| gamma globulin therapy for chronic mononucleosis syndrome. | antibodies against epstein-barr virus, associated with antibody dependent cytotoxic cell activity, were found to be present in diminished titer in 20 of 22 patients tested with chronic mononucleosis syndrome (cms). gamma globulin was shown to improve symptoms in 53% of the patients treated, compared with 32% of placebo injections. 89.5% of 57 patients treated with a gamma globulin treatment program remained in the treatment program because of relief of symptoms, and only four patients dropped ou ... | 1986 | 2435296 |
| immune response to intermediate filament-associated, epstein-barr virus-induced early antigen. | a murine monoclonal antibody (mab) (15td3) was found to recognize a unique filamentous structure in epstein-barr virus (ebv)-producing lymphoblastoid cell lines. by immunofluorescent morphology, in comparison with a control mab to vimentin, the 15td3 filamentous structure was judged to be associated with intermediate filaments of the cytoskeleton. expression of the 15td3 antigen and vimentin was induced simultaneously in some ebv genome-positive cell lines either by ebv superinfection or by 12-0 ... | 1987 | 2435803 |
| nuclear dna-binding proteins determined by the epstein-barr virus-related simian lymphotropic herpesviruses h. gorilla, h. pan, h. pongo and h. papio. | nuclear dna-binding proteins were extracted from lymphoblastoid cell lines transformed with epstein-barr virus (ebv) or with the related lymphotropic herpesviruses of gorilla (herpesvirus gorilla), chimpanzee (h. pan), baboon (h. papio) or orang-utan (h. pongo). they were immunoblotted with the sera of all four simian species in comparison with ebv antibody-positive human sera. eight nuclear proteins were identified, and were designated gona-1 and gona-2 for h. gorilla-determined nuclear antigen ... | 1987 | 2438377 |
| analytical studies of the binding parameters describing the interaction of hla-dr epitopes with a specific monomorphic monoclonal antibody. | in this paper we present an analytical study of the binding parameters related to the interactions of the hla-dr-specific monoclonal antibody (l243) and its reacting epitope as expressed on the cell surface of seven epstein-barr virus-transformed b cell lines. scatchard, sips and langmuir equations were used to plot and analyse the data obtained from each reaction. a single affinity constant (k) value was derived at low and high concentrations of free antibody for each antibody-cell interaction ... | 1987 | 2440953 |
| a study of the ability of namalwa interferon to induce sister-chromatid exchanges in vitro. | heparinized cord blood from a newborn child and peripheral venous blood from three other individuals have been cultured in vitro to investigate whether the frequency of sister-chromatid exchange (sce) can be affected by the presence of human namalwa lymphoblastoid interferon (namalwa ifn). the results demonstrated that both purified and crude namalwa ifn failed to show higher yields of sce than normal controls (p greater than 0.05). no namalwa ifn-dependent variation was found in the frequencies ... | 1987 | 2443847 |
| gp140, the ebv/c3d receptor (cr2) of human b lymphocytes, is involved in cell-free phosphorylation of p120, a nuclear ribonucleoprotein. | gp140, the eb/c3d receptor (ebv/c3dr; cr2), is a membrane site involved in human b cell regulation. cross-linking of this receptor on the cell surface by its specific ligands led to the enhancement of b cell proliferation in synergy with t cell factors. in vitro activation of human peripheral b lymphocytes by cross-linking membrane immunoglobulins with anti-mu antibody induced ebv/c3dr phosphorylation. these studies were pursued by analyzing cell-free phosphorylation of ebv/c3dr isolated from ra ... | 1987 | 2446881 |
| new differentiation antigens associated with the growth and maturation of b lymphocytes. | monoclonal antibodies (mab) nda3 and nda4 were generated by hyperimmunizing mice with an alloreactive human t cell clone and fusing the splenocytes with the ns1 myeloma. immunofluorescence studies indicated that these mab react with activated, but not with resting t and b lymphocytes or with other types of cells. immunoprecipitation studies with 125-i-labeled extracts from t and b cell lines demonstrated that the m.w. of the antigen recognized by mab nda3 is 36,000 and that of nda4 is 46,000. st ... | 1988 | 2447175 |
| in situ hybridization to detect epstein-barr virus dna in oral tissues of hiv-infected patients. | thirty biopsies of oral mucosal lesions and normal oral mucosa were obtained from 26 hiv-seropositive individuals and studied for virus infections with epstein-barr virus-specific dna probes (ebv). in situ dna hybridization was carried out on frozen and formalin-fixed, paraffin-embedded tissues. specifically bound biotinylated virus probes were detected with the streptavidin-gold-silver technique and visualized by standard and interference reflection microscopy. in 9/30 biopsies, ebv dna was cle ... | 1987 | 2447697 |
| the killing of tumour cell targets coupled to tuberculin (ppd) by human and murine ppd-reactive t helper clones. i. ppd specificity of killing. | this paper reports on the characteristics of killing by a human and a murine tuberculin (ppd)-specific t helper clone of targets to which ppd was attached via the lectin concanavalin a (con a). the killing was specific for ppd from m. tuberculosis; and targets coupled to con a alone or to ppd from m. paratuberculosis were not killed. target cells carrying con a-ppd were more effectively lysed than ppd-pulsed cells. this form of lymphocyte killing, though highly significant, was inefficient. maxi ... | 1988 | 2451275 |
| the epstein-barr virus glycoprotein gp110, a molecular link between hla dr4, hla dr1, and rheumatoid arthritis. | | 1988 | 2452474 |
| functional polymorphism of the hla-dr beta iii chain. | several clusters of class ii hla genes contribute to variation in human antigen-presenting capacity. in the hla-dr cluster, most of the variation is due to the highly polymorphic dr beta i gene. recent work by others has shown some nucleotide and implied amino acid sequence variation in dr beta iii chains, but this variation is not known to be functionally significant. we show here that two proliferating human t-cell clones define three allelic variants of dr beta iii (assignment to dr beta iii ... | 1988 | 2452815 |
| glycolipid reanchoring of t-lymphocyte surface antigen cd8 using the 3' end sequence of decay-accelerating factor's mrna. | decay-accelerating factor (daf) is one of a family of cell-associated proteins that undergo posttranslational modifications in which glycolipid anchoring structures are substituted for membrane-spanning sequences. the signals that direct the covalent substitution reaction in these proteins are unknown. human daf was expressed in chinese hamster ovary (cho) cells and murine bw lymphocytes. in both cases, the xenogeneic daf in transfectants incorporated a glycolipid anchor. a chimeric cd8-daf cdna ... | 1988 | 2453063 |
| monoclonal antibodies against the androgen receptor: recognition of human and other mammalian androgen receptors. | monoclonal antibodies against the androgen receptor (ar) will provide useful probes for elucidating both the structure and function of this important regulatory protein. recently, human autoimmune anti-ar sera have been described. the purpose of the current work was to immortalize lymphocytes from the blood of patients with high titer anti-ar antibodies and to produce monoclonal antibodies against the receptor in vitro. human serum samples (10 microliters) were incubated in high ionic strength b ... | 1988 | 2454813 |
| immunological abnormalities in insulin receptors on cultured ebv-transformed lymphocytes from insulin-resistant patient with leprechaunism. | defects in insulin-receptor structure can impair insulin-receptor function. we have previously identified qualitative abnormalities in insulin binding to insulin receptors from an insulin-resistant patient (lep/ark-1). the defects in insulin binding are probably caused by a defect in receptor structure. in this study, we used immunological probes to investigate the structural defect(s) responsible for the abnormal function. several anti-receptor antibodies were impaired in their abilities to bin ... | 1988 | 2454859 |
| epstein-barr virus-transformed b cells bearing idiotypes of anti-dna autoantibodies. | epstein-barr virus (ebv)-transformed b cells obtained from healthy subjects had the same idiotypes of anti-dna autoantibodies on their surface as those obtained from patients suffering from systemic lupus erythematosus. these clones secreted anti-single-stranded or anti-double-stranded dna antibodies. among them, some produced anti-dna idiotype-positive antibodies but failed to bind dna. this was confirmed by a competitive inhibition radioimmunoassay. it was then considered whether or not the ex ... | 1988 | 2455731 |
| in vitro production of human antibodies against bladder cancer: characterization and diagnostic implications. | | 1987 | 2457663 |
| myelin basic protein-specific cd4+ cytolytic t-lymphocyte clones isolated from multiple sclerosis patients. | in the present report we describe the isolation and characterization of stable, long-term, human t-lymphocyte clones specific for myelin basic protein (mbp) from multiple sclerosis (ms) patients. isolation of these clones appeared possible only by seeding peripheral blood mononuclear cells into a limiting dilution microculture system containing mbp, autologous irradiated cells and interleukin-2 (il-2), thereby minimizing effects of putative suppressor cell populations. all clones obtained were o ... | 1988 | 2458331 |
| cell cycle control of a burkitt lymphoma cell line: responsiveness to growth signals engaging the c3d/ebv receptor. | cr2, the receptor for the c3d fragment of the third complement component and for epstein-barr virus (ebv) has been shown, on mouse b cells, to be involved in the control of b-cell proliferation by acting as a receptor for macrophage-derived growth factors. we examined whether the growth of a burkitt lymphoma cell line, raji, could be influenced by ligands of human cr2. in serum-free culture, purified human c3d, as well as three monoclonal antibodies to distinct epitopes on human cr2, were capabl ... | 1988 | 2461345 |
| alternative splicing of hla-dqb transcripts and secretion of hla-dq beta-chain proteins: allelic polymorphism in splicing and polyadenylylation sites. | hla class ii antigens are highly polymorphic cell-surface proteins involved in initiation and regulation of the immune response. allelic sequence variation primarily affects the structure of the first external domains of alpha and beta component chains. here we provide evidence for other types of allelic polymorphism for the genes encoding these chains. sequences of two cdna clones corresponding to hla-dqb mrnas from an hla-homozygous cell line exhibit both alternative splicing and read-through ... | 1989 | 2464826 |
| [epstein-barr virus infection and congestive cardiomyopathy (serologic and immunomorphologic aspects)]. | in serums of patients with congestive cardiomyopathy (ccm), patients with ischemic heart disease (ihd) and those of healthy subjects, presence of antibodies to capsid and early antigens of epstein-barr's virus (ebv) and to various structural components of myocardium was determined in order to study seroepidemiologic relations between the infection and ccm as well as immunopathogenic reactions against myocardium in this disease. seroepidemiologic relation between ebv and ccm consisted in signific ... | 1988 | 2466468 |
| growth inhibition of epstein-barr virus-transformed b cells by anti-hla-dr antibody l243: possible relationship to l243-induced down-regulation of cd23 antigen expression. | among five anti-hla class ii monoclonal antibodies (mab's) tested, the anti-hla-dr mab l243 selectively inhibited the in vitro proliferation of epstein-barr virus-transformed lymphoblastoid cell lines (lcl). saturating amounts of l243 mab induced 50% suppression of lcl growth as revealed by measuring [3h]thymidine incorporation or counting cell numbers. preincubation for 20 hr at 37 degrees c in the presence of l243 mab dramatically reduced the expression of certain b cell-specific antigens on l ... | 1989 | 2467753 |
| studies of sublines selected for loss of hla expression from an ebv-transformed lymphoblastoid cell line. changes in sensitivity to cytotoxic t cells activated by allostimulation and natural killer cells activated by ifn or il-2. | murine tumor studies have suggested that variations in mhc class i expression may influence the behavior of tumor cells in vivo. corresponding human tumor studies remain at the descriptive level but they show differences in mhc ag expression between and even within tumors. our purpose was to explore possible functional relationships between class i expression and sensitivity to different forms of cell-mediated lysis in a potentially immunogenic system: ebv-immortalized b cells. their cytotoxic s ... | 1989 | 2468716 |
| probing the human b-cell repertoire with ebv: polyreactive antibodies and cd5+ b lymphocytes. | | 1989 | 2469441 |
| identification of a human epithelial cell surface protein sharing an epitope with the c3d/epstein-barr virus receptor molecule of b lymphocytes. | this work examines the basis for our earlier observation that certain monoclonal antibodies (mabs) specific for the b-cell-associated c3d/epstein-barr virus (ebv) receptor molecule cd21 also react with the surface of some epithelial cells. of 9 proven anti-cd21 mabs now examined on frozen sections of human nasopharynx, tonsil and ecto-cervix, only 3 (hb5, anti-b2, ab1) showed staining of stratified epithelium; 2 of these (hb5, anti-b2) also reacted with the surface of epithelial cells freshly di ... | 1989 | 2469656 |
| human monoclonal antibodies against cytokeratin 18 generated from patients with gastric cancer. | by co-culturing regional lymph node b-cells and hat-sensitive mutant cells obtained from rpmi-1788 cells, no less than 20,000 epstein-barr (eb)-transformed colonies were obtained from 32 patients with gastric cancer. from b-cell cultures generating antibodies reactive with gastric cancer tissues as well as cultured gastric cancer cells, two eb-transformed cell clones termed c418-59 and c1218-39 were isolated. both of them produced human igm-class antibodies, termed mab418-59 and mab1218-39, resp ... | 1989 | 2470715 |
| 5-azacytidine up regulates the expression of epstein-barr virus nuclear antigen 2 (ebna-2) through ebna-6 and latent membrane protein in the burkitt's lymphoma line rael. | nonproductive infection of b lymphocytes by epstein-barr virus (ebv) is associated with a highly restricted expression of viral genes. in growth-transformed lymphoblastoid cell lines, the products of these genes include a complex of at least six ebv nuclear antigens (ebnas) (ebna-1 through ebna-6) and one membrane protein (latent membrane protein [lmp]). ebv-carrying burkitt's lymphoma (bl) biopsies and derived cell lines that have retained a representative phenotype (group i bl lines) express o ... | 1989 | 2470924 |
| hairy leukoplakia: epstein-barr virus receptors on oral keratinocyte plasma membranes. | hairy leukoplakia (hl) is an oral white lesion associated with, and probably caused by, the epstein-barr virus (ebv) among persons who are seropositive for infection with human immunodeficiency virus. a unique feature of hl is its localization to the lateral portion of the tongue. to determine site differences for ebv receptors according to epithelial phenotype, these receptors were mapped in oral mucosa with the use of monoclonal antibodies hb5 and b2(specific for the complement fraction 3d/ebv ... | 1989 | 2471127 |
| a monoclonal antibody to lfa-3, the cd2 ligand, specifically immobilizes major histocompatibility complex proteins. | t cells are activated when the antigen-specific t cell receptor recognizes antigen in association with major histocompatibility complex (mhc) proteins. the t cell surface protein cd2 (t11, lfa-2, the t erythrocyte receptor) and its target or stimulator cell ligand, lymphocyte function-associated antigen-3 (lfa-3), are also involved in t cell adhesion and activation. the molecular mechanisms by which the cd2/lfa-3 interaction affects t cell adhesion and activation are unclear. the cd2/lfa-3 inter ... | 1989 | 2471647 |
| [diagnostic significance of determining antibodies to antigens of the epstein-barr virus in infectious mononucleosis]. | infectious mononucleosis may be differentiated from clinically similar diseases by the detection of classes m and a antibodies to epstein-barr virus capsid antigen. the authors emphasize the high specificity of this test vs. the detection of the paul-bunnell-davidson heterophilic antibodies. | 1989 | 2473271 |
| ebna-1: a virally induced nuclear antigen of primate lymphocytes and its expression in drosophila cells. | ebna-1 is a nuclear antigen of lymphocytes infected by epstein-barr virus and whose size polymorphism correlates only with the strain of infecting virus and the length of the glycine-alanine copolymer encoded by the third internal repeat of the viral genome. the major antigenic determinant(s) also appear to reside in this region. we have been able to obtain efficient expression of this nuclear antigen in cultured drosophila cells transfected with a cosmid carrying the ebna-1 coding region, indic ... | 1988 | 2474313 |
| human monoclonal antibodies with a protective activity against tetanus toxin. | the antibodies and their protective activity in response to tetanus toxoid in man were studied by producing human antitetanus monoclonal antibodies after transformation of peripheral blood lymphocytes with epstein barr virus. two human monoclonal igg that reacted with the heavy chain of the toxin were obtained. one of them binds the cooh-terminal moiety and the other the nh2-terminal moiety. only the nh2-terminal specific monoclonal antibody neutralized toxin in mouse, but in doses approximately ... | 1989 | 2475149 |
| 'fetal-type' b and t lymphocytes in rheumatoid arthritis and primary sjögren's syndrome. | b lymphocytes expressing cd5 (cd5+b cells) and t lymphocytes using the gamma and delta chains to form their antigen receptor (gamma delta +t cells) are major populations in developing fetuses, but become relatively minor in normal adults. however, both subsets are expanded in the peripheral blood of more than 50% of patients with rheumatoid arthritis and primary sjögren's syndrome. we have examined the surface phenotype of these subsets using flow cytometry and have studied the frequency of igm- ... | 1989 | 2476142 |
| hla-dr molecules on all cells are electrophoretically unique. | previous sds-page studies of autologous clonal all and normal b cell lines indicated that hla-dr molecules on leukemic cells have an extra beta chain band. we now show that these results are not an artifact of cell lines, ebv transformation, or cell growth in culture. leukemic cells from four all patients were surface labeled with 125i and their hla-dr molecules compared with those on autologous normal peripheral blood b cells. the electrophoretic patterns of hla-dr molecules on these in vivo ce ... | 1989 | 2477648 |
| induced expression from the moloney murine leukemia virus long terminal repeat during differentiation of human myeloid cells is mediated through its transcriptional enhancer. | transcription from the moloney murine leukemia virus (mo-mulv) long terminal repeat (ltr) is inhibited in murine stem cells and induced during maturation of these cells. we have investigated whether alterations in the activity of this viral regulatory element also occur during differentiation of human myeloid leukemia cells. the mo-mulv ltr and the simian virus 40 (sv40) early promoter were introduced into hl-60 promyelocytes on epstein-barr virus-derived chloramphenicol acetyltransferase expres ... | 1989 | 2477690 |
| human b cell lines secreting igm antibody specific for myelin basic protein. | in this study we describe for the first time the production of stable human b cell lines and clones that secrete igm antibody specific for human myelin basic protein. the technique based on limiting dilutions of epstein-barr virus-transformed peripheral b cells from patients with multiple sclerosis, precluded the need for preselecting or stimulating antigen-specific b cells. most of the cell lines were stable for at least 6 months in continuous culture and produced 5-12 micrograms/ml antibody af ... | 1989 | 2478580 |
| characterization of human cd5 (leu-1, okt1)+ b lymphocytes and the antibodies they produce. | | 1989 | 2479500 |
| ebv gene expression in an npc-related tumour. | a nasopharyngeal carcinoma tumour (designated c15) propagated in nude mice has been used to generate a large cdna library that we have analysed for epstein-barr virus (ebv) gene expression. no gross alterations exist in viral dna from c15 relative to other human isolates and the large deletion present in the b95-8 'prototype' viral strain established in marmoset cells is not found; c15 contains no linear virion dna. in the cdna library, of the six ebv nuclear antigens (ebnas) expressed in latent ... | 1989 | 2479554 |
| mapping of the epstein-barr virus and c3dg binding sites to a common domain on complement receptor type 2. | complement receptor type 2 (cr2;cd21), a member of the superfamily of proteins containing short consensus repeats (scrs), is the b cell receptor for both the gp350/220 envelope protein of epstein-barr virus (ebv), and for the c3dg protein of complement. by analysis of cr2 deletion mutants and chimeras formed with cr1 (cd35) we determined that of the 15 scrs in cr2, the nh2-terminal two scrs are necessary and sufficient to bind both gp350/220 and c3dg with affinities equivalent to those of the wi ... | 1989 | 2479703 |
| long-term culture and fine specificity of human cytotoxic t-lymphocyte clones reactive with human immunodeficiency virus type 1. | the definition of human immunodeficiency virus type 1 (hiv-1) immunogenic epitopes is central to the rational design of aids vaccine strategies. in this study, we have generated seven hiv-1 reverse transcriptase-specific cytotoxic t-lymphocyte (ctl) clones from the peripheral blood of two seropositive subjects. epitopes recognized by these ctl clones were identified by using target cells infected with recombinant hiv-1-vaccinia virus vectors expressing truncated reverse transcriptase proteins an ... | 1989 | 2480604 |
| construction of an ebv-derived shuttle vector for studying the influence of transcription on mutagenesis. | we have constructed an ebv-derived shuttle vector, pf1-ebv, which replicates in human cells as an extrachromosomal element. the structural sequences of the gene encoding the bacterial xanthine-guanine-phosphoribosyltransferase (gpt) were fused to the promoter and presumptive control region of the mouse metallothionein i (mt-i) gene. human 293 cells transformed with the recombinant plasmid synthesized gpt mrna and the expression of the gene was inducible by zinc. the gpt gene offers a convenient ... | 1989 | 2480810 |
| effects of interferons on human b-cell differentiation in vitro. | | 1987 | 2484953 |
| [anti-rana antibodies and rheumatoid arthritis]. | anti rana antibodies were determined by the method of indirect immunofluorescence in 51 patients with the classic resp. definitive form of rheumatoid arthritis (ara) and in 19 healthy persons. anti rana antibodies were found in 92% of the patients with rheumatoid arthritis and in 16% of the control group. the reactive nuclear antigen is present in human b lymphocyte tissue cell cultures transformed by the epstein-barr virus--raji cells. the anti rana antibody test is very suitable for use in the ... | 1989 | 2491398 |
| transformation of human cells by oncogenic viruses supports permissiveness for parvovirus h-1 propagation. | parvovirus h-1 has been shown to suppress spontaneous and chemically or virally induced tumorigenesis in hamsters. in human cell culture systems propagation of h-1 is restricted to transformed cells, which are killed by h-1 infection, in contrast to normal diploid cells, which are nonpermissive for h-1. by analyzing the permissiveness of a variety of human cells for h-1, it was determined that the majority of tested transformed or immortalized cells which were permissive for h-1 contained the dn ... | 1989 | 2495371 |
| assessment of human allospecific il-2-secreting helper t lymphocytes in limiting dilution cultures using restimulation and split well analysis. | a limiting dilution (ld) culture was established which allows the detection of allospecific interleukin-2 (il-2)-secreting helper t lymphocyte precursors (htl-p) among human peripheral blood mononuclear cells (pbmnc). htl-p stimulated with allogeneic epstein-barr virus-transformed b lymphoblastoid cell lines (ebv-lcl) in the presence of exogenous recombinant il-2 (r-il-2) clonally developed into il-2 secreting effector cells when restimulated against the original stimulating alloantigen. split w ... | 1989 | 2526838 |
| a survey of epstein-barr virus dna in lymphoid tissue. frequent detection in hodgkin's disease. | a total of 151 unselected malignant and nonmalignant lymphoid tissue samples were surveyed by southern blotting for the presence of epstein-barr virus (ebv) dna. eight of 28 hodgkin's disease (hd) samples (29%) had detectable ebv dna. both nodular sclerosis and mixed cellularity histologic results were positive. the tumor type with the next highest frequency, 8%, was diffuse large cell lymphoma. the presence of ebv dna in some hd biopsies suggests that ebv may be a factor in the pathogenesis of ... | 1989 | 2535912 |
| a phenotypic study of cells from burkitt lymphoma and ebv-b-lymphoblastoid lines and their relationship to cells in normal lymphoid tissues. | cells of 7 ebv-b-lcl, 10 burkitt lines and 13 ebv-b-lcl/burkitt line hybrids have been phenotyped for antigens of the major b cell clusters and for some other antigens. high levels of cd23 and cd39 and low levels of cd38 (t10) were characteristic of ebv-b-lcl; the converse was true for burkitt lines. in hybrids the ebv-lcl phenotype was dominant. the phenotype of burkitt-line cells correlated strongly with that of germinal centre b cells in tonsil sections, but differed markedly from that of mar ... | 1989 | 2536003 |
| epstein-barr virus-infected b cells persist in the circulation of acyclovir-treated virus carriers. | in this study, infectious epstein-barr virus (ebv) shedding in the oropharynx and numbers of virus-infected b cells in the blood have been monitored in long-term virus carriers receiving acyclovir (acv) therapy for herpes zoster. eleven patients on oral acv were followed prospectively before, during and for 2 weeks after treatment. as expected, the low levels of ebv shedding in these virus carriers (measured as cord-blood lymphocyte transforming activity in throat washings) were eliminated durin ... | 1989 | 2536009 |
| clonal and molecular characteristics of the human ige-committed b cell subset. | we have followed the pathway of the ige-committed b lymphocyte from fresh, unstimulated peripheral blood, through ebv activation, transformation, and eventual cloning. using cell sorting in conjunction with limiting dilution culture systems, we found that: (a) cells that are selected in the cell sorter and secrete ige in culture are sigm+/sigd+. they secrete all three isotypes after ebv activation and continue to do so stably in culture; (b) individual ige+ cells in culture coproduce igm, igd, a ... | 1989 | 2536066 |
| aids-associated non-hodgkin's lymphoma in san francisco. | the characteristics of acquired immunodeficiency syndrome-associated non-hodgkin's lymphoma in 84 patients diagnosed and treated at san francisco general hospital are presented herein. while the majority were high-grade b-cell lymphomas, one cutaneous t-cell and one peripheral t-cell lymphoma were observed. in addition, three other tumors were suspicious for t-cell lymphoma. sixty-seven percent of patients had stage iv disease, often at unusual sites. epstein-barr virus dna sequences were identi ... | 1989 | 2536124 |
| psychiatric diagnoses in patients who have chronic fatigue syndrome. | patients with persistent fatigue are often suspected of having psychiatric illnesses, particularly depression. the authors used the diagnostic interview schedule to assess the lifetime prevalence of psychiatric disorders in 28 patients who met centers for disease control case definition criteria for chronic fatigue syndrome. compared with studies of the general population and studies of chronically medically ill patients who received the same structured interview, the rates of psychiatric illnes ... | 1989 | 2536690 |
| electric field-induced cell fusion and human monoclonal antibodies. | a persistent problem in the generation of antigen-specific human monoclonal antibodies is the rarity of appropriate b cells in human blood or splenic tissues. in order to immortalize the rare antigen-specific cells that are available, an electric field-induced cell fusion technique has been shown to markedly increase the fusion efficiency in comparison to polyethylene glycol-induced cell fusion using epstein-barr virus (ebv) or pokeweed mitogen activated b cells. fusion efficiency of 10(-3)-10(- ... | 1989 | 2536773 |
| the epstein-barr virus and chronic fatigue syndrome. | | 1989 | 2536851 |
| [epstein-barr virus infections. new pathogenic and clinical aspects]. | the epstein-barr virus (ebv) is among the most widespread of human viruses. it causes several different diseases, such as acute infectious mononucleosis (im), chronic active ebv-infection (cebv), the x-linked lymphoproliferative syndrome (xlp), polyclonal and oligoclonal lymphomae in connection with immunologic disorders, as well as african burkitt's lymphoma and nasopharyngeal carcinoma. pathogenesis, clinical features and diagnosis are discussed. in this connection, special tests on the latenc ... | 1989 | 2536874 |
| detection of epstein-barr viral genomes in reed-sternberg cells of hodgkin's disease. | we used slot blot hybridization, southern blot hybridization, and in situ hybridization to investigate the presence of epstein-barr virus (ebv) genomes in biopsy tissues from patients with hodgkin's disease. slot blot hybridization performed on dna of tissue specimens from 16 patients revealed that biopsy tissue from 3 (19 percent) contained ebv dna. southern blot hybridization with a dna probe containing the 500-base-pair tandem repeated sequences located at the termini of the ebv genome confir ... | 1989 | 2536894 |
| the multiple membrane-spanning segments of the bnlf-1 oncogene from epstein-barr virus are required for transformation. | the bnlf-1 gene from epstein-barr virus (ebv) induces anchorage-independent and tumorigenic growth in rodent cell lines. the bnlf-1 protein (also termed lmp) is a membrane protein, and its predicted amino acid sequence indicates that the protein has six membrane-spanning segments in addition to a short amino-terminal (approximately 25 amino acids) and a long carboxyl-terminal (approximately 200 amino acids) cytoplasmic domain. to identify the regions of the protein that are essential for its tra ... | 1989 | 2536919 |
| transformation and plasmacytoid differentiation of ebv-infected human b lymphoblasts by ras oncogenes. | the biological effects of ras oncogene activation in b cells were studied by using amphotropic retroviral vectors to introduce h- or n-ras oncogenes into human b lymphoblasts immortalized by epstein-barr virus. expression of both h- and n-ras oncogenes led to malignant transformation of these cells, as shown by clonogenicity in semisolid media and tumorigenicity in immunodeficient mice. in addition, terminal differentiation into plasma cells was detectable as specific changes in morphology, immu ... | 1989 | 2536954 |
| phenotypic analysis of human myeloma cell lines. | multiple myeloma (mm) is a b-cell malignancy characterized by the accumulation, primarily in bone marrow, of a clone of plasma cells. the nature of the stem cells feeding the tumoral compartment is still unknown. to investigate this special point, we have studied the phenotypes of nine well-known human myeloma cell lines (hmcls) and compared them with those of normal lymphoblastoid cell lines (lcls). twenty-four clusters of differentiation involved in b lymphopoiesis were investigated using a pa ... | 1989 | 2537114 |
| spontaneous epstein-barr virus transformed b-cell line sharing the identical immunoglobulin gene rearrangement with acute myeloid leukemia. | mononuclear cells from a 44-year-old patient with acute myeloid leukemia (aml) gave rise to a spontaneous permanent cell line cultured in suspension. the cell line was shown to be positive for epstein-barr virus nuclear antigen (ebna). as expected, its composite phenotype was of b-cell type with b-cell antigens (cd 20, cd 21) and with monoclonal surface igm of kappa type, but without detectable igm secretion. surprisingly, identical monoclonal rearrangements of the immunoglobulin heavy chain (jh ... | 1989 | 2537117 |
| modulation of lymphocyte proliferation and immunoglobulin production by transferrin-gallium. | gallium resembles iron with respect to transferrin (tf) binding and cellular uptake via tf receptors. we have previously shown that transferrin-gallium (tf-ga) complexes interfere with the cellular incorporation of iron and inhibit the proliferation of hl60 cells. since mitogen-stimulated peripheral blood lymphocytes express tf receptors, we examined the effect of tf-ga on lymphocyte proliferation and on immunoglobulin synthesis by b-lymphocytes. tf-ga inhibited phytohemagglutinin, pokeweed mito ... | 1989 | 2537143 |
| class i cross-restricted t cells reveal low responder allele due to processing of viral antigen. | cytotoxic t lymphocytes (ctl) recognize protein antigens which have been processed by the target cell and then presented in association with the relevant class i molecule of the major histocompatibility complex (mhc). short synthetic peptides, which are able to associate directly with target cells, may substitute for these processed fragments in stimulating antigen-specific ctl responses. using this approach, a dominant hla-a2-restricted epitope has previously been mapped to residues 58-68 of in ... | 1989 | 2537466 |
| [chronic virus infections. mechanisms and clinical significance]. | numerous viruses are known to cause chronic infections in humans. such infections are clinically important because of their association with a variety of disorders. the article reviews the mechanisms and clinical importance of such viruses, using herpes simplex virus, epstein-barr virus and human immunodeficiency virus as examples. | 1989 | 2537543 |
| complete characterization of the gene coding for the epstein-barr virus major membrane antigen gp 220/340 and selective expression of a secreted form of gp 220. | the gene which encodes the epstein-barr gp 220/340 was inserted into a eukaryotic expression vector. a cdna clone corresponding to the mature mrna coding for gp 220 was isolated from an epstein-barr virus cdna library and inserted in the same expression vector, enabling us to identify the precise location of the intron within the gp 220/340 coding sequence. the recombinant plasmids direct the expression of membrane proteins detected by immunofluorescence experiments using an anti-gp 220/340 mono ... | 1989 | 2537632 |
| epstein-barr virus infections following okt3 treatment. | | 1989 | 2538016 |
| properties of glucocorticoid receptors in epstein-barr virus-transformed lymphocytes from patients with familial cortisol resistance. | in a previous report of two patients with familial glucocorticoid resistance due to reduced numbers of glucocorticoid receptors (gr), we have shown decreased numbers of gr in peripheral mononuclear cells and cultured fibroblasts but normal affinity of gr in both patients. in this study, peripheral lymphocytes from these patients, one patient's son and daughter, and normal subjects were transformed with epstein-barr virus. reduced numbers and normal affinity of gr were found in the epstein-barr v ... | 1989 | 2539255 |
| sister chromatid exchange and nasopharyngeal carcinoma. | sister chromatid exchange (sce) is a genetic indicator of dna damage in mammalian cells and may afford a sensitive monitor to follow genomic instability of some individuals with fragile chromosomal diseases or malignancies. in studies on the effect of dinitrosopiperazine (dnp), aflatoxin b1 (afb1), methyl-nitro-nitroso-guanidine (mnng) and epstein-barr virus (ebv) infection on sce in lymphocytes from nasopharyngeal carcinoma (npc) patients, we found that: (1) the spontaneous sces in peripheral b ... | 1989 | 2539330 |
| epstein-barr virus and a tumour-promoting phorbol ester use similar mechanisms in the stimulation of human b-cell proliferation. | in marked contrast to ligands which activate b cells via their physiological receptors for antigen, transforming epstein-barr virus (ebv) was found to be mitogenic for human b lymphocytes without increasing inositol phospholipid hydrolysis. b-cell stimulation by ebv showed similar characteristics to those achieved by the tumour-promoting phorbol ester tpa, in terms of the temporal appearance of surface activation antigens, the induction of rna and dna synthesis and the lower requirement for medi ... | 1989 | 2539333 |
| epstein-barr virus gene expression in malignant lymphomas induced by experimental virus infection of cottontop tamarins. | inoculation of cottontop tamarins with a large dose of epstein-barr virus (ebv) leads to the induction of multiple ebv genome-positive lymphomas. these tumors have been characterized as oligoclonal or monoclonal large-cell malignant lymphomas that closely resemble the ebv genome-positive b-cell lymphomas that arise in human allograft recipients. the expression of latent and lytic ebv-encoded proteins was investigated in these virus-induced tamarin lymphomas and in derived cell lines. the tamarin ... | 1989 | 2539497 |
| a study of epstein-barr virus infections in heart and heart and lung transplant recipients. | | 1989 | 2539676 |
| cotransfection of nucleic acid segments by sendai virus envelopes. | it is reported here that sendai virus envelopes (sve) can be used to transfect multiple copies of dna segments of different varieties and size. this capability further increases the usefulness of sve. in addition, the ability to simultaneously transfect multiple copies of different genome segments promises to be a powerful tool in the field of molecular biology. the simultaneous transfection of neo gene and cytomegalovirus immediate early antigen gene was successfully done. sendai virus envelope ... | 1989 | 2539842 |
| opsoclonus secondary to an epstein-barr virus infection. | | 1989 | 2540736 |
| epstein-barr virus bzlf1 trans-activator specifically binds to a consensus ap-1 site and is related to c-fos. | two regions of the epstein-barr virus bzlf1 trans-activator protein have sequence similarity to the c-fos protein. part of the similarity corresponds to the region of c-fos which is similar to the dna binding domain of c-jun and gcn-4. the structure of the exon which contains this region in c-fos and bzlf1 is also highly conserved between the two genes. complete bzlf1 protein and a c terminal fragment were prepared either as purified fusion proteins or by in vitro translation from a bzlf1 cdna. ... | 1989 | 2540954 |
| the generation of hybridomas secreting human monoclonal antibodies reactive with type ii collagen. | human monoclonal antibodies reactive with type ii collagen were obtained from patients with rheumatoid arthritis and relapsing polychondritis by fusion of cells with the human-mouse myeloma analogue hmma2.11 tg/0. direct fusion of peripheral blood or bone marrow mononuclear cells was unsuccessful in obtaining antibody producing hybridomas although fusion efficiency was high (1 hybridoma per 25,400 mononuclear cells fused). polyclonal, epstein barr virus transformed b cell lines derived from peri ... | 1989 | 2541068 |
| ebv, molecular mimicry and rheumatoid arthritis: a hypothesis. | | 1989 | 2541079 |
| dna sequence analysis of the hla-drw12 allele. | the complete dna sequence of a dr beta chain cdna encoding the drw12 allele has been determined. the sequence of this drb1 allele reveals a structural relationship to the group of other drb1 genes found on drw52 haplotypes, such as dr3, -w11, -w13, -w14, and -w8. the structural similarities among this group of alleles are particularly evident in the first hypervariable as well as in the 3' untranslated region. the second hypervariable region contains a unique sequence not identified in any other ... | 1989 | 2541120 |
| impaired late suppression of epstein-barr virus (ebv)-induced immunoglobulin synthesis: a common feature of autoimmune disease. | we examined regulation of epstein-barr virus-induced plaque-forming cell generation in peripheral blood mononuclear cells from several autoimmune and seronegative diseases and correlated these results with epstein-barr virus-induced proliferation. we confirmed the defective regulation of epstein-barr virus-induced plaque-forming cells in peripheral blood mononuclear cells of patients with rheumatoid arthritis and scleroderma. peripheral blood mononuclear cells from patients with seronegative art ... | 1989 | 2541162 |
| cytotoxic t lymphocytes specific for self tumor immunoglobulin express t cell receptor delta chain. | ctl are thought to play a role in the elimination of transformed cells in vivo. the effectiveness of such ctl is in part dependent on recognition of tumor specific antigens. among the best characterized tumor-specific antigens are the unique or idiotypic determinants on the ig of b cell lymphomas. here we describe the generation and properties of human ctl specific for the idiotype on autologous b cell tumors. these cells are cd3+,cd4-,cd8- and express the delta chain of the tcr. such cells may ... | 1989 | 2541219 |
| undifferentiated carcinoma of nasopharyngeal type of tonsil. | a review of 2262 squamous cell carcinomas of the tonsillar region seen at the institut gustave-roussy, villejuif, france, from 1970 to 1986 showed 1837 well- and poorly differentiated squamous cell carcinomas and 425 undifferentiated squamous cell carcinomas. eighteen patients with undifferentiated squamous cell carcinomas presented histologic characteristics of undifferentiated carcinomas of nasopharyngeal type. radiosensibility and radiocurability (complete sterilization with 70 gy administere ... | 1989 | 2541744 |
| epstein-barr virus small rna (eber) genes: unique transcription units that combine rna polymerase ii and iii promoter elements. | the epstein-barr virus-encoded small rna (eber) genes appear to comprise an interesting subset of class iii genes different from any previously identified, including u6 and 7sk. eber genes have functional a and b box intragenic control regions. in addition, they contain three upstream elements that together stimulate in vivo expression 50-fold and resemble sites associated with typical class ii promoters. dnaase i footprinting analyses using purified proteins or oligonucleotide competition demon ... | 1989 | 2541926 |
| occurrence of epstein-barr virus deoxyribonuclease enzyme activity-neutralizing antibodies. an index of epstein-barr virus disease. | the occurrence of epstein-barr virus (ebv) specific dnase antibodies was measured in the sera of 400 patients. three hundred sera were positive (greater than or equal to 20) for early antigen diffuse component (ea-d) antibodies, as measured by indirect immunofluorescence (if). one hundred sera were ea-d antibody-negative but had if igg titers (greater than 40) to the viral capsid antigen (vca). twenty-nine of the anti-ea-d-positive sera, and 14 of the anti-ea-d-negative sera showed specific inhi ... | 1989 | 2542178 |
| antibody to epstein-barr virus-specific dnase as a marker for field survey of patients with nasopharyngeal carcinoma in taiwan. | a serological survey using antibody to epstein-barr virus (ebv)-specific dnase activity as a marker for the identification of patients with nasopharyngeal carcinoma (npc) has been carried out on healthy subjects who visited government employees' clinic center (gecc) for routine health examination and on individuals residing in npc high-risk areas (hra) in taiwan. during a 3-year prospective study, 22,596 and 9,869 sera were collected from the gecc and hra groups, respectively. taking neutralizat ... | 1989 | 2542436 |
| heterophile igm, iga, and ige antibodies in infectious mononucleosis. | fifty infectious mononucleosis (im) and 150 non-infectious mononucleosis (non-im) sera were tested by a hemadsorption immunocapture test (hit) for the detection of heterophile antibodies of igm, iga, and ige classes. the specificity of paul-bunnell (pb) antibodies was ascertained by a differential absorption test. igm pb-antibodies were demonstrated in 100% of im sera, iga in 92%, and ige in 88%. pb antibodies were present only in im sera; but other heterophile antibodies of igm (68%), iga (6.7% ... | 1989 | 2542444 |
| the transforming domain alone of the latent membrane protein of epstein-barr virus is toxic to cells when expressed at high levels. | a previously unrecognized activity has been associated with the product of the bnlf-1 gene of epstein-barr virus. this gene encodes the latent membrane protein of epstein-barr virus. when the gene was expressed at high levels, it was toxic to all cell lines tested, which included six human b-lymphoid lines as well as balb/3t3, 143/ebna-1, and hep-2 cells. the bnlf-1 gene was previously shown to induce anchorage-independent and tumorigenic growth in rat-1 and balb/3t3 cells. we demonstrate here t ... | 1989 | 2542565 |
| a promoter of epstein-barr virus that can function during latent infection can be transactivated by ebna-1, a viral protein required for viral dna replication during latent infection. | a viral promoter that functions on recombinant plasmids in cells immortalized by epstein-barr virus was identified and characterized. it is identical to that mapped on the viral genome by bodescot et al. (m. bodescot, m. perricaudet, and p.j. farrell, j. virol. 61:3424-3430, 1987) which functions during the latent phase of the viral life cycle in some but not all cells to encode several latent viral gene products. experiments with these plasmids indicated that this promoter requires the enhancer ... | 1989 | 2542577 |
| host cell-dependent regulation of growth transformation-associated epstein-barr virus antigens in somatic cell hybrids. | we have analyzed the expression of the three major known growth transformation-associated epstein-barr virus (ebv) proteins, ebna-1, ebna-2, and latent membrane protein (lmp), in a series of somatic cell hybrids derived from the fusion of ebv-carrying burkitt lymphoma (bl) lines with ebv-positive or ebv-negative b-cell lines. independently of the cell phenotype, ebna-1 was invariably coexpressed in all ebv-carrying hybrids. in hybrids between ebv-carrying, lmp-positive and lmp-negative burkitt l ... | 1989 | 2542588 |
| [structure and functions of epstein-barr virus genomes]. | | 1989 | 2542651 |
| [diagnosis of ebv infections using an immunologic method]. | | 1989 | 2542659 |
| [eb virus-associated tumors-opportunistic b cell lymphoma, burkitt's lymphoma, nasopharyngeal carcinoma]. | | 1989 | 2542671 |
| oral hairy leukoplakia: diagnosis and management. | oral hairy leukoplakia (hl) is a remarkable lesion associated with epstein-barr virus (ebv) found in persons infected with the human immunodeficiency virus. the clinical and histologic features of hl are characteristic and distinctive. however, none of these features are entirely specific for hl, and we consider that the presence of ebv is required for diagnosis in questionable cases. ebv can be demonstrated by means of electron microscopy, immunocytochemistry, or molecular biologic techniques. ... | 1989 | 2542857 |
| assessment of responses of normal human b lymphocytes to different isolates of human immunodeficiency virus: role of normal donor and of cell line used to prepare viral isolate. | the effect of different hiv-1 isolates on normal human b lymphocyte function has been studied in vitro. production of igm and igg was measured by elisa using a "standard" non-t preparation of b cells depleted of macrophages and t cells (but not of low-density accessory cells, ldc). only one (h9/cbl-4) of five different isolates induced polyclonal production of immunoglobulin. apart from intrinsic differences between isolates, important inherent variables were shown to affect the response. one wa ... | 1989 | 2543435 |
| an epstein-barr virus-transformed b cell line produces autoregulatory interleukin-1 that regulates bone remodeling. | in this study, we demonstrate that an epstein-barr virus-transformed b cell line, a-11, produced interleukin-1 (il-1), a cytokine that regulates bone remodeling. a-11 cells produce il-1 in a cell dose- and culture time-related manner. the il-1 activity was neutralized by recombinant human il-1 (rhil-1) alpha antiserum, but not by rhil-1 beta antiserum. the il-1 was semi-purified by (nh4)2so4 precipitation, superose prep 12 gel filtration, and anion-exchange chromatography strongly stimulated in ... | 1989 | 2543455 |
| human ige response: virus-activated ige secretors are interleukin-2-dependent cells. | the activation of human ige-secreting b lymphocytes was difficult until the use of low cell density limiting dilution cultures, which are more efficient and eliminate regulatory growth restraints, became possible. the transformation of ige-producing cells by epstein-barr virus unexpectedly required the presence of small supplements of mature t lymphocytes early in culture. interleukin 2 (il2, 10 u/ml) was able to completely replace t-cell supplements when added during a brief (1-2 cell cycles) b ... | 1989 | 2543638 |
| relationship between clinical stage, histopathology and antibody titers against the second epstein-barr virus nuclear antigen (ebna-2) in non-hodgkin's lymphoma patients. | non-hodgkin lymphoma (nhl) patients with centroblastic (cb) or centroblastic-centrocytic (cb/cc)-diffuse lymphomas, immunocytoma (ic) and chronic lymphocytic leukemia (cll) in clinical stages iii-iv and with active disease (highly malignant group) were compared to nhl patients with cll, ic, and centrocytic (cc) or centroblastic-centrocytic (cb-cc)-diffuse/follicular lymphomas, in clinical stages i-ii and with inactive disease (low malignant group) based on the presence of antibodies to epstein-b ... | 1989 | 2543639 |
| susceptibility to azt of hiv-1 variants grown in epstein-barr virus-transformed b cell lines. | how 3'-azido-3'-deoxythymidine (azt) affects the ability of three different variants of hiv-1 to infect a line of ebv-transformed b cells was studied. susceptible cells were pretreated with three different concentrations of azt (1, 5, and 10 microm) for 4 h before viral inoculation and were maintained after infection in drug-containing medium. establishment of viral infection was assessed by indirect immunofluorescence for viral antigens and by antigen capture assay carried out on culture fluids ... | 1989 | 2543710 |
| [studies on the levels of specific antibodies to epstein-barr virus and heterophile antibody in the patients with infection mononucleosis]. | | 1989 | 2543850 |
| growth- and tumor-promoting effects of deregulated bcl2 in human b-lymphoblastoid cells. | human follicular b-cell lymphomas possess a t(14;18) that translocates a putative protooncogene, bcl2, into the immunoglobulin heavy chain locus. the normal bcl2 gene is quiescent in resting b cells, expressed in proliferating, but down-regulated in differentiated b cells. inappropriately high levels of bcl2-immunoglobulin chimeric rna are present in t(14;18) lymphomas for their mature b-cell stage. we examined the biologic effects of bcl2 deregulation in human b cells by introducing bcl2 into h ... | 1989 | 2543982 |
| repair of 8-methoxypsoralen + uva-induced damage in specific sequences in chromosomal and episomal dna in human cells. | a study of the repair of dna damage in the dihydrofolate reductase (dhfr) gene of sv40-transformed human fibroblasts after treatment with 8-methoxypsoralen (8mop) and uva is described. 8mop+uva-induced cross-links in the dhfr gene were completely repaired by 12 h in one normal and one fanconi's anaemia (fa) group a cell line. in contrast, approximately 35% of cross-links in an episomally maintained epstein--barr virus derived plasmid remained unrepaired even after 48 h. cross-linkable monoadduct ... | 1989 | 2544311 |
| epstein-barr virus latent gene expression during the initiation of b cell immortalization. | epstein-barr virus (ebv) has the capacity to immortalize a subpopulation of resting b lymphocytes. lymphoblastoid cell lines (lcl) established in this way carry the latent ebv genome as multiple copies of an extrachromosomal episome. viral gene expression in lcls is highly restricted; products identified correspond to a membrane protein (latent membrane protein; lmp), a nuclear antigen complex (epstein-barr nuclear antigens; ebnas 1 to 6), two small rna species (ebers 1 and 2) and rna species th ... | 1989 | 2544663 |
| antibody against epstein-barr virus dna polymerase activity in sera of patients with nasopharyngeal carcinoma. | a salt-dependent dna polymerase activity was demonstrated in the culture of an ebv-producing, lymphoblastoid cell line (npc-204 cells) treated with 5-iodo-2'-deoxyuridine (iudr). there was a high frequency of levels of antibody to this enzyme in sera of patients with nasopharyngeal carcinoma (npc). in contrast, sera from healthy subjects had little or no neutralizing activity. the high antibody level appeared as early as stage 1 of the disease in many npc patients. the levels of the antibody inc ... | 1989 | 2544674 |
| human herpesvirus epstein-bar virus: ubiquity, oncogenicity, and risk factors. | | 1989 | 2544754 |
| [vascular purpura and other cardiovascular alterations in children with acute parvovirus b19 infection. report of 3 personal cases]. | most frequent skin manifestation of parvovirus b19 infection is the erythema infectiosum (fifth disease; sticker's disease). vascular purpura (some cases of henoch-schönlein purpura) has been reported in virus infections such as infectious mononucleosis (epstein-barr virus), hepatitis b, cytomegalovirus etc., and human parvovirus b19 could be another cause. clinical course, procedure of diagnostics and therapy in 3 own serologically established cases of vascular purpura or severe alterations of ... | 1989 | 2544763 |