based on the efficiency of crude oil utilisation. at 1% (v/v) crude oil concentration, p. stutzeri g11 strain degraded a maximum of 69%. the percentage of degradation by the p. stutzeri g11 strain decreased from 69% to 59% as the concentration of ... | 2008 | 19297982 |
| isolation of pseudomonas stutzeri in wastewater of catfish fish-ponds in the mekong delta and its application for wastewater treatment. | the aim of this study was to explore the potential for reducing soluble n load in fishpond wastewater using naturally occurring denitrifying bacteria. twenty-seven isolates were selected from in wastewater (liquid/solid) of catfish-ponds located along the tien river, in the mekong delta, vietnam in sw-lb medium (artificial seawater luria-britani medium) supplemented with 10 mm nh4 and no3 and twenty-five isolates were identified as pseudomonas stutzeri based on similarity of pcr-16s rrna using u ... | 2009 | 19299124 |
| in vitro potency of doripenem tested against an international collection of rarely isolated bacterial pathogens. | doripenem, a new 1beta-methyl parenteral carbapenem, has very broad-spectrum activity against gram-positive and gram-negative aerobic bacteria. as noted here, the spectrum and potency extended to many rarely isolated species sampled by the doripenem global surveillance program. among the species or species groups with <or=0.14% prevalence (1959 strains tested), doripenem was active against 98.9% of enterobacteriaceae at <or=0.5 microg/ml. similarly, more than 90% of other rarely isolated gram-ne ... | 2009 | 19302927 |
| accelerator analysis of tributyltin adsorbed onto the surface of a tributyltin resistant marine pseudoalteromonas sp. cell. | tributyltin (tbt) released into seawater from ship hulls is a stable marine pollutant and obviously remains in marine environments. we isolated a tbt resistant marine pseudoalteromonas sp. tbt1 from sediment of a ship's ballast water. the isolate (10(9.3 +/- 0.2) colony-forming units ml(-1)) adsorbed tbt in proportion to the concentrations of tbtcl externally added up to 3 mm, where the number of tbt adsorbed by a single cell was estimated to be 10(8.2). the value was reduced to about one-fifth ... | 2008 | 19325731 |
| [community-acquired pseudomonas stutzeri meningitis in an immunocompetent patient]. | pseudomonas stutzeri which is an aerobic, non-fermentative gram-negative bacillus frequently found in soil, water and hospital environment, rarely leads to serious community-acquired infections. in this report a case of community-acquired meningitis due to p. stutzeri was presented. a 73-years-old male patient was admitted to the emergency department with the complaints of nausea, vomiting, headache, dizziness, difficulties in walking and speaking and loss of consciousness. there was no history ... | 2009 | 19334394 |
| characterization of the drat/drag system for posttranslational regulation of nitrogenase in the endophytic betaproteobacterium azoarcus sp. strain bh72. | drat/drag-mediated posttranslational regulation of the nitrogenase fe protein by adp-ribosylation has been described for a few diazotrophic bacteria belonging to the class alphaproteobacteria. here we present for the first time the drat/drag system of a betaproteobacterium, azoarcus sp. strain bh72, a diazotrophic grass endophyte. its genome harbors one drat ortholog and two physically unlinked genes coding for adp-ribosylhydrolases. northern blot analysis revealed cotranscription of drat with t ... | 2009 | 19346301 |
| intramolecular electron transfer in pseudomonas aeruginosa cd(1) nitrite reductase: thermodynamics and kinetics. | the cd(1) nitrite reductases, which catalyze the reduction of nitrite to nitric oxide, are homodimers of 60 kda subunits, each containing one heme-c and one heme-d(1). heme-c is the electron entry site, whereas heme-d(1) constitutes the catalytic center. the 3d structure of pseudomonas aeruginosa nitrite reductase has been determined in both fully oxidized and reduced states. intramolecular electron transfer (et), between c and d(1) hemes is an essential step in the catalytic cycle. in earlier s ... | 2009 | 19348767 |
| isolation and characterization of pseudomonas stutzeri qz1 from an anoxic sulfide-oxidizing bioreactor. | bacterial strain qz1 was isolated from sludge of anoxic sulfide-oxidizing (aso) reactor. based on 16s rdna sequence analysis and morphological characteristics, the isolate was identified as pseudomonas stutzeri. the isolate was found to be a facultative chemolithotroph, using sulfide as electron donor and nitrite as electron acceptor. the strain qz1 produced sulfate as the major product of sulfide oxidation, depending on the initial sulfide and nitrite concentrations. the isolate was capable of ... | 2009 | 19351560 |
| physiological roles of two dissimilatory nitrate reductases in the deep-sea denitrifier pseudomonas sp. strain mt-1. | the deep-sea denitrifier pseudomonas sp. strain mt-1 has two distinct gene clusters encoding dissimilatory nitrate reductases, periplasmic nitrate reductase (nap) and membrane-bound nitrate reductase (nar). in order to investigate the physiological roles of these enzymes, we determined the nitrate reductase activity of the soluble and membrane fractions from mt-1 and the type strain of pseudomonas stutzeri (closely related with mt-1) grown under various conditions. in mt-1, the activities of bot ... | 2009 | 19352028 |
| growth of pseudomonas chloritidismutans aw-1(t) on n-alkanes with chlorate as electron acceptor. | microbial (per)chlorate reduction is a unique process in which molecular oxygen is formed during the dismutation of chlorite. the oxygen thus formed may be used to degrade hydrocarbons by means of oxygenases under seemingly anoxic conditions. up to now, no bacterium has been described that grows on aliphatic hydrocarbons with chlorate. here, we report that pseudomonas chloritidismutans aw-1(t) grows on n-alkanes (ranging from c7 until c12) with chlorate as electron acceptor. strain aw-1(t) also ... | 2009 | 19352644 |
| structure of phnp, a phosphodiesterase of the carbon-phosphorus lyase pathway for phosphonate degradation. | carbon-phosphorus lyase is a multienzyme system encoded by the phn operon that enables bacteria to metabolize organophosphonates when the preferred nutrient, inorganic phosphate, is scarce. one of the enzymes encoded by this operon, phnp, is predicted by sequence homology to be a metal-dependent hydrolase of the beta-lactamase superfamily. screening with a wide array of hydrolytically sensitive substrates indicated that phnp is an enzyme with phosphodiesterase activity, having the greatest speci ... | 2009 | 19366688 |
| bacterial communities from shoreline environments (costa da morte, northwestern spain) affected by the prestige oil spill. | the bacterial communities in two different shoreline matrices, rocks and sand, from the costa da morte, northwestern spain, were investigated 12 months after being affected by the prestige oil spill. culture-based and culture-independent approaches were used to compare the bacterial diversity present in these environments with that at a nonoiled site. a long-term effect of fuel on the microbial communities in the oiled sand and rock was suggested by the higher proportion of alkane and polyaromat ... | 2009 | 19376924 |
| resistance to peroxynitrite in neisseria gonorrhoeae. | neisseria gonorrhoeae encodes a number of important genes that aid in survival during times of oxidative stress. the same immune cells capable of oxygen-dependent killing mechanisms also have the capacity to generate reactive nitrogen species (rns) that may function antimicrobially. f62 and eight additional gonococcal strains displayed a high level of resistance to peroxynitrite, while neisseria meningitidis and escherichia coli showed a four- to seven-log and a four-log decrease in viability, r ... | 2009 | 19406894 |
| linear alkylbenzene sulfonate tolerance in bacteria isolated from sediment of tropical water bodies polluted with detergents. | the discharge of untreated detergent-bearing waste introduces linear alklcylbenzene sulfonates (las) to the aquatic environment. the surfactant persists in some streams and rivers in nigeria, some is adsorbed to suspended materials and end in the sediment of the receiving water bodies. in this study, bacteria isolated from sediments of some tropical detergent-effluent-polluted streams were tested for tolerance to las using the media dilution technique. las-tolerance was indicated by growth of th ... | 2008 | 19419067 |
| genome sequence of azotobacter vinelandii, an obligate aerobe specialized to support diverse anaerobic metabolic processes. | azotobacter vinelandii is a soil bacterium related to the pseudomonas genus that fixes nitrogen under aerobic conditions while simultaneously protecting nitrogenase from oxygen damage. in response to carbon availability, this organism undergoes a simple differentiation process to form cysts that are resistant to drought and other physical and chemical agents. here we report the complete genome sequence of a. vinelandii dj, which has a single circular genome of 5,365,318 bp. in order to reconcile ... | 2009 | 19429624 |
| genomic and genetic analyses of diversity and plant interactions of pseudomonas fluorescens. | pseudomonas fluorescens are common soil bacteria that can improve plant health through nutrient cycling, pathogen antagonism and induction of plant defenses. the genome sequences of strains sbw25 and pf0-1 were determined and compared to each other and with p. fluorescens pf-5. a functional genomic in vivo expression technology (ivet) screen provided insight into genes used by p. fluorescens in its natural environment and an improved understanding of the ecological significance of diversity with ... | 2009 | 19432983 |
| cultivation characteristics of denitrification by thermophilic geobacillus sp. strain tdn01. | nine thermophilic denitrification bacteria were isolated from field soil, mud, and spa samples. the alignment of 16s rdna showed that all were identical to the genus geobacillus. two of the bacteria produced n2o and n2 gas and the other seven strains produced n2 gas from nitrate. we examined the growth substrates for geobacillus tdn01 and determined that sodium succinate, pyruvate, formate, acetate, glycerol, glucose, sucrose, and cellobiose well supported growth of the isolate. growth occurred ... | 2009 | 19436124 |
| insights into bacterial cellulose biosynthesis by functional metagenomics on antarctic soil samples. | in this study, the mining of an antarctic soil sample by functional metagenomics allowed the isolation of a cold-adapted protein (rbcel1) that hydrolyzes only carboxymethyl cellulose. the new enzyme is related to family 5 of the glycosyl hydrolase (gh5) protein from pseudomonas stutzeri (pst_2494) and does not possess a carbohydrate-binding domain. the protein was produced and purified to homogeneity. rbcel1 displayed an endoglucanase activity, producing cellobiose and cellotriose, using carboxy ... | 2009 | 19458657 |
| broad substrate specificity and catalytic mechanism of pseudomonas stutzeri l-rhamnose isomerase: insights from qm/mm molecular dynamics simulations. | l-rhamnose isomerase (l-rhi) has been found in many microorganisms and catalyzes the reversible isomerization between l-rhamnose and l-rhamnulose. interestingly, pseudomonas stutzeri l-rhi (p. stutzeri l-rhi) exhibits a much broader substrate specificity than others such as escherichia coli l-rhi (e. coli l-rhi) and catalyzes the interconversion of many aldoses and ketoses. to elucidate the uniqueness of p. stutzeri l-rhi and the mechanism of enzymatic catalysis, we performed dual-level combined ... | 2009 | 19472978 |
| the genome sequence of geobacter metallireducens: features of metabolism, physiology and regulation common and dissimilar to geobacter sulfurreducens. | the genome sequence of geobacter metallireducens is the second to be completed from the metal-respiring genus geobacter, and is compared in this report to that of geobacter sulfurreducens in order to understand their metabolic, physiological and regulatory similarities and differences. | 2009 | 19473543 |
| n-vanillylnonanamide tested as a non-toxic antifoulant, applied to surfaces in a polyurethane coating. | the potential on n-vanillylnonanamide (nvn) in preventing the attachment of pseudomonas stutzeri and a bacillus cereus-group strain was investigated. nvn up to 852 microm was not toxic, nor was it an energy source for either organism. microbial attachment assays were carried out on glass and polylysine slides. with nvn being dispersed in or applied to the surfaces using a polyurethane coating. nvn at 205 microm inhibited bacillus adhesion on glass slides by 48% and the percentage did not signifi ... | 2009 | 19488677 |
| assessing bacterial adhesion using dlvo and xdlvo theories and the jet impingement technique. | in this study, the adhesion of two bacterial strains (pseudomonas stutzeri ps, and staphylococcus epidermidis, se) to the glass and the indium tin oxide (ito)-coated glass surfaces was examined qualitatively and quantitatively using the theoretical approaches and the jet impingement technique. a comparison between the dlvo and the extended dlvo (xdlvo) theories showed that the xdlvo predictions of bacterial adhesion and its reversibility are more accurate than dlvo predictions. the adhesion test ... | 2009 | 19493661 |
| phylogenetic characterization of the heterotrophic bacterial communities inhabiting a marine recirculating aquaculture system. | the aim of the present work was to characterize the heterotrophic bacterial community of a marine recirculating aquaculture system (ras). | 2009 | 19508294 |
| tracing the evolution of competence in haemophilus influenzae. | natural competence is the genetically encoded ability of some bacteria to take up dna from the environment. although most of the incoming dna is degraded, occasionally intact homologous fragments can recombine with the chromosome, displacing one resident strand. this potential to use dna as a source of both nutrients and genetic novelty has important implications for the ecology and evolution of competent bacteria. however, it is not known how frequently competence changes during evolution, or w ... | 2009 | 19516897 |
| molecular cloning and expression of a novel trehalose synthase gene from enterobacter hormaechei. | abstract: | 2009 | 19523196 |
| effect of nitrate and acetylene on nirs, cnorb, and nosz expression and denitrification activity in pseudomonas mandelii. | nitrate acts as an electron acceptor in the denitrification process. the effect of nitrate in the range of 0 to 1,000 mg/liter on pseudomonas mandelii nirs, cnorb, and nosz gene expression was studied, using quantitative reverse transcription-quantitative pcr. denitrification activity was measured by using the acetylene blockage method and gas chromatography. the effect of acetylene on gene expression was assessed by comparing denitrification gene expression in p. mandelii culture grown in the p ... | 2009 | 19525277 |
| transcriptional profiling identifies the metabolic phenotype of gonococcal biofilms. | neisseria gonorrhoeae, the etiologic agent of gonorrhea, is frequently asymptomatic in women, often leading to chronic infections. one factor contributing to this may be biofilm formation. n. gonorrhoeae can form biofilms on glass and plastic surfaces. there is also evidence that biofilm formation may occur during natural cervical infection. to further study the mechanism of gonococcal biofilm formation, we compared transcriptional profiles of n. gonorrhoeae biofilms to planktonic profiles. biof ... | 2009 | 19528210 |
| novel denitrifying bacterium ochrobactrum anthropi yd50.2 tolerates high levels of reactive nitrogen oxides. | most studies of bacterial denitrification have used nitrate (no3-) as the first electron acceptor, whereas relatively less is understood about nitrite (no2-) denitrification. we isolated novel bacteria that proliferated in the presence of high levels of no2- (72 mm). strain yd50.2, among several isolates, was taxonomically positioned within the alpha subclass of proteobacteria and identified as ochrobactrum anthropi yd50.2. this strain denitrified no2-, as well as no3-. the gene clusters for den ... | 2009 | 19542343 |
| arsenic detoxification potential of aox genes in arsenite-oxidizing bacteria isolated from natural and constructed wetlands in the republic of korea. | arsenic is subject to microbial interactions, which support a wide range of biogeochemical transformations of elements in natural environments such as wetlands. the arsenic detoxification potential of the bacterial strains was investigated with the arsenite oxidation gene, aox genotype, which were isolated from the natural and constructed wetlands. the isolates were able to grow in the presence of 10 mm of sodium arsenite (as(iii) as naaso(2)) and 1 mm of d: +glucose. phylogenetic analysis based ... | 2010 | 19548094 |
| antibiotic susceptibility profiles of uncommon bacterial species causing severe infections in italy. | this study presents the results of the italian "severe infections project" involving bacteria that can be considered rare causes of disease. we isolated 30 uncommon human pathogens from a total of 60 strains (1.2% of all the isolates). the most frequent sources of uncommon human pathogens were primary bloodstream infections (48.3%) and pneumonia (20%). species such as comamonas testosteroni, enterococcus hirae, kluyvera ascorbata, kluyvera cryocrescens, leclercia adecarboxylata and ochrobactrum ... | 2009 | 19567344 |
| the complete genome of teredinibacter turnerae t7901: an intracellular endosymbiont of marine wood-boring bivalves (shipworms). | here we report the complete genome sequence of teredinibacter turnerae t7901. t. turnerae is a marine gamma proteobacterium that occurs as an intracellular endosymbiont in the gills of wood-boring marine bivalves of the family teredinidae (shipworms). this species is the sole cultivated member of an endosymbiotic consortium thought to provide the host with enzymes, including cellulases and nitrogenase, critical for digestion of wood and supplementation of the host's nitrogen-deficient diet. t. t ... | 2009 | 19568419 |
| arsenic accumulation by pseudomonas stutzeri and its response to some thiol chelators. | the aim of this study is to examine arsenic accumulation by pseudomonas stutzeri and its response to some thiol chelators, dmps and miadmsa. | 2008 | 19568912 |
| enhancing biodegradation of wastewater by microbial consortia with fractional factorial design. | batch experiments were conducted on the degradation of synthetic and municipal wastewater by six different strains, i.e., agrobacterium sp., bacillus sp., enterobacter cloacae, gordonia, pseudomonas stutzeri, pseudomonas putida. by applying a fractional factorial design (ffd) of experiments, the influence of each strain and their interactions were quantified. an empirical model predicting the treatment efficiency was built based on the results of the ffd experiments with an r(2) value of 99.39%. ... | 2009 | 19632773 |
| persistent wolbachia and cultivable bacteria infection in the reproductive and somatic tissues of the mosquito vector aedes albopictus. | commensal and symbiotic microbes have a considerable impact on the behavior of many arthropod hosts, including hematophagous species that transmit pathogens causing infectious diseases to human and animals. little is known about the bacteria associated with mosquitoes other than the vectorized pathogens. this study investigated wolbachia and cultivable bacteria that persist through generations in ae. albopictus organs known to host transmitted arboviruses, such as dengue and chikungunya. | 2009 | 19633721 |
| [analysis of a new nitrogen fixation gene in pseudomonas stutzeri a1501]. | we studied the role of the nitrogen fixation gene pst1305 located within the nitrogen fixation island of pseudomonas stutzeri a1501. | 2009 | 19637563 |
| ntrc-dependent regulatory network for nitrogen assimilation in pseudomonas putida. | pseudomonas putida kt2440 is a model strain for studying bacterial biodegradation processes. however, very little is known about nitrogen regulation in this strain. here, we show that the nitrogen regulatory ntrc proteins from p. putida and escherichia coli are functionally equivalent and that substitutions leading to partially active forms of enterobacterial ntrc provoke the same phenotypes in p. putida ntrc. p. putida has only a single p(ii)-like protein, encoded by glnk, whose expression is n ... | 2009 | 19648236 |
| efflux-mediated drug resistance in bacteria: an update. | drug efflux pumps play a key role in drug resistance and also serve other functions in bacteria. there has been a growing list of multidrug and drug-specific efflux pumps characterized from bacteria of human, animal, plant and environmental origins. these pumps are mostly encoded on the chromosome, although they can also be plasmid-encoded. a previous article in this journal provided a comprehensive review regarding efflux-mediated drug resistance in bacteria. in the past 5 years, significant pr ... | 2009 | 19678712 |
| outcome of acute prosthetic joint infections due to gram-negative bacilli treated with open debridement and retention of the prosthesis. | the aim of our study was to evaluate the outcome of acute prosthetic joint infections (pjis) due to gram-negative bacilli (gnb) treated without implant removal. patients with an acute pji due to gnb diagnosed from 2000 to 2007 were prospectively registered. demographics, comorbidity, type of implant, microbiology data, surgical treatment, antimicrobial therapy, and outcome were recorded. classification and regression tree analysis, the kaplan-meier survival method, and the cox regression model w ... | 2009 | 19687237 |
| biphenyl-metabolizing bacteria in the rhizosphere of horseradish and bulk soil contaminated by polychlorinated biphenyls as revealed by stable isotope probing. | dna-based stable isotope probing in combination with terminal restriction fragment length polymorphism was used in order to identify members of the microbial community that metabolize biphenyl in the rhizosphere of horseradish (armoracia rusticana) cultivated in soil contaminated with polychlorinated biphenyls (pcbs) compared to members of the microbial community in initial, uncultivated bulk soil. on the basis of early and recurrent detection of their 16s rrna genes in clone libraries construct ... | 2009 | 19700551 |
| a novel insertion sequence derepresses efflux pump expression and preadapts pseudomonas putida s12 for extreme solvent stress. | a multidrug efflux pump, srpabc, plays a key role in pseudomonas putida s12 tolerance to toxic organic solvents. srprs are putative regulators of the srpabc efflux pump encoded upstream of the srpabc structural genes, and previous studies suggest that srps is a repressor of srpabc expression. an s12 isolate able to withstand extreme solvent stress carries a novel insertion sequence, isppu21, interrupting srps. this insertion preadapts s12 to extreme solvent conditions through constitutive srpabc ... | 2009 | 19717594 |
| single-residue changes in the c-terminal disulfide-bonded loop of the pseudomonas aeruginosa type iv pilin influence pilus assembly and twitching motility. | pila, the major pilin subunit of pseudomonas aeruginosa type iv pili (t4p), is a principal structural component. pila has a conserved c-terminal disulfide-bonded loop (dsl) that has been implicated as the pilus adhesinotope. structural studies have suggested that dsl is involved in intersubunit interactions within the pilus fiber. pila mutants with single-residue substitutions, insertions, or deletions in the dsl were tested for pilin stability, pilus assembly, and t4p function. mutation of eith ... | 2009 | 19717595 |
| nitrite reductase genes in halobenzoate degrading denitrifying bacteria. | abstract diversity of the functional genes encoding dissimilatory nitrite reductase was investigated for the first time in denitrifying halobenzoate degrading bacteria and in two 4-chlorobenzoate degrading denitrifying consortia. nitrite reductase genes were pcr-amplified with degenerate primers (specific to the two different types of respiratory nitrite reductase, nirs and nirk), cloned and sequenced to determine which type of nitrite reductase was present in each isolate and consortium. halobe ... | 2003 | 19719666 |
| expression of nifh genes by diazotrophic bacteria in the rhizosphere of short form spartina alterniflora. | abstract a diverse assemblage of diazotrophic bacteria exists in the rhizosphere of the smooth cordgrass, spartina alterniflora, but the taxa actively involved in nitrogen fixation have not been determined. in order to identify the diazotrophs that were actively expressing nifh, the gene encoding the nitrogenase iron protein, mrna was extracted from spartina rhizosphere samples and nifh-specific seminested reverse transcriptase-pcr performed. expressed nifh sequences were recovered from organism ... | 2003 | 19719672 |
| transverse bacterial migration induced by chemotaxis in a packed column with structured physical heterogeneity. | the significance of chemotaxis in directing bacterial migration toward contaminants in natural porous media was investigated under groundwater flow conditions. a laboratory-scale column, with a coarse-grained sand core surrounded by a fine-grained annulus, was used to simulate natural aquifers with strata of different hydraulic conductivities. a chemoattractant source was placed along the central axis of the column to model contaminants trapped in the heterogeneous subsurface. chemotactic bacter ... | 2009 | 19731698 |
| ecophysiology and the energetic benefit of mixotrophic fe(ii) oxidation by various strains of nitrate-reducing bacteria. | in order to assess the importance of nitrate-dependent fe(ii) oxidation and its impact on the growth physiology of dominant fe oxidizers, we counted these bacteria in freshwater lake sediments and studied their growth physiology. most probable number counts of nitrate-reducing fe(ii)-oxidizing bacteria in the sediment of lake constance, a freshwater lake in southern germany, yielded about 10(5) cells ml(-1) of the total heterotrophic nitrate-reducing bacteria, with about 1% (10(3) cells ml(-1)) ... | 2009 | 19732145 |
| the complete genome of comamonas testosteroni reveals its genetic adaptations to changing environments. | members of the gram-negative, strictly aerobic genus comamonas occur in various environments. here we report the complete genome of comamonas testosteroni strain cnb-2. strain cnb-2 has a circular chromosome that is 5,373,643 bp long and has a g+c content of 61.4%. a total of 4,803 open reading frames (orfs) were identified; 3,514 of these orfs are functionally assigned to energy production, cell growth, signal transduction, or transportation, while 866 orfs encode hypothetical proteins and 423 ... | 2009 | 19734336 |
| rapid isolation of novel fk506 binding proteins from multiple organisms using gdna and cdna t7 phage display. | reverse chemical proteomics using t7 phage display is a powerful technique for identifying cellular receptors of biologically active small molecules. however, to date this method has generally been limited to cdna libraries constructed from mrna isolated from eukaryotes. in this paper, we describe the construction of the first prokaryotic t7 phage display libraries from randomly digested pseudomonas stutzeri and vibrio fischeri gdna, as well as a plant cdna library from arabidopsis thaliana. we ... | 2009 | 19740670 |
| biochemical and genomic analysis of the denitrification pathway within the genus neisseria. | since neisseria gonorrhoeae and neisseria meningitidis are obligate human pathogens, a comparison with commensal species of the same genus could reveal differences important in pathogenesis. the recent completion of commensal neisseria genome draft assemblies allowed us to perform a comparison of the genes involved in the catalysis, assembly and regulation of the denitrification pathway, which has been implicated in the virulence of several bacteria. all species contained a highly conserved nitr ... | 2009 | 19762442 |
| molybdenum trafficking for nitrogen fixation. | the molybdenum nitrogenase is responsible for most biological nitrogen fixation, a prokaryotic metabolic process that determines the global biogeochemical cycles of nitrogen and carbon. here we describe the trafficking of molybdenum for nitrogen fixation in the model diazotrophic bacterium azotobacter vinelandii. the genes and proteins involved in molybdenum uptake, homeostasis, storage, regulation, and nitrogenase cofactor biosynthesis are reviewed. molybdenum biochemistry in a. vinelandii reve ... | 2009 | 19772354 |
| physiological role of nahw, the additional salicylate hydroxylase found in pseudomonas stutzeri an10. | the physiological role of nahw, the second salicylate hydroxylase of pseudomonas stutzeri an10, has been analysed by gene mutation and further complementation. when grown on naphthalene as a unique carbon and energy source, the nahw mutant showed a strong decrease in salicylate hydroxylase activity when compared with the wild-type strain, exhibited lower specific growth rates and accumulated salicylate in culture supernatants. similarly, lower specific growth rates and salicylate accumulation we ... | 2009 | 19796135 |
| a novel rpmxr motif among class ii 5-enolpyruvylshikimate-3-phosphate synthases is required for enzymatic activity and glyphosate resistance. | the shikimate pathway enzyme 5-enolpyruvylshikimate-3-phosphate (epsp) synthase is an attractive target for drugs and herbicides. here we identified a novel rpmxr motif that is strictly conserved among class ii epsp synthases. site-directed mutational analysis of this motif showed that substitutions of the four strictly conserved amino acid residues, arg127, pro128, met129, and arg131, resulted in complete loss of enzymatic activity, whereas changes in the non-conserved asn130 residue strongly i ... | 2009 | 19799945 |
| nitric oxide signaling in pseudomonas aeruginosa biofilms mediates phosphodiesterase activity, decreased cyclic di-gmp levels, and enhanced dispersal. | bacteria in biofilms often undergo active dispersal events and revert to a free-swimming, planktonic state to complete the biofilm life cycle. the signaling molecule nitric oxide (no) was previously found to trigger biofilm dispersal in the opportunistic pathogen pseudomonas aeruginosa at low, nontoxic concentrations (n. barraud, d. j. hassett, s. h. hwang, s. a. rice, s. kjelleberg, and j. s. webb, j. bacteriol. 188:7344-7353, 2006). no was further shown to increase cell motility and susceptibi ... | 2009 | 19801410 |
| glutaredoxins: roles in iron homeostasis. | glutaredoxins, proteins traditionally involved in redox reactions, are also required for iron-sulfur cluster assembly and haem biosynthesis. these new roles are probably related to the ability of some glutaredoxins to bind labile [2fe-2s] clusters and to transfer them rapidly and efficiently to acceptor proteins. recent results point to putative roles for glutaredoxins in the sensing of cellular iron and in iron-sulfur cluster biogenesis, either as scaffold proteins for the de novo synthesis of ... | 2010 | 19811920 |
| glutaredoxins: roles in iron homeostasis. | glutaredoxins, proteins traditionally involved in redox reactions, are also required for iron-sulfur cluster assembly and haem biosynthesis. these new roles are probably related to the ability of some glutaredoxins to bind labile [2fe-2s] clusters and to transfer them rapidly and efficiently to acceptor proteins. recent results point to putative roles for glutaredoxins in the sensing of cellular iron and in iron-sulfur cluster biogenesis, either as scaffold proteins for the de novo synthesis of ... | 2010 | 19811920 |