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possible role of leukotrienes in gastritis associated with campylobacter pylori.this study was done to evaluate the role of leukotrienes (lts) in gastritis associated with campylobacter pylori. biopsy specimens of gastric mucosa were obtained endoscopically from 18 patients with nonulcer dyspepsia for bacteriological and histological examination and extraction of lts. there was correlation between the ltb4 level in the mucosa and the degree of gastritis evaluated histologically. the level was higher when infiltration of neutrophils in the gastric mucosa was more extensive. ...19902170499
comparison of anti-helicobacter (campylobacter) pylori igg antibodies between different areas of belgium.we have collected sera from 4053 patients of different parts of belgium. sera were randomly selected whatever the kind of pathology. anti-helicobacter (campylobacter) pylori igg were determined with an elisa technique using whole formalized bacteria. the results suggest that the mean antibody titres differ between various areas, with an overall higher prevalence in the north-western part of the country.19902173316
genotypic variation in 'campylobacter upsaliensis' from blood and faeces of patients in different countries.chromosomal dna from 62 strains of 'campylobacter upsaliensis' from blood and faecal specimens of patients in australia, belgium, south africa and england, and seven dog isolates from england and sweden were examined. dna base compositions of 21 strains were 35 +/- 1 mol% g + c. electrophoresis of haeiii dna digests gave patterns comprising about 20 well resolved bands of 2 to 10 kb, and visual comparisons revealed considerable genomic variation with 26 different digest patterns identifiable. di ...19902178140
comparison of different tests for helicobacter pylori. 19902178404
empiric antimicrobial therapy of domestically acquired acute diarrhea in urban adults.from june 1985 to september 1987, 202 adults were enrolled in a randomized, double-blinded study comparing ciprofloxacin (500 mg) with sulfamethoxazole and trimethoprim (160 mg/800 mg) or placebo for adults with acute diarrhea. all patients were treated on the day of presentation and received medication on a twice-daily schedule (every 12 hours) for 5 days. bacterial isolates from these patients included 35 campylobacter, 18 shigella, and 15 salmonella. treatment at the time of presentation with ...19902178582
helicobacter pylori: controversies and an approach to management.helicobacter pylori (formerly, campylobacter pylori) is a gram-negative, spiral-shaped bacterium with a strong affinity for gastric-type epithelium. convincing evidence indicates that h. pylori plays an etiologic role in the development of chronic, nonspecific gastritis, and it may play an important role in the pathogenesis of duodenal ulcer disease. an etiologic role for this organism in chronic gastric ulceration, nonulcer dyspepsia, and gastric carcinoma is not established. whereas the diagno ...19902179647
public health and research perspectives on the microbial contamination of foods.scientific advances in methodology and epidemiology have resulted in a renewed awareness of foodborne disease, and increased contact among nations of the world has stimulated rapid global distribution of foods as well as foodborne pathogens. new food vehicles are being identified for old, familiar pathogens, and new pathogens are being discovered. current research in food microbiology has spurred development of rapid and specific methods to identify these pathogens and to assess their virulence. ...19902180884
rapid urease tests for campylobacter pylori. 19902180988
biotypes, serogroups and antibiotic susceptibility of campylobacter jejuni and campylobacter coli in chile.phenotypic markers were studied in 105 strains of thermophilic campylobacters isolated from human beings, animals and drinking water in santiago, chile. the strains were identified as campylobacter jejuni (n = 49) and campylobacter coli (n = 56). biotypes i and ii (lior schema) accounted for 96% c. jejuni isolates, the other 4% being biotype iv but the two biotypes of c. coli were about equally represented. a total of 28 serogroups (lior's heat-labile antigens) were identified. lior 13, 9, 79, 2 ...19902181024
helicobacter pylori and the pathogenesis of gastroduodenal inflammation.helicobacter pylori is a newly discovered gram-negative bacterium that lives in the human stomach and duodenum. infection with this organism is strongly associated with type b antral gastritis and with peptic ulcer disease. recent evidence from human volunteer studies, therapeutic trials with antimicrobial agents, and experiments with animal models indicates that h. pylori plays an etiologic role in the pathogenesis of type b gastritis. gastric metaplasia is observed in virtually all patients wi ...19902181029
chronic gastritis and ulcer risk. 19902181619
identification of campylobacter pylori in gastric biopsy smears.the use of gastric biopsy imprint smears to diagnose campylobacter pylori was compared with the use of tissue sections and cultures. multiple gastric biopsies were taken from the mucosa of 42 patients during endoscopy. imprint smears were prepared from the samples used to make tissue sections; other samples were used for microbiologic culture. there was a good concordance (93%) between the morphologic diagnosis of c pylori in the air-dried, giemsa-stained smears and the tissue sections; the cyto ...19902181802
evaluation of a selective enrichment technique for the isolation of campylobacter pylori.to cultivate campylobacter pylori from contaminated biopsy specimens, brucella broth was supplemented with 10% fetal calf serum, 1% vitox, 1000 units/ml polymyxin b sulfate, 10 micrograms/ml vancomycin, and 2 micrograms/ml amphotericin b. pseudomonas aeruginosa, candida albicans, and enterococcus fecalis were cocultivated with c. pylori. all four strains of c. pylori were recoverable at 24 h. when 21 c. pylori strains were studied in pure culture, 86% grew in the selective enrichment medium. in ...19902182378
molecular studies on the mechanism of tetracycline resistance mediated by tet(o).the mechanism of resistance to tetracycline in escherichia coli mediated by the campylobacter jejuni-derived resistance determinant tet(o) was investigated. the cloned tet(o) protein had no detectable effect on the intracellular accumulation of tetracycline. the presence of tet(o) markedly diminished the inhibitory effect of tetracycline on protein synthesis both in vivo and in vitro. ribosomes prepared from tetracycline-resistant and susceptible e. coli cells bound almost identical amounts of r ...19902183711
campylobacter pylori: a new pathogen.there is now considerable evidence suggesting that c. pylori is a human pathogen. the strong association between c. pylori and gastroduodenal disease is well documented. a number of hypotheses have been suggested for the pathogenic mechanisms of c. pylori-induced gastroduodenal disease, including the production of cytotoxins and the mechanical disruption of the epithelium. at the present time, treatment with a combination of antimicrobial agents eradicates the infection in approximately 50% of c ...19902183821
drug-induced changes of plasma gastrin concentration.there is a significant inverse relationship between intragastric acidity and plasma gastrin concentration. all generally available gastric acid antisecretory drugs induce a release of gastrin into the circulation. the more potent the gastric antisecretory dosage regimen or drug, the greater the rise of plasma gastrin concentration. the drug-induced rise of plasma gastrin concentration is of no direct clinical concern, although it may be partly responsible for the phenomenon of tolerance to h2-bl ...19902184125
campylobacter pylori and its role in peptic ulcer disease.in almost all patients with genuine nondrug-induced duodenal or gastric ulcer there is evidence of gastric campylobacter pylori colonization and concomitant inflammation. c. pylori is only demonstrable in the duodenal cap when there is "gastric mucus metaplasia." suppression or eradication of c. pylori with antibiotic therapy may accelerate duodenal ulcer healing. the relapse rate of duodenal ulcer is less after temporary clearing or permanent eradication of c. pylori. as eradication of c. pylor ...19902184128
[campylobacter pylori-helicobacter pylori. a long development]. 19902184426
campylobacter (helicobacter) pylori. is peptic disease a bacterial infection? 19902184791
microbiological agents associated with childhood diarrhoea in the dry zone of sri lanka.between march and july, 1987, faecal specimens from 371 diarrhoeal children reporting to four hospitals in the district of kurunegala, sri lanka and from 121 controls with other diseases were investigated for enteric pathogens. all specimens were tested for shigella spp, salmonella spp and enteropathogenic escherichia coli (epec). in addition, parasitic pathogens, heat labile enterotoxigenic escherichia coli (etec lt), campylobacter and rotavirus were sought among subsamples of the cases and con ...19902187225
factors responsible for the introduction and spread of campylobacter jejuni infection in commercial poultry production.campylobacter jejuni and related thermophilic campylobacters were not found in a hatchery or in chicks aged less than seven days. however, an increasing proportion of chicks aged two weeks and older shed campylobacters in their droppings. it was shown that a likely source of c jejuni for young chicks was the environment in the immediate vicinity of the rearing houses, and that infection could readily be introduced on the footwear and clothing of farm staff. thermophilic campylobacters were found ...19902188414
purification and characterization of urease from helicobacter pylori.urease was purified 112-fold to homogeneity from the microaerophilic human gastric bacterium, helicobacter pylori. the urease isolation procedure included a water extraction step, size exclusion chromatography, and anion exchange chromatography. the purified enzyme exhibited a km of 0.3 +/- 0.1 mm and a vmax of 1,100 +/- 200 mumols of urea hydrolyzed/min/mg of protein at 22 degrees c in 31 mm tris-hcl, ph 8.0. the isoelectric point was 5.99 +/- 0.03. molecular mass estimated for the native enzym ...19902188975
enterocecocolitis associated with intraepithelial campylobacter-like bacteria in rabbits (oryctolagus cuniculus).we examined 28 suckling, weanling, and young adult rabbits with lethargy, inappetence, and mucinous, semifluid feces. sixteen of the rabbits had intestinal lesions. in eight of these rabbits, the primary changes were multifocal to diffuse epithelial proliferation and accumulation of lymphocytes, macrophages, or both in the lamina propria of the small intestine, cecum, and sacculated colon. in two of these rabbits, the accumulation of macrophages in the lamina propria was extensive. the other eig ...19902189255
application of a new phagetyping scheme to campylobacters isolated during outbreaks.a new scheme for phagetyping campylobacters has been evaluated using strains isolated from five outbreaks. the phagetyping results have been compared with the results of penner serotyping, lior serotyping and preston biotyping. phagetyping recognized the causative strains in all of the incidents and also differentiated these strains from animal and environmental strains isolated during these investigations. in some outbreaks phagetyping proved to be more discriminatory than serotyping or biotypi ...19902189742
[evaluation of norfloxacin in the pediatric field. pediatric study group for norfloxacin].norfloxacin (nflx, am-715), a new quinolone antibiotic agent, was evaluated clinically and bacteriologically for its efficacy and safety in pediatrics by a study group organized with pediatricians from all over the country. a summary of the results of the evaluation is as follows. 1. incidence of nflx-resistant strains (mic over 12.5 micrograms/ml) isolated from children with various infections was 1.6% (8/512). one resistant strain was observed among 45 isolates of staphylococcus aureus, and no ...19902190014
gastroduodenal ulcers: causes, diagnosis, prevention, and treatment.the precise diagnosis and duration of peptic ulcer disease can now be made endoscopically. numerous effective medical therapies are available to treat these disorders. while the vast majority of patients respond to medical therapy with complete healing of ulcers within 4 to 6 weeks, a small percentage require longer and probably more intense treatment. a total assessment of the patient's lifestyle is important in order to achieve the best therapeutic response. cigarette smoking is a potent negat ...19902190751
identification of campylobacter cinaedi isolated from blood and feces of children and adult females.five campylobacter-like organisms isolated from blood and feces were identified by numerical analysis of gel electrophoretic protein profiles and immunotyping as campylobacter cinaedi. two of these strains were isolated from adult females; the remaining three strains were isolated from children, two of whom were girls. c. cinaedi has hitherto been isolated only from rectal swabs and blood of homosexual and bisexual males with gastrointestinal symptoms. the results presented extend our knowledge ...19902191002
to the problem of campylobacter jejuni detectability in water.in a series of model experiments two isolation procedures for the detection of water-borne campylobacter jejuni were compared: a standard culture in thioglycolate broth enriched with 7% defibrinated sheep blood and supplement c and a modified membrane filtration method in which the filter (porosity 0.45 microns) plated on campylobacter agar surface was removed after the first 24 hours of incubation and the plate further incubated for 48 hours. the recovery rates by the thioglycolate broth method ...19902191035
[current views on the etiology and pathogenesis of peptic ulcer]. 19902191515
[infections caused by bacteria of the campylobacter genus]. 19902192208
[campylobacter pylori and its role in the etiopathogenesis of upper digestive tract diseases]. 19902192401
cardiovascular and bacteremic manifestations of campylobacter fetus infection: case report and review.a case of bacteremia due to campylobacter fetus subspecies fetus with concomitant pleuropericarditis in a previously healthy patient is presented. the organism is ubiquitous, but most commonly causes infection in patients with chronic underlying illnesses. the pathogenesis of human infection has not been definitively elucidated. bacteremia is the most common clinical manifestation of this infection, although cases of thrombophlebitis, mycotic aneurysm, endocarditis, and pericarditis have also be ...19902193344
[helicobacter pylori and gastric pathology]. 19902193600
[surgical treatment of infectious aneurysm of the popliteal artery. description of a case caused by campylobacter jejuni and a review of the literature].the authors describe a case of mycotic aneurysm of the popliteal artery secondary to campylobacter jejuni-derived infectious endocarditis treated by excision and in situ femoro tibial venous bypass, and take the opportunity to review 14 literature cases of infectious aneurysm of popliteal artery. this diagnosis is most frequently evoked by the development in an infectious setting of a throbbing inflammatory mass in the popliteal fossa. management is aimed at controlling the infection and insurin ...19902193936
[evaluation of effectiveness of rokitamycin dry syrup in acute enteritis in pediatrics. a comparative study on rokitamycin and fosfomycin dry syrups].because campylobacter jejuni is most frequently identified as a causative organism of bacterial enteritis in pediatrics, a study was done to evaluate the clinical efficacy against campylobacter enteritis and the safety of a macrolide antibiotic, rokitamycin (rkm). in case of acute enteritis, rkm was used in a form of dry syrup at a dose level of approximately 30 mg (in potency)/kg body weight and its efficacy and safety were compared to those of fosfomycin (fom) dry syrup which is currently in u ...19902194055
aetiology of diarrhoea in pre-term neonates at kenyatta national hospital nursery, nairobi, kenya.the aetiology of two outbreaks of diarrhoea in pre-term neonates (march-august; september, 1987), at kenyatta national hospital was studied. the first outbreak involved 98 neonates and enteropathogenic e. coli of different serotypes were the most commonly isolated agents (54%), with serotype 086a:k61 dominating. these were followed by salmonella (16%) also of different groups, and then rotavirus (6%). two campylobacter and two shigella were isolated from four individual neonates. mixed infection ...19902194778
[does helicobacter pylori have a direct proteolytic effect in ulcerative disease?].helicobacter pylori (h.p.), has been shown, experimentally, to exert a proteolytic activity against mucous fractions. aim of this study was to assess the prevalence of h.p. in peptic ulcer and to analyze its possible influence on gastric mucus components, on peptic activity in gastric juice and the possible action on peptic secretion. 223 patients undergoing upper gastrointestinal endoscopy were analyzed for the presence of h.p. in the mucosa: 99 duodenal ulcer patients (d.u.), 58 gastric ulcer ...19902194877
gastritis in the elderly.the elderly have an increased prevalence of gastritis and the complications associated with it. the two major classes of acute and chronic gastritis, type a and b, are discussed as they relate to clinical findings, histologic features, and pathogenesis. an age-related increase in the frequency of helicobacter pylori (formerly campylobacter pylori) infection in the elderly parallels the known age-related increase in the prevalence of gastritis. the close association between gastritis in the elder ...19902194946
morphology and immunoperoxidase studies of intestinal adenomatosis in the blue fox, alopex lagopus.in a blue fox farm about 300 of 400 weanling pups were affected with diarrhoea and prolapse of the rectum. about 40 pups died in an emaciated state after a few days of disease. eight pups were submitted for examination. pathological findings were restricted to the caecum, proximal colon and rectum, with occasional extension to the ileum. affected areas revealed a thick and rigid intestinal wall, a narrow lumen and a thickened, wrinkled and sometimes ulcerated mucosa. histologically, the mucosa s ...19902195067
vacation diarrhea. how should it be managed?traveler's diarrhea in north america is most often a self-limited disease that requires little medical intervention unless the course is prolonged or especially severe. diagnosis is almost always made by a stool examination or culture. the most common causative organisms are campylobacter jejuni and giardia lamblia. enterotoxigenic escherichia coli is a less frequent cause. patients should be advised regarding the need for fluid replacement. antibiotic therapy should be considered only in rare c ...19902195482
helicobacter pylori (formerly campylobacter pyloridis/pylori) 1986-1989: a review. 19902196282
a simple method to detect helicobacter pylori in gastric specimens. 19902196955
bismuth therapy in gastrointestinal diseases.bismuth therapy has shown efficacy against two major gastrointestinal disorders: peptic ulcer disease and diarrhea. in peptic ulcer disease it is as effective as the h2-receptor antagonists, costs considerably less, and offers a lower rate of relapse. when helicobacter pylori is implicated, bismuth acts as an antimicrobial agent, suppressing the organism but not eliminating it. in recent studies, bismuth compounds have been used with conventional antibiotics, producing elimination of the organis ...19902199292
acute gastroenteritis due to double infection with enteropathogenic escherichia coli or salmonella and another bacterial pathogen.two enteric bacterial pathogens were concomitantly isolated from the feces of 18 infants less than 6 months of age admitted to the assaf harofeh medical center over a 7-year period. in all but two patients stool cultures grew enteropathogenic escherichia coli, six of serogroup 0119. the other organisms cultured were shigella, salmonella, campylobacter jejuni and aeromonas hydrophila. the usual clinical presentation was diarrhea, dehydration and vomiting of acute onset, and low grade fever. patie ...19902199408
campylobacter pylori. a new era in gastroduodenal disorders? 19902199425
isolation, characterization, and host-cell-binding properties of a cytotoxin from campylobacter jejuni.a 68,000-molecular-weight protein was isolated by polyacrylamide gel electrophoresis from the organism-free filtrate of a fully virulent clinical strain of campylobacter jejuni. the eluted protein was heat labile, was inactivated at either ph 3.0 or 9.0, was sensitive to trypsin, and was lethal for fertile chicken eggs. it also had toxic effects on chicken embryo fibroblast, chinese hamster ovary (cho), and intestinal 407 (int407) cells. a monoclonal antibody (cetpmab4) raised to this eluted tox ...19902199497
evaluation of the indoxyl acetate hydrolysis test for the differentiation of campylobacters.indoxyl acetate hydrolysis is a rapid, inexpensive differential test which can be performed easily to help identify campylobacter. a total of 571 campylobacter cultures, including atypical variants, representing 10 species was tested.19902199514
immunocytochemical detection of helicobacter pylori in formalin fixed tissue biopsy specimens. 19902199535
survival of helicobacter pylori in water and saline. 19902199542
search for campylobacter species in the public water supply of a large urban community.a survey of the water distribution network of a large urban community was performed during a one year period. in addition to standard indicator bacteria, campylobacter species were looked for after filtration of 500 ml water samples by enrichment of the original filter followed by subculture on a skirrow's medium as well as a direct filtration procedure on a non selective agar. all steps were performed at 37 degrees c. specimens collected at the springs (206), treatment stations (206) and at dif ...19902200424
identification of campylobacter pylori by gastric brush cytology. 19902201508
helicobacter pylori: bridging the credibility gap.in summary, therefore, there are interesting associations between h pylori, duodenal ulcer, and non-ulcer dyspepsia. in type b gastritis there may be enough evidence to suggest a causal role. the relation between gastritis and upper gastrointestinal symptomatology, however, remains contentious. the relation between h pylori and acid secretion may be more intimate than was previously thought. 'pylorites' must temper their enthusiasm and provide hard data; 'schwartzians' must broaden their horizon ...19902201586
helicobacter (= campylobacter) pylori in africa.epigastric pain is a common complaint throughout africa, mostly in the form of non-ulcer dyspepsia. it has recently been suggested that helicobacter (= campylobacter) pylori, a bacterium that colonizes the gastric mucosa and causes type b gastritis, may be the cause of this epigastric pain. this paper reports the endoscopic, histological and bacteriological findings in 57 patients presenting with epigastric pain to the university of maiduguri teaching hospital during one year, together with a re ...19902202107
similarities in the microfloras of root canals and deep periodontal pockets.although not universally accepted, retrospective histological, roentgenological and microbiological studies have indicated that cross-infection can occur between infected pulps and deep periodontal pockets. this review provides examples of similarities in the microfloras of these adjacent oral sites, supporting the idea that infection spreads from one site to the other. the organisms most often involved are probably bacteroides, fusobacteria, eubacteria, spirochetes, wolinellas, selenomonas, cam ...19902202588
comparison of some enrichment broths and growth media for the isolation of thermophilic campylobacters from surface water samples.three different enrichment broths and two selective growth media were compared for isolating thermophilic campylobacters by combined membrane filtration and enrichment techniques from surface waters of different physical, chemical and bacteriological characteristics. fifty-two strains of campylobacters were isolated from total of 1668 cultures. the various broth/medium combinations did not affect the dominance of c. jejuni over c. coli (total 49 c. jejuni and three c. coli). the most efficient c ...19902202704
correlation between human and animal bio-serogroups of campylobacter isolates in nigeria.campylobacter enteritis is endemic in nigeria as in most other developing countries of the world. this paper examines the common biotypes and serogroups of campylobacter in human and animal isolates. the observed correlation suggests a possible animal to human route of infection in nigeria. implications on preventive and control measures are discussed.19902202840
campylobacter pylori in israel: prospective study of prevalence and epidemiology.antral biopsy samples were taken from 147 patients undergoing gastroscopy. campylobacter pylori was cultivated from 100 of these patients. c. pylori was isolated from 76% of the specimens showing any degree of histologic gastritis, but from only 11% of specimens with completely normal histology. a questionnaire was completed on all patients and included demographic, epidemiologic and clinical information. sephardic origin, smoking, and a bad taste in the mouth were more prevalent in the campylob ...19902205596
gastritis. 19902205940
the radiologic and endoscopic investigation and etiologic classification of gastritis in children.in 11 children the endoscopic criteria for gastritis were correlated with radiologic and pathological findings. an etiologic classification is proposed. the diagnoses included gastric involvement by crohn's disease (five children), eosinophilic gastroenteritis (one), diffuse varioliform gastritis (two), erosive gastritis associated with campylobacter pylori infection (two) and idiopathic erosive gastritis (one). eight of the nine double-contrast barium studies and only one of the three single-co ...19902207775
an outbreak of campylobacter enteritis associated with a community water supply.in june 1987, an outbreak of acute enteritis occurred over a period of 2 weeks in a small rural community where the potable water supply is unfiltrated and unchlorinated. campylobacter jejuni was isolated from 6 patients. a case-control study performed in a local fiberglass shop where workers drink a lot of water, showed an association between the occurrence of enteritis and the consumption of 10 or more 8-ounce glasses of water per day (odds ratio = 6, p = 0.04). the attack rate of enteritis wa ...19902207948
identification of campylobacter pylori by endoscopic brush cytology.to investigate the value of papanicolaou-stained endoscopic brush samples in the diagnosis of campylobacter pylori infection of the upper gastrointestinal tract, 138 brush and biopsy samples from the esophagus, stomach, and duodenum, taken concomitantly, were reviewed retrospectively. in 35 cases, campylobacter-like organisms (clos) were found in both cytology and biopsy samples. in 15 cases, clos were seen in biopsy material only, and in 8 cases, clos were found in cytology material only. clos ...19902209347
[occurrence and significance of different campylobacter types in domestic animals in hungary].experiences, including results of original experimental work on campylobacter fetus, c. jejuni and c. coli induced diseases of cattle, sheep, dogs, rabbits poultry and men in hungary are reviewed. out of 31 cases of abortion in cows 29 (93.5%) were causes by c. fetus subsp. venerealis and only one case each (3.2%) by c. fetus subsp. fetus and c. jejuni, respectively. out of the 29 strains of c. fetus subsp. venerealis, 26 belonged to serogroup 01 (a) and only 3 to serogroup 02 (b). campylobacter ...19902209454
infection with bacterial enteropathogens in swedish travellers to south-east asia--a prospective study.infection with potential bacterial enteropathogens was studied prospectively in 94 swedish travellers. three faecal samples were collected, regardless of diarrhoeal symptoms, after each of three 1-week stays in singapore, hong kong and japan. the specimens were analyzed for salmonella, shigella, yersinia, campylobacter and different enterotoxin-producing bacteria. a potential enteropathogen was identified in 30% (28/94) of the participants, i.e. in 26% of the healthy and in 39% of the travellers ...19902209737
development of a human stomach explant organ culture system to study the pathogenesis of helicobacter pylori.these studies were undertaken to define conditions under which helicobacter (formerly campylobacter) pylori and viable human gastric mucosa could coexist in tissue culture with the ultimate goal of developing an in vitro experimental model which could be used to study interactions between h. pylori and gastric epithelium. antral gastric biopsies obtained at upper endoscopy were placed in culture in either cmrl-1066 or keratinocyte growth media and incubated at 37 degrees c in either an oxygen-en ...19902210096
dna analysis of intracellular campylobacter-like organisms associated with the porcine proliferative enteropathies: novel organism proposed.intracellular campylobacter-like organisms are a consistent feature of the porcine proliferative enteropathies. the relationship between these organisms and known campylobacter sp. previously associated with the disease was studied using restriction enzyme analysis and dna-dna blot hybridization techniques. bglii restriction enzyme fragment patterns of dna of the campylobacter-like organisms were fundamentally different from those of c. mucosalis, c. hyointestnalis, c. jejuni, and c. coli. crude ...19902210329
identification of catalase negative/weak campylobacter jejuni from human blood and faecal cultures by numerical analysis of electrophoretic protein patterns.twenty-eight isolates of catalase-negative/weak (cnw) thermophilic campylobacters from human blood and faecal cultures were characterized by one-dimensional (1-d) high-resolution sds-page of cellular proteins. a further 11 campylobacter strains were included for reference purposes. partial protein patterns were used as the basis for numerical analysis, which showed that all of the hippurate-positive strains had a high similarity to c. jejuni. two subclusters were formed within c. jejuni correspo ...19902210345
adjuvant antibiotic therapy in duodenal ulcers treated with colloidal bismuth subcitrate.persistence of helicobacter pylori after duodenal ulcer healing is associated with high rates of ulcer relapse. we compared colloidal bismuth subcitrate alone with cbs combined with one of four antibiotic regimens in the treatment of duodenal ulcers. endoscopy and antral biopsies were performed before treatment and four weeks afterwards. biopsy specimens were examined for histological evidence of gastritis and by gram stain and culture for h pylori infection. altogether 141 patients were allocat ...19902210469
structural and functional analysis of two campylobacter jejuni flagellin genes.the purpose of this study was to characterize the flagellin gene of campylobacter jejuni and to study the structure of this protein and the regulation of its synthesis. a part of the flagellin gene of c. jejuni strain 81116 was recently cloned by us. this dna fragment was used as a probe to isolate the other homologous flagellin sequences from genomic libraries. the flagellin nucleotide sequence was determined from overlapping clones. two copies of the flagellin gene were identified: genes fla a ...19902211662
the susceptibility of campylobacter pylori to antiulcer agents and antibiotics.the antibacterial activities of antiulcer agents and antibiotics against campylobacter pylori were studied. the mic90 values of three kinds of antibiotics--macrolides, beta-lactams, and metronidazole--were 0.05-0.78, 0.39-1.56, and 12.5 micrograms/ml, respectively. they were more active than antiulcer agents such as h2-blockers and cetraxate with mic90 values of greater than or equal to 1,600 and greater than 1,600 micrograms/ml, respectively. especially, clarithromycin, a new derivative of eryt ...19902212539
evidence for gastric mucosal cell invasion by c. pylori: an ultrastructural study.it is now generally accepted that campylobacter pylori is closely associated with peptic ulcer disease and chronic type b gastritis. whether c. pylori is the direct etiologic cause of either or both of these illnesses remains unclear. possible pathophysiologic effects of c. pylori are still a matter of debate and conjecture. utilizing a small group of patients with gastric ulcers and chronic gastritis, we examined the ultrastructural relationship between c. pylori and gastric cells. forty-eight ...19902212556
campylobacter pylori interactions with gastric cell tissue culture.many investigators have reported that gastric mucosal biopsies of patients with chronic gastritis and peptic ulcer disease show the presence of campylobacter pylori in a large majority of cases. histologic examinations of such tissues indicate a close approximation of c. pylori with gastric surface epithelial cells. a recent report has described both adherence and cell invasion of gastric cells by c. pylori. using a transmission electron microscope, we have examined the interaction between c. py ...19902212557
[comparison of three methods for detecting campylobacter pylori and measurement of human antibody titers against the whole organisms].in order to detect campylobacter pylori in the gastric mucosa, three different methods as 1) culture of the organisms, 2) immunostaining by monoclonal antibody against the cells, and 3) urease test were compared. in the disease group, positive % of each methods was 64, 75 and 74. however, positive % common to the three methods was only 48. the corresponding rate of culture and urease tests, and that of culture and staining methods was 81% and 60%, respectively. therefore, it was concluded that u ...19902212754
organ hypertrophy and responses of colon microbial populations of growing swine to high dietary protein.thirty-two castrated male crossbred growing pigs (average initial wt 26.9 kg) were used to determine the effect of a high level of dietary protein (37%) compared with a normal level of protein (15%) on enterobacteria and campylobacter sp. inhabitation in the large intestine and on visceral organ hypertrophy and the interrelationships between these two factors. pigs were kept in pairs (eight pens of two pigs/diet) and fed their respective diets and libitum. eight pigs (two pens of two pigs fed ea ...19902213253
preliminary study on the microbiology of campylobacter pyloridis and gastric histopathology.biopsy samples were taken endoscopically from the antral-mucosa of 693 patients with peptic ulcer and chronic gastritis presenting dyspepsia symptoms. campylobacter pyloridis cultures were positive in 59 of 98 (60.2%) cases and histopathologically the organisms were found in 411 of 693 cases (59.3%). pathologically, campylobacter pyloridis was positive in 273 out of 300 patients with chronic superficial gastritis (91.0%), in 102 of 249 patients with chronic atrophic gastritis (40.9%), in 36 out ...19902214500
[campylobacter pylori in patients with peptic ulcer and chronic gastritis: immunologic response]. 19902214616
the use of sensitivity discs in the identification of campylobacter species.filter paper discs were impregnated with a solution containing 20 mg of triphenyltetrazolium chloride per millilitre, and used in the typing of catalase-positive campylobacter species. also used were filter paper discs impregnated with cephalothin at 30 micrograms/ml, 60 micrograms/ml and 3 mg/ml and nalidixic acid at the same concentrations, as well as commercially available discs containing 30 micrograms of, respectively, cephalothin and nalidixic acid. results obtained proved the technique to ...19902216345
[campylobacter pylori and its role in the pathogenesis of selected diseases of the stomach and duodenum]. 19902216966
salmonella-reactive synovial fluid t-cell clones in a patient with post-infectious salmonella arthritis.from a patient with reactive arthritis following salmonella typhimurium enteritis, synovial fluid t-lymphocytes were cloned and expanded in vitro. seven out of 74 clones showed a marked proliferative response to antigens of heat-killed salmonella typhimurium with autologous t-cell-depleted peripheral blood mononuclear cells as antigen-presenting cells. the salmonella-reactive clones were of the cd4+ phenotype, antigen-induced proliferation could be inhibited by a monoclonal antibody to hla class ...19902218431
[etiology of diarrheal diseases in immunocompetent and hiv-positive patients].in 1986 and 1987 the stool samples of 206 patients of the medical outpatient clinic basel were examined prospectively for bacteria, protozoa and worms. clinical data of the patients were recorded by questionnaire. the patient group comprised 63 immunocompetent patients as well as 23 hiv-infected patients, all with symptoms of acute enteritis. the control group consisted of 120 healthy persons. pathogenic organisms were found in the stools of 17.5% of the immunocompetent patients with enteritis. ...19902218447
[the histological diagnosis of chronic gastritis. a german proposal for an integrated nomenclature].the gastrointestinal pathology study group of the german pathological society has drawn up a proposal for a unifying nomenclature for the graduation and classification of gastritis. the severity of chronic gastritis is divided into 4 grades (minimal, light, moderate and severe) and the activity of the process is characterized by 3 grades (grade 1, 2 and 3 activity). in addition, the presence or absence of helicobacter pylori (formerly campylobacter pylori), the extent and type of intestinal meta ...19902218460
[campylobacter pylori and treatment of duodenal ulcer].the authors subjected to endoscopic examination 90 patients with duodenal ulcers. from each patient they collected a bioptic specimen of the gastric antrum and duodenal bulbus for microbiological and histological examination. they found that for detection of c. pylori the microscopic examination was most useful. the presence of c. pylori in the gastric antrum was detected in 87% of the subjects and in the duodenal bulb only in 48%. the difference is significant at the 0.01 level. to 43 patients ...19902219764
hyperplastic gastric polyps associated with persistent helicobacter pylori infection and active gastritis.we report two cases of patients with 3-yr histories of upper gastrointestinal symptoms, hyperplastic gastric polyps, and active chronic gastritis. biopsies retrospectively stained with giemsa revealed the persistent presence of helicobacter pylori (hp) in gastric biopsies of both patients throughout the 3 yr. after treatment with amoxicillin and bismuth subsalicylate, both became asymptomatic, one demonstrating disappearance and recurrence of the gastric polyps in conjunction with the hp. these ...19902220735
the prevalence of helicobacter pylori in nonulcer dyspepsia. importance of stratification according to age.helicobacter pylori (formerly campylobacter pylori) is causally related to active antral gastritis and is highly associated with duodenal and gastric ulcers. however, the relationship of h pylori to nonulcer dyspepsia is less clear. we determined the presence of h pylori in unselected patients who were undergoing upper gastrointestinal tract endoscopy, and we found a prevalence of 37% in 110 patients with nonulcer dyspepsia that was similar to previous data. patients with nonulcer dyspepsia who ...19902222090
[combination of glandular and foveolar hyperplasia of the gastric mucosa--a rare mixed type].in a 28 year-old man with epigastric pain endoscopy detected giant gastric folds and multiple superficial erosions. histologically a mixed form of glandular and foveolar hyper plasia of the gastric mucosa was suggested (ming's type iii). basal and pentagastrin stimulated secretion volume and acid output were moderately elevated, hypersecretion of protein was not found. serum levels of calcium and gastrin were normal, also after secretin stimulation. the mucosa of the corpus was extensively infes ...19902222791
revised nomenclature of campylobacter laridis, enterobacter intermedium, and "flavobacterium branchiophila". 19902223613
[advances in the research of pathogenicity of campylobacter pylori and epidemiology of its infection]. 19902225011
comparative in vitro activity of cefpirome and cefepime, two new cephalosporins.in in vitro tests the broad-spectrum cephalosporins cefpirome and cefepime were highly active against enterobacteriaceae, although often less so against strains resistant to amoxicillin-clavulanate and ticarcillin-clavulanate, and against most strains of acinetobacter spp. and aeromonas hydrophila. they were also active against pseudomonas aeruginosa, although strains with non-plasmid mediated beta-lactam resistance were sometimes less sensitive. other pseudomonas spp. varied in their sensitivit ...19902226498
natural history of congestive gastropathy in cirrhosis. the liver study group of v. cervello hospital.in a prospective study of the natural history of congestive gastropathy, 212 consecutive cirrhotic patients (75 treated with sclerotherapy) were included. mean follow-up was 46 months. mild gastropathy (mosaiclike pattern) was found in 110 patients and severe gastropathy (granular mucosa with cherry spots) was found in 20. prevalence of helicobacter pylori, formerly campylobacter pylori, was 50% in patients without, 43% in those with mild, and 28% in those with severe gastropathy. congestive gas ...19902227271
evaluation of cytotoxic activity in fecal filtrates from patients with campylobacter jejuni or campylobacter coli enteritis.we sought to determine the prevalence of cytotoxic activity in fecal filtrates from persons with c. jejuni or c. coli enteritis. stool specimens were collected from 20 persons with c. jejuni or c. coli enteritis, 20 persons with acute diarrheal illnesses of other causes, and 9 healthy, asymptomatic persons. fecal filtrates were then incubated with chinese hamster ovary (cho) or hela cells. the fecal filtrate from 1 of the 20 (5%) persons with campylobacter enteritis was cytotoxic for hela cells ...19902227365
chloramphenicol resistance in campylobacter coli: nucleotide sequence, expression, and cloning vector construction.a chloramphenicol-resistance determinant (cmr), originally cloned from campylobacter coli plasmid pnr9589 in japan, was isolated and the nucleotide sequence determined, which contained an open reading frame of 621 bp. the gene product was identified as cm acetyltransferase (cat), which had a putative amino acid sequence that showed 43% to 57% identity with other cat proteins of both gram+ and gram- origin. although expression of the cat gene was constitutive in both c. coli and escherichia coli, ...19902227449
ranitidine in children with peptic ulcer and patients with pancreatic cystic fibrosis.this article describes experience of the use of ranitidine in children with peptic ulcer and patients with pancreatic cystic fibrosis. ranitidine proved to be efficacious and well tolerated, the percentage of healing being 89.4%. ranitidine was also used in subjects with gastritis from campylobacter pylori, obtaining rapid regression of subjective symptoms. administration of ranitidine to cystic fibrosis patients improved the efficacy of the pancreatic extract, with consequent enhancement of dig ...19902228343
surface array protein of campylobacter fetus. cloning and gene structure. 19902229082
gastrointestinal side-effects of octreotide during long-term treatment of acromegaly.gastrointestinal side-effects of prolonged therapy (greater than 2 yr) with the long-acting somatostatin analog octreotide were studied in 10 acromegalic patients. after 2 yr of therapy, 6 of 10 patients had newly developed gallstones, complicated by cholangitis and jaundice in 1. serum vitamin b-12 concentrations declined in all 10 patients [from 380 +/- 32 to 172 +/- 21 pmol/l (mean +/- se); p = 0.023] and became abnormally low in 4. gastric biopsy specimens, obtained during gastroscopy (9 pat ...19902229321
evaluation of the indoxyl acetate hydrolysis test for rapid differentiation of campylobacter, helicobacter, and wolinella species.a total of 410 well-defined campylobacter, helicobacter, and wolinella strains, comprising 26 named species, subspecies, and defined groups, were tested for indoxyl acetate hydrolysis by a disk method by using disks prepared at the centers for disease control, atlanta, ga. all c. coli (43 strains), c. cryaerophila (34 strains), c. fennelliae (5 strains), c. fennelliae-campylobacter-like organism 3 (2 strains), c. jejuni (66 strains), c. jejuni subsp. doylei (3 strains), hippurate-negative c. jej ...19902229360
campylobacter-like organisms are uncommon pathogens in patients infected with the human immunodeficiency virus.over a 25-month period, we prospectively evaluated 36 patients with symptomatic human immunodeficiency virus disease (including 27 with unexplained chronic diarrhea) by flexible sigmoidoscopy for the presence of campylobacter-like organisms. no campylobacter-like organisms were isolated. campylobacter-like organisms appear to be an uncommon cause of idiopathic chronic diarrhea in symptomatic human immunodeficiency virus disease.19902229366
prevalence of campylobacter infections in animals and children in haryana, india.to study the prevalence of infections with campylobacter spp in haryana, india, a stool sample was collected using a rectal swab from 30 buffaloes, 62 cattle, 95 pigs and 94 children and was bacteriologically cultured. the subjects were either apparently healthy or had diarrhoea. the organisms were isolated in a medium containing reducing agents and antibiotics, and culture plates were incubated in a candle jar at 42 degrees c. 63% of all thermophilic campylobacter were cultured from rectal swab ...19902229989
[the serotype distribution of campylobacter jejuni strains among gastroenteritis in hospitals over 7 year period in tokyo].campylobacter jejuni strains isolated from gastroenteritis at 4 general hospitals of tokyo metropolitan during the period from 1981 to 1987 were serotype according to the slide agglutination test (tck system) developed by the tokyo metropolitan research laboratory of public health. two thousand four hundred seventy-nine strains isolated from sporadic cases among infants and children, 1,962 (78.5%) were typed by 33 typing sera numbered tck 1 through tck 33 and leaving 537 strains (21.5%) untypabl ...19902230367
[serology and culture diagnosis of campylobacter jejuni infections].campylobacter jejuni is known today as one of the most common pathogens in acute infectious enteritis. in 77 patients serological testing by complement fixation as well as stool cultures were performed. a campylobacter jejuni infection was identified in 53% by culture and in 64% by serology, only in 17% the diagnosis was made with both methods. we conclude, that because of these data stool culture and serological testing should be used simultaneously. the complement fixation technique shows an i ...19902233597
a newly recognized fastidious gram-negative pathogen as a cause of fever and bacteremia.we identified a motile, curved, gram-negative bacillus as the cause of persistent fever and bacteremia in two patients with symptomatic human immunodeficiency virus infection. the same organism was subsequently recovered from a bone marrow-transplant recipient with septicemia and from two immunocompetent persons with week-long febrile illnesses. all the patients recovered after antimicrobial therapy.19902233947
[campylobacter pylori infection. results of an investigation of 130 patients].after recalling the main data on diagnosis, pathogenesis and treatment of cp infection, the results of a study on 130 patients are presented.19902234480
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