| in vitro and in vivo characterization of pyocin. | pyocin, a bacteriocin obtained from lysates of ultraviolet-induced cultures of pseudomonas aeruginosa was characterized in vitro and in vivo after 1,000-fold purification by chemical, column, and differential centrifugation procedures. electron micrographs of negatively stained pyocin preparations contained rod-shaped particles which resembled the contractile tail protein of the t-even phages of escherichia coli. although two separate and distinct pyocin fractions were eluted from diethylaminoet ... | 1967 | 4960899 |
| comparative sedimentation rates of plant, bacterial and animal ribosomal rna. | | 1967 | 4962194 |
| comparative studies of antigens from mycoplasma mycoides and escherichia coli. | extracts of sonically disrupted mycoplasma mycoides and escherichia coli were fractionated by sucrose density gradient centrifugation. the presence of antigen in each of the fractions was determined by complement-fixation and agar-gel diffusion precipitin tests, in which cow, pig, and rabbit anti-m. mycoides sera and rabbit anti-e. coli serum were used. fractions of m. mycoides, with a buoyant density of 1.225 or lower, fixed complement with cow and pig anti-m. mycoides sera. these fractions als ... | 1968 | 4967193 |
| immunological studies of aspartate transcarbamylase. i. characterization of the native enzyme, catalytic, and regulatory subunit immune systems. | | 1968 | 4973476 |
| cross-reacting material produced by lac deletion mutants of escherichia coli k12. | | 1969 | 4974834 |
| l-rhamnulose 1-phosphate aldolase from escherichia coli. crystallization and properties. | | 1969 | 4975916 |
| increased host resistance to infection elicited by lipopolysaccharides from brucella abortus. | | 1969 | 4980494 |
| studies on a lipopolysaccharide from escherichia coli. heterogeneity and mechanism of reversible inactivation by sodium deoxycholate. | | 1969 | 4981348 |
| the antigenic independence of the three aspartokinases of escherichia coli k 12. | | 1969 | 4982820 |
| the antibody response in rabbits to e. coli k antigen. | | 1970 | 4983348 |
| studies on swine serum immunoglobulins. ii. relationship between immunoglobulins and antibodies. | | 1969 | 4985397 |
| terpene compounds as drugs. 8. bacteriological and pharmacological properties of terpenyl penicillins. | | 1970 | 4986283 |
| [stimulation of antibodies to pathogenic microorganisms in animals by immunizing them with saprophytes]. | | 1969 | 4986751 |
| the generalized shwartzman phenomenon in rabbits with denervated kidneys. | | 1970 | 4989764 |
| [anti-tumor activity and side reaction of bacterial component]. | | 1970 | 4990957 |
| chemical composition of lipopolysaccharide from an avian strain of escherichia coli o18. | | 1970 | 4992532 |
| amount, class and specificity of antibody to the lipopolysaccharide of salmonella enteritidis after immunization by various schedules. | | 1971 | 4993854 |
| pathogenesis of escherichia coli diarrhea. | | 1971 | 4996788 |
| apparent serologic reaction between bacterial cell components and blood components. | | 1971 | 4999475 |
| l-asparaginase from proteus vulgaris. | to produce an immunologically and enzymologically new type of l-asparaginase, 108 strains of bacteria were screened for enzyme production. as a result, 13 bacteria belonging to the genera alcaligenes, bacterium, and proteus were found to produce l-asparaginases in high levels. among these l-asparaginases, partially purified l-asparaginases from b. cadaveris and p. vulgaris showed antitumor activity. a partially purified l-asparaginase preparation of p. vulgaris did not react with the antibody of ... | 1971 | 5000866 |
| temperature optimum of alkaline phosphatases in some homeothermic and poikilothermic species. | | 1971 | 5000930 |
| hexose-atp phosphotransferases: comparative aspects. ii. cross-reactivity of hexokinases from a variety of species with antisera to yeast hexokinase. | | 1971 | 5001183 |
| platelet interaction with bacteria. ii. fate of the bacteria. | several common strains of bacteria have been studied to determine their influence on human and rabbit platelets in vitro. bacteria at a nominal ratio of 1:1 were added to platelets in their native plasma or to platelets in a balanced salt solution. the platelet-bacterial interaction was examined by nephelometry and by sequential light microscopy of fixed specimens taken at key points in the reaction. the results support the following conclusions: (1) bacteria that were potent stimuli of the plat ... | 1971 | 5002629 |
| the rapid detection and determination of sparse bacterial populations with radioactively labelled homologous antibodies. | | 1971 | 5002928 |
| [microbiological relationships between man and laboratory animals]. | | 1971 | 5004293 |
| localization and biological and physicochemical properties of the cell wall lipopolysaccharide of rhodopseudomonas capsulata. | electron micrographs of phenol-water-extracted lipopolysaccharide (lps) of rhodopseudomonas capsulata show filamentous and netlike aggregates. treatment of the lps with sodium deoxycholate resulted in a reversible splitting into subunits. the lps represents a cell wall constituent with o-antigenic specificity. in passive hemagglutination tests, high titers were obtained when erythrocytes sensitized with untreated or heat-treated lps were incubated with antisera obtained by immunization of rabbit ... | 1972 | 5018024 |
| the antigenic structure of lambda bacteriophage. | | 1965 | 5319343 |
| the behaviour of platelets in vivo. | | 1965 | 5321788 |
| [research on antigenic cross-reactivity between blood group substances and enterobacteria]. | | 1965 | 5322443 |
| in vitro inhibition of leukocyte uptake of radioactive endotoxin by components of normal serum. | | 1966 | 5327112 |
| the secondary structure of ribosomal ribonucleic acid in solution. | 1. the u.v.-absorption spectrum of ribosomal rna from rabbit reticulocytes was studied as a function of temperature at different ph values. the changes in the spectrum over the range 220-320mmu were interpreted on the basis of the assumption that the effect of denaturation and ionization are additive. the results suggest that in neutral salt solutions the secondary structure of the ribosomal rna samples studied is due to two species of helical segments stabilized principally, if not solely, by c ... | 1966 | 5330109 |
| the action of chloroform-killed suspensions of enteropathogenic escherichia coli on ligated rabbit-gut segments. | | 1966 | 5330337 |
| comparisons of antibodies reacting with dna. ii. rabbit antibodies induced by nucleoside-protein conjugates. | | 1966 | 5330580 |
| bactericidal antibody in mammalian serum to obligatorily anaerobic gram-negative bacteria. | | 1966 | 5331727 |
| the optical rotatory dispersion of ribosomes and their constituents. | | 1966 | 5334795 |
| effect of trypsin on escherichia coli and rabbit reticulocyte ribosomes. | | 1966 | 5336065 |
| electron microscopy of the effect of gram-negative endotoxin on the blood-brain barrier. | | 1966 | 5336208 |
| dissociation of antitoxic and immunogenic activities of endotoxin by acetylation. | | 1966 | 5336352 |
| an indirect fluorescent antibody study of vibrio comma. | | 1966 | 5336437 |
| heterogeneity of rabbit serum interferons. | | 1966 | 5338578 |
| immunological priming without production of circulating bacterial antibodies conditioned by endotoxin and its lipoid a component. | | 1966 | 5339135 |
| production of generalized shwartzman reaction in rabbits with nitrogen mustard-induced granulocytopenia. | | 1967 | 5339203 |
| quantitative aspects of blood coagulation in the generalized shwartzman reaction. ii. effect of cortisone. | | 1967 | 5339831 |
| glyceraldehyde-3-phosphate dehydrogenase variants in phyletically diverse organisms. | electrophoretically distinct forms of glyceraldehyde-3-phosphate dehydrogenase (tdh) have been detected in turtle, perch, trout, spinach, and yeast. multiple forms were not detected in rat, rabbit, chicken, frog, honey bee, euglena, or escherichia coli. the combination of two different subunits into tetramers is a probable explanation for the five-membered sets usually detected in extracts exhibiting tdh multiplicity. | 1967 | 5341534 |
| effect of bacterial lipopolysaccharide on the content of serotonin and norepinephrine in rabbit brain. | | 1966 | 5342289 |
| escherichia coli k bacteriophages. i. isolation and introductory characterization of five escherichia coli k bacteriophages. | a set of five escherichia coli k phages has been isolated. these phages are adsorbed to and lyse the capsular forms of the host bacteria, whereas their spontaneous, acapsular mutants are not affected. all host strains are heavily encapsulated test strains for e. coli k antigens of the thermostable a type and they readily segregate acapsular mutants. in four of the phage-host systems, all secondary growth obtained was found to be acapsular. when tested for host-range mutants on 38 strains of e. c ... | 1968 | 5723715 |
| identification of gag and env gene products of human t-cell leukemia virus (htlv). | the gag and env gene products of human t-cell leukemia virus (htlv) were identified with rabbit antisera against the synthetic peptides and a polypeptide produced in escherichia coli, which corresponded to parts of the proteins predicted from the nucleotide sequence of htlv [m. seiki, s. hattori, y. hirayama, and m. yoshida (1983). proc. natl. acad. sci. usa 80, 3618-3622]. viral proteins were detected by immunoprecipitation in two htlv-producing cell lines. the precursor of gag products was a p ... | 1984 | 6087548 |
| protein products of the bithorax complex in drosophila. | a sequence from the ubx 5' exon in the bithorax complex of drosophila melanogaster was expressed as a fusion protein in bacteria. this protein was used to raise rabbit antisera and monoclonal antibodies. these antibodies detect antigens that, on protein blots and by immunofluorescence on whole mounts of imaginal discs, show the predicted segmental distribution of ubx products. these products are predominantly, if not totally, localized in the cell nucleus. in the embryonic nervous system nuclei ... | 1984 | 6091908 |
| a membrane protein encoded by epstein-barr virus in latent growth-transforming infection. | the nucleotide sequence of an epstein-barr virus gene expressed in latently infected growth-transformed cells is known to include a long open reading frame containing a 33-base-pair repeat element. a bacterial fusion protein constructed from a portion of the reading frame and escherichia coli beta-galactosidase was used to produce sera in rabbits against the previously unidentified gene product. the viral protein detected with these sera in latently infected cells varies in size with the number ... | 1984 | 6095274 |
| infectious agents associated with diarrhoea in commercial rabbits: a field study. | over a period of 9 months the occurrence of parasites, bacteria and viruses has been examined in diarrhoeic rabbits from 21 commercial rabbitries and related with clinical signs and gross and microscopic lesions. infectious disease agents have been found in 71.5% of the animals. escherichia coli (atec) were found to be attached to the luminal intestinal border of 40% of the rabbits examined. this was associated with moderate to high mortality, caecal oedema, severe swelling of mesenteric lymph n ... | 1984 | 6097152 |
| activation of intestinal guanylate cyclase by heat-stable enterotoxin of escherichia coli: studies of tissue specificity, potential receptors, and intermediates. | heat-stable enterotoxin (st) of escherichia coli increased guanylate cyclase activity in homogenates of rat and rabbit intestinal mucosa and stimulated intestinal fluid secretion in suckling mice. the st effect on guanylate cyclase was dose-dependent, occurred without a time lag, and was confined to the particulate fraction. st activation of guanylate cyclase was tissue-specific; st did not alter activity of soluble or particulate rat liver, lung, heart, kidney, or cerebral cortex enzyme. the st ... | 1980 | 6106030 |
| enteropathogenicity: recent developments. | recent studies have added important new information to our understanding of the pathogenesis and ethiology of diarrheal disease. vibrio cholerae produces a heat-labile enterotoxin, affecting cyclic amp. a very similar heat-labile enterotoxin is produced also by certain strains of escherichia coli, as well as by citrobacter, klebsiella, and aeromonas. e. coli may also produce a heat-stable enterotoxin, stimulating guanylate cyclase activity. in order to produce the pathologic effects, e. coli fir ... | 1982 | 6126611 |
| adhesion inhibition by antibodies. | the barrier function of the mucous membranes is exerted through unspecific defense mechanisms like mechanic washing and by specific immunity. antibodies in secretion do not mainly act through bactericidal mechanisms, but rather by binding to antigens, neutralizing, agglutinating and immobilizing them. the net result will be a decreased chance for bacteria to reach and bind to host epithelial cells. antibodies directed against bacterial adhesions will specifically interfere with the adhesion proc ... | 1982 | 6127804 |
| specific adherence of escherichia coli (strain rdec-1) to membranous (m) cells of the peyer's patch in escherichia coli diarrhea in the rabbit. | the rdec-1 strain escherichia coli is an enteroadherent bacterium that produces diarrhea in the rabbit. a histopathologically similar disease has been described in humans. the rdec-1 bacterium adheres to the epithelium of lymphoid follicles in rabbit ileal peyer's patches by 4 h postinoculation, 3-4 d before its adherence to absorptive epithelium. the purpose of this study was to determine whether the rdec-1 bacterium adheres to a specific cell type in the lymphoid follicle epithelium. rdec-1 ba ... | 1983 | 6129261 |
| stimulation of polymorphonuclear leucocyte phospholipase a2 activity by chloroquine and mepacrine. | | 1982 | 6129307 |
| receptor analogues and anti-pili antibodies as inhibitors of bacterial attachment in vivo and in vitro. | | 1983 | 6135384 |
| monoclonal antibodies against colonization factor antigen i pili from enterotoxigenic escherichia coli. | hybridomas secreting monoclonal antibodies directed against intact colonization factor antigen i pili have been produced by the fusion of spleen cells from immunized balb/c mice with ns1/sp2 myeloma cells. the four monoclones with the highest antibody titer, as detected by enzyme-linked immunosorbant assay (elisa), were chosen for antibody amplification by production of mouse ascitic fluid. these four were examined for antibody specificity by elisa and immunoblot assays, using six different pilu ... | 1983 | 6136463 |
| genetics of digalactoside-binding adhesin from a uropathogenic escherichia coli strain. | the uropathogenic strain escherichia coli j96 mediates mannose-resistant hemagglutination owing to production of a digalactoside-binding adhesin. a cosmid clone from this strain has been isolated that, when harbored in e. coli k-12, expressed pap pili and this adhesin (r. hull et al., infect. immun. 33:933-938, 1981). by transposon mutagenesis and by the construction of a number of hybrid plasmid derivatives, we have demonstrated that about 8.5 kilobases of dna is required to generate a mannose- ... | 1983 | 6136465 |
| in vitro biosynthesis and membrane insertion of gamma-glutamyl transpeptidase. | gamma-glutamyl transpeptidase consists of two polypeptide chains anchored to the kidney brush-border membrane only through a short hydrophobic domain near the nh2-terminal end of the heavy subunit. the two subunits were reported to derive from a single polypeptide precursor by tissue labeling experiments. we have investigated the first steps of ggt biosynthesis and processing in a cell-free system. mrna was prepared from kidney and enriched in specific sequences by a preparative gel electrophore ... | 1984 | 6143750 |
| a virulence plasmid in escherichia coli enterotoxigenic for humans: intergenetic transfer and expression. | we studied the colonization-factor antigen i (cfa/i) fimbriae- and heat-stable enterotoxin (st)-coding plasmid of enterotoxigenic escherichia coli (strain h10407, serotype o78:h11) pathogenic for humans. with use of conjugal-transfer system of e. coli h10407 and transposon-labeling techniques, the virulence plasmid was shown to be transferable to many species of the family enterobacteriaceae, including the enteropathogens, shigella and salmonella species, and the opportunistic pathogens, klebsie ... | 1984 | 6149247 |
| fimbria-like hemagglutinin of escherichia coli o75 strains. | fifteen strains of escherichia coli o75 from human feces and patients with urinary tract infections were analyzed for their hemagglutinative properties, production of hemolysin and colicin, and plasmid contents. fourteen strains produced type-1 fimbriae in broth culture. nine of the strains agglutinated human p1 and p erythrocytes, i.e., possessed an x adhesin (x hemagglutinin). all but one of the x+ strains agglutinated human but not sheep or rabbit erythrocytes. of the 15 strains, 4 had p fimb ... | 1984 | 6150006 |
| bacterial cloning of the rabbit uteroglobin structural gene. | | 1980 | 6156676 |
| rna-binding activity of eukaryotic initiation factors of translation. | | 1980 | 6157563 |
| international conference on byssinosis. mechanisms of disease induction. | from this work and other published data there are at least three distinct compounds that have been shown to be capable of inducing symptoms of byssinosis. there is an aminopolysaccharide-protein complex in cotton plant bracts that acts by causing histamine release in the human lung and also causes necrosis of bronchiolar epithelium. there is endotoxin or endotoxin-like material present in cotton plant bracts that acts by a mechanism other than by causing histamine release. this can induce histol ... | 1981 | 6162621 |
| selective inhibition of escherichia coli protein synthesis and growth by nonionic oligonucleotides complementary to the 3' end of 16s rrna. | a series of nonionic oligonucleotide analogues, the deoxyribooligonucleoside methylphosphonates, were synthesized. the base sequences of these compounds, d(apgpgp), d(apgpgp)(2), and d[(apgpgp)(2)t], are complementary to the shine-dalgarno sequence (-a-c-c-u-c-c-u-) found at the 3' end of bacterial 16s rrna. these nonionic oligonucleotide analogues were tested for their ability to inhibit the in vitro translation of mrnas in cell-free systems of escherichia coli and rabbit reticulocyte. in the e ... | 1981 | 6165012 |
| immunological properties of escherichia coli heat-stable enterotoxins: development of a radioimmunoassay specific for heat-stable enterotoxins with suckling mouse activity. | antiserum was raised against the purified heat-stable enterotoxin (st) produced by enterotoxigenic escherichia coli strain 431, a class ii porcine enteropathogen. the antiserum was used to examine the antigenic determinants of sts produced by enterotoxigenic strains of different host origins and develop a sensitive radioimmunoassay specific for st having biological activity in suckling mice and piglets (sta). the antiserum neutralized one effective dose of toxin at a dilution of 1:5,000 and neut ... | 1981 | 6167519 |
| production of immunologically active surface antigens of hepatitis b virus by escherichia coli. | several plasmids have been constructed which direct the synthesis of hepatitis b virus surface antigens in escherichia coli either as the native polypeptide or fused to other plasmid encoded polypeptides. when injected into rabbits, extracts from bacteria carrying some of these plasmids induced the synthesis of antibodies to the antigens even though the extracts did not give satisfactory positive results in radioimmunoassay for them. either the nh2-terminal segment or the cooh-terminal segment o ... | 1981 | 6170067 |
| [method of determining microorganism-induced leukocyte lysozyme activity]. | | 1981 | 6170822 |
| comparative study of escherichia coli endotoxin, hydrocortisone and beljanski leukocyte restorer activity in cyclophosphamide-treated rabbits. | | 1981 | 6172795 |
| ribosome structure. localization of 7-methylguanosine in the small subunits of escherichia coli and chloroplast ribosomes by immunoelectron microscopy. | the minor nucleoside 7-methylguanosine occurs in escherichia coli 16 s ribosomal rna at a single site. high pressure liquid chromatographic analysis shows that a single residue of 7-methylguanosine is also present in chloroplast 16 s ribosomal rna, presumably at an analogous position in the sequence. antibodies to 7-methylguanosine were induced in rabbits and shown to be highly specific for the intact methylated base. these antibodies were reacted with 30 s ribosomal subunits from e. coli and fr ... | 1982 | 6179936 |
| identification of salmonellae of serogroup c1 by immunofluorescence and co-agglutination with antiserum against an oligosaccharide-protein conjugate. | antiserum specific for salmonella o7 antigen raised by immunisation of rabbits with an artificial conjugate consisting of oligosaccharide and bovine serum albumin (os-bsa). the oligosaccharide was a pentasaccharide isolated after cleavage of the o antigen polysaccharide chain of salmonella thompson (o antigen 6, 7) with endo-glycanase from bacteriophage 14. the usefulness of the s. thompson os-bsa antiserum for rapid and accurate identification of isolates of salmonella of serogroup c1 (o6, 7) w ... | 1982 | 6183432 |
| biosynthesis of preprorenin. studies using whole tissue, a cell-free system, and e. coli containing cdna inserted at the psti site of plasmid pbr322. | the biosynthesis of renin as a higher molecular weight 'prorenin' was demonstrated by in vitro incorporation of [35s] methionine into nascent polypeptides of submandibular gland tissue from adult male mice. immunoprecipitation with anti-renin and electrophoresis identified a mr 44,500, pi 6.4 prorenin which normally represented 5% of renin-immunoreactive protein in tissue extracts and which was rapidly converted during in vitro labeling into a mr 40,000, pi 6.2 species. the latter was subsequent ... | 1982 | 6184188 |
| antibody-dependent cell-mediated cytotoxicity against escherichia coli o antigens. | antibodies against escherichia coli o antigen from rabbits immunized with formalin-killed bacteria were tested for cytotoxic capacity in an antibody-dependent cell-mediated cytotoxicity (adcc) assay with human lymphocytes as effector cells and autologous papainized erythrocytes coated with o antigen as target cells. the cytotoxic titres were compared with the titres obtained with three methods of antibody quantitation. it was found that adcc recorded antibodies with similar sensitivity as the en ... | 1982 | 6184220 |
| immunization with rough mutants of salmonella minnesota. iv. protection by antisera to o and rough antigens against endotoxin. | the protection by antisera to o antigens and antigens (lipid a, the re 595 mutant of salmonella minnesota, and the j5 mutant of escherichia coli) of the core portion of endotoxin against lethal challenge with lipopolysaccharide (lps) was compared. rabbits immunized with the re mutant developed antibody that protected mice against challenge with s. minnesota or salmonella typhosa lps. antisera to heterologous o antigen and lipid a were not protective, whereas homologous antisera and antiserum to ... | 1983 | 6185599 |
| detection of fungemia obscured by concomitant bacteremia: in vitro and in vivo studies. | our recent clinical experience suggested that bacteremia may interfere with the detection of concomitant fungemia when standard blood culture methods are used. to determine the extent to which bacteria may interfere with fungal isolation from blood cultures, an in vitro model simulating blood cultures taken during concomitant fungemia and bacteremia was created. each of six bacteria (staphylococcus epidermidis, staphylococcus aureus, streptococcus faecalis, escherichia coli, klebsiella pneumonia ... | 1982 | 6186687 |
| antibodies to escherichia coli k and o antigens in protection against acute pyelonephritis. | | 1983 | 6187016 |
| leukocyte recovery with short-chain rna fragments in cyclophosphamide-treated rabbits. | single-stranded short-chain rna fragments, obtained by mild degradation of purified escherichia coli ribosomal rna(s) with pancreatic rnase a, exhibit particular biologic activities in vitro and in vivo. in vitro, these rna fragments are used by dna-dependent dna polymerase i as primers to initiate the replication of dna(s) isolated from rabbit bone marrow and spleen; they are inactive with dna isolated from several normal tissues and cancerous cells. administered iv, rna fragments restore a nor ... | 1983 | 6191862 |
| immunopathogenesis of chronic pyelonephritis. | immunopathologic responses to urinary tamm-horsfall protein in the development of chronic pyelonephritis were examined by four different approaches. first, in a rabbit model, tubulointerstitial nephritis developed in 64 of 102 rabbits injected intravenously with urine or rabbit tamm-horsfall protein as compared with only one of 17 rabbits in two control groups. circulating cytotoxic lymphocytes plus immunoglobulin g (igg) antibodies against tamm-horsfall protein were found in 51 percent of chall ... | 1983 | 6192715 |
| characterization of new hydrophobic pili of human enterotoxigenic escherichia coli: a possible new colonization factor. | enterotoxigenic escherichia coli strains were divided into five groups on the basis of their bacterial surface hydrophobicity (honda et al., fems microbiol. lett. 17:273-276, 1983). strains in group iii showed heat-stable high hydrophobicity, although they did not show mannose-resistant hemagglutination with either human or bovine erythrocytes. e. coli strain 260-1 in group iii was characterized. electron microscopic examination revealed the presence of pili on the surface of this strain, but no ... | 1984 | 6199307 |
| sequencing of laminin b chain cdnas reveals c-terminal regions of coiled-coil alpha-helix. | cdnas for laminin b chains have been isolated from a parietal endoderm cdna library in puc8 and puc9. identification is based on: ability to direct the synthesis in escherichia coli of polypeptides carrying laminin antigen determinants, in vitro translation of hybrid selected mrna, and hybridization to high mol. wt. rna differentially expressed in cells synthesizing large amounts of laminin. the plasmid ppe9 hybrid selects mrna for the b2 (mol. wt. 185 000) chain and provides 217 residues of c-t ... | 1984 | 6209134 |
| effect of hormonal deprivation on the bladder defense mechanism. | the mucopolysaccharide (mps) layer of the bladder has been implicated as a bacterial anti-adherence factor in previous experimentation. production of mps is known to be related to hormones in other systems of the body and probably to the urinary system. due to the known rising incidence of urinary tract infection in most menopausal females, an experimental model using oophorectomized rabbits was designed. oophorectomized and control rabbits were observed for their ability to clear their bladder ... | 1982 | 6211553 |
| norfloxacin penetration into subcutaneous tissue cage fluid in rabbits and efficacy in vivo. | the activity of norfloxacin was studied in vivo with steel net cages implanted subcutaneously in rabbits. four weeks after implantation, two of four cages in each animal were inoculated with a strain of escherichia coli (seven animals) or klebsiella pneumoniae (six animals). four animals in each group received oral treatment with norfloxacin for 7 days. treatment was started 18 h after inoculation of the cages. peak concentrations above the in vitro minimal inhibitory concentrations for the stra ... | 1983 | 6221689 |
| nuclear genes coding the yeast mitochondrial adenosine triphosphatase complex. isolation of atp2 coding the f1-atpase beta subunit. | a yeast nuclear pet mutant of saccharomyces cerevisiae lacking any detectable mitochondrial f1-atpase activity was genetically complemented upon transformation with a pool of wild type genomic dna fragments carried in the yeast escherchia coli shuttle vector yep 13. plasmid-dependent complementation restored both growth of the pet mutant on a nonfermentable carbon source as well as functional mitochondrial atpase activity. characterization of the complementing plasmid by plasmid deletion analysi ... | 1983 | 6225776 |
| improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit beta-globin. | in this paper we describe a method for constructing e. coli plasmids that direct efficient expression of genes that encode eucaryotic or procaryotic proteins. no functional assays for the proteins are needed, and they are produced in their native, unfused state. the only requirement is that the genes be isolable without intervening sequences. we describe as an example the construction of a plasmid that directs the synthesis of about 10,000-15,000 monomers per cell of rabbit beta-globin. the esse ... | 1980 | 6248249 |
| mouse actin messenger rnas. construction and characterization of a recombinant plasmid molecule containing a complementary dna transcript of mouse alpha-actin mrna. | | 1981 | 6256358 |
| pharmacokinetics and bacteriologic efficacy of moxalactam, cefotaxime, cefoperazone, and rocephin in experimental bacterial meningitis. | the pharmacokinetics and bacteriologic efficacy of four beta-lactam antibiotics were studied in the serum and cerebrospinal fluid (csf) of rabbits using two test strains, escherichia coli type k1 and group b streptococcus type iii, and 24 gram-positive isolates. although moxalactam, cefotaxime, cefoperazone, and rocephin are active in vitro against gram-negative bacilli, the gram-positive bacteria listeria monocytogenes and streptococcus faecalis were resistant to all four drugs; group b strepto ... | 1981 | 6260870 |
| cdna clone coding for part of a mouse h-2d major histocompatibility antigen. | mrna coding for mouse major transplantation antigens of the d haplotype was partially purified, copied into double-stranded cdna, and cloned in escherichia coli. clones were selected by their ability to hybridize specifically with mrna coding for h-2k, d, or l antigens. one of these clones, ph-2d-1, carries a 1200-base-pair insert, comprising the noncoding region, including poly(a) at the 3' end and part of the coding region. a partial sequence of the latter region showed extensive homology with ... | 1981 | 6265910 |
| the long terminal repeat of moloney sarcoma provirus. | | 1981 | 6266761 |
| influence of bacterial endotoxins on neutrophilic leukocytes: lack of a correlation between in vivo and in vitro responses. | | 1981 | 6269119 |
| mosaic structure and mrna precursors of uteroglobin, a hormone-regulated mammalian gene. | the synthesis of uteroglobin in the rabbit uterus is induced by progesterone and is repressed by estrogen which has an over-riding effect over the inducer. the dual hormonal control system offers an excellent model for studying hormonal regulation of mammalian gene expression. using a full-length uteroglobin cdna clone as a specific hybridization probe, recombinant lambda phages containing the entire chromosomal uteroglobin gene have been isolated from a rabbit genomic dna library. electronmicro ... | 1981 | 6271764 |
| cgmp modulation of ileal ion transport: in vitro effects of escherichia coli heat-stable enterotoxin. | diarrheagenic strains of escherichia coli have been shown to produce a heat-stable enterotoxin (st) that simulates guanylate cyclase, increases short-circuit current (isc), and inhibits active cl absorption in the intestine. in rabbit ileum, the ion transport effects are smaller than those produced by camp-related agonists. because st may be a selective cgmp agonist, we further explored its mode of action in rabbit ileum. st inhibits net na and net cl absorption. st also inhibits the same fracti ... | 1982 | 6283916 |
| construction and characterization of sv40 recombinants with beta-globin cdna substitutions in their early regions. | a cdna segment coding for rabbit beta-globin has been inserted at different locations in the early region of simian virus 40 (sv40). the inserted sequences in these recombinants are transcribed from the sv40 early region promoter, and the primary transcripts are processed to mature mrnas using viral intervening sequences and the early region polyadenylation site. after infection with various recombinants, beta-globin polypeptide is synthesized only when the beta-globin translation initiation cod ... | 1981 | 6286822 |
| biological activities and spectroscopic properties of chromophoric and fluorescent analogs of adenine nucleoside and nucleotides, 2',3'-o-(2,4,6-trinitrocyclohexadienylidene) adenosine derivatives. | the ribose-modified chromophoric and fluorescent analog of atp 2',3'-o-(2,4,6-trinitrocyclohexadienylidene) adenosine 5'-triphosphate (tnp-atp) has been synthesized previously (hiratsuka, t., and uchida, k. (1973) biochim. biophys. acta 320, 635-647 and hiratsuka, t. (1976) biochim. biophys. acta 453, 293-297). in the present study, four tnp-derivatives of atp, adp, amp and adenosine were synthesized and compared for several chemical, spectral and enzymatic properties. their visible absorption a ... | 1982 | 6295507 |
| automatic and manual latex agglutination tests for measurement of cholera toxin and heat-labile enterotoxin of escherichia coli. | automated and manual latex agglutination methods were employed to measure cholera toxin (ct), heat-labile enterotoxin (lt) of escherichia coli, and their subunits a and b. dow polystyrene latex particles (diameter, 0.22 microns) and polystyrene-chlorostyrene latex particles (diameter, 1 micron) were sensitized by rabbit-specific immunoglobulin for each antigen and used as the reagents of the automated and manual agglutination tests, respectively. automated agglutination was performed by a nephel ... | 1983 | 6298275 |
| restriction analysis of dna from treponema pallidum, the causative agent of syphilis. | when purified dna from pathogenic treponema pallidum is digested with restriction endonucleases it results in the formation of discrete dna fragments which range between 2.5 to 10 kilobase pairs. no such precise fragmentation occurs with dna isolated from nonpathogenic t. pallidum. the appearance of the discrete restriction fragments from the pathogenic t. pallidum dna does not represent a contamination of satellite dna from rabbit, the host in which the organism was propagated, but rather repre ... | 1983 | 6310347 |
| interactions between entamoeba histolytica, bacteria and intestinal cells. | axenically grown pathogenic and non-pathogenic isolates of entamoeba histolytica have been shown to adhere to mammalian epithelial cells and bacteria by virtue of carbohydrate-binding proteins present on their cell surfaces. the interaction of amoeba isolates of low pathogenicity with a variety of gram-negative bacteria, mainly escherichia coli strains which are readily ingested by the amoebae after relatively short periods, significantly increased the ability of the trophozoites to: (a) destroy ... | 1983 | 6315320 |
| ratio of active to inactive forms of acyl carrier protein in escherichia coli. | acyl carrier protein (acp) functions as a cofactor in fatty acid biosynthesis due to the covalent linkage of an acyl moiety to its 4'-phosphopantetheine prosthetic group. this prosthetic group undergoes turnover in vivo and since the apoprotein is functionally inactive, the interconversion between acp and apo-acp has been considered as a possible regulatory point in lipid biosynthesis. to investigate this possibility, the ratio of acp to apo-acp was measured in escherichia coli. an apo-acp stand ... | 1983 | 6317688 |
| mechanisms of adrenocortical depression during escherichia coli shock. | the response of the adrenal cortex to corticotropin during sepsis is variable. we have previously demonstrated a significant decrease of corticosterone production by rat adrenocortical cells in response to corticotropin stimulation after incubation with septic shock plasma (sp) as compared with control plasma (cp). we have studied the mechanisms of this depression. the following defects were demonstrated. (1) cells bound less radioiodinated corticotropin analog after sp treatment (2.9 +/- 0.4 fe ... | 1984 | 6320764 |
| a bacillus subtilis secretion vector system derived from the b. subtilis alpha-amylase promoter and signal sequence region, and secretion of escherichia coli beta-lactamase by the vector system. | a secretion vector system in bacillus subtilis was constructed from the alpha-amylase promoter and signal sequence coding region of an alpha-amylase hyperproducing strain, b. subtilis na64, and the major part of the plasmid ptub4 which was derived from pub110. when an escherichia coli beta-lactamase gene, lacking its own promoter and signal sequence coding region, was introduced into the secretion vector system, beta-lactamase was expressed in b. subtilis. in addition, more than 95% of the enzym ... | 1984 | 6323395 |
| distantly related sequences in the alpha- and beta-subunits of atp synthase, myosin, kinases and other atp-requiring enzymes and a common nucleotide binding fold. | the alpha- and beta-subunits of membrane-bound atp synthase complex bind atp and adp: beta contributes to catalytic sites, and alpha may be involved in regulation of atp synthase activity. the sequences of beta-subunits are highly conserved in escherichia coli and bovine mitochondria. also alpha and beta are weakly homologous to each other throughout most of their amino acid sequences, suggesting that they have common functions in catalysis. related sequences in both alpha and beta and in other ... | 1982 | 6329717 |