| characterization and phylogenetic analysis of biodemulsifier-producing bacteria. | based on demulsification performance, twenty biodemulsifier-producing strains were isolated from various environmental sources. five of them achieved nearly or over 90% of emulsion breaking ratio within 24 h. with the aid of biochemical and physiological tests and 16s rdna analysis, these isolates were classified into eleven genera, in which six genera (brevibacillus sp., dietzia sp., ochrobactrum sp., pusillimonas sp., sphingopyxis sp. and achromobacter sp.) were firstly reported as demulsifyin ... | 2010 | 19720525 |
| real-time electrochemical monitoring of the polymerase chain reaction by mediated redox catalysis. | we described the proof-of-principle of a nonoptical real-time pcr that uses cyclic voltammetry for indirectly monitoring the amplified dna product generated in the pcr reaction solution after each pcr cycle. to enable indirect measurement of the amplicon produced throughout pcr, we monitor electrochemically the progressive consumption (i.e., the decrease of concentration) of free electroactive deoxynucleoside triphosphates (dntps) used for dna synthesis. this is accomplished by exploiting the fa ... | 2009 | 19722651 |
| clinical, microbiological profile and treatment outcome of ocular infections caused by achromobacter xylosoxidans. | to study the microbiological profile and outcome of ocular infections caused by achromobacter xylosoxidans. | 2009 | 19730091 |
| eiseniicola composti gen. nov., sp. nov., with antifungal activity against plant pathogenic fungi. | a gram-negative, short rod-shaped bacterial strain, yc06271t, was isolated from the vermicompost (vc) collected at masan, korea and its taxonomic position was investigated by a polyphasic taxonomic approach. strain yc06271t grew optimally at 28-30 degrees c and at ph 7.0-9.0. the 16s rrna gene sequence of strain yc06271t was most closely related to members of the genera bordetella (96.4-95.8 %), achromobacter (96.0-95.7 %), alcaligenes (96.0-94.2 %), pusillimonas noertemannii (95.9 %), pigmentip ... | 2010 | 19734285 |
| eurocarecf quality assessment of diagnostic microbiology of cystic fibrosis isolates. | the identification of microbial species from respiratory specimens and their susceptibility to antimicrobial agents are among the most important diagnostic measures of care for patients with cystic fibrosis (cf). under the umbrella of eurocarecf, two quality assurance trials of cf microbiology were performed in 2007 and 2008. nine formulations with cf bacterial isolates were dispatched. a total of 31/37 laboratories from 18/21 european countries participated in the 2007 and 2008 trials. the comm ... | 2009 | 19741077 |
| pusillimonas ginsengisoli sp. nov., isolated from soil of a ginseng field. | two novel strains of gram-negative, non-sporulating, short rod-shaped, motile bacteria, designated dcy25t and dcy28, were isolated from soil of a ginseng field in south korea and characterized in order to determine their taxonomic position. 16s rrna gene sequence analysis showed that strains dcy25t and dcy28 belonged to the betaproteobacteria, the highest sequence similarities being found with pusillimonas noertemannii bn9t (96.9%), bordetella trematum dsm 11334(t) (95.9%), achromobacter denitri ... | 2010 | 19749028 |
| heterologous expression of the methyl carbamate-degrading hydrolase mcd. | the methyl carbamate-degrading hydrolase (mcd) of achromobacter wm111 has considerable potential as a pesticide bioremediation agent. however this potential has been unrealisable until now because of an inability to express mcd in heterologous hosts such as escherichia coli. herein, we describe the first successful attempt to express appreciable quantities of mcd in active form in e. coli, and the subsequent characterisation of the heterologously expressed material. we find that the properties o ... | 2009 | 19770008 |
| [psoas and paravesical abscess formation due to achromobacter xylosoxidans: a case report]. | | 2010 | 19775779 |
| microbial utilization of the industrial wastewater pollutants 2-ethylhexylthioglycolic acid and iso-octylthioglycolic acid by aerobic gram-negative bacteria. | industrial wastewater from the production of sulfur containing esters and the resulting products of this synthesis, 2-ethylhexylthioglycolic acid (ehtg) and iso-octylthioglycolic acid (iotg), were deployed in this study to enrich novel bacterial strains, since no wastewater and ehtg or iotg degrading microorganisms were hitherto described or available. in addition, nothing is known about the biodegradation of these thiochemicals. the effect of this specific wastewater on the growth behaviour of ... | 2010 | 19789984 |
| bacterial diversity in water injection systems of brazilian offshore oil platforms. | biogenic souring and microbial-influenced corrosion is a common scenario in water-flooded petroleum reservoirs. water injection systems are continuously treated to control bacterial contamination, but some bacteria that cause souring and corrosion can persist even after different treatments have been applied. our aim was to increase our knowledge of the bacterial communities that persist in the water injection systems of three offshore oil platforms in brazil. to achieve this goal, we used a cul ... | 2010 | 19830416 |
| a case of endocarditis of difficult diagnosis in dialysis: could "pest" friends be involved? | we report a case of a. xylosoxidans endocarditis of larvate clinical presentation in a central venous catheter-dependent hemodialysis patient of difficult diagnosis and poor evolution despite a high index of suspicion and consequent assessment. a 50-year-old man on hemodialysis presented with inflammatory-malnutrition parameters during the months prior to diagnosis of endocarditis, whilst he was otherwise asymptomatic. no vegetations were detectable on his cardiac valves at repeated echocardiogr ... | 2009 | 19863886 |
| in vitro susceptibility of achromobacter spp. isolates: comparison of disk diffusion, etest and agar dilution methods. | in this study, we analysed the antimicrobial susceptibility of 92 strains of achromobacter spp. isolated from clinical samples to 18 antimicrobial agents. the disk diffusion method and etest were compared with the agar dilution method, and the breakpoints of susceptibility and resistance for the disk diffusion method for the antimicrobials tested were determined. the most active antibiotics were piperacillin, piperacillin/tazobactam and the carbapenems. by applying the linear least-squares regre ... | 2010 | 19889520 |
| structural basis of inter-protein electron transfer for nitrite reduction in denitrification. | recent earth science studies have pointed out that massive acceleration of the global nitrogen cycle by anthropogenic addition of bio-available nitrogen has led to a host of environmental problems. nitrous oxide (n(2)o) is a greenhouse gas that is an intermediate during the biological process known as denitrification. copper-containing nitrite reductase (cunir) is a key enzyme in the process; it produces a precursor for n(2)o by catalysing the one-electron reduction of nitrite (no2-) to nitric o ... | 2009 | 19890332 |
| estimating the cellular maintenance coefficient and its use in the design of two-phase partitioning bioscrubbers. | one of the key roles of an organic solvent has emerged to be the enhancement of oxygen transfer in two-phase partitioning bioscrubbers (tppbs). in order to determine an optimum organic fraction for a given vocs loading, the oxygen demand of the total cell mass must be estimated, which depends upon the magnitude of the cellular maintenance coefficient. we have estimated the dynamics of the maintenance coefficient for benzene degradation by achromobacter xylosoxidans y234 in a tppb and found that ... | 2010 | 19915871 |
| got black swimming dots in your cell culture? identification of achromobacter as a novel cell culture contaminant. | cell culture model systems are utilized for their ease of use, relative inexpensiveness, and potentially limitless sample size. reliable results cannot be obtained, however, when cultures contain contamination. this report discusses the observation and identification of mobile black specks observed in multiple cell lines. cultures of the contamination were grown, and dna was purified from isolated colonies. the 16s rdna gene was pcr amplified using primers that will amplify the gene from many ge ... | 2010 | 19926304 |
| inflammation in achromobacter xylosoxidans infected cystic fibrosis patients. | achromobacter xylosoxidans infection may cause conspicuous chronic pulmonary inflammation in cystic fibrosis (cf) patients similar to pseudomonas aeruginosa and the burkholderia cepacia complex (bcc). evolution in lung function was compared in chronically infected patients. cytokine concentrations in cf patients with and without chronic infection were compared to healthy controls. | 2010 | 19939747 |
| decolorization of anthraquinone vat blue 4 by the free cells of an autochthonous bacterium, bacillus subtilis. | uncontaminated soil, vat blue 4 contaminated soil and vat blue 4 effluent were screened for heterotrophic bacterial population and the bacterial density were found to be 19.3 x 10(4) colony forming units (cfu)/gm, 5.5 x 10(4) cfu/gm and 1.1 x 10(4) cfu/ml respectively. student's 't' test analysis affirmed that significant variation prevailed between the three set of 't' tests conducted (p<0.001 to 0.002). the heterotrophic bacterial population of dye contaminated soil comprised of 32.5% of pseud ... | 2009 | 19955647 |
| molecular analysis of the bacterial microbiota in duodenal biopsies from dogs with idiopathic inflammatory bowel disease. | an association between mucosa-adherent commensal bacteria and inflammatory bowel disease (ibd) has been proposed for humans. there are no reports characterizing the mucosa-adherent duodenal microbiota in dogs with idiopathic ibd using molecular methods. the aim of this study was to investigate differences in the mucosa-adherent duodenal microbiota between dogs with idiopathic ibd and healthy dogs. duodenal biopsy samples were collected from seven dogs with ibd and seven healthy control dogs. dna ... | 2010 | 19959301 |
| non-covalent interactions in blue copper protein probed by met16 mutation and electronic and resonance raman spectroscopy of achromobacter cycloclastes pseudoazurin. | we have used low-temperature (77k) resonance raman (rr) spectroscopy as a probe of the electronic and molecular structure to investigate weak pi-pi interactions between the metal ion-coordinated his imidazoles and aromatic side chains in the second coordination sphere of blue copper proteins. for this purpose, the rr spectra of met16 mutants of achromobacter cycloclastes pseudoazurin (acpaz) with aromatic (met16tyr, met16trp, and met16phe) and aliphatic (met16ala, met16val, met16leu, and met16il ... | 2010 | 20007000 |
| achromobacter xylosoxidans presenting as a suprapatellar abscess and polyarthritis. | | 2010 | 20051759 |
| [catheter- associated bacteremia caused by ochrobactrum anthropi]. | ochrobactrum anthropi is a non-glucose fermentative, aerobic gram-negative bacillus, formerly known as achromobacter sp or cdc group vd. it has been isolated from the environment and from infections in usually immunocompromised human beings. the documented infections frequently involved catheter related bacteremia whereas endophthalmitis, urinary infections, meningitis, endocarditis, hepatic abscess, osteochondritis, pelvic abscess and pancreatic abscess were rarely involved. here it is presente ... | 2009 | 20053608 |
| [aniline removal by aerobic granules and high-efficiency aniline-degrading bacteria]. | aerobic granules were successfully cultivated with aniline as the sole source of carbon and nitrogen. this system could effectively degrade aniline as high as 6 000 mg/l. two aniline-degrading strains which were named as adx1 and adx3, were isolated and purified from the aerobic granular sludge. the degradation characteristics of these two bacteria were different. the strain adxl possessed a relatively higher degradation rate of aniline, while the strain adx3 could stand up to a higher concentra ... | 2009 | 20063750 |
| achromobacter xylosoxidans genomic characterization and correlation of randomly amplified polymorphic dna profiles with relevant clinical features [corrected] of cystic fibrosis patients. | achromobacter xylosoxidans is an emerging pathogen increasingly being isolated from respiratory samples of cystic fibrosis (cf) patients. its role and clinical significance in lung pathogenesis have not yet been clarified. the aim of the present study was to genetically characterize a. xylosoxidans strains isolated from cf patients by use of randomly amplified polymorphic dna (rapd) profiles and to look for a possible correlation between rapd profiles and the patients' clinical features, such as ... | 2010 | 20107101 |
| isolate-specific effects of patulin, penicillic acid and edta on biofilm formation and growth of dental unit water line biofilm isolates. | dental unit water line (duwl) contamination by opportunistic pathogens has its significance in nosocomial infection of patients, health care workers, and life-threatening infections to immunocompromized persons. recently, the quorum sensing (qs) system of duwl isolates has been found to affect their biofilm-forming ability, making it an attractive target for antimicrobial therapy. in this study, the effect of two quorum-sensing inhibitory compounds (patulin; pat, penicillic acid; pa) and edta on ... | 2010 | 20111864 |
| screening of bacterial isolates from polluted soils exhibiting catalase and peroxidase activity and diversity of their responses to oxidative stress. | for the survival of individual isolates of gram-negative bacteria pseudomonas putida, achromobacter xylosoxidans, and the gram-positive bacterium bacillus megaterium, in an environment polluted with crude oil products, the production of catalases exhibiting both catalase and dianisidine-peroxidase activity is important. electrophoretic resolution of cell-free extracts of aerobically grown strains in luria-bertani medium during exponential phase revealed distinctive expression of catalatic and pe ... | 2010 | 20145932 |
| characterization of phosphobacteria isolated from eutrophic aquatic ecosystems. | phosphobacteria are able to enhance phosphorus availability in soil and improve crop yields. to develop such biofertilizers, 14 predominant phosphobacteria were isolated from eutrophic aquatic ecosystems. molecular identification and phylogenetic analysis revealed three groups among the nine isolates of inorganic phosphate-solubilizing bacteria (ipsb): ipsb1 and ipsb2 belonged to the actinobacteria and flavobacteria, respectively, and the other seven belonged to the gamma-proteobacteria. among f ... | 2009 | 20170019 |
| characterization of cycp gene expression in achromobacter xylosoxidans ncimb 11015 and high-level heterologous synthesis of cytochrome c' in escherichia coli. | the cycp gene encoding a periplasmic cytochrome c' from the denitrifying beta-proteobacterium achromobacter xylosoxidans was characterized. the genes flanking cycp encode components of a mobile genetic element characteristic of the beta-proteobacteria, suggesting that cycp has inserted within a transposon or insertion element. the gene therefore does not form part of a denitrification operon or gene cluster. the level of expression of the cycp gene and the level of synthesis of its corresponding ... | 2010 | 20185933 |
| changes in cystic fibrosis sputum microbiology in the united states between 1995 and 2008. | the study objective was to identify changes in cystic fibrosis (cf) sputum microbiology over 13 years. | 2010 | 20232473 |
| the effect of boric acid on the respiration and luminescence of achromobacter fischeri. | | 1946 | 20282328 |
| arsenic-resistant bacteria associated with roots of the wild cirsium arvense (l.) plant from an arsenic polluted soil, and screening of potential plant growth-promoting characteristics. | a rhizobacterial community, associated with the roots of wild thistle cirsium arvense (l.) growing in an arsenic polluted soil, was studied by fluorescence in situ hybridization (fish) analysis in conjunction with cultivation-based methods. in the bulk, rhizosphere, and rhizoplane fractions of the soil, the qualitative picture obtained by fish analysis of the main phylogenetic bacterial groups was similar and was predominantly comprised of alphaproteobacteria, betaproteobacteria, and gammaproteo ... | 2010 | 20303688 |
| a taxonomic study of the mesophilic achromobacter. | | 1947 | 20344231 |
| effects of plant genotype and growth stage on the betaproteobacterial communities associated with different potato cultivars in two fields. | bacterial communities in the rhizosphere are dynamic and susceptible to changes in plant conditions. among the bacteria, the betaproteobacteria play key roles in nutrient cycling and plant growth promotion, and hence the dynamics of their community structures in the rhizosphere should be investigated. here, the effects of plant cultivar, growth stage, and soil type on the communities associated with potato cultivars aveka, aventra, karnico, modena, premiere, and désirée were assessed for two dif ... | 2010 | 20363788 |
| endophytic bacteria improve seedling growth of sunflower under water stress, produce salicylic acid, and inhibit growth of pathogenic fungi. | endophytic bacterial strains sf2 (99.9% homology with achromobacter xylosoxidans), and sf3 and sf4 (99.9% homology with bacillus pumilus) isolated from sunflower grown under irrigation or drought were selected on the basis of plant growth-promoting bacteria (pgpb) characteristics. aims of the study were to examine effects of inoculation with sf2, sf3, and sf4 on sunflower cultivated under water stress, to evaluate salicylic acid (sa) production by these strains in control medium or at ψa = -2.03 ... | 2010 | 20383767 |
| consortia modulation of the stress response: proteomic analysis of single strain versus mixed culture. | the high complexity of naturally occurring microbial communities is the major drawback limiting the study of these important biological systems. in this study, a comparison between pure cultures of pseudomonas reinekei sp. strain mt1 and stable community cultures composed of mt1 plus the addition of achromobacter xylosoxidans strain mt3 (in a steady-state proportion 9:1) was used as a model system to study bacterial interactions that take place under simultaneous chemical and oxidative stress. b ... | 2010 | 20406297 |
| arsenite oxidase from ralstonia sp. 22: characterization of the enzyme and its interaction with soluble cytochromes. | we characterized the aro arsenite oxidation system in the novel strain ralstonia sp. 22, a beta-proteobacterium isolated from soil samples of the salsigne mine in southern france. the inducible aro system consists of a heterodimeric membrane-associated enzyme reacting with a dedicated soluble cytochrome c(554). our biochemical results suggest that the weak association of the enzyme to the membrane probably arises from a still unknown interaction partner. analysis of the phylogeny of the aro gene ... | 2010 | 20421652 |
| in vitro synergistic activity of combined piperacillin and tobramycin against clinical strains of achromobacter xylosoxidans. | | 2010 | 20435577 |
| isolation of phylogenetically diverse nonylphenol ethoxylate-degrading bacteria and characterization of their corresponding biotransformation pathways. | most nonylphenol ethoxylate (npeo)-degrading isolates have been assigned to gamma-proteobacteria, which is different from the results acquired by using molecular ecological techniques. to better understand the environmental fate of npeos, bacterial isolation strategy characterized by the use of gellan gum as a gelling reagent and a low concentration of target carbon source were used to isolate phylogenetically diverse npeo-degrading bacteria from activated sludge, and the biotransformation pathw ... | 2010 | 20457466 |
| bilateral achromobacter xylosoxidans keratitis after laser in situ keratomileusis. | a 31-year-old man was referred to us 2 months after bilateral laser in situ keratomileusis (lasik). on presentation, the corrected distance visual acuity was hand motion in the right eye and 20/25 in the left eye. slitlamp examination showed a diffuse central stromal infiltrate, flap melting, and hypopyon in the right eye and marked interface opacities with crystal-like edges in the left eye. flap lift and irrigation were performed. because of the progressive keratitis, penetrating keratoplasty ... | 2010 | 20494781 |
| newer antibacterial agents and their potential role in cystic fibrosis pulmonary exacerbation management. | pulmonary exacerbations in cystic fibrosis (cf) are frequent events and account for a substantial proportion of the burden of morbidity and mortality in this disease. antibacterial therapies to treat pulmonary exacerbations are instituted empirically and are individualized based on both patient factors (severity of exacerbation, frequency of exacerbation, recent courses of anti-infectives) and pathogen factors (previously isolated pathogens and in vitro predicted susceptibilities). however, the ... | 2010 | 20605846 |
| backbone cyclic insulin. | backbone cyclic insulin was designed and prepared by reverse proteolysis in partial organic solvent of a single-chain precursor expressed in yeast. the precursor contains two loops to bridge the two chains of native insulin. the cyclisation method uses achromobacter lyticus protease and should be generally applicable to proteins with c-terminal lysine and proximal n-terminal. the presence of the ring-closing bond and the native insulin disulfide patterns were documented by lc-ms peptide maps. th ... | 2010 | 20641002 |
| bioremediation of glyphosate-contaminated soils. | based on the results of laboratory and field experiments, we performed a comprehensive assessment of the bioremediation efficiency of glyphosate-contaminated soddy-podzol soil. the selected bacterial strains achromobacter sp. kg 16 (vkm b-2534d) and ochrobactrum anthropi gpk 3 (vkm b-2554d) were used for the aerobic degradation of glyphosate. they demonstrated high viability in soil with the tenfold higher content of glyphosate than the recommended dose for the single in situ treatment of weeds. ... | 2010 | 20676632 |
| a strategic approach for the design and operation of two-phase partitioning bioscrubbers for the treatment of volatile organic compounds. | a strategic approach for the design of two-phase partitioning bioscrubbers (tppbs) has been formulated using, as a basis, a re-evaluation of extensive literature data available for the degradation of benzene by achromobacter xylosoxidans y234 in tppbs with n-hexadecane as the partitioning phase. using a previously determined maintenance coefficient for benzene, we determined that an inlet benzene loading rate of 100 mg/h requires 5,928 mg cell mass at biological steady state and 243.0 mg o(2) /h ... | 2010 | 20718037 |
| long-term hg pollution-induced structural shifts of bacterial community in the terrestrial isopod (porcellio scaber) gut. | in previous studies we detected lower species richness and lower hg sensitivity of the bacteria present in egested guts of porcellio scaber (crustacea, isopoda) from chronically hg polluted than from unpolluted environment. basis for such results were further investigated by sequencing of 16s rrna genes of mercury-resistant (hgr) isolates and clone libraries. we observed up to 385 times higher numbers of hgr bacteria in guts of animals from polluted than from unpolluted environment. the majority ... | 2010 | 20724045 |
| [achromobacter xylosoxidans colonisation in bronchiectasis]. | | 2010 | 20846757 |
| microbial community differences between propionate-fed microbial fuel cell systems under open and closed circuit conditions. | we report the electrochemical characterization and microbial community analysis of closed circuit microbial fuel cells (cc-mfcs) and open circuit (oc) cells continuously fed with propionate as substrate. differences in power output between mfcs correlated with their polarization behavior, which is related to the maturation of the anodophilic communities. the microbial communities residing in the biofilm growing on the electrode, biofouled cation-exchange membrane and anodic chamber liquor of oc- ... | 2010 | 20922377 |
| selection and characteristics of a switchgrass-colonizing microbial community to produce extracellular cellulases and xylanases. | a microbial community was selected for growth on dried and naoh-treated switchgrass. during a 14-day liquid cultivation, a 70% loss in dry weight was observed during the first 4 days and after 14 days, the hemicellulose and cellulose in the system were degraded by 73.5% and 67.3%, respectively. the carboxymethyl cellulase (cmcase) and xylanase levels reached 0.21 and 3.75 iu, respectively. the optimal ph for cmcase and xylanase activities was 5 and 6, respectively. the optimal reaction temperatu ... | 2010 | 20933405 |
| achromobacter marplatensis sp. nov., isolated from a pentachlorophenol contaminated soil. | a polyphasic taxonomic approach was applied for the study of a gram negative bacterium (b2(t)) isolated from soil by selective enrichment with pentachlorophenol. the 16s rrna gene sequence analysis of strain b2(t) showed that this strain belongs to the genus achromobacter, within the betaproteobacteria. the 16s rrna gene sequence possessing more than 99% similarity to the sequences of the type strains of all species in achromobacter, with the highest sequence similarities to those of a. spanius ... | 2010 | 20952547 |
| new vector tools with a hygromycin resistance marker for use with opportunistic pathogens. | the ability of many bacterial strains to tolerate antibiotics can limit the number of molecular tools available for research of these organisms. to help address this problem, we have modified a diverse set of vectors to include a broadly expressed hygromycin resistance (hmr) marker. hygromycin b is an aminoglycoside antibiotic not used to treat infections in humans and has antimicrobial activity against a wide range of microorganisms. vectors with four replication origins are represented, with p ... | 2011 | 20972648 |
| occurrence of gram-negative bacteria in hens' eggs depending on their source and storage conditions. | the aim of this study was to analyse the qualitative composition of gram-negative microbes, mainly of the family enterobacteriaceae, including pathogenic bacteria such as salmonella, in the albumens and yolks and on the shells of hens' eggs, depending on their source and on the temperature and duration of their storage. a total of 375 table eggs were studied, from a large-scale poultry farm, a small-scale poultry farm and a supermarket. each group was divided into 5 subgroups according to the te ... | 2010 | 21033566 |
| crystallization and preliminary crystallographic analysis of achromobacter protease i mutants. | achromobacter protease i (api), a serine protease, shows an order of magnitude higher activity than bovine trypsin. the optimum ph of mutant enzymes with his210 replaced by ser (h210s) and trp169 replaced by phe (w169f) has been shown to shift from approximately ph 9 (wild-type enzyme) to approximately ph 7 while retaining high activity. the mutants were crystallized by the hanging-drop vapour-diffusion technique with 2 m ammonium sulfate as the precipitant. the space group of the w169f mutant c ... | 2010 | 21045314 |
| utilization of phenols and related compounds by achromobacter. | | 1946 | 21065143 |
| an outbreak of achromobacter xylosoxidans associated with ultrasound gel used during transrectal ultrasound guided prostate biopsy. | we describe an outbreak of achromobacter xylosoxidans after transrectal ultrasound guided prostate biopsy at a urology unit at a tertiary care center as well as clinical and microbiological investigation, and intervention. | 2010 | 21074197 |
| [study on gfp labeling of a 2,4-d degrading strain and its detection in a wastewater biotreatment system]. | a 2,4-dichlorophenoxyacetic acid (2,4-d) degrading special bacteria achromobacter sp. was chromosomally labeled with a green fluorescent protein gene (gfp) using a mini-tn7 transposon delivery system. the growth status, fluorescence expression and degradation ability of the strain before and after labeling were compared. methods to quantify the strain in different biotreatment systems (activated sludge or granular sludge system) after inoculation were also investigated. results showed that the l ... | 2010 | 21090306 |
| complete genome sequence of the haloaromatic acid-degrading bacterium achromobacter xylosoxidans a8. | achromobacter xylosoxidans strain a8 was isolated from soil contaminated with polychlorinated biphenyls. it can use 2-chlorobenzoate and 2,5-dichlorobenzoate as sole sources of carbon and energy. this property makes it a good starting microorganism for further development toward a bioremediation tool. the genome of a. xylosoxidans consists of a 7-mb chromosome and two large plasmids (98 kb and 248 kb). besides genes for the utilization of xenobiotic organic substrates, it contains genes associat ... | 2010 | 21097610 |
| characterization of bacterial community inhabiting the surfaces of weathered bricks of nanjing ming city walls. | nanjing ming city wall, one of the important historic heritages in china, has greatly suffered weathering. microbes play an important role in the weathering of historic buildings. however, little is known about the microbial community inhabiting naturally weathered brick minerals and their roles in the mineral weathering. to examine the associations between microorganisms and brick weathering process, we compare the phylogenetic diversity, abundance, community structure, and specific functional ... | 2010 | 21112073 |
| heterotrophic nitrification-aerobic denitrification by novel isolated bacteria. | three novel strains capable of heterotrophic nitrification-aerobic denitrification were isolated from the landfill leachate treatment system. based on their phenotypic and phylogenetic characteristics, the isolates were identified as agrobacterium sp. lad9, achromobacter sp. gad3 and comamonas sp. gad4, respectively. batch tests were carried out to evaluate the growth and the ammonia removal patterns. the maximum growth rates as determined from the growth curve were 0.286, 0.228, and 0.433 h(-1) ... | 2010 | 21113643 |
| cloning of the chlorothalonil-degrading gene cluster and evidence of its horizontal transfer. | strain ochrobactrum lupine tp-d1 was found to degrade chlorothalonil (tpn) to 4-hydroxy-chlorothalonil (tpn-oh). to clone the related degrading gene, genomic library of tp-d1 was constructed using escherichia coli dh10b and two positive clones 889 and 838 were gained. however, no plasmid was detected in clone 889. and in clone 838, a 3494 bp fragment was cloned which contains a 984 bp hydrolytic dehalogenase (chd) gene and a 1926 bp insertion element is-olup. the insertion element contains a tra ... | 2010 | 21127873 |
| biodegradation of endosulfan and endosulfan sulfate by achromobacter xylosoxidans strain c8b in broth medium. | endosulfan is one of the most widely used wide spectrum cyclodiene organochlorine insecticide. in environment, endosulfan can undergo either oxidation or hydrolysis reaction to form endosulfan sulfate and endosulfan diol respectively. endosulfan sulfate is as toxic and as persistent as its parent isomers. in the present study, endosulfan degrading bacteria were isolated from soil through selective enrichment technique using sulfur free medium with endosulfan as sole sulfur source. out of the 8 i ... | 2010 | 21161332 |
| why two are not enough: degradation of p-toluenesulfonate by a bacterial community from a pristine site in moorea, french polynesia. | in previous work, only one culture (strain ta12) from a pristine site was reported to utilize the xenobiotic compound p-toluenesulfonate (tsa) as a sole source of carbon and energy for aerobic growth. 'strain ta12' has now been recognized as a community of three bacteria: achromobacter xylosoxidans ta12-a, ensifer adhaerens ta12-b and pseudomonas nitroreducens ta12-c. achromobacter xylosoxidans ta12-a and e. adhaerens ta12-b were identified as the tsa degraders. these two organisms contain sever ... | 2011 | 21204940 |
| achromobacter xylosoxidans respiratory tract infection in cystic fibrosis patients. | the aims of this study were to evaluate the frequency of achromobacter xylosoxidans infection in a cohort of cystic fibrosis patients, to investigate antimicrobial sensitivity, to establish possible clonal likeness among strains, and to address the clinical impact of this infection or colonization on the general outcome of these patients. the study was undertaken between january 2004 and december 2008 on 300 patients receiving care at the regional cystic fibrosis center of the naples university ... | 2011 | 21279730 |
| biodegradation of phenanthrene using adapted microbial consortium isolated from petrochemical contaminated environment. | in developing countries like india, there are many industrial areas discharging effluent containing large amount of polyaromatic hydrocarbon (pah) which causes hazardous effect on the soil-water environment. the objective of this study was to isolate and characterize high-efficiency pah-degrading microbial consortium from 3 decade old petrochemical refinery field located in nagpur, maharashtra with history of pah disposal. based on biochemical tests and 16s rdna gene sequence analysis the consor ... | 2011 | 21281999 |
| kinetics of carbon sharing in a bacterial consortium revealed by combining stable isotope probing with fluorescence-activated cell sorting. | aims: to determine the kinetics of substrate fluxes in a microbial community in order to elucidate the roles of the community members. methods and results: the kinetics of substrate sharing in a bacterial consortium were measured by a new analytical approach combining immunostaining, stable isotope probing and fluorescence-activated cell sorting (facs). the bacterial consortium, consisting of four strains and growing on 4-chlorosalicylate (4-cs), was pulse-dosed with the degradation intermedia ... | 2011 | 21299770 |
| rapid genotyping of achromobacter xylosoxidans, acinetobacter baumannii, klebsiella pneumoniae, pseudomonas aeruginosa and stenotrophomonas maltophilia isolates using melting curve analysis of rapd-generated dna fragments (mcrapd). | typing of bacteria is important for monitoring newly emerging pathogens and for examining local outbreaks. we evaluated the randomly amplified polymorphic dna technique in combination with melting curve analysis (mcrapd) of the amplified dna fragments to genotype isolates from five gram-negative species, i.e. achromobacter xylosoxidans, acinetobacter baumannii, klebsiella pneumoniae, pseudomonas aeruginosa and stenotrophomonas maltophilia. by determining the melting temperature peaks of the ampl ... | 2011 | 21320595 |
| bacterial community composition characterization of a lead-contaminated microcoleus sp. consortium. | a microcoleus sp. consortium, obtained from the ebro delta microbial mat, was maintained under different conditions including uncontaminated, lead-contaminated, and acidic conditions. | 2011 | 21340467 |
| facultative methylotrophs from the human oral cavity and methylotrophy in strains of gordonia, leifsonia, and microbacterium. | we show that bacteria with methylotrophic potential are ubiquitous in the human mouth microbiota. numerous strains of actinobacteria (brevibacterium, gordonia, leifsonia, microbacterium, micrococcus, rhodococcus) and proteobacteria (achromobacter, klebsiella, methylobacterium, pseudomonas, ralstonia) were isolated, and one strain of each of the eleven genera was studied in detail. these strains expressed enzymes associated with methylotrophic metabolism (methanol, methylamine, and formate dehydr ... | 2011 | 21374057 |
| two unique presentations of achromobacter xylosoxidans infections in clinical settings. | two rare and unique infections of achromobacter xylosoxidans are described. the first case is a novel presentation of acute necrotising pancreatitis leading to a pancreatic pseudocyst, which was treated successfully in an immunocompetent male. the second case describes a local wound infection of metastatic ductal carcinoma of the breast; the patient consequently succumbed from a pre-existing co-morbid condition. vigilant and efficient microbiological workup and surveillance are needed to diagnos ... | 2011 | 21389595 |
| application of a continuously stirred tank bioreactor (cstr) for bioremediation of hydrocarbon-rich industrial wastewater effluents. | a continuously stirred tank bioreactor (cstr) was used to optimize feasible and reliable bioprocess system in order to treat hydrocarbon-rich industrial wastewaters. a successful bioremediation was developed by an efficient acclimatized microbial consortium. after an experimental period of 225 days, the process was shown to be highly efficient in decontaminating the wastewater. the performance of the bioaugmented reactor was demonstrated by the reduction of cod rates up to 95%. the residual tota ... | 2011 | 21419572 |
| diversity of culturable chitinolytic bacteria from rhizospheres of agronomic plants in japan. | a total of 100 isolates of chitinolytic bacteria were obtained from the rhizospheres of various agronomic plants, and the 16s rrna gene sequences of these isolates were determined. phylogenetic analyses revealed that 81 isolates belonged to the classes betaproteobacteria (39 isolates) and gammaproteobacteria (42 isolates). of the remaining 19 isolates, 16 belonged to the phylum firmicutes. clustering analysis identified 6 and 3 operational taxonomic units (otus) in gammaproteobacteria and betapr ... | 2011 | 21487197 |
| visualization and direct counting of individual denitrifying bacterial cells in soil by nirk-targeted direct in situ pcr. | the abundance of denitrifying bacteria in soil has been determined primarily by the conventional most probable number (mpn) method. we have developed a single-cell identification technique that is culture-independent, direct in situ pcr, to enumerate denitrifying bacteria in soils. the specificity of this method was evaluated with six species of denitrifying bacteria using nirk as the target gene; escherichia coli was used as a negative control. almost all (97.3%-100%) of the nirk-type denitrify ... | 2011 | 21487206 |
| identification of achromobacter xylosoxidans by detection of the bla(oxa-114-like) gene intrinsic in this species. | achromobacter xylosoxidans is an emerging pathogen among patients with cystic fibrosis. here we describe a specific pcr for identification of this organism, based on detection of bla(oxa-114-like). comparison of isolates by pulsed-field gel electrophoresis revealed evidence of cross-infection in some cases, but most patients harbored their own strain. | 2011 | 21497041 |
| complicated septic shock caused by achromobacter xylosoxidans bacteremia in a patient with acute lymphoblastic leukaemia. | infections caused by achromobacter xylosoxidans cause significant morbidity and mortality in debilitated individuals. eradication of these infections requires prolonged therapy with antimicrobial agents and removal of any infected central venous catheter. the outcome is usually poor in patients with high risk malignancy, septic complications, and/or multi-organ dysfunction. | 2007 | 21503249 |
| late hematogenous bacterial infections of breast implants: two case reports of unique bacterial infections. | late infections of breast implants are rare occurrences. we present 2 cases of late infections of breast implants, resulting from hematogenous spread from bacterial infection from distant sites. the first case involves a late implant infection following development of a chronic foot sore, caused by achromobacter xylosoxidans, an aerobic pathogen. the second case describes a late infection, after extensive dental treatment, caused by streptococcus viridans, a bacterium that normally lives in clos ... | 2011 | 21508821 |
| revisited distribution of nonfermenting gram-negative bacilli clinical isolates. | nonfermenting gram-negative bacilli (nf-gnb) are ubiquitous environmental opportunistic bacteria frequently misidentified by conventional phenotypic methods. the aim of this study was to determine the distribution of nf-gnb species by 16 s rrna gene sequencing (used as reference method) and to compare performances of biochemical tests and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof-ms). from nine french hospitals, 188 nf-gnb isolates (except p. aerugin ... | 2011 | 21509476 |
| rapid determination of quinoline and 2-hydroxyquinoline in quinoline biodegradation process by tri-wavelength uv/vis spectroscopy. | for the present study, a tri-wavelength uv/vis spectrophotometric method for rapid determination of quinoline (q) and 2-hydroxyquinoline (hq) during q biodegradation was developed. based on the spectral measurements at 289 nm (the isosbestic point of q and hq), 326 and 380 nm, the spectral interference of extracellular polymeric substances (eps) in the process samples could be minimized, and the amounts of q and hq could be simultaneously quantified. our results indicated that the relative stand ... | 2011 | 21558655 |
| achromobacter xylosoxidans infection presenting as a pulmonary nodule mimicking cancer: case report and literature review. | achromobacter xylosoxidans is typically isolated from pulmonary sources, presenting as pneumonia in immunosuppressed individuals. we describe a novel clinical presentation of a. xylosoxidans infection presenting as multiple spiculated, pulmonary nodules mimicking cancer for which the patient underwent a wedge resection of the lung for diagnosis and staging of presumptive cancer. | 2011 | 21593259 |
| culture-independent analysis of bacterial fuel contamination provides insights into the level of concordance with the standard industry practice of aerobic cultivation. | bacterial diversity in contaminated fuels has not been systematically investigated using cultivation-independent methods. the fuel industry relies on phenotypic cultivation-based contaminant identification, which may lack accuracy and neglect difficult-to-culture taxa. using industry practice aerobic cultivation, 16s rrna gene sequencing and strain genotyping, a collection of 152 unique contaminant isolates from 54 fuel samples was assembled, and demonstrated a dominance of pseudomonas (21%), bu ... | 2011 | 21602386 |
| biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium: a laboratory study. | this article presents a study of the efficiency and degradation pattern of samples of petroleum sludge and polluted sandy soil from an oil refinery. a bacterial consortium, consisting of strains from the genera pseudomonas, achromobacter, bacillus and micromonospora, was isolated from a petroleum sludge sample and characterized. the addition of nitrogen and phosphorus nutrients and a chemical surfactant to both the samples and bioaugmentation to the soil sample were applied under laboratory cond ... | 2011 | 21604191 |
| intragenomic and intraspecific heterogeneity of the 16s rrna gene in seven bacterial species from the respiratory tract of cystic fibrosis patients assessed by pcr-temporal temperature gel electrophoresis. | 16s rrna gene-based cultivation-independent methods are increasingly used to study the diversity of microbiota during health and disease. one bias of these methods is the variability of 16s rrna gene that may exist among strains of a same species (intraspecific heterogeneity) or between rrs copies in a genome (intragenomic heterogeneity). we evaluated the level of intraspecific and intragenomic 16s rdna variability in seven species frequently encountered in respiratory tract samples in cystic fi ... | 2011 | 21621347 |
| a case of meningitis due to achromobacter xylosoxidans denitrificans 60 years after a cranial trauma. | background: achromobacter xylosoxidans (ax) is a non-fermentative aerobic gram-negative bacillus. it is an opportunistic pathogen and the causative agent of various infections. we report an original case of late posttraumatic meningitis due to ax denitrificans.<br /> case report: an 83-year-old man was hospitalized for acute headache, nausea and vomiting. the emergency brain computer tomography (ct) scan did not reveal any anomaly. in his medical history, there was an auditory injury due to a cr ... | 2011 | 21629191 |
| fatal catheter-related bacteremia due to alcaligenes (achromobacter) xylosoxidans in a hemodialysis patient. | | 2011 | 21637989 |
| new approaches to identification and activity estimation of glyphosate degradation enzymes. | we propose a new set of approaches, which allow identifying the primary enzymes of glyphosate (n-phosphonomethyl-glycine) attack, measuring their activities, and quantitative analysis of glyphosate degradation in vivo and in vitro. using the developed approach we show that glyphosate degradation can follow different pathways depending on physiological characteristics of metabolizing strains: in ochrobactrum anthropi gpk3 the initial cleavage reaction is catalyzed by glyphosate-oxidoreductase wit ... | 2011 | 21639854 |
| a comparative study of biodegradation of vinyl acetate by environmental strains. | four gram-negative strains, e3_2001, ec1_2004, ec3_3502 and ec2_3502, previously isolated from soil samples, were subjected to comparative studies in order to select the best vinyl acetate degrader for waste gas treatment. comparison of biochemical and physiological tests as well as the results of fatty acids analyses were comparable with the results of 16s rrna gene sequence analyses. the isolated strains were identified as pseudomonas putida ec3_2001, pseudomonas putida ec1_2004, achromobacter ... | 2011 | 21654921 |
| aerobic degradation of 2,4-dinitrotoluene by individual bacterial strains and defined mixed population in submerged cultures. | the degradation efficiencies of isomeric mononitrotoluenes (2- and 4-nts) and dinitrotoluenes (2,4-dnt and 2,6-dnt) by either individual bacterial strains (bacillus cereus ndt4, pseudomonas putida ndt1, pseudomonas fluorescens ndt2, and achromobacter sp. ndt3) or their mixture were compared in submerged batch cultivations. the mixed culture degraded 2,4-dnt nearly 50 times faster than any of the individual strains. the mixed culture also demonstrated significantly shorter lag periods in 2,4-dnt ... | 2011 | 21665364 |
| simultaneous hydrocarbon biodegradation and biosurfactant production by oilfield-selected bacteria. | aims:ôçé to study the bacterial diversity associated with hydrocarbon biodegradation potentiality and biosurfactant production of tunisian oilfields bacteria. methods and results:ôçé eight tunisian hydrocarbonoclastic oilfields bacteria have been isolated and selected for further characterization studies. phylogenetic analysis revealed that three thermophilic strains belonged to the genera geobacillus, bacillus and brevibacillus, and that five mesophilic strains belonged to the genera pseudomona ... | 2011 | 21668593 |
| first description of a rnd-type multidrug efflux pump in achromobacter xylosoxidans: axyabm. | achromobacter xylosoxidans is an emerging pathogen in cystic fibrosis patients. the multidrug resistance of these bacteria remains poorly understood. we have characterized in a clinical strain the first resistance-nodulation-cell division (rnd)-type multidrug efflux pump in this species: axyabm. the inactivation of the transporter component axyb gene led to decreased minimum inhibitory concentrations (mics) of cephalosporins (except cefepime), aztreonam, nalidixic acid, fluoroquinolones and chlo ... | 2011 | 21807978 |
| distribution of glyphosate and methylphosphonate catabolism systems in soil bacteria ochrobactrum anthropi and achromobacter sp. | bacterial strains capable of utilizing methylphosphonic acid (mp) or glyphosate (gp) as the sole sources of phosphorus were isolated from soils contaminated with these organophosphonates. the strains isolated from mp-contaminated soils grew on mp and failed to grow on gp. one group of the isolates from gp-contaminated soils grew only on mp, while the other one grew on mp and gp. strains achromobacter sp. mps 12 (vkm b-2694), mp degraders group, and ochrobactrum anthropi gpk 3 (vkm b-2554d), gp d ... | 2011 | 21789492 |
| marked increase in incidence of achromobacter xylosoxidans infections caused by sporadic acquisition from the environment. | background: an increased incidence of achromobacter xylosoxidans infections has been observed at the cystic fibrosis centre at aarhus university hospital, as the proportion of patients colonised with a. xylosoxidans increased from 6 to 10% from 2005 to 2009. methods: pulsed field gel electrophoresis (pfge) was used to type isolates of a. xylosoxidans. results: four patients infected for 2-7years were part of a larger epidemic spread involving both danish cf centres, while 11 patients carried str ... | 2011 | 21835703 |
| enrichment of co2-fixing bacteria in cylinder-type electrochemical bioreactor with built-in anode compartment. | bacterial assimilation of co2 into stable biomolecules using electrochemical reducing power may be an effective method to reduce atmospheric co2 without fossil fuel combustion. for the enrichment of the co2-fixing bacteria using electrochemical reducing power as an energy source, a cylinder-type electrochemical bioreactor with a built-in anode compartment was developed. a graphite felt cathode modified with neutral red (nr-graphite cathode) was used as a solid electron mediator to induce bacteri ... | 2011 | 21715965 |
| the electron transfer complex between nitrous oxide reductase and its electron donors. | identifying redox partners and the interaction surfaces is crucial for fully understanding electron flow in a respiratory chain. in this study, we focused on the interaction of nitrous oxide reductase (n(2)or), which catalyzes the final step in bacterial denitrification, with its physiological electron donor, either a c-type cytochrome or a type 1 copper protein. the comparison between the interaction of n(2)or from three different microorganisms, pseudomonas nautica, paracoccus denitrificans, a ... | 2011 | 21739254 |
| Could petroleum biodegradation be a joint achievement of aerobic and anaerobic microrganisms in deep sea reservoirs? | ABSTRACT: Several studies suggest that petroleum biodegradation can be achieved by either aerobic or anaerobic microorganisms, depending on oxygen input or other electron acceptors and appropriate nutrients. Evidence from in vitro experiments with samples of petroleum formation water and oils from Pampo Field indicate that petroleum biodegradation is more likely to be a joint achievement of both aerobic and anaerobic bacterial consortia, refining our previous observations of aerobic degradation. ... | 2011 | 22196374 |
| Decolorizing activity of malachite green and its mechanisms involved in dye biodegradation by Achromobacter xylosoxidans MG1. | An Achromobacter xylosoxidans MG1 strainisolated from the effluent treatment plant of a textile and dyeing factory from Yunnan Province in China was found capable of decolorizing the malachite green dye at a high efficacy. Strain MG1 reduced 86% malachite green at the concentration of 2,000 mg/l within 1 h, representing a greater ability for decolorizing and a higher tolerance of this compound than all previously reported bacteria. Color removal was optimal at pH 6 and 38°C. Further experimental ... | 2011 | 21865764 |
| Oxidation of arsenite by two ß-proteobacteria isolated from soil. | Two heterotrophic As(III)-oxidizing bacteria, SPB-24 and SPB-31 were isolated from garden soil. Based on 16S rRNA gene sequence analysis, strain SPB-24 was closely related to genus Bordetella, and strain SPB-31 was most closely related to genus Achromobacter. Both strains exhibited high As(III) (15 mM for SPB-24 and 40 mM for SPB-31) and As(V) (>300 mM for both strains) resistance. Both strains oxidized 5 mM As(III) in minimal medium with oxidation rate of 554 and 558 µM h(-1) for SPB-24 and SPB ... | 2011 | 21983709 |
| achromobacter xylosoxidans: characterization of strains in brazilian cystic fibrosis patients. | we investigated the possibility of cross-infection among cystic fibrosis patients in two brazilian reference centers. achromobacter xylosoxidans isolates (n = 122) were recovered over a 5-year period from 39 patients. isolates were genetically heterogeneous, but one genotype was present in 56% of the patients, suggesting that cross-infection may have occurred. | 2011 | 21849686 |
| enhanced chrysene degradation by halotolerant achromobacter xylosoxidans using response surface methodology. | degradation of chrysene, a four ring high molecular weight (hmw) polycyclic aromatic hydrocarbon (pah) is of intense environmental interest, being carcinogenic, teratogenic and mutagenic. multiple pah degrading halotolerant achromobacter xylosoxidans was isolated from crude oil polluted saline site. response surface methodology (rsm) using central composite design (ccd) of bushnell-haas medium components was successfully employed for optimization resulting 40.79% chrysene degradation on 4th day. ... | 2011 | 21855331 |
| challenges of sulfur sad phasing as a routine method in macromolecular crystallography. | the sulfur sad phasing method allows the determination of protein structures de novo without reference to derivatives such as se-methionine. the feasibility for routine automated sulfur sad phasing using a number of current protein crystallography beamlines at several synchrotrons was examined using crystals of trimeric achromobacter cycloclastes nitrite reductase (acnir), which contains a near average proportion of sulfur-containing residues and two cu atoms per subunit. experiments using x-ray ... | 2012 | 22186640 |
| fluorescence lifetime analysis of nitrite reductase from alcaligenes xylosoxidans at the single-molecule level reveals the enzyme mechanism. | | 2011 | 21922585 |
| biodiversity characterization of cellulolytic bacteria present on native chaco soil by comparison of ribosomal rna genes. | sequence analysis of the 16s ribosomal rna gene was used to study bacterial diversity of a pristine forest soil and of two cultures of the same soil enriched with cellulolytic bacteria. our analysis revealed high bacterial diversity in the native soil sample, evidencing at least 10 phyla, in which actinobacteria, proteobacteria and acidobacteria accounted for more than 76% of all sequences. in both enriched samples, members of proteobacteria were the most frequently represented. the majority of ... | 2011 | 22202170 |
| Achromobacter xylosoxidans mesh related infection: A case of delayed diagnosis and management. | We present the first case of mesh related infection caused by Achromobacter xylosoxidans after ventral hernia repair. After repair of a small paraumbilical hernia, the postoperative course was complicated by persistent discharging sinuses despite the removal of underlying polypropylene mesh. Removal of an intrabdominal omental inflammatory mass containing pus that showed growth of A. xylosoxidans led to the resolution of all the symptoms. | 2011 | 21959012 |
| the effect of carbon monoxide on bacterial growth. | growth rate studies were conducted with pseudomonas aeruginosa, pseudomonas fluorescens, escherichia coli and achromabacter under atmospheres containing up to thirty per cent (v/v) carbon monoxide. it was found that atmospheres containing carbon monoxide have a selective action on the type of organism that grows in a mixed culture. studies with pure cultures demonstrated that carbon monoxide has no effect on the growth of p. aeruginosa, inhibits the growth rate of e. coli by amounts proportional ... | 1981 | 22056030 |
| Distribution of microbial biomass and the potential for anaerobic respiration in Hanford Site 300 Area subsurface sediment. | Subsurface sediments were recovered from a 52 m deep borehole cored in the 300 Area of the Hanford Site in southeastern Washington State to assess the potential for biogeochemical transformation of radionuclide contaminants. Microbial analyses were made on 17 sediment samples traversing multiple geological units: the oxic coarse-grained Hanford formation (9-17.4 m), the oxic fine-grained upper Ringold Formation (17.7-18.1 m), and the reduced Ringold Formation (18.3-52m). Microbial biomass (measu ... | 2011 | 22138990 |