immune response to campylobacter jejuni in a rural community in thailand. | we studied the prevalence of antibodies to campylobacter jejuni in serum from healthy thai villagers by using enzyme-linked immunosorbent assays with c. jejuni surface proteins as antigens. levels of c. jejuni-specific iga rose progressively through life, igg peaked in the second year of life and then fell, and igm peaked during late childhood and the teenage years. these findings confirm results observed in bangladeshi children, and they suggest there is intense early exposure and continued exp ... | 1986 | 3944480 |
campylobacter jejuni diarrhea in a 3-day-old male neonate. | | 1986 | 3946343 |
multiple angiomatous proliferations of ileal stoma following campylobacter enteritis. effect of laser photocoagulation. | multiple polypoid sessile tumors arose at the tip of the ileal stoma of a 37-year-old man, who previously underwent proctocolectomy for ulcerative colitis. the lesions developed shortly after a well-established gastrointestinal infection with campylobacter jejuni with mucosal ulceration. the histological and ultrastructural examination showed angiomatous proliferations, reminiscent of pyogenic granuloma. the destruction of the tumors by laser photocoagulation was followed by superficial ulcerati ... | 1986 | 3948633 |
distribution of sero-biotypes of campylobacter jejuni and c. coli isolated from paediatric patients. | during a one-year period, 258 isolates of campylobacter jejuni and c. coli were obtained from children with gastroenteritis or bacteraemia at the red cross children's hospital, cape town, south africa. these isolates were biotyped by hippurate hydrolysis, h2s production and tolerance to 2,3,5-triphenyltetrazolium chloride (ttc). our study indicated that 95.4% of the isolates were c. jejuni biotype 1, 1.5% were c. jejuni biotype 2 and 3.1% were c. coli; 70% of the isolates were resistant to ttc. ... | 1986 | 3950960 |
a survey of campylobacter and other bacterial contaminants of pre-market chicken and retail poultry and meats, king county, washington. | as part of a larger study to determine the flow of campylobacter and salmonella from food animals to humans during 1982-83, 1,936 swabs were collected for bacteriologic study from pre-market chickens, retail poultry, and other retail meats as well as from equipment and work surfaces used to process such foods. of the 297 samples collected in a poultry processing plant, 56.6 per cent were positive for campylobacter jejuni/coli (cjc), as were 23.1 per cent of the 862 retail chicken, and 17.2 per c ... | 1986 | 3953916 |
the role of poultry and meats in the etiology of campylobacter jejuni/coli enteritis. | to determine the role of meats as possible sources of infection leading to campylobacter jejuni/coli (cjc) enteritis, 218 cases and 526 controls were selected from the king county group health cooperative (ghc) population from april 1982 through september 1983. all subjects were interviewed regarding food consumption one week prior to case onset. consumption of chicken and cornish game hen were both associated with more than a doubling of the risk of cjc enteritis: for chicken (relative risk = 2 ... | 1986 | 3953917 |
epidemic campylobacteriosis associated with a community water supply. | in may 1983, an estimated 865 cases of epidemic gastrointestinal disease occurred in greenville, florida. surveillance of pharmacy sales of antidiarrheal medicines suggested that the outbreak was confined to greenville and its immediate vicinity. surveys demonstrated that the gastrointestinal illness attack rates inside and outside the city limits were 56 per cent (72/128) and 9 per cent (7/77), respectively (relative risk (rr) = 6.2); consumption of city water was associated with illness (rr = ... | 1986 | 3953920 |
epidemiologic study of campylobacteriosis in iowa cattle and the possible role of unpasteurized milk as a vehicle of infection. | bile samples were collected from 477 iowa dairy cows and were cultured for thermophilic campylobacters. the prevalence of thermophilic campylobacters in the bile was 15.5%. campylobacter jejuni and c coli from dairy cattle, chickens, pigs, sheep, and human beings were serotyped to develop host-species profiles. human and cattle serologic profiles were the most similar, and human and chicken profiles shared several similarities. epidemiologic data from 168 human cases of campylobacteriosis indica ... | 1986 | 3954200 |
inactivation of campylobacter jejuni by chlorine and monochloramine. | campylobacter jejuni and closely related organisms are important bacterial causes of acute diarrheal illness in the united states. both endemic and epidemic infections have been associated with consuming untreated or improperly treated surface water. we compared susceptibility of three c. jejuni strains and escherichia coli atcc 11229 with standard procedures used to disinfect water. inactivation of bacterial preparations with 0.1 mg of chlorine and 1.0 mg of monochloramine per liter was determi ... | 1986 | 3954344 |
methods for isolating campylobacter jejuni from low-turbidity water. | membrane filtration methods were developed and evaluated for the quantitative recovery of campylobacter jejuni from environmental waters of low turbidity. the best procedure studied involved passaging the test water through a filter (pore size, 0.45 micron) and plating it facedown on campylobacter-selective agar. the filter was removed after overnight incubation, and the plate was streaked for isolation and then reincubated. this method, with or without prefiltration through 5.0- and 0.6-micron- ... | 1986 | 3954345 |
isolation of campylobacter jejuni from retail mushrooms. | campylobacter jejuni was isolated from 3 (1.5%) of 200 retail, polyvinyl chloride film-wrapped, fresh mushrooms. these results indicate that fresh mushrooms may indeed be a source of c. jejuni and support previously reported epidemiological data (seattle-king county department of public health, surveillance of the flow of salmonella and campylobacter in a community, 1984) which revealed an an elevated relative risk of developing campylobacter enteritis in individuals who consume mushrooms. | 1986 | 3954354 |
campylobacter jejuni and infantile traveller's diarrhoea. | | 1986 | 3956542 |
evaluation of a blood-free, charcoal-based, selective medium for the isolation of campylobacter organisms from feces. | a blood-free, charcoal-based selective medium (csm) consisting of a columbia agar base, activated charcoal (4 g/liter), hematin (0.032 g/liter), sodium pyruvate (0.1 g/liter), cefoperazone (32 mg/liter), vancomycin (20 mg/liter), and cycloheximide (100 mg/liter) supported the growth of campylobacter jejuni and c. coli with colony counts equivalent to those obtained on antibiotic-free horse blood agar. csm was compared to skirrow medium (skm) for the recovery of c. jejuni and c. coli from stools ... | 1986 | 3958143 |
distribution and serotypes of campylobacter jejuni and campylobacter coli in enteric campylobacter strains isolated from children in the central african republic. | one hundred eighty-five enteric campylobacter strains isolated from diarrheic or healthy children in bangui (central african republic) were studied to determine their species and serotypes. c. coli was identified in 38.9% of all strains and in 43.9% of strains from diarrheic children. by the hemagglutination technique for heat-stable antigens, 73.5% of the strains could be serotyped. of the typeable strains, 75% were distributed among 13 more frequent serotypes. c. coli serotype pen 37,56 was th ... | 1986 | 3958146 |
colonization of syrian hamsters with streptomycin resistant campylobacter jejuni. | a streptomycin resistant campylobacter jejuni inoculated per os into two populations of syrian hamsters (one endemically harboring c. jejuni, the other free of c. jejuni) established chronic colonization of the organism in both groups. diet, steroid administration, age of hamsters or prior exposure to c. jejuni did not appreciably alter incidence of diarrhea or colonization of c. jejuni. the majority of hamsters sampled during the course of the experiment (1 to 22 weeks) shed streptomycin resist ... | 1986 | 3959531 |
[campylobacter jejuni. infections in northern norway in 1980-84]. | | 1986 | 3961747 |
campylobacter jejuni septicemia--epidemiology, clinical features and outcome. | in 33 cases of campylobacter jejuni septicemia, the disease was more common at the extremes of age: infants made up a third of the reported cases while 24% of patients were older than 50 years. fever was noted in more than 80% of patients and chills in about a fourth. enteritis was present in 70% of cases, and the gastrointestinal tract was the principal source of septicemia. half of the patients did not have significant underlying disease but were at extremes of age, which may reflect relative ... | 1986 | 3962297 |
[suitability of various culture media for medium-term storage of campylobacter jejuni/coli strains]. | | 1986 | 3962367 |
etiology of acute infectious diarrhea in a highly industrialized area of switzerland. | during an 18-mo period between 1981 and 1982, a prospective study was conducted in 119 adult patients with acute diarrhea. a diarrhea-inducing microorganism or toxin could be identified in 38.7% of the patients. salmonella sp and campylobacter jejuni were the leading agents that caused diarrheal illness in 25% of the investigated population. clostridium difficile was found in 6%, mainly after previous antibiotic therapy. rotavirus was rarely isolated and enterotoxigenic escherichia coli were not ... | 1985 | 3964774 |
lipopolysaccharide characteristics of pathogenic campylobacters. | most campylobacter jejuni strains are sensitive and most campylobacter fetus strains are resistant to the bactericidal activity in normal human serum. we purified lipopolysaccharides from campylobacter strains to determine whether their composition and structure relate to serum susceptibility. the lipopolysaccharide of two serum-sensitive strains was best isolated by the galanos procedure, but for two serum-resistant strains a cold-ethanol extraction was optimal. for each lipopolysaccharide prep ... | 1985 | 3967920 |
in vitro antimicrobial susceptibility, plasmid analysis, and serotyping of epidemic-associated campylobacter jejuni. | campylobacter jejuni strains from 11 outbreaks were characterized by antimicrobial susceptibility, plasmid profile, and serotyping by the methods of lior et al. and penner and hennessy. all 31 strains were susceptible to erythromycin, clindamycin, chloramphenicol, kanamycin, tobramycin, streptomycin, and gentamicin. a total of 21 strains from nine outbreaks were resistant to one or more of the following antimicrobial agents: tetracycline, metronidazole, ampicillin, or carbenicillin. of the 31 st ... | 1985 | 3968203 |
susceptibility of campylobacter isolates to the bactericidal activity of human serum. | although campylobacter jejuni and related thermophilic organisms are more common human pathogens than are campylobacter fetus, most bloodstream or systemic isolates are c. fetus. to understand the pathophysiology related to this observation, the authors studied susceptibility to the bactericidal activity of normal human serum of campylobacter coli, c. jejuni, and c. fetus isolates from feces and blood. in standardized assays, 10 of 15 c. jejuni and related isolates showed 90% kill (mean, 90.6% + ... | 1985 | 3968449 |
campylobacter jejuni-specific serum antibodies are elevated in healthy bangladeshi children. | in bangladesh and other developing countries, isolation of campylobacter jejuni is common in healthy children, and the illness/infection ratio falls with age. to determine whether specific serum antibodies correlate with this phenomenon, using an enzyme-linked immunosorbent assay, we studied sera from 93 healthy bangladeshi children and 121 healthy u.s. children under 15 years of age. for each age group (less than 1, 2 to 4, and 5 to 14 years) studied, specific serum antibody levels were signifi ... | 1985 | 3972984 |
illness associated with campylobacter laridis, a newly recognized campylobacter species. | campylobacter laridis, a recently described thermophilic campylobacter species found principally in seagulls, has not previously been linked to illness in humans. six clinical isolates of this species were referred to the national campylobacter reference laboratory in 1982 and 1983. each isolate was confirmed by biochemical characterization and by dna relatedness studies. the six isolates were obtained during an illness: enteritis in four, severe crampy abdominal pain in one, and terminal bacter ... | 1985 | 3972989 |
comparison of basal media for culturing campylobacter jejuni and campylobacter coli. | four strains of campylobacter jejuni and four strains of campylobacter coli were used to compare the quantitative growth of campylobacter cells on blood agar base no. 2 (oxoid), brucella agar (bbl microbiology systems and difco laboratories), campylobacter agar base (difco), columbia blood agar base (difco and oxoid), and mueller-hinton agar (difco and oxoid). columbia blood agar base and blood agar base no. 2 were inhibitory to most of the strains tested, as evidenced by reduced (10- to 1,000-f ... | 1985 | 3972990 |
the virulence of clinical and environmental isolates of campylobacter jejuni. | the virulence of campylobacter jejuni and c. coli isolated from various water sources was compared with that of clinical strains by in vitro assays of adhesion, invasion and cytotoxicity to hela cells. variation in degree of attachment was observed, but this did not appear to be related to strain source, however, water strains were less invasive and less cytotoxic to hela cells than clinical strains as shown by immunofluorescence and electron microscopy. these differences were particularly evide ... | 1985 | 3973380 |
a one-year survey of campylobacter enteritis and other forms of bacterial diarrhoea in hong kong. | the following enteropathogens were isolated from the faeces of 769 (10.2%) of 7,545 patients of whom 5,704 had diarrhoea or abdominal pain, attending a teaching hospital in hong kong during one year: salmonellae 458 (6.1%); vibrio parahaemolyticus 125 (1.7%); campylobacters 108 (1.4%); shigellae 83 (1.1%); others 19 (0.3%). further identification of the campylobacter isolates showed that 63 (58%) were campylobacter jejuni biotype 1, 44 (41%) were c. coli and only one was c. jejuni biotype 2. sev ... | 1985 | 3973381 |
appearance in patients' sera of antibodies against purified antigen of campylobacter jejuni and its relation to the bacterium-excreting period. | | 1985 | 3973420 |
the prevalence of campylobacter jejuni in random-source cats used in biomedical research. | | 1985 | 3973421 |
campylobacter jejuni mastitis in a cow: a zoonosis-related incident. | | 1985 | 3976151 |
prevalence of campylobacter jejuni and salmonella during pig slaughtering. | it was found that 79% of healthy pigs, slaughtered in three different slaughterhouses in the netherlands, were intestinal carriers of campylobacter jejuni (mean number 4000 cfu per g), and 21% of the same pigs had salmonella in the intestinal tract (mean number 10 cfu per g). immediately after slaughter, campylobacter was swabbed from 9% of the carcasses and salmonella from 13%. it is concluded from these data that most of the contamination on carcasses does not originate directly from the intes ... | 1985 | 3976157 |
[isolation of campylobacter jejuni and campylobacter coli in fecal samples of healthy slaughter swine depending on the season]. | | 1985 | 3976311 |
campylobacter jejuni-associated hemolytic-uremic syndrome: a possible role for endotoxin. | | 1985 | 3977526 |
intestinal parasites and bacteria in aboriginal children in south west australia. | in a study of intestinal parasites in 697 aboriginal children under the age of 6 years in south-west australia, giardia lamblia was recorded in 26% and hymenolepis nana in 13.9%. g. lamblia infections occurred above 4 months of age, and h. nana infections above 18 months of age. there was a close correlation between infection with these two species (chi 2, p less than 0.001). campylobacter jejuni, the predominant bacterial species recorded, was isolated in 2.3%, and was related to bowel symptoms ... | 1985 | 3977790 |
use of auxotyping for epidemiological studies of campylobacter jejuni and campylobacter coli infections. | a chemically defined medium developed for neisseria gonorrhoeae was modified to support the growth of campylobacter jejuni and campylobacter coli. a total of 76 isolates of c. jejuni and 14 isolates of c. coli were tested on this medium, which was designated campylobacter defined medium (cdm), over a 3-month period. although none of the c. coli isolates appeared to require amino acids, 51% of the c. jejuni tested required one and 7% required multiple amino acids for growth. an analysis of isolat ... | 1985 | 3988340 |
phagocytosis of campylobacter jejuni and its intracellular survival in mononuclear phagocytes. | in vitro phagocytosis and intracellular survival of campylobacter jejuni strain 2964 in mononuclear phagocytes were studied. the following three types of mononuclear phagocytes were used: a j774g8 peritoneal macrophage line derived from balb/c mice, resident balb/c peritoneal macrophages, and human peripheral blood monocytes. when c. jejuni and mononuclear phagocytes were combined at a ratio of 75:1, light microscopy, fluorescent microscopy, and electron microscopy all indicated that c. jejuni c ... | 1985 | 3988342 |
evidence of udder excretion of campylobacter jejuni as the cause of milk-borne campylobacter outbreak. | we describe a community outbreak of campylobacter enteritis associated with the consumption of untreated milk, apparently contaminated by two cows with campylobacter mastitis. the outbreak occurred in two phases. strains of campylobacter jejuni of the penner serogroup complex 4, 13, 16, 50 and preston biotype code 6100 were isolated from patients in both episodes and from the faeces of the cattle, milk filters, bulk milk and retail milk. milk samples from two of 40 milking cows were found to con ... | 1985 | 3989284 |
campylobacter jejuni isolated from a rectal biopsy specimen. | | 1985 | 3989330 |
fatalities associated with campylobacter jejuni infections. | although campylobacter jejuni is now recognized as a common cause of gastroenteritis, fatalities associated with this infection in the united states have not been previously reported. two fatalities associated with c jejuni infections occurred over a two-year period in the denver metropolitan area. the first case was in a previously healthy 26-year-old woman who died following a two-day diarrheal illness. the second case was in a 69-year-old diabetic woman who died 19 hours after developing a ga ... | 1985 | 3989964 |
[spontaneous peritonitis caused by campylobacter jejuni in a cirrhotic patient]. | | 1985 | 3990397 |
[season-related incidence of campylobacter jejuni and campylobacter coli in fecal samples of healthy slaughter cattle]. | | 1985 | 3993275 |
estimation of campylobacter spp. in broth culture by bioluminescence assay of atp. | the luciferin-luciferase bioluminescence reaction was used to estimate cell numbers of campylobacter jejuni and campylobacter coli in broth cultures based on a linear relationship between cell numbers (in excess of 10(4) to 10(5] and atp levels. the sensitivity was lower than that obtained with escherichia coli. the calculated amount of intracellular atp per cell of c. jejuni and c. coli ranged from 1.7 to 2.1 fg. | 1985 | 3994377 |
[epidemiologic studies of enteritis caused by campylobacter jejuni. iv. a study of the principal reservoir hosts]. | | 1985 | 3995570 |
typing of heat-stable and heat-labile antigens of campylobacter jejuni and campylobacter coli by coagglutination. | a coagglutination system has been devised for typing heat-stable and heat-labile antigens of campylobacter jejuni and c. coli. the use of protein a-positive staphylococcus aureus cells carrying campylobacter sp. serotype antibody and the treatment of campylobacter sp. cells with dnase in the antigen suspension permitted rapid and specific coagglutination of rough (autoagglutinable) as well as smooth cultures. cells of s. aureus were sensitized with campylobacter sp. serotype antisera. four to fi ... | 1985 | 3998098 |
cadmium chloride susceptibility, a characteristic of campylobacter spp. | we report a simple diagnostic characteristic useful in the presumptive identification of campylobacter jejuni and campylobacter coli. filter paper disks impregnated with cadmium chloride were placed on streaked agar medium. zones of growth inhibition for campylobacter spp. occurred at 1.25 micrograms per disk. other enteropathogens (salmonella spp., shigella spp., vibrio cholerae, vibrio parahaemolyticus, escherichia coli, and yersinia enterocolitica) were resistant to at least 40 micrograms per ... | 1985 | 3998099 |
campylobacter infection in domestic dogs. | campylobacter jejuni, a recently recognised human enteric pathogen, was recovered from the faeces of 21.7 per cent of domestic dogs with diarrhoea as compared with only 3.1 per cent of normal healthy dogs. the recovery rate from non-diarrhoeic, but unhealthy, domestic dogs was 6.7 per cent. the differences in the incidence of c jejuni between breeds was not statistically significant. the majority of isolations were from puppies between birth and six months old. the incidence of c jejuni in domes ... | 1985 | 4002595 |
campylobacter jejuni infections on college campuses: a national survey. | we surveyed 303 colleges and universities to determine the frequency of isolations of campylobacter jejuni and other enteric bacterial pathogens. campylobacter cultures could be obtained at 74 per cent; 10 per cent of stool cultures yielded campylobacter, which was isolated 10 and 46 times more frequently than salmonella and shigella, respectively. the incidence of diagnosed campylobacter infections was 1.3 per 10,000 enrolled students per month. intercollegiate variation was not explained by di ... | 1985 | 4003634 |
serotyping of and hippurate hydrolysis by campylobacter jejuni isolates from human patients, poultry and pigs in the netherlands. | three hundred strains of campylobacter jejuni, isolated from human patients, poultry and pigs were serotyped according to the penner-lauwers system and furthermore tested for hippurate hydrolysis. serotyping showed a close relationship between human and chicken strains, whereas there was little relationship between human and pig strains. a similar conclusion was drawn from the results of the hippurate hydrolysis test: 86% of human and 94% of poultry strains were positive, whereas 91% of pig stra ... | 1985 | 4004203 |
alterations of myoelectric activity associated with campylobacter jejuni and its cell-free filtrate in the small intestine of rabbits. | we evaluated the effects of a culture of campylobacter jejuni and its cell-free filtrate on myoelectric activity of isolated ileal segments in new zealand white rabbits. hematoxylin and eosin staining and scanning electron microscopy were used to assess the association between histologic changes and alterations in intestinal myoelectric activity. a culture of c. jejuni was shown to cause a significant increase in repetitive bursts of action potentials (rbaps) (6.9 +/- 1.2 rbap/h; p less than 0.0 ... | 1985 | 4007424 |
acute polyneuritis with cranial nerve involvement following campylobacter jejuni infection. | | 1985 | 4009198 |
serotyping of campylobacter jejuni isolated from sporadic cases and outbreaks in northern norway. | in the northern regions of norway, 123 cases of campylobacter jejuni gastroenteritis were either sporadic infections or due to five separate outbreaks during 1980-83. using a serotyping scheme based on thermostable antigens and the passive haemagglutination technique, 92 isolates were serotyped. excluding duplicates from the outbreaks, the serotype distribution based on 79 isolates showed that serotypes 6, 7 and 12, 40 were the most frequent in occurrence. each included 11 (13.9%) of the isolate ... | 1985 | 4013744 |
antigenicity of lipopolysaccharides from campylobacter jejuni and campylobacter coli in passive haemagglutination tests and enzyme-linked immunosorbent assays. | passive haemagglutination tests and elisa were used to study the serological activity of homologous and heterologous rabbit antisera against lps prepared from various strains of c. jejuni/coli. in both test systems the homologous antisera exhibited serological activity against lps. the heterologous antisera showed some degree of intra- and inter- species cross-reactivity. the cross-reacting was most pronounced in the elisa. erythrocytes sensitized with untreated lps gave higher antibody-titres t ... | 1985 | 4013751 |
evaluation and diagnosis of acute infectious diarrhea. | the appropriate approach to the diagnosis and management of acute infectious diarrhea is determined by the frequency and setting of the illness, the recognizable causes or syndromes, the cost and yield of available diagnostic tests, and the treatability of the disease. acute diarrhea affects everyone throughout the world from one to more than six times each year, depending on age, location, and living conditions. the range of identifiable viral, bacterial, and parasitic etiologies is great, and ... | 1985 | 4014291 |
laboratory infection of chicken eggs with campylobacter jejuni by using temperature or pressure differentials. | fertile chicken eggs were infected in our laboratory with campylobacter jejuni suspensions by using temperature or pressure differential methods of inoculation. after 2 days of incubation, over 90% of the eggs carried c. jejuni when iron was present in the inoculum. this percentage declined rapidly until by day 8, less than 10% of the eggs were detectably infected. however, up to 11% of hatched, healthy chicks carried c. jejuni in their intestinal tracts. the isolated organisms were of the same ... | 1985 | 4015086 |
serotyping of campylobacter jejuni, campylobacter coli, and campylobacter laridis from domestic and wild animals. | by using 50 unabsorbed antisera, we were able to serotype 272 (65.7%) of 414 thermotolerant campylobacters from wild and domestic animals, on the basis of heat-stable antigens identified by means of passive hemagglutination. forty-two serotypes were recognized. the pattern of serotypes detected in the various animal species was compared to human clinical isolates by using the czekanowski index (proportional similarity index). the highest degree of similarity to the clinical isolates was observed ... | 1985 | 4015088 |
gastroenteritis due to campylobacter jejuni in lagos, nigeria. | | 1985 | 4016913 |
development of a bacteriophage typing system for campylobacter jejuni and campylobacter coli. | a bacteriophage typing system for campylobacter jejuni and campylobacter coli was developed with phages isolated from poultry feces. data for phage selection were generated from a set of isolates of c. jejuni and c. coli from humans in illinois. selection of 14 phages from the 47 phages available was assisted by determination of the sneath-jaccard similarity coefficients and subsequent unweighted pair-group arithmetic averaging cluster analysis. the typing set was reproducible and stable in the ... | 1985 | 4019737 |
biotypes and serotypes of thermophilic campylobacters isolated from cattle, sheep and pig offal and other red meats. | in this study we examined 730 faecal samples of offal (mainly liver), mince-meat and sausage meat collected from abattoirs and retail butchers' shops for campylobacters. campylobacter jejuni or c. coli were isolated from 30.6, 10.5 and 6% of sheep, cattle and pig offal samples respectively. specimens collected from abattoirs were, in general, more often contaminated than material obtained from retail butchers' shops. only 1.4% of minced meats and sausage meats contained campylobacters. most isol ... | 1985 | 4020105 |
enrichment medium for isolation of campylobacter jejuni-campylobacter coli. | a broth enrichment medium for the improvement of isolation of campylobacter jejuni-campylobacter coli from stool samples and other specimens is presented. of 1,228 samples examined in parallel, positive results were obtained from 81 by direct inoculation of selective media and from 112 after enrichment. thus, an increase of 27.7% in the isolation rate was obtained by using the enrichment medium. the same medium without antibiotics allows the preservation of isolates of c. jejuni-c. coli for at l ... | 1985 | 4026287 |
transmission of campylobacter jejuni by the housefly (musca domestica). | houseflies (musca domestica) were infected with campylobacter jejuni after being confined for 5 days in a horsfall isolator containing 25-day-old chickens known to be fecal excretors of the organism. contaminated flies, when subsequently transferred to a second unit, transmitted c. jejuni to specific-pathogen-free chickens. allowing a sample of 32 houseflies to ingest c. jejuni in a liquid suspension resulted in recovery rates of 20% from the feet and ventral surface of the body and 70% from the ... | 1985 | 4026732 |
role of litter in the transmission of campylobacter jejuni. | autoclaved or non-autoclaved used broiler litter that was experimentally contaminated with campylobacter jejuni was capable of infecting specific-pathogen-free chicks maintained in modified horsfall isolators. artificially infected chicks became fecal shedders of c. jejuni, resulting in contamination of both autoclaved and non-autoclaved used broiler litter. fecal shedding of c. jejuni by litter-reared, artificially infected chicks persisted for at least 63 days after chicks were transferred to ... | 1985 | 4026733 |
community-wide surveillance of campylobacter jejuni infection. evaluation of a laboratory-based method. | surveillance of infections due to campylobacter jejuni is needed to further define the epidemiology of this disease in the u.s. we assessed one potential method of community-wide surveillance, a laboratory-based reporting network. six microbiology laboratories that routinely culture fecal specimens for c. jejuni reported all isolates to seattle-king county health department for an 18-month period. further investigations on 476 cases reported from this broadly based network revealed that c. jejun ... | 1985 | 4028667 |
campylobacter colitis: histological immunohistochemical and ultrastructural findings. | the colonic biopsy specimens of 22 patients with colitis and positive stool cultures for campylobacter jejuni were studied in order to obtain histological and immunohistochemical criteria to differentiate campylobacter colitis from chronic inflammatory bowel disease. in addition we tried to identify campylobacter inclusions by means of immunohistochemistry and electron microscopy as evidence for invasion of the colonic mucosa. the results show that the majority of patients with campylobacter col ... | 1985 | 4029720 |
biotyping schemes for campylobacter jejuni. | | 1985 | 4031047 |
biotypes and serotypes of campylobacter jejuni and campylobacter coli strains isolated from patients, pigs and chickens in the region of rotterdam. | a comparison was made of the distribution of the bio-and serotypes of campylobacter jejuni and campylobacter coli isolated from human patients (205 isolates), intestinal contents of pigs (163 isolates) and intestinal contents and livers of chickens (147 isolates). all strains were isolated in the region of rotterdam. campylobacter jejuni biotype 1 accounted for 86%, 1% and 82% of the human, pig and chicken isolates respectively; c. coli was present in 7%, 98% and 14% respectively. serotyping was ... | 1985 | 4031520 |
serum antibodies to heat-labile enterotoxin of campylobacter jejuni. | | 1985 | 4031551 |
a point-source outbreak of campylobacteriosis associated with consumption of raw milk. | after a one-day field trip to a minnesota farm, 22 (45%) of 49 third-grade students and three (14%) of 21 adult chaperones developed campylobacteriosis. campylobacter jejuni was isolated from specimens of 13 children and one asymptomatic adult. illness was associated with the consumption of raw milk during a picnic lunch (odds ratio = 41.0, p less than .0001) and participation in hand milking of cows (odds ratio = 37.5, less than .0001). two additional findings implicated consumption of raw milk ... | 1985 | 4031557 |
experimental infection of hamsters with campylobacter jejuni. | campylobacter jejuni is a major cause of diarrhea and enterocolitis in humans and animals. a mammalian model has been developed for studying pathogenesis of the disease in hamsters by colonizing the ileum and cecum with c. jejuni via either oral intubation after purgation or direct surgical inoculation into the cecum. this colonization resulted in production of disease including diarrhea and intestinal lesions that resembles enterocolitis due to c. jejuni in humans and domestic animals. the muco ... | 1985 | 4038721 |
occurrence of plasmids and antibiotic resistance among campylobacter jejuni and campylobacter coli isolated from healthy and diarrheic animals. | serologically defined strains of campylobacter jejuni and campylobacter coli from healthy and diarrheic animals were examined for the occurrence of plasmid dna in association with the antibiotic susceptibility of the bacterial host and the health status of the animal host. of all campylobacter organisms surveyed, 53% (116 of 200) contained plasmid dna. a plasmid occurrence rate of 73.8% was obtained for c. coli from healthy pigs, contrasted by lower plasmid occurrence rates for c. coli from diar ... | 1985 | 4044793 |
hypogammaglobulinemia and recurrent campylobacter jejuni infection. | | 1985 | 4045252 |
experimental infection of gnotobiotic mice with campylobacter jejuni: colonisation of intestine and spread to lymphoid and reticulo-endothelial organs. | axenic and monoxenic c3h mice were used to develop an animal model for enteroinvasiveness and translocation of campylobacter jejuni. after oral administration of 10(7)-10(8) viable cells of c. jejuni on day 0 (d0), bacterial colonisation was followed quantitatively during 23 days by counting free luminal bacteria and tissue-associated bacteria in the duodenum, ileum and colon. the kinetics of bacterial colonisation were the same in axenic and monoxenic mice; bacteria were more numerous in distal ... | 1985 | 4045991 |
epidemiologic and laboratory investigation of an outbreak of campylobacter enteritis associated with raw milk. | an outbreak of gastroenteritis occurred in march-april 1981, in wichita, kansas, and involved more than 250 persons who drank raw milk from a single local dairy. campylobacter jejuni was recovered from 60 of 116 (52%) persons in households that had one or more ill family members. a cohort study of families that belonged to a food cooperative that purchased raw milk from the implicated dairy showed a significant association between illness and having drunk raw milk. thirty-nine of 55 (71%) person ... | 1985 | 4050775 |
comparison of methods for isolating campylobacter jejuni from raw milk. | the method of doyle and roman (appl. environ. microbiol. 43:1343-1353, 1982) was compared with that of lovett et al. (appl. environ. microbiol. 46:459-462, 1983) for the ability to recover campylobacter jejuni strains inoculated into raw milk at a concentration of less than 1 cell per g. the method of lovett et al. gave significantly greater recovery proportions. | 1985 | 4051491 |
serotyping of campylobacter jejuni from an outbreak of enteritis implicating chicken. | an outbreak of campylobacter enteritis involving 7 of 17 people over a period of 5 days followed a dinner at a restaurant. a chicken casserole dish was implicated with a food-specific attack rate of 58%. campylobacter jejuni penner serotype 18/21/29, resistant to metronidazole, was isolated from 3 of 4 symptomatic patients and from three raw fresh chicken samples closely associated with the implicated chicken. numbers of c. jejuni in the chicken ranged from 5.3 x 10(1) to 7.5 x 10(2) colony form ... | 1985 | 4056451 |
motility as an intestinal colonization factor for campylobacter jejuni. | the colonization of the intestinal tract of suckling mice by campylobacter jejuni was examined by orally challenging the mice with a wild-type strain and several nonmotile mutant strains which were isolated after treating the wild-type strain with mutagens. the wild-type strain had colonized the lower portion of the small intestine, the caecum and the colon 2 d after inoculation. two nonmotile strains, one of which (m8) had lost all the flagellar structure including the filament, the hook and th ... | 1985 | 4056739 |
chemically defined media for auxotyping of campylobacter jejuni. | a set of chemically defined media has been developed for the cultivation of campylobacter jejuni strains of human origin. a minimal medium, a complete medium and 5 different nutrient-deficient media (ndm1-ndm5) are described. some of the strains investigated required l-methionine(lacking in ndm1), l-cystine and l-cysteine (ndm2), k2hpo4 (ndm 3), kh2po4 (ndm4) and nad, thiamine and calcium pantothenate (ndm5). 57.7% of the strains investigated required l-methionine. the strains grew at ph 6.6-7.7 ... | 1985 | 4060921 |
reversible expression of flagella in campylobacter jejuni. | campylobacter jejuni 81116 and a3249 undergo a bidirectional transition between flagellated (fla+) and aflagellated (fla-) phenotypes. when measured in culture medium, the fla+----fla- transition occurred at a rate of 3.1 x 10(-3) to 5.9 x 10(-3) per cell per generation, and the fla- to fla+ transition occurred at a rate of 4.0 x 10(-7) to 8.0 x 10(-7) per cell per generation. however, passage through a rabbit intestine markedly favored the fla+ phenotype. | 1985 | 4066041 |
investigations on the role of flagella in the colonization of infant mice with campylobacter jejuni and attachment of campylobacter jejuni to human epithelial cell lines. | the biochemical and biological properties of the flagella of campylobacter jejuni have been investigated using two variants selected from a flagellate, motile clinical isolate (strain 81116): a flagellate, non-motile variant (sf-1) and an aflagellate variant (sf-2). phenotypic and biochemical analysis of the strains and amino acid analysis of the isolated flagella suggest that the variants differed from the wild-type strain only in the absence of flagella and/or motility. the aflagellate variant ... | 1985 | 4067286 |
[seasonal distribution of campylobacter jejuni and campylobacter coli (1 september 1982--31 august 1983)]. | | 1985 | 4068889 |
[bacteriological and serological evidence for the presence of campylobacter jejuni in poultry, animals and man (1982-1983)]. | | 1985 | 4070879 |
comparison of gauze swabs and membrane filters for isolation of campylobacter spp. from surface water. | the epidemiology of campylobacter jejuni indicates that waterborne transmission is important; the organism has been isolated from seawater, fresh water, and estuarine sites. membrane filtration, with and without use of an enrichment broth, has been the most common method for isolating c. jejuni from water. we evaluated two methods for isolating c. jejuni from water: membrane filtration and gauze filtration. the membrane filters evaluated included 0.22- and 0.45-micron-pore millipore filters (mil ... | 1985 | 4073894 |
inhibition of campylobacter coli and campylobacter jejuni by antibiotics used in selective growth media. | the ability of campylobacter coli and campylobacter jejuni to grow in the presence of antibiotics used in selective growth media was compared. mic data for c. coli indicated that some strains were more susceptible to the antibiotics than were the c. jejuni strains tested. a reduction of greater than 1 log cycle in the numbers of cells growing on plates containing antibiotics was considered to be a marked level of inhibition. only one of nine of the antibiotic combinations studied did not markedl ... | 1985 | 4077962 |
[campylobacter jejuni infections: epidemiology and possibility of prevention]. | | 1985 | 4079992 |
efficacy of media and methods for detecting and enumerating campylobacter jejuni in refrigerated chicken meat. | a study was undertaken to compare several enrichment and direct isolation media for their suitability to detect and enumerate five strains of campylobacter jejuni in refrigerated (5 degrees c) chicken meat. the influence of co2 on survival at 5 degrees c was also investigated. selective enrichment media evaluated included preston broth (pb), selective semisolid brucella medium (ssbm), campylobacter enrichment broth (ceb), vtp brucella-fbp broth (vtp), rosef and kapperud campylobacter enrichment ... | 1985 | 4083888 |
effect of free-radical and oxygen scavengers on photochemically generated oxygen toxicity and on the aerotolerance of campylobacter jejuni. | exposure of a nutrient agar medium to the combined action of fluorescent light and air produced toxic factors in the medium which affected the growth of campylobacter jejuni. sodium dithionite (5-10 mm), a powerful reducing agent, and catalase were effective in counteracting the injurious action of light and air. among the quenchers of singlet oxygen tested, only histidine had a beneficial effect on the recovery of c. jejuni in the photo-oxidized medium, while the addition of superoxide dismutas ... | 1985 | 4086409 |
severe campylobacter infection in children. | children suffering from campylobacter jejuni infection and who were admitted to children's hospitals in bristol and newcastle over a period of 2 years are reviewed. gastro-intestinal disease caused by c. jejuni was as common as that due to salmonella spp. three neonates suffered particularly severe infections which resembled necrotising enterocolitis. one such case is described. | 1985 | 4086864 |
[electron microscopy study of coccoid forms of campylobacter jejuni]. | | 1985 | 4089328 |
occurrence of campylobacter jejuni in diarrhoeic and non-diarrhoeic children in são paulo, brazil. | | 1985 | 4089456 |
susceptibility of campylobacter jejuni and campylobacter coli to macrolides and related compounds. | the susceptibility of 105 thermophilic campylobacters from human and swine origins to eight macrolides and related compounds was tested. erythromycin, josamycin, clindamycin, and ase 136 bs (a new erythromycin derivative) were the most active against the human strains. the swine strains were highly resistant, except to pristinamycin. the human campylobacter coli strains (except for two strains) behaved like the c. jejuni strains. | 1985 | 4091531 |
evaluation of an enzyme-linked immunosorbent assay (elisa) for the detection of campylobacter jejuni antibodies, and comparison with a complement fixation test (cft). | an enzyme-linked immunosorbent assay (elisa) was developed for the detection of total anti-campylobacter immunoglobulins in human sera. in this assay disintegrated campylobacter bacteria were used as the antigen. absorption tests including other possibly enteropathogenic bacterial species showed that the elisa system displayed a high immunological specificity for campylobacter. using this elisa it was found that in about 80% of campylobacter patients these campylobacter antibodies are produced t ... | 1985 | 4091537 |
comparison of enrichment broths for isolation of campylobacter jejuni. | growth of campylobacter jejuni was compared in enrichment broths of doyle and roman (appl. environ. microbiol. 43:1343-1353, 1982) and park et al. (c. e. park, z. k. stankiewicz, j. lovett, and j. hunt, can. j. microbiol. 27:841-842, 1981), as modified by lovett et al. (j. lovett, d. w. francis, and j. m. hunt, appl. environ. microbiol. 46:459-462, 1983). inoculated foods used were cream-pudding types, which may be cross-contaminated by improper handling, improper storage of meats prepared simul ... | 1985 | 4091559 |
isolation of campylobacter jejuni from market chickens: effect of temperature and time of incubation. | during a 4-month period, starting in june 1983, campylobacter jejuni was isolated at 42% from a total of 115 samples of market chickens. the effects of the temperature and duration of incubation on the isolation of c. jejuni were evaluated. the samples were massaged in polyethylene plastic bags, filtered through cheesecloth, centrifuged, and the sediment was plated on a selective medium. isolation of c. jejuni was increased by incubating at 42 degrees c during 48 h as opposed to 35 degrees c for ... | 1985 | 4096141 |
presence of zoonotic pathogens (yersinia spp., campylobacter jejuni, salmonella spp., and leptospira spp.) simultaneously in dogs and cats. | the purpose of this study was to determine the presence of zoonotic pathogens simultaneously in animals. the isolation of human pathogenic yersinia enterocolitica (ye), yersinia pseudotuberculosis (yp), campylobacter jejuni (cj), salmonella spp. (sal) and leptospira spp. (lep) in 318 cats and 252 dogs were performed in shimane prefecture, japan. a total of 13 isolates of yp (4 strains) and sal (9 strains) were recovered from intestine and/or mesenteric lymph nodes (mln) of 13 cats (4.1%) but not ... | 1985 | 4096152 |
aetiology of diarrhoea in adequately nourished young african children in durban, south africa. | two inter-related hospital-based studies on the causes of diarrhoea in african children who were not overtly malnourished, were performed over a period of two years in durban, south africa. the first study involved 126 inpatients selected for previously untreated diarrhoea of less than 96 h duration. on examination and culture of the stools of these children bacteria were identified in 60%, rotavirus in 20% and parasites in 4%. no pathogens were identified in 33%. the bacteria most frequently en ... | 1984 | 6084467 |
campylobacter infections in the united states. results of an 11-state surveillance. | in january 1982, 11 states (alabama, arizona, georgia, illinois, minnesota, new mexico, oregon, texas, vermont, washington, and wisconsin) began reporting monthly their isolations of campylobacter to the centers for disease control in atlanta. the information reported included the species of campylobacter organisms, the week of the report, the site from which the organism was isolated, and the age and sex of the infected person. a total of 3,966 isolates were reported in 1982, of which 3,900 wer ... | 1984 | 6087756 |
prevalence of enteric pathogens in the feces of healthy beef calves. | fecal specimens from 136 healthy beef calves (1 day to 12 weeks of age) were examined for the presence of infectious agents known to cause enteric disease in calves. the calves were selected from 22 herds in which all calves were free of clinically apparent enteric disease. salmonella sp, enterotoxigenic escherichia coli, cryptosporidium, and coronavirus were not detected in any of the calves. three calves were infected with rotavirus and 1 calf was infected with yersinia enterocolitica. campylo ... | 1984 | 6089621 |
micro-organisms in gastroenteritis. | we present bacteriological and virological findings together with salient clinical features from a prospective study of 447 children aged under 2 years admitted to hospital with infectious gastroenteritis. putative pathogenic micro-organisms were identified in the stools of 75% of these children. eight identifiably distinct groups of viruses, found on electron microscopy and tissue culture were present in 67% of patients--rotavirus was detected most frequently. pathogenic bacteria (salmonellas, ... | 1984 | 6091568 |
three-year prospective study of intestinal pathogens in madrid, spain. | during the period july 1980 through june 1983, in a general hospital in madrid, the following organisms were detected from 6,970 patients with gastroenteritis: 710 salmonella spp. 506 campylobacter jejuni, 379 shigella spp., 12 yersinia enterocolitica, 1,466 rotavirus, 134 giardia lamblia, and 4 entamoeba histolytica. chloramphenicol showed good activity against most tested strains of salmonella spp., shigella spp., and c. jejuni. the incidence of salmonella spp. and shigella spp. was very marke ... | 1984 | 6092418 |
campylobacter jejuni enteritis transmitted from cat to man. | | 1980 | 6103127 |
campylobacter jejuni enteritis transmitted from cat to man. | | 1980 | 6104005 |